AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0404230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0404230 MtrunA17_Chr3g0132581 94.170 223 13 0 1 223 1 223 4.75e-151 418
Msa0404230 MtrunA17_Chr4g0008781 50.476 210 104 0 4 213 7 216 1.07e-72 220
Msa0404230 MtrunA17_Chr7g0240631 48.182 220 112 2 4 222 6 224 3.47e-71 216
Msa0404230 MtrunA17_Chr8g0379581 49.772 219 106 1 1 215 1 219 4.55e-71 216
Msa0404230 MtrunA17_Chr7g0240691 48.416 221 111 2 4 222 36 255 4.75e-71 217
Msa0404230 MtrunA17_Chr7g0240681 47.273 220 113 2 4 221 6 224 2.90e-70 214
Msa0404230 MtrunA17_Chr4g0008791 46.222 225 117 3 1 222 1 224 2.94e-70 214
Msa0404230 MtrunA17_Chr7g0240581 50.228 219 107 2 4 221 6 223 5.90e-70 213
Msa0404230 MtrunA17_Chr6g0483141 51.042 192 94 0 19 210 17 208 1.04e-69 213
Msa0404230 MtrunA17_Chr7g0240641 48.182 220 112 2 4 222 6 224 7.08e-69 210
Msa0404230 MtrunA17_Chr7g0240561 46.847 222 116 2 1 221 3 223 2.95e-68 209
Msa0404230 MtrunA17_Chr1g0196771 45.045 222 119 2 4 223 2 222 7.78e-68 207
Msa0404230 MtrunA17_Chr8g0379561 46.635 208 111 0 1 208 2 209 1.06e-66 205
Msa0404230 MtrunA17_Chr7g0240611 48.182 220 108 3 4 222 6 220 6.96e-66 202
Msa0404230 MtrunA17_Chr1g0212301 49.048 210 100 3 6 210 4 211 1.54e-65 202
Msa0404230 MtrunA17_Chr1g0196661 48.309 207 101 4 6 208 4 208 2.13e-65 201
Msa0404230 MtrunA17_Chr7g0240551 47.867 211 108 2 4 213 6 215 3.63e-65 201
Msa0404230 MtrunA17_Chr7g0240541 48.815 211 106 2 4 213 6 215 2.16e-64 199
Msa0404230 MtrunA17_Chr1g0196691 43.891 221 119 3 6 222 29 248 2.06e-63 197
Msa0404230 MtrunA17_Chr7g0240531 47.664 214 106 3 4 216 6 214 4.20e-62 193
Msa0404230 MtrunA17_Chr1g0196721 43.891 221 117 4 5 220 2 220 9.14e-62 192
Msa0404230 MtrunA17_Chr7g0240671 49.010 202 97 3 6 206 5 201 1.38e-61 192
Msa0404230 MtrunA17_Chr5g0417011 44.651 215 113 2 6 214 8 222 1.73e-61 191
Msa0404230 MtrunA17_Chr7g0240511 47.032 219 109 3 4 221 6 218 2.03e-61 191
Msa0404230 MtrunA17_Chr4g0029341 46.047 215 112 3 3 214 2 215 4.75e-61 190
Msa0404230 MtrunA17_Chr7g0240521 47.059 221 112 3 4 223 13 229 2.27e-60 189
Msa0404230 MtrunA17_Chr7g0240651 47.273 220 110 3 4 222 6 220 2.02e-57 181
Msa0404230 MtrunA17_Chr1g0196711 43.519 216 114 4 6 218 4 214 3.27e-57 181
Msa0404230 MtrunA17_Chr2g0312771 44.175 206 112 2 3 207 2 205 4.26e-56 177
Msa0404230 MtrunA17_Chr2g0312751 43.689 206 113 2 3 207 2 205 5.01e-56 177
Msa0404230 MtrunA17_Chr7g0240661 49.510 204 97 3 4 206 6 204 1.27e-55 177
Msa0404230 MtrunA17_Chr5g0415411 41.150 226 125 3 1 218 1 226 1.14e-52 169
Msa0404230 MtrunA17_Chr2g0312781 41.262 206 118 2 3 207 19 222 8.93e-52 167
Msa0404230 MtrunA17_Chr2g0312791 43.810 210 113 5 3 209 2 209 6.27e-51 164
Msa0404230 MtrunA17_Chr2g0312721 38.532 218 128 3 3 216 2 217 7.10e-51 164
Msa0404230 MtrunA17_Chr8g0363751 41.256 223 123 3 1 215 1 223 1.29e-50 164
Msa0404230 MtrunA17_Chr2g0312811 40.000 220 125 5 3 218 2 218 1.81e-50 163
Msa0404230 MtrunA17_Chr2g0312731 40.291 206 120 3 3 207 2 205 2.00e-49 160
Msa0404230 MtrunA17_Chr4g0069471 49.727 183 85 6 4 182 24 203 2.74e-46 152
Msa0404230 MtrunA17_Chr2g0312681 37.327 217 133 3 3 218 2 216 2.04e-45 150
Msa0404230 MtrunA17_Chr3g0107731 45.556 180 96 2 2 180 6 184 2.74e-45 150
Msa0404230 MtrunA17_Chr3g0107741 47.561 164 85 1 1 163 5 168 5.02e-45 149
Msa0404230 MtrunA17_Chr8g0357241 47.531 162 84 1 3 163 2 163 3.51e-44 147
Msa0404230 MtrunA17_Chr8g0357211 46.914 162 85 1 3 163 2 163 7.55e-44 146
Msa0404230 MtrunA17_Chr5g0433801 37.037 216 133 2 3 217 4 217 8.86e-41 139
Msa0404230 MtrunA17_Chr2g0312691 37.788 217 132 3 3 218 2 216 3.75e-40 137
