Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762390 | sp|Q8RY46|AB26B_ARATH | 68.110 | 693 | 171 | 3 | 115 | 768 | 18 | 699 | 0.0 | 953 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000180 | Msa0762390 | 0.827012 | 1.979179e-54 | -8.615850e-47 |
| Msa0000220 | Msa0762390 | 0.869148 | 3.847490e-66 | -8.615850e-47 |
| Msa0000610 | Msa0762390 | 0.823905 | 1.076803e-53 | -8.615850e-47 |
| Msa0001040 | Msa0762390 | 0.860524 | 1.945588e-63 | -8.615850e-47 |
| Msa0001510 | Msa0762390 | 0.851289 | 9.775908e-61 | -8.615850e-47 |
| Msa0001540 | Msa0762390 | 0.803703 | 3.071141e-49 | -8.615850e-47 |
| Msa0002110 | Msa0762390 | 0.803412 | 3.528862e-49 | -8.615850e-47 |
| Msa0002690 | Msa0762390 | 0.894610 | 2.097894e-75 | -8.615850e-47 |
| Msa0002700 | Msa0762390 | 0.900047 | 1.082560e-77 | -8.615850e-47 |
| Msa0003340 | Msa0762390 | 0.819693 | 1.015550e-52 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762390 | MtrunA17_Chr5g0412741 | 91.725 | 713 | 15 | 4 | 89 | 769 | 1 | 701 | 0.0 | 1307 |
| Msa0762390 | MtrunA17_Chr7g0227391 | 56.847 | 628 | 229 | 4 | 170 | 768 | 54 | 668 | 0.0 | 729 |
| Msa0762390 | MtrunA17_Chr4g0061601 | 38.812 | 505 | 278 | 5 | 266 | 761 | 140 | 622 | 1.61e-102 | 329 |
| Msa0762390 | MtrunA17_Chr4g0069361 | 34.630 | 514 | 288 | 12 | 266 | 760 | 786 | 1270 | 5.72e-75 | 265 |
| Msa0762390 | MtrunA17_Chr4g0069361 | 35.294 | 510 | 293 | 12 | 268 | 764 | 131 | 616 | 5.35e-73 | 259 |
| Msa0762390 | MtrunA17_Chr6g0481921 | 35.632 | 522 | 285 | 12 | 268 | 769 | 24 | 514 | 1.91e-74 | 263 |
| Msa0762390 | MtrunA17_Chr6g0481921 | 34.496 | 516 | 287 | 13 | 266 | 760 | 677 | 1162 | 1.33e-72 | 257 |
| Msa0762390 | MtrunA17_Chr6g0451911 | 35.458 | 502 | 285 | 11 | 267 | 753 | 765 | 1242 | 3.42e-74 | 263 |
| Msa0762390 | MtrunA17_Chr6g0451911 | 33.794 | 506 | 306 | 10 | 268 | 764 | 114 | 599 | 7.91e-68 | 244 |
| Msa0762390 | MtrunA17_Chr4g0041871 | 34.791 | 526 | 290 | 12 | 268 | 769 | 93 | 589 | 2.13e-73 | 260 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0762390 | AT1G70610.1 | 68.110 | 693 | 171 | 3 | 115 | 768 | 18 | 699 | 0.0 | 953 |
| Msa0762390 | AT1G70610.2 | 78.529 | 503 | 97 | 1 | 266 | 768 | 27 | 518 | 0.0 | 820 |
| Msa0762390 | AT5G39040.1 | 35.529 | 501 | 306 | 3 | 266 | 764 | 149 | 634 | 2.78e-89 | 294 |
| Msa0762390 | AT1G27940.1 | 37.549 | 506 | 286 | 11 | 268 | 764 | 124 | 608 | 2.64e-82 | 286 |
| Msa0762390 | AT1G27940.1 | 32.014 | 556 | 317 | 13 | 232 | 764 | 725 | 1242 | 1.12e-68 | 246 |
| Msa0762390 | AT1G28010.1 | 38.340 | 506 | 282 | 12 | 268 | 764 | 125 | 609 | 2.29e-80 | 281 |
| Msa0762390 | AT1G28010.1 | 33.792 | 509 | 303 | 9 | 268 | 764 | 758 | 1244 | 2.88e-67 | 243 |
| Msa0762390 | AT4G01830.1 | 35.968 | 506 | 295 | 10 | 268 | 764 | 106 | 591 | 6.46e-75 | 265 |
| Msa0762390 | AT4G01830.1 | 33.922 | 510 | 303 | 12 | 266 | 762 | 735 | 1223 | 1.13e-66 | 241 |
| Msa0762390 | AT2G47000.6 | 34.320 | 507 | 304 | 9 | 266 | 762 | 792 | 1279 | 8.72e-75 | 265 |
Find 199 sgRNAs with CRISPR-Local
Find 695 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCATACCAAACGGTAAC+GGG | 0.454848 | 5_2:+20538581 | None:intergenic |
