Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762470 | sp|Q8RY46|AB26B_ARATH | 68.110 | 693 | 171 | 3 | 115 | 768 | 18 | 699 | 0.0 | 953 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000180 | Msa0762470 | 0.829050 | 6.393999e-55 | -8.615850e-47 |
| Msa0000220 | Msa0762470 | 0.867566 | 1.245032e-65 | -8.615850e-47 |
| Msa0000610 | Msa0762470 | 0.820001 | 8.636614e-53 | -8.615850e-47 |
| Msa0001040 | Msa0762470 | 0.857360 | 1.721433e-62 | -8.615850e-47 |
| Msa0001510 | Msa0762470 | 0.849559 | 2.992343e-60 | -8.615850e-47 |
| Msa0001540 | Msa0762470 | 0.809652 | 1.702498e-50 | -8.615850e-47 |
| Msa0002690 | Msa0762470 | 0.889979 | 1.490235e-73 | -8.615850e-47 |
| Msa0002700 | Msa0762470 | 0.896018 | 5.513834e-76 | -8.615850e-47 |
| Msa0003340 | Msa0762470 | 0.813553 | 2.414310e-51 | -8.615850e-47 |
| Msa0003370 | Msa0762470 | 0.847057 | 1.470801e-59 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762470 | MtrunA17_Chr5g0412741 | 91.725 | 713 | 15 | 4 | 89 | 769 | 1 | 701 | 0.0 | 1307 |
| Msa0762470 | MtrunA17_Chr7g0227391 | 56.847 | 628 | 229 | 4 | 170 | 768 | 54 | 668 | 0.0 | 729 |
| Msa0762470 | MtrunA17_Chr4g0061601 | 38.812 | 505 | 278 | 5 | 266 | 761 | 140 | 622 | 1.61e-102 | 329 |
| Msa0762470 | MtrunA17_Chr4g0069361 | 34.630 | 514 | 288 | 12 | 266 | 760 | 786 | 1270 | 5.72e-75 | 265 |
| Msa0762470 | MtrunA17_Chr4g0069361 | 35.294 | 510 | 293 | 12 | 268 | 764 | 131 | 616 | 5.35e-73 | 259 |
| Msa0762470 | MtrunA17_Chr6g0481921 | 35.632 | 522 | 285 | 12 | 268 | 769 | 24 | 514 | 1.91e-74 | 263 |
| Msa0762470 | MtrunA17_Chr6g0481921 | 34.496 | 516 | 287 | 13 | 266 | 760 | 677 | 1162 | 1.33e-72 | 257 |
| Msa0762470 | MtrunA17_Chr6g0451911 | 35.458 | 502 | 285 | 11 | 267 | 753 | 765 | 1242 | 3.42e-74 | 263 |
| Msa0762470 | MtrunA17_Chr6g0451911 | 33.794 | 506 | 306 | 10 | 268 | 764 | 114 | 599 | 7.91e-68 | 244 |
| Msa0762470 | MtrunA17_Chr4g0041871 | 34.791 | 526 | 290 | 12 | 268 | 769 | 93 | 589 | 2.13e-73 | 260 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762470 | AT1G70610.1 | 68.110 | 693 | 171 | 3 | 115 | 768 | 18 | 699 | 0.0 | 953 |
| Msa0762470 | AT1G70610.2 | 78.529 | 503 | 97 | 1 | 266 | 768 | 27 | 518 | 0.0 | 820 |
| Msa0762470 | AT5G39040.1 | 35.529 | 501 | 306 | 3 | 266 | 764 | 149 | 634 | 2.78e-89 | 294 |
| Msa0762470 | AT1G27940.1 | 37.549 | 506 | 286 | 11 | 268 | 764 | 124 | 608 | 2.64e-82 | 286 |
| Msa0762470 | AT1G27940.1 | 32.014 | 556 | 317 | 13 | 232 | 764 | 725 | 1242 | 1.12e-68 | 246 |
| Msa0762470 | AT1G28010.1 | 38.340 | 506 | 282 | 12 | 268 | 764 | 125 | 609 | 2.29e-80 | 281 |
| Msa0762470 | AT1G28010.1 | 33.792 | 509 | 303 | 9 | 268 | 764 | 758 | 1244 | 2.88e-67 | 243 |
| Msa0762470 | AT4G01830.1 | 35.968 | 506 | 295 | 10 | 268 | 764 | 106 | 591 | 6.46e-75 | 265 |
| Msa0762470 | AT4G01830.1 | 33.922 | 510 | 303 | 12 | 266 | 762 | 735 | 1223 | 1.13e-66 | 241 |
| Msa0762470 | AT2G47000.6 | 34.320 | 507 | 304 | 9 | 266 | 762 | 792 | 1279 | 8.72e-75 | 265 |
Find 199 sgRNAs with CRISPR-Local
Find 689 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCATACCAAACGGTAAC+GGG | 0.454848 | 5_2:-20642157 | None:intergenic |