Msa0404230 MtrunA17_Chr8g0391631 43.396 159 82 3 1 151 17 175 1.32e-38 131
Msa0404230 MtrunA17_Chr2g0312711 37.788 217 131 4 3 218 2 215 3.89e-37 129
Msa0404230 MtrunA17_Chr2g0313561 50.000 58 28 1 49 105 2 59 2.85e-17 73.6
Msa0404230 MtrunA17_Chr5g0415421 39.726 73 41 1 29 98 11 83 9.94e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0404230 AT2G29420.1 43.891 221 124 0 3 223 7 227 3.26e-67 206
Msa0404230 AT3G09270.1 46.818 220 115 2 4 221 5 224 3.38e-67 206
Msa0404230 AT2G29460.1 44.749 219 120 1 4 221 6 224 2.61e-63 196
Msa0404230 AT2G29480.1 45.238 210 114 1 4 212 6 215 5.31e-62 193
Msa0404230 AT2G29490.1 42.009 219 126 1 4 221 6 224 3.50e-60 189
Msa0404230 AT2G29470.1 43.541 209 116 2 4 210 6 214 7.50e-59 185
Msa0404230 AT2G29450.1 41.818 220 126 2 4 221 5 224 9.67e-58 182
Msa0404230 AT2G29440.1 40.000 220 131 1 3 221 4 223 1.30e-57 182
Msa0404230 AT1G10370.1 41.364 220 120 4 1 212 1 219 5.99e-53 170
Msa0404230 AT1G78370.1 43.333 210 116 3 8 216 7 214 1.17e-52 169
Msa0404230 AT1G78340.1 45.977 174 93 1 3 175 2 175 7.53e-52 167
Msa0404230 AT1G78320.1 40.553 217 124 4 4 218 3 216 1.76e-51 166
Msa0404230 AT5G62480.1 38.053 226 131 2 4 220 7 232 2.10e-50 164
Msa0404230 AT1G78380.1 40.465 215 125 3 3 216 2 214 7.61e-50 162
Msa0404230 AT1G17170.1 43.478 207 113 4 3 208 2 205 9.79e-50 162
Msa0404230 AT1G74590.1 38.767 227 126 4 6 223 8 230 1.24e-49 162
Msa0404230 AT1G59670.1 40.455 220 123 4 4 215 5 224 2.47e-48 159
Msa0404230 AT1G17190.1 39.631 217 128 3 1 216 1 215 4.85e-48 157
Msa0404230 AT1G59700.1 42.523 214 115 4 4 209 5 218 4.97e-48 158
Msa0404230 AT1G10360.1 36.530 219 132 3 1 212 1 219 1.09e-47 157
Msa0404230 AT1G17180.1 39.908 218 126 4 3 218 2 216 9.80e-47 154
Msa0404230 AT1G69930.1 40.376 213 121 3 6 212 13 225 2.44e-46 154
Msa0404230 AT3G43800.1 39.640 222 127 4 1 217 1 220 7.88e-45 149
Msa0404230 AT1G27130.1 39.450 218 124 4 3 212 4 221 9.99e-45 149
Msa0404230 AT1G53680.1 45.238 168 89 2 6 170 8 175 1.32e-42 144
Msa0404230 AT1G78360.1 47.500 160 81 3 6 162 5 164 1.87e-42 143
Msa0404230 AT1G69920.1 38.889 216 124 4 5 212 34 249 1.84e-40 139
Msa0404230 AT1G27140.1 37.037 216 127 4 3 209 4 219 9.53e-38 132
Msa0404230 AT1G78320.2 34.722 216 111 4 4 218 3 189 2.04e-35 124
Msa0404230 AT5G62480.2 31.111 225 122 2 4 220 7 206 1.02e-33 120
Msa0404230 AT5G62480.3 31.111 225 121 2 4 220 7 205 1.24e-33 120

Find 71 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCACGTTACTAGGGTTC+TGG 0.230426 3_2:-34999619 Msa0404230:CDS
AGAGGGCCAAGGCGCGATTT+TGG 0.235373 3_2:-34999361 Msa0404230:CDS
CTTGATATTATAGTATTCTA+TGG 0.251887 3_2:+34998335 Msa0404220:intron
TAAAATCTTCTTCAACATAT+TGG 0.276929 3_2:+34999540 Msa0404220:three_prime_UTR
TCGCGCCTTGGCCCTCTCAT+AGG 0.290203 3_2:+34999367 Msa0404220:three_prime_UTR
AAGTGGGCTCTTGAACTAAA+AGG 0.296086 3_2:-34999572 Msa0404230:CDS
ATTAACATATTGCATGATAT+TGG 0.298727 3_2:+34998532 Msa0404220:intron
TGATGTGTGTGTATGATATA+TGG 0.313334 3_2:-34998565 Msa0404230:three_prime_UTR
GGAGACTCACTCACGTTACT+AGG 0.328682 3_2:-34999626 Msa0404230:CDS
GAGCTCAGGTTCGTGTTTAT+AGG 0.355178 3_2:+34998464 Msa0404220:intron
GACATCTTCATCAGCTTAAG+AGG 0.360365 3_2:-34998647 Msa0404230:exon
GGCGTGCATTCTTTGATAAC+AGG 0.364374 3_2:+34998718 Msa0404220:three_prime_UTR
TGTGTTTACATGATTAGTTT+AGG 0.367219 3_2:+34998438 Msa0404220:intron
AATCACTAGTGATTCTGATA+TGG 0.369693 3_2:+34999442 Msa0404220:three_prime_UTR
ACAGGTACCTTCTTATAAAC+AGG 0.378107 3_2:+34999485 Msa0404220:three_prime_UTR
GGCTTTGCTGATATTGCTGT+TGG 0.390337 3_2:-34998850 Msa0404230:CDS
TTCCCTCAAGTCCACTTTCA+AGG 0.391350 3_2:+34998899 Msa0404220:three_prime_UTR
CACTTGACCCTCAGGACAAA+TGG 0.402811 3_2:+34999590 Msa0404220:three_prime_UTR
