AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0614000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614000 MtrunA17_Chr4g0049791 98.824 85 1 0 1 85 1 85 3.18e-58 172
Msa0614000 MtrunA17_Chr2g0279651 85.882 85 12 0 1 85 1 85 7.55e-51 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614000 AT4G33640.1 80.769 78 15 0 1 78 1 78 1.13e-41 132
Msa0614000 AT4G33640.2 80.769 78 15 0 1 78 67 144 3.49e-41 132

Find 32 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGATTCACAAACTAAA+TGG 0.258730 4_2:-62924629 None:intergenic
TGCATTTACTAAAACAGATT+TGG 0.294396 4_2:+62924799 Msa0614000:three_prime_UTR
TGCATTGTGAAGGTCACATT+TGG 0.398089 4_2:+62924293 Msa0614000:intron
AGTTGATGCTTAAATTTCAA+TGG 0.407398 4_2:-62924755 None:intergenic
CCTGCTGCTGCTAACTGATT+CGG 0.442731 4_2:+62924467 Msa0614000:exon
GAGTGATTTCCACATTATCA+TGG 0.446001 4_2:-62924433 None:intergenic
GTGTGTTTAAAGTTTCTGTT+CGG 0.456320 4_2:-62923464 None:intergenic
CCACTTCGTAAATATAGTAT+AGG 0.477828 4_2:-62924724 None:intergenic
GAACACTCTTTCACGATGAT+CGG 0.491735 4_2:+62924315 Msa0614000:CDS
AAGAGGAGGTTGAAAAGCTG+AGG 0.496528 4_2:+62923549 Msa0614000:CDS
CCGAATCAGTTAGCAGCAGC+AGG 0.502169 4_2:-62924467 None:intergenic
CAGTTAGCAGCAGCAGGAGC+AGG 0.504867 4_2:-62924461 None:intergenic
GTTAGTGATTCAAGCAGTAA+AGG 0.512252 4_2:-62924511 None:intergenic
CTTGGAAATGTTCAAGCAGA+TGG 0.516658 4_2:+62923587 Msa0614000:CDS
AGGGAAGAAATCAAGAGGCT+TGG 0.527446 4_2:+62923569 Msa0614000:CDS
CTTAAATTTCAATGGTTCTG+TGG 0.531142 4_2:-62924747 None:intergenic
GCTAACTGATTCGGTTGTGA+AGG 0.539593 4_2:+62924476 Msa0614000:exon
TGTGAAGATGAACGTAGAAG+AGG 0.563453 4_2:+62923532 Msa0614000:exon
GAAGATGAACGTAGAAGAGG+AGG 0.565552 4_2:+62923535 Msa0614000:exon
AGAGGAGGTTGAAAAGCTGA+GGG 0.572997 4_2:+62923550 Msa0614000:CDS
ATAGTATAGGATAAACATGA+AGG 0.579841 4_2:-62924711 None:intergenic
CCTATACTATATTTACGAAG+TGG 0.591359 4_2:+62924724 Msa0614000:three_prime_UTR
AATATATTTGAAGCACTCGT+CGG 0.601303 4_2:+62924344 Msa0614000:CDS
TCAAGCAGATGGTTCTTGCA+AGG 0.604584 4_2:+62923598 Msa0614000:CDS
CTAACTGATTCGGTTGTGAA+GGG 0.617075 4_2:+62924477 Msa0614000:exon
AGCTGAGGGAAGAAATCAAG+AGG 0.626324 4_2:+62923564 Msa0614000:CDS
GCAAAATCTACATATTTGAG+TGG 0.628837 4_2:+62924651 Msa0614000:three_prime_UTR
GACGGTGAACTACTGTTGCA+AGG 0.632747 4_2:+62924407 Msa0614000:CDS
GATGTTGTAATATATGCATG+TGG 0.675462 4_2:+62924569 Msa0614000:three_prime_UTR
