Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220240 | KEH39063.1 | 56.164 | 73 | 12 | 1 | 1 | 53 | 1 | 73 | 2.23e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220240 | A0A072VC84 | 56.164 | 73 | 12 | 1 | 1 | 53 | 1 | 73 | 1.07e-15 | 74.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0204960 | Msa0220240 | 0.816905 | 4.345463e-52 | -8.615850e-47 |
| Msa0217670 | Msa0220240 | 0.816950 | 4.244490e-52 | -8.615850e-47 |
| Msa0220240 | Msa0220250 | 0.834637 | 2.671864e-56 | -8.615850e-47 |
| Msa0220240 | Msa0257490 | 0.827851 | 1.245149e-54 | -8.615850e-47 |
| Msa0220240 | Msa0261540 | 0.801091 | 1.060437e-48 | -8.615850e-47 |
| Msa0220240 | Msa0261550 | 0.825701 | 4.060839e-54 | -8.615850e-47 |
| Msa0220240 | Msa0281530 | 0.812312 | 4.517545e-51 | -8.615850e-47 |
| Msa0220240 | Msa0296790 | 0.831840 | 1.328427e-55 | -8.615850e-47 |
| Msa0220240 | Msa0304510 | 0.801738 | 7.816629e-49 | -8.615850e-47 |
| Msa0220240 | Msa0343850 | 0.829768 | 4.279527e-55 | -8.615850e-47 |
| Msa0220240 | Msa0401560 | 0.807818 | 4.198200e-50 | -8.615850e-47 |
| Msa0220240 | Msa0453680 | 0.816345 | 5.802101e-52 | -8.615850e-47 |
| Msa0220240 | Msa0542290 | 0.819836 | 9.423404e-53 | -8.615850e-47 |
| Msa0220240 | Msa0542560 | 0.801254 | 9.820768e-49 | -8.615850e-47 |
| Msa0220240 | Msa0547160 | 0.833428 | 5.363049e-56 | -8.615850e-47 |
| Msa0220240 | Msa0600080 | 0.803524 | 3.346054e-49 | -8.615850e-47 |
| Msa0220240 | Msa0604290 | 0.825010 | 5.917255e-54 | -8.615850e-47 |
| Msa0220240 | Msa0642970 | 0.807523 | 4.848370e-50 | -8.615850e-47 |
| Msa0220240 | Msa0645530 | 0.809008 | 2.339262e-50 | -8.615850e-47 |
| Msa0220240 | Msa0682680 | 0.831805 | 1.355059e-55 | -8.615850e-47 |
| Msa0220240 | Msa0685090 | 0.812761 | 3.602715e-51 | -8.615850e-47 |
| Msa0220240 | Msa0721280 | 0.819204 | 1.313103e-52 | -8.615850e-47 |
| Msa0220240 | Msa0773850 | 0.809083 | 2.254186e-50 | -8.615850e-47 |
| Msa0220240 | Msa0933380 | 0.807509 | 4.882238e-50 | -8.615850e-47 |
| Msa0220240 | Msa0971160 | 0.811153 | 8.072454e-51 | -8.615850e-47 |
| Msa0220240 | Msa1212950 | 0.800391 | 1.473289e-48 | -8.615850e-47 |
| Msa0220240 | Msa1230080 | 0.805635 | 1.213825e-49 | -8.615850e-47 |
| Msa0220240 | Msa1272620 | 0.809612 | 1.736315e-50 | -8.615850e-47 |
| Msa0220240 | Msa1310400 | 0.802725 | 4.895137e-49 | -8.615850e-47 |
| Msa0220240 | Msa1337170 | 0.822692 | 2.067964e-53 | -8.615850e-47 |
| Msa0220240 | Msa1357890 | 0.818425 | 1.973346e-52 | -8.615850e-47 |
