Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483000 | XP_024636960.1 | 100.000 | 77 | 0 | 0 | 1 | 77 | 1 | 77 | 8.50e-47 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483000 | sp|O82232|RC22_ARATH | 79.167 | 72 | 15 | 0 | 1 | 72 | 1 | 72 | 6.74e-37 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483000 | I3S6D4 | 100.000 | 77 | 0 | 0 | 1 | 77 | 1 | 77 | 4.06e-47 | 153 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030800 | Msa0483000 | 0.813953 | 1.971451e-51 | -8.615850e-47 |
| Msa0033970 | Msa0483000 | 0.805471 | 1.314049e-49 | -8.615850e-47 |
| Msa0076640 | Msa0483000 | 0.806060 | 9.881468e-50 | -8.615850e-47 |
| Msa0078210 | Msa0483000 | 0.805117 | 1.558643e-49 | -8.615850e-47 |
| Msa0081920 | Msa0483000 | 0.804676 | 1.926961e-49 | -8.615850e-47 |
| Msa0095910 | Msa0483000 | 0.807842 | 4.147730e-50 | -8.615850e-47 |
| Msa0107830 | Msa0483000 | 0.801560 | 8.498998e-49 | -8.615850e-47 |
| Msa0128030 | Msa0483000 | 0.818744 | 1.670634e-52 | -8.615850e-47 |
| Msa0143640 | Msa0483000 | 0.819753 | 9.842537e-53 | -8.615850e-47 |
| Msa0186240 | Msa0483000 | 0.829504 | 4.961001e-55 | -8.615850e-47 |
| Msa0191470 | Msa0483000 | 0.820064 | 8.355861e-53 | -8.615850e-47 |
| Msa0200090 | Msa0483000 | 0.802180 | 6.340434e-49 | -8.615850e-47 |
| Msa0224210 | Msa0483000 | 0.800682 | 1.284963e-48 | -8.615850e-47 |
| Msa0238140 | Msa0483000 | 0.815322 | 9.811951e-52 | -8.615850e-47 |
| Msa0238330 | Msa0483000 | 0.804336 | 2.268377e-49 | -8.615850e-47 |
| Msa0368620 | Msa0483000 | 0.811401 | 7.130111e-51 | -8.615850e-47 |
| Msa0389710 | Msa0483000 | 0.813227 | 2.848352e-51 | -8.615850e-47 |
| Msa0390790 | Msa0483000 | 0.802488 | 5.477458e-49 | -8.615850e-47 |
| Msa0402800 | Msa0483000 | 0.817917 | 2.570954e-52 | -8.615850e-47 |
| Msa0405200 | Msa0483000 | 0.816133 | 6.469956e-52 | -8.615850e-47 |
| Msa0421080 | Msa0483000 | 0.830381 | 3.031839e-55 | -8.615850e-47 |
| Msa0427560 | Msa0483000 | 0.805342 | 1.398269e-49 | -8.615850e-47 |
| Msa0447160 | Msa0483000 | 0.820276 | 7.472124e-53 | -8.615850e-47 |
| Msa0449500 | Msa0483000 | 0.825577 | 4.346316e-54 | -8.615850e-47 |
| Msa0463890 | Msa0483000 | 0.814165 | 1.770528e-51 | -8.615850e-47 |
| Msa0478080 | Msa0483000 | 0.807700 | 4.446167e-50 | -8.615850e-47 |
| Msa0480260 | Msa0483000 | 0.811227 | 7.779677e-51 | -8.615850e-47 |
| Msa0480530 | Msa0483000 | 0.801823 | 7.507367e-49 | -8.615850e-47 |
| Msa0480540 | Msa0483000 | 0.830430 | 2.949663e-55 | -8.615850e-47 |
| Msa0480550 | Msa0483000 | 0.842720 | 2.176414e-58 | -8.615850e-47 |
| Msa0481770 | Msa0483000 | 0.826896 | 2.108667e-54 | -8.615850e-47 |
| Msa0246140 | Msa0483000 | 0.829883 | 4.012348e-55 | -8.615850e-47 |
| Msa0259770 | Msa0483000 | -0.804102 | 2.538275e-49 | -8.615850e-47 |
| Msa0262360 | Msa0483000 | 0.804412 | 2.187299e-49 | -8.615850e-47 |
| Msa0267700 | Msa0483000 | 0.825888 | 3.666967e-54 | -8.615850e-47 |
| Msa0267900 | Msa0483000 | 0.826565 | 2.529797e-54 | -8.615850e-47 |
| Msa0269710 | Msa0483000 | 0.819192 | 1.321348e-52 | -8.615850e-47 |