GCAAGGAGTCCATGATAATG+TGG 0.676630 4_2:+62924424 Msa0614000:CDS
TGTAATATATGCATGTGGAG+AGG 0.735312 4_2:+62924574 Msa0614000:three_prime_UTR
CGTAAAGTAGTTGCATACGA+CGG 0.749247 4_2:+62924389 Msa0614000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAATATAAAAAGCTTTT+AGG + chr4_2:62923441-62923460 Msa0614000:five_prime_UTR 10.0%
!! TAATAAACAAATTAATGTAT+AGG - chr4_2:62923910-62923929 None:intergenic 10.0%
!!! AATAAATATAAAAAGCTTTT+AGG + chr4_2:62923441-62923460 Msa0614000:five_prime_UTR 10.0%
!! TAATAAACAAATTAATGTAT+AGG - chr4_2:62923910-62923929 None:intergenic 10.0%
!!! AATTTGTTTATTATAAACCA+TGG + chr4_2:62923917-62923936 Msa0614000:intron 15.0%
!! ATAATTGTAATTAAATGTGT+TGG + chr4_2:62924079-62924098 Msa0614000:intron 15.0%
!!! AATTTGTTTATTATAAACCA+TGG + chr4_2:62923917-62923936 Msa0614000:intron 15.0%
!! ATAATTGTAATTAAATGTGT+TGG + chr4_2:62924079-62924098 Msa0614000:intron 15.0%
!! GAATCAAGTTCTTAATTAAA+AGG - chr4_2:62923704-62923723 None:intergenic 20.0%
!! TTTCATATGATATTTCGTAA+TGG - chr4_2:62923837-62923856 None:intergenic 20.0%
!!! AAGTATTGATATGATGTTTA+GGG - chr4_2:62924044-62924063 None:intergenic 20.0%
!!! TAAGTATTGATATGATGTTT+AGG - chr4_2:62924045-62924064 None:intergenic 20.0%
!! GAATCAAGTTCTTAATTAAA+AGG - chr4_2:62923704-62923723 None:intergenic 20.0%
!! TTTCATATGATATTTCGTAA+TGG - chr4_2:62923837-62923856 None:intergenic 20.0%
!!! AAGTATTGATATGATGTTTA+GGG - chr4_2:62924044-62924063 None:intergenic 20.0%
!!! TAAGTATTGATATGATGTTT+AGG - chr4_2:62924045-62924064 None:intergenic 20.0%
!!! AAGAAAGACTTTCTTGTTTT+TGG + chr4_2:62923490-62923509 Msa0614000:five_prime_UTR 25.0%
!! GAAAATGTATATGGTTTGTA+AGG - chr4_2:62923657-62923676 None:intergenic 25.0%
! ATGCATCATGAAAATGTATA+TGG - chr4_2:62923666-62923685 None:intergenic 25.0%
! TTCAGATCTAGATATATTGA+TGG + chr4_2:62923721-62923740 Msa0614000:intron 25.0%
! TCAGATCTAGATATATTGAT+GGG + chr4_2:62923722-62923741 Msa0614000:intron 25.0%
! TAATGTATAGGTAGATACAT+TGG - chr4_2:62923898-62923917 None:intergenic 25.0%
! TTGTTTATTATAAACCATGG+AGG + chr4_2:62923920-62923939 Msa0614000:intron 25.0%
!!! CATATCAGTTTTATATGGAA+TGG - chr4_2:62923965-62923984 None:intergenic 25.0%
! CATTCCATATAAAACTGATA+TGG + chr4_2:62923963-62923982 Msa0614000:intron 25.0%
!!! AATTTGCTGAAATAGTACTA+TGG + chr4_2:62924139-62924158 Msa0614000:intron 25.0%
