AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0671170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0671170 MtrunA17_Chr4g0010271 100.000 219 0 0 1 219 1 219 5.99e-166 456
Msa0671170 MtrunA17_Chr1g0169861 78.995 219 46 0 1 219 51 269 2.50e-138 388
Msa0671170 MtrunA17_Chr7g0237561 84.018 219 35 0 1 219 1 219 1.72e-129 363
Msa0671170 MtrunA17_Chr2g0296021 71.233 219 63 0 1 219 1 219 1.10e-122 346
Msa0671170 MtrunA17_Chr4g0043521 79.275 193 40 0 3 195 5 197 3.93e-121 342
Msa0671170 MtrunA17_Chr2g0291651 74.419 215 55 0 2 216 6 220 5.40e-115 327
Msa0671170 MtrunA17_Chr7g0237521 75.385 195 48 0 1 195 1 195 1.39e-111 318
Msa0671170 MtrunA17_Chr1g0210521 56.303 238 85 1 1 219 1 238 7.10e-102 294
Msa0671170 MtrunA17_Chr4g0010211 95.370 108 5 0 64 171 3 110 1.90e-71 213
Msa0671170 MtrunA17_Chr1g0169851 69.178 146 30 1 57 187 8 153 4.04e-70 211
Msa0671170 MtrunA17_Chr5g0441741 39.151 212 126 3 6 216 3 212 1.50e-49 160
Msa0671170 MtrunA17_Chr4g0026121 36.279 215 134 3 5 218 2 214 4.33e-46 152
Msa0671170 MtrunA17_Chr4g0064371 33.180 217 136 5 6 216 3 216 6.54e-41 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0671170 AT1G04760.2 80.365 219 43 0 1 219 1 219 2.25e-133 374
Msa0671170 AT1G04760.1 80.365 219 43 0 1 219 1 219 2.25e-133 374
Msa0671170 AT1G04750.1 83.105 219 37 0 1 219 1 219 3.29e-129 363
Msa0671170 AT2G32670.1 82.192 219 39 0 1 219 66 284 5.30e-129 365
Msa0671170 AT2G33120.1 83.105 219 37 0 1 219 1 219 1.24e-128 362
Msa0671170 AT2G33120.2 79.736 227 38 1 1 219 1 227 2.07e-125 354
Msa0671170 AT3G54300.3 56.723 238 84 2 1 219 1 238 8.02e-101 292
Msa0671170 AT3G54300.2 56.723 238 84 2 1 219 1 238 8.02e-101 292
Msa0671170 AT3G54300.1 56.723 238 84 2 1 219 1 238 8.02e-101 292
Msa0671170 AT2G33110.1 67.580 219 67 1 1 219 1 215 5.90e-97 281
Msa0671170 AT4G15780.1 70.391 179 52 1 1 178 1 179 2.49e-95 276
Msa0671170 AT2G33110.2 69.753 162 49 0 1 162 1 162 3.45e-85 251
Msa0671170 AT1G04750.2 84.397 141 22 0 79 219 1 141 3.06e-74 221
Msa0671170 AT1G04750.4 84.397 141 22 0 79 219 1 141 3.06e-74 221
Msa0671170 AT1G04750.3 84.397 141 22 0 79 219 1 141 3.06e-74 221
Msa0671170 AT5G11150.1 40.553 217 123 5 5 218 2 215 7.85e-53 169
Msa0671170 AT5G11150.2 40.553 217 123 5 5 218 2 215 1.45e-52 169
Msa0671170 AT5G22360.1 37.963 216 130 3 5 218 2 215 7.00e-51 164
Msa0671170 AT4G32150.1 38.498 213 128 3 5 216 2 212 2.37e-48 158
Msa0671170 AT2G25340.1 37.850 214 128 4 5 216 2 212 1.59e-43 145
Msa0671170 AT2G33120.3 88.372 86 10 0 134 219 52 137 3.87e-36 124
Msa0671170 AT3G24890.2 53.191 94 38 2 113 200 14 107 5.20e-25 95.5
Msa0671170 AT3G24890.1 53.125 64 26 1 113 176 14 73 2.18e-15 68.9
Msa0671170 AT3G24890.4 53.125 64 26 1 113 176 14 73 2.18e-15 68.9
Msa0671170 AT3G24890.3 53.125 64 26 1 113 176 14 73 2.18e-15 68.9
Msa0671170 AT3G24890.5 53.125 64 26 1 113 176 14 73 2.18e-15 68.9

Find 95 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTGTATTGTTTCATTTA+TGG 0.065324 4_4:+14162546 Msa0671170:three_prime_UTR
AAAAGTCTTAACAAAGAATT+TGG 0.112791 4_4:+14160184 Msa0671170:CDS
CTTTGCAGGCACAAGATTTC+AGG 0.218031 4_4:+14162010 Msa0671170:intron
GAGAGAGGTGGTGTCAAATT+TGG 0.233821 4_4:-14158727 None:intergenic
AGAATTGTCACTACATATAT+TGG 0.236659 4_4:+14162180 Msa0671170:three_prime_UTR
TTCAGCTTCCTCGTCGATAA+TGG 0.292725 4_4:+14159054 Msa0671170:CDS
ACCACATCTTTCTCCTCAAC+TGG 0.295387 4_4:-14162044 None:intergenic
GTTATTTGCAGGTTATTGAT+AGG 0.304636 4_4:+14161435 Msa0671170:intron
GTTAGGAAGGGAAGTGAATA+AGG 0.319681 4_4:+14162289 Msa0671170:three_prime_UTR