GGCTAAATTCGCCGATGATA+AGG 0.406052 3_2:-34999339 Msa0404230:intron
ATCCTTGAAAGTGGACTTGA+GGG 0.416245 3_2:-34998901 Msa0404230:CDS
CCATGGACTCTGTATCAATC+AGG 0.416951 3_2:+34998779 Msa0404220:three_prime_UTR
CTTGAGGGAAGGCGCTACTT+TGG 0.416974 3_2:-34998886 Msa0404230:CDS
TAGAGTATGTTGATGAGATC+TGG 0.424571 3_2:-34999418 Msa0404230:CDS
TTGATATTATAGTATTCTAT+GGG 0.439636 3_2:+34998336 Msa0404220:intron
TACTTTGGAGGTGAAAATAT+AGG 0.440103 3_2:-34998871 Msa0404230:CDS
ATAACATTTGTTAGAGAACT+AGG 0.446766 3_2:-34999690 Msa0404230:five_prime_UTR
TATAACATATTGTATTGTAT+AGG 0.463518 3_2:+34998614 Msa0404220:intron
TTACCGTCATGCACAAGAAC+AGG 0.465185 3_2:+34999467 Msa0404220:three_prime_UTR
CATAGAATACTATAATATCA+AGG 0.468017 3_2:-34998334 Msa0404230:three_prime_UTR
CGCGCCTTGGCCCTCTCATA+GGG 0.469964 3_2:+34999368 Msa0404220:three_prime_UTR
AGTGGGCTCTTGAACTAAAA+GGG 0.471204 3_2:-34999571 Msa0404230:CDS
ACTCACGTTACTAGGGTTCT+GGG 0.475091 3_2:-34999618 Msa0404230:CDS
GAGGGAAGGCGCTACTTTGG+AGG 0.487384 3_2:-34998883 Msa0404230:CDS
GATGTGTGTGTATGATATAT+GGG 0.488055 3_2:-34998564 Msa0404230:three_prime_UTR
GCTTTGCTGATATTGCTGTT+GGG 0.489214 3_2:-34998849 Msa0404230:CDS
CAAGAGCCCACTTGACCCTC+AGG 0.492284 3_2:+34999582 Msa0404220:three_prime_UTR
ATTAGTTTAGGTTTGAGCTC+AGG 0.493653 3_2:+34998450 Msa0404220:intron
TAACGTCTTGATTAAATAAG+CGG 0.496299 3_2:+34998364 Msa0404220:intron
GGGTAGTCCATTTGTCCTGA+GGG 0.524592 3_2:-34999597 Msa0404230:CDS
CCTGATTGATACAGAGTCCA+TGG 0.533258 3_2:-34998779 Msa0404230:CDS
AATTTGAAGATCCTTGAAAG+TGG 0.535582 3_2:-34998910 Msa0404230:CDS
GGGGTAGTCCATTTGTCCTG+AGG 0.544193 3_2:-34999598 Msa0404230:CDS
GTATAATCCTGTTTATAAGA+AGG 0.544339 3_2:-34999492 Msa0404230:CDS
TTTAGCCCAAAATCGCGCCT+TGG 0.545817 3_2:+34999355 Msa0404220:three_prime_UTR
AAGAAAGTCATCGAAGTAAG+TGG 0.546614 3_2:+34998747 Msa0404220:three_prime_UTR
CATTTGTCCTGAGGGTCAAG+TGG 0.551709 3_2:-34999589 Msa0404230:CDS
AACTAGGAATGAGAAAGAGC+AGG 0.568013 3_2:-34999674 Msa0404230:five_prime_UTR
ATTTGTCCTGAGGGTCAAGT+GGG 0.573803 3_2:-34999588 Msa0404230:CDS
GCCCTCTCATAGGGATCATG+TGG 0.575393 3_2:+34999377 Msa0404220:three_prime_UTR
TAGGGATCATGTGGAAGAAG+AGG 0.575719 3_2:+34999386 Msa0404220:three_prime_UTR
GAGACTCACTCACGTTACTA+GGG 0.583299 3_2:-34999625 Msa0404230:CDS
GATCCTTGAAAGTGGACTTG+AGG 0.587591 3_2:-34998902 Msa0404230:CDS
GTAAGTGGTAAGTTTAGCCA+TGG 0.594981 3_2:+34998762 Msa0404220:three_prime_UTR
AATAGGAGAGGAACAACAGA+AGG 0.601970 3_2:-34998953 Msa0404230:CDS
ATAGAATACTATAATATCAA+GGG 0.602332 3_2:-34998333 Msa0404230:three_prime_UTR
TCCACATGATCCCTATGAGA+GGG 0.621697 3_2:-34999378 Msa0404230:CDS
ATGAAGATGTCAAGACCTTG+TGG 0.622264 3_2:+34998659 Msa0404220:three_prime_UTR
CATATTGTATTGTATAGGTG+TGG 0.627030 3_2:+34998619 Msa0404220:intron
GTGGGCTCTTGAACTAAAAG+GGG 0.630143 3_2:-34999570 Msa0404230:CDS
TTCCACATGATCCCTATGAG+AGG 0.630417 3_2:-34999379 Msa0404230:CDS
TTGAAAGTGGACTTGAGGGA+AGG 0.636293 3_2:-34998897 Msa0404230:CDS
GCACAGCAAGGCCTTCCACA+AGG 0.644456 3_2:-34998674 Msa0404230:CDS
AGATGTCAAGACCTTGTGGA+AGG 0.645290 3_2:+34998663 Msa0404220:three_prime_UTR
CTCACGTTACTAGGGTTCTG+GGG 0.648274 3_2:-34999617 Msa0404230:CDS
TCAAGTAACTTATCATGAGA+CGG 0.652385 3_2:+34998697 Msa0404220:three_prime_UTR
TGATCCCTATGAGAGGGCCA+AGG 0.658261 3_2:-34999372 Msa0404230:CDS
TAAGTTACTTGAGCACAGCA+AGG 0.675812 3_2:-34998686 Msa0404230:CDS
TGATATTATAGTATTCTATG+GGG 0.676578 3_2:+34998337 Msa0404220:intron
GTACCTGTTCTTGTGCATGA+CGG 0.690851 3_2:-34999470 Msa0404230:CDS
CGAGTGATAGAAGAAACTGT+AGG 0.708991 3_2:-34998808 Msa0404230:CDS
