AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0500740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0500740 MtrunA17_Chr3g0133791 98.913 92 1 0 1 92 1 92 1.26e-58 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0500740 AT5G42700.1 47.312 93 48 1 1 92 2 94 6.97e-15 67.4
Msa0500740 AT3G19184.2 53.571 84 35 3 1 81 1 83 9.44e-12 59.3
Msa0500740 AT3G19184.1 53.571 84 35 3 1 81 1 83 1.03e-11 59.3

Find 24 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTGAAATTCTATTTCT+AGG 0.204642 3_4:-41294517 None:intergenic
ACAACGAGCTCTTTCTTGAT+AGG 0.331075 3_4:-41294331 None:intergenic
TCTCTCTACTCAAATCAAAA+TGG 0.365878 3_4:-41294094 None:intergenic
ACTCAAAATCAAAATCAAAA+TGG 0.367777 3_4:+41294156 Msa0500740:exon
TTACAGACTTCTTTGTAAAC+AGG 0.370188 3_4:-41294391 None:intergenic
CTTGATAGGGGTGCGAGGTT+TGG 0.379420 3_4:-41294317 None:intergenic
CTCGTTGTTGTTCGAAGATC+CGG 0.408143 3_4:+41294346 Msa0500740:CDS
TACCTAGAAATAGAATTTCA+AGG 0.423170 3_4:+41294515 Msa0500740:CDS
AGAGAGTCGCCGGAAAAGAA+TGG 0.454260 3_4:+41294201 Msa0500740:CDS
CAACGAGCTCTTTCTTGATA+GGG 0.456259 3_4:-41294330 None:intergenic
GGAAGAAAATCAAAAGAGAA+TGG 0.470843 3_4:+41294222 Msa0500740:CDS
GTGAAGTGATTGAGAGAGCT+TGG 0.490518 3_4:-41294260 None:intergenic
ACTACACAGAGCTGAGAACT+TGG 0.499295 3_4:+41294126 Msa0500740:five_prime_UTR
GTAAACAGGAGCAGGCATAT+TGG 0.504680 3_4:-41294377 None:intergenic
GGTGCGAGGTTTGGAAGGTG+AGG 0.511953 3_4:-41294308 None:intergenic
ACTTCTTTGTAAACAGGAGC+AGG 0.521856 3_4:-41294385 None:intergenic
AGGCATATTGGCGACACGAC+CGG 0.537317 3_4:-41294365 None:intergenic
GTGCGAGGTTTGGAAGGTGA+GGG 0.543398 3_4:-41294307 None:intergenic
ATAGGGGTGCGAGGTTTGGA+AGG 0.572029 3_4:-41294313 None:intergenic
AACGAGCTCTTTCTTGATAG+GGG 0.578112 3_4:-41294329 None:intergenic
CATCATACGAAGAGAGTCGC+CGG 0.603151 3_4:+41294191 Msa0500740:CDS
CAAAATCAAAATCAAAATGG+TGG 0.614271 3_4:+41294159 Msa0500740:exon
GAGTGGAATGGGAAACGCAA+CGG 0.634670 3_4:+41294053 None:intergenic
TCTTTCTTGATAGGGGTGCG+AGG 0.664811 3_4:-41294322 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTATAATTTTTTTCAAT+AGG - chr3_4:41295116-41295135 None:intergenic 10.0%
!! AAGAAAAATGTATAAATAAA+GGG - chr3_4:41295366-41295385 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - chr3_4:41295367-41295386 None:intergenic 10.0%
!!! TTTGTATAATTTTTTTCAAT+AGG - chr3_4:41295116-41295135 None:intergenic 10.0%
!! AAGAAAAATGTATAAATAAA+GGG - chr3_4:41295366-41295385 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - chr3_4:41295367-41295386 None:intergenic 10.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr3_4:41295301-41295320 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr3_4:41295302-41295321 None:intergenic 15.0%
!! AAAGTGAAATATATTGTATT+GGG - chr3_4:41295342-41295361 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - chr3_4:41295365-41295384 None:intergenic 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr3_4:41295301-41295320 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr3_4:41295302-41295321 None:intergenic 15.0%