TGCATTTGCTCCTTCAACTT+TGG 0.326828 4_4:-14160293 None:intergenic
ATACGTTCAAGAAAAGCTAT+TGG 0.353729 4_4:-14160106 None:intergenic
CTCCGGTCACCACCAGGGTC+AGG 0.379723 4_4:-14162492 None:intergenic
TACCTCTGTAAGCGCCCTTT+CGG 0.391076 4_4:+14162456 Msa0671170:three_prime_UTR
ATCATTGATCTATAGCTTTG+TGG 0.397799 4_4:+14158903 Msa0671170:CDS
AACTCCCTGCTTCTAACAAC+AGG 0.399581 4_4:+14159004 Msa0671170:CDS
TTTGTTTATTAGACATTCAA+TGG 0.402021 4_4:+14162250 Msa0671170:three_prime_UTR
AACTGAGAACCTTCGCTCTC+AGG 0.417900 4_4:+14161487 Msa0671170:CDS
GCCCTGACCCTGGTGGTGAC+CGG 0.421928 4_4:+14162490 Msa0671170:three_prime_UTR
GCAATATTGTGTGGAACATC+CGG 0.431593 4_4:+14160313 Msa0671170:CDS
TTATTTGCAGGTTATTGATA+GGG 0.435162 4_4:+14161436 Msa0671170:intron
GGCACAAGATTTCAGGCAGC+AGG 0.435566 4_4:+14162017 Msa0671170:intron
GGGAGAGAAGATTGAGGTGT+TGG 0.441727 4_4:+14161457 Msa0671170:CDS
ATTCGGCTCCGGTCACCACC+AGG 0.447582 4_4:-14162498 None:intergenic
GCAAGTTTGCTAACTTCCTC+CGG 0.449150 4_4:-14160332 None:intergenic
TTGTGAATTGTGATCATTCA+TGG 0.455075 4_4:+14158873 Msa0671170:exon
GTAAACCTGTTGTTAGAAGC+AGG 0.458806 4_4:-14159009 None:intergenic
TGAAGTCAAAGGAGTTATGA+TGG 0.459537 4_4:+14160376 Msa0671170:CDS
CCAGGGTCAGGGCAGGCCTC+CGG 0.467838 4_4:-14162480 None:intergenic
AGAGAAGATTGAGGTGTTGG+TGG 0.479742 4_4:+14161460 Msa0671170:CDS
CTGTAAGCGCCCTTTCGGTC+CGG 0.482777 4_4:+14162461 Msa0671170:three_prime_UTR
CACAACCTTCCGAGGGGAAG+TGG 0.483080 4_4:-14162381 None:intergenic
CCGGAGGCCTGCCCTGACCC+TGG 0.483325 4_4:+14162480 Msa0671170:three_prime_UTR
ATTGTTCTTTCTGTGTGCCA+TGG 0.487473 4_4:+14162126 Msa0671170:CDS
ATCTATAGCTTTGTGGCTCG+TGG 0.490761 4_4:+14158910 Msa0671170:CDS
AGTTTAGGTGGATTCGGCTC+CGG 0.492682 4_4:-14162509 None:intergenic
TGGCTCAGTTGGTAAGGAAC+GGG 0.493822 4_4:+14162358 Msa0671170:three_prime_UTR
ATGCTTTAGACCAAAGTTGA+AGG 0.496438 4_4:+14160283 Msa0671170:intron
TGATAGGGGAGAGAAGATTG+AGG 0.498472 4_4:+14161451 Msa0671170:CDS
AAAGTCTTAACAAAGAATTT+GGG 0.498770 4_4:+14160185 Msa0671170:CDS
AAAATGATGAAGAAATGAAA+AGG 0.501975 4_4:-14158785 None:intergenic
CCAACACACGTTGGCTCAGT+TGG 0.513561 4_4:+14162347 Msa0671170:three_prime_UTR
GTCACCACCAGGGTCAGGGC+AGG 0.516535 4_4:-14162487 None:intergenic
TATTTGCAGGTTATTGATAG+GGG 0.520975 4_4:+14161437 Msa0671170:intron
GGAAGTGAATAAGGTGTGGT+AGG 0.525716 4_4:+14162298 Msa0671170:three_prime_UTR
ACACGTTGGCTCAGTTGGTA+AGG 0.528013 4_4:+14162352 Msa0671170:three_prime_UTR
TCCGGTCACCACCAGGGTCA+GGG 0.532476 4_4:-14162491 None:intergenic
TGATGTGAGGATGGTGTTGG+TGG 0.538282 4_4:+14162421 Msa0671170:three_prime_UTR
AGGTTTACTTACAACTGTGA+TGG 0.540715 4_4:+14159024 Msa0671170:CDS
TTTAATAAAAGATATGGTGG+AGG 0.542291 4_4:+14160139 Msa0671170:CDS
ATCGAACACACAACCTTCCG+AGG 0.552585 4_4:-14162389 None:intergenic
GATGTGAGGATGGTGTTGGT+GGG 0.554623 4_4:+14162422 Msa0671170:three_prime_UTR
GGGCAGGCCTCCGGACCGAA+AGG 0.557828 4_4:-14162471 None:intergenic
TTGAAGACACTGTAAAGCAA+TGG 0.558595 4_4:-14158980 None:intergenic
GGCAGGCCTCCGGACCGAAA+GGG 0.562834 4_4:-14162470 None:intergenic
GGCAGCAGGGAACCCAGTTG+AGG 0.565308 4_4:+14162031 Msa0671170:CDS
AGAGGTGGTGTCAAATTTGG+AGG 0.566987 4_4:-14158724 None:intergenic
ACGGGCCACTTCCCCTCGGA+AGG 0.568566 4_4:+14162376 Msa0671170:three_prime_UTR
TGCAGCAGATTCAATAGAAG+AGG 0.571940 4_4:-14158845 None:intergenic
GTTGTAGCCGTGGAGTCTGT+TGG 0.571948 4_4:+14160073 Msa0671170:CDS
GCGGAGTACAGTGACTTCAC+AGG 0.572690 4_4:+14158946 Msa0671170:CDS
TTGGCTCAGTTGGTAAGGAA+CGG 0.573450 4_4:+14162357 Msa0671170:three_prime_UTR
TAAGTTAACAACTGAAGCCA+TGG 0.574043 4_4:-14162143 None:intergenic
