AlfalfaGEDB | Msa0317300

M. sativa cultivar zhongmu-4 / Msa0317300

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0317300	XP_013462938.1	97.211	251	7	0	1	251	1	251	5.06e-167	471

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011060	Msa0317300	-0.806598	7.612362e-50	-8.615850e-47
Msa0036560	Msa0317300	-0.818415	1.983516e-52	-8.615850e-47
Msa0043150	Msa0317300	0.814443	1.536742e-51	-8.615850e-47
Msa0045620	Msa0317300	0.810323	1.220216e-50	-8.615850e-47
Msa0048410	Msa0317300	-0.813762	2.171987e-51	-8.615850e-47
Msa0050320	Msa0317300	-0.842697	2.207133e-58	-8.615850e-47
Msa0060080	Msa0317300	-0.815399	9.428866e-52	-8.615850e-47
Msa0069480	Msa0317300	0.807600	4.670865e-50	-8.615850e-47
Msa0078990	Msa0317300	0.800111	1.680063e-48	-8.615850e-47
Msa0081290	Msa0317300	-0.812862	3.423618e-51	-8.615850e-47
Msa0099090	Msa0317300	-0.843786	1.131136e-58	-8.615850e-47
Msa0110240	Msa0317300	0.817665	2.931637e-52	-8.615850e-47
Msa0121460	Msa0317300	-0.816663	4.923136e-52	-8.615850e-47
Msa0131460	Msa0317300	0.802529	5.372327e-49	-8.615850e-47
Msa0132080	Msa0317300	-0.810908	9.123439e-51	-8.615850e-47
Msa0139280	Msa0317300	-0.818624	1.778424e-52	-8.615850e-47
Msa0154720	Msa0317300	-0.840900	6.580598e-58	-8.615850e-47
Msa0169710	Msa0317300	0.827540	1.478701e-54	-8.615850e-47
Msa0174590	Msa0317300	0.807034	6.156859e-50	-8.615850e-47
Msa0190890	Msa0317300	0.801313	9.550832e-49	-8.615850e-47
Msa0195030	Msa0317300	0.833716	4.544259e-56	-8.615850e-47
Msa0197920	Msa0317300	0.953240	4.238830e-111	-8.615850e-47
Msa0211460	Msa0317300	0.806581	7.672267e-50	-8.615850e-47
Msa0223590	Msa0317300	0.809300	2.025187e-50	-8.615850e-47
Msa0225070	Msa0317300	-0.811413	7.090069e-51	-8.615850e-47
Msa0225370	Msa0317300	-0.810146	1.332411e-50	-8.615850e-47
Msa0232660	Msa0317300	-0.810665	1.029560e-50	-8.615850e-47
Msa0235080	Msa0317300	0.826005	3.439358e-54	-8.615850e-47
Msa0237180	Msa0317300	0.837205	5.962716e-57	-8.615850e-47
Msa0264890	Msa0317300	0.813495	2.487016e-51	-8.615850e-47
Msa0266140	Msa0317300	-0.800246	1.576690e-48	-8.615850e-47
Msa0266380	Msa0317300	-0.835121	2.018130e-56	-8.615850e-47
Msa0273360	Msa0317300	0.800879	1.171528e-48	-8.615850e-47
Msa0274940	Msa0317300	0.811382	7.198033e-51	-8.615850e-47
Msa0277320	Msa0317300	0.920933	6.827352e-88	-8.615850e-47
Msa0290800	Msa0317300	0.830263	3.240193e-55	-8.615850e-47
Msa0311540	Msa0317300	0.806722	7.164584e-50	-8.615850e-47
Msa0315660	Msa0317300	0.837442	5.185931e-57	-8.615850e-47
Msa0317300	Msa0328870	0.835714	1.428835e-56	-8.615850e-47
Msa0317300	Msa0341860	-0.839713	1.343951e-57	-8.615850e-47
