AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0453680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0453680 MtrunA17_Chr3g0099751 95.146 103 4 1 1 102 1 103 2.01e-67 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGAATTTGTGAGTTTAA+TGG 0.250143 3_3:+44365227 Msa0453680:three_prime_UTR
AACATGCTTTGATAGAATTA+TGG 0.258618 3_3:+44364846 Msa0453680:intron
GTGAAATAATAAGAATTTCT+TGG 0.259819 3_3:+44365259 Msa0453680:three_prime_UTR
AGTGAAGAAGAGTGAGGAAT+TGG 0.296391 3_3:-44364122 None:intergenic
AATATTGAAGTTGGGAGAAT+GGG 0.360100 3_3:-44364036 None:intergenic
GTACAAGGAGCATTGATCTT+AGG 0.360884 3_3:+44365061 Msa0453680:exon
AAAGGAAAGTCATTTGATAA+AGG 0.376794 3_3:+44364884 Msa0453680:CDS
AAAATTAAATGGTACCTTGA+TGG 0.391314 3_3:-44364196 None:intergenic
GTCTACACATATATATCTAT+TGG 0.392894 3_3:-44363903 None:intergenic
GTGTGCAAAGTTGTCCGTTT+AGG 0.396085 3_3:+44363947 Msa0453680:five_prime_UTR
TGGAGACTTCATGAATCATT+AGG 0.408038 3_3:+44365322 Msa0453680:three_prime_UTR
TGAAATTCTTCAGCTGTAGA+TGG 0.429413 3_3:-44364915 None:intergenic
AACACTTCATGCAACCTAAA+CGG 0.458091 3_3:-44363961 None:intergenic
GAGAATGGGGATGTGTCTAT+AGG 0.462776 3_3:-44364022 None:intergenic
AAATATTGAAGTTGGGAGAA+TGG 0.473143 3_3:-44364037 None:intergenic
GTGAAGAAGAGTGAGGAATT+GGG 0.473632 3_3:-44364121 None:intergenic
TTGTAGAAGAATGGTCTAGA+GGG 0.502419 3_3:-44364171 None:intergenic
TGCTGCTGAAGAAGCTGCTA+AGG 0.518120 3_3:+44365007 Msa0453680:CDS
AAGCTGCTAAGGGTAATTCA+AGG 0.521770 3_3:+44365018 Msa0453680:CDS
TGGACAAGAAGTGAAGGAAA+AGG 0.524067 3_3:+44364866 Msa0453680:CDS
TTGATGGAGTTGTAGAAGAA+TGG 0.525498 3_3:-44364180 None:intergenic
GTTGTAGAAGAATGGTCTAG+AGG 0.544303 3_3:-44364172 None:intergenic
ATAAACAAATGAGTTAGTAA+AGG 0.545126 3_3:-44365345 None:intergenic
GAGAGTAACCATACCATGGC+TGG 0.545967 3_3:+44364096 Msa0453680:exon
ATTGGGCACCAGCCATGGTA+TGG 0.547369 3_3:-44364104 None:intergenic
GAGGAATTGGGCACCAGCCA+TGG 0.552723 3_3:-44364109 None:intergenic
GCACACTGCACCCTCACATA+GGG 0.558975 3_3:-44363930 None:intergenic
TTACTCTCTCTCTGAGGAGG+AGG 0.560010 3_3:-44364081 None:intergenic
AATGGGGATGTGTCTATAGG+TGG 0.571409 3_3:-44364019 None:intergenic
TACACGTGGTGTTGTTGAAG+TGG 0.573045 3_3:-44363984 None:intergenic
TGCACACTGCACCCTCACAT+AGG 0.585993 3_3:-44363931 None:intergenic
GTAGCAGAGGAGAAAACCAA+TGG 0.586494 3_3:+44364942 Msa0453680:CDS