| Msa0220240 | Msa1398090 | 0.822762 | 1.991797e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220240 | MtrunA17_Chr2g0323751 | 56.164 | 73 | 12 | 1 | 1 | 53 | 1 | 73 | 2.05e-19 | 74.3 |
| Msa0220240 | MtrunA17_Chr2g0323741 | 58.904 | 73 | 9 | 2 | 1 | 53 | 1 | 72 | 1.10e-18 | 72.4 |
| Msa0220240 | MtrunA17_Chr2g0323761 | 55.556 | 72 | 10 | 2 | 1 | 53 | 1 | 69 | 3.54e-16 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTTGCACTTATGAAT+TGG | 0.356583 | 2_1:-68448140 | None:intergenic |
| TGATTTACTTGAACCGTGTT+TGG | 0.371273 | 2_1:+68448078 | Msa0220240:CDS |
| GTAATTAGTGACCGTTTGAT+AGG | 0.420509 | 2_1:+68448708 | Msa0220240:intron |
| TGCAAATTCCTCCTATCAAA+CGG | 0.506323 | 2_1:-68448719 | None:intergenic |
| GTTGTCCAGCGTCGTTGTAT+CGG | 0.511337 | 2_1:+68448172 | Msa0220240:CDS |
| GATGGCAGCAACTGTGGCTG+TGG | 0.512055 | 2_1:+68448100 | Msa0220240:CDS |
| ATTAGTGACCGTTTGATAGG+AGG | 0.540354 | 2_1:+68448711 | Msa0220240:intron |
| GCAACTGTGGCTGTGGCACA+AGG | 0.545391 | 2_1:+68448107 | Msa0220240:CDS |
| GCAACCGATGTGATGAGACT+TGG | 0.584158 | 2_1:+68448739 | Msa0220240:CDS |
| TGCTTCCGATACAACGACGC+TGG | 0.592056 | 2_1:-68448177 | None:intergenic |
| TTACTTGAACCGTGTTTGGA+TGG | 0.612225 | 2_1:+68448082 | Msa0220240:CDS |
| GATACAACGACGCTGGACAA+CGG | 0.631291 | 2_1:-68448170 | None:intergenic |
| TTTCGCCTCAGAACTCATGA+CGG | 0.633585 | 2_1:-68448201 | None:intergenic |
| TGTTTGGATGGCAGCAACTG+TGG | 0.640468 | 2_1:+68448094 | Msa0220240:CDS |
| CAGTTGCTGCCATCCAAACA+CGG | 0.657329 | 2_1:-68448091 | None:intergenic |
| AGCAACCGTCATGAGTTCTG+AGG | 0.671729 | 2_1:+68448196 | Msa0220240:CDS |
| GTATCCAAGTCTCATCACAT+CGG | 0.706409 | 2_1:-68448743 | None:intergenic |
| ACTTATGAATTGGATCAGTG+TGG | 0.757374 | 2_1:-68448130 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAAGAAAACAACATATA+GGG | - | chr2_1:68448350-68448369 | None:intergenic | 20.0% |
| !!! | TATATGTTGTTTTCTTGAAT+GGG | + | chr2_1:68448350-68448369 | Msa0220240:intron | 20.0% |
| !! | TGCTAAAAGTTAGTTAATTT+AGG | + | chr2_1:68448663-68448682 | Msa0220240:intron | 20.0% |
| ! | AATTCATGTACATAACTTTC+TGG | - | chr2_1:68448256-68448275 | None:intergenic | 25.0% |
| ! | AAGTTATGTACATGAATTGT+TGG | + | chr2_1:68448258-68448277 | Msa0220240:intron | 25.0% |
| ! | AGTTATGTACATGAATTGTT+GGG | + | chr2_1:68448259-68448278 | Msa0220240:intron | 25.0% |
| ! | CATTCAAGAAAACAACATAT+AGG | - | chr2_1:68448351-68448370 | None:intergenic | 25.0% |
| !!! | CTATATGTTGTTTTCTTGAA+TGG | + | chr2_1:68448349-68448368 | Msa0220240:intron | 25.0% |
