Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0452910 | AES70281.2 | 54.167 | 72 | 9 | 1 | 1 | 72 | 315 | 362 | 7.85e-13 | 72.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0452910 | G7J1Z7 | 54.167 | 72 | 9 | 1 | 1 | 72 | 315 | 362 | 3.75e-13 | 72.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0102070 | Msa0452910 | 0.806056 | 9.899532e-50 | -8.615850e-47 |
| Msa0145750 | Msa0452910 | 0.803994 | 2.672587e-49 | -8.615850e-47 |
| Msa0414410 | Msa0452910 | 0.812605 | 3.897952e-51 | -8.615850e-47 |
| Msa0414420 | Msa0452910 | 0.827907 | 1.207053e-54 | -8.615850e-47 |
| Msa0451730 | Msa0452910 | 0.806326 | 8.687375e-50 | -8.615850e-47 |
| Msa0452900 | Msa0452910 | 0.845690 | 3.471095e-59 | -8.615850e-47 |
| Msa0452910 | Msa0564890 | 0.804627 | 1.972535e-49 | -8.615850e-47 |
| Msa0452910 | Msa0726140 | 0.813212 | 2.869413e-51 | -8.615850e-47 |
| Msa0452910 | Msa0743810 | 0.843155 | 1.667667e-58 | -8.615850e-47 |
| Msa0452910 | Msa0745060 | 0.826482 | 2.648279e-54 | -8.615850e-47 |
| Msa0452910 | Msa0783160 | 0.807534 | 4.822864e-50 | -8.615850e-47 |
| Msa0452910 | Msa0808010 | 0.801565 | 8.481124e-49 | -8.615850e-47 |
| Msa0452910 | Msa0825860 | 0.831277 | 1.828680e-55 | -8.615850e-47 |
| Msa0452910 | Msa0846330 | 0.803676 | 3.112126e-49 | -8.615850e-47 |
| Msa0452910 | Msa0865320 | 0.808683 | 2.744972e-50 | -8.615850e-47 |
| Msa0452910 | Msa0887920 | 0.814894 | 1.220939e-51 | -8.615850e-47 |
| Msa0452910 | Msa0888370 | 0.804375 | 2.226855e-49 | -8.615850e-47 |
| Msa0452910 | Msa0989060 | 0.800221 | 1.595399e-48 | -8.615850e-47 |
| Msa0452910 | Msa1016280 | 0.821833 | 3.272346e-53 | -8.615850e-47 |
| Msa0452910 | Msa1025360 | 0.811477 | 6.865070e-51 | -8.615850e-47 |
| Msa0452910 | Msa1025370 | 0.848118 | 7.514550e-60 | -8.615850e-47 |
| Msa0452910 | Msa1044830 | 0.856119 | 3.991390e-62 | -8.615850e-47 |
| Msa0452910 | Msa1044840 | 0.815717 | 8.012957e-52 | -8.615850e-47 |
| Msa0452910 | Msa1100980 | 0.831857 | 1.315667e-55 | -8.615850e-47 |
| Msa0452910 | Msa1109440 | 0.808034 | 3.776945e-50 | -8.615850e-47 |
| Msa0452910 | Msa1121200 | 0.802071 | 6.676538e-49 | -8.615850e-47 |
| Msa0452910 | Msa1135740 | 0.814727 | 1.329380e-51 | -8.615850e-47 |
| Msa0452910 | Msa1146110 | 0.810905 | 9.135156e-51 | -8.615850e-47 |
| Msa0452910 | Msa1150370 | 0.804845 | 1.776672e-49 | -8.615850e-47 |
| Msa0452910 | Msa1187940 | 0.806025 | 1.004863e-49 | -8.615850e-47 |
| Msa0452910 | Msa1192640 | 0.815763 | 7.824265e-52 | -8.615850e-47 |
| Msa0452910 | Msa1193730 | 0.815685 | 8.146794e-52 | -8.615850e-47 |
| Msa0452910 | Msa1246880 | 0.811116 | 8.223390e-51 | -8.615850e-47 |
| Msa0452910 | Msa1269670 | 0.815090 | 1.104801e-51 | -8.615850e-47 |
| Msa0452910 | Msa1270230 | 0.806984 | 6.306071e-50 | -8.615850e-47 |
| Msa0452910 | Msa1270270 | 0.832666 | 8.298411e-56 | -8.615850e-47 |
| Msa0452910 | Msa1283330 | 0.800292 | 1.543071e-48 | -8.615850e-47 |
| Msa0452910 | Msa1285930 | 0.824115 | 9.612474e-54 | -8.615850e-47 |
| Msa0452910 | Msa1287800 | 0.836474 | 9.161203e-57 | -8.615850e-47 |
