Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220250 | XP_013465029.1 | 97.468 | 79 | 2 | 0 | 1 | 79 | 9 | 87 | 3.29e-50 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220250 | A0A072VAJ9 | 97.468 | 79 | 2 | 0 | 1 | 79 | 9 | 87 | 1.57e-50 | 162 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001450 | Msa0220250 | 0.841676 | 4.112503e-58 | -8.615850e-47 |
| Msa0003700 | Msa0220250 | 0.802884 | 4.539634e-49 | -8.615850e-47 |
| Msa0011660 | Msa0220250 | -0.800360 | 1.494695e-48 | -8.615850e-47 |
| Msa0015390 | Msa0220250 | 0.810505 | 1.115058e-50 | -8.615850e-47 |
| Msa0020390 | Msa0220250 | 0.815723 | 7.989600e-52 | -8.615850e-47 |
| Msa0027260 | Msa0220250 | 0.815186 | 1.051653e-51 | -8.615850e-47 |
| Msa0065950 | Msa0220250 | 0.808814 | 2.573644e-50 | -8.615850e-47 |
| Msa0090570 | Msa0220250 | 0.810127 | 1.344967e-50 | -8.615850e-47 |
| Msa0096980 | Msa0220250 | 0.800127 | 1.667323e-48 | -8.615850e-47 |
| Msa0104550 | Msa0220250 | 0.806723 | 7.162474e-50 | -8.615850e-47 |
| Msa0127800 | Msa0220250 | 0.817068 | 3.993791e-52 | -8.615850e-47 |
| Msa0140110 | Msa0220250 | 0.814062 | 1.865059e-51 | -8.615850e-47 |
| Msa0171410 | Msa0220250 | 0.803663 | 3.131395e-49 | -8.615850e-47 |
| Msa0194480 | Msa0220250 | 0.805064 | 1.598641e-49 | -8.615850e-47 |
| Msa0204960 | Msa0220250 | 0.804731 | 1.876730e-49 | -8.615850e-47 |
| Msa0220240 | Msa0220250 | 0.834637 | 2.671864e-56 | -8.615850e-47 |
| Msa0220250 | Msa0231980 | 0.810147 | 1.331766e-50 | -8.615850e-47 |
| Msa0220250 | Msa0232050 | 0.800921 | 1.148919e-48 | -8.615850e-47 |
| Msa0220250 | Msa0257490 | 0.816635 | 4.997002e-52 | -8.615850e-47 |
| Msa0220250 | Msa0261550 | 0.883157 | 5.690193e-71 | -8.615850e-47 |
| Msa0220250 | Msa0281530 | 0.801639 | 8.187283e-49 | -8.615850e-47 |
| Msa0220250 | Msa0296790 | 0.801904 | 7.224814e-49 | -8.615850e-47 |
| Msa0220250 | Msa0304510 | 0.838807 | 2.309205e-57 | -8.615850e-47 |
| Msa0220250 | Msa0305030 | 0.946940 | 1.763643e-105 | -8.615850e-47 |
| Msa0220250 | Msa0343850 | 0.816552 | 5.215445e-52 | -8.615850e-47 |
| Msa0220250 | Msa0380300 | 0.811773 | 5.920435e-51 | -8.615850e-47 |
| Msa0220250 | Msa0415430 | 0.809583 | 1.761259e-50 | -8.615850e-47 |
| Msa0220250 | Msa0453350 | 0.802682 | 4.996234e-49 | -8.615850e-47 |
| Msa0220250 | Msa0485850 | 0.804316 | 2.290076e-49 | -8.615850e-47 |
| Msa0220250 | Msa0524500 | 0.834114 | 3.614683e-56 | -8.615850e-47 |
| Msa0220250 | Msa0542290 | 0.815838 | 7.531495e-52 | -8.615850e-47 |
| Msa0220250 | Msa0547160 | 0.806713 | 7.197673e-50 | -8.615850e-47 |
| Msa0220250 | Msa0556060 | 0.821315 | 4.309434e-53 | -8.615850e-47 |
| Msa0220250 | Msa0642970 | 0.826840 | 2.175483e-54 | -8.615850e-47 |
| Msa0220250 | Msa0683730 | 0.800343 | 1.506925e-48 | -8.615850e-47 |
