AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0549950


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549950 MtrunA17_Chr4g0034761 92.708 96 5 1 1 96 11 104 2.09e-61 182
Msa0549950 MtrunA17_Chr5g0446661 60.215 93 30 2 1 87 1 92 1.01e-25 91.7
Msa0549950 MtrunA17_Chr3g0078771 61.538 78 24 1 1 72 1 78 2.44e-22 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549950 AT1G49310.1 42.254 71 37 2 8 74 12 82 7.49e-13 58.9

Find 29 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTCTTCTTCTGCTTCT+TGG 0.153872 4_1:-45279786 None:intergenic
TTAATATCTTATATGTTATA+TGG 0.245081 4_1:+45279876 Msa0549950:three_prime_UTR
GAAAAGACTTGGGAGATTAT+TGG 0.261061 4_1:+45279630 Msa0549950:CDS
AATTAGATTTGTTAGTTTGA+TGG 0.285742 4_1:-45279378 None:intergenic
CTCCCCAGATGTATTGTGTT+TGG 0.312718 4_1:+45280120 Msa0549950:three_prime_UTR
ACAAGTTCTTTGATTGCTTC+AGG 0.324229 4_1:-45279680 None:intergenic
TTGTTGGCACCAGTTGAAAT+TGG 0.331468 4_1:+45279286 None:intergenic
AAATACATCTATAGATAAAA+AGG 0.351346 4_1:-45279978 None:intergenic
AACTCTGAACAAGAGCTTCA+TGG 0.354626 4_1:+45279824 Msa0549950:CDS
AATACCAAACACAATACATC+TGG 0.373946 4_1:-45280124 None:intergenic
GATACATCAATCAAATCTAT+TGG 0.418852 4_1:-45280056 None:intergenic
CTCTTATGCAAGAAAAGACT+TGG 0.442343 4_1:+45279619 Msa0549950:CDS
GAGTATGAGAGAAGCAAAGA+TGG 0.451907 4_1:-45279435 None:intergenic
GATTTGTTAGTTTGATGGCT+AGG 0.457950 4_1:-45279373 None:intergenic
TCTTTGATTGCTTCAGGCAT+AGG 0.472850 4_1:-45279674 None:intergenic
ACGGTTGTACCAATTTCAAC+TGG 0.472939 4_1:-45279295 None:intergenic
AGTCAAAAGACAGTGAAACT+TGG 0.478801 4_1:+45280154 Msa0549950:three_prime_UTR
GTGTGATATAATATGACATT+AGG 0.480243 4_1:-45279752 None:intergenic
TGATTGCTTCAGGCATAGGT+TGG 0.514568 4_1:-45279670 None:intergenic
TGGTACAACCGTGTATAAAG+TGG 0.539054 4_1:+45279306 Msa0549950:five_prime_UTR
AGAACTTGTTCAAAATCAAG+AGG 0.539159 4_1:+45279694 Msa0549950:CDS
TTTGATTGATGTATCTAGTG+TGG 0.552142 4_1:+45280064 Msa0549950:three_prime_UTR
ATACCAAACACAATACATCT+GGG 0.600413 4_1:-45280123 None:intergenic
AAAAGACAGTGAAACTTGGA+TGG 0.605833 4_1:+45280158 Msa0549950:three_prime_UTR
TCTTATGCAAGAAAAGACTT+GGG 0.611202 4_1:+45279620 Msa0549950:CDS
TAAAAGTCCACATCTTAGCA+AGG 0.615141 4_1:+45279400 Msa0549950:five_prime_UTR
GGTACAACCGTGTATAAAGT+GGG 0.640534 4_1:+45279307 Msa0549950:five_prime_UTR
AAAGGGGCCCACTTTATACA+CGG 0.655601 4_1:-45279314 None:intergenic
TACCAAACACAATACATCTG+GGG 0.678240 4_1:-45280122 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATCTTATATGTTATA+TGG + chr4_1:45279876-45279895 Msa0549950:three_prime_UTR 10.0%
!! TTAATATCTTATATGTTATA+TGG + chr4_1:45279876-45279895 Msa0549950:three_prime_UTR 10.0%
!! ACAATATAGTATAAATTGAT+TGG - chr4_1:45279554-45279573 None:intergenic 15.0%
!!! AATTTTCTTTGTACTAAAAT+AGG + chr4_1:45279586-45279605 Msa0549950:intron 15.0%
!!! TATTTAAGGTTTATTTTGTA+GGG + chr4_1:45279911-45279930 Msa0549950:three_prime_UTR 15.0%