AGAATTTCAGAGAGTAGCAG+AGG 0.599232 3_3:+44364929 Msa0453680:CDS
TGGTTACTCTCTCTCTGAGG+AGG 0.604180 3_3:-44364084 None:intergenic
ATTCTTCTACAACTCCATCA+AGG 0.606836 3_3:+44364182 Msa0453680:CDS
GAATTATGGACAAGAAGTGA+AGG 0.611950 3_3:+44364860 Msa0453680:intron
ATATTGAAGTTGGGAGAATG+GGG 0.618895 3_3:-44364035 None:intergenic
AAATGTTAAGAACAAGTACA+AGG 0.627029 3_3:+44365046 Msa0453680:CDS
CACTGCACCCTCACATAGGG+TGG 0.638652 3_3:-44363927 None:intergenic
AAGATGAGTGAAGAAGAGTG+AGG 0.651606 3_3:-44364128 None:intergenic
GCTGCTGAAGAAGCTGCTAA+GGG 0.655312 3_3:+44365008 Msa0453680:CDS
GTATGGTTACTCTCTCTCTG+AGG 0.669597 3_3:-44364087 None:intergenic
GAGAGAGAGTAACCATACCA+TGG 0.690232 3_3:+44364092 Msa0453680:exon
TGTAGACACCACCCTATGTG+AGG 0.695960 3_3:+44363919 Msa0453680:five_prime_UTR
GGAAGCTAGTGATGTACACG+TGG 0.764840 3_3:-44363998 None:intergenic
GTAGACACCACCCTATGTGA+GGG 0.775960 3_3:+44363920 Msa0453680:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATTAAAAACATCAA+GGG - chr3_3:44364225-44364244 None:intergenic 10.0%
!!! TTTTTTTTCTTTCAATTAAT+TGG + chr3_3:44364253-44364272 Msa0453680:intron 10.0%
!!! TTTTAAAAAATCACATAATT+AGG - chr3_3:44364758-44364777 None:intergenic 10.0%
!! AATTTAATTAAAAACATCAA+GGG - chr3_3:44364225-44364244 None:intergenic 10.0%
!!! TTTTTTTTCTTTCAATTAAT+TGG + chr3_3:44364253-44364272 Msa0453680:intron 10.0%
!!! TTTTAAAAAATCACATAATT+AGG - chr3_3:44364758-44364777 None:intergenic 10.0%
!! CAATTTAATTAAAAACATCA+AGG - chr3_3:44364226-44364245 None:intergenic 15.0%
!!! TTAAATTTTAGTTTGTACTT+TGG + chr3_3:44364383-44364402 Msa0453680:intron 15.0%
!! CAATTTAATTAAAAACATCA+AGG - chr3_3:44364226-44364245 None:intergenic 15.0%
!!! TTAAATTTTAGTTTGTACTT+TGG + chr3_3:44364383-44364402 Msa0453680:intron 15.0%
!! AGAGTAAAAATATTGAAGTT+GGG - chr3_3:44364047-44364066 None:intergenic 20.0%
!! ATCAAGGGATAAAAATTAAA+TGG - chr3_3:44364210-44364229 None:intergenic 20.0%
!! AAAATCTTTACTTTCTTGAT+GGG - chr3_3:44364562-44364581 None:intergenic 20.0%
!! AAAAATCTTTACTTTCTTGA+TGG - chr3_3:44364563-44364582 None:intergenic 20.0%
!! CAATAATTAATAGACAACTA+CGG - chr3_3:44364818-44364837 None:intergenic 20.0%
!!! TTGTTTTGTAAGTTAGAATA+AGG + chr3_3:44365116-44365135 Msa0453680:three_prime_UTR 20.0%
!! TTATGAATTTGTGAGTTTAA+TGG + chr3_3:44365227-44365246 Msa0453680:three_prime_UTR 20.0%
!! GTGAAATAATAAGAATTTCT+TGG + chr3_3:44365259-44365278 Msa0453680:three_prime_UTR 20.0%