Msa0317300	Msa0354550	0.805746	1.150492e-49	-8.615850e-47
Msa0317300	Msa0372100	0.816055	6.735095e-52	-8.615850e-47
Msa0317300	Msa0375500	0.828958	6.729605e-55	-8.615850e-47
Msa0317300	Msa0378620	-0.830189	3.378941e-55	-8.615850e-47
Msa0317300	Msa0380590	0.832789	7.733606e-56	-8.615850e-47
Msa0317300	Msa0385030	0.806465	8.119576e-50	-8.615850e-47
Msa0317300	Msa0387890	0.805530	1.276746e-49	-8.615850e-47
Msa0317300	Msa0410960	-0.822313	2.532143e-53	-8.615850e-47
Msa0317300	Msa0425950	0.800760	1.239242e-48	-8.615850e-47
Msa0317300	Msa0426030	0.812527	4.053091e-51	-8.615850e-47
Msa0317300	Msa0426170	0.812855	3.435646e-51	-8.615850e-47
Msa0317300	Msa0432260	-0.846272	2.410570e-59	-8.615850e-47
Msa0317300	Msa0452120	-0.808498	3.006994e-50	-8.615850e-47
Msa0317300	Msa0452490	-0.821083	4.873948e-53	-8.615850e-47
Msa0317300	Msa0467250	0.837587	4.761398e-57	-8.615850e-47
Msa0317300	Msa0471210	0.809074	2.264766e-50	-8.615850e-47
Msa0317300	Msa0476730	-0.844751	6.228522e-59	-8.615850e-47
Msa0317300	Msa0482760	0.811783	5.890510e-51	-8.615850e-47
Msa0317300	Msa0483090	-0.803818	2.907049e-49	-8.615850e-47
Msa0317300	Msa0484410	0.806221	9.140301e-50	-8.615850e-47
Msa0317300	Msa0500420	0.835585	1.539929e-56	-8.615850e-47
Msa0317300	Msa0503620	-0.827695	1.357331e-54	-8.615850e-47
Msa0317300	Msa0521100	-0.825034	5.841667e-54	-8.615850e-47
Msa0317300	Msa0527560	0.953327	3.500211e-111	-8.615850e-47
Msa0317300	Msa0530660	0.815692	8.117469e-52	-8.615850e-47
Msa0317300	Msa0538650	0.823258	1.526032e-53	-8.615850e-47
Msa0317300	Msa0540160	-0.830247	3.270648e-55	-8.615850e-47
Msa0317300	Msa0549550	-0.807982	3.874478e-50	-8.615850e-47
Msa0317300	Msa0566460	0.826399	2.771619e-54	-8.615850e-47
Msa0317300	Msa0566930	0.823951	1.050258e-53	-8.615850e-47
Msa0317300	Msa0587180	0.808470	3.049139e-50	-8.615850e-47
Msa0317300	Msa0623310	0.816052	6.746087e-52	-8.615850e-47
Msa0317300	Msa0626980	0.817086	3.957511e-52	-8.615850e-47
Msa0317300	Msa0627360	0.810721	1.001389e-50	-8.615850e-47
Msa0317300	Msa0646180	-0.802113	6.543695e-49	-8.615850e-47
Msa0317300	Msa0658120	0.837878	4.007616e-57	-8.615850e-47
Msa0317300	Msa0659020	-0.807988	3.861781e-50	-8.615850e-47
Msa0317300	Msa0671400	0.822915	1.834316e-53	-8.615850e-47
Msa0317300	Msa0673940	0.817805	2.725507e-52	-8.615850e-47
Msa0317300	Msa0678620	0.809638	1.713684e-50	-8.615850e-47
Msa0317300	Msa0685390	-0.808942	2.417319e-50	-8.615850e-47
Msa0317300	Msa0702970	0.840263	9.660160e-58	-8.615850e-47
Msa0317300	Msa0716700	0.821754	3.412615e-53	-8.615850e-47
Msa0317300	Msa0733240	-0.802788	4.751085e-49	-8.615850e-47
