AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0591570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0591570 MtrunA17_Chr4g0009641 88.462 390 20 2 1 390 1 365 0.0 697
Msa0591570 MtrunA17_Chr4g0009681 82.429 387 67 1 4 390 1 386 0.0 667
Msa0591570 MtrunA17_Chr4g0009701 83.077 390 65 1 1 390 1 389 0.0 664
Msa0591570 MtrunA17_Chr4g0009661 82.564 390 67 1 1 390 1 389 0.0 658
Msa0591570 MtrunA17_Chr4g0009651 82.051 390 69 1 1 390 1 389 0.0 651
Msa0591570 MtrunA17_Chr4g0009711 80.000 390 77 1 1 390 1 389 0.0 648
Msa0591570 MtrunA17_Chr4g0009671 80.233 344 68 0 47 390 11 354 0.0 574
Msa0591570 MtrunA17_Chr4g0009721 69.251 387 70 3 4 390 1 338 0.0 538
Msa0591570 MtrunA17_Chr2g0285351 57.632 380 153 5 18 390 10 388 2.96e-156 448
Msa0591570 MtrunA17_Chr3g0133011 56.477 386 153 6 20 390 30 415 4.37e-153 441
Msa0591570 MtrunA17_Chr2g0285381 58.424 368 139 7 29 390 25 384 4.90e-146 422
Msa0591570 MtrunA17_Chr2g0285371 56.522 368 153 5 29 390 22 388 5.33e-145 419
Msa0591570 MtrunA17_Chr3g0093971 38.786 379 214 8 15 390 6 369 3.45e-90 279
Msa0591570 MtrunA17_Chr8g0338461 36.429 420 221 11 10 390 2 414 4.10e-79 251
Msa0591570 MtrunA17_Chr7g0249431 33.333 423 220 12 19 390 6 417 7.70e-74 238
Msa0591570 MtrunA17_Chr7g0234051 34.872 390 213 8 39 390 28 414 3.37e-72 234
Msa0591570 MtrunA17_Chr7g0234041 32.683 410 206 12 30 390 16 404 2.08e-60 202
Msa0591570 MtrunA17_Chr2g0279371 38.579 197 98 4 16 211 9 183 1.68e-39 139
Msa0591570 MtrunA17_Chr2g0309511 32.615 325 194 13 44 361 43 349 5.70e-36 137
Msa0591570 MtrunA17_Chr2g0309521 31.692 325 197 12 44 361 43 349 6.69e-36 137
Msa0591570 MtrunA17_Chr2g0281621 30.149 335 212 14 41 366 36 357 5.05e-35 134
Msa0591570 MtrunA17_Chr4g0038311 28.612 353 234 11 19 364 13 354 6.40e-35 134
Msa0591570 MtrunA17_Chr2g0309541 31.098 328 197 14 44 361 42 350 7.05e-35 134
Msa0591570 MtrunA17_Chr2g0318911 33.333 288 169 12 17 297 23 294 7.76e-35 134
Msa0591570 MtrunA17_Chr3g0094811 27.366 391 243 14 13 390 6 368 4.58e-33 129
Msa0591570 MtrunA17_Chr4g0068861 31.228 285 166 12 17 291 57 321 8.04e-33 128
Msa0591570 MtrunA17_Chr1g0199991 30.387 362 221 15 12 361 1 343 1.35e-32 127
Msa0591570 MtrunA17_Chr2g0304931 28.460 383 219 15 29 390 28 376 1.50e-32 127
Msa0591570 MtrunA17_Chr4g0028281 39.344 183 103 4 70 250 109 285 1.98e-32 127
Msa0591570 MtrunA17_Chr1g0199971 28.652 356 225 14 16 361 7 343 2.42e-32 126
Msa0591570 MtrunA17_Chr1g0153881 30.275 327 195 13 40 361 89 387 7.29e-32 125
Msa0591570 MtrunA17_Chr4g0047861 29.499 339 215 13 41 368 54 379 6.90e-31 123
Msa0591570 MtrunA17_Chr3g0126031 28.354 328 210 12 41 361 29 338 1.89e-30 121
Msa0591570 MtrunA17_Chr1g0171411 36.842 190 112 4 70 257 97 280 1.97e-30 122
Msa0591570 MtrunA17_Chr4g0028261 40.000 175 97 4 57 230 120 287 2.79e-30 121
Msa0591570 MtrunA17_Chr3g0090331 30.451 266 168 10 40 299 74 328 2.82e-30 121
Msa0591570 MtrunA17_Chr4g0068841 27.128 376 227 14 17 379 10 351 3.88e-30 120
Msa0591570 MtrunA17_Chr3g0118831 28.182 330 201 13 40 361 77 378 5.53e-30 120
Msa0591570 MtrunA17_Chr2g0327401 37.368 190 112 5 70 257 114 298 7.15e-30 120
Msa0591570 MtrunA17_Chr4g0028241 40.854 164 90 3 70 232 103 260 1.05e-29 119
Msa0591570 MtrunA17_Chr3g0131731 27.174 368 235 11 37 390 27 375 1.37e-29 119
Msa0591570 MtrunA17_Chr1g0153941 27.193 342 213 14 28 361 64 377 1.80e-29 119
Msa0591570 MtrunA17_Chr4g0028271 39.429 175 98 4 57 230 99 266 1.89e-29 119
Msa0591570 MtrunA17_Chr2g0327381 40.741 162 89 5 70 229 108 264 5.95e-29 118
Msa0591570 MtrunA17_Chr3g0118891 28.485 330 199 13 40 361 77 377 1.17e-28 116
Msa0591570 MtrunA17_Chr7g0219051 29.514 288 184 11 19 299 13 288 1.19e-28 116
Msa0591570 MtrunA17_Chr3g0126051 27.100 369 231 18 19 370 11 358 3.63e-28 115
Msa0591570 MtrunA17_Chr7g0221551 29.297 256 163 7 16 263 6 251 4.62e-28 114
Msa0591570 MtrunA17_Chr5g0431301 27.928 333 209 15 41 361 30 343 5.99e-28 114
Msa0591570 MtrunA17_Chr1g0199171 36.126 191 111 6 70 257 104 286 9.29e-28 114
Msa0591570 MtrunA17_Chr8g0385871 28.142 366 238 14 34 390 27 376 1.60e-27 113
Msa0591570 MtrunA17_Chr4g0020241 31.461 267 164 11 40 300 73 326 5.41e-27 112
Msa0591570 MtrunA17_Chr3g0118851 27.660 329 204 13 40 361 77 378 6.28e-27 112
Msa0591570 MtrunA17_Chr2g0327371 37.634 186 94 6 44 229 79 242 6.60e-27 112
Msa0591570 MtrunA17_Chr3g0118841 27.439 328 205 12 40 361 78 378 1.09e-26 111
Msa0591570 MtrunA17_Chr1g0153931 33.166 199 122 8 28 221 62 254 6.18e-26 105
Msa0591570 MtrunA17_Chr1g0165811 31.780 236 138 9 40 269 30 248 2.20e-25 103
Msa0591570 MtrunA17_Chr3g0118881 27.761 335 199 16 38 361 75 377 2.78e-25 107
Msa0591570 MtrunA17_Chr3g0118861 28.022 364 211 18 40 382 75 408 6.04e-25 105
Msa0591570 MtrunA17_Chr2g0281641 27.551 294 187 11 88 368 1 281 1.17e-23 101
Msa0591570 MtrunA17_Chr5g0447441 23.889 360 223 12 47 391 30 353 2.03e-18 86.7
Msa0591570 MtrunA17_Chr4g0044051 29.555 247 133 12 57 279 47 276 7.56e-17 82.0
Msa0591570 MtrunA17_Chr2g0279381 27.684 177 109 4 225 390 4 172 2.13e-16 77.4
Msa0591570 MtrunA17_Chr1g0204191 25.519 337 181 16 40 361 91 372 2.51e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0591570 AT5G09640.1 49.086 383 179 8 20 390 6 384 3.42e-122 362
Msa0591570 AT2G22970.4 50.139 361 153 4 33 390 17 353 3.79e-119 352
Msa0591570 AT2G22970.3 50.139 361 153 4 33 390 17 353 9.17e-119 352
Msa0591570 AT2G22970.1 50.139 361 153 4 33 390 17 353 1.31e-118 351
Msa0591570 AT2G23000.1 48.895 362 163 6 31 390 16 357 1.47e-118 351
Msa0591570 AT2G23000.2 48.895 362 163 6 31 390 16 357 1.47e-118 351
