AlfalfaGEDB | Msa0559870

M. sativa cultivar zhongmu-4 / Msa0559870

KEGG: -
GO: -
PFAM: PF14705.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0559870	XP_003608396.1	98.824	85	1	0	1	85	1	85	3.46e-54	172

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0559870	sp\|Q6AVK1\|COSA_ORYSJ	81.176	85	16	0	1	85	1	85	4.42e-45	142

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0559870	G7JV45	98.824	85	1	0	1	85	1	85	1.65e-54	172

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa0559870	0.803845	2.870557e-49	-8.615850e-47
Msa0081520	Msa0559870	0.828451	8.927273e-55	-8.615850e-47
Msa0093850	Msa0559870	0.818174	2.248908e-52	-8.615850e-47
Msa0138360	Msa0559870	0.805477	1.310035e-49	-8.615850e-47
Msa0147980	Msa0559870	0.807916	4.000627e-50	-8.615850e-47
Msa0173890	Msa0559870	0.825055	5.775491e-54	-8.615850e-47
Msa0423750	Msa0559870	0.801530	8.623050e-49	-8.615850e-47
Msa0470530	Msa0559870	0.806253	8.998265e-50	-8.615850e-47
Msa0249540	Msa0559870	0.802589	5.222665e-49	-8.615850e-47
Msa0277450	Msa0559870	0.800286	1.547568e-48	-8.615850e-47
Msa0315980	Msa0559870	0.836526	8.887449e-57	-8.615850e-47
Msa0326590	Msa0559870	0.816611	5.059214e-52	-8.615850e-47
Msa0341690	Msa0559870	0.826667	2.392065e-54	-8.615850e-47
Msa0527020	Msa0559870	0.802002	6.898998e-49	-8.615850e-47
Msa0527190	Msa0559870	0.809794	1.586331e-50	-8.615850e-47
Msa0559870	Msa0607520	0.806199	9.238959e-50	-8.615850e-47
Msa0559870	Msa0614000	0.827364	1.629756e-54	-8.615850e-47
Msa0559870	Msa0626450	0.825821	3.802775e-54	-8.615850e-47
Msa0559870	Msa0626490	0.826542	2.561892e-54	-8.615850e-47
Msa0559870	Msa0638480	0.873906	1.022669e-67	-8.615850e-47
Msa0559870	Msa0647820	0.803203	3.899510e-49	-8.615850e-47
Msa0559870	Msa0651550	0.801880	7.306013e-49	-8.615850e-47
Msa0559870	Msa0664600	0.818771	1.647011e-52	-8.615850e-47
Msa0559870	Msa0669520	0.811981	5.332999e-51	-8.615850e-47
Msa0559870	Msa0690540	0.816027	6.834500e-52	-8.615850e-47
Msa0559870	Msa0697160	0.854187	1.454798e-61	-8.615850e-47
Msa0559870	Msa0738040	0.802445	5.592334e-49	-8.615850e-47
Msa0559870	Msa0749230	0.820676	6.048784e-53	-8.615850e-47
Msa0559870	Msa0765240	0.806983	6.311414e-50	-8.615850e-47
Msa0559870	Msa0808800	0.820176	7.878039e-53	-8.615850e-47
Msa0559870	Msa0943440	0.802448	5.582794e-49	-8.615850e-47
Msa0559870	Msa0980140	0.804350	2.252806e-49	-8.615850e-47
Msa0559870	Msa1007820	-0.801106	1.052747e-48	-8.615850e-47
Msa0559870	Msa1055010	0.811150	8.085371e-51	-8.615850e-47
Msa0559870	Msa1079230	0.803567	3.277232e-49	-8.615850e-47
