AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1055010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1055010 MtrunA17_Chr4g0072411 100.000 103 0 0 1 103 59 161 5.39e-69 203
Msa1055010 MtrunA17_Chr5g0425951 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr4g0030431 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr3g0100121 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr3g0113681 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr2g0327641 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr3g0103351 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr8g0352791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr3g0103821 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr3g0104031 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr7g0225731 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr3g0103801 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr3g0103881 100.000 103 0 0 1 103 1 103 2.26e-68 199
Msa1055010 MtrunA17_Chr7g0262791 100.000 103 0 0 1 103 1 103 2.26e-68 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1055010 AT3G53730.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT1G07820.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT1G07820.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT5G59690.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT3G46320.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT2G28740.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT1G07660.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT3G45930.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT5G59970.1 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT5G59970.2 100.000 103 0 0 1 103 1 103 3.11e-68 199
Msa1055010 AT1G07660.2 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 33 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCTTCCCTGTCTCTTA+AGG 0.300277 7_2:-28133850 None:intergenic
GGAAAGGGAGGTAAGGGATT+AGG 0.384736 7_2:+28133593 Msa1055010:CDS
AGGAGTGTTGAAGATCTTCT+TGG 0.386764 7_2:+28133748 Msa1055010:CDS
TCGCTGGCTTTGTGATTCCT+TGG 0.395127 7_2:-28133661 None:intergenic
GGTTCTTCCCTGTCTCTTAA+GGG 0.396932 7_2:-28133849 None:intergenic
AATCAGCGGTTTGATTTATG+AGG 0.401936 7_2:+28133718 Msa1055010:CDS
AGACAGGGAAGAACCCTCTA+TGG 0.440459 7_2:+28133857 Msa1055010:CDS
GGAAGAACCCTCTATGGTTT+CGG 0.453170 7_2:+28133863 Msa1055010:CDS
TGTTTATGCCCTTAAGAGAC+AGG 0.454713 7_2:+28133841 Msa1055010:CDS
AGTCTTCCTCCTCGCATGCT+CGG 0.461551 7_2:-28133801 None:intergenic
GGTAAGGGATTAGGAAAGGG+AGG 0.470949 7_2:+28133602 Msa1055010:CDS
CTCGCCAATCGACGAATCGC+TGG 0.484651 7_2:-28133677 None:intergenic
TGGTCGTGGAAAGGGAGGTA+AGG 0.490380 7_2:+28133586 Msa1055010:CDS
TTCAACCACCGAAACCATAG+AGG 0.495992 7_2:-28133871 None:intergenic
AAAATGTCTGGTCGTGGAAA+GGG 0.502849 7_2:+28133578 None:intergenic
AAAGCCAGCGATTCGTCGAT+TGG 0.503407 7_2:+28133673 Msa1055010:CDS
GGAGGTAAGGGATTAGGAAA+GGG 0.512610 7_2:+28133599 Msa1055010:CDS
CGTCGATTGGCGAGAAGAGG+AGG 0.518547 7_2:+28133686 Msa1055010:CDS
GGGAGGTAAGGGATTAGGAA+AGG 0.522188 7_2:+28133598 Msa1055010:CDS
GAGGAAGACTGTGACTGCTA+TGG 0.530595 7_2:+28133814 Msa1055010:CDS
GGTCGTGGAAAGGGAGGTAA+GGG 0.572418 7_2:+28133587 Msa1055010:CDS
TTGATTTATGAGGAAACAAG+AGG 0.572644 7_2:+28133728 Msa1055010:CDS
ATTCGTCGATTGGCGAGAAG+AGG 0.594887 7_2:+28133683 Msa1055010:CDS
AGAACCCTCTATGGTTTCGG+TGG 0.607847 7_2:+28133866 Msa1055010:CDS
TTACTTACACCGAGCATGCG+AGG 0.610843 7_2:+28133792 Msa1055010:CDS
GTTCTCCGCGACAACATCCA+AGG 0.619358 7_2:+28133644 Msa1055010:CDS
TGATTCCTTGGATGTTGTCG+CGG 0.639365 7_2:-28133649 None:intergenic