Msa0317300	Msa0746090	0.804471	2.126250e-49	-8.615850e-47
Msa0317300	Msa0754750	0.817570	3.078992e-52	-8.615850e-47
Msa0317300	Msa0772420	0.808585	2.880716e-50	-8.615850e-47
Msa0317300	Msa0777210	0.801894	7.257796e-49	-8.615850e-47
Msa0317300	Msa0797980	-0.803637	3.170301e-49	-8.615850e-47
Msa0317300	Msa0828350	0.813295	2.751996e-51	-8.615850e-47
Msa0317300	Msa0829770	-0.811419	7.067074e-51	-8.615850e-47
Msa0317300	Msa0846900	0.806176	9.341436e-50	-8.615850e-47
Msa0317300	Msa0852620	0.811035	8.562642e-51	-8.615850e-47
Msa0317300	Msa0870220	-0.817692	2.889583e-52	-8.615850e-47
Msa0317300	Msa0880050	0.819536	1.103024e-52	-8.615850e-47
Msa0317300	Msa0898830	-0.807633	4.595023e-50	-8.615850e-47
Msa0317300	Msa0903460	-0.811925	5.486452e-51	-8.615850e-47
Msa0317300	Msa0907170	0.815912	7.249813e-52	-8.615850e-47
Msa0317300	Msa0935930	-0.836031	1.187101e-56	-8.615850e-47
Msa0317300	Msa0962290	0.801349	9.390265e-49	-8.615850e-47
Msa0317300	Msa0967900	-0.809118	2.215782e-50	-8.615850e-47
Msa0317300	Msa0972930	-0.820993	5.114347e-53	-8.615850e-47
Msa0317300	Msa0982560	0.800822	1.203343e-48	-8.615850e-47
Msa0317300	Msa0984820	-0.806478	8.066490e-50	-8.615850e-47
Msa0317300	Msa0987670	0.816984	4.172449e-52	-8.615850e-47
Msa0317300	Msa1052030	-0.805126	1.551692e-49	-8.615850e-47
Msa0317300	Msa1070630	-0.800325	1.519315e-48	-8.615850e-47
Msa0317300	Msa1078300	0.801256	9.808719e-49	-8.615850e-47
Msa0317300	Msa1088260	0.810219	1.285064e-50	-8.615850e-47
Msa0317300	Msa1093190	-0.828401	9.175327e-55	-8.615850e-47
Msa0317300	Msa1109880	0.800504	1.397407e-48	-8.615850e-47
Msa0317300	Msa1124980	0.810647	1.038865e-50	-8.615850e-47
Msa0317300	Msa1127200	0.802730	4.883118e-49	-8.615850e-47
Msa0317300	Msa1145930	-0.800346	1.504926e-48	-8.615850e-47
Msa0317300	Msa1146980	0.812788	3.554338e-51	-8.615850e-47
Msa0317300	Msa1150870	0.813597	2.361442e-51	-8.615850e-47
Msa0317300	Msa1167360	0.818830	1.597469e-52	-8.615850e-47
Msa0317300	Msa1170320	0.803686	3.097264e-49	-8.615850e-47
Msa0317300	Msa1186260	0.849203	3.760574e-60	-8.615850e-47
Msa0317300	Msa1199750	-0.800714	1.265862e-48	-8.615850e-47
Msa0317300	Msa1200360	0.806527	7.876836e-50	-8.615850e-47
Msa0317300	Msa1214960	0.828940	6.798329e-55	-8.615850e-47
Msa0317300	Msa1235590	0.815431	9.279308e-52	-8.615850e-47
Msa0317300	Msa1251500	0.801308	9.571012e-49	-8.615850e-47
Msa0317300	Msa1253270	0.811367	7.252623e-51	-8.615850e-47
Msa0317300	Msa1286950	-0.835232	1.892087e-56	-8.615850e-47
Msa0317300	Msa1319230	-0.839611	1.428530e-57	-8.615850e-47
Msa0317300	Msa1323260	-0.807244	5.556945e-50	-8.615850e-47