Msa0591570 AT2G22980.2 49.176 364 152 8 32 391 17 351 2.81e-116 345
Msa0591570 AT2G22920.1 50.000 366 154 7 30 390 14 355 4.43e-116 344
Msa0591570 AT2G22920.2 50.000 366 154 7 30 390 14 355 5.02e-116 345
Msa0591570 AT2G22980.4 49.311 363 151 8 32 390 17 350 5.98e-116 345
Msa0591570 AT2G22980.1 49.311 363 151 8 32 390 17 350 8.58e-116 344
Msa0591570 AT2G23010.2 48.128 374 173 5 19 390 3 357 8.71e-116 344
Msa0591570 AT2G23010.1 48.128 374 173 5 19 390 3 357 1.19e-115 344
Msa0591570 AT2G22980.3 48.907 366 152 9 32 391 17 353 2.99e-115 340
Msa0591570 AT1G33540.1 47.778 360 180 6 38 390 26 384 4.60e-114 341
Msa0591570 AT3G12240.1 48.588 354 155 6 39 389 26 355 6.98e-113 337
Msa0591570 AT1G73270.2 49.307 361 155 7 33 390 26 361 7.73e-113 337
Msa0591570 AT1G73270.1 49.307 361 155 7 33 390 26 361 8.21e-113 337
Msa0591570 AT1G73280.2 45.989 374 175 5 21 391 13 362 1.60e-112 334
Msa0591570 AT2G22990.4 45.833 360 171 6 33 390 16 353 9.12e-112 333
Msa0591570 AT2G22990.3 45.833 360 171 6 33 390 16 353 1.33e-111 334
Msa0591570 AT2G22990.1 45.833 360 171 6 33 390 16 353 1.68e-111 333
Msa0591570 AT3G12203.3 48.315 356 156 6 38 390 6 336 2.76e-111 332
Msa0591570 AT3G12220.1 49.014 355 154 6 39 390 25 355 5.24e-111 332
Msa0591570 AT3G12203.1 46.561 378 174 6 16 390 5 357 5.34e-111 332
Msa0591570 AT1G73300.1 47.527 364 157 8 33 390 26 361 7.35e-111 332
Msa0591570 AT1G73300.2 47.527 364 157 8 33 390 26 361 7.35e-111 332
Msa0591570 AT3G10450.4 47.753 356 159 6 38 390 26 357 8.44e-111 331
Msa0591570 AT1G73280.1 45.845 373 175 5 21 390 13 361 1.93e-110 331
Msa0591570 AT3G10450.1 47.753 356 159 6 38 390 26 357 3.23e-110 330
Msa0591570 AT3G12230.1 47.458 354 159 6 39 389 25 354 4.25e-110 330
Msa0591570 AT5G36180.1 47.253 364 158 8 33 390 26 361 9.04e-109 327
Msa0591570 AT1G73290.3 48.066 362 156 8 33 390 25 358 5.10e-108 323
Msa0591570 AT1G73310.1 47.368 361 162 6 33 390 26 361 9.00e-108 324
Msa0591570 AT1G73290.1 48.066 362 156 8 33 390 25 358 1.35e-107 323
Msa0591570 AT1G73310.2 47.368 361 162 6 33 390 26 361 1.45e-107 323
Msa0591570 AT2G22970.2 50.000 322 136 3 32 352 16 313 1.53e-106 317
Msa0591570 AT2G22990.5 44.722 360 166 7 33 390 16 344 3.74e-105 317
Msa0591570 AT1G33540.2 46.903 339 172 6 59 390 4 341 1.81e-104 315
Msa0591570 AT2G22920.4 49.846 325 137 5 30 352 14 314 5.24e-104 310
Msa0591570 AT3G12220.2 47.463 335 149 6 59 390 15 325 7.34e-99 300
Msa0591570 AT3G10450.3 45.970 335 154 6 59 390 4 314 2.08e-98 298
Msa0591570 AT3G10450.2 45.970 335 154 6 59 390 4 314 5.60e-98 297
Msa0591570 AT3G12203.2 46.130 323 147 5 71 390 1 299 1.68e-93 285
Msa0591570 AT1G73290.2 52.434 267 121 5 33 297 25 287 1.66e-92 280
Msa0591570 AT3G12220.3 52.893 242 107 4 39 278 25 261 4.88e-82 256
Msa0591570 AT4G12910.2 33.575 414 226 10 18 390 11 416 1.21e-72 234
Msa0591570 AT4G12910.1 33.575 414 226 10 18 390 11 416 2.75e-72 234
Msa0591570 AT5G36180.2 44.286 280 124 6 117 390 6 259 3.70e-70 223
Msa0591570 AT1G73280.4 43.682 277 130 4 117 390 6 259 6.70e-70 223
Msa0591570 AT5G36180.5 44.286 280 124 6 117 390 6 259 7.79e-70 223
Msa0591570 AT5G36180.3 44.286 280 124 6 117 390 6 259 7.79e-70 223
Msa0591570 AT5G36180.4 44.286 280 124 6 117 390 6 259 1.42e-69 223
Msa0591570 AT2G22980.5 44.828 261 112 7 134 390 1 233 4.56e-66 212
Msa0591570 AT2G22990.2 42.085 259 128 4 134 390 1 239 6.55e-66 213
Msa0591570 AT1G73310.3 42.960 277 132 4 117 390 6 259 7.68e-66 213
Msa0591570 AT2G22990.6 42.085 259 128 4 134 390 1 239 9.23e-66 212
Msa0591570 AT3G25420.1 34.021 388 214 9 41 390 30 413 4.50e-65 215
Msa0591570 AT1G73280.3 42.802 257 121 4 137 390 1 234 8.84e-62 201
Msa0591570 AT2G22920.3 45.178 197 81 5 198 390 3 176 1.27e-45 157
Msa0591570 AT5G08260.1 32.441 299 183 11 10 297 4 294 2.08e-38 144
Msa0591570 AT4G30810.1 29.670 364 216 13 15 361 9 349 5.31e-37 140
Msa0591570 AT4G15100.1 30.145 345 210 12 41 368 45 375 6.70e-37 140
Msa0591570 AT5G23210.3 31.690 284 172 10 24 302 37 303 2.52e-35 135
Msa0591570 AT5G23210.1 31.690 284 172 10 24 302 37 303 5.21e-35 135
Msa0591570 AT3G52020.1 31.111 315 193 11 41 352 80 373 2.83e-33 130
Msa0591570 AT1G61130.1 29.296 355 208 13 25 361 16 345 7.24e-33 128
Msa0591570 AT2G35770.1 28.691 359 224 12 12 361 7 342 1.88e-32 127
Msa0591570 AT4G30610.1 34.222 225 136 9 40 259 32 249 3.67e-31 124
Msa0591570 AT2G12480.3 27.350 351 228 13 19 361 8 339 5.74e-31 123
Msa0591570 AT3G17180.2 28.346 381 223 17 33 389 30 384 9.22e-31 122
Msa0591570 AT3G07990.1 28.779 344 211 14 40 374 33 351 1.20e-30 122
Msa0591570 AT3G17180.1 28.346 381 223 17 33 389 30 384 1.87e-30 122
Msa0591570 AT1G11080.2 29.429 350 208 13 41 368 51 383 2.01e-30 122
Msa0591570 AT1G43780.1 30.539 334 204 15 41 363 38 354 2.32e-30 122
Msa0591570 AT1G11080.1 29.429 350 208 13 41 368 51 383 2.44e-30 122
Msa0591570 AT5G22980.1 36.715 207 116 7 70 273 112 306 6.55e-30 120
Msa0591570 AT3G52010.1 26.836 354 226 11 24 368 46 375 6.63e-30 120
Msa0591570 AT2G24000.1 27.778 342 216 12 40 368 30 353 8.51e-30 120
Msa0591570 AT3G02110.1 34.498 229 138 9 35 259 32 252 1.00e-29 120
Msa0591570 AT2G12480.4 28.671 286 186 10 19 297 8 282 1.74e-29 119
Msa0591570 AT2G12480.2 28.671 286 186 10 19 297 8 282 1.91e-29 119
Msa0591570 AT2G12480.1 28.671 286 186 10 19 297 8 282 1.97e-29 119
Msa0591570 AT3G45010.1 29.930 284 173 7 70 350 115 375 5.15e-29 118
Msa0591570 AT2G35780.1 29.964 277 176 10 40 310 29 293 6.62e-29 117
Msa0591570 AT3G52000.1 28.402 338 187 16 41 361 64 363 1.12e-28 117
Msa0591570 AT2G33530.1 29.014 355 211 16 32 371 25 353 4.49e-28 115