Msa0559870	Msa1124110	0.821779	3.367243e-53	-8.615850e-47
Msa0559870	Msa1127590	0.814900	1.217189e-51	-8.615850e-47
Msa0559870	Msa1140210	0.800265	1.562811e-48	-8.615850e-47
Msa0559870	Msa1168950	0.810648	1.038500e-50	-8.615850e-47
Msa0559870	Msa1169040	0.810726	9.985226e-51	-8.615850e-47
Msa0559870	Msa1356830	0.801431	9.032280e-49	-8.615850e-47
Msa0559870	Msa1369150	0.804275	2.335947e-49	-8.615850e-47
Msa0559870	Msa1386710	0.804090	2.551877e-49	-8.615850e-47
Msa0559870	Msa1429930	0.816268	6.036173e-52	-8.615850e-47
Msa0559870	Msa1437940	0.821246	4.471696e-53	-8.615850e-47
Msa0559870	Msa1440960	0.816270	6.030205e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0559870	MtrunA17_Chr4g0049791	98.824	85	1	0	1	85	1	85	3.18e-58	172
Msa0559870	MtrunA17_Chr2g0279651	85.882	85	12	0	1	85	1	85	7.55e-51	154

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0559870	AT4G33640.1	80.769	78	15	0	1	78	1	78	1.13e-41	132
Msa0559870	AT4G33640.2	80.769	78	15	0	1	78	67	144	3.49e-41	132

Find 33 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTTGATTCACAAACTAAA+TGG	0.258730	4_1:-59046233	None:intergenic
TGCATTTACTAAAACTGATT+TGG	0.274717	4_1:+59046404	Msa0559870:three_prime_UTR
TGCATTGTGAAGGTCACATT+TGG	0.398089	4_1:+59045897	Msa0559870:intron
AGTTGATGCTTAAATTTCAA+TGG	0.407398	4_1:-59046360	None:intergenic
CCTGCTGCTGCTAACTGATT+CGG	0.442731	4_1:+59046071	Msa0559870:exon
GAGTGATTTCCACATTATCA+TGG	0.446001	4_1:-59046037	None:intergenic
GTGTGTTTAAAGTTTCTGTT+CGG	0.456320	4_1:-59045070	None:intergenic
GAACACTCTTTCACGATGAT+CGG	0.491735	4_1:+59045919	Msa0559870:CDS
GTTAGTGATTCAAGCGGTAA+AGG	0.493166	4_1:-59046115	None:intergenic
AAGAGGAGGTTGAAAAGCTG+AGG	0.496528	4_1:+59045155	Msa0559870:CDS
CCGAATCAGTTAGCAGCAGC+AGG	0.502169	4_1:-59046071	None:intergenic
CAGTTAGCAGCAGCAGGAGC+AGG	0.504867	4_1:-59046065	None:intergenic
CTTGGAAATGTTCAAGCAGA+TGG	0.516658	4_1:+59045193	Msa0559870:CDS
CACTTCGTAAATAATAGTAT+AGG	0.525059	4_1:-59046328	None:intergenic
CTTAAATTTCAATGGTTCTG+TGG	0.531142	4_1:-59046352	None:intergenic
GCTAACTGATTCGGTTGTGA+AGG	0.539593	4_1:+59046080	Msa0559870:exon
AGGGAAGAAATAAAGAGGCT+TGG	0.542290	4_1:+59045175	Msa0559870:CDS
TGTGAAGATGAACGTAGAAG+AGG	0.563453	4_1:+59045138	Msa0559870:exon
GAAGATGAACGTAGAAGAGG+AGG	0.565552	4_1:+59045141	Msa0559870:exon
AGCTGAGGGAAGAAATAAAG+AGG	0.569879	4_1:+59045170	Msa0559870:CDS
AGAGGAGGTTGAAAAGCTGA+GGG	0.572997	4_1:+59045156	Msa0559870:CDS
ATAGTATAGGATAAACATGA+AGG	0.579841	4_1:-59046315	None:intergenic
