AlfalfaGEDB | Msa0220990

M. sativa cultivar zhongmu-4 / Msa0220990

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0012505 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0097708
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220990	XP_013465182.1	99.535	215	1	0	1	215	1	215	6.10e-155	438

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220990	sp\|Q40521\|RB11B_TOBAC	81.429	210	39	0	5	214	8	217	3.23e-127	360

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220990	G7ZUL4	99.535	215	1	0	1	215	1	215	2.91e-155	438

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0067590	Msa0220990	-0.804296	2.312116e-49	-8.615850e-47
Msa0105480	Msa0220990	-0.809370	1.957039e-50	-8.615850e-47
Msa0120880	Msa0220990	0.804837	1.783352e-49	-8.615850e-47
Msa0140770	Msa0220990	-0.832564	8.795820e-56	-8.615850e-47
Msa0179030	Msa0220990	0.820174	7.885573e-53	-8.615850e-47
Msa0202490	Msa0220990	0.801370	9.295569e-49	-8.615850e-47
Msa0209140	Msa0220990	0.818015	2.443948e-52	-8.615850e-47
Msa0217670	Msa0220990	0.802087	6.626471e-49	-8.615850e-47
Msa0220990	Msa0241830	0.813318	2.719516e-51	-8.615850e-47
Msa0220990	Msa0244970	0.802716	4.917114e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220990	MtrunA17_Chr2g0325091	99.535	215	1	0	1	215	1	215	5.61e-159	438
Msa0220990	MtrunA17_Chr4g0046261	81.019	216	38	2	1	213	1	216	1.10e-128	361
Msa0220990	MtrunA17_Chr2g0283131	80.556	216	39	2	1	213	1	216	1.79e-128	361
Msa0220990	MtrunA17_Chr4g0040781	81.019	216	38	2	1	213	1	216	2.83e-128	360
Msa0220990	MtrunA17_Chr2g0315551	76.415	212	47	3	5	214	7	217	5.20e-118	334
Msa0220990	MtrunA17_Chr2g0319951	72.170	212	55	2	5	213	7	217	7.18e-108	308
Msa0220990	MtrunA17_Chr3g0112681	67.453	212	66	2	4	213	5	215	2.83e-102	294
Msa0220990	MtrunA17_Chr2g0276941	66.038	212	69	1	5	213	9	220	1.77e-101	293
Msa0220990	MtrunA17_Chr4g0032201	67.136	213	66	3	4	214	5	215	6.00e-101	291
Msa0220990	MtrunA17_Chr1g0174281	58.065	217	85	2	2	212	6	222	2.28e-89	262

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220990	AT5G60860.1	81.429	210	38	1	5	213	7	216	8.61e-127	357
Msa0220990	AT3G15060.1	78.199	211	45	1	5	214	7	217	4.91e-123	347
Msa0220990	AT4G18430.1	76.852	216	47	2	1	213	1	216	3.38e-122	345
Msa0220990	AT1G28550.1	76.498	217	47	3	1	213	1	217	7.46e-122	344
Msa0220990	AT2G33870.1	76.415	212	48	2	5	214	7	218	1.02e-119	339
Msa0220990	AT5G45750.1	77.619	210	45	2	5	213	7	215	1.30e-118	336
Msa0220990	AT4G18800.1	77.143	210	44	2	5	213	7	213	5.30e-117	332
Msa0220990	AT1G16920.1	73.460	211	54	2	4	213	6	215	8.28e-113	322
Msa0220990	AT1G06400.1	73.333	210	54	2	5	213	7	215	8.47e-113	322
Msa0220990	AT1G09630.1	65.728	213	70	2	5	215	6	217	1.20e-103	298