ATGTCTGGTCGTGGAAAGGG+AGG 0.640958 7_2:+28133581 Msa1055010:CDS
GTTTATGCCCTTAAGAGACA+GGG 0.645697 7_2:+28133842 Msa1055010:CDS
AGGTGCAAAGAGACATCGCA+AGG 0.665418 7_2:+28133622 Msa1055010:CDS
TCAACCACCGAAACCATAGA+GGG 0.678652 7_2:-28133870 None:intergenic
GGAGGTGTGAAAAGAATCAG+CGG 0.710972 7_2:+28133704 Msa1055010:CDS
CTTACACCGAGCATGCGAGG+AGG 0.753233 7_2:+28133795 Msa1055010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGATTTATGAGGAAACAAG+AGG + chr7_2:28133728-28133747 Msa1055010:CDS 30.0%
! AATCAGCGGTTTGATTTATG+AGG + chr7_2:28133718-28133737 Msa1055010:CDS 35.0%
!! AGGAGTGTTGAAGATCTTCT+TGG + chr7_2:28133748-28133767 Msa1055010:CDS 40.0%
TGTTTATGCCCTTAAGAGAC+AGG + chr7_2:28133841-28133860 Msa1055010:CDS 40.0%
GTTTATGCCCTTAAGAGACA+GGG + chr7_2:28133842-28133861 Msa1055010:CDS 40.0%
GGAGGTAAGGGATTAGGAAA+GGG + chr7_2:28133599-28133618 Msa1055010:CDS 45.0%
TGATTCCTTGGATGTTGTCG+CGG - chr7_2:28133652-28133671 None:intergenic 45.0%
! GGAGGTGTGAAAAGAATCAG+CGG + chr7_2:28133704-28133723 Msa1055010:CDS 45.0%
GGTTCTTCCCTGTCTCTTAA+GGG - chr7_2:28133852-28133871 None:intergenic 45.0%
GGAAGAACCCTCTATGGTTT+CGG + chr7_2:28133863-28133882 Msa1055010:CDS 45.0%
TCAACCACCGAAACCATAGA+GGG - chr7_2:28133873-28133892 None:intergenic 45.0%
GGAAAGGGAGGTAAGGGATT+AGG + chr7_2:28133593-28133612 Msa1055010:CDS 50.0%
GGGAGGTAAGGGATTAGGAA+AGG + chr7_2:28133598-28133617 Msa1055010:CDS 50.0%
GGTAAGGGATTAGGAAAGGG+AGG + chr7_2:28133602-28133621 Msa1055010:CDS 50.0%
AGGTGCAAAGAGACATCGCA+AGG + chr7_2:28133622-28133641 Msa1055010:CDS 50.0%
!! TCGCTGGCTTTGTGATTCCT+TGG - chr7_2:28133664-28133683 None:intergenic 50.0%
AAAGCCAGCGATTCGTCGAT+TGG + chr7_2:28133673-28133692 Msa1055010:CDS 50.0%
! ATTCGTCGATTGGCGAGAAG+AGG + chr7_2:28133683-28133702 Msa1055010:CDS 50.0%
TTACTTACACCGAGCATGCG+AGG + chr7_2:28133792-28133811 Msa1055010:CDS 50.0%
GAGGAAGACTGTGACTGCTA+TGG + chr7_2:28133814-28133833 Msa1055010:CDS 50.0%
GGGTTCTTCCCTGTCTCTTA+AGG - chr7_2:28133853-28133872 None:intergenic 50.0%
AGACAGGGAAGAACCCTCTA+TGG + chr7_2:28133857-28133876 Msa1055010:CDS 50.0%
AGAACCCTCTATGGTTTCGG+TGG + chr7_2:28133866-28133885 Msa1055010:CDS 50.0%
ATGTCTGGTCGTGGAAAGGG+AGG + chr7_2:28133581-28133600 Msa1055010:CDS 55.0%
TGGTCGTGGAAAGGGAGGTA+AGG + chr7_2:28133586-28133605 Msa1055010:CDS 55.0%
GGTCGTGGAAAGGGAGGTAA+GGG + chr7_2:28133587-28133606 Msa1055010:CDS 55.0%
GTTCTCCGCGACAACATCCA+AGG + chr7_2:28133644-28133663 Msa1055010:CDS 55.0%
AGTCTTCCTCCTCGCATGCT+CGG - chr7_2:28133804-28133823 None:intergenic 55.0%
CTCGCCAATCGACGAATCGC+TGG - chr7_2:28133680-28133699 None:intergenic 60.0%
! CGTCGATTGGCGAGAAGAGG+AGG + chr7_2:28133686-28133705 Msa1055010:CDS 60.0%
CTTACACCGAGCATGCGAGG+AGG + chr7_2:28133795-28133814 Msa1055010:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 28133581 28133892 28133581 ID=Msa1055010;Name=Msa1055010
chr7_2 mRNA 28133581 28133892 28133581 ID=Msa1055010-mRNA-1;Parent=Msa1055010;Name=Msa1055010-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|103
chr7_2 exon 28133581 28133892 28133581 ID=Msa1055010-mRNA-1:exon:5900;Parent=Msa1055010-mRNA-1
chr7_2 CDS 28133581 28133892 28133581 ID=Msa1055010-mRNA-1:cds;Parent=Msa1055010-mRNA-1
Gene Sequence

>Msa1055010

ATGTCTGGTCGTGGAAAGGGAGGTAAGGGATTAGGAAAGGGAGGTGCAAAGAGACATCGCAAGGTTCTCCGCGACAACATCCAAGGAATCACAAAGCCAGCGATTCGTCGATTGGCGAGAAGAGGAGGTGTGAAAAGAATCAGCGGTTTGATTTATGAGGAAACAAGAGGAGTGTTGAAGATCTTCTTGGAGAATGTGATAAGAGATGCTGTTACTTACACCGAGCATGCGAGGAGGAAGACTGTGACTGCTATGGATGTTGTTTATGCCCTTAAGAGACAGGGAAGAACCCTCTATGGTTTCGGTGGTTGA

Protein sequence

>Msa1055010

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*