| Msa0452910 | Msa1287810 | 0.844554 | 7.037181e-59 | -8.615850e-47 |
| Msa0452910 | Msa1287830 | 0.832025 | 1.196086e-55 | -8.615850e-47 |
| Msa0452910 | Msa1340590 | 0.801018 | 1.097456e-48 | -8.615850e-47 |
| Msa0452910 | Msa1342200 | 0.800003 | 1.767322e-48 | -8.615850e-47 |
| Msa0452910 | Msa1404000 | 0.814302 | 1.650662e-51 | -8.615850e-47 |
| Msa0452910 | Msa1415310 | 0.826731 | 2.310164e-54 | -8.615850e-47 |
| Msa0452910 | Msa1433120 | 0.818169 | 2.254986e-52 | -8.615850e-47 |
| Msa0267000 | Msa0452910 | 0.846906 | 1.617599e-59 | -8.615850e-47 |
| Msa0267010 | Msa0452910 | 0.815611 | 8.458337e-52 | -8.615850e-47 |
| Msa0342240 | Msa0452910 | 0.802628 | 5.126351e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0452910 | MtrunA17_Chr4g0038111 | 92.593 | 27 | 2 | 0 | 1 | 27 | 398 | 424 | 1.14e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAATTACCTGTTATTATT+AGG | 0.205492 | 3_3:-42714712 | None:intergenic |
| TTACTTCATGGACTGTTTAC+AGG | 0.256586 | 3_3:+42714861 | Msa0452910:CDS |
| AATACAACATTAGAAGAAAC+AGG | 0.329306 | 3_3:-42714831 | None:intergenic |
| CATATACTCCTAATAATAAC+AGG | 0.390293 | 3_3:+42714704 | Msa0452910:CDS |
| AGGAAAATATACCTTGGTAT+AGG | 0.418282 | 3_3:-42714908 | None:intergenic |
| ATATTAGCACTTAAGATGAA+TGG | 0.425912 | 3_3:+42714628 | None:intergenic |
| ATATACCTTGGTATAGGCCT+GGG | 0.458790 | 3_3:-42714902 | None:intergenic |
| TATTAGCACTTAAGATGAAT+GGG | 0.460579 | 3_3:+42714629 | None:intergenic |
| GACTTGATTTAGCTCGGGAG+AGG | 0.463792 | 3_3:+42714677 | Msa0452910:CDS |
| AATATACCTTGGTATAGGCC+TGG | 0.466149 | 3_3:-42714903 | None:intergenic |
| TGGACTGTTTACAGGCGTGC+AGG | 0.495759 | 3_3:+42714869 | Msa0452910:CDS |
| CTGTCCGACTTGATTTAGCT+CGG | 0.502487 | 3_3:+42714671 | Msa0452910:CDS |
| ACTTGATTTAGCTCGGGAGA+GGG | 0.513360 | 3_3:+42714678 | Msa0452910:CDS |
| TGTCCGACTTGATTTAGCTC+GGG | 0.530721 | 3_3:+42714672 | Msa0452910:CDS |
| CTTGATTTAGCTCGGGAGAG+GGG | 0.578698 | 3_3:+42714679 | Msa0452910:CDS |
| AAAACTGCTACCCTAGTCGT+CGG | 0.600371 | 3_3:-42715156 | None:intergenic |
| GTGCAGGATTCTATAAACCC+AGG | 0.655926 | 3_3:+42714885 | Msa0452910:CDS |
| TCTCCCGAGCTAAATCAAGT+CGG | 0.679997 | 3_3:-42714675 | None:intergenic |
| ATACAACATTAGAAGAAACA+GGG | 0.691600 | 3_3:-42714830 | None:intergenic |
| ATAAACCCAGGCCTATACCA+AGG | 0.692520 | 3_3:+42714897 | Msa0452910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGAAAAAAGTAACAAATAA+TGG | - | chr3_3:42714789-42714808 | None:intergenic | 15.0% |
| !!! | TATTTGTTACTTTTTTCAAT+AGG | + | chr3_3:42714790-42714809 | Msa0452910:intron | 15.0% |
| !! | AGTAATTACCTGTTATTATT+AGG | - | chr3_3:42714715-42714734 | None:intergenic | 20.0% |
| !! | GTAGTTAAAAGAAAATTTCT+TGG | - | chr3_3:42714742-42714761 | None:intergenic | 20.0% |
| !! | GTATTAAAAACTTTACTTCA+TGG | + | chr3_3:42714849-42714868 | Msa0452910:CDS | 20.0% |
| !! | AAATATTGTTGAAAAACCAT+TGG | - | chr3_3:42714959-42714978 | None:intergenic | 20.0% |
| !!! | AATTTAACTTGTCATTTTTC+AGG | + | chr3_3:42715116-42715135 | Msa0452910:intron | 20.0% |
