AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0646420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646420 MtrunA17_Chr4g0034761 92.708 96 5 1 1 96 11 104 2.09e-61 182
Msa0646420 MtrunA17_Chr5g0446661 60.215 93 30 2 1 87 1 92 1.01e-25 91.7
Msa0646420 MtrunA17_Chr3g0078771 61.538 78 24 1 1 72 1 78 2.44e-22 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646420 AT1G49310.1 42.254 71 37 2 8 74 12 82 7.49e-13 58.9

Find 29 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTCTTCTTCTGCTTCT+TGG 0.153872 4_3:-52192926 None:intergenic
TTAATATCTTATATGTTATA+TGG 0.245081 4_3:+52193016 Msa0646420:three_prime_UTR
GAAAAGACTTGGGAGATTAT+TGG 0.261061 4_3:+52192770 Msa0646420:CDS
AATTAGATTTGTTAGTTTGA+TGG 0.285742 4_3:-52192518 None:intergenic
CTCCCCAGATGTATTGTGTT+TGG 0.312718 4_3:+52193260 Msa0646420:three_prime_UTR
ACAAGTTCTTTGATTGCTTC+AGG 0.324229 4_3:-52192820 None:intergenic
TTGTTGGCACCAGTTGAAAT+TGG 0.331468 4_3:+52192426 None:intergenic
AAATACATCTATAGATAAAA+AGG 0.351346 4_3:-52193118 None:intergenic
AACTCTGAACAAGAGCTTCA+TGG 0.354626 4_3:+52192964 Msa0646420:CDS
AATACCAAACACAATACATC+TGG 0.373946 4_3:-52193264 None:intergenic
GATACATCAATCAAATCTAT+TGG 0.418852 4_3:-52193196 None:intergenic
CTCTTATGCAAGAAAAGACT+TGG 0.442343 4_3:+52192759 Msa0646420:CDS
GAGTATGAGAGAAGCAAAGA+TGG 0.451907 4_3:-52192575 None:intergenic
GATTTGTTAGTTTGATGGCT+AGG 0.457950 4_3:-52192513 None:intergenic
TCTTTGATTGCTTCAGGCAT+AGG 0.472850 4_3:-52192814 None:intergenic
ACGGTTGTACCAATTTCAAC+TGG 0.472939 4_3:-52192435 None:intergenic
AGTCAAAAGACAGTGAAACT+TGG 0.478801 4_3:+52193294 Msa0646420:three_prime_UTR
GTGTGATATAATATGACATT+AGG 0.480243 4_3:-52192892 None:intergenic
TGATTGCTTCAGGCATAGGT+TGG 0.514568 4_3:-52192810 None:intergenic
TGGTACAACCGTGTATAAAG+TGG 0.539054 4_3:+52192446 Msa0646420:five_prime_UTR
AGAACTTGTTCAAAATCAAG+AGG 0.539159 4_3:+52192834 Msa0646420:CDS
TTTGATTGATGTATCTAGTG+TGG 0.552142 4_3:+52193204 Msa0646420:three_prime_UTR
ATACCAAACACAATACATCT+GGG 0.600413 4_3:-52193263 None:intergenic
AAAAGACAGTGAAACTTGGA+TGG 0.605833 4_3:+52193298 Msa0646420:three_prime_UTR
TCTTATGCAAGAAAAGACTT+GGG 0.611202 4_3:+52192760 Msa0646420:CDS
TAAAAGTCCACATCTTAGCA+AGG 0.615141 4_3:+52192540 Msa0646420:five_prime_UTR
GGTACAACCGTGTATAAAGT+GGG 0.640534 4_3:+52192447 Msa0646420:five_prime_UTR
AAAGGGGCCCACTTTATACA+CGG 0.655601 4_3:-52192454 None:intergenic
TACCAAACACAATACATCTG+GGG 0.678240 4_3:-52193262 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATCTTATATGTTATA+TGG + chr4_3:52193016-52193035 Msa0646420:three_prime_UTR 10.0%
!! TTAATATCTTATATGTTATA+TGG + chr4_3:52193016-52193035 Msa0646420:three_prime_UTR 10.0%
!! ACAATATAGTATAAATTGAT+TGG - chr4_3:52192694-52192713 None:intergenic 15.0%
!!! AATTTTCTTTGTACTAAAAT+AGG + chr4_3:52192726-52192745 Msa0646420:intron 15.0%
!!! TATTTAAGGTTTATTTTGTA+GGG + chr4_3:52193051-52193070 Msa0646420:three_prime_UTR 15.0%