ACCATCCTCACATCAGCAGC+GGG 0.575616 4_4:-14162413 None:intergenic
TAAACCTGTTGTTAGAAGCA+GGG 0.578456 4_4:-14159008 None:intergenic
TCAATGCAATTATGATAGCA+AGG 0.581523 4_4:-14162095 None:intergenic
CCAACTGAGCCAACGTGTGT+TGG 0.585359 4_4:-14162347 None:intergenic
GCTCAAGTTTCTGAAGTCAA+AGG 0.586109 4_4:+14160365 Msa0671170:CDS
TAAGCGCCCTTTCGGTCCGG+AGG 0.593146 4_4:+14162464 Msa0671170:three_prime_UTR
CCACATCTTTCTCCTCAACT+GGG 0.594648 4_4:-14162043 None:intergenic
TTCGGCTCCGGTCACCACCA+GGG 0.595826 4_4:-14162497 None:intergenic
TCCCGCTGCTGATGTGAGGA+TGG 0.597281 4_4:+14162412 Msa0671170:three_prime_UTR
TGCTGATGTGAGGATGGTGT+TGG 0.599666 4_4:+14162418 Msa0671170:three_prime_UTR
TGTGAATTGTGATCATTCAT+GGG 0.599877 4_4:+14158874 Msa0671170:exon
TTGTCTACCAACAGACTCCA+CGG 0.608206 4_4:-14160080 None:intergenic
TCGTGGTACCGTGATTCTTG+CGG 0.609688 4_4:+14158927 Msa0671170:CDS
ACTGTACTCCGCAAGAATCA+CGG 0.610685 4_4:-14158935 None:intergenic
AGAATCTAACCTGAGAGCGA+AGG 0.618405 4_4:-14161496 None:intergenic
GAAGGGAAGTGAATAAGGTG+TGG 0.619277 4_4:+14162294 Msa0671170:three_prime_UTR
GCAGCAGATTCAATAGAAGA+GGG 0.624397 4_4:-14158844 None:intergenic
CGATTCCCGCTGCTGATGTG+AGG 0.625265 4_4:+14162408 Msa0671170:three_prime_UTR
GCACAAGATTTCAGGCAGCA+GGG 0.625497 4_4:+14162018 Msa0671170:CDS
GACCGAAAGGGCGCTTACAG+AGG 0.626244 4_4:-14162458 None:intergenic
CACCATCCTCACATCAGCAG+CGG 0.632218 4_4:-14162414 None:intergenic
AGGAACGGGCCACTTCCCCT+CGG 0.634180 4_4:+14162372 Msa0671170:three_prime_UTR
AGCTTACTGTGTTGTAGCCG+TGG 0.638922 4_4:+14160063 Msa0671170:intron
ATTATCGACGAGGAAGCTGA+AGG 0.649726 4_4:-14159052 None:intergenic
TCGAACACACAACCTTCCGA+GGG 0.650477 4_4:-14162388 None:intergenic
GAGGCCTGCCCTGACCCTGG+TGG 0.664380 4_4:+14162483 Msa0671170:three_prime_UTR
CCCAGTTGAGGAGAAAGATG+TGG 0.669727 4_4:+14162043 Msa0671170:CDS
GGAGCAAATGCAATATTGTG+TGG 0.673674 4_4:+14160304 Msa0671170:CDS
AAGACACTGTAAAGCAATGG+TGG 0.700756 4_4:-14158977 None:intergenic
ATATTGTGTGGAACATCCGG+AGG 0.702619 4_4:+14160316 Msa0671170:CDS
TACTGAATCCATTATCGACG+AGG 0.708046 4_4:-14159062 None:intergenic
TATGATGGAAAATATCGACA+AGG 0.735916 4_4:+14160391 Msa0671170:CDS
CGAACACACAACCTTCCGAG+GGG 0.760573 4_4:-14162387 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTACAAAAAGTTT+AGG - chr4_4:14162527-14162546 None:intergenic 10.0%
!!! TTTATTTTTTGGAAAAGAAA+TGG - chr4_4:14158811-14158830 None:intergenic 15.0%
!! TTGAACAAAAAATTATTCAA+GGG - chr4_4:14159140-14159159 None:intergenic 15.0%
!!! AAGAAAAAATCTCATATTTT+TGG - chr4_4:14159327-14159346 None:intergenic 15.0%
!! ATTGAAAAAAACAAAATGTA+AGG - chr4_4:14159789-14159808 None:intergenic 15.0%
!!! TCATAAATTTTAAGATAAGA+GGG - chr4_4:14159942-14159961 None:intergenic 15.0%
!!! ATCATAAATTTTAAGATAAG+AGG - chr4_4:14159943-14159962 None:intergenic 15.0%
!!! TTCATCTACATATATTTTTT+TGG + chr4_4:14159972-14159991 Msa0671170:intron 15.0%
!!! GATGATTTTAATAAAAGATA+TGG + chr4_4:14160133-14160152 Msa0671170:CDS 15.0%
!! AAATTTCATATAGTCATAAA+TGG - chr4_4:14161111-14161130 None:intergenic 15.0%
!!! TATTATTATATGTTATTTGC+AGG + chr4_4:14161424-14161443 Msa0671170:intron 15.0%
!! AAAATGATGAAGAAATGAAA+AGG - chr4_4:14158788-14158807 None:intergenic 20.0%
!! GTTGAACAAAAAATTATTCA+AGG - chr4_4:14159141-14159160 None:intergenic 20.0%
!!! TTAAACTGAACATTTTTACT+AGG + chr4_4:14159178-14159197 Msa0671170:intron 20.0%
!!! TAAACTGAACATTTTTACTA+GGG + chr4_4:14159179-14159198 Msa0671170:intron 20.0%
!! AAATCTAACATAATTAACGT+AGG + chr4_4:14159298-14159317 Msa0671170:intron 20.0%
!!! TAATCTGATTTTATGAATTG+AGG - chr4_4:14159718-14159737 None:intergenic 20.0%