| !! | TTTTCTTGAATGGGAAATAT+TGG | + | chr2_1:68448359-68448378 | Msa0220240:intron | 25.0% |
| ! | ATATTGGAAAAAGGAAACAA+TGG | + | chr2_1:68448375-68448394 | Msa0220240:intron | 25.0% |
| ! | ATCTTCTTCATTCAAATTGT+GGG | + | chr2_1:68448436-68448455 | Msa0220240:intron | 25.0% |
| ! | TGCAAAATTTCCTTGAATAT+TGG | + | chr2_1:68448481-68448500 | Msa0220240:intron | 25.0% |
| ! | TATACAAGTGAATGAAATCA+AGG | + | chr2_1:68448514-68448533 | Msa0220240:intron | 25.0% |
| ! | TCTAAGGAATATTTCATGAT+AGG | - | chr2_1:68448571-68448590 | None:intergenic | 25.0% |
| ! | GTAGACTTATTTATAGTCTA+AGG | - | chr2_1:68448587-68448606 | None:intergenic | 25.0% |
| !!! | TTGATGGGTATTTTTTCATT+TGG | + | chr2_1:68448637-68448656 | Msa0220240:intron | 25.0% |
| GTTATGTACATGAATTGTTG+GGG | + | chr2_1:68448260-68448279 | Msa0220240:intron | 30.0% | |
| GAATGGGAAATATTGGAAAA+AGG | + | chr2_1:68448366-68448385 | Msa0220240:intron | 30.0% | |
| GATCTTCTTCATTCAAATTG+TGG | + | chr2_1:68448435-68448454 | Msa0220240:intron | 30.0% | |
| TTTGACTCAACCAATATTCA+AGG | - | chr2_1:68448494-68448513 | None:intergenic | 30.0% | |
| AAATCAAAGAGAAGCAAAAG+AGG | + | chr2_1:68448537-68448556 | Msa0220240:intron | 30.0% | |
| AATCAAAGAGAAGCAAAAGA+GGG | + | chr2_1:68448538-68448557 | Msa0220240:intron | 30.0% | |
| TGATTTACTTGAACCGTGTT+TGG | + | chr2_1:68448078-68448097 | Msa0220240:CDS | 35.0% | |
| ACTTATGAATTGGATCAGTG+TGG | - | chr2_1:68448133-68448152 | None:intergenic | 35.0% | |
| !! | GCTGATTTGCACTTATGAAT+TGG | - | chr2_1:68448143-68448162 | None:intergenic | 35.0% |
| ! | AACTTTCTGGAGAGAATCAT+CGG | - | chr2_1:68448243-68448262 | None:intergenic | 35.0% |
| AGACGTATCAAATTGTCCAA+GGG | - | chr2_1:68448405-68448424 | None:intergenic | 35.0% | |
| TAGACGTATCAAATTGTCCA+AGG | - | chr2_1:68448406-68448425 | None:intergenic | 35.0% | |
| GCAACTTGAACAAATATTCC+TGG | - | chr2_1:68448464-68448483 | None:intergenic | 35.0% | |
| TTGATCAGCTCACTAATCAT+TGG | - | chr2_1:68448615-68448634 | None:intergenic | 35.0% | |
| AGTGAGCTGATCAAAATTGA+TGG | + | chr2_1:68448621-68448640 | Msa0220240:intron | 35.0% | |
| GTGAGCTGATCAAAATTGAT+GGG | + | chr2_1:68448622-68448641 | Msa0220240:intron | 35.0% | |
| GTAATTAGTGACCGTTTGAT+AGG | + | chr2_1:68448708-68448727 | Msa0220240:intron | 35.0% | |
| TGCAAATTCCTCCTATCAAA+CGG | - | chr2_1:68448722-68448741 | None:intergenic | 35.0% | |
| TTACTTGAACCGTGTTTGGA+TGG | + | chr2_1:68448082-68448101 | Msa0220240:CDS | 40.0% | |