!! AAATACATCTATAGATAAAA+AGG - chr4_3:52193121-52193140 None:intergenic 15.0%
!!! TATCTATAGATGTATTTTTA+TGG + chr4_3:52193124-52193143 Msa0646420:three_prime_UTR 15.0%
!! ACAATATAGTATAAATTGAT+TGG - chr4_3:52192694-52192713 None:intergenic 15.0%
!!! AATTTTCTTTGTACTAAAAT+AGG + chr4_3:52192726-52192745 Msa0646420:intron 15.0%
!!! TATTTAAGGTTTATTTTGTA+GGG + chr4_3:52193051-52193070 Msa0646420:three_prime_UTR 15.0%
!! AAATACATCTATAGATAAAA+AGG - chr4_3:52193121-52193140 None:intergenic 15.0%
!!! TATCTATAGATGTATTTTTA+TGG + chr4_3:52193124-52193143 Msa0646420:three_prime_UTR 15.0%
!!! AATTAGATTTGTTAGTTTGA+TGG - chr4_3:52192521-52192540 None:intergenic 20.0%
!! TTAGTACAAAGAAAATTGAA+AGG - chr4_3:52192723-52192742 None:intergenic 20.0%
!! GGAAAAAAGATTAGTATTTA+AGG + chr4_3:52193037-52193056 Msa0646420:three_prime_UTR 20.0%
!!! GTATTTAAGGTTTATTTTGT+AGG + chr4_3:52193050-52193069 Msa0646420:three_prime_UTR 20.0%
!!! TAAAGTTGTATTTTTGTTGA+TGG + chr4_3:52193089-52193108 Msa0646420:three_prime_UTR 20.0%
!!! AATTAGATTTGTTAGTTTGA+TGG - chr4_3:52192521-52192540 None:intergenic 20.0%
!! TTAGTACAAAGAAAATTGAA+AGG - chr4_3:52192723-52192742 None:intergenic 20.0%
!! GGAAAAAAGATTAGTATTTA+AGG + chr4_3:52193037-52193056 Msa0646420:three_prime_UTR 20.0%
!!! GTATTTAAGGTTTATTTTGT+AGG + chr4_3:52193050-52193069 Msa0646420:three_prime_UTR 20.0%
!!! TAAAGTTGTATTTTTGTTGA+TGG + chr4_3:52193089-52193108 Msa0646420:three_prime_UTR 20.0%
!! TTCTTTTTATAGGCCAAAAA+GGG - chr4_3:52192474-52192493 None:intergenic 25.0%
!! TTTCTTTTTATAGGCCAAAA+AGG - chr4_3:52192475-52192494 None:intergenic 25.0%
!!! CTCTGACTATTTCTTTTTAT+AGG - chr4_3:52192484-52192503 None:intergenic 25.0%
! CTATAAAAAGAAATAGTCAG+AGG + chr4_3:52192482-52192501 Msa0646420:five_prime_UTR 25.0%
! GTGTGATATAATATGACATT+AGG - chr4_3:52192895-52192914 None:intergenic 25.0%
!!! TTTTTTGCTGCATCTATTTT+TGG + chr4_3:52193155-52193174 Msa0646420:three_prime_UTR 25.0%
!!! TTTTTGCTGCATCTATTTTT+GGG + chr4_3:52193156-52193175 Msa0646420:three_prime_UTR 25.0%
! GATACATCAATCAAATCTAT+TGG - chr4_3:52193199-52193218 None:intergenic 25.0%
!! TTCTTTTTATAGGCCAAAAA+GGG - chr4_3:52192474-52192493 None:intergenic 25.0%
!! TTTCTTTTTATAGGCCAAAA+AGG - chr4_3:52192475-52192494 None:intergenic 25.0%
!!! CTCTGACTATTTCTTTTTAT+AGG - chr4_3:52192484-52192503 None:intergenic 25.0%
! CTATAAAAAGAAATAGTCAG+AGG + chr4_3:52192482-52192501 Msa0646420:five_prime_UTR 25.0%
! GTGTGATATAATATGACATT+AGG - chr4_3:52192895-52192914 None:intergenic 25.0%
!!! TTTTTTGCTGCATCTATTTT+TGG + chr4_3:52193155-52193174 Msa0646420:three_prime_UTR 25.0%
!!! TTTTTGCTGCATCTATTTTT+GGG + chr4_3:52193156-52193175 Msa0646420:three_prime_UTR 25.0%
! GATACATCAATCAAATCTAT+TGG - chr4_3:52193199-52193218 None:intergenic 25.0%
! TCTTTTTATAGGCCAAAAAG+GGG - chr4_3:52192473-52192492 None:intergenic 30.0%
!!! ATTTTTTCCTTGCTAAGATG+TGG - chr4_3:52192550-52192569 None:intergenic 30.0%