Msa0591570 AT2G24010.1 33.482 224 137 9 41 259 31 247 6.22e-27 112
Msa0591570 AT3G10410.1 35.602 191 112 6 70 257 113 295 1.16e-26 111
Msa0591570 AT1G28110.1 26.685 371 229 15 37 390 28 372 1.84e-26 110
Msa0591570 AT1G28110.2 26.685 371 229 15 37 390 28 372 1.84e-26 110
Msa0591570 AT5G42230.1 28.443 334 206 14 41 361 29 342 6.02e-26 109
Msa0591570 AT5G42230.2 28.443 334 206 14 41 361 29 342 6.02e-26 109
Msa0591570 AT5G42240.1 31.696 224 139 8 41 259 33 247 1.72e-25 108
Msa0591570 AT3G56540.1 36.306 157 91 4 41 195 80 229 2.11e-25 104
Msa0591570 AT3G63470.1 27.879 330 206 13 41 363 82 386 4.71e-25 107
Msa0591570 AT2G05850.1 26.970 330 207 14 41 361 64 368 6.89e-24 103
Msa0591570 AT2G24010.4 26.121 379 229 16 41 391 2 357 3.83e-23 99.8
Msa0591570 AT5G22960.1 34.545 165 81 4 57 220 36 174 1.77e-22 94.4
Msa0591570 AT2G24010.2 30.962 239 138 10 41 259 31 262 2.96e-22 98.6
Msa0591570 AT5G23210.5 29.907 214 132 7 94 302 8 208 8.33e-21 93.6
Msa0591570 AT5G23210.4 29.577 213 132 7 95 302 8 207 2.57e-20 91.7
Msa0591570 AT5G23210.2 29.577 213 132 7 95 302 8 207 4.64e-20 91.7
Msa0591570 AT1G15000.1 33.673 196 100 7 42 229 31 204 1.17e-18 87.8
Msa0591570 AT2G24010.3 29.323 266 132 13 41 259 2 258 2.41e-18 87.0
Msa0591570 AT2G22960.1 55.172 58 25 1 334 390 47 104 1.27e-13 69.3

Find 111 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGATCTGTTCCACATTT+TGG 0.131981 4_2:-16070786 Msa0591570:CDS
TCTTAAACACAACTCATTTC+TGG 0.162375 4_2:+16071138 None:intergenic
TCTTATCATCAGTGCCTTTC+AGG 0.165945 4_2:-16070809 Msa0591570:intron
CGAAGCAATGTGAGATTTCT+TGG 0.199174 4_2:-16069925 Msa0591570:three_prime_UTR
ATTCCCATTCCATGAGTAAA+TGG 0.201662 4_2:+16071338 None:intergenic
CCTCTTATGCTTTGGTTAAC+TGG 0.223979 4_2:-16073671 Msa0591570:CDS
TTTCTCTGTGTGTTGTTATA+GGG 0.242077 4_2:+16071375 None:intergenic
TTAACTCATATTGAAGCTTA+TGG 0.264666 4_2:-16074262 Msa0591570:CDS
TCTGGACTTGCCTATCAAAT+AGG 0.272453 4_2:-16073626 Msa0591570:intron
GATACCGCTCTTTGATTTAC+AGG 0.281285 4_2:-16070210 Msa0591570:CDS
TTGAAAATTACCTATTTGAT+AGG 0.283259 4_2:+16073616 None:intergenic
TTTGATAGGTACGTGGGGTT+AGG 0.283274 4_2:-16073761 Msa0591570:intron
TTACAAGAATTATGTGACTA+TGG 0.321671 4_2:+16069826 None:intergenic
TTGATAGGTACGTGGGGTTA+GGG 0.325028 4_2:-16073760 Msa0591570:intron
CGGTTTACTTGCCTCATCAT+AGG 0.341096 4_2:+16071105 None:intergenic
TCCTAAGAAATTGAAGAGTA+TGG 0.358041 4_2:+16072368 None:intergenic
TCAAAGGTTGAACAACTTCC+TGG 0.360669 4_2:-16074238 Msa0591570:CDS
ACCATACTCTTCAATTTCTT+AGG 0.361295 4_2:-16072369 Msa0591570:CDS
AATTACTTGGCAGTTTAGTT+CGG 0.364117 4_2:+16070676 None:intergenic
TGATTAACAGGAAATGAAAA+AGG 0.372135 4_2:-16071582 Msa0591570:intron
TAGGCCCCTTAGCCCCGACT+TGG 0.373159 4_2:+16069884 None:intergenic
TCCTTGATTTCAAATGCAAT+TGG 0.380583 4_2:+16072772 None:intergenic
ATCTTTGACTCAGGAGTTGA+AGG 0.381454 4_2:-16070726 Msa0591570:CDS
ATTACTTGGCAGTTTAGTTC+GGG 0.382908 4_2:+16070677 None:intergenic
TCCAATTGCATTTGAAATCA+AGG 0.383040 4_2:-16072773 Msa0591570:CDS
ATTTGAAATTTCTTGAAGAA+TGG 0.384481 4_2:+16071883 None:intergenic
CCAAGGCGTCTTTCAACTAA+TGG 0.384841 4_2:+16069989 None:intergenic
CGACGGGAGCGTGCCTAGTT+TGG 0.389176 4_2:-16072746 Msa0591570:CDS
GAAATTTCTTGAAGAATGGC+TGG 0.393437 4_2:+16071887 None:intergenic
TAGCCCCAAGTCGGGGCTAA+GGG 0.396573 4_2:-16069889 Msa0591570:three_prime_UTR
TGCAGGGAACCATAGGGAAA+TGG 0.401064 4_2:-16070309 Msa0591570:intron
AGAAAGATCCTCTTATGCTT+TGG 0.405600 4_2:-16073679 Msa0591570:CDS
TTGAAAGACGCCTTGGTTAG+TGG 0.408248 4_2:-16069982 Msa0591570:three_prime_UTR
CTTTGGTTAACTGGTGGTCC+TGG 0.408919 4_2:-16073662 Msa0591570:CDS
GTAGATTTGCCACTTGGAAC+TGG 0.409499 4_2:-16072445 Msa0591570:CDS
AGGCCCCTTAGCCCCGACTT+GGG 0.413614 4_2:+16069885 None:intergenic
AGTCGGGGCTAAGGGGCCTA+AGG 0.413710 4_2:-16069881 Msa0591570:three_prime_UTR
TCACGGATCATCGGAGCGAC+TGG 0.415782 4_2:-16072402 Msa0591570:CDS
TCACATAATTCTTGTAATCA+AGG 0.423696 4_2:-16069820 None:intergenic
CCAGTTAACCAAAGCATAAG+AGG 0.430684 4_2:+16073671 None:intergenic
ATCCCATTTACTCATGGAAT+GGG 0.451576 4_2:-16071341 Msa0591570:CDS
TGGAACAGTAGCCCCAAGTC+GGG 0.454624 4_2:-16069897 Msa0591570:three_prime_UTR
GTAGCCCCAAGTCGGGGCTA+AGG 0.457419 4_2:-16069890 Msa0591570:three_prime_UTR
TGTGAGATTTCTTGGTGTAC+TGG 0.462509 4_2:-16069917 Msa0591570:three_prime_UTR
CAACTAATGGCGAAACCGCT+AGG 0.465235 4_2:+16070002 None:intergenic
GCAGGGAACCATAGGGAAAT+GGG 0.468772 4_2:-16070308 Msa0591570:intron
ATTCTTCAAGAAATTTCAAA+TGG 0.469672 4_2:-16071881 Msa0591570:intron
TATCAAATCCCATTTACTCA+TGG 0.476162 4_2:-16071347 Msa0591570:CDS
GACCCAGTTTCAAGTTCAAA+AGG 0.476471 4_2:+16074199 None:intergenic
TCTTGAAGAATGGCTGGGAC+AGG 0.476940 4_2:+16071893 None:intergenic
CTTTCAAATGAATTCTACAT+TGG 0.479014 4_2:-16071938 Msa0591570:CDS
TTCCCATTCCATGAGTAAAT+GGG 0.481220 4_2:+16071339 None:intergenic
TACAAGAATTATGTGACTAT+GGG 0.484601 4_2:+16069827 None:intergenic
TGTCAGATACTACTAGCTCT+TGG 0.488028 4_2:-16074304 Msa0591570:CDS
TGAGCATTGCAGGGAACCAT+AGG 0.491193 4_2:-16070316 Msa0591570:intron
TGTGGCCTCAAGACCAAACT+AGG 0.493874 4_2:+16072733 None:intergenic
CCTTGAGATTTATCAATGGT+TGG 0.494155 4_2:+16071556 None:intergenic
CCATTAGTTGAAAGACGCCT+TGG 0.496841 4_2:-16069989 Msa0591570:three_prime_UTR
AATCCCATTTACTCATGGAA+TGG 0.496948 4_2:-16071342 Msa0591570:CDS
TACTCTTCAATTTCTTAGGA+AGG 0.501252 4_2:-16072365 Msa0591570:intron
TGAAGTGAATTATGAGAAGG+AGG 0.504769 4_2:+16074337 None:intergenic
CATATATTTGATAGGTACGT+GGG 0.520465 4_2:-16073767 Msa0591570:intron
ACAGCGTTCCCATTTCCCTA+TGG 0.521234 4_2:+16070300 None:intergenic
CACATAATTCTTGTAATCAA+GGG 0.521810 4_2:-16069819 None:intergenic