TGTGTGTATGATAATATGCG+TGG 0.738199 3_2:-34998494 Msa0404230:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAACATATTGTATTGTAT+AGG + chr3_2:34999397-34999416 Msa0404220:three_prime_UTR 15.0%
!!! TCTTGTAAAAGTAATTTTAA+TGG - chr3_2:34999617-34999636 Msa0404230:CDS 15.0%
!!! CTTGTAAAAGTAATTTTAAT+GGG - chr3_2:34999618-34999637 Msa0404230:CDS 15.0%
!! TTGATATTATAGTATTCTAT+GGG + chr3_2:34999675-34999694 Msa0404220:three_prime_UTR 15.0%
!! ATAGAATACTATAATATCAA+GGG - chr3_2:34999675-34999694 Msa0404230:five_prime_UTR 15.0%
!! TATAACATATTGTATTGTAT+AGG + chr3_2:34999397-34999416 Msa0404220:three_prime_UTR 15.0%
!!! TCTTGTAAAAGTAATTTTAA+TGG - chr3_2:34999617-34999636 Msa0404230:CDS 15.0%
!!! CTTGTAAAAGTAATTTTAAT+GGG - chr3_2:34999618-34999637 Msa0404230:CDS 15.0%
!! TTGATATTATAGTATTCTAT+GGG + chr3_2:34999675-34999694 Msa0404220:three_prime_UTR 15.0%
!! ATAGAATACTATAATATCAA+GGG - chr3_2:34999675-34999694 Msa0404230:five_prime_UTR 15.0%
!! TGTTTGATTAAAAGCAAAAA+TGG - chr3_2:34998357-34998376 Msa0404230:three_prime_UTR 20.0%
!! TAAAATCTTCTTCAACATAT+TGG + chr3_2:34998471-34998490 Msa0404220:intron 20.0%
!!! AATATGTTGAAGAAGATTTT+AGG - chr3_2:34998470-34998489 Msa0404230:three_prime_UTR 20.0%
!! ATTAACATATTGCATGATAT+TGG + chr3_2:34999479-34999498 Msa0404220:three_prime_UTR 20.0%
!! TGATATTATAGTATTCTATG+GGG + chr3_2:34999674-34999693 Msa0404220:three_prime_UTR 20.0%
!! CTTGATATTATAGTATTCTA+TGG + chr3_2:34999676-34999695 Msa0404220:three_prime_UTR 20.0%
!! CATAGAATACTATAATATCA+AGG - chr3_2:34999674-34999693 Msa0404230:five_prime_UTR 20.0%
!! TGTTTGATTAAAAGCAAAAA+TGG - chr3_2:34998357-34998376 Msa0404230:three_prime_UTR 20.0%
!! TAAAATCTTCTTCAACATAT+TGG + chr3_2:34998471-34998490 Msa0404220:intron 20.0%
!!! AATATGTTGAAGAAGATTTT+AGG - chr3_2:34998470-34998489 Msa0404230:three_prime_UTR 20.0%
!! ATTAACATATTGCATGATAT+TGG + chr3_2:34999479-34999498 Msa0404220:three_prime_UTR 20.0%
!! TGATATTATAGTATTCTATG+GGG + chr3_2:34999674-34999693 Msa0404220:three_prime_UTR 20.0%
!! CTTGATATTATAGTATTCTA+TGG + chr3_2:34999676-34999695 Msa0404220:three_prime_UTR 20.0%
!! CATAGAATACTATAATATCA+AGG - chr3_2:34999674-34999693 Msa0404230:five_prime_UTR 20.0%
!! ATAACATTTGTTAGAGAACT+AGG - chr3_2:34998318-34998337 Msa0404230:three_prime_UTR 25.0%
! GTATAATCCTGTTTATAAGA+AGG - chr3_2:34998516-34998535 Msa0404230:three_prime_UTR 25.0%
!!! CTTTTGAGTAATGGTTTAAT+TGG + chr3_2:34998852-34998871 Msa0404220:three_prime_UTR 25.0%
!!! ATAGCTACCTTTTTCAAAAT+AGG - chr3_2:34999038-34999057 Msa0404230:intron 25.0%
!!! TGTGTTTACATGATTAGTTT+AGG + chr3_2:34999573-34999592 Msa0404220:three_prime_UTR 25.0%
! TAACGTCTTGATTAAATAAG+CGG + chr3_2:34999647-34999666 Msa0404220:three_prime_UTR 25.0%
! TAGTTCTCTAACAAATGTTA+TGG + chr3_2:34998319-34998338 Msa0404220:intron 25.0%
!! ATAACATTTGTTAGAGAACT+AGG - chr3_2:34998318-34998337 Msa0404230:three_prime_UTR 25.0%
! GTATAATCCTGTTTATAAGA+AGG - chr3_2:34998516-34998535 Msa0404230:three_prime_UTR 25.0%
!!! CTTTTGAGTAATGGTTTAAT+TGG + chr3_2:34998852-34998871 Msa0404220:three_prime_UTR 25.0%
!!! ATAGCTACCTTTTTCAAAAT+AGG - chr3_2:34999038-34999057 Msa0404230:intron 25.0%
!!! TGTGTTTACATGATTAGTTT+AGG + chr3_2:34999573-34999592 Msa0404220:three_prime_UTR 25.0%
! TAACGTCTTGATTAAATAAG+CGG + chr3_2:34999647-34999666 Msa0404220:three_prime_UTR 25.0%
TGATTAAAAGCAAAAATGGC+AGG - chr3_2:34998361-34998380 Msa0404230:three_prime_UTR 30.0%
AATCACTAGTGATTCTGATA+TGG + chr3_2:34998569-34998588 Msa0404220:intron 30.0%
! TGGCTTTTATACCTTATCAT+CGG + chr3_2:34998683-34998702 Msa0404220:three_prime_UTR 30.0%
! ATCATGTTCTTTAGAAGTCA+TGG + chr3_2:34998703-34998722 Msa0404220:three_prime_UTR 30.0%
!! ATGTTGAGACTTTTGAGTAA+TGG + chr3_2:34998861-34998880 Msa0404220:three_prime_UTR 30.0%