| Msa0271700 | Msa0483000 | 0.827791 | 1.286906e-54 | -8.615850e-47 |
| Msa0271720 | Msa0483000 | 0.819570 | 1.083794e-52 | -8.615850e-47 |
| Msa0275730 | Msa0483000 | 0.808201 | 3.479414e-50 | -8.615850e-47 |
| Msa0276780 | Msa0483000 | 0.804145 | 2.485584e-49 | -8.615850e-47 |
| Msa0308740 | Msa0483000 | 0.802326 | 5.917388e-49 | -8.615850e-47 |
| Msa0310260 | Msa0483000 | -0.803309 | 3.706608e-49 | -8.615850e-47 |
| Msa0312300 | Msa0483000 | 0.832456 | 9.357908e-56 | -8.615850e-47 |
| Msa0316230 | Msa0483000 | 0.808074 | 3.702256e-50 | -8.615850e-47 |
| Msa0324810 | Msa0483000 | 0.855955 | 4.455808e-62 | -8.615850e-47 |
| Msa0332360 | Msa0483000 | 0.811809 | 5.813304e-51 | -8.615850e-47 |
| Msa0353010 | Msa0483000 | 0.805717 | 1.166347e-49 | -8.615850e-47 |
| Msa0483000 | Msa0493870 | 0.826723 | 2.319816e-54 | -8.615850e-47 |
| Msa0483000 | Msa0497680 | 0.823799 | 1.139940e-53 | -8.615850e-47 |
| Msa0483000 | Msa0497860 | 0.811606 | 6.436486e-51 | -8.615850e-47 |
| Msa0483000 | Msa0508160 | 0.820767 | 5.763996e-53 | -8.615850e-47 |
| Msa0483000 | Msa0510870 | 0.801038 | 1.087128e-48 | -8.615850e-47 |
| Msa0483000 | Msa0522140 | 0.804150 | 2.480338e-49 | -8.615850e-47 |
| Msa0483000 | Msa0522170 | 0.825837 | 3.770290e-54 | -8.615850e-47 |
| Msa0483000 | Msa0531050 | 0.800810 | 1.210362e-48 | -8.615850e-47 |
| Msa0483000 | Msa0548790 | 0.818326 | 2.078381e-52 | -8.615850e-47 |
| Msa0483000 | Msa0550760 | -0.806280 | 8.880868e-50 | -8.615850e-47 |
| Msa0483000 | Msa0565180 | 0.819514 | 1.115687e-52 | -8.615850e-47 |
| Msa0483000 | Msa0570460 | 0.803019 | 4.257465e-49 | -8.615850e-47 |
| Msa0483000 | Msa0572700 | 0.816927 | 4.296119e-52 | -8.615850e-47 |
| Msa0483000 | Msa0580840 | 0.811659 | 6.267870e-51 | -8.615850e-47 |
| Msa0483000 | Msa0588040 | 0.807033 | 6.159683e-50 | -8.615850e-47 |
| Msa0483000 | Msa0594470 | 0.804397 | 2.203187e-49 | -8.615850e-47 |
| Msa0483000 | Msa0628200 | 0.809457 | 1.874705e-50 | -8.615850e-47 |
| Msa0483000 | Msa0638820 | 0.803554 | 3.298700e-49 | -8.615850e-47 |
| Msa0483000 | Msa0651820 | 0.826000 | 3.449223e-54 | -8.615850e-47 |
| Msa0483000 | Msa0657260 | 0.825032 | 5.845669e-54 | -8.615850e-47 |
| Msa0483000 | Msa0657340 | 0.803336 | 3.659950e-49 | -8.615850e-47 |
| Msa0483000 | Msa0675630 | 0.805892 | 1.071714e-49 | -8.615850e-47 |
| Msa0483000 | Msa0697510 | 0.802850 | 4.613812e-49 | -8.615850e-47 |
| Msa0483000 | Msa0697520 | 0.813634 | 2.317165e-51 | -8.615850e-47 |
| Msa0483000 | Msa0701430 | 0.818270 | 2.139122e-52 | -8.615850e-47 |
| Msa0483000 | Msa0701490 | 0.846713 | 1.827409e-59 | -8.615850e-47 |
| Msa0483000 | Msa0701560 | 0.842489 | 2.507246e-58 | -8.615850e-47 |
| Msa0483000 | Msa0704560 | 0.805870 | 1.083398e-49 | -8.615850e-47 |
| Msa0483000 | Msa0716620 | 0.804976 | 1.668217e-49 | -8.615850e-47 |
| Msa0483000 | Msa0721260 | 0.814205 | 1.734824e-51 | -8.615850e-47 |
| Msa0483000 | Msa0727230 | 0.825125 | 5.559198e-54 | -8.615850e-47 |
| Msa0483000 | Msa0744550 | 0.833740 | 4.483179e-56 | -8.615850e-47 |