!!! CTCCATTTTTTTTATTTAGA+TGG + chr3_3:44365302-44365321 Msa0453680:three_prime_UTR 20.0%
!! CTCCATCTAAATAAAAAAAA+TGG - chr3_3:44365307-44365326 None:intergenic 20.0%
!! AGAGTAAAAATATTGAAGTT+GGG - chr3_3:44364047-44364066 None:intergenic 20.0%
!! ATCAAGGGATAAAAATTAAA+TGG - chr3_3:44364210-44364229 None:intergenic 20.0%
!! AAAATCTTTACTTTCTTGAT+GGG - chr3_3:44364562-44364581 None:intergenic 20.0%
!! AAAAATCTTTACTTTCTTGA+TGG - chr3_3:44364563-44364582 None:intergenic 20.0%
!! CAATAATTAATAGACAACTA+CGG - chr3_3:44364818-44364837 None:intergenic 20.0%
!!! TTGTTTTGTAAGTTAGAATA+AGG + chr3_3:44365116-44365135 Msa0453680:three_prime_UTR 20.0%
!! TTATGAATTTGTGAGTTTAA+TGG + chr3_3:44365227-44365246 Msa0453680:three_prime_UTR 20.0%
!! GTGAAATAATAAGAATTTCT+TGG + chr3_3:44365259-44365278 Msa0453680:three_prime_UTR 20.0%
!!! CTCCATTTTTTTTATTTAGA+TGG + chr3_3:44365302-44365321 Msa0453680:three_prime_UTR 20.0%
!! CTCCATCTAAATAAAAAAAA+TGG - chr3_3:44365307-44365326 None:intergenic 20.0%
! GTCTACACATATATATCTAT+TGG - chr3_3:44363906-44363925 None:intergenic 25.0%
! GAGAGTAAAAATATTGAAGT+TGG - chr3_3:44364048-44364067 None:intergenic 25.0%
! AAAATTAAATGGTACCTTGA+TGG - chr3_3:44364199-44364218 None:intergenic 25.0%
! AACTCTACATGCAATAATTA+AGG - chr3_3:44364454-44364473 None:intergenic 25.0%
! GAAAATTAAAGGTTGAAAAG+TGG + chr3_3:44364513-44364532 Msa0453680:intron 25.0%
! TTGAGTAAAGATGTCAAATT+TGG - chr3_3:44364597-44364616 None:intergenic 25.0%
! ATTTGACATCTTTACTCAAA+TGG + chr3_3:44364598-44364617 Msa0453680:intron 25.0%
! TTTGACATCTTTACTCAAAT+GGG + chr3_3:44364599-44364618 Msa0453680:intron 25.0%
! AACATGCTTTGATAGAATTA+TGG + chr3_3:44364846-44364865 Msa0453680:intron 25.0%
! AAAGGAAAGTCATTTGATAA+AGG + chr3_3:44364884-44364903 Msa0453680:CDS 25.0%
! AAATGTTAAGAACAAGTACA+AGG + chr3_3:44365046-44365065 Msa0453680:CDS 25.0%
!! TAGCAATTACACAAACTTTT+TGG - chr3_3:44365157-44365176 None:intergenic 25.0%
! GTCTACACATATATATCTAT+TGG - chr3_3:44363906-44363925 None:intergenic 25.0%
! GAGAGTAAAAATATTGAAGT+TGG - chr3_3:44364048-44364067 None:intergenic 25.0%
! AAAATTAAATGGTACCTTGA+TGG - chr3_3:44364199-44364218 None:intergenic 25.0%
! AACTCTACATGCAATAATTA+AGG - chr3_3:44364454-44364473 None:intergenic 25.0%
! GAAAATTAAAGGTTGAAAAG+TGG + chr3_3:44364513-44364532 Msa0453680:intron 25.0%