!!! AAGGTTGGTTTTTGTATTAG+AGG + chr3_2:34998992-34999011 Msa0404220:intron 30.0%
AAAAAGGTAGCTATAAATGC+TGG + chr3_2:34999032-34999051 Msa0404220:intron 30.0%
!!! TACCTTTTTCAAAATAGGAG+AGG - chr3_2:34999043-34999062 Msa0404230:intron 30.0%
!! TTCCTCTCCTATTTTGAAAA+AGG + chr3_2:34999048-34999067 Msa0404220:intron 30.0%
AATTTGAAGATCCTTGAAAG+TGG - chr3_2:34999098-34999117 Msa0404230:intron 30.0%
! TACTTTGGAGGTGAAAATAT+AGG - chr3_2:34999137-34999156 Msa0404230:intron 30.0%
TCAAGTAACTTATCATGAGA+CGG + chr3_2:34999314-34999333 Msa0404220:intron 30.0%
CATATTGTATTGTATAGGTG+TGG + chr3_2:34999392-34999411 Msa0404220:three_prime_UTR 30.0%
TGATGTGTGTGTATGATATA+TGG - chr3_2:34999443-34999462 Msa0404230:CDS 30.0%
GATGTGTGTGTATGATATAT+GGG - chr3_2:34999444-34999463 Msa0404230:CDS 30.0%
TGATTAAAAGCAAAAATGGC+AGG - chr3_2:34998361-34998380 Msa0404230:three_prime_UTR 30.0%
AATCACTAGTGATTCTGATA+TGG + chr3_2:34998569-34998588 Msa0404220:intron 30.0%
! TGGCTTTTATACCTTATCAT+CGG + chr3_2:34998683-34998702 Msa0404220:three_prime_UTR 30.0%
! ATCATGTTCTTTAGAAGTCA+TGG + chr3_2:34998703-34998722 Msa0404220:three_prime_UTR 30.0%
!! ATGTTGAGACTTTTGAGTAA+TGG + chr3_2:34998861-34998880 Msa0404220:three_prime_UTR 30.0%
!!! AAGGTTGGTTTTTGTATTAG+AGG + chr3_2:34998992-34999011 Msa0404220:intron 30.0%
AAAAAGGTAGCTATAAATGC+TGG + chr3_2:34999032-34999051 Msa0404220:intron 30.0%
!!! TACCTTTTTCAAAATAGGAG+AGG - chr3_2:34999043-34999062 Msa0404230:intron 30.0%
!! TTCCTCTCCTATTTTGAAAA+AGG + chr3_2:34999048-34999067 Msa0404220:intron 30.0%
AATTTGAAGATCCTTGAAAG+TGG - chr3_2:34999098-34999117 Msa0404230:intron 30.0%
! TACTTTGGAGGTGAAAATAT+AGG - chr3_2:34999137-34999156 Msa0404230:intron 30.0%
TCAAGTAACTTATCATGAGA+CGG + chr3_2:34999314-34999333 Msa0404220:intron 30.0%
CATATTGTATTGTATAGGTG+TGG + chr3_2:34999392-34999411 Msa0404220:three_prime_UTR 30.0%
TGATGTGTGTGTATGATATA+TGG - chr3_2:34999443-34999462 Msa0404230:CDS 30.0%
GATGTGTGTGTATGATATAT+GGG - chr3_2:34999444-34999463 Msa0404230:CDS 30.0%
ACAGGTACCTTCTTATAAAC+AGG + chr3_2:34998526-34998545 Msa0404220:intron 35.0%
! TAGAGTATGTTGATGAGATC+TGG - chr3_2:34998590-34998609 Msa0404230:three_prime_UTR 35.0%
GATCAAACCAAACATCAAGT+TGG + chr3_2:34998759-34998778 Msa0404220:three_prime_UTR 35.0%
!! TTGGTTTTTGTATTAGAGGC+TGG + chr3_2:34998988-34999007 Msa0404220:three_prime_UTR 35.0%
CACACTGAAATAGAAAAGGT+TGG + chr3_2:34999007-34999026 Msa0404220:intron 35.0%
!!! GATATTGCTGTTGGGTTTTT+TGG - chr3_2:34999167-34999186 Msa0404230:intron 35.0%
!!! TGTTGGGTTTTTTGGATTCT+GGG - chr3_2:34999175-34999194 Msa0404230:intron 35.0%
AAGAAAGTCATCGAAGTAAG+TGG + chr3_2:34999264-34999283 Msa0404220:intron 35.0%
TGTGTGTATGATAATATGCG+TGG - chr3_2:34999514-34999533 Msa0404230:CDS 35.0%
! ATTAGTTTAGGTTTGAGCTC+AGG + chr3_2:34999561-34999580 Msa0404220:three_prime_UTR 35.0%
ACAGGTACCTTCTTATAAAC+AGG + chr3_2:34998526-34998545 Msa0404220:intron 35.0%
! TAGAGTATGTTGATGAGATC+TGG - chr3_2:34998590-34998609 Msa0404230:three_prime_UTR 35.0%
GATCAAACCAAACATCAAGT+TGG + chr3_2:34998759-34998778 Msa0404220:three_prime_UTR 35.0%
!! TTGGTTTTTGTATTAGAGGC+TGG + chr3_2:34998988-34999007 Msa0404220:three_prime_UTR 35.0%
CACACTGAAATAGAAAAGGT+TGG + chr3_2:34999007-34999026 Msa0404220:intron 35.0%
!!! GATATTGCTGTTGGGTTTTT+TGG - chr3_2:34999167-34999186 Msa0404230:intron 35.0%
!!! TGTTGGGTTTTTTGGATTCT+GGG - chr3_2:34999175-34999194 Msa0404230:intron 35.0%
AAGAAAGTCATCGAAGTAAG+TGG + chr3_2:34999264-34999283 Msa0404220:intron 35.0%
TGTGTGTATGATAATATGCG+TGG - chr3_2:34999514-34999533 Msa0404230:CDS 35.0%
! ATTAGTTTAGGTTTGAGCTC+AGG + chr3_2:34999561-34999580 Msa0404220:three_prime_UTR 35.0%