| Msa0483000 | Msa0744560 | 0.844648 | 6.636349e-59 | -8.615850e-47 |
| Msa0483000 | Msa0751510 | 0.805318 | 1.414338e-49 | -8.615850e-47 |
| Msa0483000 | Msa0753410 | 0.838684 | 2.484881e-57 | -8.615850e-47 |
| Msa0483000 | Msa0754530 | 0.807941 | 3.952014e-50 | -8.615850e-47 |
| Msa0483000 | Msa0794200 | 0.816466 | 5.452020e-52 | -8.615850e-47 |
| Msa0483000 | Msa0795320 | 0.849074 | 4.082718e-60 | -8.615850e-47 |
| Msa0483000 | Msa0795330 | 0.822327 | 2.513461e-53 | -8.615850e-47 |
| Msa0483000 | Msa0826360 | 0.852312 | 5.014726e-61 | -8.615850e-47 |
| Msa0483000 | Msa0826370 | 0.826664 | 2.395893e-54 | -8.615850e-47 |
| Msa0483000 | Msa0826380 | 0.811606 | 6.436467e-51 | -8.615850e-47 |
| Msa0483000 | Msa0826450 | 0.808570 | 2.902007e-50 | -8.615850e-47 |
| Msa0483000 | Msa0829990 | 0.805224 | 1.480258e-49 | -8.615850e-47 |
| Msa0483000 | Msa0833230 | 0.803909 | 2.783480e-49 | -8.615850e-47 |
| Msa0483000 | Msa0841030 | 0.807447 | 5.033255e-50 | -8.615850e-47 |
| Msa0483000 | Msa0855260 | 0.800163 | 1.639561e-48 | -8.615850e-47 |
| Msa0483000 | Msa0865810 | 0.819039 | 1.431376e-52 | -8.615850e-47 |
| Msa0483000 | Msa0865820 | 0.820689 | 6.007192e-53 | -8.615850e-47 |
| Msa0483000 | Msa0865830 | 0.847776 | 9.335368e-60 | -8.615850e-47 |
| Msa0483000 | Msa0921790 | 0.801529 | 8.623718e-49 | -8.615850e-47 |
| Msa0483000 | Msa0947420 | 0.810313 | 1.226421e-50 | -8.615850e-47 |
| Msa0483000 | Msa0947520 | 0.811468 | 6.896275e-51 | -8.615850e-47 |
| Msa0483000 | Msa0960580 | 0.805748 | 1.149380e-49 | -8.615850e-47 |
| Msa0483000 | Msa1037360 | 0.830346 | 3.092272e-55 | -8.615850e-47 |
| Msa0483000 | Msa1061840 | 0.806575 | 7.697994e-50 | -8.615850e-47 |
| Msa0483000 | Msa1071350 | 0.809600 | 1.746435e-50 | -8.615850e-47 |
| Msa0483000 | Msa1075090 | 0.808026 | 3.790639e-50 | -8.615850e-47 |
| Msa0483000 | Msa1087210 | 0.821208 | 4.563206e-53 | -8.615850e-47 |
| Msa0483000 | Msa1107220 | 0.805332 | 1.405007e-49 | -8.615850e-47 |
| Msa0483000 | Msa1107250 | 0.814013 | 1.912518e-51 | -8.615850e-47 |
| Msa0483000 | Msa1123000 | 0.816691 | 4.854405e-52 | -8.615850e-47 |
| Msa0483000 | Msa1131430 | 0.823880 | 1.091415e-53 | -8.615850e-47 |
| Msa0483000 | Msa1137750 | 0.801322 | 9.512153e-49 | -8.615850e-47 |
| Msa0483000 | Msa1150740 | 0.802846 | 4.622516e-49 | -8.615850e-47 |
| Msa0483000 | Msa1150760 | 0.802680 | 5.000284e-49 | -8.615850e-47 |
| Msa0483000 | Msa1157200 | 0.858080 | 1.053091e-62 | -8.615850e-47 |
| Msa0483000 | Msa1164230 | 0.811782 | 5.894474e-51 | -8.615850e-47 |
| Msa0483000 | Msa1165670 | 0.814883 | 1.227795e-51 | -8.615850e-47 |
| Msa0483000 | Msa1174450 | 0.801989 | 6.940246e-49 | -8.615850e-47 |
| Msa0483000 | Msa1185270 | 0.800662 | 1.297393e-48 | -8.615850e-47 |
| Msa0483000 | Msa1202110 | 0.825254 | 5.180603e-54 | -8.615850e-47 |
| Msa0483000 | Msa1211610 | 0.814092 | 1.836982e-51 | -8.615850e-47 |
| Msa0483000 | Msa1217260 | 0.800956 | 1.129746e-48 | -8.615850e-47 |
| Msa0483000 | Msa1239190 | 0.803695 | 3.083743e-49 | -8.615850e-47 |