GAAACCTTGAGATTTATCAA+TGG 0.525518 4_2:+16071552 None:intergenic
GTGGAGAAGATCTTTGACTC+AGG 0.525567 4_2:-16070735 Msa0591570:CDS
GGTAGTGGCCCTTGAAAACC+AGG 0.530817 4_2:+16074220 None:intergenic
GGCCCCTTAGCCCCGACTTG+GGG 0.535208 4_2:+16069886 None:intergenic
AACTAATGGCGAAACCGCTA+GGG 0.537014 4_2:+16070003 None:intergenic
CCAACCATTGATAAATCTCA+AGG 0.544985 4_2:-16071556 Msa0591570:intron
TGGTCTTGAGGCCACAGTCA+TGG 0.546927 4_2:-16072726 Msa0591570:CDS
ATTGGAGCTGATTCATACTC+AGG 0.549342 4_2:-16071920 Msa0591570:CDS
CTTATGCTTTGGTTAACTGG+TGG 0.549628 4_2:-16073668 Msa0591570:CDS
GAGCATTGCAGGGAACCATA+GGG 0.549788 4_2:-16070315 Msa0591570:intron
GCGTGCCTAGTTTGGTCTTG+AGG 0.549911 4_2:-16072738 Msa0591570:CDS
TACTTGCCTCATCATAGGAT+CGG 0.555204 4_2:+16071110 None:intergenic
TATTGAAGCTTATGGCTCAA+AGG 0.556674 4_2:-16074254 Msa0591570:CDS
GTTTCAAGTTCAAAAGGTAG+TGG 0.558447 4_2:+16074205 None:intergenic
TTGAAATCAAGGAGTACGAC+GGG 0.564117 4_2:-16072762 Msa0591570:CDS
AGACGCCTTGGTTAGTGGAG+TGG 0.566889 4_2:-16069977 Msa0591570:three_prime_UTR
TGTTAGCCCATTTAGTGCTG+AGG 0.567452 4_2:+16070554 None:intergenic
GTCGGGGCTAAGGGGCCTAA+GGG 0.569246 4_2:-16069880 Msa0591570:three_prime_UTR
CACAAAAGCGATCCAAAATG+TGG 0.571488 4_2:+16070774 None:intergenic
TAGTGAAGTGAATTATGAGA+AGG 0.571718 4_2:+16074334 None:intergenic
AAATTTCTTGAAGAATGGCT+GGG 0.584151 4_2:+16071888 None:intergenic
TGATAGGTACGTGGGGTTAG+GGG 0.585717 4_2:-16073759 Msa0591570:intron
TAACAATGAGCGTGTTCGCA+AGG 0.589607 4_2:-16070536 Msa0591570:CDS
AGCCCCAAGTCGGGGCTAAG+GGG 0.590066 4_2:-16069888 Msa0591570:three_prime_UTR
CAAGGCACTGCATATTCGAG+AGG 0.592389 4_2:-16070518 Msa0591570:intron
GTGACGATGATCCGCGTCTG+TGG 0.593713 4_2:-16070754 Msa0591570:CDS
CTGGAACAGTAGCCCCAAGT+CGG 0.596352 4_2:-16069898 Msa0591570:three_prime_UTR
GGGTCTTGTGTAAGCCCTAG+CGG 0.606405 4_2:-16070017 Msa0591570:three_prime_UTR
TCATATATTTGATAGGTACG+TGG 0.623494 4_2:-16073768 Msa0591570:intron
AAAATAGCTAGTAAGCCCTT+AGG 0.624722 4_2:+16069865 None:intergenic
ACAAGAATTATGTGACTATG+GGG 0.638767 4_2:+16069828 None:intergenic
GGAACAGATCAAGTCCTGAA+AGG 0.642344 4_2:+16070795 None:intergenic
AGCATAAGAGGATCTTTCTG+AGG 0.643360 4_2:+16073683 None:intergenic
CATGAAACTCAAAGCTACCA+AGG 0.648314 4_2:+16070251 None:intergenic
AACACCTTTGTCCATGACTG+TGG 0.650204 4_2:+16072715 None:intergenic
TTCACCTGTAAATCAAAGAG+CGG 0.660021 4_2:+16070206 None:intergenic
GAGGCCACAGTCATGGACAA+AGG 0.660835 4_2:-16072719 Msa0591570:intron
AAAGATCTTCTCCACAGACG+CGG 0.666238 4_2:+16070743 None:intergenic
AGATCTCCGATCCTATGATG+AGG 0.667078 4_2:-16071116 Msa0591570:intron
ACAAACCACTCCACTAACCA+AGG 0.671218 4_2:+16069972 None:intergenic
GCTTGCAAAGATGAACATGA+TGG 0.680214 4_2:-16074362 Msa0591570:exon
CATTTCTGATGAACTCTACG+CGG 0.688710 4_2:-16071315 Msa0591570:intron
TATGAGAAACCAGTTCCAAG+TGG 0.692436 4_2:+16072436 None:intergenic
ATATATTTGATAGGTACGTG+GGG 0.700917 4_2:-16073766 Msa0591570:intron
TTTGAAATCAAGGAGTACGA+CGG 0.702574 4_2:-16072763 Msa0591570:CDS
GGAACAGTAGCCCCAAGTCG+GGG 0.706355 4_2:-16069896 Msa0591570:three_prime_UTR
CAAGAATTATGTGACTATGG+GGG 0.711146 4_2:+16069829 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTCTTTTCTATTTT+AGG - chr4_2:16070164-16070183 Msa0591570:three_prime_UTR 10.0%
!!! TATTTTCATAATTTCATTAT+TGG - chr4_2:16071771-16071790 Msa0591570:intron 10.0%
!! TTATAATTATAATTACAATG+AGG - chr4_2:16072503-16072522 Msa0591570:intron 10.0%
!! TAAATTATGAAAAAATTACT+TGG + chr4_2:16073639-16073658 None:intergenic 10.0%
!! AAATCAATGTTGAATTATTT+AGG + chr4_2:16071617-16071636 None:intergenic 15.0%
!! ATATAAATCATAGTTCTTAT+TGG + chr4_2:16071669-16071688 None:intergenic 15.0%
!! AATATTAAACAAAGAGAAAT+AGG + chr4_2:16072234-16072253 None:intergenic 15.0%
!!! CTTTGTTTAATATTTACTTT+AGG - chr4_2:16072240-16072259 Msa0591570:intron 15.0%
!!! TATTTAGATTTTTTGTTCAT+CGG + chr4_2:16072804-16072823 None:intergenic 15.0%
!! ATGAACAAAAAATCTAAATA+GGG - chr4_2:16072804-16072823 Msa0591570:intron 15.0%
!! ATAATAGGTATAGATAAAAT+TGG + chr4_2:16073227-16073246 None:intergenic 15.0%
!! TTTAGTATCATCTTAATAAT+AGG + chr4_2:16073242-16073261 None:intergenic 15.0%
!! TAGAAAAAGGAAAATATTAA+GGG - chr4_2:16073370-16073389 Msa0591570:intron 15.0%
!! GAAATAATAGTTTAACAATA+TGG + chr4_2:16073469-16073488 None:intergenic 15.0%
!!! ATCAAATTTGAAACATTTTA+TGG + chr4_2:16073685-16073704 None:intergenic 15.0%
!! TTGAAAATTACCTATTTGAT+AGG + chr4_2:16070686-16070705 None:intergenic 20.0%
!!! ACAGTTTGTTAATACATAAT+TGG - chr4_2:16070740-16070759 Msa0591570:CDS 20.0%
!!! CTTTTTGAAACTATCTAAAA+AGG + chr4_2:16070889-16070908 None:intergenic 20.0%
!!! ACCATAATGTATATCTATTT+TGG - chr4_2:16071114-16071133 Msa0591570:intron 20.0%
!! TCCAAAATAGATATACATTA+TGG + chr4_2:16071118-16071137 None:intergenic 20.0%
!! ATCATCAATTGAATGTTAAT+AGG - chr4_2:16071503-16071522 Msa0591570:intron 20.0%
!! TTCATAATTTCATTATTGGA+AGG - chr4_2:16071775-16071794 Msa0591570:intron 20.0%
!!! TAGTAATGTTAATGATCTTT+TGG - chr4_2:16071809-16071828 Msa0591570:intron 20.0%
!!! ATTTGAAATTTCTTGAAGAA+TGG + chr4_2:16072419-16072438 None:intergenic 20.0%
!! ATTCTTCAAGAAATTTCAAA+TGG - chr4_2:16072418-16072437 Msa0591570:CDS 20.0%
!! TAATCAATTTATTAGAGTGA+GGG + chr4_2:16072560-16072579 None:intergenic 20.0%
!! ATAATCAATTTATTAGAGTG+AGG + chr4_2:16072561-16072580 None:intergenic 20.0%
!! GATGAACAAAAAATCTAAAT+AGG - chr4_2:16072803-16072822 Msa0591570:intron 20.0%
!!! TTAAGATGATACTAAAGTTT+TGG - chr4_2:16073246-16073265 Msa0591570:intron 20.0%
!! AAGAAAAATGAAGTAGAAAA+AGG - chr4_2:16073357-16073376 Msa0591570:intron 20.0%