Msa0317300	Msa1343450	-0.804168	2.458436e-49	-8.615850e-47
Msa0317300	Msa1344930	0.815263	1.011285e-51	-8.615850e-47
Msa0317300	Msa1359880	-0.801617	8.276290e-49	-8.615850e-47
Msa0317300	Msa1365850	0.803427	3.503638e-49	-8.615850e-47
Msa0317300	Msa1371800	0.804424	2.174899e-49	-8.615850e-47
Msa0317300	Msa1373560	0.803383	3.577926e-49	-8.615850e-47
Msa0317300	Msa1376310	0.806635	7.476613e-50	-8.615850e-47
Msa0317300	Msa1380740	0.809327	1.999116e-50	-8.615850e-47
Msa0317300	Msa1384410	-0.807649	4.558381e-50	-8.615850e-47
Msa0317300	Msa1392570	-0.802171	6.367733e-49	-8.615850e-47
Msa0317300	Msa1395050	0.845698	3.453439e-59	-8.615850e-47
Msa0317300	Msa1395700	-0.808859	2.518360e-50	-8.615850e-47
Msa0317300	Msa1407670	0.801042	1.085011e-48	-8.615850e-47
Msa0317300	Msa1409060	0.808558	2.920412e-50	-8.615850e-47
Msa0317300	Msa1412740	-0.811505	6.771417e-51	-8.615850e-47
Msa0317300	Msa1423050	0.819666	1.030331e-52	-8.615850e-47
Msa0317300	Msa1430250	0.951945	6.967113e-110	-8.615850e-47
Msa0317300	Msa1449770	0.804736	1.871905e-49	-8.615850e-47
Msa0317300	Msa1459630	-0.800971	1.121875e-48	-8.615850e-47
Msa0317300	Msa1466530	0.827432	1.569932e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0317300	MtrunA17_Chr2g0290461	97.211	251	7	1	251	1	251	4.66e-171	471
Msa0317300	MtrunA17_Chr4g0076261	80.556	72	14	4	75	8	79	8.26e-31	115
Msa0317300	MtrunA17_Chr7g0236071	73.333	75	20	1	75	1	75	3.80e-22	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0317300	AT4G16380.1	42.222	270	113	7	3	251	7	254	4.08e-39	136
Msa0317300	AT4G16380.3	41.636	269	114	7	4	251	15	261	3.75e-38	134
Msa0317300	AT1G51090.1	60.526	76	30	0	1	76	1	76	2.94e-25	98.6
Msa0317300	AT4G16380.2	40.343	233	112	8	32	251	15	233	6.11e-21	88.6
Msa0317300	AT1G49420.1	35.968	253	88	10	3	251	7	189	2.60e-17	77.8

Find 87 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGGCTTCTTTAGCGGGCT+TGG	0.155696	2_4:-14966577	None:intergenic
CTTCTCGGGCTCAGCGGACT+TGG	0.187553	2_4:-14966517	None:intergenic
GACTCAGCTGGTTTGGGCTT+TGG	0.238535	2_4:-14966468	None:intergenic
TTCTCGGACTCAGCTGGTTT+GGG	0.239304	2_4:-14966474	None:intergenic
CTTCTCGGACTCAGCTGGTT+TGG	0.246232	2_4:-14966475	None:intergenic
AGTTGGTGCAGGCTTGTCTT+TGG	0.253073	2_4:-14966712	None:intergenic
AGCAAGAAATTCAACTTTAT+AGG	0.257693	2_4:+14965669	Msa0317300:five_prime_UTR
GCAAGAAATTCAACTTTATA+GGG	0.260805	2_4:+14965670	Msa0317300:five_prime_UTR
TTGCTTTGACATCATACTTT+TGG	0.273180	2_4:-14966341	None:intergenic
GTTGGTGCAGGCTTGTCTTT+GGG	0.283292	2_4:-14966711	None:intergenic