| Msa0483000 | Msa1254870 | 0.826276 | 2.965398e-54 | -8.615850e-47 |
| Msa0483000 | Msa1269200 | 0.803768 | 2.978144e-49 | -8.615850e-47 |
| Msa0483000 | Msa1292560 | 0.811577 | 6.531647e-51 | -8.615850e-47 |
| Msa0483000 | Msa1299480 | 0.810562 | 1.083779e-50 | -8.615850e-47 |
| Msa0483000 | Msa1302340 | 0.812481 | 4.149753e-51 | -8.615850e-47 |
| Msa0483000 | Msa1328430 | 0.825813 | 3.819974e-54 | -8.615850e-47 |
| Msa0483000 | Msa1349830 | 0.833739 | 4.486822e-56 | -8.615850e-47 |
| Msa0483000 | Msa1361710 | 0.818243 | 2.170247e-52 | -8.615850e-47 |
| Msa0483000 | Msa1362630 | 0.810682 | 1.020787e-50 | -8.615850e-47 |
| Msa0483000 | Msa1366480 | 0.814045 | 1.881824e-51 | -8.615850e-47 |
| Msa0483000 | Msa1372470 | -0.807093 | 5.981954e-50 | -8.615850e-47 |
| Msa0483000 | Msa1375640 | 0.818136 | 2.294727e-52 | -8.615850e-47 |
| Msa0483000 | Msa1376520 | 0.816418 | 5.587926e-52 | -8.615850e-47 |
| Msa0483000 | Msa1387610 | 0.804908 | 1.723166e-49 | -8.615850e-47 |
| Msa0483000 | Msa1398110 | 0.813241 | 2.827495e-51 | -8.615850e-47 |
| Msa0483000 | Msa1398120 | 0.859180 | 4.941574e-63 | -8.615850e-47 |
| Msa0483000 | Msa1401110 | 0.812297 | 4.552095e-51 | -8.615850e-47 |
| Msa0483000 | Msa1412360 | 0.810725 | 9.993768e-51 | -8.615850e-47 |
| Msa0483000 | Msa1427000 | 0.812067 | 5.107451e-51 | -8.615850e-47 |
| Msa0483000 | Msa1429190 | 0.812783 | 3.563376e-51 | -8.615850e-47 |
| Msa0483000 | Msa1434850 | -0.804560 | 2.037276e-49 | -8.615850e-47 |
| Msa0483000 | Msa1438160 | 0.840884 | 6.644540e-58 | -8.615850e-47 |
| Msa0483000 | Msa1439780 | 0.809422 | 1.907607e-50 | -8.615850e-47 |
| Msa0483000 | Msa1449470 | 0.800679 | 1.287069e-48 | -8.615850e-47 |
| Msa0483000 | Msa1450970 | 0.968966 | 1.959981e-129 | -8.615850e-47 |
| Msa0483000 | Msa1453600 | 0.829745 | 4.333691e-55 | -8.615850e-47 |
| Msa0483000 | Msa1453890 | 0.807123 | 5.895021e-50 | -8.615850e-47 |
| Msa0483000 | Msa1459920 | 0.813880 | 2.045925e-51 | -8.615850e-47 |
| Msa0483000 | Msa1459930 | 0.851922 | 6.471079e-61 | -8.615850e-47 |
| Msa0483000 | Msa1464140 | 0.815317 | 9.835607e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483000 | MtrunA17_Chr4g0073821 | 100.000 | 77 | 0 | 0 | 1 | 77 | 1 | 77 | 7.81e-51 | 153 |
| Msa0483000 | MtrunA17_Chr4g0073811 | 81.333 | 75 | 14 | 0 | 1 | 75 | 1 | 75 | 1.22e-40 | 127 |
| Msa0483000 | MtrunA17_Chr7g0271271 | 70.833 | 48 | 13 | 1 | 8 | 55 | 6 | 52 | 2.43e-16 | 65.9 |
| Msa0483000 | MtrunA17_Chr7g0271281 | 62.500 | 48 | 17 | 1 | 8 | 55 | 6 | 52 | 2.29e-14 | 60.8 |
| Msa0483000 | MtrunA17_Chr6g0462381 | 55.769 | 52 | 22 | 1 | 5 | 56 | 3 | 53 | 1.91e-13 | 58.5 |
| Msa0483000 | MtrunA17_Chr7g0271261 | 61.702 | 47 | 17 | 1 | 9 | 55 | 7 | 52 | 1.58e-12 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0483000 | AT4G28088.1 | 80.263 | 76 | 14 | 1 | 1 | 75 | 1 | 76 | 8.21e-41 | 128 |
| Msa0483000 | AT2G24040.1 | 79.167 | 72 | 15 | 0 | 1 | 72 | 1 | 72 | 6.85e-38 | 121 |
| Msa0483000 | AT4G30660.1 | 75.342 | 73 | 18 | 0 | 1 | 73 | 1 | 73 | 4.70e-37 | 119 |
| Msa0483000 | AT4G30660.2 | 75.342 | 73 | 18 | 0 | 1 | 73 | 1 | 73 | 4.70e-37 | 119 |