! AAATATGTAATGCATTGTGA+AGG + chr4_2:62924283-62924302 Msa0614000:intron 25.0%
! CATTTGATTCACAAACTAAA+TGG - chr4_2:62924632-62924651 None:intergenic 25.0%
! ATAGTATAGGATAAACATGA+AGG - chr4_2:62924714-62924733 None:intergenic 25.0%
!! AGTTGATGCTTAAATTTCAA+TGG - chr4_2:62924758-62924777 None:intergenic 25.0%
! TGCATTTACTAAAACAGATT+TGG + chr4_2:62924799-62924818 Msa0614000:three_prime_UTR 25.0%
!!! AAGAAAGACTTTCTTGTTTT+TGG + chr4_2:62923490-62923509 Msa0614000:five_prime_UTR 25.0%
!! GAAAATGTATATGGTTTGTA+AGG - chr4_2:62923657-62923676 None:intergenic 25.0%
! ATGCATCATGAAAATGTATA+TGG - chr4_2:62923666-62923685 None:intergenic 25.0%
! TTCAGATCTAGATATATTGA+TGG + chr4_2:62923721-62923740 Msa0614000:intron 25.0%
! TCAGATCTAGATATATTGAT+GGG + chr4_2:62923722-62923741 Msa0614000:intron 25.0%
! TAATGTATAGGTAGATACAT+TGG - chr4_2:62923898-62923917 None:intergenic 25.0%
! TTGTTTATTATAAACCATGG+AGG + chr4_2:62923920-62923939 Msa0614000:intron 25.0%
!!! CATATCAGTTTTATATGGAA+TGG - chr4_2:62923965-62923984 None:intergenic 25.0%
! CATTCCATATAAAACTGATA+TGG + chr4_2:62923963-62923982 Msa0614000:intron 25.0%
!!! AATTTGCTGAAATAGTACTA+TGG + chr4_2:62924139-62924158 Msa0614000:intron 25.0%
! AAATATGTAATGCATTGTGA+AGG + chr4_2:62924283-62924302 Msa0614000:intron 25.0%
! CATTTGATTCACAAACTAAA+TGG - chr4_2:62924632-62924651 None:intergenic 25.0%
! ATAGTATAGGATAAACATGA+AGG - chr4_2:62924714-62924733 None:intergenic 25.0%
!! AGTTGATGCTTAAATTTCAA+TGG - chr4_2:62924758-62924777 None:intergenic 25.0%
! TGCATTTACTAAAACAGATT+TGG + chr4_2:62924799-62924818 Msa0614000:three_prime_UTR 25.0%
! GTGTGTTTAAAGTTTCTGTT+CGG - chr4_2:62923467-62923486 None:intergenic 30.0%
!!! AAAGACTTTCTTGTTTTTGG+TGG + chr4_2:62923493-62923512 Msa0614000:five_prime_UTR 30.0%
TTCTTGCAAGGTATAACAAA+AGG + chr4_2:62923610-62923629 Msa0614000:intron 30.0%
! TGATGGGTATTTATTGAATG+TGG + chr4_2:62923738-62923757 Msa0614000:intron 30.0%
! TGGTCACTTTCTTATTCTTA+TGG + chr4_2:62923758-62923777 Msa0614000:intron 30.0%
!! CTAGCCATATCAGTTTTATA+TGG - chr4_2:62923970-62923989 None:intergenic 30.0%
! CTATATCTTCAGTTGATTCA+TGG - chr4_2:62924174-62924193 None:intergenic 30.0%
TATAGTGAGTGATGGTTAAT+TGG + chr4_2:62924189-62924208 Msa0614000:intron 30.0%
! AATATATTTGAAGCACTCGT+CGG + chr4_2:62924344-62924363 Msa0614000:CDS 30.0%
!!! GAATCACTAACCTGTTTTTA+TGG + chr4_2:62924523-62924542 Msa0614000:three_prime_UTR 30.0%
