AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0261550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261550 MtrunA17_Chr2g0323761 97.701 87 2 0 1 87 1 87 5.32e-61 180
Msa0261550 MtrunA17_Chr2g0323741 81.111 90 14 2 1 87 1 90 2.44e-48 148
Msa0261550 MtrunA17_Chr2g0323751 75.824 91 18 2 1 87 1 91 9.83e-46 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261550 AT4G28240.1 46.739 92 38 3 1 87 1 86 3.70e-20 77.4

Find 33 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGAGACGAGTTCGGTTT+TGG 0.305492 2_2:-60006259 None:intergenic
TCCGATCTCCGGCCGTTGTC+CGG 0.378956 2_2:+60006296 Msa0261550:CDS
AATTCATGTACATAACTTTC+CGG 0.379119 2_2:-60006382 None:intergenic
AAGTTATGTACATGAATTGC+TGG 0.397828 2_2:+60006387 Msa0261550:CDS
TGAGTTACTTGAACCGTGTT+TGG 0.412758 2_2:+60006159 Msa0261550:CDS
GCGCAAGGCCACACCGATCC+AGG 0.445476 2_2:+60006203 Msa0261550:CDS
CAACGACGCCGGACAACGGC+CGG 0.455353 2_2:-60006304 None:intergenic
CCGGAGATCGGATAAACTTC+CGG 0.462860 2_2:-60006285 None:intergenic
CGGCAGAGAAGAGACGAGTT+CGG 0.471245 2_2:-60006265 None:intergenic
GTTGTCCGGCGTCGTTGTAT+CGG 0.492908 2_2:+60006310 Msa0261550:CDS
GTGGCCTTGCGCCACCGCTA+CGG 0.494254 2_2:-60006192 None:intergenic
TTTGCACTTGTGGCCTGGAT+CGG 0.503366 2_2:-60006216 None:intergenic
GCCGGACAACGGCCGGAGAT+CGG 0.526728 2_2:-60006297 None:intergenic
AGTTATGTACATGAATTGCT+GGG 0.535583 2_2:+60006388 Msa0261550:CDS
CGAACTCGTCTCTTCTCTGC+CGG 0.543523 2_2:+60006266 Msa0261550:CDS
GCAACCGTAGCGGTGGCGCA+AGG 0.545121 2_2:+60006188 Msa0261550:CDS
AACTTTCCGGAGAGAATCAT+CGG 0.554058 2_2:-60006369 None:intergenic
CCGGAAGTTTATCCGATCTC+CGG 0.556561 2_2:+60006285 Msa0261550:CDS
GAAGAACCGATGATTCTCTC+CGG 0.569326 2_2:+60006363 Msa0261550:CDS
CTTGAACCGTGTTTGGATGG+CGG 0.571348 2_2:+60006166 Msa0261550:CDS
TTACTTGAACCGTGTTTGGA+TGG 0.584544 2_2:+60006163 Msa0261550:CDS
GATGGCGGCAACCGTAGCGG+TGG 0.585060 2_2:+60006181 Msa0261550:CDS
TACATGAATTGCTGGGGTCA+AGG 0.586623 2_2:+60006395 Msa0261550:CDS
GATACAACGACGCCGGACAA+CGG 0.587431 2_2:-60006308 None:intergenic
CACAAGTGCAAAACAGCCGT+TGG 0.609626 2_2:+60006227 Msa0261550:CDS
GTCCGGCGTCGTTGTATCGG+AGG 0.612227 2_2:+60006313 Msa0261550:CDS
CGGTTGCCGCCATCCAAACA+CGG 0.636549 2_2:-60006172 None:intergenic
TGTATCGGAGGTGAGTTCTG+AGG 0.650249 2_2:+60006325 Msa0261550:CDS
ATCGGAGGTGAGTTCTGAGG+CGG 0.654083 2_2:+60006328 Msa0261550:CDS
TTGGATGGCGGCAACCGTAG+CGG 0.657920 2_2:+60006178 Msa0261550:CDS
CACCTCCGATACAACGACGC+CGG 0.661000 2_2:-60006315 None:intergenic
ACTTGTGGCCTGGATCGGTG+TGG 0.680459 2_2:-60006211 None:intergenic
GTTATGTACATGAATTGCTG+GGG 0.680638 2_2:+60006389 Msa0261550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTCATGTACATAACTTTC+CGG - chr2_2:60006385-60006404 None:intergenic 25.0%
AAGTTATGTACATGAATTGC+TGG + chr2_2:60006387-60006406 Msa0261550:CDS 30.0%
AGTTATGTACATGAATTGCT+GGG + chr2_2:60006388-60006407 Msa0261550:CDS 30.0%
!! GTTTTGGTGAATAGATCCAA+CGG - chr2_2:60006246-60006265 None:intergenic 35.0%
GTTATGTACATGAATTGCTG+GGG + chr2_2:60006389-60006408 Msa0261550:CDS 35.0%
TGAGTTACTTGAACCGTGTT+TGG + chr2_2:60006159-60006178 Msa0261550:CDS 40.0%