AACTAGGAATGAGAAAGAGC+AGG - chr3_2:34998334-34998353 Msa0404230:three_prime_UTR 40.0%
! AAGTGGGCTCTTGAACTAAA+AGG - chr3_2:34998436-34998455 Msa0404230:three_prime_UTR 40.0%
! AGTGGGCTCTTGAACTAAAA+GGG - chr3_2:34998437-34998456 Msa0404230:three_prime_UTR 40.0%
AAGAACATGATCAAACCGTC+TGG - chr3_2:34998712-34998731 Msa0404230:CDS 40.0%
! ATCAAACCGTCTGGTTCAAA+TGG - chr3_2:34998721-34998740 Msa0404230:CDS 40.0%
GTTCAACCATTTGAACCAGA+CGG + chr3_2:34998730-34998749 Msa0404220:three_prime_UTR 40.0%
!! CCAAACATCAAGTTGGTTTC+TGG + chr3_2:34998752-34998771 Msa0404220:three_prime_UTR 40.0%
CCAGAAACCAACTTGATGTT+TGG - chr3_2:34998749-34998768 Msa0404230:CDS 40.0%
AGTCTCAACATTTCGATCTC+TGG - chr3_2:34998870-34998889 Msa0404230:CDS 40.0%
GGCACACACTGAAATAGAAA+AGG + chr3_2:34999011-34999030 Msa0404220:intron 40.0%
AATAGGAGAGGAACAACAGA+AGG - chr3_2:34999055-34999074 Msa0404230:intron 40.0%
! ATCCTTGAAAGTGGACTTGA+GGG - chr3_2:34999107-34999126 Msa0404230:intron 40.0%
! GCTTTGCTGATATTGCTGTT+GGG - chr3_2:34999159-34999178 Msa0404230:intron 40.0%
!!! CTGTTGGGTTTTTTGGATTC+TGG - chr3_2:34999174-34999193 Msa0404230:intron 40.0%
CGAGTGATAGAAGAAACTGT+AGG - chr3_2:34999200-34999219 Msa0404230:intron 40.0%
GTAAGTGGTAAGTTTAGCCA+TGG + chr3_2:34999249-34999268 Msa0404220:intron 40.0%
TAAGTTACTTGAGCACAGCA+AGG - chr3_2:34999322-34999341 Msa0404230:intron 40.0%
ATGAAGATGTCAAGACCTTG+TGG + chr3_2:34999352-34999371 Msa0404220:three_prime_UTR 40.0%
GACATCTTCATCAGCTTAAG+AGG - chr3_2:34999361-34999380 Msa0404230:CDS 40.0%
AACTAGGAATGAGAAAGAGC+AGG - chr3_2:34998334-34998353 Msa0404230:three_prime_UTR 40.0%
! AAGTGGGCTCTTGAACTAAA+AGG - chr3_2:34998436-34998455 Msa0404230:three_prime_UTR 40.0%
! AGTGGGCTCTTGAACTAAAA+GGG - chr3_2:34998437-34998456 Msa0404230:three_prime_UTR 40.0%
AAGAACATGATCAAACCGTC+TGG - chr3_2:34998712-34998731 Msa0404230:CDS 40.0%
! ATCAAACCGTCTGGTTCAAA+TGG - chr3_2:34998721-34998740 Msa0404230:CDS 40.0%
GTTCAACCATTTGAACCAGA+CGG + chr3_2:34998730-34998749 Msa0404220:three_prime_UTR 40.0%
!! CCAAACATCAAGTTGGTTTC+TGG + chr3_2:34998752-34998771 Msa0404220:three_prime_UTR 40.0%
CCAGAAACCAACTTGATGTT+TGG - chr3_2:34998749-34998768 Msa0404230:CDS 40.0%
AGTCTCAACATTTCGATCTC+TGG - chr3_2:34998870-34998889 Msa0404230:CDS 40.0%
GGCACACACTGAAATAGAAA+AGG + chr3_2:34999011-34999030 Msa0404220:intron 40.0%
AATAGGAGAGGAACAACAGA+AGG - chr3_2:34999055-34999074 Msa0404230:intron 40.0%
! ATCCTTGAAAGTGGACTTGA+GGG - chr3_2:34999107-34999126 Msa0404230:intron 40.0%
! GCTTTGCTGATATTGCTGTT+GGG - chr3_2:34999159-34999178 Msa0404230:intron 40.0%
!!! CTGTTGGGTTTTTTGGATTC+TGG - chr3_2:34999174-34999193 Msa0404230:intron 40.0%
CGAGTGATAGAAGAAACTGT+AGG - chr3_2:34999200-34999219 Msa0404230:intron 40.0%
GTAAGTGGTAAGTTTAGCCA+TGG + chr3_2:34999249-34999268 Msa0404220:intron 40.0%
TAAGTTACTTGAGCACAGCA+AGG - chr3_2:34999322-34999341 Msa0404230:intron 40.0%
ATGAAGATGTCAAGACCTTG+TGG + chr3_2:34999352-34999371 Msa0404220:three_prime_UTR 40.0%
GACATCTTCATCAGCTTAAG+AGG - chr3_2:34999361-34999380 Msa0404230:CDS 40.0%
GAGACTCACTCACGTTACTA+GGG - chr3_2:34998383-34998402 Msa0404230:three_prime_UTR 45.0%
ACTCACGTTACTAGGGTTCT+GGG - chr3_2:34998390-34998409 Msa0404230:three_prime_UTR 45.0%
ATTTGTCCTGAGGGTCAAGT+GGG - chr3_2:34998420-34998439 Msa0404230:three_prime_UTR 45.0%
GTGGGCTCTTGAACTAAAAG+GGG - chr3_2:34998438-34998457 Msa0404230:three_prime_UTR 45.0%
GTACCTGTTCTTGTGCATGA+CGG - chr3_2:34998538-34998557 Msa0404230:three_prime_UTR 45.0%
TTACCGTCATGCACAAGAAC+AGG + chr3_2:34998544-34998563 Msa0404220:intron 45.0%
TAGGGATCATGTGGAAGAAG+AGG + chr3_2:34998625-34998644 Msa0404220:intron 45.0%
