AlfalfaGEDB | Msa1177360

M. sativa cultivar zhongmu-4 / Msa1177360

KEGG: K08237
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0006805 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008194 GO:0009056 GO:0009058 GO:0009410 GO:0009628 GO:0009636 GO:0009651 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009987 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0019438 GO:0019748 GO:0035251 GO:0042178 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046527 GO:0050896 GO:0051716 GO:0070887 GO:0071466 GO:0071704 GO:0080043 GO:0080044 GO:1901360 GO:1901362 GO:1901576
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177360	sp\|Q9AR73\|HQGT_RAUSE	56.897	464	195	3	14	473	2	464	0.0	560

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177360	A0A072TN95	95.825	479	20	0	1	479	1	479	0.0	951

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1177360	0.867495	1.312039e-65	-8.615850e-47
Msa0002700	Msa1177360	0.806066	9.849980e-50	-8.615850e-47
Msa0005500	Msa1177360	0.866072	3.724154e-65	-8.615850e-47
Msa0014970	Msa1177360	0.809739	1.630473e-50	-8.615850e-47
Msa0021570	Msa1177360	0.802354	5.837142e-49	-8.615850e-47
Msa0022230	Msa1177360	0.828569	8.359658e-55	-8.615850e-47
Msa0022960	Msa1177360	0.800171	1.633385e-48	-8.615850e-47
Msa0024490	Msa1177360	0.836680	8.119053e-57	-8.615850e-47
Msa0025060	Msa1177360	0.838516	2.744883e-57	-8.615850e-47
Msa0025090	Msa1177360	0.858899	5.998551e-63	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177360	MtrunA17_Chr8g0347281	95.825	479	20	0	1	479	1	479	0.0	951
Msa1177360	MtrunA17_Chr8g0347271	92.243	477	37	0	3	479	4	480	0.0	922
Msa1177360	MtrunA17_Chr7g0275511	59.833	478	176	5	4	472	5	475	0.0	576
Msa1177360	MtrunA17_Chr6g0449931	49.247	465	227	5	19	475	7	470	1.47e-149	435
Msa1177360	MtrunA17_Chr8g0336651	47.974	469	230	5	19	476	782	1247	1.64e-149	458
Msa1177360	MtrunA17_Chr8g0336651	46.638	461	236	5	19	472	7	464	3.15e-138	428
Msa1177360	MtrunA17_Chr8g0336651	46.316	285	111	4	198	475	528	777	3.34e-72	248
Msa1177360	MtrunA17_Chr7g0232411	45.356	463	242	5	19	473	7	466	3.31e-149	434
Msa1177360	MtrunA17_Chr7g0232391	45.572	463	241	5	19	473	7	466	3.55e-148	431
Msa1177360	MtrunA17_Chr1g0153451	46.908	469	236	7	19	479	7	470	4.55e-148	431

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177360	AT4G01070.1	57.328	464	191	5	17	474	7	469	0.0	539
Msa1177360	AT1G01420.1	52.688	465	210	7	17	474	7	468	4.63e-171	490
Msa1177360	AT1G01420.2	52.688	465	210	7	17	474	10	471	4.70e-171	490
Msa1177360	AT1G01390.1	51.620	463	217	5	17	473	7	468	2.82e-159	460
Msa1177360	AT1G01390.2	51.620	463	217	5	17	473	24	485	3.68e-159	461
Msa1177360	AT4G01070.2	53.644	343	150	6	17	353	7	346	9.78e-118	349
Msa1177360	AT5G26310.1	38.429	471	266	11	13	468	2	463	2.56e-104	320
Msa1177360	AT5G66690.1	38.004	471	268	11	13	468	2	463	8.57e-101	311
Msa1177360	AT2G18570.1	36.998	473	269	12	17	471	4	465	7.29e-99	305
Msa1177360	AT3G50740.1	37.500	464	261	11	17	460	6	460	1.63e-98	305

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Find 149 sgRNAs with CRISPR-Local

Find 358 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGATAGAACGAAGGA+TGG	0.568943	8_1:+9725589	None:intergenic
AAAAGTTCAGGGCGAGTTTA+GGG	0.316326	8_1:-9725390	Msa1177360:CDS
AAACAGATGAAATTGCTTAT+GGG	0.403797	8_1:-9725016	Msa1177360:CDS
AAACATCCAGGGATGTTAAG+CGG	0.681929	8_1:+9725356	None:intergenic
AAAGGACAAATTATTGGCAG+TGG	0.572658	8_1:+9725439	None:intergenic
AAAGGCAAAGGACAAATTAT+TGG	0.313826	8_1:+9725433	None:intergenic
AAATAGGTCGATAATGAGAG+CGG	0.695005	8_1:+9725523	None:intergenic
AACAAAGCAAATATGTGAAT+TGG	0.358779	8_1:-9724499	Msa1177360:CDS
AACGGTCCAACCGGATAAAC+CGG	0.383526	8_1:+9725164	None:intergenic
AAGACGTGGGAAATGCCATG+AGG	0.744338	8_1:+9725677	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGCAAAGGACAAATTAT+TGG	+	chr8_1:9724915-9724934	None:intergenic	30.0%
ATAGAGTCTTCATTCACTAA+CGG	+	chr8_1:9725202-9725221	None:intergenic	30.0%
CAAACAGATGAAATTGCTTA+TGG	-	chr8_1:9725328-9725347	Msa1177360:CDS	30.0%
ATTGTGAAGAGATTAATGGT+AGG	-	chr8_1:9725742-9725761	Msa1177360:CDS	30.0%
GAAGATTCGTTATAGAATGA+AGG	-	chr8_1:9725777-9725796	Msa1177360:CDS	30.0%
AAAGGCAAAGGACAAATTAT+TGG	+	chr8_1:9724915-9724934	None:intergenic	30.0%
ATAGAGTCTTCATTCACTAA+CGG	+	chr8_1:9725202-9725221	None:intergenic	30.0%
CAAACAGATGAAATTGCTTA+TGG	-	chr8_1:9725328-9725347	Msa1177360:CDS	30.0%
ATTGTGAAGAGATTAATGGT+AGG	-	chr8_1:9725742-9725761	Msa1177360:CDS	30.0%
GAAGATTCGTTATAGAATGA+AGG	-	chr8_1:9725777-9725796	Msa1177360:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	9724464	9725903	9724464	ID=Msa1177360;Name=Msa1177360
chr8_1	mRNA	9724464	9725903	9724464	ID=Msa1177360-mRNA-1;Parent=Msa1177360;Name=Msa1177360-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|479
chr8_1	exon	9724464	9725903	9724464	ID=Msa1177360-mRNA-1:exon:3364;Parent=Msa1177360-mRNA-1
chr8_1	CDS	9724464	9725903	9724464	ID=Msa1177360-mRNA-1:cds;Parent=Msa1177360-mRNA-1