TCTTATGCAAGAAAAGACTT+GGG + chr4_3:52192760-52192779 Msa0646420:CDS 30.0%
! AGAACTTGTTCAAAATCAAG+AGG + chr4_3:52192834-52192853 Msa0646420:CDS 30.0%
CTTCATGGAACATAAAAACA+TGG + chr4_3:52192979-52192998 Msa0646420:exon 30.0%
ATGGAACATAAAAACATGGT+TGG + chr4_3:52192983-52193002 Msa0646420:exon 30.0%
TTTGATTGATGTATCTAGTG+TGG + chr4_3:52193204-52193223 Msa0646420:three_prime_UTR 30.0%
ATACCAAACACAATACATCT+GGG - chr4_3:52193266-52193285 None:intergenic 30.0%
AATACCAAACACAATACATC+TGG - chr4_3:52193267-52193286 None:intergenic 30.0%
! TCTTTTTATAGGCCAAAAAG+GGG - chr4_3:52192473-52192492 None:intergenic 30.0%
!!! ATTTTTTCCTTGCTAAGATG+TGG - chr4_3:52192550-52192569 None:intergenic 30.0%
TCTTATGCAAGAAAAGACTT+GGG + chr4_3:52192760-52192779 Msa0646420:CDS 30.0%
! AGAACTTGTTCAAAATCAAG+AGG + chr4_3:52192834-52192853 Msa0646420:CDS 30.0%
CTTCATGGAACATAAAAACA+TGG + chr4_3:52192979-52192998 Msa0646420:exon 30.0%
ATGGAACATAAAAACATGGT+TGG + chr4_3:52192983-52193002 Msa0646420:exon 30.0%
TTTGATTGATGTATCTAGTG+TGG + chr4_3:52193204-52193223 Msa0646420:three_prime_UTR 30.0%
ATACCAAACACAATACATCT+GGG - chr4_3:52193266-52193285 None:intergenic 30.0%
AATACCAAACACAATACATC+TGG - chr4_3:52193267-52193286 None:intergenic 30.0%
!! GATTTGTTAGTTTGATGGCT+AGG - chr4_3:52192516-52192535 None:intergenic 35.0%
TAAAAGTCCACATCTTAGCA+AGG + chr4_3:52192540-52192559 Msa0646420:five_prime_UTR 35.0%
! CATACTCTTTTCTCTTCTTC+TGG + chr4_3:52192591-52192610 Msa0646420:CDS 35.0%
! AGTCTTTTCTTGCATAAGAG+AGG - chr4_3:52192759-52192778 None:intergenic 35.0%
CTCTTATGCAAGAAAAGACT+TGG + chr4_3:52192759-52192778 Msa0646420:CDS 35.0%
GAAAAGACTTGGGAGATTAT+TGG + chr4_3:52192770-52192789 Msa0646420:CDS 35.0%
ACAAGTTCTTTGATTGCTTC+AGG - chr4_3:52192823-52192842 None:intergenic 35.0%
! TTGTTTCTTCTTCTGCTTCT+TGG - chr4_3:52192929-52192948 None:intergenic 35.0%
TACCAAACACAATACATCTG+GGG - chr4_3:52193265-52193284 None:intergenic 35.0%
AGTCAAAAGACAGTGAAACT+TGG + chr4_3:52193294-52193313 Msa0646420:three_prime_UTR 35.0%
AAAAGACAGTGAAACTTGGA+TGG + chr4_3:52193298-52193317 Msa0646420:three_prime_UTR 35.0%
GAAACTTGGATGGTATTTGA+TGG + chr4_3:52193308-52193327 Msa0646420:three_prime_UTR 35.0%
!! GATTTGTTAGTTTGATGGCT+AGG - chr4_3:52192516-52192535 None:intergenic 35.0%
TAAAAGTCCACATCTTAGCA+AGG + chr4_3:52192540-52192559 Msa0646420:five_prime_UTR 35.0%
! CATACTCTTTTCTCTTCTTC+TGG + chr4_3:52192591-52192610 Msa0646420:CDS 35.0%
! AGTCTTTTCTTGCATAAGAG+AGG - chr4_3:52192759-52192778 None:intergenic 35.0%
CTCTTATGCAAGAAAAGACT+TGG + chr4_3:52192759-52192778 Msa0646420:CDS 35.0%
GAAAAGACTTGGGAGATTAT+TGG + chr4_3:52192770-52192789 Msa0646420:CDS 35.0%
ACAAGTTCTTTGATTGCTTC+AGG - chr4_3:52192823-52192842 None:intergenic 35.0%
! TTGTTTCTTCTTCTGCTTCT+TGG - chr4_3:52192929-52192948 None:intergenic 35.0%
TACCAAACACAATACATCTG+GGG - chr4_3:52193265-52193284 None:intergenic 35.0%