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Find 51 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCGATGGTAAAATAA+AGG	0.371266	2_1:+69511102	None:intergenic
AAATGTTGAGAGGTGGTTGA+AGG	0.552257	2_1:-69511556	Msa0220990:CDS
AAGACTCTTGAGAAAGAGAA+TGG	0.519287	2_1:-69511321	Msa0220990:CDS
AAGGGAGAGACCATCAATAT+TGG	0.434305	2_1:-69511282	Msa0220990:CDS
AATTTGCTACTCGCAGTGTC+AGG	0.455604	2_1:-69512767	Msa0220990:CDS
ACACTTGCAAGCTGTGTCAA+CGG	0.600216	2_1:-69511469	Msa0220990:CDS
AGAGATGTATACATTAGAAG+AGG	0.513980	2_1:-69511196	Msa0220990:three_prime_UTR
AGAGTAAATTCTCATCATTT+TGG	0.200341	2_1:-69511159	Msa0220990:three_prime_UTR
AGCAAATTCAACACCGATGG+TGG	0.523464	2_1:+69512782	None:intergenic
AGCTTGTTAAGGCTCAGATT+TGG	0.356509	2_1:-69512734	Msa0220990:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATACGATTACTTGTTCAAGT+TGG	-	chr2_1:69511167-69511186	Msa0220990:three_prime_UTR	30.0%
CACTAGAAATGAATTCAACT+TGG	-	chr2_1:69511239-69511258	Msa0220990:CDS	30.0%
AATGGAGTGAAAATGCAAAA+GGG	-	chr2_1:69511550-69511569	Msa0220990:CDS	30.0%
GGAAGAAATAAACAATGGTT+AGG	+	chr2_1:69511850-69511869	None:intergenic	30.0%
AGGAAAAAGTAAGTGCATAT+TGG	+	chr2_1:69512152-69512171	None:intergenic	30.0%
AATAAGAGACTACAAGATTC+AGG	+	chr2_1:69512375-69512394	None:intergenic	30.0%
TGACATTTGAAAATGTTGAG+AGG	-	chr2_1:69512487-69512506	Msa0220990:intron	30.0%
TACAAGCATGATTACAACAT+AGG	+	chr2_1:69512544-69512563	None:intergenic	30.0%
TATGTTGTAATCATGCTTGT+AGG	-	chr2_1:69512543-69512562	Msa0220990:intron	30.0%
AGAGATGTATACATTAGAAG+AGG	-	chr2_1:69512857-69512876	Msa0220990:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	69510995	69513080	69510995	ID=Msa0220990;Name=Msa0220990
chr2_1	mRNA	69510995	69513080	69510995	ID=Msa0220990-mRNA-1;Parent=Msa0220990;Name=Msa0220990-mRNA-1;_AED=0.02;_eAED=0.02;_QI=152\|1\|1\|1\|1\|1\|2\|235\|215
chr2_1	exon	69510995	69511662	69510995	ID=Msa0220990-mRNA-1:exon:19695;Parent=Msa0220990-mRNA-1
chr2_1	exon	69512714	69513080	69512714	ID=Msa0220990-mRNA-1:exon:19694;Parent=Msa0220990-mRNA-1
chr2_1	five_prime_UTR	69512929	69513080	69512929	ID=Msa0220990-mRNA-1:five_prime_utr;Parent=Msa0220990-mRNA-1
chr2_1	CDS	69512714	69512928	69512714	ID=Msa0220990-mRNA-1:cds;Parent=Msa0220990-mRNA-1
chr2_1	CDS	69511230	69511662	69511230	ID=Msa0220990-mRNA-1:cds;Parent=Msa0220990-mRNA-1
chr2_1	three_prime_UTR	69510995	69511229	69510995	ID=Msa0220990-mRNA-1:three_prime_utr;Parent=Msa0220990-mRNA-1

Gene Sequence

>Msa0220990

ATGGGTGCTGTAGACGAAGAATACGATTACTTGTTCAAGTTGGTTTTGATCGGTGATTCTGGTGTTGGAAAGTCCAATCTTTTATCTAGATTCACTAGAAATGAATTCAACTTGGAATCCAAATCCACCATCGGTGTTGAATTTGCTACTCGCAGTGTCAGGATTCTTGATAAGCTTGTTAAGGCTCAGATTTGGGATACTGCTGGTCAAGAAAGATATCGTGCAATCACAAGTGCATACTACCGTGGAGCTGTTGGCGCATTGCTGGTGTACGACTCAACAAGGCATGTGACATTTGAAAATGTTGAGAGGTGGTTGAAGGAGCTTCGAGATCATACAGATGCCTATGTTGTAATCATGCTTGTAGGAAACAAAGCAGATCTTCGACACTTGCAAGCTGTGTCAACGGAAGAAGCCACCACATTTGCTGAGAAAGAAAACATATATTTCATGGAAACTTCTGCACTAGAGTCACTGAATGTGGACAATGCTTTCGTGGAAGTGCTCACTCAAATATACAATGTTGTGAGTAAGAAGACTCTTGAGAAAGAGAATGGCTCTGCGTCAGTGCCTAAGGGAGAGACCATCAATATTGGTAAAGATGATGTTTCAGATGTTAAGAAGAGCGGATGCTGCAGTACAGCATAG

Protein sequence

>Msa0220990

MGAVDEEYDYLFKLVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSVRILDKLVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDSTRHVTFENVERWLKELRDHTDAYVVIMLVGNKADLRHLQAVSTEEATTFAEKENIYFMETSALESLNVDNAFVEVLTQIYNVVSKKTLEKENGSASVPKGETINIGKDDVSDVKKSGCCSTA*