GATGTTGTAATATATGCATG+TGG + chr4_2:62924569-62924588 Msa0614000:three_prime_UTR 30.0%
GCAAAATCTACATATTTGAG+TGG + chr4_2:62924651-62924670 Msa0614000:three_prime_UTR 30.0%
CCACTTCGTAAATATAGTAT+AGG - chr4_2:62924727-62924746 None:intergenic 30.0%
CCTATACTATATTTACGAAG+TGG + chr4_2:62924724-62924743 Msa0614000:three_prime_UTR 30.0%
! CTTAAATTTCAATGGTTCTG+TGG - chr4_2:62924750-62924769 None:intergenic 30.0%
! GTGTGTTTAAAGTTTCTGTT+CGG - chr4_2:62923467-62923486 None:intergenic 30.0%
!!! AAAGACTTTCTTGTTTTTGG+TGG + chr4_2:62923493-62923512 Msa0614000:five_prime_UTR 30.0%
TTCTTGCAAGGTATAACAAA+AGG + chr4_2:62923610-62923629 Msa0614000:intron 30.0%
! TGATGGGTATTTATTGAATG+TGG + chr4_2:62923738-62923757 Msa0614000:intron 30.0%
! TGGTCACTTTCTTATTCTTA+TGG + chr4_2:62923758-62923777 Msa0614000:intron 30.0%
!! CTAGCCATATCAGTTTTATA+TGG - chr4_2:62923970-62923989 None:intergenic 30.0%
! CTATATCTTCAGTTGATTCA+TGG - chr4_2:62924174-62924193 None:intergenic 30.0%
TATAGTGAGTGATGGTTAAT+TGG + chr4_2:62924189-62924208 Msa0614000:intron 30.0%
! AATATATTTGAAGCACTCGT+CGG + chr4_2:62924344-62924363 Msa0614000:CDS 30.0%
!!! GAATCACTAACCTGTTTTTA+TGG + chr4_2:62924523-62924542 Msa0614000:three_prime_UTR 30.0%
GATGTTGTAATATATGCATG+TGG + chr4_2:62924569-62924588 Msa0614000:three_prime_UTR 30.0%
GCAAAATCTACATATTTGAG+TGG + chr4_2:62924651-62924670 Msa0614000:three_prime_UTR 30.0%
CCACTTCGTAAATATAGTAT+AGG - chr4_2:62924727-62924746 None:intergenic 30.0%
CCTATACTATATTTACGAAG+TGG + chr4_2:62924724-62924743 Msa0614000:three_prime_UTR 30.0%
! CTTAAATTTCAATGGTTCTG+TGG - chr4_2:62924750-62924769 None:intergenic 30.0%
TCTTATGGATCTAGATCACA+AGG + chr4_2:62923773-62923792 Msa0614000:intron 35.0%
ACTGAAGATATAGTGAGTGA+TGG + chr4_2:62924181-62924200 Msa0614000:intron 35.0%
GTTAATGCGCATATGTGAAT+TGG + chr4_2:62924219-62924238 Msa0614000:intron 35.0%
GAGTGATTTCCACATTATCA+TGG - chr4_2:62924436-62924455 None:intergenic 35.0%
GTTAGTGATTCAAGCAGTAA+AGG - chr4_2:62924514-62924533 None:intergenic 35.0%
TGTAATATATGCATGTGGAG+AGG + chr4_2:62924574-62924593 Msa0614000:three_prime_UTR 35.0%
TCTTATGGATCTAGATCACA+AGG + chr4_2:62923773-62923792 Msa0614000:intron 35.0%
ACTGAAGATATAGTGAGTGA+TGG + chr4_2:62924181-62924200 Msa0614000:intron 35.0%
GTTAATGCGCATATGTGAAT+TGG + chr4_2:62924219-62924238 Msa0614000:intron 35.0%
GAGTGATTTCCACATTATCA+TGG - chr4_2:62924436-62924455 None:intergenic 35.0%