TTCCACATGATCCCTATGAG+AGG - chr3_2:34998629-34998648 Msa0404230:three_prime_UTR 45.0%
TCCACATGATCCCTATGAGA+GGG - chr3_2:34998630-34998649 Msa0404230:three_prime_UTR 45.0%
GGCTAAATTCGCCGATGATA+AGG - chr3_2:34998669-34998688 Msa0404230:CDS 45.0%
! GATCCTTGAAAGTGGACTTG+AGG - chr3_2:34999106-34999125 Msa0404230:intron 45.0%
TTCCCTCAAGTCCACTTTCA+AGG + chr3_2:34999112-34999131 Msa0404220:intron 45.0%
! TTGAAAGTGGACTTGAGGGA+AGG - chr3_2:34999111-34999130 Msa0404230:intron 45.0%
GGCTTTGCTGATATTGCTGT+TGG - chr3_2:34999158-34999177 Msa0404230:intron 45.0%
CCATGGACTCTGTATCAATC+AGG + chr3_2:34999232-34999251 Msa0404220:intron 45.0%
CCTGATTGATACAGAGTCCA+TGG - chr3_2:34999229-34999248 Msa0404230:intron 45.0%
! GGCGTGCATTCTTTGATAAC+AGG + chr3_2:34999293-34999312 Msa0404220:intron 45.0%
AGATGTCAAGACCTTGTGGA+AGG + chr3_2:34999348-34999367 Msa0404220:three_prime_UTR 45.0%
GAGCTCAGGTTCGTGTTTAT+AGG + chr3_2:34999547-34999566 Msa0404220:three_prime_UTR 45.0%
GAGACTCACTCACGTTACTA+GGG - chr3_2:34998383-34998402 Msa0404230:three_prime_UTR 45.0%
ACTCACGTTACTAGGGTTCT+GGG - chr3_2:34998390-34998409 Msa0404230:three_prime_UTR 45.0%
ATTTGTCCTGAGGGTCAAGT+GGG - chr3_2:34998420-34998439 Msa0404230:three_prime_UTR 45.0%
GTGGGCTCTTGAACTAAAAG+GGG - chr3_2:34998438-34998457 Msa0404230:three_prime_UTR 45.0%
GTACCTGTTCTTGTGCATGA+CGG - chr3_2:34998538-34998557 Msa0404230:three_prime_UTR 45.0%
TTACCGTCATGCACAAGAAC+AGG + chr3_2:34998544-34998563 Msa0404220:intron 45.0%
TAGGGATCATGTGGAAGAAG+AGG + chr3_2:34998625-34998644 Msa0404220:intron 45.0%
TTCCACATGATCCCTATGAG+AGG - chr3_2:34998629-34998648 Msa0404230:three_prime_UTR 45.0%
TCCACATGATCCCTATGAGA+GGG - chr3_2:34998630-34998649 Msa0404230:three_prime_UTR 45.0%
GGCTAAATTCGCCGATGATA+AGG - chr3_2:34998669-34998688 Msa0404230:CDS 45.0%
! GATCCTTGAAAGTGGACTTG+AGG - chr3_2:34999106-34999125 Msa0404230:intron 45.0%
TTCCCTCAAGTCCACTTTCA+AGG + chr3_2:34999112-34999131 Msa0404220:intron 45.0%
! TTGAAAGTGGACTTGAGGGA+AGG - chr3_2:34999111-34999130 Msa0404230:intron 45.0%
GGCTTTGCTGATATTGCTGT+TGG - chr3_2:34999158-34999177 Msa0404230:intron 45.0%
CCATGGACTCTGTATCAATC+AGG + chr3_2:34999232-34999251 Msa0404220:intron 45.0%
CCTGATTGATACAGAGTCCA+TGG - chr3_2:34999229-34999248 Msa0404230:intron 45.0%
! GGCGTGCATTCTTTGATAAC+AGG + chr3_2:34999293-34999312 Msa0404220:intron 45.0%
AGATGTCAAGACCTTGTGGA+AGG + chr3_2:34999348-34999367 Msa0404220:three_prime_UTR 45.0%
GAGCTCAGGTTCGTGTTTAT+AGG + chr3_2:34999547-34999566 Msa0404220:three_prime_UTR 45.0%
GGAGACTCACTCACGTTACT+AGG - chr3_2:34998382-34998401 Msa0404230:three_prime_UTR 50.0%
CACTCACGTTACTAGGGTTC+TGG - chr3_2:34998389-34998408 Msa0404230:three_prime_UTR 50.0%
CTCACGTTACTAGGGTTCTG+GGG - chr3_2:34998391-34998410 Msa0404230:three_prime_UTR 50.0%
GGGTAGTCCATTTGTCCTGA+GGG - chr3_2:34998411-34998430 Msa0404230:three_prime_UTR 50.0%
CACTTGACCCTCAGGACAAA+TGG + chr3_2:34998421-34998440 Msa0404220:intron 50.0%
CATTTGTCCTGAGGGTCAAG+TGG - chr3_2:34998419-34998438 Msa0404230:three_prime_UTR 50.0%
TTTAGCCCAAAATCGCGCCT+TGG + chr3_2:34998656-34998675 Msa0404220:three_prime_UTR 50.0%
GGAGACTCACTCACGTTACT+AGG - chr3_2:34998382-34998401 Msa0404230:three_prime_UTR 50.0%
CACTCACGTTACTAGGGTTC+TGG - chr3_2:34998389-34998408 Msa0404230:three_prime_UTR 50.0%
CTCACGTTACTAGGGTTCTG+GGG - chr3_2:34998391-34998410 Msa0404230:three_prime_UTR 50.0%
GGGTAGTCCATTTGTCCTGA+GGG - chr3_2:34998411-34998430 Msa0404230:three_prime_UTR 50.0%
CACTTGACCCTCAGGACAAA+TGG + chr3_2:34998421-34998440 Msa0404220:intron 50.0%
CATTTGTCCTGAGGGTCAAG+TGG - chr3_2:34998419-34998438 Msa0404230:three_prime_UTR 50.0%