TTACTTGAACCGTGTTTGGA+TGG + chr2_2:60006163-60006182 Msa0261550:CDS 40.0%
AACTTTCCGGAGAGAATCAT+CGG - chr2_2:60006372-60006391 None:intergenic 40.0%
! AGAAGAGACGAGTTCGGTTT+TGG - chr2_2:60006262-60006281 None:intergenic 45.0%
GAAGAACCGATGATTCTCTC+CGG + chr2_2:60006363-60006382 Msa0261550:CDS 45.0%
TACATGAATTGCTGGGGTCA+AGG + chr2_2:60006395-60006414 Msa0261550:CDS 45.0%
CTTGAACCGTGTTTGGATGG+CGG + chr2_2:60006166-60006185 Msa0261550:CDS 50.0%
TTTGCACTTGTGGCCTGGAT+CGG - chr2_2:60006219-60006238 None:intergenic 50.0%
!! CAACGGCTGTTTTGCACTTG+TGG - chr2_2:60006229-60006248 None:intergenic 50.0%
CACAAGTGCAAAACAGCCGT+TGG + chr2_2:60006227-60006246 Msa0261550:CDS 50.0%
CCGGAGATCGGATAAACTTC+CGG - chr2_2:60006288-60006307 None:intergenic 50.0%
CCGGAAGTTTATCCGATCTC+CGG + chr2_2:60006285-60006304 Msa0261550:CDS 50.0%
TGTATCGGAGGTGAGTTCTG+AGG + chr2_2:60006325-60006344 Msa0261550:CDS 50.0%
!! GCTGTTTTGCACTTGTGGCC+TGG - chr2_2:60006224-60006243 None:intergenic 55.0%
CGGCAGAGAAGAGACGAGTT+CGG - chr2_2:60006268-60006287 None:intergenic 55.0%
CGAACTCGTCTCTTCTCTGC+CGG + chr2_2:60006266-60006285 Msa0261550:CDS 55.0%
GATACAACGACGCCGGACAA+CGG - chr2_2:60006311-60006330 None:intergenic 55.0%
GTTGTCCGGCGTCGTTGTAT+CGG + chr2_2:60006310-60006329 Msa0261550:CDS 55.0%
!! ATCGGAGGTGAGTTCTGAGG+CGG + chr2_2:60006328-60006347 Msa0261550:CDS 55.0%
CGGTTGCCGCCATCCAAACA+CGG - chr2_2:60006175-60006194 None:intergenic 60.0%
! TTGGATGGCGGCAACCGTAG+CGG + chr2_2:60006178-60006197 Msa0261550:CDS 60.0%
ACTTGTGGCCTGGATCGGTG+TGG - chr2_2:60006214-60006233 None:intergenic 60.0%
CACCTCCGATACAACGACGC+CGG - chr2_2:60006318-60006337 None:intergenic 60.0%
TCCGATCTCCGGCCGTTGTC+CGG + chr2_2:60006296-60006315 Msa0261550:CDS 65.0%
GTCCGGCGTCGTTGTATCGG+AGG + chr2_2:60006313-60006332 Msa0261550:CDS 65.0%
! GATGGCGGCAACCGTAGCGG+TGG + chr2_2:60006181-60006200 Msa0261550:CDS 70.0%
GCAACCGTAGCGGTGGCGCA+AGG + chr2_2:60006188-60006207 Msa0261550:CDS 70.0%
! GTGGCCTTGCGCCACCGCTA+CGG - chr2_2:60006195-60006214 None:intergenic 70.0%
GCGCAAGGCCACACCGATCC+AGG + chr2_2:60006203-60006222 Msa0261550:CDS 70.0%
GCCGGACAACGGCCGGAGAT+CGG - chr2_2:60006300-60006319 None:intergenic 70.0%
CAACGACGCCGGACAACGGC+CGG - chr2_2:60006307-60006326 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 60006158 60006421 60006158 ID=Msa0261550;Name=Msa0261550
chr2_2 mRNA 60006158 60006421 60006158 ID=Msa0261550-mRNA-1;Parent=Msa0261550;Name=Msa0261550-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|87
chr2_2 exon 60006158 60006421 60006158 ID=Msa0261550-mRNA-1:exon:18474;Parent=Msa0261550-mRNA-1
chr2_2 CDS 60006158 60006421 60006158 ID=Msa0261550-mRNA-1:cds;Parent=Msa0261550-mRNA-1
Gene Sequence

>Msa0261550

ATGAGTTACTTGAACCGTGTTTGGATGGCGGCAACCGTAGCGGTGGCGCAAGGCCACACCGATCCAGGCCACAAGTGCAAAACAGCCGTTGGATCTATTCACCAAAACCGAACTCGTCTCTTCTCTGCCGGAAGTTTATCCGATCTCCGGCCGTTGTCCGGCGTCGTTGTATCGGAGGTGAGTTCTGAGGCGGATGAGAAGTTAAGAAGAACCGATGATTCTCTCCGGAAAGTTATGTACATGAATTGCTGGGGTCAAGGCTGA

Protein sequence

>Msa0261550

MSYLNRVWMAATVAVAQGHTDPGHKCKTAVGSIHQNRTRLFSAGSLSDLRPLSGVVVSEVSSEADEKLRRTDDSLRKVMYMNCWGQG*