!! AAAGTGAAATATATTGTATT+GGG - chr3_4:41295342-41295361 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - chr3_4:41295365-41295384 None:intergenic 15.0%
!! ACTCAAAATCAAAATCAAAA+TGG + chr3_4:41294156-41294175 Msa0500740:exon 20.0%
!!! TCTATTTCTAGGTATTTTAA+CGG - chr3_4:41294509-41294528 None:intergenic 20.0%
!!! AATCTTCTACAACTAAATTT+TGG - chr3_4:41294688-41294707 None:intergenic 20.0%
!! TATGTGTCATAAAAAAAAAG+TGG - chr3_4:41294754-41294773 None:intergenic 20.0%
!! AAATTCACAATGATAATAGT+TGG - chr3_4:41294817-41294836 None:intergenic 20.0%
!! TGAATTTCATAAAACGAAAT+CGG + chr3_4:41294830-41294849 Msa0500740:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - chr3_4:41295232-41295251 None:intergenic 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG - chr3_4:41295268-41295287 None:intergenic 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG - chr3_4:41295269-41295288 None:intergenic 20.0%
!! GAAAGTGAAATATATTGTAT+TGG - chr3_4:41295343-41295362 None:intergenic 20.0%
!! ACTCAAAATCAAAATCAAAA+TGG + chr3_4:41294156-41294175 Msa0500740:exon 20.0%
!!! TCTATTTCTAGGTATTTTAA+CGG - chr3_4:41294509-41294528 None:intergenic 20.0%
!!! AATCTTCTACAACTAAATTT+TGG - chr3_4:41294688-41294707 None:intergenic 20.0%
!! TATGTGTCATAAAAAAAAAG+TGG - chr3_4:41294754-41294773 None:intergenic 20.0%
!! AAATTCACAATGATAATAGT+TGG - chr3_4:41294817-41294836 None:intergenic 20.0%
!! TGAATTTCATAAAACGAAAT+CGG + chr3_4:41294830-41294849 Msa0500740:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - chr3_4:41295232-41295251 None:intergenic 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG - chr3_4:41295268-41295287 None:intergenic 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG - chr3_4:41295269-41295288 None:intergenic 20.0%
!! GAAAGTGAAATATATTGTAT+TGG - chr3_4:41295343-41295362 None:intergenic 20.0%
! CAAAATCAAAATCAAAATGG+TGG + chr3_4:41294159-41294178 Msa0500740:exon 25.0%
! AGTAATAGAGAAAGATGAAA+TGG - chr3_4:41294439-41294458 None:intergenic 25.0%
! TACCTAGAAATAGAATTTCA+AGG + chr3_4:41294515-41294534 Msa0500740:CDS 25.0%
!! AACCTTGAAATTCTATTTCT+AGG - chr3_4:41294520-41294539 None:intergenic 25.0%
! GATGGAAATGAAAAATGATA+AGG + chr3_4:41294942-41294961 Msa0500740:intron 25.0%
!! CAGTGAAAGTTTTCATTAAA+TGG + chr3_4:41295079-41295098 Msa0500740:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - chr3_4:41295233-41295252 None:intergenic 25.0%
!!! CATTGATTTTGTTATTCTGT+AGG + chr3_4:41295508-41295527 Msa0500740:intron 25.0%
! CAAAATCAAAATCAAAATGG+TGG + chr3_4:41294159-41294178 Msa0500740:exon 25.0%
! AGTAATAGAGAAAGATGAAA+TGG - chr3_4:41294439-41294458 None:intergenic 25.0%
! TACCTAGAAATAGAATTTCA+AGG + chr3_4:41294515-41294534 Msa0500740:CDS 25.0%
!! AACCTTGAAATTCTATTTCT+AGG - chr3_4:41294520-41294539 None:intergenic 25.0%
! GATGGAAATGAAAAATGATA+AGG + chr3_4:41294942-41294961 Msa0500740:intron 25.0%
!! CAGTGAAAGTTTTCATTAAA+TGG + chr3_4:41295079-41295098 Msa0500740:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - chr3_4:41295233-41295252 None:intergenic 25.0%
