AlfalfaGEDB | Msa0227100

M. sativa cultivar zhongmu-4 / Msa0227100

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227100	XP_003597966.1	89.274	317	29	4	36	351	1	313	0.0	565

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227100	sp\|Q9T072\|BH025_ARATH	38.462	221	120	5	132	346	116	326	5.47e-37	139

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227100	G7IMA7	89.274	317	29	4	36	351	1	313	0.0	565

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TFs/TRs:

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Gene ID	Type	Classification
Msa0227100	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0026120	Msa0227100	0.817390	3.380256e-52	-8.615850e-47
Msa0050850	Msa0227100	0.812906	3.348418e-51	-8.615850e-47
Msa0082250	Msa0227100	0.819275	1.264814e-52	-8.615850e-47
Msa0094060	Msa0227100	0.818092	2.347672e-52	-8.615850e-47
Msa0112800	Msa0227100	0.817082	3.964798e-52	-8.615850e-47
Msa0114960	Msa0227100	0.814486	1.503792e-51	-8.615850e-47
Msa0155500	Msa0227100	0.800591	1.341277e-48	-8.615850e-47
Msa0166580	Msa0227100	0.816377	5.707953e-52	-8.615850e-47
Msa0207220	Msa0227100	0.811816	5.792428e-51	-8.615850e-47
Msa0210870	Msa0227100	0.829457	5.092053e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227100	MtrunA17_Chr2g0333641	89.274	317	29	4	36	351	1	313	0.0	565
Msa0227100	MtrunA17_Chr2g0333621	78.730	315	56	6	36	344	1	310	4.80e-165	463
Msa0227100	MtrunA17_Chr4g0002801	43.143	350	137	10	36	344	1	329	1.98e-74	233
Msa0227100	MtrunA17_Chr4g0002781	42.458	358	147	10	36	344	1	348	2.78e-69	220
Msa0227100	MtrunA17_Chr8g0336681	47.581	248	116	6	111	349	97	339	1.73e-56	187
Msa0227100	MtrunA17_Chr7g0249811	37.278	338	172	6	36	344	1	327	4.57e-53	178
Msa0227100	MtrunA17_Chr5g0400621	43.096	239	106	5	116	340	102	324	2.04e-45	158
Msa0227100	MtrunA17_Chr5g0400661	38.111	307	141	11	76	344	38	333	2.40e-43	152
Msa0227100	MtrunA17_Chr6g0468491	41.026	273	135	9	79	340	47	304	8.38e-43	150
Msa0227100	MtrunA17_Chr2g0307131	41.475	217	118	5	132	340	131	346	1.91e-42	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227100	AT4G37850.2	38.462	221	120	5	132	346	116	326	5.56e-38	139
Msa0227100	AT4G37850.1	38.462	221	120	5	132	346	116	326	5.56e-38	139
Msa0227100	AT2G22750.2	36.546	249	138	6	112	348	65	305	9.79e-33	124
Msa0227100	AT2G22750.3	36.546	249	138	6	112	348	93	333	1.27e-32	124
Msa0227100	AT2G22750.5	36.546	249	138	6	112	348	89	329	1.35e-32	124
Msa0227100	AT2G22760.1	41.667	180	99	3	167	340	111	290	2.95e-32	122
Msa0227100	AT2G22750.1	36.546	249	137	7	112	348	65	304	2.33e-31	120
Msa0227100	AT2G22750.4	36.546	249	137	7	112	348	93	332	3.50e-31	120
Msa0227100	AT2G22760.2	42.073	164	90	2	167	325	111	274	4.42e-28	111
Msa0227100	AT2G22770.1	35.638	188	104	2	175	345	132	319	4.83e-25	103