| !!! | ATTTAACTTGTCATTTTTCA+GGG | + | chr3_3:42715117-42715136 | Msa0452910:intron | 20.0% |
| ! | CATATACTCCTAATAATAAC+AGG | + | chr3_3:42714704-42714723 | Msa0452910:CDS | 25.0% |
| ! | ATACAACATTAGAAGAAACA+GGG | - | chr3_3:42714833-42714852 | None:intergenic | 25.0% |
| ! | AATACAACATTAGAAGAAAC+AGG | - | chr3_3:42714834-42714853 | None:intergenic | 25.0% |
| ! | AAAAAGAGGAAAATATACCT+TGG | - | chr3_3:42714917-42714936 | None:intergenic | 25.0% |
| AGGAAAATATACCTTGGTAT+AGG | - | chr3_3:42714911-42714930 | None:intergenic | 30.0% | |
| ! | TTACTTCATGGACTGTTTAC+AGG | + | chr3_3:42714861-42714880 | Msa0452910:CDS | 35.0% |
| CATAGTACAACAGCAAAAAG+AGG | - | chr3_3:42714931-42714950 | None:intergenic | 35.0% | |
| TGAACAACTTCTCGATGAAT+AGG | + | chr3_3:42714981-42715000 | Msa0452910:intron | 35.0% | |
| GAACAACTTCTCGATGAATA+GGG | + | chr3_3:42714982-42715001 | Msa0452910:intron | 35.0% | |
| TAATGGAATCGACACAAGTG+AGG | - | chr3_3:42714772-42714791 | None:intergenic | 40.0% | |
| ATATACCTTGGTATAGGCCT+GGG | - | chr3_3:42714905-42714924 | None:intergenic | 40.0% | |
| AATATACCTTGGTATAGGCC+TGG | - | chr3_3:42714906-42714925 | None:intergenic | 40.0% | |
| GTTGTACTATGTTCTGCCAA+TGG | + | chr3_3:42714940-42714959 | Msa0452910:intron | 40.0% | |
| ! | ATACATTGTTGCTTCAAGCG+TGG | + | chr3_3:42715077-42715096 | Msa0452910:intron | 40.0% |
| ! | CTGTCCGACTTGATTTAGCT+CGG | + | chr3_3:42714671-42714690 | Msa0452910:CDS | 45.0% |
| ! | TGTCCGACTTGATTTAGCTC+GGG | + | chr3_3:42714672-42714691 | Msa0452910:CDS | 45.0% |
| TCTCCCGAGCTAAATCAAGT+CGG | - | chr3_3:42714678-42714697 | None:intergenic | 45.0% | |
| ! | ACTTGATTTAGCTCGGGAGA+GGG | + | chr3_3:42714678-42714697 | Msa0452910:CDS | 45.0% |
| GTGCAGGATTCTATAAACCC+AGG | + | chr3_3:42714885-42714904 | Msa0452910:CDS | 45.0% | |
| ATAAACCCAGGCCTATACCA+AGG | + | chr3_3:42714897-42714916 | Msa0452910:CDS | 45.0% | |
| ! | GACTTGATTTAGCTCGGGAG+AGG | + | chr3_3:42714677-42714696 | Msa0452910:CDS | 50.0% |
| ! | CTTGATTTAGCTCGGGAGAG+GGG | + | chr3_3:42714679-42714698 | Msa0452910:CDS | 50.0% |
| TGGACTGTTTACAGGCGTGC+AGG | + | chr3_3:42714869-42714888 | Msa0452910:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 42714643 | 42715166 | 42714643 | ID=Msa0452910;Name=Msa0452910 |
| chr3_3 | mRNA | 42714643 | 42715166 | 42714643 | ID=Msa0452910-mRNA-1;Parent=Msa0452910;Name=Msa0452910-mRNA-1;_AED=0.71;_eAED=0.76;_QI=0|0|0|0.66|1|1|3|0|72 |
| chr3_3 | exon | 42714643 | 42714725 | 42714643 | ID=Msa0452910-mRNA-1:exon:7717;Parent=Msa0452910-mRNA-1 |
| chr3_3 | exon | 42714812 | 42714918 | 42714812 | ID=Msa0452910-mRNA-1:exon:7718;Parent=Msa0452910-mRNA-1 |
| chr3_3 | exon | 42715138 | 42715166 | 42715138 | ID=Msa0452910-mRNA-1:exon:7719;Parent=Msa0452910-mRNA-1 |
| chr3_3 | CDS | 42714643 | 42714725 | 42714643 | ID=Msa0452910-mRNA-1:cds;Parent=Msa0452910-mRNA-1 |
| chr3_3 | CDS | 42714812 | 42714918 | 42714812 | ID=Msa0452910-mRNA-1:cds;Parent=Msa0452910-mRNA-1 |
| chr3_3 | CDS | 42715138 | 42715166 | 42715138 | ID=Msa0452910-mRNA-1:cds;Parent=Msa0452910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