| Msa0483000 | AT4G30650.1 | 63.514 | 74 | 26 | 1 | 1 | 74 | 1 | 73 | 2.63e-27 | 94.4 |
| Msa0483000 | AT2G38905.1 | 66.667 | 51 | 16 | 1 | 6 | 56 | 4 | 53 | 1.36e-15 | 64.3 |
| Msa0483000 | AT3G05880.1 | 69.565 | 46 | 13 | 1 | 10 | 55 | 8 | 52 | 1.71e-14 | 61.6 |
| Msa0483000 | AT3G05890.1 | 67.391 | 46 | 14 | 1 | 10 | 55 | 8 | 52 | 2.92e-14 | 60.8 |
| Msa0483000 | AT2G24040.2 | 75.000 | 32 | 8 | 0 | 1 | 32 | 1 | 32 | 4.71e-13 | 57.8 |
Find 30 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTGTGTTGCTCCCTCCTT+TGG | 0.237778 | 3_3:+90722324 | Msa0483000:CDS |
| AGATTTGAGTTAGGTCAATT+TGG | 0.259030 | 3_3:+90722820 | Msa0483000:three_prime_UTR |
| GATCATCACTTGTTCTTGTT+TGG | 0.268370 | 3_3:+90722227 | Msa0483000:five_prime_UTR |
| AATTCATGTAGAAAATTATA+TGG | 0.289444 | 3_3:+90722914 | Msa0483000:three_prime_UTR |
| ATTGTGGTTTACTTGAAAAT+AGG | 0.312089 | 3_3:+90722866 | Msa0483000:three_prime_UTR |
| TGATGTGATTGTTGATTGTT+TGG | 0.361552 | 3_3:+90722937 | Msa0483000:three_prime_UTR |
| ATCACGATCAATAAAGATAA+TGG | 0.374759 | 3_3:-90722675 | None:intergenic |
| GTGATGATCATCTAAAGTTT+AGG | 0.388258 | 3_3:-90722213 | None:intergenic |
| TGCTTGCTCCTTACAATACT+AGG | 0.414159 | 3_3:+90722620 | Msa0483000:CDS |
| CTTGTTCTTGTTTGGATCGC+GGG | 0.438467 | 3_3:+90722235 | Msa0483000:five_prime_UTR |
| TTCTCTTTGAGATTTGAGTT+AGG | 0.454642 | 3_3:+90722811 | Msa0483000:three_prime_UTR |
| TAAAGTGGTTAGTTATATTG+TGG | 0.465212 | 3_3:+90723002 | Msa0483000:three_prime_UTR |
| ACAATACTAGGATATCTTCC+TGG | 0.469622 | 3_3:+90722632 | Msa0483000:CDS |
| GCTGTGTTGCTCCCTCCTTT+GGG | 0.470326 | 3_3:+90722325 | Msa0483000:CDS |
| TTGAGTTAGGTCAATTTGGA+TGG | 0.478573 | 3_3:+90722824 | Msa0483000:three_prime_UTR |
| ATCAGTACTTTGATGAATAT+AGG | 0.494017 | 3_3:+90722696 | Msa0483000:CDS |
| ATCAAATTATGAAAATAAAG+TGG | 0.499775 | 3_3:+90722987 | Msa0483000:three_prime_UTR |
| GAAGATATCCTAGTATTGTA+AGG | 0.501846 | 3_3:-90722628 | None:intergenic |
| TTGCATTATTCGTTTCATTG+TGG | 0.556335 | 3_3:+90722850 | Msa0483000:three_prime_UTR |
| TTGGGTGTTTGTCTCCGCCA+TGG | 0.565764 | 3_3:+90722343 | Msa0483000:CDS |
| ACTTGTTCTTGTTTGGATCG+CGG | 0.583202 | 3_3:+90722234 | Msa0483000:five_prime_UTR |
| CAAGAATAATAAAATTCAAG+TGG | 0.594545 | 3_3:-90722778 | None:intergenic |
| GGAGACAAACACCCAAAGGA+GGG | 0.610468 | 3_3:-90722336 | None:intergenic |
| CGGAGACAAACACCCAAAGG+AGG | 0.614116 | 3_3:-90722337 | None:intergenic |
| TGGCGGAGACAAACACCCAA+AGG | 0.638625 | 3_3:-90722340 | None:intergenic |
| TAGAGAGCATAAATTATACC+AGG | 0.643088 | 3_3:-90722650 | None:intergenic |
| TATTGTGATTGTGCATACAA+AGG | 0.645143 | 3_3:-90722722 | None:intergenic |
| GACATACAGTGCAGCAACCA+TGG | 0.648406 | 3_3:-90722360 | None:intergenic |
| CAGCAAATTTCACAACGTGA+AGG | 0.664232 | 3_3:-90722289 | None:intergenic |
| ATACAGTGCAGCAACCATGG+CGG | 0.788306 | 3_3:-90722357 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAAAAATCATGAAAAAT+TGG | - | chr3_3:90722460-90722479 | None:intergenic | 15.0% |