| Msa0220250 | Msa0698540 | 0.814747 | 1.316094e-51 | -8.615850e-47 |
| Msa0220250 | Msa0702470 | 0.803987 | 2.681862e-49 | -8.615850e-47 |
| Msa0220250 | Msa0722020 | 0.807590 | 4.692867e-50 | -8.615850e-47 |
| Msa0220250 | Msa0727150 | 0.809988 | 1.441290e-50 | -8.615850e-47 |
| Msa0220250 | Msa0729720 | 0.810861 | 9.338194e-51 | -8.615850e-47 |
| Msa0220250 | Msa0737600 | 0.803790 | 2.947037e-49 | -8.615850e-47 |
| Msa0220250 | Msa0763020 | 0.811885 | 5.597872e-51 | -8.615850e-47 |
| Msa0220250 | Msa0763070 | 0.818553 | 1.845845e-52 | -8.615850e-47 |
| Msa0220250 | Msa0801110 | 0.808245 | 3.404326e-50 | -8.615850e-47 |
| Msa0220250 | Msa0808080 | 0.800321 | 1.522185e-48 | -8.615850e-47 |
| Msa0220250 | Msa0835280 | 0.801986 | 6.950225e-49 | -8.615850e-47 |
| Msa0220250 | Msa0847080 | 0.803722 | 3.044284e-49 | -8.615850e-47 |
| Msa0220250 | Msa0933380 | 0.808201 | 3.479121e-50 | -8.615850e-47 |
| Msa0220250 | Msa0971160 | 0.806295 | 8.816690e-50 | -8.615850e-47 |
| Msa0220250 | Msa0999050 | -0.811529 | 6.688329e-51 | -8.615850e-47 |
| Msa0220250 | Msa1023180 | 0.810350 | 1.203979e-50 | -8.615850e-47 |
| Msa0220250 | Msa1043050 | 0.816181 | 6.312836e-52 | -8.615850e-47 |
| Msa0220250 | Msa1150710 | 0.825141 | 5.509628e-54 | -8.615850e-47 |
| Msa0220250 | Msa1204250 | 0.800569 | 1.355473e-48 | -8.615850e-47 |
| Msa0220250 | Msa1239410 | 0.803017 | 4.260100e-49 | -8.615850e-47 |
| Msa0220250 | Msa1254730 | 0.806923 | 6.497325e-50 | -8.615850e-47 |
| Msa0220250 | Msa1256470 | 0.826519 | 2.594685e-54 | -8.615850e-47 |
| Msa0220250 | Msa1275360 | 0.813365 | 2.656452e-51 | -8.615850e-47 |
| Msa0220250 | Msa1286800 | 0.802842 | 4.630337e-49 | -8.615850e-47 |
| Msa0220250 | Msa1294820 | 0.812680 | 3.752364e-51 | -8.615850e-47 |
| Msa0220250 | Msa1296830 | 0.817239 | 3.655255e-52 | -8.615850e-47 |
| Msa0220250 | Msa1333110 | 0.803732 | 3.028925e-49 | -8.615850e-47 |
| Msa0220250 | Msa1365800 | 0.803980 | 2.690954e-49 | -8.615850e-47 |
| Msa0220250 | Msa1367590 | 0.804710 | 1.895581e-49 | -8.615850e-47 |
| Msa0220250 | Msa1368530 | 0.802098 | 6.591681e-49 | -8.615850e-47 |
| Msa0220250 | Msa1376650 | 0.822340 | 2.495779e-53 | -8.615850e-47 |
| Msa0220250 | Msa1385770 | 0.805017 | 1.634942e-49 | -8.615850e-47 |
| Msa0220250 | Msa1409240 | 0.806536 | 7.842069e-50 | -8.615850e-47 |
| Msa0220250 | Msa1434140 | 0.803031 | 4.232084e-49 | -8.615850e-47 |
| Msa0220250 | Msa1448630 | 0.801097 | 1.057366e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220250 | MtrunA17_Chr2g0323761 | 97.468 | 79 | 2 | 0 | 1 | 79 | 9 | 87 | 3.03e-54 | 162 |
| Msa0220250 | MtrunA17_Chr2g0323741 | 79.268 | 82 | 14 | 2 | 1 | 79 | 9 | 90 | 1.28e-41 | 130 |