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Find 70 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATAGAATAAATGTCCT+TGG	0.468384	2_1:+77704686	None:intergenic
AAGCTAATGGAATGGGGATA+AGG	0.375867	2_1:+77705112	None:intergenic
AAGTAATAATAAGAGGAGTT+TGG	0.391916	2_1:-77704582	Msa0227100:CDS
AAGTAATTAATGTTTGATCG+TGG	0.592571	2_1:-77705174	Msa0227100:exon
AATATGTAACAGGTTGCTGA+AGG	0.616197	2_1:+77704795	None:intergenic
ACTACTTCAAGAACGTGTGA+AGG	0.653803	2_1:-77704132	Msa0227100:CDS
ACTGGATGAAGCTAATGGAA+TGG	0.547841	2_1:+77705104	None:intergenic
ACTTCTTGTTCTGATTCTTC+TGG	0.307827	2_1:+77704623	None:intergenic
AGAACATCATGGAGGAATCA+TGG	0.464890	2_1:-77705068	Msa0227100:CDS
AGAACGTGTGAAGGAGCTAG+AGG	0.643700	2_1:-77704123	Msa0227100:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAATGGTGACAATAGGAAT+TGG	-	chr2_1:77703130-77703149	Msa0227100:intron	30.0%
TAATGGTGACAATAGGAATT+GGG	-	chr2_1:77703131-77703150	Msa0227100:intron	30.0%
TTACTTCTACTCTTTGTTCT+TGG	+	chr2_1:77703428-77703447	None:intergenic	30.0%
AGAAGAACATATTAATCCCT+CGG	+	chr2_1:77703740-77703759	None:intergenic	30.0%
ATGGACAAGAACTATATACT+TGG	-	chr2_1:77703848-77703867	Msa0227100:CDS	30.0%
AGCAAGAATATTGGAAAATG+AGG	-	chr2_1:77704045-77704064	Msa0227100:CDS	30.0%
TTAATGGTGACAATAGGAAT+TGG	-	chr2_1:77703130-77703149	Msa0227100:intron	30.0%
TAATGGTGACAATAGGAATT+GGG	-	chr2_1:77703131-77703150	Msa0227100:intron	30.0%
TTACTTCTACTCTTTGTTCT+TGG	+	chr2_1:77703428-77703447	None:intergenic	30.0%
AGAAGAACATATTAATCCCT+CGG	+	chr2_1:77703740-77703759	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	77702830	77705216	77702830	ID=Msa0227100;Name=Msa0227100
chr2_1	mRNA	77702830	77705216	77702830	ID=Msa0227100-mRNA-1;Parent=Msa0227100;Name=Msa0227100-mRNA-1;_AED=0.15;_eAED=0.15;_QI=29\|1\|0.75\|1\|1\|1\|4\|141\|351
chr2_1	exon	77702830	77703054	77702830	ID=Msa0227100-mRNA-1:exon:23691;Parent=Msa0227100-mRNA-1
chr2_1	exon	77703836	77704198	77703836	ID=Msa0227100-mRNA-1:exon:23690;Parent=Msa0227100-mRNA-1
chr2_1	exon	77704433	77704900	77704433	ID=Msa0227100-mRNA-1:exon:23689;Parent=Msa0227100-mRNA-1
chr2_1	exon	77705047	77705216	77705047	ID=Msa0227100-mRNA-1:exon:23688;Parent=Msa0227100-mRNA-1
chr2_1	five_prime_UTR	77705188	77705216	77705188	ID=Msa0227100-mRNA-1:five_prime_utr;Parent=Msa0227100-mRNA-1
chr2_1	CDS	77705047	77705187	77705047	ID=Msa0227100-mRNA-1:cds;Parent=Msa0227100-mRNA-1
chr2_1	CDS	77704433	77704900	77704433	ID=Msa0227100-mRNA-1:cds;Parent=Msa0227100-mRNA-1
chr2_1	CDS	77703836	77704198	77703836	ID=Msa0227100-mRNA-1:cds;Parent=Msa0227100-mRNA-1
chr2_1	CDS	77702971	77703054	77702971	ID=Msa0227100-mRNA-1:cds;Parent=Msa0227100-mRNA-1
chr2_1	three_prime_UTR	77702830	77702970	77702830	ID=Msa0227100-mRNA-1:three_prime_utr;Parent=Msa0227100-mRNA-1

Gene Sequence

>Msa0227100

ATGTTTGATCGTGGACATTCTCAAATCTTGTCTGTACTTTCTTCTCCATCCTCCCTTATCCCCATTCCATTAGCTTCATCCAGTGAATACACATCACAGAACATCATGGAGGAATCATGGGAACATTGGCCTCTTCATTCGGAATTGGGTGATGTTGAGGATTTGCTAGGGCAATACTGTAAAACTAGTGCTGATGATGAAGAAGAGTTCTTGAGAGACATAATCCTTCAGCAACCTGTTACATATTCTGGAAGCAGTCTTTCATCCTCTAATGAGATGGATGGCTCAGATTGCTCAGGGAAAAAGCTCCATCCTTCATCAACACCGTCCACTCCAAGGACATTTATTCTATCTTTTGATAAATCAACCATAATACCAGCTACTACTACTACTACACCAGAAGAATCAGAACAAGAAGTACCAAGAACAAAGAGTAGAAGTAATAATAAGAGGAGTTTGGAACCAAAAGCAAAAGCAAGTAACCAAAGTGGAAAGAAGAGTAGAAGTGGTTCTCAGTGTTTGGATCACATTATGGCTGAGAGAAAAAGAAGACTAGAACTATCACAGAAGTTCATTGCACTTTCAGCTACTATTCCTGGCTTGAAGAAGATGGACAAGAACTATATACTTGGTGAAGCAATCAGTTATGTGAAACTACTTCAAGAACGTGTGAAGGAGCTAGAGGATCCAAACAAGAATAGCAAAGAATCAACAATAATTCTCAAGAAAACTGATATGTGTGTAAGTGAAGATACCACCTCAAATTCTGATCAGGATTGCTGCAAATCACCACTCTTTGATGTTAAAGCAAGAATATTGGAAAATGAGGTACTCATTCAGATCCACTGTGAGAAGGAAATTGACATTGAGATCAAAATATATAACGTGCTTCAAAATCTTGATCTCTTTGTCACTGCTAGCAGTGTATTGGCTTTTGGAACTTCAACTCTTGGCTTTACTATTGTCGCTCAGATGGGTGAAGGGTACGAAATGACGGTGATTGATTTGGTGAAAACTCTATCGCATGTGCTGACTAGCAGAACTGATCCGTTCTAG

Protein sequence

>Msa0227100

MFDRGHSQILSVLSSPSSLIPIPLASSSEYTSQNIMEESWEHWPLHSELGDVEDLLGQYCKTSADDEEEFLRDIILQQPVTYSGSSLSSSNEMDGSDCSGKKLHPSSTPSTPRTFILSFDKSTIIPATTTTTPEESEQEVPRTKSRSNNKRSLEPKAKASNQSGKKSRSGSQCLDHIMAERKRRLELSQKFIALSATIPGLKKMDKNYILGEAISYVKLLQERVKELEDPNKNSKESTIILKKTDMCVSEDTTSNSDQDCCKSPLFDVKARILENEVLIQIHCEKEIDIEIKIYNVLQNLDLFVTASSVLAFGTSTLGFTIVAQMGEGYEMTVIDLVKTLSHVLTSRTDPF*