TTCAGCTTCAGGCTTTGGCT+TGG	0.335388	2_4:-14966685	None:intergenic
AGCAGAGCAACAACACAAAA+TGG	0.336414	2_4:-14965753	None:intergenic
TCAGCTTCAGGCTTTGGCTT+GGG	0.337195	2_4:-14966684	None:intergenic
CGCTGAGCCCGAGAAGAAAA+AGG	0.353767	2_4:+14966524	Msa0317300:CDS
TCGGCTTCTTTAGCGGGCTT+GGG	0.362622	2_4:-14966576	None:intergenic
GTTGTAGTCCTGAGAAGATT+AGG	0.368435	2_4:+14966388	Msa0317300:CDS
AGCTGAGTCCGAGAAGAAAA+AGG	0.368826	2_4:+14966482	Msa0317300:CDS
GGCTTCTCGGCTTCTTTAGC+GGG	0.368935	2_4:-14966582	None:intergenic
TGTGGTCCACCATACTGATT+AGG	0.374070	2_4:-14966846	None:intergenic
TGTGCCTCAGATCAGATCTA+TGG	0.388015	2_4:+14965808	Msa0317300:exon
GTCAGCTGTCTTGGGCTTCT+CGG	0.388753	2_4:-14966595	None:intergenic
TGGAGGTGGGCCGTGTTATG+TGG	0.393001	2_4:+14966914	Msa0317300:CDS
GGCATTTGTGGGATCATCAT+AGG	0.405808	2_4:-14966741	None:intergenic
TTCTCGGGCTCAGCGGACTT+GGG	0.416542	2_4:-14966516	None:intergenic
CCCACAAATGCCACCACCTA+TGG	0.417785	2_4:+14966752	Msa0317300:CDS
TTGTAGTCCTGAGAAGATTA+GGG	0.426247	2_4:+14966389	Msa0317300:CDS
AGAAATGCTACAAGAAGGTT+AGG	0.427970	2_4:+14966097	Msa0317300:CDS
CTCAGCGAAGAAAACGCAAC+AGG	0.430429	2_4:+14966954	Msa0317300:CDS
GCCATAGGTGGTGGCATTTG+TGG	0.434523	2_4:-14966753	None:intergenic
TATGGGCCGTATGGCCCAAC+AGG	0.440070	2_4:-14966822	None:intergenic
GATCAGATCTATGGCTGAAA+AGG	0.443352	2_4:+14965817	Msa0317300:exon
CCACCATACTGATTAGGGTA+TGG	0.446251	2_4:-14966840	None:intergenic
GGAACATAACACATCCCAAC+TGG	0.447009	2_4:-14966780	None:intergenic
CCACCATAGCTATCGTATAC+TGG	0.450124	2_4:-14966894	None:intergenic
GGCCCATACCCTAATCAGTA+TGG	0.453969	2_4:+14966837	Msa0317300:CDS
CCATAGGTGGTGGCATTTGT+GGG	0.455573	2_4:-14966752	None:intergenic
CACCTATGGCGGTTCCAGTT+GGG	0.463164	2_4:+14966766	Msa0317300:CDS
TCAGCTGGTTTGGGCTTTGG+CGG	0.463353	2_4:-14966465	None:intergenic
CCACCTATGGCGGTTCCAGT+TGG	0.463360	2_4:+14966765	Msa0317300:CDS
TATCAAGAGTATTGAAATAG+TGG	0.464638	2_4:+14966440	Msa0317300:CDS
TTATTCAGGTGACCATTATG+AGG	0.467676	2_4:+14966052	Msa0317300:intron
GGTAGACCTGTTGGGCCATA+CGG	0.476910	2_4:+14966816	Msa0317300:CDS
TACGATAGCTATGGTGGAGG+TGG	0.483595	2_4:+14966900	Msa0317300:CDS
GGAGGTGGGCCGTGTTATGT+GGG	0.492576	2_4:+14966915	Msa0317300:CDS
AAAATTCATACGTTTCATAG+AGG	0.498578	2_4:+14967054	Msa0317300:three_prime_UTR
AGCTTGTCCCTAATCTTCTC+AGG	0.499784	2_4:-14966396	None:intergenic
CACCATACTGATTAGGGTAT+GGG	0.504883	2_4:-14966839	None:intergenic
ATCAACCTTGAGCCTCATAA+TGG	0.505835	2_4:-14966064	None:intergenic
GGGCTTCTCGGCTTCTTTAG+CGG	0.509630	2_4:-14966583	None:intergenic
GTGGTCCACCATACTGATTA+GGG	0.511313	2_4:-14966845	None:intergenic
GCTACGAGGGTAGACCTGTT+GGG	0.519466	2_4:+14966808	Msa0317300:CDS
