AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1246880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1246880 MtrunA17_Chr5g0442971 60.526 114 29 1 6 103 424 537 1.07e-34 126
Msa1246880 MtrunA17_Chr5g0443151 57.658 111 27 2 6 96 175 285 1.72e-28 108
Msa1246880 MtrunA17_Chr5g0443141 62.500 64 24 0 6 69 3 66 1.08e-19 82.4
Msa1246880 MtrunA17_Chr5g0443181 51.852 81 39 0 6 86 272 352 1.17e-18 79.7
Msa1246880 MtrunA17_Chr5g0439971 60.784 51 20 0 6 56 772 822 9.23e-14 66.6
Msa1246880 MtrunA17_Chr3g0119031 55.000 60 26 1 6 64 767 826 5.40e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTGTAGTAGAAGCTTTC+TGG 0.175123 8_2:+38997419 None:intergenic
TTCCAATGATTCTTCATCTT+TGG 0.181692 8_2:+38997181 None:intergenic
CCATGAATGCAGTTAGAAAA+TGG 0.255703 8_2:-38997457 Msa1246880:intron
TCATTAGCAGAGGAAGAAAA+AGG 0.334938 8_2:+38997206 None:intergenic
TTCTCATGTGCCTTTATACT+TGG 0.341087 8_2:-38997377 Msa1246880:CDS
CAATTCTGCTCCAACTCCTT+TGG 0.341180 8_2:+38995992 None:intergenic
TGCACATATTCCAAGTATAA+AGG 0.396963 8_2:+38997367 None:intergenic
AACCAAAGATGAAGAATCAT+TGG 0.407579 8_2:-38997183 Msa1246880:CDS
AAATTCCACACCAAAGGAGT+TGG 0.477307 8_2:-38996002 Msa1246880:CDS
GGAATATGTGCACAATCGCC+AGG 0.502785 8_2:-38997356 Msa1246880:intron
GCTGACTAATATTGAAGTGT+TGG 0.508117 8_2:+38997112 None:intergenic
AATATTAGTCAGCATAGACT+TGG 0.541731 8_2:-38997102 Msa1246880:CDS
ATCTTTGGTTTCATTAGCAG+AGG 0.553118 8_2:+38997196 None:intergenic
CTGCTCCAACTCCTTTGGTG+TGG 0.581033 8_2:+38995997 None:intergenic
AGTGTTGGCTTCATCATGAG+TGG 0.599112 8_2:+38997127 None:intergenic
AGTTTCAAATTCCACACCAA+AGG 0.600607 8_2:-38996008 Msa1246880:CDS
GTGGAATTTGAAACTCTCAA+AGG 0.634403 8_2:+38996016 None:intergenic
ATAAAGGCACATGAGAATAG+CGG 0.661853 8_2:+38997383 None:intergenic
AATTGATGCTATGATAGACG+AGG 0.695057 8_2:-38997075 Msa1246880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAGAAGATTTAAGA+GGG + chr8_2:38996200-38996219 None:intergenic 15.0%
!! ATAAAATAAGAAGATTTAAG+AGG + chr8_2:38996201-38996220 None:intergenic 15.0%
!!! AATAAATTTTATGTCAACTA+AGG + chr8_2:38996501-38996520 None:intergenic 15.0%
!! ATAAAATTTATTTCCTTTGA+TGG - chr8_2:38996509-38996528 Msa1246880:intron 15.0%
!! TTTATTATTTATCTGTTGTA+TGG - chr8_2:38996662-38996681 Msa1246880:intron 15.0%
!!! ATTTTCTTTTTCATGTATAA+AGG - chr8_2:38997164-38997183 Msa1246880:CDS 15.0%
!! AGCATAAAACATAAATTAGA+GGG + chr8_2:38996298-38996317 None:intergenic 20.0%