| Msa0220250 | MtrunA17_Chr2g0323751 | 74.699 | 83 | 17 | 2 | 1 | 79 | 9 | 91 | 3.21e-39 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220250 | AT4G28240.1 | 42.857 | 84 | 37 | 3 | 1 | 79 | 9 | 86 | 1.11e-13 | 60.5 |
Find 29 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGAGACGAGTTCGGTTT+TGG | 0.261084 | 2_1:-68454796 | None:intergenic |
| TCCGATCTCCGGCCGTTGTC+CGG | 0.378956 | 2_1:+68454833 | Msa0220250:CDS |
| AAGTTATGTACATGAATTGC+TGG | 0.397828 | 2_1:+68454924 | Msa0220250:CDS |
| GCGCAAGGCCACACCGATCC+AGG | 0.445476 | 2_1:+68454740 | Msa0220250:CDS |
| CCGGAGATCGGATAAACTTC+CGG | 0.452248 | 2_1:-68454822 | None:intergenic |
| GTTGTCCGGCGTTGTTGTAT+CGG | 0.459575 | 2_1:+68454847 | Msa0220250:CDS |
| CAACAACGCCGGACAACGGC+CGG | 0.460383 | 2_1:-68454841 | None:intergenic |
| CGGCGGAGAAGAGACGAGTT+CGG | 0.469225 | 2_1:-68454802 | None:intergenic |
| GTGGCCTTGCGCCACCGCTA+CGG | 0.494254 | 2_1:-68454729 | None:intergenic |
| TTTGCACTTGTGGCCTGGAT+CGG | 0.503366 | 2_1:-68454753 | None:intergenic |
| GAAGAACCGATGATTCTCTT+CGG | 0.508064 | 2_1:+68454900 | Msa0220250:CDS |
| GCCGGACAACGGCCGGAGAT+CGG | 0.526728 | 2_1:-68454834 | None:intergenic |
| TACATGAATTGCTGGGGTCA+AGG | 0.532348 | 2_1:+68454932 | Msa0220250:CDS |
| AGTTATGTACATGAATTGCT+GGG | 0.535583 | 2_1:+68454925 | Msa0220250:CDS |
| CCGGAAGTTTATCCGATCTC+CGG | 0.544467 | 2_1:+68454822 | Msa0220250:CDS |
| GCAACCGTAGCGGTGGCGCA+AGG | 0.545121 | 2_1:+68454725 | Msa0220250:CDS |
| AACTTTCCGAAGAGAATCAT+CGG | 0.563956 | 2_1:-68454906 | None:intergenic |
| GATACAACAACGCCGGACAA+CGG | 0.584437 | 2_1:-68454845 | None:intergenic |
| GATGGCGGCAACCGTAGCGG+TGG | 0.585060 | 2_1:+68454718 | None:intergenic |
| CGAACTCGTCTCTTCTCCGC+CGG | 0.599264 | 2_1:+68454803 | Msa0220250:CDS |
| CACAAGTGCAAAACAGCCGT+TGG | 0.609626 | 2_1:+68454764 | Msa0220250:CDS |
| GTCCGGCGTTGTTGTATCGG+AGG | 0.610205 | 2_1:+68454850 | Msa0220250:CDS |
| TGTATCGGAGGTGAGTTCTG+AGG | 0.621033 | 2_1:+68454862 | Msa0220250:CDS |
| ATCGGAGGTGAGTTCTGAGG+CGG | 0.654083 | 2_1:+68454865 | Msa0220250:CDS |
| TTGGATGGCGGCAACCGTAG+CGG | 0.657920 | 2_1:+68454715 | None:intergenic |
| CACCTCCGATACAACAACGC+CGG | 0.661000 | 2_1:-68454852 | None:intergenic |
| ACTTGTGGCCTGGATCGGTG+TGG | 0.680459 | 2_1:-68454748 | None:intergenic |
| GTTATGTACATGAATTGCTG+GGG | 0.680638 | 2_1:+68454926 | Msa0220250:CDS |
| GAGATCGGATAAACTTCCGG+CGG | 0.743399 | 2_1:-68454819 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTTATGTACATGAATTGC+TGG | + | chr2_1:68454924-68454943 | Msa0220250:CDS | 30.0% | |
| AGTTATGTACATGAATTGCT+GGG | + | chr2_1:68454925-68454944 | Msa0220250:CDS | 30.0% | |
| !! | GTTTTGGTGAATAGATCCAA+CGG | - | chr2_1:68454783-68454802 | None:intergenic | 35.0% |
| AACTTTCCGAAGAGAATCAT+CGG | - | chr2_1:68454909-68454928 | None:intergenic | 35.0% | |