| TACATGAATTGTTGGGGTCA+AGG | + | chr2_1:68448266-68448285 | Msa0220240:intron | 40.0% | |
| ! | AGAAGCCAGAACTGATTCTA+AGG | + | chr2_1:68448312-68448331 | Msa0220240:intron | 40.0% |
| ! | GAAGCCAGAACTGATTCTAA+GGG | + | chr2_1:68448313-68448332 | Msa0220240:intron | 40.0% |
| AGGAAACAATGGAATTCCCT+TGG | + | chr2_1:68448386-68448405 | Msa0220240:intron | 40.0% | |
| TCATTCAAATTGTGGGATCC+AGG | + | chr2_1:68448443-68448462 | Msa0220240:intron | 40.0% | |
| ATTAGTGACCGTTTGATAGG+AGG | + | chr2_1:68448711-68448730 | Msa0220240:intron | 40.0% | |
| GTATCCAAGTCTCATCACAT+CGG | - | chr2_1:68448746-68448765 | None:intergenic | 40.0% | |
| TTTCGCCTCAGAACTCATGA+CGG | - | chr2_1:68448204-68448223 | None:intergenic | 45.0% | |
| ATTGTTGGGGTCAAGGCTAA+TGG | + | chr2_1:68448273-68448292 | Msa0220240:intron | 45.0% | |
| CAAGGCTAATGGTGACAACA+AGG | + | chr2_1:68448284-68448303 | Msa0220240:intron | 45.0% | |
| TGCACCCTTAGAATCAGTTC+TGG | - | chr2_1:68448320-68448339 | None:intergenic | 45.0% | |
| CAGTTGCTGCCATCCAAACA+CGG | - | chr2_1:68448094-68448113 | None:intergenic | 50.0% | |
| TGTTTGGATGGCAGCAACTG+TGG | + | chr2_1:68448094-68448113 | Msa0220240:CDS | 50.0% | |
| GATACAACGACGCTGGACAA+CGG | - | chr2_1:68448173-68448192 | None:intergenic | 50.0% | |
| GTTGTCCAGCGTCGTTGTAT+CGG | + | chr2_1:68448172-68448191 | Msa0220240:CDS | 50.0% | |
| AGCAACCGTCATGAGTTCTG+AGG | + | chr2_1:68448196-68448215 | Msa0220240:CDS | 50.0% | |
| GCAACCGATGTGATGAGACT+TGG | + | chr2_1:68448739-68448758 | Msa0220240:CDS | 50.0% | |
| TGCTTCCGATACAACGACGC+TGG | - | chr2_1:68448180-68448199 | None:intergenic | 55.0% | |
| GATGGCAGCAACTGTGGCTG+TGG | + | chr2_1:68448100-68448119 | Msa0220240:CDS | 60.0% | |
| GCAACTGTGGCTGTGGCACA+AGG | + | chr2_1:68448107-68448126 | Msa0220240:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 68448077 | 68448773 | 68448077 | ID=Msa0220240;Name=Msa0220240 |
| chr2_1 | mRNA | 68448077 | 68448773 | 68448077 | ID=Msa0220240-mRNA-1;Parent=Msa0220240;Name=Msa0220240-mRNA-1;_AED=0.53;_eAED=0.53;_QI=0|0|0|0.5|0|0|2|0|69 |
| chr2_1 | exon | 68448077 | 68448228 | 68448077 | ID=Msa0220240-mRNA-1:exon:19379;Parent=Msa0220240-mRNA-1 |
| chr2_1 | exon | 68448716 | 68448773 | 68448716 | ID=Msa0220240-mRNA-1:exon:19380;Parent=Msa0220240-mRNA-1 |
| chr2_1 | CDS | 68448077 | 68448228 | 68448077 | ID=Msa0220240-mRNA-1:cds;Parent=Msa0220240-mRNA-1 |
| chr2_1 | CDS | 68448716 | 68448773 | 68448716 | ID=Msa0220240-mRNA-1:cds;Parent=Msa0220240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