!! AAATACATCTATAGATAAAA+AGG - chr4_1:45279981-45280000 None:intergenic 15.0%
!!! TATCTATAGATGTATTTTTA+TGG + chr4_1:45279984-45280003 Msa0549950:three_prime_UTR 15.0%
!! ACAATATAGTATAAATTGAT+TGG - chr4_1:45279554-45279573 None:intergenic 15.0%
!!! AATTTTCTTTGTACTAAAAT+AGG + chr4_1:45279586-45279605 Msa0549950:intron 15.0%
!!! TATTTAAGGTTTATTTTGTA+GGG + chr4_1:45279911-45279930 Msa0549950:three_prime_UTR 15.0%
!! AAATACATCTATAGATAAAA+AGG - chr4_1:45279981-45280000 None:intergenic 15.0%
!!! TATCTATAGATGTATTTTTA+TGG + chr4_1:45279984-45280003 Msa0549950:three_prime_UTR 15.0%
!!! AATTAGATTTGTTAGTTTGA+TGG - chr4_1:45279381-45279400 None:intergenic 20.0%
!! TTAGTACAAAGAAAATTGAA+AGG - chr4_1:45279583-45279602 None:intergenic 20.0%
!! GGAAAAAAGATTAGTATTTA+AGG + chr4_1:45279897-45279916 Msa0549950:three_prime_UTR 20.0%
!!! GTATTTAAGGTTTATTTTGT+AGG + chr4_1:45279910-45279929 Msa0549950:three_prime_UTR 20.0%
!!! TAAAGTTGTATTTTTGTTGA+TGG + chr4_1:45279949-45279968 Msa0549950:three_prime_UTR 20.0%
!!! AATTAGATTTGTTAGTTTGA+TGG - chr4_1:45279381-45279400 None:intergenic 20.0%
!! TTAGTACAAAGAAAATTGAA+AGG - chr4_1:45279583-45279602 None:intergenic 20.0%
!! GGAAAAAAGATTAGTATTTA+AGG + chr4_1:45279897-45279916 Msa0549950:three_prime_UTR 20.0%
!!! GTATTTAAGGTTTATTTTGT+AGG + chr4_1:45279910-45279929 Msa0549950:three_prime_UTR 20.0%
!!! TAAAGTTGTATTTTTGTTGA+TGG + chr4_1:45279949-45279968 Msa0549950:three_prime_UTR 20.0%
!! TTCTTTTTATAGGCCAAAAA+GGG - chr4_1:45279334-45279353 None:intergenic 25.0%
!! TTTCTTTTTATAGGCCAAAA+AGG - chr4_1:45279335-45279354 None:intergenic 25.0%
!!! CTCTGACTATTTCTTTTTAT+AGG - chr4_1:45279344-45279363 None:intergenic 25.0%
! CTATAAAAAGAAATAGTCAG+AGG + chr4_1:45279342-45279361 Msa0549950:five_prime_UTR 25.0%
! GTGTGATATAATATGACATT+AGG - chr4_1:45279755-45279774 None:intergenic 25.0%
!!! TTTTTTGCTGCATCTATTTT+TGG + chr4_1:45280015-45280034 Msa0549950:three_prime_UTR 25.0%
!!! TTTTTGCTGCATCTATTTTT+GGG + chr4_1:45280016-45280035 Msa0549950:three_prime_UTR 25.0%
! GATACATCAATCAAATCTAT+TGG - chr4_1:45280059-45280078 None:intergenic 25.0%
!! TTCTTTTTATAGGCCAAAAA+GGG - chr4_1:45279334-45279353 None:intergenic 25.0%
!! TTTCTTTTTATAGGCCAAAA+AGG - chr4_1:45279335-45279354 None:intergenic 25.0%
!!! CTCTGACTATTTCTTTTTAT+AGG - chr4_1:45279344-45279363 None:intergenic 25.0%
! CTATAAAAAGAAATAGTCAG+AGG + chr4_1:45279342-45279361 Msa0549950:five_prime_UTR 25.0%
! GTGTGATATAATATGACATT+AGG - chr4_1:45279755-45279774 None:intergenic 25.0%
!!! TTTTTTGCTGCATCTATTTT+TGG + chr4_1:45280015-45280034 Msa0549950:three_prime_UTR 25.0%
!!! TTTTTGCTGCATCTATTTTT+GGG + chr4_1:45280016-45280035 Msa0549950:three_prime_UTR 25.0%
! GATACATCAATCAAATCTAT+TGG - chr4_1:45280059-45280078 None:intergenic 25.0%
! TCTTTTTATAGGCCAAAAAG+GGG - chr4_1:45279333-45279352 None:intergenic 30.0%
!!! ATTTTTTCCTTGCTAAGATG+TGG - chr4_1:45279410-45279429 None:intergenic 30.0%