GTTAGTGATTCAAGCAGTAA+AGG - chr4_2:62924514-62924533 None:intergenic 35.0%
TGTAATATATGCATGTGGAG+AGG + chr4_2:62924574-62924593 Msa0614000:three_prime_UTR 35.0%
TGTGAAGATGAACGTAGAAG+AGG + chr4_2:62923532-62923551 Msa0614000:exon 40.0%
CTTGGAAATGTTCAAGCAGA+TGG + chr4_2:62923587-62923606 Msa0614000:CDS 40.0%
CACAAGGTCATTCACAACAT+TGG + chr4_2:62923789-62923808 Msa0614000:intron 40.0%
TGCATTGTGAAGGTCACATT+TGG + chr4_2:62924293-62924312 Msa0614000:intron 40.0%
GAACACTCTTTCACGATGAT+CGG + chr4_2:62924315-62924334 Msa0614000:CDS 40.0%
CGTAAAGTAGTTGCATACGA+CGG + chr4_2:62924389-62924408 Msa0614000:CDS 40.0%
!! CTAACTGATTCGGTTGTGAA+GGG + chr4_2:62924477-62924496 Msa0614000:exon 40.0%
!!! CACTAACCTGTTTTTATGGC+TGG + chr4_2:62924527-62924546 Msa0614000:three_prime_UTR 40.0%
ATCACACCAGCCATAAAAAC+AGG - chr4_2:62924536-62924555 None:intergenic 40.0%
TGTGAAGATGAACGTAGAAG+AGG + chr4_2:62923532-62923551 Msa0614000:exon 40.0%
CTTGGAAATGTTCAAGCAGA+TGG + chr4_2:62923587-62923606 Msa0614000:CDS 40.0%
CACAAGGTCATTCACAACAT+TGG + chr4_2:62923789-62923808 Msa0614000:intron 40.0%
TGCATTGTGAAGGTCACATT+TGG + chr4_2:62924293-62924312 Msa0614000:intron 40.0%
GAACACTCTTTCACGATGAT+CGG + chr4_2:62924315-62924334 Msa0614000:CDS 40.0%
CGTAAAGTAGTTGCATACGA+CGG + chr4_2:62924389-62924408 Msa0614000:CDS 40.0%
!! CTAACTGATTCGGTTGTGAA+GGG + chr4_2:62924477-62924496 Msa0614000:exon 40.0%
!!! CACTAACCTGTTTTTATGGC+TGG + chr4_2:62924527-62924546 Msa0614000:three_prime_UTR 40.0%
ATCACACCAGCCATAAAAAC+AGG - chr4_2:62924536-62924555 None:intergenic 40.0%
GAAGATGAACGTAGAAGAGG+AGG + chr4_2:62923535-62923554 Msa0614000:exon 45.0%
AAGAGGAGGTTGAAAAGCTG+AGG + chr4_2:62923549-62923568 Msa0614000:CDS 45.0%
AGAGGAGGTTGAAAAGCTGA+GGG + chr4_2:62923550-62923569 Msa0614000:CDS 45.0%
AGCTGAGGGAAGAAATCAAG+AGG + chr4_2:62923564-62923583 Msa0614000:CDS 45.0%
AGGGAAGAAATCAAGAGGCT+TGG + chr4_2:62923569-62923588 Msa0614000:CDS 45.0%
TCAAGCAGATGGTTCTTGCA+AGG + chr4_2:62923598-62923617 Msa0614000:CDS 45.0%
! GCAAGGAGTCCATGATAATG+TGG + chr4_2:62924424-62924443 Msa0614000:CDS 45.0%
!! GCTAACTGATTCGGTTGTGA+AGG + chr4_2:62924476-62924495 Msa0614000:exon 45.0%
GAAGATGAACGTAGAAGAGG+AGG + chr4_2:62923535-62923554 Msa0614000:exon 45.0%
AAGAGGAGGTTGAAAAGCTG+AGG + chr4_2:62923549-62923568 Msa0614000:CDS 45.0%
AGAGGAGGTTGAAAAGCTGA+GGG + chr4_2:62923550-62923569 Msa0614000:CDS 45.0%