| !! | AAATAATTTCATCAAAATCA+TGG | - | chr3_3:90722567-90722586 | None:intergenic | 15.0% |
| !!! | ATTTATATTTTAATTTGTGC+AGG | + | chr3_3:90722583-90722602 | Msa0483000:intron | 15.0% |
| !! | AATTCATGTAGAAAATTATA+TGG | + | chr3_3:90722914-90722933 | Msa0483000:three_prime_UTR | 15.0% |
| !! | ATCAAATTATGAAAATAAAG+TGG | + | chr3_3:90722987-90723006 | Msa0483000:three_prime_UTR | 15.0% |
| !! | ACTAAAAAATCATGAAAAAT+TGG | - | chr3_3:90722460-90722479 | None:intergenic | 15.0% |
| !! | AAATAATTTCATCAAAATCA+TGG | - | chr3_3:90722567-90722586 | None:intergenic | 15.0% |
| !!! | ATTTATATTTTAATTTGTGC+AGG | + | chr3_3:90722583-90722602 | Msa0483000:intron | 15.0% |
| !! | AATTCATGTAGAAAATTATA+TGG | + | chr3_3:90722914-90722933 | Msa0483000:three_prime_UTR | 15.0% |
| !! | ATCAAATTATGAAAATAAAG+TGG | + | chr3_3:90722987-90723006 | Msa0483000:three_prime_UTR | 15.0% |
| !! | AAAGGAAAAAAGAAGTAAAA+GGG | - | chr3_3:90722423-90722442 | None:intergenic | 20.0% |
| !! | AAAAGGAAAAAAGAAGTAAA+AGG | - | chr3_3:90722424-90722443 | None:intergenic | 20.0% |
| !!! | AATCATGAAAAATTGGATTT+TGG | - | chr3_3:90722453-90722472 | None:intergenic | 20.0% |
| !! | CAAGAATAATAAAATTCAAG+TGG | - | chr3_3:90722781-90722800 | None:intergenic | 20.0% |
| !! | AAAGGAAAAAAGAAGTAAAA+GGG | - | chr3_3:90722423-90722442 | None:intergenic | 20.0% |
| !! | AAAAGGAAAAAAGAAGTAAA+AGG | - | chr3_3:90722424-90722443 | None:intergenic | 20.0% |
| !!! | AATCATGAAAAATTGGATTT+TGG | - | chr3_3:90722453-90722472 | None:intergenic | 20.0% |
| !! | CAAGAATAATAAAATTCAAG+TGG | - | chr3_3:90722781-90722800 | None:intergenic | 20.0% |
| ! | ATCACGATCAATAAAGATAA+TGG | - | chr3_3:90722678-90722697 | None:intergenic | 25.0% |
| ! | ATCAGTACTTTGATGAATAT+AGG | + | chr3_3:90722696-90722715 | Msa0483000:CDS | 25.0% |
| ! | ATTGTGGTTTACTTGAAAAT+AGG | + | chr3_3:90722866-90722885 | Msa0483000:three_prime_UTR | 25.0% |
| !! | TAAAGTGGTTAGTTATATTG+TGG | + | chr3_3:90723002-90723021 | Msa0483000:three_prime_UTR | 25.0% |
| ! | ATCACGATCAATAAAGATAA+TGG | - | chr3_3:90722678-90722697 | None:intergenic | 25.0% |
| ! | ATCAGTACTTTGATGAATAT+AGG | + | chr3_3:90722696-90722715 | Msa0483000:CDS | 25.0% |
| ! | ATTGTGGTTTACTTGAAAAT+AGG | + | chr3_3:90722866-90722885 | Msa0483000:three_prime_UTR | 25.0% |
| ! | GTGATGATCATCTAAAGTTT+AGG | - | chr3_3:90722216-90722235 | None:intergenic | 30.0% |
| ! | TTGGATTTTGGATGTGAAAA+AGG | - | chr3_3:90722441-90722460 | None:intergenic | 30.0% |
| TTACAATAATTGTGAGTGAC+AGG | + | chr3_3:90722535-90722554 | Msa0483000:intron | 30.0% | |
| GAAGATATCCTAGTATTGTA+AGG | - | chr3_3:90722631-90722650 | None:intergenic | 30.0% | |
| ! | TAGAGAGCATAAATTATACC+AGG | - | chr3_3:90722653-90722672 | None:intergenic | 30.0% |
| TATTGTGATTGTGCATACAA+AGG | - | chr3_3:90722725-90722744 | None:intergenic | 30.0% | |
| !! | TTCTCTTTGAGATTTGAGTT+AGG | + | chr3_3:90722811-90722830 | Msa0483000:three_prime_UTR | 30.0% |
| ! | AGATTTGAGTTAGGTCAATT+TGG | + | chr3_3:90722820-90722839 | Msa0483000:three_prime_UTR | 30.0% |
| TTGCATTATTCGTTTCATTG+TGG | + | chr3_3:90722850-90722869 | Msa0483000:three_prime_UTR | 30.0% | |