CTATACTATTATTTACGAAG+TGG	0.588267	4_1:+59046329	Msa0559870:three_prime_UTR
AATATATTTGAAGCACTCGT+CGG	0.601303	4_1:+59045948	Msa0559870:CDS
TCAAGCAGATGGTTCTTGCA+AGG	0.604584	4_1:+59045204	Msa0559870:CDS
CTAACTGATTCGGTTGTGAA+GGG	0.617075	4_1:+59046081	Msa0559870:exon
GCAAAATCTACATATTTGAG+TGG	0.628837	4_1:+59046255	Msa0559870:three_prime_UTR
GACGGTGAACTACTGTTGCA+AGG	0.632747	4_1:+59046011	Msa0559870:CDS
AAACAGGTTAGTGATTCAAG+CGG	0.665757	4_1:-59046121	None:intergenic
GATGTTGTAATATATGCATG+TGG	0.675462	4_1:+59046173	Msa0559870:three_prime_UTR
GCAAGGAGTCCATGATAATG+TGG	0.676630	4_1:+59046028	Msa0559870:CDS
TGTAATATATGCATGTGGAG+AGG	0.745183	4_1:+59046178	Msa0559870:three_prime_UTR
CGTAAAGTAGTTGCATACGA+CGG	0.749247	4_1:+59045993	Msa0559870:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATAAATATAAAAAGCTTTT+AGG	+	chr4_1:59045047-59045066	Msa0559870:five_prime_UTR	10.0%
!!!	AATAAATATAAAAAGCTTTT+AGG	+	chr4_1:59045047-59045066	Msa0559870:five_prime_UTR	10.0%
!!!	AATTTGTTTATTATAAACCA+TGG	+	chr4_1:59045523-59045542	Msa0559870:intron	15.0%
!!	ATAATTGTAATTAAATGTGT+TGG	+	chr4_1:59045683-59045702	Msa0559870:intron	15.0%
!!!	AATTTGTTTATTATAAACCA+TGG	+	chr4_1:59045523-59045542	Msa0559870:intron	15.0%
!!	ATAATTGTAATTAAATGTGT+TGG	+	chr4_1:59045683-59045702	Msa0559870:intron	15.0%
!!	GAATCAAGTTCTTAATTAAA+AGG	-	chr4_1:59045310-59045329	None:intergenic	20.0%
!!	TTTCATATGATATTTCGTAA+TGG	-	chr4_1:59045443-59045462	None:intergenic	20.0%
!!	TAATATATAGGTAGATACAT+TGG	-	chr4_1:59045504-59045523	None:intergenic	20.0%
!!!	AAGTATTGATATGATGTTTA+GGG	-	chr4_1:59045648-59045667	None:intergenic	20.0%
!!!	TAAGTATTGATATGATGTTT+AGG	-	chr4_1:59045649-59045668	None:intergenic	20.0%
!!	GAATCAAGTTCTTAATTAAA+AGG	-	chr4_1:59045310-59045329	None:intergenic	20.0%
!!	TTTCATATGATATTTCGTAA+TGG	-	chr4_1:59045443-59045462	None:intergenic	20.0%
!!	TAATATATAGGTAGATACAT+TGG	-	chr4_1:59045504-59045523	None:intergenic	20.0%
!!!	AAGTATTGATATGATGTTTA+GGG	-	chr4_1:59045648-59045667	None:intergenic	20.0%
!!!	TAAGTATTGATATGATGTTT+AGG	-	chr4_1:59045649-59045668	None:intergenic	20.0%
!!!	AAGAAAGACTTTCTTGTTTT+TGG	+	chr4_1:59045096-59045115	Msa0559870:five_prime_UTR	25.0%
!!	GAAAATGTATATGGTTTGTA+AGG	-	chr4_1:59045263-59045282	None:intergenic	25.0%
!	ATGCATCATGAAAATGTATA+TGG	-	chr4_1:59045272-59045291	None:intergenic	25.0%
!	TTCAGATCTAGATATATTGA+TGG	+	chr4_1:59045327-59045346	Msa0559870:intron	25.0%
!	TCAGATCTAGATATATTGAT+GGG	+	chr4_1:59045328-59045347	Msa0559870:intron	25.0%
!	TTGTTTATTATAAACCATGG+AGG	+	chr4_1:59045526-59045545	Msa0559870:intron	25.0%