!! TGTTAATATAGTGATTATCA+TGG - chr8_2:38996347-38996366 Msa1246880:intron 20.0%
!!! TTGCATTAGATTTCATTTTT+TGG - chr8_2:38996374-38996393 Msa1246880:intron 20.0%
!!! TGCATTAGATTTCATTTTTT+GGG - chr8_2:38996375-38996394 Msa1246880:intron 20.0%
!!! ATTTATTCATTCCTTTTTCT+AGG - chr8_2:38997230-38997249 Msa1246880:intron 20.0%
!! TAAAAGTAATTTCAGAAACT+TGG - chr8_2:38997507-38997526 Msa1246880:intron 20.0%
! AAGTATAATTGAAAAGCTTC+AGG + chr8_2:38996166-38996185 None:intergenic 25.0%
! CAGCATAAAACATAAATTAG+AGG + chr8_2:38996299-38996318 None:intergenic 25.0%
! AATATAGTGATTATCATGGT+TGG - chr8_2:38996351-38996370 Msa1246880:intron 25.0%
!!! TGTGATTTTGACATATATTG+AGG - chr8_2:38996464-38996483 Msa1246880:intron 25.0%
! TTGACATATATTGAGGTAAA+AGG - chr8_2:38996471-38996490 Msa1246880:intron 25.0%
! CTTAACAAGGTTAAATAAAC+TGG - chr8_2:38996583-38996602 Msa1246880:intron 25.0%
!!! ATAAGCTTGCTAGTAATTAA+TGG - chr8_2:38996733-38996752 Msa1246880:intron 25.0%
! AACAATAGGCATAATGAATT+CGG + chr8_2:38996886-38996905 None:intergenic 25.0%
! AATGTTAATTTCTTGTCAAC+AGG + chr8_2:38996948-38996967 None:intergenic 25.0%
! ATTAACATTTGAAGAAAGTG+AGG - chr8_2:38996959-38996978 Msa1246880:intron 25.0%
! TACATGAAAAAGAAAATACC+TGG + chr8_2:38997161-38997180 None:intergenic 25.0%
!! TAAATGTGTAGCTTTCATTT+TGG + chr8_2:38997215-38997234 None:intergenic 25.0%
! TAGTTCTTACAGTAATGAAA+TGG + chr8_2:38997489-38997508 None:intergenic 25.0%
! TTAAAACACTCTAACAAGTA+TGG - chr8_2:38997645-38997664 Msa1246880:intron 25.0%
! TAAAACACTCTAACAAGTAT+GGG - chr8_2:38997646-38997665 Msa1246880:intron 25.0%
! TCTCATTATAGAAGTTTGAT+TGG + chr8_2:38998434-38998453 None:intergenic 25.0%
ATTGCTAACATGTGATATGT+AGG - chr8_2:38996005-38996024 Msa1246880:CDS 30.0%
AGATAGAATTAACTGTTGAC+CGG + chr8_2:38996402-38996421 None:intergenic 30.0%
!! GTTAGCTTCTTCTTTTACTA+AGG - chr8_2:38996424-38996443 Msa1246880:intron 30.0%
ACATATATTGAGGTAAAAGG+TGG - chr8_2:38996474-38996493 Msa1246880:intron 30.0%
TCAAACATAACTTCCATCAA+AGG + chr8_2:38996525-38996544 None:intergenic 30.0%
TTGATGGAAGTTATGTTTGA+AGG - chr8_2:38996525-38996544 Msa1246880:intron 30.0%
AACCTTGTTAAGATTTCTGA+CGG + chr8_2:38996575-38996594 None:intergenic 30.0%
TTGCATATGACTTACCATAA+GGG - chr8_2:38996795-38996814 Msa1246880:intron 30.0%
TAAAAAGTTCCTTTCCCTTA+TGG + chr8_2:38996812-38996831 None:intergenic 30.0%
TGCACATATTCCAAGTATAA+AGG + chr8_2:38997132-38997151 None:intergenic 30.0%
! ATAAAGGCAATTAGTTTCGT+CGG - chr8_2:38997180-38997199 Msa1246880:CDS 30.0%