!!! CATTGATTTTGTTATTCTGT+AGG + chr3_4:41295508-41295527 Msa0500740:intron 25.0%
TCTCTCTACTCAAATCAAAA+TGG - chr3_4:41294097-41294116 None:intergenic 30.0%
! GATTTTCTTCCATTCTTTTC+CGG - chr3_4:41294213-41294232 None:intergenic 30.0%
GGAAGAAAATCAAAAGAGAA+TGG + chr3_4:41294222-41294241 Msa0500740:CDS 30.0%
TTACAGACTTCTTTGTAAAC+AGG - chr3_4:41294394-41294413 None:intergenic 30.0%
AAAGATGAAATGGTGAATGT+GGG - chr3_4:41294429-41294448 None:intergenic 30.0%
!!! CACAATAGTGTTTTAGGAAA+CGG - chr3_4:41294897-41294916 None:intergenic 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG - chr3_4:41295254-41295273 None:intergenic 30.0%
! TTGAAATGGGTCAGTTATTT+TGG - chr3_4:41295255-41295274 None:intergenic 30.0%
AGCCAAGATGTCAAATAAAT+TGG + chr3_4:41295401-41295420 Msa0500740:intron 30.0%
GCCAAGATGTCAAATAAATT+GGG + chr3_4:41295402-41295421 Msa0500740:intron 30.0%
TCCCAATTTATTTGACATCT+TGG - chr3_4:41295406-41295425 None:intergenic 30.0%
AGATGTCAAATAAATTGGGA+CGG + chr3_4:41295406-41295425 Msa0500740:intron 30.0%
TCTCTCTACTCAAATCAAAA+TGG - chr3_4:41294097-41294116 None:intergenic 30.0%
! GATTTTCTTCCATTCTTTTC+CGG - chr3_4:41294213-41294232 None:intergenic 30.0%
GGAAGAAAATCAAAAGAGAA+TGG + chr3_4:41294222-41294241 Msa0500740:CDS 30.0%
TTACAGACTTCTTTGTAAAC+AGG - chr3_4:41294394-41294413 None:intergenic 30.0%
AAAGATGAAATGGTGAATGT+GGG - chr3_4:41294429-41294448 None:intergenic 30.0%
!!! CACAATAGTGTTTTAGGAAA+CGG - chr3_4:41294897-41294916 None:intergenic 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG - chr3_4:41295254-41295273 None:intergenic 30.0%
! TTGAAATGGGTCAGTTATTT+TGG - chr3_4:41295255-41295274 None:intergenic 30.0%
AGCCAAGATGTCAAATAAAT+TGG + chr3_4:41295401-41295420 Msa0500740:intron 30.0%
GCCAAGATGTCAAATAAATT+GGG + chr3_4:41295402-41295421 Msa0500740:intron 30.0%
TCCCAATTTATTTGACATCT+TGG - chr3_4:41295406-41295425 None:intergenic 30.0%
AGATGTCAAATAAATTGGGA+CGG + chr3_4:41295406-41295425 Msa0500740:intron 30.0%
GAAAGATGAAATGGTGAATG+TGG - chr3_4:41294430-41294449 None:intergenic 35.0%
! AAGGTTTGTTCTTTCTTCTG+TGG + chr3_4:41294534-41294553 Msa0500740:intron 35.0%
!!! TGTTTTAGGAAACGGTGAAA+AGG - chr3_4:41294889-41294908 None:intergenic 35.0%
! GTGAGTCACAATAGTGTTTT+AGG - chr3_4:41294903-41294922 None:intergenic 35.0%
ACCAACTTGCACATAAATGA+TGG + chr3_4:41294924-41294943 Msa0500740:intron 35.0%
TCCATCATTTATGTGCAAGT+TGG - chr3_4:41294928-41294947 None:intergenic 35.0%
GAAAATGGGTCAGTTATTCT+GGG - chr3_4:41295218-41295237 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+TGG - chr3_4:41295219-41295238 None:intergenic 35.0%
GAAAGATGAAATGGTGAATG+TGG - chr3_4:41294430-41294449 None:intergenic 35.0%
! AAGGTTTGTTCTTTCTTCTG+TGG + chr3_4:41294534-41294553 Msa0500740:intron 35.0%
!!! TGTTTTAGGAAACGGTGAAA+AGG - chr3_4:41294889-41294908 None:intergenic 35.0%
! GTGAGTCACAATAGTGTTTT+AGG - chr3_4:41294903-41294922 None:intergenic 35.0%
ACCAACTTGCACATAAATGA+TGG + chr3_4:41294924-41294943 Msa0500740:intron 35.0%