!! ATAAGTAATTTAAGCAAGTA+AGG - chr4_4:14159897-14159916 None:intergenic 20.0%
!! TTACTTGCTTAAATTACTTA+TGG + chr4_4:14159896-14159915 Msa0671170:intron 20.0%
!!! GATTTTAATAAAAGATATGG+TGG + chr4_4:14160136-14160155 Msa0671170:CDS 20.0%
!! AAAAGTCTTAACAAAGAATT+TGG + chr4_4:14160184-14160203 Msa0671170:CDS 20.0%
!! AAAGTCTTAACAAAGAATTT+GGG + chr4_4:14160185-14160204 Msa0671170:CDS 20.0%
!!! TAGATTTTAAGATTTATGTC+GGG - chr4_4:14160538-14160557 None:intergenic 20.0%
!!! CTAGATTTTAAGATTTATGT+CGG - chr4_4:14160539-14160558 None:intergenic 20.0%
!!! ATTGACTCATATCATAAAAA+TGG - chr4_4:14160701-14160720 None:intergenic 20.0%
!!! CTTTTATGTATTTATAGAAC+CGG - chr4_4:14160765-14160784 None:intergenic 20.0%
!! CAAAGAAATCATCAATTATA+TGG - chr4_4:14160874-14160893 None:intergenic 20.0%
!! TCAGTTGTTTAACATTATTA+TGG + chr4_4:14161028-14161047 Msa0671170:intron 20.0%
!!! TATTTTTGTAGAATCGATAT+AGG - chr4_4:14161208-14161227 None:intergenic 20.0%
!! GAATTTCATTTAATATCATG+TGG + chr4_4:14161391-14161410 Msa0671170:intron 20.0%
!! ATTTGCATATATAAAACTGT+TGG - chr4_4:14161560-14161579 None:intergenic 20.0%
!! AATTGAATTAGAGATGATTA+TGG + chr4_4:14161577-14161596 Msa0671170:intron 20.0%
!!! ATTCCAATGTTTTATAGTTT+TGG + chr4_4:14161763-14161782 Msa0671170:intron 20.0%
!! TCCATTAAATTCTGAATTAT+TGG + chr4_4:14161965-14161984 Msa0671170:intron 20.0%
!! TCCAATAATTCAGAATTTAA+TGG - chr4_4:14161969-14161988 None:intergenic 20.0%
!! TTTGTTTATTAGACATTCAA+TGG + chr4_4:14162250-14162269 Msa0671170:three_prime_UTR 20.0%
!!! TTTTTTACAAAAAGTTTAGG+TGG - chr4_4:14162524-14162543 None:intergenic 20.0%
!! AAACTGTATTGTTTCATTTA+TGG + chr4_4:14162546-14162565 Msa0671170:three_prime_UTR 20.0%
!!! TAGGATATCACAGTTTTATA+TGG + chr4_4:14159210-14159229 Msa0671170:intron 25.0%
! TTTAGACAAAACCAAAAACA+AGG + chr4_4:14159249-14159268 Msa0671170:intron 25.0%
!!! TTTTAATTGTGATGATGAAG+TGG + chr4_4:14159380-14159399 Msa0671170:intron 25.0%
!!! TGTATTTTGAGCAATGTATA+AGG + chr4_4:14159444-14159463 Msa0671170:intron 25.0%
! AAAATTATCCAATGATGCTT+TGG + chr4_4:14159631-14159650 Msa0671170:intron 25.0%
!! TTTTATTAGTGAGCTTCAAA+TGG - chr4_4:14159823-14159842 None:intergenic 25.0%
! AAACAAGTTCAACAGAAAAA+AGG - chr4_4:14160023-14160042 None:intergenic 25.0%
! TTTAATAAAAGATATGGTGG+AGG + chr4_4:14160139-14160158 Msa0671170:CDS 25.0%
! TTCATAGCAGTATAAAAAAC+AGG + chr4_4:14160594-14160613 Msa0671170:intron 25.0%
!!! GATATGAGTCAATTTTAACT+CGG + chr4_4:14160708-14160727 Msa0671170:intron 25.0%
! AACTGAGACAAAATACTTAA+AGG - chr4_4:14161014-14161033 None:intergenic 25.0%
! AAAATCGCAATCAAAATTGA+TGG - chr4_4:14161172-14161191 None:intergenic 25.0%
!!! TCGATTTTGTTATTTCCTTA+TGG + chr4_4:14161270-14161289 Msa0671170:intron 25.0%
!! AATGAAATTCTAGAGACAAT+TGG - chr4_4:14161381-14161400 None:intergenic 25.0%
! TTATTTGCAGGTTATTGATA+GGG + chr4_4:14161436-14161455 Msa0671170:intron 25.0%
!! CATATATAAAACTGTTGGTT+TGG - chr4_4:14161555-14161574 None:intergenic 25.0%
! CATCCAAAACTATAAAACAT+TGG - chr4_4:14161769-14161788 None:intergenic 25.0%
!!! CAATGTTTTATAGTTTTGGA+TGG + chr4_4:14161767-14161786 Msa0671170:intron 25.0%
! AAAACTAGATGTGAGAATTT+GGG - chr4_4:14161845-14161864 None:intergenic 25.0%
! GTTGTGGTTATTTATTATCT+AGG + chr4_4:14161912-14161931 Msa0671170:intron 25.0%
! TATTGGAATGTCAATTGAAA+TGG + chr4_4:14161982-14162001 Msa0671170:intron 25.0%
! AGAATTGTCACTACATATAT+TGG + chr4_4:14162180-14162199 Msa0671170:three_prime_UTR 25.0%
! ATGTAAAAAACAGTTAGGAA+GGG + chr4_4:14162277-14162296 Msa0671170:three_prime_UTR 25.0%
TTGTGAATTGTGATCATTCA+TGG + chr4_4:14158873-14158892 Msa0671170:exon 30.0%