! TTCAAGTTTAGCCTAGAAAA+AGG + chr8_2:38997244-38997263 None:intergenic 30.0%
AACCAAAGATGAAGAATCAT+TGG - chr8_2:38997313-38997332 Msa1246880:intron 30.0%
TTCCAATGATTCTTCATCTT+TGG + chr8_2:38997318-38997337 None:intergenic 30.0%
AATATTAGTCAGCATAGACT+TGG - chr8_2:38997394-38997413 Msa1246880:CDS 30.0%
!!! GTTTTAAGACTTTGTAACCT+TGG + chr8_2:38997632-38997651 None:intergenic 30.0%
!! AACTTTTCAAACTTAGCTCT+TGG - chr8_2:38997819-38997838 Msa1246880:intron 30.0%
AGGAAAATACTCTATGACTT+GGG + chr8_2:38998047-38998066 None:intergenic 30.0%
! AGTGAAAATGGAAGTTTGTT+AGG + chr8_2:38998251-38998270 None:intergenic 30.0%
! AACAAACTTCCATTTTCACT+TGG - chr8_2:38998251-38998270 Msa1246880:intron 30.0%
! GTACTACTCAATTTGGAATA+TGG + chr8_2:38998283-38998302 None:intergenic 30.0%
! AGGGTACTTAGATATGTTAA+GGG + chr8_2:38998305-38998324 None:intergenic 30.0%
AACATGTGATATGTAGGAAG+AGG - chr8_2:38996011-38996030 Msa1246880:CDS 35.0%
! GAAGAGGATGCTAAATCTTT+TGG - chr8_2:38996027-38996046 Msa1246880:CDS 35.0%
TTCCTCAACATGTGTGTTAA+TGG + chr8_2:38996117-38996136 None:intergenic 35.0%
!!! ATCTCTGCTTGTTAGCTTTT+AGG - chr8_2:38996230-38996249 Msa1246880:intron 35.0%
!!! TAGATTTCATTTTTTGGGCC+CGG - chr8_2:38996380-38996399 Msa1246880:intron 35.0%
GATAGAATTAACTGTTGACC+GGG + chr8_2:38996401-38996420 None:intergenic 35.0%
ACAAGGTTAAATAAACTGGC+TGG - chr8_2:38996587-38996606 Msa1246880:intron 35.0%
TAAAGTTCAATTCCTTCGTC+AGG + chr8_2:38996620-38996639 None:intergenic 35.0%
CGAAGGAATTGAACTTTATG+AGG - chr8_2:38996622-38996641 Msa1246880:intron 35.0%
GGAATTGAACTTTATGAGGT+TGG - chr8_2:38996626-38996645 Msa1246880:intron 35.0%
CTTGCATATGACTTACCATA+AGG - chr8_2:38996794-38996813 Msa1246880:intron 35.0%
TATGACTTACCATAAGGGAA+AGG - chr8_2:38996800-38996819 Msa1246880:intron 35.0%
! AGGGAAAGGAACTTTTTATC+AGG - chr8_2:38996814-38996833 Msa1246880:intron 35.0%
TTATAAGTCACTCAGAGATC+AGG + chr8_2:38996850-38996869 None:intergenic 35.0%
ATAATCTCCTGCACAACAAT+AGG + chr8_2:38996900-38996919 None:intergenic 35.0%
CATTTGAAGAAAGTGAGGTA+TGG - chr8_2:38996964-38996983 Msa1246880:intron 35.0%
CAATTATACTGTGCATCAAG+CGG + chr8_2:38996997-38997016 None:intergenic 35.0%
! CCATTTTCTAACTGCATTCA+TGG + chr8_2:38997042-38997061 None:intergenic 35.0%
CCATGAATGCAGTTAGAAAA+TGG - chr8_2:38997039-38997058 Msa1246880:intron 35.0%
TATCTGTAGTAGAAGCTTTC+TGG + chr8_2:38997080-38997099 None:intergenic 35.0%
! ATAAAGGCACATGAGAATAG+CGG + chr8_2:38997116-38997135 None:intergenic 35.0%