!! GTAGAAAAAGGAAAATATTA+AGG - chr4_2:16073369-16073388 Msa0591570:intron 20.0%
!! GAAAATATTAAGGGAAAAAA+AGG - chr4_2:16073379-16073398 Msa0591570:intron 20.0%
!! TTCTTATGTTGAATCATAAT+TGG - chr4_2:16073848-16073867 Msa0591570:intron 20.0%
!!! TGAGTTGAACATAATTTTTG+AGG + chr4_2:16069874-16069893 None:intergenic 25.0%
! TTAACTCATATTGAAGCTTA+TGG - chr4_2:16070037-16070056 Msa0591570:three_prime_UTR 25.0%
! ATTTGTAACAGAAAATGGAA+TGG + chr4_2:16070137-16070156 None:intergenic 25.0%
! AGTGAATTTGTAACAGAAAA+TGG + chr4_2:16070142-16070161 None:intergenic 25.0%
! TGTGTGTTCATATATTTGAT+AGG - chr4_2:16070524-16070543 Msa0591570:CDS 25.0%
!! TAGTTTCAAAAAGTGTACTA+AGG - chr4_2:16070896-16070915 Msa0591570:intron 25.0%
! GATACAAAATCTATACTAAC+TGG + chr4_2:16071041-16071060 None:intergenic 25.0%
!!! GTTCGTATTGATTAGAAAAT+TGG + chr4_2:16071096-16071115 None:intergenic 25.0%
!!! TCCATCACTTTTCTTTTATT+AGG - chr4_2:16071271-16071290 Msa0591570:intron 25.0%
! ACCTAATAAAAGAAAAGTGA+TGG + chr4_2:16071275-16071294 None:intergenic 25.0%
! AAGATCATTAACATTACTAC+CGG + chr4_2:16071808-16071827 None:intergenic 25.0%
! TAAGTTCATTATGCTAACTA+GGG - chr4_2:16071957-16071976 Msa0591570:CDS 25.0%
!! GTTAATTTTTCTCATGATGA+AGG - chr4_2:16072142-16072161 Msa0591570:intron 25.0%
! CAATTATCTGTATTGTATAG+TGG - chr4_2:16072317-16072336 Msa0591570:intron 25.0%
! CTTTCAAATGAATTCTACAT+TGG - chr4_2:16072361-16072380 Msa0591570:intron 25.0%
!!! TCTGAAATATTGAGCATTTT+AGG + chr4_2:16072452-16072471 None:intergenic 25.0%
! AAAATGCTCAATATTTCAGA+TGG - chr4_2:16072452-16072471 Msa0591570:CDS 25.0%
! AATCAATTTATTAGAGTGAG+GGG + chr4_2:16072559-16072578 None:intergenic 25.0%
!!! TAGTATAAACTGACTTGATT+CGG - chr4_2:16072622-16072641 Msa0591570:intron 25.0%
! TTTCCATTTCTTTGATTAAC+AGG - chr4_2:16072705-16072724 Msa0591570:intron 25.0%
! TTTCCTGTTAATCAAAGAAA+TGG + chr4_2:16072711-16072730 None:intergenic 25.0%
! TGATTAACAGGAAATGAAAA+AGG - chr4_2:16072717-16072736 Msa0591570:intron 25.0%
! AGCTTTACTGAAGAATAATA+AGG + chr4_2:16073019-16073038 None:intergenic 25.0%
! TAGATAAAATTGGAATGGAA+CGG + chr4_2:16073217-16073236 None:intergenic 25.0%
! AGGTATAGATAAAATTGGAA+TGG + chr4_2:16073222-16073241 None:intergenic 25.0%
! CTACAACAATAATGAAAATG+AGG + chr4_2:16073711-16073730 None:intergenic 25.0%
! CATGTAAATCTAAGCAAAAA+AGG - chr4_2:16074067-16074086 Msa0591570:intron 25.0%
! AGAAAAACACTACATACATA+TGG + chr4_2:16074170-16074189 None:intergenic 25.0%
!! TATGTATGTAGTGTTTTTCT+TGG - chr4_2:16074170-16074189 Msa0591570:intron 25.0%
! TACAAGAATTATGTGACTAT+GGG + chr4_2:16074475-16074494 None:intergenic 25.0%
! TTACAAGAATTATGTGACTA+TGG + chr4_2:16074476-16074495 None:intergenic 25.0%
TAGTGAAGTGAATTATGAGA+AGG + chr4_2:16069968-16069987 None:intergenic 30.0%
! TACCTTTTGAACTTGAAACT+GGG - chr4_2:16070098-16070117 Msa0591570:three_prime_UTR 30.0%
!! GACTAAAAAGTGTTTCATGT+AGG - chr4_2:16070367-16070386 Msa0591570:intron 30.0%
AACAAATGATTGAGTTCAAG+CGG + chr4_2:16070442-16070461 None:intergenic 30.0%
TCATATATTTGATAGGTACG+TGG - chr4_2:16070531-16070550 Msa0591570:CDS 30.0%
CATATATTTGATAGGTACGT+GGG - chr4_2:16070532-16070551 Msa0591570:CDS 30.0%
ATATATTTGATAGGTACGTG+GGG - chr4_2:16070533-16070552 Msa0591570:CDS 30.0%
TTGGATATGTATAACACACA+TGG - chr4_2:16070759-16070778 Msa0591570:CDS 30.0%
! ATTCATTTTGCAGTGAAATG+TGG - chr4_2:16071067-16071086 Msa0591570:intron 30.0%
! TTCATTTTGCAGTGAAATGT+GGG - chr4_2:16071068-16071087 Msa0591570:intron 30.0%
GGGATACAACATAATAAATC+AGG + chr4_2:16071160-16071179 None:intergenic 30.0%
! GAATGAGAAGAACTATTTTG+TGG + chr4_2:16071360-16071379 None:intergenic 30.0%
ACAAGAAAATAGCCAAATCA+TGG + chr4_2:16071407-16071426 None:intergenic 30.0%
!! TTTGGCTATTTTCTTGTGTT+TGG - chr4_2:16071410-16071429 Msa0591570:intron 30.0%
! TCCAATTGCATTTGAAATCA+AGG - chr4_2:16071526-16071545 Msa0591570:intron 30.0%
TCCTTGATTTCAAATGCAAT+TGG + chr4_2:16071530-16071549 None:intergenic 30.0%
ATTATTGGAAGGTGATTTAC+CGG - chr4_2:16071786-16071805 Msa0591570:intron 30.0%
! ATTTTTGTAGATTTGCCACT+TGG - chr4_2:16071848-16071867 Msa0591570:intron 30.0%
CTCATATGCAAAAAATGTCA+CGG - chr4_2:16071880-16071899 Msa0591570:intron 30.0%
ACCATACTCTTCAATTTCTT+AGG - chr4_2:16071930-16071949 Msa0591570:CDS 30.0%
TCCTAAGAAATTGAAGAGTA+TGG + chr4_2:16071934-16071953 None:intergenic 30.0%
TACTCTTCAATTTCTTAGGA+AGG - chr4_2:16071934-16071953 Msa0591570:CDS 30.0%
GTAAGTTCATTATGCTAACT+AGG - chr4_2:16071956-16071975 Msa0591570:CDS 30.0%
AAAGAAAAAGTCTGAAGATG+TGG + chr4_2:16072019-16072038 None:intergenic 30.0%
TCGTAAATGAAGATCATAGT+CGG + chr4_2:16072168-16072187 None:intergenic 30.0%
TGATCTTCATTTACGAAAAC+TGG - chr4_2:16072172-16072191 Msa0591570:intron 30.0%
!! TCGAATTTTGAGTCATAGTT+AGG + chr4_2:16072276-16072295 None:intergenic 30.0%
AAATTTCTTGAAGAATGGCT+GGG + chr4_2:16072414-16072433 None:intergenic 30.0%
ATCAATTTATTAGAGTGAGG+GGG + chr4_2:16072558-16072577 None:intergenic 30.0%
TGATTATTTGCATTCTCCTA+TGG - chr4_2:16072574-16072593 Msa0591570:intron 30.0%
GAAACCTTGAGATTTATCAA+TGG + chr4_2:16072750-16072769 None:intergenic 30.0%
AATGCATATAACAAGATCGA+TGG + chr4_2:16072856-16072875 None:intergenic 30.0%
!! GAAAATATTGAGACTGGTTT+AGG + chr4_2:16072885-16072904 None:intergenic 30.0%
! AAACCAGTCTCAATATTTTC+AGG - chr4_2:16072885-16072904 Msa0591570:intron 30.0%
! AACCAGTCTCAATATTTTCA+GGG - chr4_2:16072886-16072905 Msa0591570:intron 30.0%
! TTTCTCTGTGTGTTGTTATA+GGG + chr4_2:16072927-16072946 None:intergenic 30.0%
!! TTTTCTCTGTGTGTTGTTAT+AGG + chr4_2:16072928-16072947 None:intergenic 30.0%