AGTCAAAAGACAGTGAAACT+TGG + chr4_3:52193294-52193313 Msa0646420:three_prime_UTR 35.0%
AAAAGACAGTGAAACTTGGA+TGG + chr4_3:52193298-52193317 Msa0646420:three_prime_UTR 35.0%
TGGTACAACCGTGTATAAAG+TGG + chr4_3:52192446-52192465 Msa0646420:five_prime_UTR 40.0%
GGTACAACCGTGTATAAAGT+GGG + chr4_3:52192447-52192466 Msa0646420:five_prime_UTR 40.0%
GAGTATGAGAGAAGCAAAGA+TGG - chr4_3:52192578-52192597 None:intergenic 40.0%
TCTTTGATTGCTTCAGGCAT+AGG - chr4_3:52192817-52192836 None:intergenic 40.0%
AACTCTGAACAAGAGCTTCA+TGG + chr4_3:52192964-52192983 Msa0646420:CDS 40.0%
TGGTACAACCGTGTATAAAG+TGG + chr4_3:52192446-52192465 Msa0646420:five_prime_UTR 40.0%
GGTACAACCGTGTATAAAGT+GGG + chr4_3:52192447-52192466 Msa0646420:five_prime_UTR 40.0%
GAGTATGAGAGAAGCAAAGA+TGG - chr4_3:52192578-52192597 None:intergenic 40.0%
TCTTTGATTGCTTCAGGCAT+AGG - chr4_3:52192817-52192836 None:intergenic 40.0%
AACTCTGAACAAGAGCTTCA+TGG + chr4_3:52192964-52192983 Msa0646420:CDS 40.0%
AAAGGGGCCCACTTTATACA+CGG - chr4_3:52192457-52192476 None:intergenic 45.0%
! GTATAAAGTGGGCCCCTTTT+TGG + chr4_3:52192458-52192477 Msa0646420:five_prime_UTR 45.0%
TGATTGCTTCAGGCATAGGT+TGG - chr4_3:52192813-52192832 None:intergenic 45.0%
CTCCCCAGATGTATTGTGTT+TGG + chr4_3:52193260-52193279 Msa0646420:three_prime_UTR 45.0%
AAAGGGGCCCACTTTATACA+CGG - chr4_3:52192457-52192476 None:intergenic 45.0%
! GTATAAAGTGGGCCCCTTTT+TGG + chr4_3:52192458-52192477 Msa0646420:five_prime_UTR 45.0%
TGATTGCTTCAGGCATAGGT+TGG - chr4_3:52192813-52192832 None:intergenic 45.0%
CTCCCCAGATGTATTGTGTT+TGG + chr4_3:52193260-52193279 Msa0646420:three_prime_UTR 45.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 52192438 52193329 52192438 ID=Msa0646420;Name=Msa0646420
chr4_3 mRNA 52192438 52193329 52192438 ID=Msa0646420-mRNA-1;Parent=Msa0646420;Name=Msa0646420-mRNA-1;_AED=0.08;_eAED=0.08;_QI=130|1|1|1|1|1|2|336|96
chr4_3 exon 52192438 52192612 52192438 ID=Msa0646420-mRNA-1:exon:12770;Parent=Msa0646420-mRNA-1
chr4_3 exon 52192748 52193329 52192748 ID=Msa0646420-mRNA-1:exon:12771;Parent=Msa0646420-mRNA-1
chr4_3 five_prime_UTR 52192438 52192567 52192438 ID=Msa0646420-mRNA-1:five_prime_utr;Parent=Msa0646420-mRNA-1
chr4_3 CDS 52192568 52192612 52192568 ID=Msa0646420-mRNA-1:cds;Parent=Msa0646420-mRNA-1
chr4_3 CDS 52192748 52192993 52192748 ID=Msa0646420-mRNA-1:cds;Parent=Msa0646420-mRNA-1
chr4_3 three_prime_UTR 52192994 52193329 52192994 ID=Msa0646420-mRNA-1:three_prime_utr;Parent=Msa0646420-mRNA-1
Gene Sequence

>Msa0646420

ATGAAGTCCATCTTTGCTTCTCTCATACTCTTTTCTCTTCTTCTGGTTGCTGACCTCTCTTATGCAAGAAAAGACTTGGGAGATTATTGGAAGAATATGATGAATGACCAACCTATGCCTGAAGCAATCAAAGAACTTGTTCAAAATCAAGAGGTTTCAGATGACTTTATTAGAGACTTTGATGTAAAGCCTAATGTCATATTATATCACACACATGCTGAATCCAAGAAGCAGAAGAAGAAACAACATGTCTTTGTGAAAAACTCTGAACAAGAGCTTCATGGAACATAA

Protein sequence

>Msa0646420

MKSIFASLILFSLLLVADLSYARKDLGDYWKNMMNDQPMPEAIKELVQNQEVSDDFIRDFDVKPNVILYHTHAESKKQKKKQHVFVKNSEQELHGT*