TGCTACGAGGGTAGACCTGT+TGG	0.523238	2_4:+14966807	Msa0317300:CDS
ACTGGAACCGCCATAGGTGG+TGG	0.525042	2_4:-14966762	None:intergenic
AGGGACAAGCTTTGTTACAA+AGG	0.533858	2_4:+14966408	Msa0317300:CDS
GTGGGAAATGAAAGTTTATG+AGG	0.536303	2_4:+14967092	Msa0317300:three_prime_UTR
TGATTAGGGTATGGGCCGTA+TGG	0.546802	2_4:-14966831	None:intergenic
AAGCTTTGTTACAAAGGTGG+AGG	0.555529	2_4:+14966414	Msa0317300:CDS
CGCTGAGATATTGATCGCAT+CGG	0.559500	2_4:-14966938	None:intergenic
CCAGTATACGATAGCTATGG+TGG	0.563800	2_4:+14966894	Msa0317300:CDS
ATAGGCATAGCAGTTGGTGC+AGG	0.581534	2_4:-14966723	None:intergenic
GACAAGCTTTGTTACAAAGG+TGG	0.583958	2_4:+14966411	Msa0317300:CDS
ACGATAGCTATGGTGGAGGT+GGG	0.588196	2_4:+14966901	Msa0317300:CDS
TCGCATCGGCCCACATAACA+CGG	0.600233	2_4:-14966924	None:intergenic
GGTGACCATTATGAGGCTCA+AGG	0.605189	2_4:+14966059	Msa0317300:intron
ATCCCAACTGGAACCGCCAT+AGG	0.607318	2_4:-14966768	None:intergenic
TTCAGATAAACCTAAAGATG+CGG	0.608872	2_4:+14966656	Msa0317300:CDS
ATCATCATAGGCATAGCAGT+TGG	0.609583	2_4:-14966729	None:intergenic
GTGTGAGAAATGCTACAAGA+AGG	0.610703	2_4:+14966092	Msa0317300:CDS
GGAAATGAAAGTTTATGAGG+TGG	0.613155	2_4:+14967095	Msa0317300:three_prime_UTR
ACAGGTCTACCCTCGTAGCA+TGG	0.615096	2_4:-14966804	None:intergenic
AGACCAGTATACGATAGCTA+TGG	0.617281	2_4:+14966891	Msa0317300:CDS
GGTGGACCACAATGTTATGA+CGG	0.619668	2_4:+14966858	Msa0317300:CDS
AGATTGTTGATAGACAGTGT+TGG	0.629714	2_4:-14965645	None:intergenic
CCAACTGGAACCGCCATAGG+TGG	0.630035	2_4:-14966765	None:intergenic
AGCTTAATACATAAACATGT+AGG	0.630199	2_4:-14965693	None:intergenic
ACAAATGCCACCACCTATGG+CGG	0.640749	2_4:+14966755	Msa0317300:CDS
AGCAAACATTGTGACAATCA+CGG	0.650689	2_4:+14966359	Msa0317300:CDS
TCAGCCATAGATCTGATCTG+AGG	0.655053	2_4:-14965812	None:intergenic
TAGGCGTGTAAATCAAAAGA+CGG	0.656958	2_4:+14965500	None:intergenic
GTATACGATAGCTATGGTGG+AGG	0.658092	2_4:+14966897	Msa0317300:CDS
TCAGCGAAGAAAACGCAACA+GGG	0.666817	2_4:+14966955	Msa0317300:CDS
CCATACCCTAATCAGTATGG+TGG	0.668874	2_4:+14966840	Msa0317300:CDS
TAGTATCCGTCATAACATTG+TGG	0.672588	2_4:-14966864	None:intergenic
TTATGTTCCACCATGCTACG+AGG	0.686372	2_4:+14966794	Msa0317300:CDS
TATGTTCCACCATGCTACGA+GGG	0.710253	2_4:+14966795	Msa0317300:CDS
GGTCTACCCTCGTAGCATGG+TGG	0.713983	2_4:-14966801	None:intergenic
AAACATTGTGACAATCACGG+TGG	0.804631	2_4:+14966362	Msa0317300:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAACAATTAAAATTATACAT+AGG	-	chr2_4:14966028-14966047	None:intergenic	15.0%
!!!	TTAATTGTTGATTTTTATTC+AGG	+	chr2_4:14966038-14966057	Msa0317300:intron	15.0%