TCCATCATTTATGTGCAAGT+TGG - chr3_4:41294928-41294947 None:intergenic 35.0%
GAAAATGGGTCAGTTATTCT+GGG - chr3_4:41295218-41295237 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+TGG - chr3_4:41295219-41295238 None:intergenic 35.0%
!!! AAGGTGAGGGTTTTGAAATG+GGG - chr3_4:41294297-41294316 None:intergenic 40.0%
!!! GAAGGTGAGGGTTTTGAAAT+GGG - chr3_4:41294298-41294317 None:intergenic 40.0%
AACGAGCTCTTTCTTGATAG+GGG - chr3_4:41294332-41294351 None:intergenic 40.0%
CAACGAGCTCTTTCTTGATA+GGG - chr3_4:41294333-41294352 None:intergenic 40.0%
ACAACGAGCTCTTTCTTGAT+AGG - chr3_4:41294334-41294353 None:intergenic 40.0%
ACTTCTTTGTAAACAGGAGC+AGG - chr3_4:41294388-41294407 None:intergenic 40.0%
! GAAATGGTGAATGTGGGTTA+GGG - chr3_4:41294423-41294442 None:intergenic 40.0%
TGAAATGGTGAATGTGGGTT+AGG - chr3_4:41294424-41294443 None:intergenic 40.0%
TGTCAAATAAATTGGGACGG+AGG + chr3_4:41295409-41295428 Msa0500740:intron 40.0%
GTCAAATAAATTGGGACGGA+GGG + chr3_4:41295410-41295429 Msa0500740:intron 40.0%
!!! AAGGTGAGGGTTTTGAAATG+GGG - chr3_4:41294297-41294316 None:intergenic 40.0%
!!! GAAGGTGAGGGTTTTGAAAT+GGG - chr3_4:41294298-41294317 None:intergenic 40.0%
AACGAGCTCTTTCTTGATAG+GGG - chr3_4:41294332-41294351 None:intergenic 40.0%
CAACGAGCTCTTTCTTGATA+GGG - chr3_4:41294333-41294352 None:intergenic 40.0%
ACAACGAGCTCTTTCTTGAT+AGG - chr3_4:41294334-41294353 None:intergenic 40.0%
ACTTCTTTGTAAACAGGAGC+AGG - chr3_4:41294388-41294407 None:intergenic 40.0%
! GAAATGGTGAATGTGGGTTA+GGG - chr3_4:41294423-41294442 None:intergenic 40.0%
TGAAATGGTGAATGTGGGTT+AGG - chr3_4:41294424-41294443 None:intergenic 40.0%
TGTCAAATAAATTGGGACGG+AGG + chr3_4:41295409-41295428 Msa0500740:intron 40.0%
GTCAAATAAATTGGGACGGA+GGG + chr3_4:41295410-41295429 Msa0500740:intron 40.0%
ACTACACAGAGCTGAGAACT+TGG + chr3_4:41294126-41294145 Msa0500740:five_prime_UTR 45.0%
GTGAAGTGATTGAGAGAGCT+TGG - chr3_4:41294263-41294282 None:intergenic 45.0%
!!! AGGTGAGGGTTTTGAAATGG+GGG - chr3_4:41294296-41294315 None:intergenic 45.0%
!!! GGAAGGTGAGGGTTTTGAAA+TGG - chr3_4:41294299-41294318 None:intergenic 45.0%
! CTCGTTGTTGTTCGAAGATC+CGG + chr3_4:41294346-41294365 Msa0500740:CDS 45.0%
GTAAACAGGAGCAGGCATAT+TGG - chr3_4:41294380-41294399 None:intergenic 45.0%
! ATGGGTCAGTTATTCTGGGA+CGG - chr3_4:41295214-41295233 None:intergenic 45.0%
ACTACACAGAGCTGAGAACT+TGG + chr3_4:41294126-41294145 Msa0500740:five_prime_UTR 45.0%
GTGAAGTGATTGAGAGAGCT+TGG - chr3_4:41294263-41294282 None:intergenic 45.0%
!!! AGGTGAGGGTTTTGAAATGG+GGG - chr3_4:41294296-41294315 None:intergenic 45.0%
!!! GGAAGGTGAGGGTTTTGAAA+TGG - chr3_4:41294299-41294318 None:intergenic 45.0%
! CTCGTTGTTGTTCGAAGATC+CGG + chr3_4:41294346-41294365 Msa0500740:CDS 45.0%
GTAAACAGGAGCAGGCATAT+TGG - chr3_4:41294380-41294399 None:intergenic 45.0%
! ATGGGTCAGTTATTCTGGGA+CGG - chr3_4:41295214-41295233 None:intergenic 45.0%
!!! TATTTAAAAGTTTATATTTT+TGG - chr3_4:41295045-41295064 None:intergenic 5.0%
!!! TATTTAAAAGTTTATATTTT+TGG - chr3_4:41295045-41295064 None:intergenic 5.0%