TTCTCATGTGCCTTTATACT+TGG - chr8_2:38997119-38997138 Msa1246880:CDS 35.0%
!!! TGAACGTTTTTTCCTTGTTC+AGG - chr8_2:38997265-38997284 Msa1246880:intron 35.0%
!!! GAACGTTTTTTCCTTGTTCA+GGG - chr8_2:38997266-38997285 Msa1246880:intron 35.0%
TCATTAGCAGAGGAAGAAAA+AGG + chr8_2:38997293-38997312 None:intergenic 35.0%
! ATCTTTGGTTTCATTAGCAG+AGG + chr8_2:38997303-38997322 None:intergenic 35.0%
GCTGACTAATATTGAAGTGT+TGG + chr8_2:38997387-38997406 None:intergenic 35.0%
AATTGATGCTATGATAGACG+AGG - chr8_2:38997421-38997440 Msa1246880:CDS 35.0%
!! ATCGACAGAGTTTTATCAGA+AGG - chr8_2:38997458-38997477 Msa1246880:intron 35.0%
!! TCGACAGAGTTTTATCAGAA+GGG - chr8_2:38997459-38997478 Msa1246880:intron 35.0%
AAACAGAAAAAGTGAACAGC+AGG - chr8_2:38997582-38997601 Msa1246880:intron 35.0%
AAGACTTTGTAACCTTGGTT+AGG + chr8_2:38997627-38997646 None:intergenic 35.0%
GAAAAGTTTCGAGAATTGCT+TGG + chr8_2:38997807-38997826 None:intergenic 35.0%
GTACTTCACTTGAATATGCT+TGG - chr8_2:38997935-38997954 Msa1246880:intron 35.0%
!! TTGGTTTTTCCATGTTGAAC+TGG - chr8_2:38997954-38997973 Msa1246880:intron 35.0%
GAGGAAAATACTCTATGACT+TGG + chr8_2:38998048-38998067 None:intergenic 35.0%
! TTTTCCTCAACCAAATAGCT+TGG - chr8_2:38998060-38998079 Msa1246880:intron 35.0%
AGAAATTGTAGCTCAATCCA+CGG + chr8_2:38998123-38998142 None:intergenic 35.0%
!!! TATGCTTTTTCTTTAGGCTC+TGG + chr8_2:38998173-38998192 None:intergenic 35.0%
!!! ACAGGCTATGCTTTTTCTTT+AGG + chr8_2:38998179-38998198 None:intergenic 35.0%
!! AGGCTATGTTGATAGTGATT+GGG + chr8_2:38998231-38998250 None:intergenic 35.0%
!! TAGGCTATGTTGATAGTGAT+TGG + chr8_2:38998232-38998251 None:intergenic 35.0%
!! GTTAAGGGTACTACTCAATT+TGG + chr8_2:38998290-38998309 None:intergenic 35.0%
!! GAGGGTACTTAGATATGTTA+AGG + chr8_2:38998306-38998325 None:intergenic 35.0%
CTTGTTGCATACATAAGATC+AGG - chr8_2:38998380-38998399 Msa1246880:intron 35.0%
! GTGGAATTTGAAACTCTCAA+AGG + chr8_2:38998483-38998502 None:intergenic 35.0%
AGTTTCAAATTCCACACCAA+AGG - chr8_2:38998488-38998507 Msa1246880:intron 35.0%
!! AAAAGTGTTTGGGATTGAGC+TGG + chr8_2:38996057-38996076 None:intergenic 40.0%
! AGCTCAATCCCAAACACTTT+TGG - chr8_2:38996056-38996075 Msa1246880:intron 40.0%
! GCTCAATCCCAAACACTTTT+GGG - chr8_2:38996057-38996076 Msa1246880:intron 40.0%
!! TCCCAAACACTTTTGGGTAT+TGG - chr8_2:38996063-38996082 Msa1246880:intron 40.0%
!! GCCAATACCCAAAAGTGTTT+GGG + chr8_2:38996067-38996086 None:intergenic 40.0%
!! TGCCAATACCCAAAAGTGTT+TGG + chr8_2:38996068-38996087 None:intergenic 40.0%