| ! | TGATGTGATTGTTGATTGTT+TGG | + | chr3_3:90722937-90722956 | Msa0483000:three_prime_UTR | 30.0% |
| ! | GTGATGATCATCTAAAGTTT+AGG | - | chr3_3:90722216-90722235 | None:intergenic | 30.0% |
| ! | TTGGATTTTGGATGTGAAAA+AGG | - | chr3_3:90722441-90722460 | None:intergenic | 30.0% |
| TTACAATAATTGTGAGTGAC+AGG | + | chr3_3:90722535-90722554 | Msa0483000:intron | 30.0% | |
| GAAGATATCCTAGTATTGTA+AGG | - | chr3_3:90722631-90722650 | None:intergenic | 30.0% | |
| ! | TAGAGAGCATAAATTATACC+AGG | - | chr3_3:90722653-90722672 | None:intergenic | 30.0% |
| TATTGTGATTGTGCATACAA+AGG | - | chr3_3:90722725-90722744 | None:intergenic | 30.0% | |
| !! | TTCTCTTTGAGATTTGAGTT+AGG | + | chr3_3:90722811-90722830 | Msa0483000:three_prime_UTR | 30.0% |
| ! | AGATTTGAGTTAGGTCAATT+TGG | + | chr3_3:90722820-90722839 | Msa0483000:three_prime_UTR | 30.0% |
| TTGCATTATTCGTTTCATTG+TGG | + | chr3_3:90722850-90722869 | Msa0483000:three_prime_UTR | 30.0% | |
| ! | TGATGTGATTGTTGATTGTT+TGG | + | chr3_3:90722937-90722956 | Msa0483000:three_prime_UTR | 30.0% |
| GATCATCACTTGTTCTTGTT+TGG | + | chr3_3:90722227-90722246 | Msa0483000:five_prime_UTR | 35.0% | |
| !!! | CATTTTTTCTGGCTTCTTGT+TGG | - | chr3_3:90722269-90722288 | None:intergenic | 35.0% |
| ACAATACTAGGATATCTTCC+TGG | + | chr3_3:90722632-90722651 | Msa0483000:CDS | 35.0% | |
| TTGAGTTAGGTCAATTTGGA+TGG | + | chr3_3:90722824-90722843 | Msa0483000:three_prime_UTR | 35.0% | |
| GATCATCACTTGTTCTTGTT+TGG | + | chr3_3:90722227-90722246 | Msa0483000:five_prime_UTR | 35.0% | |
| !!! | CATTTTTTCTGGCTTCTTGT+TGG | - | chr3_3:90722269-90722288 | None:intergenic | 35.0% |
| ACAATACTAGGATATCTTCC+TGG | + | chr3_3:90722632-90722651 | Msa0483000:CDS | 35.0% | |
| TTGAGTTAGGTCAATTTGGA+TGG | + | chr3_3:90722824-90722843 | Msa0483000:three_prime_UTR | 35.0% | |
| ! | ACTTGTTCTTGTTTGGATCG+CGG | + | chr3_3:90722234-90722253 | Msa0483000:five_prime_UTR | 40.0% |
| !!! | CAACGTGAAGGCATTTTTTC+TGG | - | chr3_3:90722280-90722299 | None:intergenic | 40.0% |
| CAGCAAATTTCACAACGTGA+AGG | - | chr3_3:90722292-90722311 | None:intergenic | 40.0% | |
| TGCTTGCTCCTTACAATACT+AGG | + | chr3_3:90722620-90722639 | Msa0483000:CDS | 40.0% | |
| ! | ACTTGTTCTTGTTTGGATCG+CGG | + | chr3_3:90722234-90722253 | Msa0483000:five_prime_UTR | 40.0% |
| !!! | CAACGTGAAGGCATTTTTTC+TGG | - | chr3_3:90722280-90722299 | None:intergenic | 40.0% |
| CAGCAAATTTCACAACGTGA+AGG | - | chr3_3:90722292-90722311 | None:intergenic | 40.0% | |
| TGCTTGCTCCTTACAATACT+AGG | + | chr3_3:90722620-90722639 | Msa0483000:CDS | 40.0% | |
| ! | CTTGTTCTTGTTTGGATCGC+GGG | + | chr3_3:90722235-90722254 | Msa0483000:five_prime_UTR | 45.0% |
| TCTCTCTCCATCACTTCCAT+GGG | + | chr3_3:90722391-90722410 | Msa0483000:intron | 45.0% | |
| AGTAAAAGGGTAGATCCCCA+TGG | - | chr3_3:90722410-90722429 | None:intergenic | 45.0% | |
| ! | CTTGTTCTTGTTTGGATCGC+GGG | + | chr3_3:90722235-90722254 | Msa0483000:five_prime_UTR | 45.0% |
| TCTCTCTCCATCACTTCCAT+GGG | + | chr3_3:90722391-90722410 | Msa0483000:intron | 45.0% | |