CATCATACGAAGAGAGTCGC+CGG + chr3_4:41294191-41294210 Msa0500740:CDS 50.0%
AGAGAGTCGCCGGAAAAGAA+TGG + chr3_4:41294201-41294220 Msa0500740:CDS 50.0%
! TCTTTCTTGATAGGGGTGCG+AGG - chr3_4:41294325-41294344 None:intergenic 50.0%
!! GGTGAATGTGGGTTAGGGTT+TGG - chr3_4:41294418-41294437 None:intergenic 50.0%
!! GTCAGTTATTCTGGGACGGA+GGG - chr3_4:41295210-41295229 None:intergenic 50.0%
CATCATACGAAGAGAGTCGC+CGG + chr3_4:41294191-41294210 Msa0500740:CDS 50.0%
AGAGAGTCGCCGGAAAAGAA+TGG + chr3_4:41294201-41294220 Msa0500740:CDS 50.0%
! TCTTTCTTGATAGGGGTGCG+AGG - chr3_4:41294325-41294344 None:intergenic 50.0%
!! GGTGAATGTGGGTTAGGGTT+TGG - chr3_4:41294418-41294437 None:intergenic 50.0%
!! GTCAGTTATTCTGGGACGGA+GGG - chr3_4:41295210-41295229 None:intergenic 50.0%
GTGCGAGGTTTGGAAGGTGA+GGG - chr3_4:41294310-41294329 None:intergenic 55.0%
! ATAGGGGTGCGAGGTTTGGA+AGG - chr3_4:41294316-41294335 None:intergenic 55.0%
! CTTGATAGGGGTGCGAGGTT+TGG - chr3_4:41294320-41294339 None:intergenic 55.0%
AGGCATATTGGCGACACGAC+CGG - chr3_4:41294368-41294387 None:intergenic 55.0%
!! GGTCAGTTATTCTGGGACGG+AGG - chr3_4:41295211-41295230 None:intergenic 55.0%
GTGCGAGGTTTGGAAGGTGA+GGG - chr3_4:41294310-41294329 None:intergenic 55.0%
! ATAGGGGTGCGAGGTTTGGA+AGG - chr3_4:41294316-41294335 None:intergenic 55.0%
! CTTGATAGGGGTGCGAGGTT+TGG - chr3_4:41294320-41294339 None:intergenic 55.0%
AGGCATATTGGCGACACGAC+CGG - chr3_4:41294368-41294387 None:intergenic 55.0%
!! GGTCAGTTATTCTGGGACGG+AGG - chr3_4:41295211-41295230 None:intergenic 55.0%
GGTGCGAGGTTTGGAAGGTG+AGG - chr3_4:41294311-41294330 None:intergenic 60.0%
GGTGCGAGGTTTGGAAGGTG+AGG - chr3_4:41294311-41294330 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 41294068 41295539 41294068 ID=Msa0500740;Name=Msa0500740
chr3_4 mRNA 41294068 41295539 41294068 ID=Msa0500740-mRNA-1;Parent=Msa0500740;Name=Msa0500740-mRNA-1;_AED=0.02;_eAED=0.02;_QI=107|0.5|0.33|0.66|1|1|3|0|94
chr3_4 exon 41294068 41294405 41294068 ID=Msa0500740-mRNA-1:exon:8586;Parent=Msa0500740-mRNA-1
chr3_4 exon 41294493 41294536 41294493 ID=Msa0500740-mRNA-1:exon:8587;Parent=Msa0500740-mRNA-1
chr3_4 exon 41295530 41295539 41295530 ID=Msa0500740-mRNA-1:exon:8588;Parent=Msa0500740-mRNA-1
chr3_4 five_prime_UTR 41294068 41294174 41294068 ID=Msa0500740-mRNA-1:five_prime_utr;Parent=Msa0500740-mRNA-1
chr3_4 CDS 41294175 41294405 41294175 ID=Msa0500740-mRNA-1:cds;Parent=Msa0500740-mRNA-1
chr3_4 CDS 41294493 41294536 41294493 ID=Msa0500740-mRNA-1:cds;Parent=Msa0500740-mRNA-1
chr3_4 CDS 41295530 41295539 41295530 ID=Msa0500740-mRNA-1:cds;Parent=Msa0500740-mRNA-1
Gene Sequence

>Msa0500740

ATGGTGGCAAAAGCATCATCATACGAAGAGAGTCGCCGGAAAAGAATGGAAGAAAATCAAAAGAGAATGGAAGCTCTCAATCTTACCAAGCTCTCTCAATCACTTCACAAATCATCTTCCCCCATTTCAAAACCCTCACCTTCCAAACCTCGCACCCCTATCAAGAAAGAGCTCGTTGTTGTTCGAAGATCCGGTCGTGTCGCCAATATGCCTGCTCCTGTTTACAAAGAAATTCTTATTGATTCCGTTAAAATACCTAGAAATAGAATTTCAAGGATGTGCTAG

Protein sequence

>Msa0500740

MVAKASSYEESRRKRMEENQKRMEALNLTKLSQSLHKSSSPISKPSPSKPRTPIKKELVVVRRSGRVANMPAPVYKEILIDSVKIPRNRISRMC*