GTCCATTAACACACATGTTG+AGG - chr8_2:38996112-38996131 Msa1246880:intron 40.0%
AGGCATTCTCATGAACTTAC+AGG + chr8_2:38996146-38996165 None:intergenic 40.0%
!!! GTTAGCTTTTAGGCAAATGC+TGG - chr8_2:38996240-38996259 Msa1246880:intron 40.0%
AGCCGTCAGAAATCTTAACA+AGG - chr8_2:38996570-38996589 Msa1246880:intron 40.0%
CATTATGCCTATTGTTGTGC+AGG - chr8_2:38996890-38996909 Msa1246880:intron 40.0%
CGCTTGATGCACAGTATAAT+TGG - chr8_2:38996995-38997014 Msa1246880:intron 40.0%
AAGAAAAAGGTCCCTGAACA+AGG + chr8_2:38997280-38997299 None:intergenic 40.0%
AAGTTACCTCTGCAGAAGTA+TGG + chr8_2:38997675-38997694 None:intergenic 40.0%
! AACACTCCTCCTTGAGATTT+TGG - chr8_2:38997772-38997791 Msa1246880:intron 40.0%
AGTGTCCATCCAAAATCTCA+AGG + chr8_2:38997784-38997803 None:intergenic 40.0%
GCTGAAAATCCAGTTCAACA+TGG + chr8_2:38997966-38997985 None:intergenic 40.0%
CAAACCAAGCTATTTGGTTG+AGG + chr8_2:38998067-38998086 None:intergenic 40.0%
!! GATGACATGAAGAGTACTAC+AGG + chr8_2:38998197-38998216 None:intergenic 40.0%
!! TATGTTGATAGTGATTGGGC+TGG + chr8_2:38998227-38998246 None:intergenic 40.0%
TGGTACAAGCCAAGTGAAAA+TGG + chr8_2:38998263-38998282 None:intergenic 40.0%
! ACATTTTGCTGCAGCAAAGA+GGG + chr8_2:38998324-38998343 None:intergenic 40.0%
AAATTCCACACCAAAGGAGT+TGG - chr8_2:38998494-38998513 Msa1246880:intron 40.0%
AACACACATGTTGAGGAAGC+AGG - chr8_2:38996119-38996138 Msa1246880:intron 45.0%
ACAGGCTCACACATGACATT+TGG + chr8_2:38996930-38996949 None:intergenic 45.0%
!! AGTGTTGGCTTCATCATGAG+TGG + chr8_2:38997372-38997391 None:intergenic 45.0%
AGTAGTCTGTCACCTAACCA+AGG - chr8_2:38997612-38997631 Msa1246880:intron 45.0%
! CTCCTCCTTGAGATTTTGGA+TGG - chr8_2:38997776-38997795 Msa1246880:intron 45.0%
GTCCATCCAAAATCTCAAGG+AGG + chr8_2:38997781-38997800 None:intergenic 45.0%
! ATTTTTGACAGCCTCGCGTA+TGG - chr8_2:38997908-38997927 Msa1246880:intron 45.0%
! TGACTTGGGAATACAACAGC+TGG + chr8_2:38998033-38998052 None:intergenic 45.0%
CAGCAGCAAACCAAGCTATT+TGG + chr8_2:38998073-38998092 None:intergenic 45.0%
! CACATTTTGCTGCAGCAAAG+AGG + chr8_2:38998325-38998344 None:intergenic 45.0%
TGCTGCAGCAAAATGTGTCT+TGG - chr8_2:38998328-38998347 Msa1246880:intron 45.0%
GCAGCAAAATGTGTCTTGGT+AGG - chr8_2:38998332-38998351 Msa1246880:intron 45.0%
CAATTCTGCTCCAACTCCTT+TGG + chr8_2:38998507-38998526 None:intergenic 45.0%
GGAATATGTGCACAATCGCC+AGG - chr8_2:38997140-38997159 Msa1246880:CDS 50.0%
GCATGTTCTCATGGAACTCG+AGG + chr8_2:38998151-38998170 None:intergenic 50.0%