! TTGAGTAAAGATGTCAAATT+TGG - chr3_3:44364597-44364616 None:intergenic 25.0%
! ATTTGACATCTTTACTCAAA+TGG + chr3_3:44364598-44364617 Msa0453680:intron 25.0%
! TTTGACATCTTTACTCAAAT+GGG + chr3_3:44364599-44364618 Msa0453680:intron 25.0%
! AACATGCTTTGATAGAATTA+TGG + chr3_3:44364846-44364865 Msa0453680:intron 25.0%
! AAAGGAAAGTCATTTGATAA+AGG + chr3_3:44364884-44364903 Msa0453680:CDS 25.0%
! AAATGTTAAGAACAAGTACA+AGG + chr3_3:44365046-44365065 Msa0453680:CDS 25.0%
!! TAGCAATTACACAAACTTTT+TGG - chr3_3:44365157-44365176 None:intergenic 25.0%
AATATTGAAGTTGGGAGAAT+GGG - chr3_3:44364039-44364058 None:intergenic 30.0%
AAATATTGAAGTTGGGAGAA+TGG - chr3_3:44364040-44364059 None:intergenic 30.0%
AAAGTAAGAATGATCCTAGT+AGG + chr3_3:44364329-44364348 Msa0453680:intron 30.0%
!! AGATTTGTTAGTTGTGTTTG+AGG + chr3_3:44364422-44364441 Msa0453680:intron 30.0%
TTTGTGAGGTGGAAAATTAA+AGG + chr3_3:44364502-44364521 Msa0453680:intron 30.0%
AATTACAAGTGTCTATGTGT+CGG - chr3_3:44364732-44364751 None:intergenic 30.0%
!!! TTTCACTTTTTGTGTTCCAT+TGG - chr3_3:44364961-44364980 None:intergenic 30.0%
AATATTGAAGTTGGGAGAAT+GGG - chr3_3:44364039-44364058 None:intergenic 30.0%
AAATATTGAAGTTGGGAGAA+TGG - chr3_3:44364040-44364059 None:intergenic 30.0%
AAAGTAAGAATGATCCTAGT+AGG + chr3_3:44364329-44364348 Msa0453680:intron 30.0%
!! AGATTTGTTAGTTGTGTTTG+AGG + chr3_3:44364422-44364441 Msa0453680:intron 30.0%
TTTGTGAGGTGGAAAATTAA+AGG + chr3_3:44364502-44364521 Msa0453680:intron 30.0%
AATTACAAGTGTCTATGTGT+CGG - chr3_3:44364732-44364751 None:intergenic 30.0%
!!! TTTCACTTTTTGTGTTCCAT+TGG - chr3_3:44364961-44364980 None:intergenic 30.0%
AACACTTCATGCAACCTAAA+CGG - chr3_3:44363964-44363983 None:intergenic 35.0%
ATATTGAAGTTGGGAGAATG+GGG - chr3_3:44364038-44364057 None:intergenic 35.0%
TTGTAGAAGAATGGTCTAGA+GGG - chr3_3:44364174-44364193 None:intergenic 35.0%
TTGATGGAGTTGTAGAAGAA+TGG - chr3_3:44364183-44364202 None:intergenic 35.0%
ATTCTTCTACAACTCCATCA+AGG + chr3_3:44364182-44364201 Msa0453680:CDS 35.0%
! AATTTTCCACCTCACAAAAC+AGG - chr3_3:44364500-44364519 None:intergenic 35.0%
GAATTATGGACAAGAAGTGA+AGG + chr3_3:44364860-44364879 Msa0453680:intron 35.0%
TGAAATTCTTCAGCTGTAGA+TGG - chr3_3:44364918-44364937 None:intergenic 35.0%
TGGAGACTTCATGAATCATT+AGG + chr3_3:44365322-44365341 Msa0453680:three_prime_UTR 35.0%
AACACTTCATGCAACCTAAA+CGG - chr3_3:44363964-44363983 None:intergenic 35.0%