TATCAAATCCCATTTACTCA+TGG - chr4_2:16072952-16072971 Msa0591570:intron 30.0%
!! TGATGATGTGTTTTACTTGA+TGG - chr4_2:16073071-16073090 Msa0591570:intron 30.0%
TCTTAAACACAACTCATTTC+TGG + chr4_2:16073164-16073183 None:intergenic 30.0%
!! ATGATACTAAAGTTTTGGTG+TGG - chr4_2:16073251-16073270 Msa0591570:intron 30.0%
AATTACTTGGCAGTTTAGTT+CGG + chr4_2:16073626-16073645 None:intergenic 30.0%
!!! TTTTTTACCTCAGCACTAAA+TGG - chr4_2:16073738-16073757 Msa0591570:CDS 30.0%
! TTTTTACCTCAGCACTAAAT+GGG - chr4_2:16073739-16073758 Msa0591570:CDS 30.0%
TGAAAAATACCTCCGAAAAA+CGG + chr4_2:16074258-16074277 None:intergenic 30.0%
ACAAGAATTATGTGACTATG+GGG + chr4_2:16074474-16074493 None:intergenic 30.0%
TGAAGTGAATTATGAGAAGG+AGG + chr4_2:16069965-16069984 None:intergenic 35.0%
TATTGAAGCTTATGGCTCAA+AGG - chr4_2:16070045-16070064 Msa0591570:three_prime_UTR 35.0%
!! AACAACTTCCTGGTTTTCAA+GGG - chr4_2:16070071-16070090 Msa0591570:three_prime_UTR 35.0%
!! GTTTCAAGTTCAAAAGGTAG+TGG + chr4_2:16070097-16070116 None:intergenic 35.0%
! CTACCTTTTGAACTTGAAAC+TGG - chr4_2:16070097-16070116 Msa0591570:three_prime_UTR 35.0%
ATAAAGAATGACACTCCTCA+AGG - chr4_2:16070239-16070258 Msa0591570:CDS 35.0%
TCTTGCTAAACTAAACCTTG+AGG + chr4_2:16070257-16070276 None:intergenic 35.0%
CATAGTAATGCATAGTTCGT+CGG - chr4_2:16070329-16070348 Msa0591570:intron 35.0%
GTTTCATGTAGGATTCAACT+CGG - chr4_2:16070378-16070397 Msa0591570:intron 35.0%
TTTCATGTAGGATTCAACTC+GGG - chr4_2:16070379-16070398 Msa0591570:intron 35.0%
AGAAAGATCCTCTTATGCTT+TGG - chr4_2:16070620-16070639 Msa0591570:intron 35.0%
!! ACACACATGGATTGTTTTTC+TGG - chr4_2:16070772-16070791 Msa0591570:CDS 35.0%
CAGATGTTCAATTGTAGATG+TGG - chr4_2:16070951-16070970 Msa0591570:intron 35.0%
TCGAGATCATCATAGTCAAT+CGG + chr4_2:16071181-16071200 None:intergenic 35.0%
CTACCTAACCTTAGTTTAGT+CGG - chr4_2:16071218-16071237 Msa0591570:intron 35.0%
AAAAGTGACCGACTAAACTA+AGG + chr4_2:16071229-16071248 None:intergenic 35.0%
TCGATGTGATAACCATGATT+TGG - chr4_2:16071392-16071411 Msa0591570:CDS 35.0%
TTGAGTGGATCAATTTCAGT+AGG + chr4_2:16071459-16071478 None:intergenic 35.0%
ACTCTATGAATGTTGTTGAG+TGG + chr4_2:16071474-16071493 None:intergenic 35.0%
! TTTGAAATCAAGGAGTACGA+CGG - chr4_2:16071536-16071555 Msa0591570:intron 35.0%
CAAAAAATGTCACGGATCAT+CGG - chr4_2:16071888-16071907 Msa0591570:CDS 35.0%
! AACTATTCTCCATTTTCTCC+TGG + chr4_2:16072102-16072121 None:intergenic 35.0%
CGTGACAAGTAATATGACAA+CGG + chr4_2:16072207-16072226 None:intergenic 35.0%
GAAATTTCTTGAAGAATGGC+TGG + chr4_2:16072415-16072434 None:intergenic 35.0%
TTTGCTTTGCTTATTGCCAT+AGG + chr4_2:16072593-16072612 None:intergenic 35.0%
!!! TCTTTACGCTCTGGTTTTTA+GGG + chr4_2:16072666-16072685 None:intergenic 35.0%
CCTTGAGATTTATCAATGGT+TGG + chr4_2:16072746-16072765 None:intergenic 35.0%
CCAACCATTGATAAATCTCA+AGG - chr4_2:16072743-16072762 Msa0591570:CDS 35.0%
!!! CGATGGAACAAAGGTATTTT+GGG + chr4_2:16072839-16072858 None:intergenic 35.0%
!!! TCGATGGAACAAAGGTATTT+TGG + chr4_2:16072840-16072859 None:intergenic 35.0%
TAACAAGATCGATGGAACAA+AGG + chr4_2:16072848-16072867 None:intergenic 35.0%
ATCCCTGAAAATATTGAGAC+TGG + chr4_2:16072891-16072910 None:intergenic 35.0%
!! TATTTTCAGGGATATCTGCT+AGG - chr4_2:16072898-16072917 Msa0591570:intron 35.0%
!! ATTTTCAGGGATATCTGCTA+GGG - chr4_2:16072899-16072918 Msa0591570:intron 35.0%
AATCCCATTTACTCATGGAA+TGG - chr4_2:16072957-16072976 Msa0591570:intron 35.0%
ATCCCATTTACTCATGGAAT+GGG - chr4_2:16072958-16072977 Msa0591570:intron 35.0%
TTCCCATTCCATGAGTAAAT+GGG + chr4_2:16072963-16072982 None:intergenic 35.0%
ATTCCCATTCCATGAGTAAA+TGG + chr4_2:16072964-16072983 None:intergenic 35.0%
CATACATTGTACTTTCCTGT+TGG - chr4_2:16073042-16073061 Msa0591570:intron 35.0%
ACATCATCATTGTAACCAAC+AGG + chr4_2:16073060-16073079 None:intergenic 35.0%
GATGGTCTACTGTTATCTAT+TGG - chr4_2:16073089-16073108 Msa0591570:intron 35.0%
CAACAGTAGCTACTTAATCA+TGG - chr4_2:16073296-16073315 Msa0591570:intron 35.0%
! ACTTGATCTGTTCCACATTT+TGG - chr4_2:16073513-16073532 Msa0591570:intron 35.0%
! ATTACTTGGCAGTTTAGTTC+GGG + chr4_2:16073625-16073644 None:intergenic 35.0%
!! GATTTTGAGAATGAGATCCT+TGG - chr4_2:16074031-16074050 Msa0591570:intron 35.0%
TTCACCTGTAAATCAAAGAG+CGG + chr4_2:16074096-16074115 None:intergenic 35.0%
!! AGTGAGCATTTTGCTTTAGA+AGG - chr4_2:16074208-16074227 Msa0591570:CDS 35.0%
!!! ATTTTGCTTTAGAAGGTGGT+CGG - chr4_2:16074215-16074234 Msa0591570:CDS 35.0%
!! AAGTCTATTGCACCGTTTTT+CGG - chr4_2:16074243-16074262 Msa0591570:CDS 35.0%
!!! TTCGGAGGTATTTTTCACTT+TGG - chr4_2:16074261-16074280 Msa0591570:CDS 35.0%
!!! TCGGAGGTATTTTTCACTTT+GGG - chr4_2:16074262-16074281 Msa0591570:CDS 35.0%
AAAATAGCTAGTAAGCCCTT+AGG + chr4_2:16074437-16074456 None:intergenic 35.0%
!!! GGCTTACTAGCTATTTTTTG+TGG - chr4_2:16074440-16074459 Msa0591570:five_prime_UTR 35.0%
!!! CTAGCTATTTTTTGTGGCTT+TGG - chr4_2:16074446-16074465 Msa0591570:five_prime_UTR 35.0%
CAAGAATTATGTGACTATGG+GGG + chr4_2:16074473-16074492 None:intergenic 35.0%
GCTTGCAAAGATGAACATGA+TGG - chr4_2:16069937-16069956 Msa0591570:three_prime_UTR 40.0%
! TGTCAGATACTACTAGCTCT+TGG - chr4_2:16069995-16070014 Msa0591570:three_prime_UTR 40.0%
TCAAAGGTTGAACAACTTCC+TGG - chr4_2:16070061-16070080 Msa0591570:three_prime_UTR 40.0%
!! GAACAACTTCCTGGTTTTCA+AGG - chr4_2:16070070-16070089 Msa0591570:three_prime_UTR 40.0%
!! GACCCAGTTTCAAGTTCAAA+AGG + chr4_2:16070103-16070122 None:intergenic 40.0%
AGCATAAGAGGATCTTTCTG+AGG + chr4_2:16070619-16070638 None:intergenic 40.0%
CCAGTTAACCAAAGCATAAG+AGG + chr4_2:16070631-16070650 None:intergenic 40.0%
CCTCTTATGCTTTGGTTAAC+TGG - chr4_2:16070628-16070647 Msa0591570:intron 40.0%