| AGTAAAAGGGTAGATCCCCA+TGG | - | chr3_3:90722410-90722429 | None:intergenic | 45.0% | |
| GGAGACAAACACCCAAAGGA+GGG | - | chr3_3:90722339-90722358 | None:intergenic | 50.0% | |
| ATACAGTGCAGCAACCATGG+CGG | - | chr3_3:90722360-90722379 | None:intergenic | 50.0% | |
| GACATACAGTGCAGCAACCA+TGG | - | chr3_3:90722363-90722382 | None:intergenic | 50.0% | |
| CTCTCTCTCCATCACTTCCA+TGG | + | chr3_3:90722390-90722409 | Msa0483000:intron | 50.0% | |
| CTCTCTCCATCACTTCCATG+GGG | + | chr3_3:90722392-90722411 | Msa0483000:intron | 50.0% | |
| GTAGATCCCCATGGAAGTGA+TGG | - | chr3_3:90722401-90722420 | None:intergenic | 50.0% | |
| GGAGACAAACACCCAAAGGA+GGG | - | chr3_3:90722339-90722358 | None:intergenic | 50.0% | |
| ATACAGTGCAGCAACCATGG+CGG | - | chr3_3:90722360-90722379 | None:intergenic | 50.0% | |
| GACATACAGTGCAGCAACCA+TGG | - | chr3_3:90722363-90722382 | None:intergenic | 50.0% | |
| CTCTCTCTCCATCACTTCCA+TGG | + | chr3_3:90722390-90722409 | Msa0483000:intron | 50.0% | |
| CTCTCTCCATCACTTCCATG+GGG | + | chr3_3:90722392-90722411 | Msa0483000:intron | 50.0% | |
| GTAGATCCCCATGGAAGTGA+TGG | - | chr3_3:90722401-90722420 | None:intergenic | 50.0% | |
| ! | GCTGTGTTGCTCCCTCCTTT+GGG | + | chr3_3:90722325-90722344 | Msa0483000:CDS | 55.0% |
| CGGAGACAAACACCCAAAGG+AGG | - | chr3_3:90722340-90722359 | None:intergenic | 55.0% | |
| TGGCGGAGACAAACACCCAA+AGG | - | chr3_3:90722343-90722362 | None:intergenic | 55.0% | |
| TTGGGTGTTTGTCTCCGCCA+TGG | + | chr3_3:90722343-90722362 | Msa0483000:CDS | 55.0% | |
| ! | GCTGTGTTGCTCCCTCCTTT+GGG | + | chr3_3:90722325-90722344 | Msa0483000:CDS | 55.0% |
| CGGAGACAAACACCCAAAGG+AGG | - | chr3_3:90722340-90722359 | None:intergenic | 55.0% | |
| TGGCGGAGACAAACACCCAA+AGG | - | chr3_3:90722343-90722362 | None:intergenic | 55.0% | |
| TTGGGTGTTTGTCTCCGCCA+TGG | + | chr3_3:90722343-90722362 | Msa0483000:CDS | 55.0% | |
| ! | CGCTGTGTTGCTCCCTCCTT+TGG | + | chr3_3:90722324-90722343 | Msa0483000:CDS | 60.0% |
| ! | CGCTGTGTTGCTCCCTCCTT+TGG | + | chr3_3:90722324-90722343 | Msa0483000:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 90722181 | 90723024 | 90722181 | ID=Msa0483000;Name=Msa0483000 |
| chr3_3 | mRNA | 90722181 | 90723024 | 90722181 | ID=Msa0483000-mRNA-1;Parent=Msa0483000;Name=Msa0483000-mRNA-1;_AED=0.29;_eAED=0.29;_QI=105|1|1|1|1|1|2|276|77 |
| chr3_3 | exon | 90722181 | 90722375 | 90722181 | ID=Msa0483000-mRNA-1:exon:26464;Parent=Msa0483000-mRNA-1 |
| chr3_3 | exon | 90722605 | 90723024 | 90722605 | ID=Msa0483000-mRNA-1:exon:26465;Parent=Msa0483000-mRNA-1 |
| chr3_3 | five_prime_UTR | 90722181 | 90722285 | 90722181 | ID=Msa0483000-mRNA-1:five_prime_utr;Parent=Msa0483000-mRNA-1 |
| chr3_3 | CDS | 90722286 | 90722375 | 90722286 | ID=Msa0483000-mRNA-1:cds;Parent=Msa0483000-mRNA-1 |
| chr3_3 | CDS | 90722605 | 90722748 | 90722605 | ID=Msa0483000-mRNA-1:cds;Parent=Msa0483000-mRNA-1 |
| chr3_3 | three_prime_UTR | 90722749 | 90723024 | 90722749 | ID=Msa0483000-mRNA-1:three_prime_utr;Parent=Msa0483000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