TCTTATGCAAGAAAAGACTT+GGG + chr4_1:45279620-45279639 Msa0549950:CDS 30.0%
! AGAACTTGTTCAAAATCAAG+AGG + chr4_1:45279694-45279713 Msa0549950:CDS 30.0%
CTTCATGGAACATAAAAACA+TGG + chr4_1:45279839-45279858 Msa0549950:exon 30.0%
ATGGAACATAAAAACATGGT+TGG + chr4_1:45279843-45279862 Msa0549950:exon 30.0%
TTTGATTGATGTATCTAGTG+TGG + chr4_1:45280064-45280083 Msa0549950:three_prime_UTR 30.0%
ATACCAAACACAATACATCT+GGG - chr4_1:45280126-45280145 None:intergenic 30.0%
AATACCAAACACAATACATC+TGG - chr4_1:45280127-45280146 None:intergenic 30.0%
! TCTTTTTATAGGCCAAAAAG+GGG - chr4_1:45279333-45279352 None:intergenic 30.0%
!!! ATTTTTTCCTTGCTAAGATG+TGG - chr4_1:45279410-45279429 None:intergenic 30.0%
TCTTATGCAAGAAAAGACTT+GGG + chr4_1:45279620-45279639 Msa0549950:CDS 30.0%
! AGAACTTGTTCAAAATCAAG+AGG + chr4_1:45279694-45279713 Msa0549950:CDS 30.0%
CTTCATGGAACATAAAAACA+TGG + chr4_1:45279839-45279858 Msa0549950:exon 30.0%
ATGGAACATAAAAACATGGT+TGG + chr4_1:45279843-45279862 Msa0549950:exon 30.0%
TTTGATTGATGTATCTAGTG+TGG + chr4_1:45280064-45280083 Msa0549950:three_prime_UTR 30.0%
ATACCAAACACAATACATCT+GGG - chr4_1:45280126-45280145 None:intergenic 30.0%
AATACCAAACACAATACATC+TGG - chr4_1:45280127-45280146 None:intergenic 30.0%
!! GATTTGTTAGTTTGATGGCT+AGG - chr4_1:45279376-45279395 None:intergenic 35.0%
TAAAAGTCCACATCTTAGCA+AGG + chr4_1:45279400-45279419 Msa0549950:five_prime_UTR 35.0%
! CATACTCTTTTCTCTTCTTC+TGG + chr4_1:45279451-45279470 Msa0549950:CDS 35.0%
! AGTCTTTTCTTGCATAAGAG+AGG - chr4_1:45279619-45279638 None:intergenic 35.0%
CTCTTATGCAAGAAAAGACT+TGG + chr4_1:45279619-45279638 Msa0549950:CDS 35.0%
GAAAAGACTTGGGAGATTAT+TGG + chr4_1:45279630-45279649 Msa0549950:CDS 35.0%
ACAAGTTCTTTGATTGCTTC+AGG - chr4_1:45279683-45279702 None:intergenic 35.0%
! TTGTTTCTTCTTCTGCTTCT+TGG - chr4_1:45279789-45279808 None:intergenic 35.0%
TACCAAACACAATACATCTG+GGG - chr4_1:45280125-45280144 None:intergenic 35.0%
AGTCAAAAGACAGTGAAACT+TGG + chr4_1:45280154-45280173 Msa0549950:three_prime_UTR 35.0%
AAAAGACAGTGAAACTTGGA+TGG + chr4_1:45280158-45280177 Msa0549950:three_prime_UTR 35.0%
GAAACTTGGATGGTATTTGA+TGG + chr4_1:45280168-45280187 Msa0549950:three_prime_UTR 35.0%
!! GATTTGTTAGTTTGATGGCT+AGG - chr4_1:45279376-45279395 None:intergenic 35.0%
TAAAAGTCCACATCTTAGCA+AGG + chr4_1:45279400-45279419 Msa0549950:five_prime_UTR 35.0%
! CATACTCTTTTCTCTTCTTC+TGG + chr4_1:45279451-45279470 Msa0549950:CDS 35.0%
! AGTCTTTTCTTGCATAAGAG+AGG - chr4_1:45279619-45279638 None:intergenic 35.0%
CTCTTATGCAAGAAAAGACT+TGG + chr4_1:45279619-45279638 Msa0549950:CDS 35.0%
GAAAAGACTTGGGAGATTAT+TGG + chr4_1:45279630-45279649 Msa0549950:CDS 35.0%
ACAAGTTCTTTGATTGCTTC+AGG - chr4_1:45279683-45279702 None:intergenic 35.0%
! TTGTTTCTTCTTCTGCTTCT+TGG - chr4_1:45279789-45279808 None:intergenic 35.0%
TACCAAACACAATACATCTG+GGG - chr4_1:45280125-45280144 None:intergenic 35.0%