AGCTGAGGGAAGAAATCAAG+AGG + chr4_2:62923564-62923583 Msa0614000:CDS 45.0%
AGGGAAGAAATCAAGAGGCT+TGG + chr4_2:62923569-62923588 Msa0614000:CDS 45.0%
TCAAGCAGATGGTTCTTGCA+AGG + chr4_2:62923598-62923617 Msa0614000:CDS 45.0%
! GCAAGGAGTCCATGATAATG+TGG + chr4_2:62924424-62924443 Msa0614000:CDS 45.0%
!! GCTAACTGATTCGGTTGTGA+AGG + chr4_2:62924476-62924495 Msa0614000:exon 45.0%
CGCATGATATCTCTCCTCCA+TGG - chr4_2:62923937-62923956 None:intergenic 50.0%
GACGGTGAACTACTGTTGCA+AGG + chr4_2:62924407-62924426 Msa0614000:CDS 50.0%
CCTGCTGCTGCTAACTGATT+CGG + chr4_2:62924467-62924486 Msa0614000:exon 50.0%
CGCATGATATCTCTCCTCCA+TGG - chr4_2:62923937-62923956 None:intergenic 50.0%
GACGGTGAACTACTGTTGCA+AGG + chr4_2:62924407-62924426 Msa0614000:CDS 50.0%
CCTGCTGCTGCTAACTGATT+CGG + chr4_2:62924467-62924486 Msa0614000:exon 50.0%
CCGAATCAGTTAGCAGCAGC+AGG - chr4_2:62924470-62924489 None:intergenic 55.0%
CCGAATCAGTTAGCAGCAGC+AGG - chr4_2:62924470-62924489 None:intergenic 55.0%
CAGTTAGCAGCAGCAGGAGC+AGG - chr4_2:62924464-62924483 None:intergenic 60.0%
CAGTTAGCAGCAGCAGGAGC+AGG - chr4_2:62924464-62924483 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 62923416 62924838 62923416 ID=Msa0614000;Name=Msa0614000
chr4_2 mRNA 62923416 62924838 62923416 ID=Msa0614000-mRNA-1;Parent=Msa0614000;Name=Msa0614000-mRNA-1;_AED=0.00;_eAED=0.00;_QI=123|1|1|1|1|1|2|354|86
chr4_2 exon 62923416 62923619 62923416 ID=Msa0614000-mRNA-1:exon:17419;Parent=Msa0614000-mRNA-1
chr4_2 exon 62924305 62924838 62924305 ID=Msa0614000-mRNA-1:exon:17420;Parent=Msa0614000-mRNA-1
chr4_2 five_prime_UTR 62923416 62923538 62923416 ID=Msa0614000-mRNA-1:five_prime_utr;Parent=Msa0614000-mRNA-1
chr4_2 CDS 62923539 62923619 62923539 ID=Msa0614000-mRNA-1:cds;Parent=Msa0614000-mRNA-1
chr4_2 CDS 62924305 62924484 62924305 ID=Msa0614000-mRNA-1:cds;Parent=Msa0614000-mRNA-1
chr4_2 three_prime_UTR 62924485 62924838 62924485 ID=Msa0614000-mRNA-1:three_prime_utr;Parent=Msa0614000-mRNA-1
Gene Sequence

>Msa0614000

ATGAACGTAGAAGAGGAGGTTGAAAAGCTGAGGGAAGAAATCAAGAGGCTTGGAAATGTTCAAGCAGATGGTTCTTGCAAGGTCACATTTGGAACACTCTTTCACGATGATCGGTGCGCAAATATATTTGAAGCACTCGTCGGTACACTAAGAGCTGCTAAAAAGCGTAAAGTAGTTGCATACGACGGTGAACTACTGTTGCAAGGAGTCCATGATAATGTGGAAATCACTCTTAATCCTGCTCCTGCTGCTGCTAACTGA

Protein sequence

>Msa0614000

MNVEEEVEKLREEIKRLGNVQADGSCKVTFGTLFHDDRCANIFEALVGTLRAAKKRKVVAYDGELLLQGVHDNVEITLNPAPAAAN*