!!!	TATTGTTTTATTAGAATTTG+TGG	+	chr2_4:14966200-14966219	Msa0317300:intron	15.0%
!!!	ATTTTCCAAAAAAATGTAAA+AGG	+	chr2_4:14966256-14966275	Msa0317300:intron	15.0%
!!!	TATATCCTTTTACATTTTTT+TGG	-	chr2_4:14966264-14966283	None:intergenic	15.0%
!!!	TATAAAATTGAGCTAATTTT+TGG	+	chr2_4:14966280-14966299	Msa0317300:intron	15.0%
!!!	ATAAAATTGAGCTAATTTTT+GGG	+	chr2_4:14966281-14966300	Msa0317300:intron	15.0%
!!	AACTTTAGTTATAAAAACAA+AGG	-	chr2_4:14967031-14967050	None:intergenic	15.0%
!!	CAACAATTAAAATTATACAT+AGG	-	chr2_4:14966028-14966047	None:intergenic	15.0%
!!!	TTAATTGTTGATTTTTATTC+AGG	+	chr2_4:14966038-14966057	Msa0317300:intron	15.0%
!!!	TATTGTTTTATTAGAATTTG+TGG	+	chr2_4:14966200-14966219	Msa0317300:intron	15.0%
!!!	ATTTTCCAAAAAAATGTAAA+AGG	+	chr2_4:14966256-14966275	Msa0317300:intron	15.0%
!!!	TATATCCTTTTACATTTTTT+TGG	-	chr2_4:14966264-14966283	None:intergenic	15.0%
!!!	TATAAAATTGAGCTAATTTT+TGG	+	chr2_4:14966280-14966299	Msa0317300:intron	15.0%
!!!	ATAAAATTGAGCTAATTTTT+GGG	+	chr2_4:14966281-14966300	Msa0317300:intron	15.0%
!!	AACTTTAGTTATAAAAACAA+AGG	-	chr2_4:14967031-14967050	None:intergenic	15.0%
!!!	GATCTTCAATTCATTTTAAT+TGG	+	chr2_4:14965924-14965943	Msa0317300:intron	20.0%
!!	AATAAAAGAATAGCATAACA+TGG	-	chr2_4:14965978-14965997	None:intergenic	20.0%
!!!	GATCTTCAATTCATTTTAAT+TGG	+	chr2_4:14965924-14965943	Msa0317300:intron	20.0%
!!	AATAAAAGAATAGCATAACA+TGG	-	chr2_4:14965978-14965997	None:intergenic	20.0%
!	AGCAAGAAATTCAACTTTAT+AGG	+	chr2_4:14965669-14965688	Msa0317300:five_prime_UTR	25.0%
!	GCAAGAAATTCAACTTTATA+GGG	+	chr2_4:14965670-14965689	Msa0317300:five_prime_UTR	25.0%
!	AGCTTAATACATAAACATGT+AGG	-	chr2_4:14965696-14965715	None:intergenic	25.0%
!!!	GGAAAGATTGATAATTTTGA+TGG	-	chr2_4:14965861-14965880	None:intergenic	25.0%
!!	TGACAATTATGTGTTTGTTT+TGG	-	chr2_4:14965889-14965908	None:intergenic	25.0%
!!	ATCATAAATTTGTTTCACCA+TGG	+	chr2_4:14966001-14966020	Msa0317300:intron	25.0%
!	TAAAATTATACATAGGACCA+TGG	-	chr2_4:14966021-14966040	None:intergenic	25.0%
!	ATAGTTAGAAATGAAAGTAG+AGG	-	chr2_4:14966157-14966176	None:intergenic	25.0%
!	TAAAAACACTTGTATGCATT+TGG	+	chr2_4:14966233-14966252	Msa0317300:intron	25.0%
!!!	TATCAAGAGTATTGAAATAG+TGG	+	chr2_4:14966440-14966459	Msa0317300:CDS	25.0%
!!	CAAAAGTAACTTTCTTTTCT+GGG	-	chr2_4:14966620-14966639	None:intergenic	25.0%
!!	ACAAAAGTAACTTTCTTTTC+TGG	-	chr2_4:14966621-14966640	None:intergenic	25.0%
!	AAAATTCATACGTTTCATAG+AGG	+	chr2_4:14967054-14967073	Msa0317300:three_prime_UTR	25.0%
!	TTTCATAGAGGAAAAAAATG+AGG	+	chr2_4:14967066-14967085	Msa0317300:three_prime_UTR	25.0%
!	AGCAAGAAATTCAACTTTAT+AGG	+	chr2_4:14965669-14965688	Msa0317300:five_prime_UTR	25.0%