! TAGGCTCTGGCATGTTCTCA+TGG + chr8_2:38998160-38998179 None:intergenic 50.0%
GCTGGTTACGATCCTGACGA+AGG - chr8_2:38996605-38996624 Msa1246880:intron 55.0%
GGGTCACCATACTTCTGCAG+AGG - chr8_2:38997666-38997685 Msa1246880:intron 55.0%
! GAAGTACCATGCCATACGCG+AGG + chr8_2:38997922-38997941 None:intergenic 55.0%
!! TTGGTTTGCTGCTGCAGCTG+CGG - chr8_2:38998079-38998098 Msa1246880:intron 55.0%
TGTAGCTCAATCCACGGCTG+AGG + chr8_2:38998117-38998136 None:intergenic 55.0%
CTGCTCCAACTCCTTTGGTG+TGG + chr8_2:38998502-38998521 None:intergenic 55.0%
TGACAGCCTCGCGTATGGCA+TGG - chr8_2:38997913-38997932 Msa1246880:intron 60.0%
TACGTACTCTGCCTCAGCCG+TGG - chr8_2:38998103-38998122 Msa1246880:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 38995976 38998542 38995976 ID=Msa1246880;Name=Msa1246880
chr8_2 mRNA 38995976 38998542 38995976 ID=Msa1246880-mRNA-1;Parent=Msa1246880;Name=Msa1246880-mRNA-1;_AED=0.57;_eAED=0.60;_QI=0|0|0.2|0.4|1|1|5|0|125
chr8_2 exon 38998536 38998542 38998536 ID=Msa1246880-mRNA-1:exon:12094;Parent=Msa1246880-mRNA-1
chr8_2 exon 38997599 38997606 38997599 ID=Msa1246880-mRNA-1:exon:12093;Parent=Msa1246880-mRNA-1
chr8_2 exon 38997357 38997467 38997357 ID=Msa1246880-mRNA-1:exon:12092;Parent=Msa1246880-mRNA-1
chr8_2 exon 38997052 38997231 38997052 ID=Msa1246880-mRNA-1:exon:12091;Parent=Msa1246880-mRNA-1
chr8_2 exon 38995976 38996047 38995976 ID=Msa1246880-mRNA-1:exon:12090;Parent=Msa1246880-mRNA-1
chr8_2 CDS 38998536 38998542 38998536 ID=Msa1246880-mRNA-1:cds;Parent=Msa1246880-mRNA-1
chr8_2 CDS 38997599 38997606 38997599 ID=Msa1246880-mRNA-1:cds;Parent=Msa1246880-mRNA-1
chr8_2 CDS 38997357 38997467 38997357 ID=Msa1246880-mRNA-1:cds;Parent=Msa1246880-mRNA-1
chr8_2 CDS 38997052 38997231 38997052 ID=Msa1246880-mRNA-1:cds;Parent=Msa1246880-mRNA-1
chr8_2 CDS 38995976 38996047 38995976 ID=Msa1246880-mRNA-1:cds;Parent=Msa1246880-mRNA-1
Gene Sequence

>Msa1246880

ATGATTAGAGATTATTTAGAAAATGGTGATATTATATGCTTCCAGAAAGCTTCTACTACAGATAATGAGAAACATTTCCGCTATTCTCATGTGCCTTTATACTTGGAATATGTGCACAATCGCCAGGGACCTTTTTCTTCCTCTGCTAATGAAACCAAAGATGAAGAATCATTGGAAGAGCAAAACAAATATATTATAGCTGAAGAAACCACTCATGATGAAGCCAACACTTCAATATTAGTCAGCATAGACTTGGAAGAAATTGATGCTATGATAGACGAGGATGTCATTGCTGCAATCGACAGACTTCATCTTCCTTTGAGAGTTTCAAATTCCACACCAAAGGAGTTGGAGCAGAATTGCAGCTTTCCATTTTAA

Protein sequence

>Msa1246880

MIRDYLENGDIICFQKASTTDNEKHFRYSHVPLYLEYVHNRQGPFSSSANETKDEESLEEQNKYIIAEETTHDEANTSILVSIDLEEIDAMIDEDVIAAIDRLHLPLRVSNSTPKELEQNCSFPF*