ATATTGAAGTTGGGAGAATG+GGG - chr3_3:44364038-44364057 None:intergenic 35.0%
TTGTAGAAGAATGGTCTAGA+GGG - chr3_3:44364174-44364193 None:intergenic 35.0%
TTGATGGAGTTGTAGAAGAA+TGG - chr3_3:44364183-44364202 None:intergenic 35.0%
ATTCTTCTACAACTCCATCA+AGG + chr3_3:44364182-44364201 Msa0453680:CDS 35.0%
! AATTTTCCACCTCACAAAAC+AGG - chr3_3:44364500-44364519 None:intergenic 35.0%
GAATTATGGACAAGAAGTGA+AGG + chr3_3:44364860-44364879 Msa0453680:intron 35.0%
TGAAATTCTTCAGCTGTAGA+TGG - chr3_3:44364918-44364937 None:intergenic 35.0%
TGGAGACTTCATGAATCATT+AGG + chr3_3:44365322-44365341 Msa0453680:three_prime_UTR 35.0%
GTGAAGAAGAGTGAGGAATT+GGG - chr3_3:44364124-44364143 None:intergenic 40.0%
AGTGAAGAAGAGTGAGGAAT+TGG - chr3_3:44364125-44364144 None:intergenic 40.0%
AAGATGAGTGAAGAAGAGTG+AGG - chr3_3:44364131-44364150 None:intergenic 40.0%
GTTGTAGAAGAATGGTCTAG+AGG - chr3_3:44364175-44364194 None:intergenic 40.0%
CTTCAACTCAATCTCCTACT+AGG - chr3_3:44364346-44364365 None:intergenic 40.0%
TGGACAAGAAGTGAAGGAAA+AGG + chr3_3:44364866-44364885 Msa0453680:CDS 40.0%
AGAATTTCAGAGAGTAGCAG+AGG + chr3_3:44364929-44364948 Msa0453680:CDS 40.0%
AAGCTGCTAAGGGTAATTCA+AGG + chr3_3:44365018-44365037 Msa0453680:CDS 40.0%
!! GTACAAGGAGCATTGATCTT+AGG + chr3_3:44365061-44365080 Msa0453680:exon 40.0%
GTGAAGAAGAGTGAGGAATT+GGG - chr3_3:44364124-44364143 None:intergenic 40.0%
AGTGAAGAAGAGTGAGGAAT+TGG - chr3_3:44364125-44364144 None:intergenic 40.0%
AAGATGAGTGAAGAAGAGTG+AGG - chr3_3:44364131-44364150 None:intergenic 40.0%
GTTGTAGAAGAATGGTCTAG+AGG - chr3_3:44364175-44364194 None:intergenic 40.0%
CTTCAACTCAATCTCCTACT+AGG - chr3_3:44364346-44364365 None:intergenic 40.0%
TGGACAAGAAGTGAAGGAAA+AGG + chr3_3:44364866-44364885 Msa0453680:CDS 40.0%
AGAATTTCAGAGAGTAGCAG+AGG + chr3_3:44364929-44364948 Msa0453680:CDS 40.0%
AAGCTGCTAAGGGTAATTCA+AGG + chr3_3:44365018-44365037 Msa0453680:CDS 40.0%
!! GTACAAGGAGCATTGATCTT+AGG + chr3_3:44365061-44365080 Msa0453680:exon 40.0%
GTGTGCAAAGTTGTCCGTTT+AGG + chr3_3:44363947-44363966 Msa0453680:five_prime_UTR 45.0%
!! TACACGTGGTGTTGTTGAAG+TGG - chr3_3:44363987-44364006 None:intergenic 45.0%
AATGGGGATGTGTCTATAGG+TGG - chr3_3:44364022-44364041 None:intergenic 45.0%
GAGAATGGGGATGTGTCTAT+AGG - chr3_3:44364025-44364044 None:intergenic 45.0%
GTATGGTTACTCTCTCTCTG+AGG - chr3_3:44364090-44364109 None:intergenic 45.0%