!!!	CATATCAGTTTTATATGGAA+TGG	-	chr4_1:59045571-59045590	None:intergenic	25.0%
!	CATTCCATATAAAACTGATA+TGG	+	chr4_1:59045569-59045588	Msa0559870:intron	25.0%
!!!	AATTTGCTGAAATAGTACTA+TGG	+	chr4_1:59045743-59045762	Msa0559870:intron	25.0%
!	AAATATGTAATGCATTGTGA+AGG	+	chr4_1:59045887-59045906	Msa0559870:intron	25.0%
!	CATTTGATTCACAAACTAAA+TGG	-	chr4_1:59046236-59046255	None:intergenic	25.0%
!	ATAGTATAGGATAAACATGA+AGG	-	chr4_1:59046318-59046337	None:intergenic	25.0%
!	CACTTCGTAAATAATAGTAT+AGG	-	chr4_1:59046331-59046350	None:intergenic	25.0%
!	CTATACTATTATTTACGAAG+TGG	+	chr4_1:59046329-59046348	Msa0559870:three_prime_UTR	25.0%
!!	AGTTGATGCTTAAATTTCAA+TGG	-	chr4_1:59046363-59046382	None:intergenic	25.0%
!	TGCATTTACTAAAACTGATT+TGG	+	chr4_1:59046404-59046423	Msa0559870:three_prime_UTR	25.0%
!!!	AAGAAAGACTTTCTTGTTTT+TGG	+	chr4_1:59045096-59045115	Msa0559870:five_prime_UTR	25.0%
!!	GAAAATGTATATGGTTTGTA+AGG	-	chr4_1:59045263-59045282	None:intergenic	25.0%
!	ATGCATCATGAAAATGTATA+TGG	-	chr4_1:59045272-59045291	None:intergenic	25.0%
!	TTCAGATCTAGATATATTGA+TGG	+	chr4_1:59045327-59045346	Msa0559870:intron	25.0%
!	TCAGATCTAGATATATTGAT+GGG	+	chr4_1:59045328-59045347	Msa0559870:intron	25.0%
!	TTGTTTATTATAAACCATGG+AGG	+	chr4_1:59045526-59045545	Msa0559870:intron	25.0%
!!!	CATATCAGTTTTATATGGAA+TGG	-	chr4_1:59045571-59045590	None:intergenic	25.0%
!	CATTCCATATAAAACTGATA+TGG	+	chr4_1:59045569-59045588	Msa0559870:intron	25.0%
!!!	AATTTGCTGAAATAGTACTA+TGG	+	chr4_1:59045743-59045762	Msa0559870:intron	25.0%
!	AAATATGTAATGCATTGTGA+AGG	+	chr4_1:59045887-59045906	Msa0559870:intron	25.0%
!	CATTTGATTCACAAACTAAA+TGG	-	chr4_1:59046236-59046255	None:intergenic	25.0%
!	ATAGTATAGGATAAACATGA+AGG	-	chr4_1:59046318-59046337	None:intergenic	25.0%
!	CACTTCGTAAATAATAGTAT+AGG	-	chr4_1:59046331-59046350	None:intergenic	25.0%
!	CTATACTATTATTTACGAAG+TGG	+	chr4_1:59046329-59046348	Msa0559870:three_prime_UTR	25.0%
!!	AGTTGATGCTTAAATTTCAA+TGG	-	chr4_1:59046363-59046382	None:intergenic	25.0%
!	TGCATTTACTAAAACTGATT+TGG	+	chr4_1:59046404-59046423	Msa0559870:three_prime_UTR	25.0%
!	GTGTGTTTAAAGTTTCTGTT+CGG	-	chr4_1:59045073-59045092	None:intergenic	30.0%
!!!	AAAGACTTTCTTGTTTTTGG+TGG	+	chr4_1:59045099-59045118	Msa0559870:five_prime_UTR	30.0%
	TTCTTGCAAGGTATAACAAA+AGG	+	chr4_1:59045216-59045235	Msa0559870:intron	30.0%
!	TGATGGGTATTTATTGAATG+TGG	+	chr4_1:59045344-59045363	Msa0559870:intron	30.0%
!	TGGTCACTTTCTTATTCTTA+TGG	+	chr4_1:59045364-59045383	Msa0559870:intron	30.0%