AGTCAAAAGACAGTGAAACT+TGG + chr4_1:45280154-45280173 Msa0549950:three_prime_UTR 35.0%
AAAAGACAGTGAAACTTGGA+TGG + chr4_1:45280158-45280177 Msa0549950:three_prime_UTR 35.0%
TGGTACAACCGTGTATAAAG+TGG + chr4_1:45279306-45279325 Msa0549950:five_prime_UTR 40.0%
GGTACAACCGTGTATAAAGT+GGG + chr4_1:45279307-45279326 Msa0549950:five_prime_UTR 40.0%
GAGTATGAGAGAAGCAAAGA+TGG - chr4_1:45279438-45279457 None:intergenic 40.0%
TCTTTGATTGCTTCAGGCAT+AGG - chr4_1:45279677-45279696 None:intergenic 40.0%
AACTCTGAACAAGAGCTTCA+TGG + chr4_1:45279824-45279843 Msa0549950:CDS 40.0%
TGGTACAACCGTGTATAAAG+TGG + chr4_1:45279306-45279325 Msa0549950:five_prime_UTR 40.0%
GGTACAACCGTGTATAAAGT+GGG + chr4_1:45279307-45279326 Msa0549950:five_prime_UTR 40.0%
GAGTATGAGAGAAGCAAAGA+TGG - chr4_1:45279438-45279457 None:intergenic 40.0%
TCTTTGATTGCTTCAGGCAT+AGG - chr4_1:45279677-45279696 None:intergenic 40.0%
AACTCTGAACAAGAGCTTCA+TGG + chr4_1:45279824-45279843 Msa0549950:CDS 40.0%
AAAGGGGCCCACTTTATACA+CGG - chr4_1:45279317-45279336 None:intergenic 45.0%
! GTATAAAGTGGGCCCCTTTT+TGG + chr4_1:45279318-45279337 Msa0549950:five_prime_UTR 45.0%
TGATTGCTTCAGGCATAGGT+TGG - chr4_1:45279673-45279692 None:intergenic 45.0%
CTCCCCAGATGTATTGTGTT+TGG + chr4_1:45280120-45280139 Msa0549950:three_prime_UTR 45.0%
AAAGGGGCCCACTTTATACA+CGG - chr4_1:45279317-45279336 None:intergenic 45.0%
! GTATAAAGTGGGCCCCTTTT+TGG + chr4_1:45279318-45279337 Msa0549950:five_prime_UTR 45.0%
TGATTGCTTCAGGCATAGGT+TGG - chr4_1:45279673-45279692 None:intergenic 45.0%
CTCCCCAGATGTATTGTGTT+TGG + chr4_1:45280120-45280139 Msa0549950:three_prime_UTR 45.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 45279298 45280189 45279298 ID=Msa0549950;Name=Msa0549950
chr4_1 mRNA 45279298 45280189 45279298 ID=Msa0549950-mRNA-1;Parent=Msa0549950;Name=Msa0549950-mRNA-1;_AED=0.08;_eAED=0.08;_QI=130|1|1|1|1|1|2|336|96
chr4_1 exon 45279298 45279472 45279298 ID=Msa0549950-mRNA-1:exon:11893;Parent=Msa0549950-mRNA-1
chr4_1 exon 45279608 45280189 45279608 ID=Msa0549950-mRNA-1:exon:11894;Parent=Msa0549950-mRNA-1
chr4_1 five_prime_UTR 45279298 45279427 45279298 ID=Msa0549950-mRNA-1:five_prime_utr;Parent=Msa0549950-mRNA-1
chr4_1 CDS 45279428 45279472 45279428 ID=Msa0549950-mRNA-1:cds;Parent=Msa0549950-mRNA-1
chr4_1 CDS 45279608 45279853 45279608 ID=Msa0549950-mRNA-1:cds;Parent=Msa0549950-mRNA-1
chr4_1 three_prime_UTR 45279854 45280189 45279854 ID=Msa0549950-mRNA-1:three_prime_utr;Parent=Msa0549950-mRNA-1
Gene Sequence

>Msa0549950

ATGAAGTCCATCTTTGCTTCTCTCATACTCTTTTCTCTTCTTCTGGTTGCTGACCTCTCTTATGCAAGAAAAGACTTGGGAGATTATTGGAAGAATATGATGAATGACCAACCTATGCCTGAAGCAATCAAAGAACTTGTTCAAAATCAAGAGGTTTCAGATGACTTTATTAGAGACTTTGATGTAAAGCCTAATGTCATATTATATCACACACATGCTGAATCCAAGAAGCAGAAGAAGAAACAACATGTCTTTGTGAAAAACTCTGAACAAGAGCTTCATGGAACATAA

Protein sequence

>Msa0549950

MKSIFASLILFSLLLVADLSYARKDLGDYWKNMMNDQPMPEAIKELVQNQEVSDDFIRDFDVKPNVILYHTHAESKKQKKKQHVFVKNSEQELHGT*