GAGAGAGAGTAACCATACCA+TGG + chr3_3:44364092-44364111 Msa0453680:exon 45.0%
! CTGCATGATCCTGTTTTGTG+AGG + chr3_3:44364488-44364507 Msa0453680:intron 45.0%
! CATGATCCTGTTTTGTGAGG+TGG + chr3_3:44364491-44364510 Msa0453680:intron 45.0%
GTAGCAGAGGAGAAAACCAA+TGG + chr3_3:44364942-44364961 Msa0453680:CDS 45.0%
GTGTGCAAAGTTGTCCGTTT+AGG + chr3_3:44363947-44363966 Msa0453680:five_prime_UTR 45.0%
!! TACACGTGGTGTTGTTGAAG+TGG - chr3_3:44363987-44364006 None:intergenic 45.0%
AATGGGGATGTGTCTATAGG+TGG - chr3_3:44364022-44364041 None:intergenic 45.0%
GAGAATGGGGATGTGTCTAT+AGG - chr3_3:44364025-44364044 None:intergenic 45.0%
GTATGGTTACTCTCTCTCTG+AGG - chr3_3:44364090-44364109 None:intergenic 45.0%
GAGAGAGAGTAACCATACCA+TGG + chr3_3:44364092-44364111 Msa0453680:exon 45.0%
! CTGCATGATCCTGTTTTGTG+AGG + chr3_3:44364488-44364507 Msa0453680:intron 45.0%
! CATGATCCTGTTTTGTGAGG+TGG + chr3_3:44364491-44364510 Msa0453680:intron 45.0%
GTAGCAGAGGAGAAAACCAA+TGG + chr3_3:44364942-44364961 Msa0453680:CDS 45.0%
TGTAGACACCACCCTATGTG+AGG + chr3_3:44363919-44363938 Msa0453680:five_prime_UTR 50.0%
GTAGACACCACCCTATGTGA+GGG + chr3_3:44363920-44363939 Msa0453680:five_prime_UTR 50.0%
GGAAGCTAGTGATGTACACG+TGG - chr3_3:44364001-44364020 None:intergenic 50.0%
! TTACTCTCTCTCTGAGGAGG+AGG - chr3_3:44364084-44364103 None:intergenic 50.0%
! TGGTTACTCTCTCTCTGAGG+AGG - chr3_3:44364087-44364106 None:intergenic 50.0%
GAGAGTAACCATACCATGGC+TGG + chr3_3:44364096-44364115 Msa0453680:exon 50.0%
TGCTGCTGAAGAAGCTGCTA+AGG + chr3_3:44365007-44365026 Msa0453680:CDS 50.0%
GCTGCTGAAGAAGCTGCTAA+GGG + chr3_3:44365008-44365027 Msa0453680:CDS 50.0%
TGTAGACACCACCCTATGTG+AGG + chr3_3:44363919-44363938 Msa0453680:five_prime_UTR 50.0%
GTAGACACCACCCTATGTGA+GGG + chr3_3:44363920-44363939 Msa0453680:five_prime_UTR 50.0%
GGAAGCTAGTGATGTACACG+TGG - chr3_3:44364001-44364020 None:intergenic 50.0%
! TTACTCTCTCTCTGAGGAGG+AGG - chr3_3:44364084-44364103 None:intergenic 50.0%
! TGGTTACTCTCTCTCTGAGG+AGG - chr3_3:44364087-44364106 None:intergenic 50.0%
GAGAGTAACCATACCATGGC+TGG + chr3_3:44364096-44364115 Msa0453680:exon 50.0%
TGCTGCTGAAGAAGCTGCTA+AGG + chr3_3:44365007-44365026 Msa0453680:CDS 50.0%
GCTGCTGAAGAAGCTGCTAA+GGG + chr3_3:44365008-44365027 Msa0453680:CDS 50.0%
GCACACTGCACCCTCACATA+GGG - chr3_3:44363933-44363952 None:intergenic 55.0%
TGCACACTGCACCCTCACAT+AGG - chr3_3:44363934-44363953 None:intergenic 55.0%