| AAAGCGCTGCATATGGATTT+TGG | 0.200298 | 5_2:+20645281 | Msa0762470:CDS |
| AAATCAGGAGGATATTGAGT+GGG | 0.636760 | 5_2:+20647999 | Msa0762470:CDS |
| AACGAGGAAGAAGATGATGA+TGG | 0.504680 | 5_2:-20641769 | None:intergenic |
| AACTCTACCTGACCACTTGT+AGG | 0.450787 | 5_2:-20646421 | None:intergenic |
| AAGAAACCTTTGAAAAGTGT+AGG | 0.564388 | 5_2:-20639984 | None:intergenic |
| AAGAAACTCGTTAGAAGCAT+TGG | 0.448929 | 5_2:-20642035 | None:intergenic |
| AAGAAGAGAGGGTGAAATTG+TGG | 0.535051 | 5_2:-20641928 | None:intergenic |
| AAGACTTGGATGTCATGTGG+TGG | 0.696777 | 5_2:+20647804 | Msa0762470:CDS |
| AAGAGAGGGTGAAATTGTGG+CGG | 0.653474 | 5_2:-20641925 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTGTATTTGTATAAGGGATT+TGG | + | chr5_2:20639788-20639807 | Msa0762470:CDS | 30.0% | |
| GTATTTGTATAAGGGATTTG+GGG | + | chr5_2:20639790-20639809 | Msa0762470:CDS | 30.0% | |
| TGAATATTGGGACAAATAAG+AGG | + | chr5_2:20640101-20640120 | Msa0762470:intron | 30.0% | |
| ATAAACAGCTAAAACCAAGA+AGG | - | chr5_2:20640195-20640214 | None:intergenic | 30.0% | |
| ACACTAGTAAGATAACTAAG+AGG | + | chr5_2:20640308-20640327 | Msa0762470:intron | 30.0% | |
| CACTAGTAAGATAACTAAGA+GGG | + | chr5_2:20640309-20640328 | Msa0762470:intron | 30.0% | |
| ACCATAACAACACATAATTC+TGG | - | chr5_2:20640671-20640690 | None:intergenic | 30.0% | |
| CAAAAAAAATATATCCTGGC+GGG | - | chr5_2:20640737-20640756 | None:intergenic | 30.0% | |
| CAAAAAAAATATATCCTGGC+GGG | - | chr5_2:20640737-20640756 | None:intergenic | 30.0% | |
| TGTATTTATAGAGAAGTTGG+GGG | + | chr5_2:20640960-20640979 | Msa0762470:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 20639770 | 20649700 | 20639770 | ID=Msa0762470;Name=Msa0762470 |
| chr5_2 | mRNA | 20639770 | 20649700 | 20639770 | ID=Msa0762470-mRNA-1;Parent=Msa0762470;Name=Msa0762470-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0.73|0.75|0.85|1|1|20|524|769 |
| chr5_2 | exon | 20639770 | 20639859 | 20639770 | ID=Msa0762470-mRNA-1:exon:8694;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20639951 | 20639999 | 20639951 | ID=Msa0762470-mRNA-1:exon:8695;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20641684 | 20642255 | 20641684 | ID=Msa0762470-mRNA-1:exon:8696;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20642350 | 20642433 | 20642350 | ID=Msa0762470-mRNA-1:exon:8697;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20644240 | 20644284 | 20644240 | ID=Msa0762470-mRNA-1:exon:8698;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20644386 | 20644508 | 20644386 | ID=Msa0762470-mRNA-1:exon:8699;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20644593 | 20644696 | 20644593 | ID=Msa0762470-mRNA-1:exon:8700;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20644925 | 20644979 | 20644925 | ID=Msa0762470-mRNA-1:exon:8701;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20645070 | 20645140 | 20645070 | ID=Msa0762470-mRNA-1:exon:8702;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20645237 | 20645339 | 20645237 | ID=Msa0762470-mRNA-1:exon:8703;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20645520 | 20645730 | 20645520 | ID=Msa0762470-mRNA-1:exon:8704;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20646016 | 20646089 | 20646016 | ID=Msa0762470-mRNA-1:exon:8705;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20646215 | 20646271 | 20646215 | ID=Msa0762470-mRNA-1:exon:8706;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20646361 | 20646435 | 20646361 | ID=Msa0762470-mRNA-1:exon:8707;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20647493 | 20647525 | 20647493 | ID=Msa0762470-mRNA-1:exon:8708;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20647779 | 20647853 | 20647779 | ID=Msa0762470-mRNA-1:exon:8709;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20647937 | 20648218 | 20647937 | ID=Msa0762470-mRNA-1:exon:8710;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20648507 | 20648571 | 20648507 | ID=Msa0762470-mRNA-1:exon:8711;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20648647 | 20648707 | 20648647 | ID=Msa0762470-mRNA-1:exon:8712;Parent=Msa0762470-mRNA-1 |
| chr5_2 | exon | 20649096 | 20649700 | 20649096 | ID=Msa0762470-mRNA-1:exon:8713;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20639770 | 20639859 | 20639770 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20639951 | 20639999 | 20639951 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20641684 | 20642255 | 20641684 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20642350 | 20642433 | 20642350 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20644240 | 20644284 | 20644240 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20644386 | 20644508 | 20644386 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20644593 | 20644696 | 20644593 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20644925 | 20644979 | 20644925 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20645070 | 20645140 | 20645070 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20645237 | 20645339 | 20645237 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20645520 | 20645730 | 20645520 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20646016 | 20646089 | 20646016 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20646215 | 20646271 | 20646215 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20646361 | 20646435 | 20646361 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20647493 | 20647525 | 20647493 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20647779 | 20647853 | 20647779 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20647937 | 20648218 | 20647937 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20648507 | 20648571 | 20648507 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20648647 | 20648707 | 20648647 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | CDS | 20649096 | 20649176 | 20649096 | ID=Msa0762470-mRNA-1:cds;Parent=Msa0762470-mRNA-1 |
| chr5_2 | three_prime_UTR | 20649177 | 20649700 | 20649177 | ID=Msa0762470-mRNA-1:three_prime_utr;Parent=Msa0762470-mRNA-1 |
| Gene Sequence |