!!	CTAGCCATATCAGTTTTATA+TGG	-	chr4_1:59045576-59045595	None:intergenic	30.0%
!	CTATATCTTCAGTTGATTCA+TGG	-	chr4_1:59045778-59045797	None:intergenic	30.0%
	TATAGTGAGTGATGGTTAAT+TGG	+	chr4_1:59045793-59045812	Msa0559870:intron	30.0%
!	AATATATTTGAAGCACTCGT+CGG	+	chr4_1:59045948-59045967	Msa0559870:CDS	30.0%
!!!	GAATCACTAACCTGTTTTTA+TGG	+	chr4_1:59046127-59046146	Msa0559870:three_prime_UTR	30.0%
	GATGTTGTAATATATGCATG+TGG	+	chr4_1:59046173-59046192	Msa0559870:three_prime_UTR	30.0%
	GCAAAATCTACATATTTGAG+TGG	+	chr4_1:59046255-59046274	Msa0559870:three_prime_UTR	30.0%
!	CTTAAATTTCAATGGTTCTG+TGG	-	chr4_1:59046355-59046374	None:intergenic	30.0%
!	GTGTGTTTAAAGTTTCTGTT+CGG	-	chr4_1:59045073-59045092	None:intergenic	30.0%
!!!	AAAGACTTTCTTGTTTTTGG+TGG	+	chr4_1:59045099-59045118	Msa0559870:five_prime_UTR	30.0%
	TTCTTGCAAGGTATAACAAA+AGG	+	chr4_1:59045216-59045235	Msa0559870:intron	30.0%
!	TGATGGGTATTTATTGAATG+TGG	+	chr4_1:59045344-59045363	Msa0559870:intron	30.0%
!	TGGTCACTTTCTTATTCTTA+TGG	+	chr4_1:59045364-59045383	Msa0559870:intron	30.0%
!!	CTAGCCATATCAGTTTTATA+TGG	-	chr4_1:59045576-59045595	None:intergenic	30.0%
!	CTATATCTTCAGTTGATTCA+TGG	-	chr4_1:59045778-59045797	None:intergenic	30.0%
	TATAGTGAGTGATGGTTAAT+TGG	+	chr4_1:59045793-59045812	Msa0559870:intron	30.0%
!	AATATATTTGAAGCACTCGT+CGG	+	chr4_1:59045948-59045967	Msa0559870:CDS	30.0%
!!!	GAATCACTAACCTGTTTTTA+TGG	+	chr4_1:59046127-59046146	Msa0559870:three_prime_UTR	30.0%
	GATGTTGTAATATATGCATG+TGG	+	chr4_1:59046173-59046192	Msa0559870:three_prime_UTR	30.0%
	GCAAAATCTACATATTTGAG+TGG	+	chr4_1:59046255-59046274	Msa0559870:three_prime_UTR	30.0%
!	CTTAAATTTCAATGGTTCTG+TGG	-	chr4_1:59046355-59046374	None:intergenic	30.0%
	TCTTATGGATCTAGATCACA+AGG	+	chr4_1:59045379-59045398	Msa0559870:intron	35.0%
	ACTGAAGATATAGTGAGTGA+TGG	+	chr4_1:59045785-59045804	Msa0559870:intron	35.0%
	GTTAATGCGCATATGTGAAT+TGG	+	chr4_1:59045823-59045842	Msa0559870:intron	35.0%
	GAGTGATTTCCACATTATCA+TGG	-	chr4_1:59046040-59046059	None:intergenic	35.0%
	AAACAGGTTAGTGATTCAAG+CGG	-	chr4_1:59046124-59046143	None:intergenic	35.0%
	TGTAATATATGCATGTGGAG+AGG	+	chr4_1:59046178-59046197	Msa0559870:three_prime_UTR	35.0%
	TCTTATGGATCTAGATCACA+AGG	+	chr4_1:59045379-59045398	Msa0559870:intron	35.0%
	ACTGAAGATATAGTGAGTGA+TGG	+	chr4_1:59045785-59045804	Msa0559870:intron	35.0%
	GTTAATGCGCATATGTGAAT+TGG	+	chr4_1:59045823-59045842	Msa0559870:intron	35.0%
	GAGTGATTTCCACATTATCA+TGG	-	chr4_1:59046040-59046059	None:intergenic	35.0%
	AAACAGGTTAGTGATTCAAG+CGG	-	chr4_1:59046124-59046143	None:intergenic	35.0%