| GTTATGTACATGAATTGCTG+GGG | + | chr2_1:68454926-68454945 | Msa0220250:CDS | 35.0% | |
| GAAGAACCGATGATTCTCTT+CGG | + | chr2_1:68454900-68454919 | Msa0220250:CDS | 40.0% | |
| ! | AGAAGAGACGAGTTCGGTTT+TGG | - | chr2_1:68454799-68454818 | None:intergenic | 45.0% |
| TACATGAATTGCTGGGGTCA+AGG | + | chr2_1:68454932-68454951 | Msa0220250:CDS | 45.0% | |
| TTTGCACTTGTGGCCTGGAT+CGG | - | chr2_1:68454756-68454775 | None:intergenic | 50.0% | |
| !! | CAACGGCTGTTTTGCACTTG+TGG | - | chr2_1:68454766-68454785 | None:intergenic | 50.0% |
| CACAAGTGCAAAACAGCCGT+TGG | + | chr2_1:68454764-68454783 | Msa0220250:CDS | 50.0% | |
| GAGATCGGATAAACTTCCGG+CGG | - | chr2_1:68454822-68454841 | None:intergenic | 50.0% | |
| CCGGAGATCGGATAAACTTC+CGG | - | chr2_1:68454825-68454844 | None:intergenic | 50.0% | |
| CCGGAAGTTTATCCGATCTC+CGG | + | chr2_1:68454822-68454841 | Msa0220250:CDS | 50.0% | |
| GATACAACAACGCCGGACAA+CGG | - | chr2_1:68454848-68454867 | None:intergenic | 50.0% | |
| GTTGTCCGGCGTTGTTGTAT+CGG | + | chr2_1:68454847-68454866 | Msa0220250:CDS | 50.0% | |
| TGTATCGGAGGTGAGTTCTG+AGG | + | chr2_1:68454862-68454881 | Msa0220250:CDS | 50.0% | |
| !! | GCTGTTTTGCACTTGTGGCC+TGG | - | chr2_1:68454761-68454780 | None:intergenic | 55.0% |
| CACCTCCGATACAACAACGC+CGG | - | chr2_1:68454855-68454874 | None:intergenic | 55.0% | |
| !! | ATCGGAGGTGAGTTCTGAGG+CGG | + | chr2_1:68454865-68454884 | Msa0220250:CDS | 55.0% |
| ACTTGTGGCCTGGATCGGTG+TGG | - | chr2_1:68454751-68454770 | None:intergenic | 60.0% | |
| CGGCGGAGAAGAGACGAGTT+CGG | - | chr2_1:68454805-68454824 | None:intergenic | 60.0% | |
| CGAACTCGTCTCTTCTCCGC+CGG | + | chr2_1:68454803-68454822 | Msa0220250:CDS | 60.0% | |
| GTCCGGCGTTGTTGTATCGG+AGG | + | chr2_1:68454850-68454869 | Msa0220250:CDS | 60.0% | |
| TCCGATCTCCGGCCGTTGTC+CGG | + | chr2_1:68454833-68454852 | Msa0220250:CDS | 65.0% | |
| CAACAACGCCGGACAACGGC+CGG | - | chr2_1:68454844-68454863 | None:intergenic | 65.0% | |
| GCAACCGTAGCGGTGGCGCA+AGG | + | chr2_1:68454725-68454744 | Msa0220250:CDS | 70.0% | |
| ! | GTGGCCTTGCGCCACCGCTA+CGG | - | chr2_1:68454732-68454751 | None:intergenic | 70.0% |
| GCGCAAGGCCACACCGATCC+AGG | + | chr2_1:68454740-68454759 | Msa0220250:CDS | 70.0% | |
| GCCGGACAACGGCCGGAGAT+CGG | - | chr2_1:68454837-68454856 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 68454719 | 68454958 | 68454719 | ID=Msa0220250;Name=Msa0220250 |
| chr2_1 | mRNA | 68454719 | 68454958 | 68454719 | ID=Msa0220250-mRNA-1;Parent=Msa0220250;Name=Msa0220250-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|79 |
| chr2_1 | exon | 68454719 | 68454958 | 68454719 | ID=Msa0220250-mRNA-1:exon:19359;Parent=Msa0220250-mRNA-1 |
| chr2_1 | CDS | 68454719 | 68454958 | 68454719 | ID=Msa0220250-mRNA-1:cds;Parent=Msa0220250-mRNA-1 |
| Gene Sequence |
| Protein sequence |