TTTAGCCCAAAATCGCGCCT+TGG + chr3_2:34998656-34998675 Msa0404220:three_prime_UTR 50.0%
GGGGTAGTCCATTTGTCCTG+AGG - chr3_2:34998410-34998429 Msa0404230:three_prime_UTR 55.0%
GCCCTCTCATAGGGATCATG+TGG + chr3_2:34998634-34998653 Msa0404220:intron 55.0%
TGATCCCTATGAGAGGGCCA+AGG - chr3_2:34998636-34998655 Msa0404230:exon 55.0%
!! CTTGAGGGAAGGCGCTACTT+TGG - chr3_2:34999122-34999141 Msa0404230:intron 55.0%
GGGGTAGTCCATTTGTCCTG+AGG - chr3_2:34998410-34998429 Msa0404230:three_prime_UTR 55.0%
GCCCTCTCATAGGGATCATG+TGG + chr3_2:34998634-34998653 Msa0404220:intron 55.0%
TGATCCCTATGAGAGGGCCA+AGG - chr3_2:34998636-34998655 Msa0404230:exon 55.0%
!! CTTGAGGGAAGGCGCTACTT+TGG - chr3_2:34999122-34999141 Msa0404230:intron 55.0%
CAAGAGCCCACTTGACCCTC+AGG + chr3_2:34998429-34998448 Msa0404220:intron 60.0%
! AGAGGGCCAAGGCGCGATTT+TGG - chr3_2:34998647-34998666 Msa0404230:exon 60.0%
! GAGGGCCAAGGCGCGATTTT+GGG - chr3_2:34998648-34998667 Msa0404230:exon 60.0%
!! GAGGGAAGGCGCTACTTTGG+AGG - chr3_2:34999125-34999144 Msa0404230:intron 60.0%
GCACAGCAAGGCCTTCCACA+AGG - chr3_2:34999334-34999353 Msa0404230:intron 60.0%
CAAGAGCCCACTTGACCCTC+AGG + chr3_2:34998429-34998448 Msa0404220:intron 60.0%
! AGAGGGCCAAGGCGCGATTT+TGG - chr3_2:34998647-34998666 Msa0404230:exon 60.0%
! GAGGGCCAAGGCGCGATTTT+GGG - chr3_2:34998648-34998667 Msa0404230:exon 60.0%
!! GAGGGAAGGCGCTACTTTGG+AGG - chr3_2:34999125-34999144 Msa0404230:intron 60.0%
GCACAGCAAGGCCTTCCACA+AGG - chr3_2:34999334-34999353 Msa0404230:intron 60.0%
CGCGCCTTGGCCCTCTCATA+GGG + chr3_2:34998643-34998662 Msa0404220:three_prime_UTR 65.0%
TCGCGCCTTGGCCCTCTCAT+AGG + chr3_2:34998644-34998663 Msa0404220:three_prime_UTR 65.0%
CGCGCCTTGGCCCTCTCATA+GGG + chr3_2:34998643-34998662 Msa0404220:three_prime_UTR 65.0%
TCGCGCCTTGGCCCTCTCAT+AGG + chr3_2:34998644-34998663 Msa0404220:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 34998318 34999712 34998318 ID=Msa0404230;Name=Msa0404230
chr3_2 mRNA 34998318 34999712 34998318 ID=Msa0404230-mRNA-1;Parent=Msa0404230;Name=Msa0404230-mRNA-1;_AED=0.01;_eAED=0.01;_QI=58|1|1|1|1|1|2|333|223
chr3_2 exon 34998318 34999007 34998318 ID=Msa0404230-mRNA-1:exon:7181;Parent=Msa0404230-mRNA-1
chr3_2 exon 34999340 34999712 34999340 ID=Msa0404230-mRNA-1:exon:7180;Parent=Msa0404230-mRNA-1
chr3_2 five_prime_UTR 34999655 34999712 34999655 ID=Msa0404230-mRNA-1:five_prime_utr;Parent=Msa0404230-mRNA-1
chr3_2 CDS 34999340 34999654 34999340 ID=Msa0404230-mRNA-1:cds;Parent=Msa0404230-mRNA-1
chr3_2 CDS 34998651 34999007 34998651 ID=Msa0404230-mRNA-1:cds;Parent=Msa0404230-mRNA-1
chr3_2 three_prime_UTR 34998318 34998650 34998318 ID=Msa0404230-mRNA-1:three_prime_utr;Parent=Msa0404230-mRNA-1
Gene Sequence

>Msa0404230

ATGGCAGGAGACTCACTCACGTTACTAGGGTTCTGGGGTAGTCCATTTGTCCTGAGGGTCAAGTGGGCTCTTGAACTAAAAGGGGTTCAATACCAATATGTTGAAGAAGATTTTAGGAACAAGAGCGATATGCTCTTGCAGTATAATCCTGTTTATAAGAAGGTACCTGTTCTTGTGCATGACGGTAAGTCCATATCAGAATCACTAGTGATTCTAGAGTATGTTGATGAGATCTGGAAGCAAAATCCTCTTCTTCCACATGATCCCTATGAGAGGGCCAAGGCGCGATTTTGGGCTAAATTCGCCGATGATAAGTGTGTGCCAGCATTTATAGCTACCTTTTTCAAAATAGGAGAGGAACAACAGAAGGCTGCACAAGAAGCTCGTGAGAATTTGAAGATCCTTGAAAGTGGACTTGAGGGAAGGCGCTACTTTGGAGGTGAAAATATAGGCTTTGCTGATATTGCTGTTGGGTTTTTTGGATTCTGGGCTCGAGTGATAGAAGAAACTGTAGGCATAAACCTGATTGATACAGAGTCCATGGCTAAACTTACCACTTACTTCGATGACTTTCTTGAGCTTCCTGTTATCAAAGAATGCACGCCGTCTCATGATAAGTTACTTGAGCACAGCAAGGCCTTCCACAAGGTCTTGACATCTTCATCAGCTTAA
Protein sequence

>Msa0404230

MAGDSLTLLGFWGSPFVLRVKWALELKGVQYQYVEEDFRNKSDMLLQYNPVYKKVPVLVHDGKSISESLVILEYVDEIWKQNPLLPHDPYERAKARFWAKFADDKCVPAFIATFFKIGEEQQKAAQEARENLKILESGLEGRRYFGGENIGFADIAVGFFGFWARVIEETVGINLIDTESMAKLTTYFDDFLELPVIKECTPSHDKLLEHSKAFHKVLTSSSA*