	TGTAATATATGCATGTGGAG+AGG	+	chr4_1:59046178-59046197	Msa0559870:three_prime_UTR	35.0%
	TGTGAAGATGAACGTAGAAG+AGG	+	chr4_1:59045138-59045157	Msa0559870:exon	40.0%
	AGCTGAGGGAAGAAATAAAG+AGG	+	chr4_1:59045170-59045189	Msa0559870:CDS	40.0%
	AGGGAAGAAATAAAGAGGCT+TGG	+	chr4_1:59045175-59045194	Msa0559870:CDS	40.0%
	CTTGGAAATGTTCAAGCAGA+TGG	+	chr4_1:59045193-59045212	Msa0559870:CDS	40.0%
	CACAAGGTCATTCACAACAT+TGG	+	chr4_1:59045395-59045414	Msa0559870:intron	40.0%
	TGCATTGTGAAGGTCACATT+TGG	+	chr4_1:59045897-59045916	Msa0559870:intron	40.0%
	GAACACTCTTTCACGATGAT+CGG	+	chr4_1:59045919-59045938	Msa0559870:CDS	40.0%
	CGTAAAGTAGTTGCATACGA+CGG	+	chr4_1:59045993-59046012	Msa0559870:CDS	40.0%
!!	CTAACTGATTCGGTTGTGAA+GGG	+	chr4_1:59046081-59046100	Msa0559870:exon	40.0%
	GTTAGTGATTCAAGCGGTAA+AGG	-	chr4_1:59046118-59046137	None:intergenic	40.0%
!!!	CACTAACCTGTTTTTATGGC+TGG	+	chr4_1:59046131-59046150	Msa0559870:three_prime_UTR	40.0%
	ATCACACCAGCCATAAAAAC+AGG	-	chr4_1:59046140-59046159	None:intergenic	40.0%
	TGTGAAGATGAACGTAGAAG+AGG	+	chr4_1:59045138-59045157	Msa0559870:exon	40.0%
	AGCTGAGGGAAGAAATAAAG+AGG	+	chr4_1:59045170-59045189	Msa0559870:CDS	40.0%
	AGGGAAGAAATAAAGAGGCT+TGG	+	chr4_1:59045175-59045194	Msa0559870:CDS	40.0%
	CTTGGAAATGTTCAAGCAGA+TGG	+	chr4_1:59045193-59045212	Msa0559870:CDS	40.0%
	CACAAGGTCATTCACAACAT+TGG	+	chr4_1:59045395-59045414	Msa0559870:intron	40.0%
	TGCATTGTGAAGGTCACATT+TGG	+	chr4_1:59045897-59045916	Msa0559870:intron	40.0%
	GAACACTCTTTCACGATGAT+CGG	+	chr4_1:59045919-59045938	Msa0559870:CDS	40.0%
	CGTAAAGTAGTTGCATACGA+CGG	+	chr4_1:59045993-59046012	Msa0559870:CDS	40.0%
!!	CTAACTGATTCGGTTGTGAA+GGG	+	chr4_1:59046081-59046100	Msa0559870:exon	40.0%
	GTTAGTGATTCAAGCGGTAA+AGG	-	chr4_1:59046118-59046137	None:intergenic	40.0%
!!!	CACTAACCTGTTTTTATGGC+TGG	+	chr4_1:59046131-59046150	Msa0559870:three_prime_UTR	40.0%
	ATCACACCAGCCATAAAAAC+AGG	-	chr4_1:59046140-59046159	None:intergenic	40.0%
	GAAGATGAACGTAGAAGAGG+AGG	+	chr4_1:59045141-59045160	Msa0559870:exon	45.0%
	AAGAGGAGGTTGAAAAGCTG+AGG	+	chr4_1:59045155-59045174	Msa0559870:CDS	45.0%
	AGAGGAGGTTGAAAAGCTGA+GGG	+	chr4_1:59045156-59045175	Msa0559870:CDS	45.0%
	TCAAGCAGATGGTTCTTGCA+AGG	+	chr4_1:59045204-59045223	Msa0559870:CDS	45.0%
!	GCAAGGAGTCCATGATAATG+TGG	+	chr4_1:59046028-59046047	Msa0559870:CDS	45.0%
!!	GCTAACTGATTCGGTTGTGA+AGG	+	chr4_1:59046080-59046099	Msa0559870:exon	45.0%
	GAAGATGAACGTAGAAGAGG+AGG	+	chr4_1:59045141-59045160	Msa0559870:exon	45.0%
	AAGAGGAGGTTGAAAAGCTG+AGG	+	chr4_1:59045155-59045174	Msa0559870:CDS	45.0%