>Msa0762470 ATGAAGATTAGGGTTTTGGTGTATTTGTATAAGGGATTTGGGGAGGTTTACGTTTTTGTCCTTAGGGTTTGTGCGTTTTCAGACATGGAGGGGATGAGTCAATTGATAATTACTCTTCGGACCCCTACACTTTTCAAAGGAGAAAGAACAACTTCCATTGCCGCTTCTAATACACATGAACCACATAACACTCTTCTCAAACTTCTACCTTTCATCTCAAAAACCCATCATCATCTTCTTCCTCGTTACTTCGCTACCACCACCATGCCTCCACCACTCACCGGCACACATCACTATCATTACTTACTTCCTCCTTCAACCTCTCACCACCGCCATCAACGCCACCGCCACCAACCTCTAATCACATTCTCCTCCGTCAACCGCCACAATTTCACCCTCTCTTCTTCCAAACGCATTCTCTTATCCTCTTCAATAAAATCAGCTTCCATCAATGAAGTTTCAATCCACAACAGTTCAGAATCAGCAGCTTCCAATGCTTCTAACGAGTTTCTTGATAGAATACGCAAATGGGTTTCTTTCCTTCCTTCTATTTTCCCTGGAGGAACATGGTGGAACTTCTCCGATGACGTCGAGGTTTCCATGTTCAATCAACCCGTTACCGTTTGGTATGCTTTGGGTAAAATGTGGAACCTTGTGGCGAAAGATAGATGGGTTATTTTCGCTGCTTTTTCTGCTCTTATCATTGCAGCAGTTTCGGAGATTTCTATACCACATTTTTTGACAGCATCGATATTCTCGGCGCAAAGTGGTGATATTAAAGTTTTTCATGGGAATGTTAAGAGAATGAGAGAAACATTATACTCTTCCCTTCTTCTTCAGGATATATCATTTTTTGACAATGAAACAGTAGGTGATTTGACAAGTAGACTTGGGGCAGATTGTCAGCAAGTTTCAAGGGTTATTGGAAATGATCTTAATTTGATACTGCGCAATGTCCTTCAGGGAGGAGGTTCATTGATCTACTTGTTGATTTTGTCTTGGCCACTTGGTTTATGTACGCTGGTGATATGCTCCATATTAGCAGCGGTCATGTTACGTTATGGATGGTACCAGAAGAAGGCAGCAAGGTTGATCCAAGAAGTCACTGCTTCTGCGAATAATGTAGCTCAAGAGACATTCTCTCTTATTAGAACTGTTCGAGTTTATGGAACCGAAGAAGAAGAACACGGAAGGTACAAATGGTGGCTTGAAAAATTAGCTGACATAAGCTTGCGACAAAGCGCTGCATATGGATTTTGGAATTTCAGCTTCAACACTCTTTATCATTCAACTCAGATTATTGCTGTGCTGTTTGGAGGAATGTCTATTCTATCGGGTCATATTACAGCAGAGAAACTTACTAAGTTTATACTATATAGTGAATGGTTAATTTATTCAACCTGGTGGGTGGGGGATAATGTATCCAATTTAATGCAATCAGTTGGAGCTAGTGAAAAGGTGTTCAACTTGATGGATCTCTCACCTAGCAGCCAATTTATAACAGAAGGAATGAAGTTGCAGAGTTTAACGGGGCATATTGAGTTTGTAAATGTATCTTTTCACTATCCTTCAAGACCAACGGTACACGTAGTGCAACGTGTGAACTTTGTTGTCAATCCTAGTGAGGTGGTTGCCATTGTTGGTCTTAGTGGTAGCGGGAAAAGCACATTGGTGAATCTATTACTCCGTCTCTACGAGCCTACAAGTGGTCAGGTGCTTATACCTTCTATTCTAGATTCTTTACTGATTTTGATAGACGGTGTCCCTCACAAAGACTTGGATGTCATGTGGTGGAGAGAGAGGATCGGATATGTGGGACAGGAACCGAAGTTATTCCGGATGGATATTAGCTCGAACATCAGATATGGATGTACCCGTGATGTAAATCAGGAGGATATTGAGTGGGCTGCAAAACAGGCCTACGCTCATGACTTTATCTCAGCACTTCCTAGTGGTTATGAAACACTTGTTGATGACGATCTTTTAAGTGGCGGACAAAAGCAACGAATTGCTATCGCTAGGGCCATTCTTAGGGACCCGAAAATATTGATTCTTGATGAAGCCACTAGTGCACTGGATGCTGAAAGTGAACATAATGTGAAGGGTGTTCTTCGATCGGTTAGAAGTGATTCTTCATCAAGGAGAAGTGTCATAGTCATTGCACACAGGCTTTCTACCATACAAGCTGCTGACAGGATTATTGTGATGGATAAGGGTCAAATAGTTGAGAATGGAAGTCACAGGGAACTTCTCTTGAAAGATGGTTTATATGCTCGATTGACTAGAAAACAGGCTGATACCATGGAATGA |
| Protein sequence |
>Msa0762470 MKIRVLVYLYKGFGEVYVFVLRVCAFSDMEGMSQLIITLRTPTLFKGERTTSIAASNTHEPHNTLLKLLPFISKTHHHLLPRYFATTTMPPPLTGTHHYHYLLPPSTSHHRHQRHRHQPLITFSSVNRHNFTLSSSKRILLSSSIKSASINEVSIHNSSESAASNASNEFLDRIRKWVSFLPSIFPGGTWWNFSDDVEVSMFNQPVTVWYALGKMWNLVAKDRWVIFAAFSALIIAAVSEISIPHFLTASIFSAQSGDIKVFHGNVKRMRETLYSSLLLQDISFFDNETVGDLTSRLGADCQQVSRVIGNDLNLILRNVLQGGGSLIYLLILSWPLGLCTLVICSILAAVMLRYGWYQKKAARLIQEVTASANNVAQETFSLIRTVRVYGTEEEEHGRYKWWLEKLADISLRQSAAYGFWNFSFNTLYHSTQIIAVLFGGMSILSGHITAEKLTKFILYSEWLIYSTWWVGDNVSNLMQSVGASEKVFNLMDLSPSSQFITEGMKLQSLTGHIEFVNVSFHYPSRPTVHVVQRVNFVVNPSEVVAIVGLSGSGKSTLVNLLLRLYEPTSGQVLIPSILDSLLILIDGVPHKDLDVMWWRERIGYVGQEPKLFRMDISSNIRYGCTRDVNQEDIEWAAKQAYAHDFISALPSGYETLVDDDLLSGGQKQRIAIARAILRDPKILILDEATSALDAESEHNVKGVLRSVRSDSSSRRSVIVIAHRLSTIQAADRIIVMDKGQIVENGSHRELLLKDGLYARLTRKQADTME* |