ATTGGGCACCAGCCATGGTA+TGG - chr3_3:44364107-44364126 None:intergenic 55.0%
!! GTCTATGTGTCGGTGTCGTG+TGG - chr3_3:44364722-44364741 None:intergenic 55.0%
ACAGACACGAGACACGACAC+TGG - chr3_3:44364793-44364812 None:intergenic 55.0%
GCACACTGCACCCTCACATA+GGG - chr3_3:44363933-44363952 None:intergenic 55.0%
TGCACACTGCACCCTCACAT+AGG - chr3_3:44363934-44363953 None:intergenic 55.0%
ATTGGGCACCAGCCATGGTA+TGG - chr3_3:44364107-44364126 None:intergenic 55.0%
!! GTCTATGTGTCGGTGTCGTG+TGG - chr3_3:44364722-44364741 None:intergenic 55.0%
ACAGACACGAGACACGACAC+TGG - chr3_3:44364793-44364812 None:intergenic 55.0%
CACTGCACCCTCACATAGGG+TGG - chr3_3:44363930-44363949 None:intergenic 60.0%
GAGGAATTGGGCACCAGCCA+TGG - chr3_3:44364112-44364131 None:intergenic 60.0%
!! GTGTCGGTGTCGTGTGGTGT+CGG - chr3_3:44364716-44364735 None:intergenic 60.0%
CACTGCACCCTCACATAGGG+TGG - chr3_3:44363930-44363949 None:intergenic 60.0%
GAGGAATTGGGCACCAGCCA+TGG - chr3_3:44364112-44364131 None:intergenic 60.0%
!! GTGTCGGTGTCGTGTGGTGT+CGG - chr3_3:44364716-44364735 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 44363871 44365354 44363871 ID=Msa0453680;Name=Msa0453680
chr3_3 mRNA 44363871 44365354 44363871 ID=Msa0453680-mRNA-1;Parent=Msa0453680;Name=Msa0453680-mRNA-1;_AED=0.00;_eAED=0.00;_QI=240|1|1|1|1|1|2|278|102
chr3_3 exon 44363871 44364203 44363871 ID=Msa0453680-mRNA-1:exon:8129;Parent=Msa0453680-mRNA-1
chr3_3 exon 44364861 44365354 44364861 ID=Msa0453680-mRNA-1:exon:8130;Parent=Msa0453680-mRNA-1
chr3_3 five_prime_UTR 44363871 44364110 44363871 ID=Msa0453680-mRNA-1:five_prime_utr;Parent=Msa0453680-mRNA-1
chr3_3 CDS 44364111 44364203 44364111 ID=Msa0453680-mRNA-1:cds;Parent=Msa0453680-mRNA-1
chr3_3 CDS 44364861 44365076 44364861 ID=Msa0453680-mRNA-1:cds;Parent=Msa0453680-mRNA-1
chr3_3 three_prime_UTR 44365077 44365354 44365077 ID=Msa0453680-mRNA-1:three_prime_utr;Parent=Msa0453680-mRNA-1
Gene Sequence

>Msa0453680

ATGGCTGGTGCCCAATTCCTCACTCTTCTTCACTCATCTTCAAGATCTTCTTTGCTCAAACCCTCTAGACCATTCTTCTACAACTCCATCAAGAATTATGGACAAGAAGTGAAGGAAAAGGAAAGTCATTTGATAAAGGAGAGAGCACCATCTACAGCTGAAGAATTTCAGAGAGTAGCAGAGGAGAAAACCAATGGAACACAAAAAGTGAAAAGTCAAACAGTTGATAAAGCAATTGATGCTGCTGAAGAAGCTGCTAAGGGTAATTCAAGGATTGAAAATGTTAAGAACAAGTACAAGGAGCATTGA
Protein sequence

>Msa0453680

MAGAQFLTLLHSSSRSSLLKPSRPFFYNSIKNYGQEVKEKESHLIKERAPSTAEEFQRVAEEKTNGTQKVKSQTVDKAIDAAEEAAKGNSRIENVKNKYKEH*