	AGAGGAGGTTGAAAAGCTGA+GGG	+	chr4_1:59045156-59045175	Msa0559870:CDS	45.0%
	TCAAGCAGATGGTTCTTGCA+AGG	+	chr4_1:59045204-59045223	Msa0559870:CDS	45.0%
!	GCAAGGAGTCCATGATAATG+TGG	+	chr4_1:59046028-59046047	Msa0559870:CDS	45.0%
!!	GCTAACTGATTCGGTTGTGA+AGG	+	chr4_1:59046080-59046099	Msa0559870:exon	45.0%
!!	TAATAAACAAATTAATATAT+AGG	-	chr4_1:59045516-59045535	None:intergenic	5.0%
!!	TAATAAACAAATTAATATAT+AGG	-	chr4_1:59045516-59045535	None:intergenic	5.0%
	CGCATGATATCTCTCCTCCA+TGG	-	chr4_1:59045543-59045562	None:intergenic	50.0%
	GACGGTGAACTACTGTTGCA+AGG	+	chr4_1:59046011-59046030	Msa0559870:CDS	50.0%
	CCTGCTGCTGCTAACTGATT+CGG	+	chr4_1:59046071-59046090	Msa0559870:exon	50.0%
	CGCATGATATCTCTCCTCCA+TGG	-	chr4_1:59045543-59045562	None:intergenic	50.0%
	GACGGTGAACTACTGTTGCA+AGG	+	chr4_1:59046011-59046030	Msa0559870:CDS	50.0%
	CCTGCTGCTGCTAACTGATT+CGG	+	chr4_1:59046071-59046090	Msa0559870:exon	50.0%
	CCGAATCAGTTAGCAGCAGC+AGG	-	chr4_1:59046074-59046093	None:intergenic	55.0%
	CCGAATCAGTTAGCAGCAGC+AGG	-	chr4_1:59046074-59046093	None:intergenic	55.0%
	CAGTTAGCAGCAGCAGGAGC+AGG	-	chr4_1:59046068-59046087	None:intergenic	60.0%
	CAGTTAGCAGCAGCAGGAGC+AGG	-	chr4_1:59046068-59046087	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	59045022	59046443	59045022	ID=Msa0559870;Name=Msa0559870
chr4_1	mRNA	59045022	59046443	59045022	ID=Msa0559870-mRNA-1;Parent=Msa0559870;Name=Msa0559870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=123\|1\|1\|1\|1\|1\|2\|355\|86
chr4_1	exon	59045022	59045225	59045022	ID=Msa0559870-mRNA-1:exon:18143;Parent=Msa0559870-mRNA-1
chr4_1	exon	59045909	59046443	59045909	ID=Msa0559870-mRNA-1:exon:18144;Parent=Msa0559870-mRNA-1
chr4_1	five_prime_UTR	59045022	59045144	59045022	ID=Msa0559870-mRNA-1:five_prime_utr;Parent=Msa0559870-mRNA-1
chr4_1	CDS	59045145	59045225	59045145	ID=Msa0559870-mRNA-1:cds;Parent=Msa0559870-mRNA-1
chr4_1	CDS	59045909	59046088	59045909	ID=Msa0559870-mRNA-1:cds;Parent=Msa0559870-mRNA-1
chr4_1	three_prime_UTR	59046089	59046443	59046089	ID=Msa0559870-mRNA-1:three_prime_utr;Parent=Msa0559870-mRNA-1

Gene Sequence	>Msa0559870 ATGAACGTAGAAGAGGAGGTTGAAAAGCTGAGGGAAGAAATAAAGAGGCTTGGAAATGTTCAAGCAGATGGTTCTTGCAAGGTCACATTTGGAACACTCTTTCACGATGATCGGTGCGCAAATATATTTGAAGCACTCGTCGGTACACTAAGAGCTGCTAAAAAGCGTAAAGTAGTTGCATACGACGGTGAACTACTGTTGCAAGGAGTCCATGATAATGTGGAAATCACTCTTAATCCTGCTCCTGCTGCTGCTAACTGA
Protein sequence	>Msa0559870 MNVEEEVEKLREEIKRLGNVQADGSCKVTFGTLFHDDRCANIFEALVGTLRAAKKRKVVAYDGELLLQGVHDNVEITLNPAPAAAN*