TGTGAATTGTGATCATTCAT+GGG + chr4_4:14158874-14158893 Msa0671170:exon 30.0%
! ATCATTGATCTATAGCTTTG+TGG + chr4_4:14158903-14158922 Msa0671170:CDS 30.0%
! AAAAATCTCCAAAGCATCAT+TGG - chr4_4:14159642-14159661 None:intergenic 30.0%
! TGAACTTGTTTCATGATTAG+TGG + chr4_4:14160032-14160051 Msa0671170:intron 30.0%
ATACGTTCAAGAAAAGCTAT+TGG - chr4_4:14160109-14160128 None:intergenic 30.0%
! AGCTTTTCTTGAACGTATTA+AGG + chr4_4:14160111-14160130 Msa0671170:CDS 30.0%
! TATGATGGAAAATATCGACA+AGG + chr4_4:14160391-14160410 Msa0671170:CDS 30.0%
!!! AAATGTAGGTGCTATTTTGT+TGG - chr4_4:14160457-14160476 None:intergenic 30.0%
! GGTTCTTCAATCTAAAATGT+AGG - chr4_4:14160471-14160490 None:intergenic 30.0%
TTTAGATTGAAGAACCGAAT+CGG + chr4_4:14160475-14160494 Msa0671170:intron 30.0%
! ATAACATGTAGATACCGATT+CGG - chr4_4:14160492-14160511 None:intergenic 30.0%
TATTTATAGAACCGGATCAT+AGG - chr4_4:14160757-14160776 None:intergenic 30.0%
! TTTTATTCTTTCACACCACA+CGG + chr4_4:14160795-14160814 Msa0671170:intron 30.0%
! TCTTTTGCCTTGAAACTTAA+TGG + chr4_4:14160940-14160959 Msa0671170:intron 30.0%
TAAGTGACCATTAAGTTTCA+AGG - chr4_4:14160950-14160969 None:intergenic 30.0%
AAATATTTCGTCAGACATGT+AGG + chr4_4:14160983-14161002 Msa0671170:intron 30.0%
TATGGCTACTAAAGAATATC+TGG + chr4_4:14161046-14161065 Msa0671170:intron 30.0%
AAAATCGCAATCAAAATCGA+AGG - chr4_4:14161243-14161262 None:intergenic 30.0%
GTTATTTGCAGGTTATTGAT+AGG + chr4_4:14161435-14161454 Msa0671170:intron 30.0%
TATTTGCAGGTTATTGATAG+GGG + chr4_4:14161437-14161456 Msa0671170:intron 30.0%
! TTGTTTGGTTTAAATGTGAG+TGG + chr4_4:14161684-14161703 Msa0671170:intron 30.0%
!!! TATAGTTTTGGATGGAATTG+TGG + chr4_4:14161775-14161794 Msa0671170:intron 30.0%
GAAAACTAGATGTGAGAATT+TGG - chr4_4:14161846-14161865 None:intergenic 30.0%
GAATGTCAATTGAAATGGTA+TGG + chr4_4:14161987-14162006 Msa0671170:intron 30.0%
TCAATGCAATTATGATAGCA+AGG - chr4_4:14162098-14162117 None:intergenic 30.0%
GCTTCATGTAAAAAACAGTT+AGG + chr4_4:14162272-14162291 Msa0671170:three_prime_UTR 30.0%
CATGTAAAAAACAGTTAGGA+AGG + chr4_4:14162276-14162295 Msa0671170:three_prime_UTR 30.0%
! ACAAAAAGTTTAGGTGGATT+CGG - chr4_4:14162518-14162537 None:intergenic 30.0%
TTGAAGACACTGTAAAGCAA+TGG - chr4_4:14158983-14159002 None:intergenic 35.0%
TAAACCTGTTGTTAGAAGCA+GGG - chr4_4:14159011-14159030 None:intergenic 35.0%
AGGTTTACTTACAACTGTGA+TGG + chr4_4:14159024-14159043 Msa0671170:CDS 35.0%
! TTTTACTAGGGTTCGAACTT+AGG + chr4_4:14159191-14159210 Msa0671170:intron 35.0%
!!! GCACTTATGTACCTTGTTTT+TGG - chr4_4:14159263-14159282 None:intergenic 35.0%
CATCCAATAATAGAAGTGGA+CGG - chr4_4:14159407-14159426 None:intergenic 35.0%
TGAGCAATGTATAAGGTCAA+TGG + chr4_4:14159451-14159470 Msa0671170:intron 35.0%
TAGGTGGAACAATAACTAGT+TGG + chr4_4:14159489-14159508 Msa0671170:intron 35.0%
! ATGCTTTAGACCAAAGTTGA+AGG + chr4_4:14160283-14160302 Msa0671170:intron 35.0%
TGAAGTCAAAGGAGTTATGA+TGG + chr4_4:14160376-14160395 Msa0671170:CDS 35.0%
GAAAATCCATGCAAAATCGT+AGG + chr4_4:14160658-14160677 Msa0671170:intron 35.0%
! CAAAATCCTACGATTTTGCA+TGG - chr4_4:14160667-14160686 None:intergenic 35.0%
! GCAAAATCGTAGGATTTTGA+AGG + chr4_4:14160668-14160687 Msa0671170:intron 35.0%
! TTTCTTTGTGATTTGCATCG+AGG + chr4_4:14160886-14160905 Msa0671170:intron 35.0%
CTAAAGAATATCTGGCATCT+AGG + chr4_4:14161054-14161073 Msa0671170:intron 35.0%
! ATCTAGGCTTGAGTATTTTG+TGG + chr4_4:14161070-14161089 Msa0671170:intron 35.0%
TTCAGGTCACAAAATCCATA+AGG - chr4_4:14161288-14161307 None:intergenic 35.0%
! GACAGTAGGTTTTCAAAATC+GGG - chr4_4:14161318-14161337 None:intergenic 35.0%