CTTATGCTTTGGTTAACTGG+TGG - chr4_2:16070631-16070650 Msa0591570:intron 40.0%
TCTGGACTTGCCTATCAAAT+AGG - chr4_2:16070673-16070692 Msa0591570:intron 40.0%
TTTGTGAGTCGAATTACGAG+AGG - chr4_2:16070850-16070869 Msa0591570:intron 40.0%
ATTGTAGATGTGGCTGCATT+TGG - chr4_2:16070961-16070980 Msa0591570:intron 40.0%
CGAGATCATCATAGTCAATC+GGG + chr4_2:16071180-16071199 None:intergenic 40.0%
TGACCGACTAAACTAAGGTT+AGG + chr4_2:16071224-16071243 None:intergenic 40.0%
! TTGAAATCAAGGAGTACGAC+GGG - chr4_2:16071537-16071556 Msa0591570:intron 40.0%
TATGAGAAACCAGTTCCAAG+TGG + chr4_2:16071866-16071885 None:intergenic 40.0%
ATGTCTTGTGCATAGAGTTG+CGG + chr4_2:16072068-16072087 None:intergenic 40.0%
! ATTGGAGCTGATTCATACTC+AGG - chr4_2:16072379-16072398 Msa0591570:CDS 40.0%
!!! CTTTACGCTCTGGTTTTTAG+GGG + chr4_2:16072665-16072684 None:intergenic 40.0%
!!! GTCTTTACGCTCTGGTTTTT+AGG + chr4_2:16072667-16072686 None:intergenic 40.0%
!!! GTTTTGAAGTCTTTACGCTC+TGG + chr4_2:16072675-16072694 None:intergenic 40.0%
GAAGCAACTGATAGAGATGA+TGG + chr4_2:16072776-16072795 None:intergenic 40.0%
CATTTCTGATGAACTCTACG+CGG - chr4_2:16072984-16073003 Msa0591570:intron 40.0%
TACTTGCCTCATCATAGGAT+CGG + chr4_2:16073192-16073211 None:intergenic 40.0%
!! AAAGTTTTGGTGTGGTCTTG+AGG - chr4_2:16073259-16073278 Msa0591570:intron 40.0%
AAGGGAAAAAAAGGAGAGAC+TGG - chr4_2:16073388-16073407 Msa0591570:intron 40.0%
AAAGGAGAGACTGGTAAAGA+TGG - chr4_2:16073397-16073416 Msa0591570:intron 40.0%
TCTTATCATCAGTGCCTTTC+AGG - chr4_2:16073490-16073509 Msa0591570:intron 40.0%
CACAAAAGCGATCCAAAATG+TGG + chr4_2:16073528-16073547 None:intergenic 40.0%
ATCTTTGACTCAGGAGTTGA+AGG - chr4_2:16073573-16073592 Msa0591570:intron 40.0%
CAAAGAAGTCTCAACAACTC+AGG - chr4_2:16073930-16073949 Msa0591570:intron 40.0%
!! TACATAGCTTTGAGCATTGC+AGG - chr4_2:16073973-16073992 Msa0591570:intron 40.0%
!! ACATAGCTTTGAGCATTGCA+GGG - chr4_2:16073974-16073993 Msa0591570:intron 40.0%
CATGAAACTCAAAGCTACCA+AGG + chr4_2:16074051-16074070 None:intergenic 40.0%
! GATACCGCTCTTTGATTTAC+AGG - chr4_2:16074089-16074108 Msa0591570:intron 40.0%
!!! GAGCATTTTGCTTTAGAAGG+TGG - chr4_2:16074211-16074230 Msa0591570:CDS 40.0%
CGAAGCAATGTGAGATTTCT+TGG - chr4_2:16074374-16074393 Msa0591570:exon 40.0%
! TGTGAGATTTCTTGGTGTAC+TGG - chr4_2:16074382-16074401 Msa0591570:five_prime_UTR 40.0%
AGTAATGCATAGTTCGTCGG+TGG - chr4_2:16070332-16070351 Msa0591570:intron 45.0%
TAGTTCGTCGGTGGTAAGTA+AGG - chr4_2:16070341-16070360 Msa0591570:intron 45.0%
TTTGATAGGTACGTGGGGTT+AGG - chr4_2:16070538-16070557 Msa0591570:CDS 45.0%
TTGATAGGTACGTGGGGTTA+GGG - chr4_2:16070539-16070558 Msa0591570:CDS 45.0%
CCAGAAAAAGAAGAACAGCC+AGG + chr4_2:16070658-16070677 None:intergenic 45.0%
! CCTGGCTGTTCTTCTTTTTC+TGG - chr4_2:16070655-16070674 Msa0591570:intron 45.0%
AACACCTTTGTCCATGACTG+TGG + chr4_2:16071587-16071606 None:intergenic 45.0%
! GTAGATTTGCCACTTGGAAC+TGG - chr4_2:16071854-16071873 Msa0591570:intron 45.0%
TATGCTAACTAGGGTGCAGA+AGG - chr4_2:16071966-16071985 Msa0591570:intron 45.0%
ATGCTAACTAGGGTGCAGAA+GGG - chr4_2:16071967-16071986 Msa0591570:intron 45.0%
AGATCTCCGATCCTATGATG+AGG - chr4_2:16073183-16073202 Msa0591570:intron 45.0%
CGGTTTACTTGCCTCATCAT+AGG + chr4_2:16073197-16073216 None:intergenic 45.0%
GGAACAGATCAAGTCCTGAA+AGG + chr4_2:16073507-16073526 None:intergenic 45.0%
AAAGATCTTCTCCACAGACG+CGG + chr4_2:16073559-16073578 None:intergenic 45.0%
GTGGAGAAGATCTTTGACTC+AGG - chr4_2:16073564-16073583 Msa0591570:intron 45.0%
TGTTAGCCCATTTAGTGCTG+AGG + chr4_2:16073748-16073767 None:intergenic 45.0%
TAACAATGAGCGTGTTCGCA+AGG - chr4_2:16073763-16073782 Msa0591570:intron 45.0%
!! TCTATTGCACCGTTTTTCGG+AGG - chr4_2:16074246-16074265 Msa0591570:CDS 45.0%
AACTAATGGCGAAACCGCTA+GGG + chr4_2:16074299-16074318 None:intergenic 45.0%
! CCAAGGCGTCTTTCAACTAA+TGG + chr4_2:16074313-16074332 None:intergenic 45.0%
CCATTAGTTGAAAGACGCCT+TGG - chr4_2:16074310-16074329 Msa0591570:CDS 45.0%
TTGAAAGACGCCTTGGTTAG+TGG - chr4_2:16074317-16074336 Msa0591570:CDS 45.0%
ACAAACCACTCCACTAACCA+AGG + chr4_2:16074330-16074349 None:intergenic 45.0%
!!! AATCTTATTATATATTTTTT+TGG + chr4_2:16071326-16071345 None:intergenic 5.0%
TGATAGGTACGTGGGGTTAG+GGG - chr4_2:16070540-16070559 Msa0591570:CDS 50.0%
CTTTGGTTAACTGGTGGTCC+TGG - chr4_2:16070637-16070656 Msa0591570:intron 50.0%
TGTGGCCTCAAGACCAAACT+AGG + chr4_2:16071569-16071588 None:intergenic 50.0%
!! AAGACATACAAGAGCGCTCC+AGG - chr4_2:16072081-16072100 Msa0591570:intron 50.0%
!! AAGAGCGCTCCAGGAGAAAA+TGG - chr4_2:16072090-16072109 Msa0591570:intron 50.0%
TCTTGAAGAATGGCTGGGAC+AGG + chr4_2:16072409-16072428 None:intergenic 50.0%
!!! GCTCTGGTTTTTAGGGGAAG+GGG + chr4_2:16072659-16072678 None:intergenic 50.0%
!!! CGCTCTGGTTTTTAGGGGAA+GGG + chr4_2:16072660-16072679 None:intergenic 50.0%
!!! ACGCTCTGGTTTTTAGGGGA+AGG + chr4_2:16072661-16072680 None:intergenic 50.0%
!! CAAGGCACTGCATATTCGAG+AGG - chr4_2:16073781-16073800 Msa0591570:intron 50.0%
! TGAGCATTGCAGGGAACCAT+AGG - chr4_2:16073983-16074002 Msa0591570:intron 50.0%
! GAGCATTGCAGGGAACCATA+GGG - chr4_2:16073984-16074003 Msa0591570:intron 50.0%
TGCAGGGAACCATAGGGAAA+TGG - chr4_2:16073990-16074009 Msa0591570:intron 50.0%
GCAGGGAACCATAGGGAAAT+GGG - chr4_2:16073991-16074010 Msa0591570:intron 50.0%
ACAGCGTTCCCATTTCCCTA+TGG + chr4_2:16074002-16074021 None:intergenic 50.0%
CAACTAATGGCGAAACCGCT+AGG + chr4_2:16074300-16074319 None:intergenic 50.0%
! GGTAGTGGCCCTTGAAAACC+AGG + chr4_2:16070082-16070101 None:intergenic 55.0%
!! GCGTGCCTAGTTTGGTCTTG+AGG - chr4_2:16071561-16071580 Msa0591570:CDS 55.0%