| AAAGCGCTGCATATGGATTT+TGG | 0.200298 | 5_2:-20535457 | Msa0762390:CDS |
| AAATCAGGAGGATATTGAGT+GGG | 0.636760 | 5_2:-20532739 | Msa0762390:CDS |
| AACGAGGAAGAAGATGATGA+TGG | 0.504680 | 5_2:+20538969 | None:intergenic |
| AACTCTACCTGACCACTTGT+AGG | 0.450787 | 5_2:+20534317 | None:intergenic |
| AAGAAACCTTTGAAAAGTGT+AGG | 0.564388 | 5_2:+20540755 | None:intergenic |
| AAGAAACTCGTTAGAAGCAT+TGG | 0.448929 | 5_2:+20538703 | None:intergenic |
| AAGAAGAGAGGGTGAAATTG+TGG | 0.535051 | 5_2:+20538810 | None:intergenic |
| AAGACTTGGATGTCATGTGG+TGG | 0.696777 | 5_2:-20532934 | Msa0762390:CDS |
| AAGAGAGGGTGAAATTGTGG+CGG | 0.653474 | 5_2:+20538813 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTGTATTTGTATAAGGGATT+TGG | - | chr5_2:20531078-20531097 | Msa0762390:three_prime_UTR | 30.0% | |
| GTATTTGTATAAGGGATTTG+GGG | - | chr5_2:20531080-20531099 | Msa0762390:three_prime_UTR | 30.0% | |
| TGAATATTGGGACAAATAAG+AGG | - | chr5_2:20531391-20531410 | Msa0762390:three_prime_UTR | 30.0% | |
| ATAAACAGCTAAAACCAAGA+AGG | + | chr5_2:20531485-20531504 | None:intergenic | 30.0% | |
| ACACTAGTAAGATAACTAAG+AGG | - | chr5_2:20531598-20531617 | Msa0762390:CDS | 30.0% | |
| CACTAGTAAGATAACTAAGA+GGG | - | chr5_2:20531599-20531618 | Msa0762390:CDS | 30.0% | |
| ACCATAACAACACATAATTC+TGG | + | chr5_2:20531961-20531980 | None:intergenic | 30.0% | |
| CAAAAAAAATATATCCTGGC+GGG | + | chr5_2:20532027-20532046 | None:intergenic | 30.0% | |
| CAAAAAAAATATATCCTGGC+GGG | + | chr5_2:20532027-20532046 | None:intergenic | 30.0% | |
| TGTATTTATAGAGAAGTTGG+GGG | - | chr5_2:20532250-20532269 | Msa0762390:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 20531060 | 20540991 | 20531060 | ID=Msa0762390;Name=Msa0762390 |
| chr5_2 | mRNA | 20531060 | 20540991 | 20531060 | ID=Msa0762390-mRNA-1;Parent=Msa0762390;Name=Msa0762390-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0.73|0.75|0.85|1|1|20|524|769 |
| chr5_2 | exon | 20531060 | 20531664 | 20531060 | ID=Msa0762390-mRNA-1:exon:8682;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20532053 | 20532113 | 20532053 | ID=Msa0762390-mRNA-1:exon:8681;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20532189 | 20532253 | 20532189 | ID=Msa0762390-mRNA-1:exon:8680;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20532542 | 20532823 | 20532542 | ID=Msa0762390-mRNA-1:exon:8679;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20532907 | 20532981 | 20532907 | ID=Msa0762390-mRNA-1:exon:8678;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20533235 | 20533267 | 20533235 | ID=Msa0762390-mRNA-1:exon:8677;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20534325 | 20534399 | 20534325 | ID=Msa0762390-mRNA-1:exon:8676;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20534489 | 20534545 | 20534489 | ID=Msa0762390-mRNA-1:exon:8675;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20534671 | 20534744 | 20534671 | ID=Msa0762390-mRNA-1:exon:8674;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20535030 | 20535240 | 20535030 | ID=Msa0762390-mRNA-1:exon:8673;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20535421 | 20535523 | 20535421 | ID=Msa0762390-mRNA-1:exon:8672;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20535620 | 20535690 | 20535620 | ID=Msa0762390-mRNA-1:exon:8671;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20535781 | 20535835 | 20535781 | ID=Msa0762390-mRNA-1:exon:8670;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20536064 | 20536167 | 20536064 | ID=Msa0762390-mRNA-1:exon:8669;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20536252 | 20536374 | 20536252 | ID=Msa0762390-mRNA-1:exon:8668;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20536476 | 20536520 | 20536476 | ID=Msa0762390-mRNA-1:exon:8667;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20538327 | 20538410 | 20538327 | ID=Msa0762390-mRNA-1:exon:8666;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20538505 | 20539076 | 20538505 | ID=Msa0762390-mRNA-1:exon:8665;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20540762 | 20540810 | 20540762 | ID=Msa0762390-mRNA-1:exon:8664;Parent=Msa0762390-mRNA-1 |
| chr5_2 | exon | 20540902 | 20540991 | 20540902 | ID=Msa0762390-mRNA-1:exon:8663;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20540902 | 20540991 | 20540902 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20540762 | 20540810 | 20540762 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20538505 | 20539076 | 20538505 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20538327 | 20538410 | 20538327 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20536476 | 20536520 | 20536476 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20536252 | 20536374 | 20536252 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20536064 | 20536167 | 20536064 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20535781 | 20535835 | 20535781 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20535620 | 20535690 | 20535620 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20535421 | 20535523 | 20535421 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20535030 | 20535240 | 20535030 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20534671 | 20534744 | 20534671 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20534489 | 20534545 | 20534489 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20534325 | 20534399 | 20534325 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20533235 | 20533267 | 20533235 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20532907 | 20532981 | 20532907 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20532542 | 20532823 | 20532542 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20532189 | 20532253 | 20532189 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20532053 | 20532113 | 20532053 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | CDS | 20531584 | 20531664 | 20531584 | ID=Msa0762390-mRNA-1:cds;Parent=Msa0762390-mRNA-1 |
| chr5_2 | three_prime_UTR | 20531060 | 20531583 | 20531060 | ID=Msa0762390-mRNA-1:three_prime_utr;Parent=Msa0762390-mRNA-1 |
| Gene Sequence |