! GGACAGTAGGTTTTCAAAAT+CGG - chr4_4:14161319-14161338 None:intergenic 35.0%
!! GATTTTGAAAACCTACTGTC+CGG + chr4_4:14161318-14161337 Msa0671170:intron 35.0%
ACTCGAATGATGTCTTTGTT+TGG + chr4_4:14161669-14161688 Msa0671170:intron 35.0%
ATTGTGGTGTCTAAATCTCT+TGG + chr4_4:14161791-14161810 Msa0671170:intron 35.0%
!!! GAGCTTAGCTTATTTTGTTG+TGG + chr4_4:14161896-14161915 Msa0671170:intron 35.0%
TAAGTTAACAACTGAAGCCA+TGG - chr4_4:14162146-14162165 None:intergenic 35.0%
! TTTTTATTGCCAACACACGT+TGG + chr4_4:14162338-14162357 Msa0671170:three_prime_UTR 35.0%
AGATATAGAGAGAGAGAGAG+AGG - chr4_4:14158745-14158764 None:intergenic 40.0%
GCAGCAGATTCAATAGAAGA+GGG - chr4_4:14158847-14158866 None:intergenic 40.0%
TGCAGCAGATTCAATAGAAG+AGG - chr4_4:14158848-14158867 None:intergenic 40.0%
AAGACACTGTAAAGCAATGG+TGG - chr4_4:14158980-14158999 None:intergenic 40.0%
GTAAACCTGTTGTTAGAAGC+AGG - chr4_4:14159012-14159031 None:intergenic 40.0%
TACTGAATCCATTATCGACG+AGG - chr4_4:14159065-14159084 None:intergenic 40.0%
CGTCCACTTCTATTATTGGA+TGG + chr4_4:14159405-14159424 Msa0671170:intron 40.0%
CCACCATCCAATAATAGAAG+TGG - chr4_4:14159411-14159430 None:intergenic 40.0%
CCACTTCTATTATTGGATGG+TGG + chr4_4:14159408-14159427 Msa0671170:intron 40.0%
TAAGGTCAATGGTCCAAACT+TGG + chr4_4:14159462-14159481 Msa0671170:intron 40.0%
ATGGTCCAAACTTGGCTAAT+AGG + chr4_4:14159470-14159489 Msa0671170:intron 40.0%
TTCCACCTATTAGCCAAGTT+TGG - chr4_4:14159478-14159497 None:intergenic 40.0%
TGCATTTGCTCCTTCAACTT+TGG - chr4_4:14160296-14160315 None:intergenic 40.0%
GGAGCAAATGCAATATTGTG+TGG + chr4_4:14160304-14160323 Msa0671170:CDS 40.0%
GCAATATTGTGTGGAACATC+CGG + chr4_4:14160313-14160332 Msa0671170:CDS 40.0%
!! GCTCAAGTTTCTGAAGTCAA+AGG + chr4_4:14160365-14160384 Msa0671170:CDS 40.0%
! CAAAATCGGGATTTAGCTTC+AGG - chr4_4:14161305-14161324 None:intergenic 40.0%
! GCAAAGCACCTAAACATACA+CGG - chr4_4:14161347-14161366 None:intergenic 40.0%
TCGAATTCCAGTGATCTCTA+AGG - chr4_4:14161878-14161897 None:intergenic 40.0%
! TTGAAATGGTATGGCTTTGC+AGG + chr4_4:14161996-14162015 Msa0671170:intron 40.0%
ATTGTTCTTTCTGTGTGCCA+TGG + chr4_4:14162126-14162145 Msa0671170:CDS 40.0%
GTTAGGAAGGGAAGTGAATA+AGG + chr4_4:14162289-14162308 Msa0671170:three_prime_UTR 40.0%
! GAGAGAGGTGGTGTCAAATT+TGG - chr4_4:14158730-14158749 None:intergenic 45.0%
TATAGAGAGAGAGAGAGAGG+TGG - chr4_4:14158742-14158761 None:intergenic 45.0%
!! ATCTATAGCTTTGTGGCTCG+TGG + chr4_4:14158910-14158929 Msa0671170:CDS 45.0%
ACTGTACTCCGCAAGAATCA+CGG - chr4_4:14158938-14158957 None:intergenic 45.0%
AACTCCCTGCTTCTAACAAC+AGG + chr4_4:14159004-14159023 Msa0671170:CDS 45.0%
ATTATCGACGAGGAAGCTGA+AGG - chr4_4:14159055-14159074 None:intergenic 45.0%
TTCAGCTTCCTCGTCGATAA+TGG + chr4_4:14159054-14159073 Msa0671170:CDS 45.0%
GGACCGTCCACTTCTATTAT+TGG + chr4_4:14159401-14159420 Msa0671170:intron 45.0%
GTCCAAACTTGGCTAATAGG+TGG + chr4_4:14159473-14159492 Msa0671170:intron 45.0%
TTGTCTACCAACAGACTCCA+CGG - chr4_4:14160083-14160102 None:intergenic 45.0%
ATATTGTGTGGAACATCCGG+AGG + chr4_4:14160316-14160335 Msa0671170:CDS 45.0%
!! GCAAGTTTGCTAACTTCCTC+CGG - chr4_4:14160335-14160354 None:intergenic 45.0%
ACTCGGTTCATCAATTGACC+TGG + chr4_4:14160725-14160744 Msa0671170:intron 45.0%
! CCTGGTTTGTACCTATGATC+CGG + chr4_4:14160743-14160762 Msa0671170:intron 45.0%
TCTGCACAAGAGATTGACGA+AGG + chr4_4:14160820-14160839 Msa0671170:intron 45.0%
! AGGCGAACGTGTTGATATGA+TGG + chr4_4:14160840-14160859 Msa0671170:intron 45.0%
ACCTAAACATACACGGACAC+CGG - chr4_4:14161340-14161359 None:intergenic 45.0%
TGATAGGGGAGAGAAGATTG+AGG + chr4_4:14161451-14161470 Msa0671170:CDS 45.0%