Gene Sequence

>Msa1177360

ATGGAATATCAACAACAATCCCTCACCACACCAAAACATGAACTACCACCTCTAGTAGCCATGTTTCCAACTCCAGGAATGGGACATCTCATTCCAATGATCGAATTCGCCAAGAGACTCTCCAAACATAACCTCCCCATCACCTTCATCATCCCCAACGACGGTCCACCTTCAAAGGCTCAAACCACCGTACTCACCTCCCTACCTCATGGCATTTCCCACGTCTTCCTCCCTCCGGTCACCCTCTCCGACCTCCCACCCAACACCAAACCTGAACCACTCATGATAGCCACCATCCTTCGTTCTATCCCTTTTCTCCATCAAACCCTATTCTCTCTCAAGACTTCCCACCGTCTCTCCGCTCTCATTATCGACCTATTTGGCACCGATGCCTTCGACATGGCCGCTGAACTAGATATCCCTTCATATCTCTACTTCCCTTCCACTGCCAATAATTTGTCCTTTGCCTTTTATTTTCCCCAACTCGACCAAAAAGTTCAGGGCGAGTTTAGGGATATCCCTGAACCGCTTAACATCCCTGGATGTTTCGCGGTTCACGGGAAAGACTTACCAGACCCGGTTCAGGATCGAAACGACGAAGCCTACAAGTGTTTTCTCCACCAAATGACAAGATACAGGTTGGCTAAAGGAATAATAGAGAATAGTTTTTTTGAGCTTGAACCGGAGGCTATTACTTTTTTGCAAAAGAATGAACCACCGGTTTATCCGGTTGGACCGTTAGTGAATGAAGACTCTATTAATAATGGATCTGAATTTGATGTGTGTTTTCAATGGTTGGATGAGCAGCCACGTGGAAGTGTTATATTTGTGAATTTTGGTAGTGAGGGGATCCTTACTAGTGCTCAAACAGATGAAATTGCTTATGGGTTGGAAATGAGTGAACAGAGGTTCTTGTGGGTTTTAAGGTGTCCTAAGGATAAAGTTGAAAATGATTCTAATTTTATTGCTAATAGTAATGTTGACCCTTTTGAATTTTTACCAAATGGTTTTATAGAGAGGACTAAAGGGACGGGTTTAGTGCTCCCATATTGGGCCCCACAAGCACAAGTTTTGAGCCATATCTCAATTGGAGGGTTTGTGAGTCATTGTGGTTGGAATTCAACTCTTGAGAGTGTGGTGAATGGTGTGCCTTTGATTGCATGGCCACTCTATGCTGAACAAAAGATGAATGCTGTTCTTTTGAGTGAAAAAGTTAAAGTGGCTATTAGACCAGAAGTTGGTGAAAATGGGTTGGTTCAAAGGGAAGAAATTGCAAGTATTGTGAAGAGATTAATGGTAGGAGAGGAAGAGCAGAAGATTCGTTATAGAATGAAGGATCTCAAAGATGCAGCTACTAATGCTCTAAAGGAAAATGGTTCTTCAACAAAGCAAATATGTGAATTGGCTCTCAAATGGAAAGGTGTGACAATACCAAATTGA

Protein sequence

>Msa1177360

MEYQQQSLTTPKHELPPLVAMFPTPGMGHLIPMIEFAKRLSKHNLPITFIIPNDGPPSKAQTTVLTSLPHGISHVFLPPVTLSDLPPNTKPEPLMIATILRSIPFLHQTLFSLKTSHRLSALIIDLFGTDAFDMAAELDIPSYLYFPSTANNLSFAFYFPQLDQKVQGEFRDIPEPLNIPGCFAVHGKDLPDPVQDRNDEAYKCFLHQMTRYRLAKGIIENSFFELEPEAITFLQKNEPPVYPVGPLVNEDSINNGSEFDVCFQWLDEQPRGSVIFVNFGSEGILTSAQTDEIAYGLEMSEQRFLWVLRCPKDKVENDSNFIANSNVDPFEFLPNGFIERTKGTGLVLPYWAPQAQVLSHISIGGFVSHCGWNSTLESVVNGVPLIAWPLYAEQKMNAVLLSEKVKVAIRPEVGENGLVQREEIASIVKRLMVGEEEQKIRYRMKDLKDAATNALKENGSSTKQICELALKWKGVTIPN*