! TGGTCTTGAGGCCACAGTCA+TGG - chr4_2:16071573-16071592 Msa0591570:CDS 55.0%
GAGGCCACAGTCATGGACAA+AGG - chr4_2:16071580-16071599 Msa0591570:intron 55.0%
! GGGTCTTGTGTAAGCCCTAG+CGG - chr4_2:16074282-16074301 Msa0591570:CDS 55.0%
AGACGCCTTGGTTAGTGGAG+TGG - chr4_2:16074322-16074341 Msa0591570:CDS 55.0%
CTGGAACAGTAGCCCCAAGT+CGG - chr4_2:16074401-16074420 Msa0591570:five_prime_UTR 55.0%
TGGAACAGTAGCCCCAAGTC+GGG - chr4_2:16074402-16074421 Msa0591570:five_prime_UTR 55.0%
TCACGGATCATCGGAGCGAC+TGG - chr4_2:16071897-16071916 Msa0591570:CDS 60.0%
GTGACGATGATCCGCGTCTG+TGG - chr4_2:16073545-16073564 Msa0591570:intron 60.0%
GGAACAGTAGCCCCAAGTCG+GGG - chr4_2:16074403-16074422 Msa0591570:five_prime_UTR 60.0%
TAGCCCCAAGTCGGGGCTAA+GGG - chr4_2:16074410-16074429 Msa0591570:five_prime_UTR 60.0%
! CGACGGGAGCGTGCCTAGTT+TGG - chr4_2:16071553-16071572 Msa0591570:intron 65.0%
GTAGCCCCAAGTCGGGGCTA+AGG - chr4_2:16074409-16074428 Msa0591570:five_prime_UTR 65.0%
AGCCCCAAGTCGGGGCTAAG+GGG - chr4_2:16074411-16074430 Msa0591570:five_prime_UTR 65.0%
AGGCCCCTTAGCCCCGACTT+GGG + chr4_2:16074417-16074436 None:intergenic 65.0%
TAGGCCCCTTAGCCCCGACT+TGG + chr4_2:16074418-16074437 None:intergenic 65.0%
AGTCGGGGCTAAGGGGCCTA+AGG - chr4_2:16074418-16074437 Msa0591570:five_prime_UTR 65.0%
GTCGGGGCTAAGGGGCCTAA+GGG - chr4_2:16074419-16074438 Msa0591570:five_prime_UTR 65.0%
! GGCCCCTTAGCCCCGACTTG+GGG + chr4_2:16074416-16074435 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 16069821 16074500 16069821 ID=Msa0591570;Name=Msa0591570
chr4_2 mRNA 16069821 16074500 16069821 ID=Msa0591570-mRNA-1;Parent=Msa0591570;Name=Msa0591570-mRNA-1;_AED=0.27;_eAED=0.27;_QI=126|1|1|1|1|1|11|386|391
chr4_2 exon 16069821 16070326 16069821 ID=Msa0591570-mRNA-1:exon:4639;Parent=Msa0591570-mRNA-1
chr4_2 exon 16070519 16070590 16070519 ID=Msa0591570-mRNA-1:exon:4638;Parent=Msa0591570-mRNA-1
chr4_2 exon 16070682 16070819 16070682 ID=Msa0591570-mRNA-1:exon:4637;Parent=Msa0591570-mRNA-1
chr4_2 exon 16071117 16071203 16071117 ID=Msa0591570-mRNA-1:exon:4636;Parent=Msa0591570-mRNA-1
chr4_2 exon 16071316 16071414 16071316 ID=Msa0591570-mRNA-1:exon:4635;Parent=Msa0591570-mRNA-1
chr4_2 exon 16071557 16071594 16071557 ID=Msa0591570-mRNA-1:exon:4634;Parent=Msa0591570-mRNA-1
chr4_2 exon 16071882 16071984 16071882 ID=Msa0591570-mRNA-1:exon:4633;Parent=Msa0591570-mRNA-1
chr4_2 exon 16072366 16072485 16072366 ID=Msa0591570-mRNA-1:exon:4632;Parent=Msa0591570-mRNA-1
chr4_2 exon 16072720 16072796 16072720 ID=Msa0591570-mRNA-1:exon:4631;Parent=Msa0591570-mRNA-1
chr4_2 exon 16073627 16073775 16073627 ID=Msa0591570-mRNA-1:exon:4630;Parent=Msa0591570-mRNA-1
chr4_2 exon 16074202 16074500 16074202 ID=Msa0591570-mRNA-1:exon:4629;Parent=Msa0591570-mRNA-1
chr4_2 five_prime_UTR 16074375 16074500 16074375 ID=Msa0591570-mRNA-1:five_prime_utr;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16074202 16074374 16074202 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16073627 16073775 16073627 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16072720 16072796 16072720 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16072366 16072485 16072366 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16071882 16071984 16071882 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16071557 16071594 16071557 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16071316 16071414 16071316 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16071117 16071203 16071117 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16070682 16070819 16070682 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16070519 16070590 16070519 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 CDS 16070207 16070326 16070207 ID=Msa0591570-mRNA-1:cds;Parent=Msa0591570-mRNA-1
chr4_2 three_prime_UTR 16069821 16070206 16069821 ID=Msa0591570-mRNA-1:three_prime_utr;Parent=Msa0591570-mRNA-1
Gene Sequence

>Msa0591570

ATGAACATGATGGCTCCTCCTTCTCATAATTCACTTCACTATTGTTGTTGTCAGATACTACTAGCTCTTGGACTATTTAGTTTGAATATGTTAACTCATATTGAAGCTTATGGCTCAAAGGTTGAACAACTTCCTGGTTTTCAAGGGCCACTACCTTTTGAACTTGAAACTGGGTACGTGGGGTTAGGGGAAACAGATGATGACATGCAAGTATTCTACTATTTTATCAAATCAGAAAATGATCCTCAGAAAGATCCTCTTATGCTTTGGTTAACTGGTGGTCCTGGCTGTTCTTCTTTTTCTGGACTTGCCTATCAAATAGGTCCAATTGCATTTGAAATCAAGGAGTACGACGGGAGCGTGCCTAGTTTGGTCTTGAGGCCACAGTCATGGACAAAGCTATGCAGCATTATTTTTGTAGATTTGCCACTTGGAACTGGTTTCTCATATGCAAAAAATGTCACGGATCATCGGAGCGACTGGAAACTAGTTCACCATACTCTTCAATTTCTTAGGAAGTGGCTGATCGATCACTCTGAATTTCTTTCAAATGAATTCTACATTGGAGCTGATTCATACTCAGGCATTCCTGTCCCAGCCATTCTTCAAGAAATTTCAAATGGAAATGAAAAAGGTCTCCAACCATTGATAAATCTCAAGGGATATCTGCTAGGGAACCCTATAACAACACACAGAGAAAAAAATTATCAAATCCCATTTACTCATGGAATGGGAATCATTTCTGATGAACTCTACGCGTCACTGCAGAGAAACTGTAAAAGAGAGTATGTTGATGTAGATTCCAGAAATGAGTTGTGTTTAAGAGATCTCCGATCCTATGATGAGTGCCTTTCAGGACTTGATCTGTTCCACATTTTGGATCGCTTTTGTGACGATGATCCGCGTCTGTGGAGAAGATCTTTGACTCAGGAGTTGAAGGAATCTCTTAGTTCTCATCTCACAGTGCCCGAACTAAACTGCCAATTTTATATTTTTTACCTCAGCACTAAATGGGCTAACAATGAGCGTGTTCGCAAGGCACTGCATATTCGAGAGGGAACCATAGGGAAATGGGAACGCTGTTACTCTAATGATTTTGAGAATGAGATCCTTGGTAGCTTTGAGTTTCATGTAAATCTAAGCAAAAAAGGATACCGCTCTTTGATTTACAGGTGA
Protein sequence

>Msa0591570

MNMMAPPSHNSLHYCCCQILLALGLFSLNMLTHIEAYGSKVEQLPGFQGPLPFELETGYVGLGETDDDMQVFYYFIKSENDPQKDPLMLWLTGGPGCSSFSGLAYQIGPIAFEIKEYDGSVPSLVLRPQSWTKLCSIIFVDLPLGTGFSYAKNVTDHRSDWKLVHHTLQFLRKWLIDHSEFLSNEFYIGADSYSGIPVPAILQEISNGNEKGLQPLINLKGYLLGNPITTHREKNYQIPFTHGMGIISDELYASLQRNCKREYVDVDSRNELCLRDLRSYDECLSGLDLFHILDRFCDDDPRLWRRSLTQELKESLSSHLTVPELNCQFYIFYLSTKWANNERVRKALHIREGTIGKWERCYSNDFENEILGSFEFHVNLSKKGYRSLIYR*