!! AGAGAAGATTGAGGTGTTGG+TGG + chr4_4:14161460-14161479 Msa0671170:CDS 45.0%
AGAATCTAACCTGAGAGCGA+AGG - chr4_4:14161499-14161518 None:intergenic 45.0%
GTTGATGCCTTAGAGATCAC+TGG + chr4_4:14161868-14161887 Msa0671170:intron 45.0%
CTTTGCAGGCACAAGATTTC+AGG + chr4_4:14162010-14162029 Msa0671170:intron 45.0%
CCACATCTTTCTCCTCAACT+GGG - chr4_4:14162046-14162065 None:intergenic 45.0%
ACCACATCTTTCTCCTCAAC+TGG - chr4_4:14162047-14162066 None:intergenic 45.0%
GAAGGGAAGTGAATAAGGTG+TGG + chr4_4:14162294-14162313 Msa0671170:three_prime_UTR 45.0%
GGAAGTGAATAAGGTGTGGT+AGG + chr4_4:14162298-14162317 Msa0671170:three_prime_UTR 45.0%
!! TTGGCTCAGTTGGTAAGGAA+CGG + chr4_4:14162357-14162376 Msa0671170:three_prime_UTR 45.0%
!!! AGATTTTTTATTTTATTTTT+TGG - chr4_4:14158822-14158841 None:intergenic 5.0%
!! TATATTTAATTAATGTTTAA+TGG + chr4_4:14159520-14159539 Msa0671170:intron 5.0%
!!! TAAAAATAAAATATGATTTT+AGG + chr4_4:14159598-14159617 Msa0671170:intron 5.0%
TCGTGGTACCGTGATTCTTG+CGG + chr4_4:14158927-14158946 Msa0671170:CDS 50.0%
AGCTTACTGTGTTGTAGCCG+TGG + chr4_4:14160063-14160082 Msa0671170:intron 50.0%
CCGGATCATAGGTACAAACC+AGG - chr4_4:14160746-14160765 None:intergenic 50.0%
TCCGGTGTCCGTGTATGTTT+AGG + chr4_4:14161336-14161355 Msa0671170:intron 50.0%
GGGAGAGAAGATTGAGGTGT+TGG + chr4_4:14161457-14161476 Msa0671170:CDS 50.0%
AACTGAGAACCTTCGCTCTC+AGG + chr4_4:14161487-14161506 Msa0671170:CDS 50.0%
TTGGGAGCAACTCGATGATC+AGG - chr4_4:14161827-14161846 None:intergenic 50.0%
GCACAAGATTTCAGGCAGCA+GGG + chr4_4:14162018-14162037 Msa0671170:CDS 50.0%
CCCAGTTGAGGAGAAAGATG+TGG + chr4_4:14162043-14162062 Msa0671170:CDS 50.0%
!! ACACGTTGGCTCAGTTGGTA+AGG + chr4_4:14162352-14162371 Msa0671170:three_prime_UTR 50.0%
!! TGGCTCAGTTGGTAAGGAAC+GGG + chr4_4:14162358-14162377 Msa0671170:three_prime_UTR 50.0%
TCGAACACACAACCTTCCGA+GGG - chr4_4:14162391-14162410 None:intergenic 50.0%
ATCGAACACACAACCTTCCG+AGG - chr4_4:14162392-14162411 None:intergenic 50.0%
TGCTGATGTGAGGATGGTGT+TGG + chr4_4:14162418-14162437 Msa0671170:three_prime_UTR 50.0%
! TGATGTGAGGATGGTGTTGG+TGG + chr4_4:14162421-14162440 Msa0671170:three_prime_UTR 50.0%
!! GATGTGAGGATGGTGTTGGT+GGG + chr4_4:14162422-14162441 Msa0671170:three_prime_UTR 50.0%
TACCTCTGTAAGCGCCCTTT+CGG + chr4_4:14162456-14162475 Msa0671170:three_prime_UTR 50.0%
! AGTTTAGGTGGATTCGGCTC+CGG - chr4_4:14162512-14162531 None:intergenic 50.0%
GCGGAGTACAGTGACTTCAC+AGG + chr4_4:14158946-14158965 Msa0671170:CDS 55.0%
GTTGTAGCCGTGGAGTCTGT+TGG + chr4_4:14160073-14160092 Msa0671170:CDS 55.0%
TCTCTTGTGCAGAGACCGTG+TGG - chr4_4:14160813-14160832 None:intergenic 55.0%
ATACACGGACACCGGACAGT+AGG - chr4_4:14161332-14161351 None:intergenic 55.0%
GGCACAAGATTTCAGGCAGC+AGG + chr4_4:14162017-14162036 Msa0671170:intron 55.0%
CCAACTGAGCCAACGTGTGT+TGG - chr4_4:14162350-14162369 None:intergenic 55.0%
!! CCAACACACGTTGGCTCAGT+TGG + chr4_4:14162347-14162366 Msa0671170:three_prime_UTR 55.0%
CGAACACACAACCTTCCGAG+GGG - chr4_4:14162390-14162409 None:intergenic 55.0%
ACCATCCTCACATCAGCAGC+GGG - chr4_4:14162416-14162435 None:intergenic 55.0%
CACCATCCTCACATCAGCAG+CGG - chr4_4:14162417-14162436 None:intergenic 55.0%
CACAACCTTCCGAGGGGAAG+TGG - chr4_4:14162384-14162403 None:intergenic 60.0%
CGATTCCCGCTGCTGATGTG+AGG + chr4_4:14162408-14162427 Msa0671170:three_prime_UTR 60.0%
TCCCGCTGCTGATGTGAGGA+TGG + chr4_4:14162412-14162431 Msa0671170:three_prime_UTR 60.0%
!! GACCGAAAGGGCGCTTACAG+AGG - chr4_4:14162461-14162480 None:intergenic 60.0%
CTGTAAGCGCCCTTTCGGTC+CGG + chr4_4:14162461-14162480 Msa0671170:three_prime_UTR 60.0%