>Msa0762390 ATGAAGATTAGGGTTTTGGTGTATTTGTATAAGGGATTTGGGGAGGTTTACGTTTTTGTCCTTAGGGTTTGTGCGTTTTCAGACATGGAGGGGATGAGTCAATTGATAATTACTCTTCGGACCCCTACACTTTTCAAAGGAGAAAGAACAACTTCCATTGCCGCTTCTAATACACATGAACCACATAACACTCTTCTCAAACTTCTACCTTTCATCTCAAAAACCCATCATCATCTTCTTCCTCGTTACTTCGCTACCACCACCATGCCTCCACCACTCACCGGCACACATCACTATCATTACTTACTTCCTCCTTCAACCTCTCACCACCGCCATCAACGCCACCGCCACCAACCTCTAATCACATTCTCCTCCGTCAACCGCCACAATTTCACCCTCTCTTCTTCCAAACGCATTCTCTTATCCTCTTCAATAAAATCAGCTTCCATCAATGAAGTTTCAATCCACAACAGTTCAGAATCAGCAGCTTCCAATGCTTCTAACGAGTTTCTTGATAGAATACGCAAATGGGTTTCTTTCCTTCCTTCTATTTTCCCTGGAGGAACATGGTGGAACTTCTCCGATGACGTCGAGGTTTCCATGTTCAATCAACCCGTTACCGTTTGGTATGCTTTGGGTAAAATGTGGAACCTTGTGGCGAAAGATAGATGGGTTATTTTCGCTGCTTTTTCTGCTCTTATCATTGCAGCAGTTTCGGAGATTTCTATACCACATTTTTTGACAGCATCGATATTCTCGGCGCAAAGTGGTGATATTAAAGTTTTTCATGGGAATGTTAAGAGAATGAGAGAAACATTATACTCTTCCCTTCTTCTTCAGGATATATCATTTTTTGACAATGAAACAGTAGGTGATTTGACAAGTAGACTTGGGGCAGATTGTCAGCAAGTTTCAAGGGTTATTGGAAATGATCTTAATTTGATACTGCGCAATGTCCTTCAGGGAGGAGGTTCATTGATCTACTTGTTGATTTTGTCTTGGCCACTTGGTTTATGTACGCTGGTGATATGCTCCATATTAGCAGCGGTCATGTTACGTTATGGATGGTACCAGAAGAAGGCAGCAAGGTTGATCCAAGAAGTCACTGCTTCTGCGAATAATGTAGCTCAAGAGACATTCTCTCTTATTAGAACTGTTCGAGTTTATGGAACCGAAGAAGAAGAACACGGAAGGTACAAATGGTGGCTTGAAAAATTAGCTGACATAAGCTTGCGACAAAGCGCTGCATATGGATTTTGGAATTTCAGCTTCAACACTCTTTATCATTCAACTCAGATTATTGCTGTGCTGTTTGGAGGAATGTCTATTCTATCGGGTCATATTACAGCAGAGAAACTTACTAAGTTTATACTATATAGTGAATGGTTAATTTATTCAACCTGGTGGGTGGGGGATAATGTATCCAATTTAATGCAATCAGTTGGAGCTAGTGAAAAGGTGTTCAACTTGATGGATCTCTCACCTAGCAGCCAATTTATAACAGAAGGAATGAAGTTGCAGAGTTTAACGGGGCATATTGAGTTTGTAAATGTATCTTTTCACTATCCTTCAAGACCAACGGTACACGTAGTGCAACGTGTGAACTTTGTTGTCAATCCTAGTGAGGTGGTTGCCATTGTTGGTCTTAGTGGTAGCGGGAAAAGCACATTGGTGAATCTATTACTCCGTCTCTACGAGCCTACAAGTGGTCAGGTGCTTATACCTTCTATTCTAGATTCTTTACTGATTTTGATAGACGGTGTCCCTCACAAAGACTTGGATGTCATGTGGTGGAGAGAGAGGATCGGATATGTGGGACAGGAACCGAAGTTATTCCGGATGGATATTAGCTCGAACATCAGATATGGATGTACCCGTGATGTAAATCAGGAGGATATTGAGTGGGCTGCAAAACAGGCCTACGCTCATGACTTTATCTCAGCACTTCCTAGTGGTTATGAAACACTTGTTGATGACGATCTTTTAAGTGGCGGACAAAAGCAACGAATTGCTATCGCTAGGGCCATTCTTAGGGACCCGAAAATATTGATTCTTGATGAAGCCACTAGTGCACTGGATGCTGAAAGTGAACATAATGTGAAGGGTGTTCTTCGATCGGTTAGAAGTGATTCTTCATCAAGGAGAAGTGTCATAGTCATTGCACACAGGCTTTCTACCATACAAGCTGCTGACAGGATTATTGTGATGGATAAGGGTCAAATAGTTGAGAATGGAAGTCACAGGGAACTTCTCTTGAAAGATGGTTTATATGCTCGATTGACTAGAAAACAGGCTGATACCATGGAATGA |
| Protein sequence |
>Msa0762390 MKIRVLVYLYKGFGEVYVFVLRVCAFSDMEGMSQLIITLRTPTLFKGERTTSIAASNTHEPHNTLLKLLPFISKTHHHLLPRYFATTTMPPPLTGTHHYHYLLPPSTSHHRHQRHRHQPLITFSSVNRHNFTLSSSKRILLSSSIKSASINEVSIHNSSESAASNASNEFLDRIRKWVSFLPSIFPGGTWWNFSDDVEVSMFNQPVTVWYALGKMWNLVAKDRWVIFAAFSALIIAAVSEISIPHFLTASIFSAQSGDIKVFHGNVKRMRETLYSSLLLQDISFFDNETVGDLTSRLGADCQQVSRVIGNDLNLILRNVLQGGGSLIYLLILSWPLGLCTLVICSILAAVMLRYGWYQKKAARLIQEVTASANNVAQETFSLIRTVRVYGTEEEEHGRYKWWLEKLADISLRQSAAYGFWNFSFNTLYHSTQIIAVLFGGMSILSGHITAEKLTKFILYSEWLIYSTWWVGDNVSNLMQSVGASEKVFNLMDLSPSSQFITEGMKLQSLTGHIEFVNVSFHYPSRPTVHVVQRVNFVVNPSEVVAIVGLSGSGKSTLVNLLLRLYEPTSGQVLIPSILDSLLILIDGVPHKDLDVMWWRERIGYVGQEPKLFRMDISSNIRYGCTRDVNQEDIEWAAKQAYAHDFISALPSGYETLVDDDLLSGGQKQRIAIARAILRDPKILILDEATSALDAESEHNVKGVLRSVRSDSSSRRSVIVIAHRLSTIQAADRIIVMDKGQIVENGSHRELLLKDGLYARLTRKQADTME* |