GGCAGCAGGGAACCCAGTTG+AGG + chr4_4:14162031-14162050 Msa0671170:CDS 65.0%
AGGAACGGGCCACTTCCCCT+CGG + chr4_4:14162372-14162391 Msa0671170:three_prime_UTR 65.0%
TAAGCGCCCTTTCGGTCCGG+AGG + chr4_4:14162464-14162483 Msa0671170:three_prime_UTR 65.0%
TCCGGTCACCACCAGGGTCA+GGG - chr4_4:14162494-14162513 None:intergenic 65.0%
TTCGGCTCCGGTCACCACCA+GGG - chr4_4:14162500-14162519 None:intergenic 65.0%
ATTCGGCTCCGGTCACCACC+AGG - chr4_4:14162501-14162520 None:intergenic 65.0%
ACGGGCCACTTCCCCTCGGA+AGG + chr4_4:14162376-14162395 Msa0671170:three_prime_UTR 70.0%
GGCAGGCCTCCGGACCGAAA+GGG - chr4_4:14162473-14162492 None:intergenic 70.0%
GTCACCACCAGGGTCAGGGC+AGG - chr4_4:14162490-14162509 None:intergenic 70.0%
! GCCCTGACCCTGGTGGTGAC+CGG + chr4_4:14162490-14162509 Msa0671170:three_prime_UTR 70.0%
CTCCGGTCACCACCAGGGTC+AGG - chr4_4:14162495-14162514 None:intergenic 70.0%
GGGCAGGCCTCCGGACCGAA+AGG - chr4_4:14162474-14162493 None:intergenic 75.0%
CCAGGGTCAGGGCAGGCCTC+CGG - chr4_4:14162483-14162502 None:intergenic 75.0%
GAGGCCTGCCCTGACCCTGG+TGG + chr4_4:14162483-14162502 Msa0671170:three_prime_UTR 75.0%
! CCGGAGGCCTGCCCTGACCC+TGG + chr4_4:14162480-14162499 Msa0671170:three_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 14158727 14162575 14158727 ID=Msa0671170;Name=Msa0671170
chr4_4 mRNA 14158727 14162575 14158727 ID=Msa0671170-mRNA-1;Parent=Msa0671170;Name=Msa0671170-mRNA-1;_AED=0.04;_eAED=0.04;_QI=165|1|1|1|1|1|5|414|219
chr4_4 exon 14158727 14159081 14158727 ID=Msa0671170-mRNA-1:exon:4307;Parent=Msa0671170-mRNA-1
chr4_4 exon 14160065 14160206 14160065 ID=Msa0671170-mRNA-1:exon:4308;Parent=Msa0671170-mRNA-1
chr4_4 exon 14160292 14160412 14160292 ID=Msa0671170-mRNA-1:exon:4309;Parent=Msa0671170-mRNA-1
chr4_4 exon 14161446 14161508 14161446 ID=Msa0671170-mRNA-1:exon:4310;Parent=Msa0671170-mRNA-1
chr4_4 exon 14162018 14162575 14162018 ID=Msa0671170-mRNA-1:exon:4311;Parent=Msa0671170-mRNA-1
chr4_4 five_prime_UTR 14158727 14158891 14158727 ID=Msa0671170-mRNA-1:five_prime_utr;Parent=Msa0671170-mRNA-1
chr4_4 CDS 14158892 14159081 14158892 ID=Msa0671170-mRNA-1:cds;Parent=Msa0671170-mRNA-1
chr4_4 CDS 14160065 14160206 14160065 ID=Msa0671170-mRNA-1:cds;Parent=Msa0671170-mRNA-1
chr4_4 CDS 14160292 14160412 14160292 ID=Msa0671170-mRNA-1:cds;Parent=Msa0671170-mRNA-1
chr4_4 CDS 14161446 14161508 14161446 ID=Msa0671170-mRNA-1:cds;Parent=Msa0671170-mRNA-1
chr4_4 CDS 14162018 14162161 14162018 ID=Msa0671170-mRNA-1:cds;Parent=Msa0671170-mRNA-1
chr4_4 three_prime_UTR 14162162 14162575 14162162 ID=Msa0671170-mRNA-1:three_prime_utr;Parent=Msa0671170-mRNA-1
Gene Sequence

>Msa0671170

ATGGGACAACAATCATTGATCTATAGCTTTGTGGCTCGTGGTACCGTGATTCTTGCGGAGTACAGTGACTTCACAGGAAACTTCACCACCATTGCTTTACAGTGTCTTCAAAAACTCCCTGCTTCTAACAACAGGTTTACTTACAACTGTGATGGACATACCTTCAGCTTCCTCGTCGATAATGGATTCACTTACTGTGTTGTAGCCGTGGAGTCTGTTGGTAGACAAATTCCAATAGCTTTTCTTGAACGTATTAAGGATGATTTTAATAAAAGATATGGTGGAGGAAGAGCTACAACAGCAACTGCTAAAAGTCTTAACAAAGAATTTGGACCAAAGTTGAAGGAGCAAATGCAATATTGTGTGGAACATCCGGAGGAAGTTAGCAAACTTGCTAAAGTGAAAGCTCAAGTTTCTGAAGTCAAAGGAGTTATGATGGAAAATATCGACAAGGTTATTGATAGGGGAGAGAAGATTGAGGTGTTGGTGGATAAAACTGAGAACCTTCGCTCTCAGGCACAAGATTTCAGGCAGCAGGGAACCCAGTTGAGGAGAAAGATGTGGTATCAAAACATGAAGATCAAGCTCATAGTCCTTGCTATCATAATTGCATTGATTCTCATTATTGTTCTTTCTGTGTGCCATGGCTTCAGTTGTTAA
Protein sequence

>Msa0671170

MGQQSLIYSFVARGTVILAEYSDFTGNFTTIALQCLQKLPASNNRFTYNCDGHTFSFLVDNGFTYCVVAVESVGRQIPIAFLERIKDDFNKRYGGGRATTATAKSLNKEFGPKLKEQMQYCVEHPEEVSKLAKVKAQVSEVKGVMMENIDKVIDRGEKIEVLVDKTENLRSQAQDFRQQGTQLRRKMWYQNMKIKLIVLAIIIALILIIVLSVCHGFSC*