!	GCAAGAAATTCAACTTTATA+GGG	+	chr2_4:14965670-14965689	Msa0317300:five_prime_UTR	25.0%
!	AGCTTAATACATAAACATGT+AGG	-	chr2_4:14965696-14965715	None:intergenic	25.0%
!!!	GGAAAGATTGATAATTTTGA+TGG	-	chr2_4:14965861-14965880	None:intergenic	25.0%
!!	TGACAATTATGTGTTTGTTT+TGG	-	chr2_4:14965889-14965908	None:intergenic	25.0%
!!	ATCATAAATTTGTTTCACCA+TGG	+	chr2_4:14966001-14966020	Msa0317300:intron	25.0%
!	TAAAATTATACATAGGACCA+TGG	-	chr2_4:14966021-14966040	None:intergenic	25.0%
!	ATAGTTAGAAATGAAAGTAG+AGG	-	chr2_4:14966157-14966176	None:intergenic	25.0%
!	TAAAAACACTTGTATGCATT+TGG	+	chr2_4:14966233-14966252	Msa0317300:intron	25.0%
!!!	TATCAAGAGTATTGAAATAG+TGG	+	chr2_4:14966440-14966459	Msa0317300:CDS	25.0%
!!	CAAAAGTAACTTTCTTTTCT+GGG	-	chr2_4:14966620-14966639	None:intergenic	25.0%
!!	ACAAAAGTAACTTTCTTTTC+TGG	-	chr2_4:14966621-14966640	None:intergenic	25.0%
!	AAAATTCATACGTTTCATAG+AGG	+	chr2_4:14967054-14967073	Msa0317300:three_prime_UTR	25.0%
!	TTTCATAGAGGAAAAAAATG+AGG	+	chr2_4:14967066-14967085	Msa0317300:three_prime_UTR	25.0%
!!!	TATGTGTTTGTTTTGGAGAT+AGG	-	chr2_4:14965882-14965901	None:intergenic	30.0%
!	TTGCTTTGACATCATACTTT+TGG	-	chr2_4:14966344-14966363	None:intergenic	30.0%
	TTCAGATAAACCTAAAGATG+CGG	+	chr2_4:14966656-14966675	Msa0317300:CDS	30.0%
!!!	TATGTGTTTGTTTTGGAGAT+AGG	-	chr2_4:14965882-14965901	None:intergenic	30.0%
!	TTGCTTTGACATCATACTTT+TGG	-	chr2_4:14966344-14966363	None:intergenic	30.0%
	TTCAGATAAACCTAAAGATG+CGG	+	chr2_4:14966656-14966675	Msa0317300:CDS	30.0%
!	GGTTTTTGCTAGAACTGAAA+GGG	-	chr2_4:14965627-14965646	None:intergenic	35.0%
!	TGGTTTTTGCTAGAACTGAA+AGG	-	chr2_4:14965628-14965647	None:intergenic	35.0%
!	AGATTGTTGATAGACAGTGT+TGG	-	chr2_4:14965648-14965667	None:intergenic	35.0%
	TTATTCAGGTGACCATTATG+AGG	+	chr2_4:14966052-14966071	Msa0317300:intron	35.0%
	AGAAATGCTACAAGAAGGTT+AGG	+	chr2_4:14966097-14966116	Msa0317300:CDS	35.0%
	AGCAAACATTGTGACAATCA+CGG	+	chr2_4:14966359-14966378	Msa0317300:CDS	35.0%
	TTGTAGTCCTGAGAAGATTA+GGG	+	chr2_4:14966389-14966408	Msa0317300:CDS	35.0%
	GAACCAGAGAAGAAAAAAGA+AGG	+	chr2_4:14966546-14966565	Msa0317300:CDS	35.0%
	TAGTATCCGTCATAACATTG+TGG	-	chr2_4:14966867-14966886	None:intergenic	35.0%
	CATAGAGGAAAAAAATGAGG+CGG	+	chr2_4:14967069-14967088	Msa0317300:three_prime_UTR	35.0%
	ATAGAGGAAAAAAATGAGGC+GGG	+	chr2_4:14967070-14967089	Msa0317300:three_prime_UTR	35.0%
	GTGGGAAATGAAAGTTTATG+AGG	+	chr2_4:14967092-14967111	Msa0317300:three_prime_UTR	35.0%
	GGAAATGAAAGTTTATGAGG+TGG	+	chr2_4:14967095-14967114	Msa0317300:three_prime_UTR	35.0%
!	GGTTTTTGCTAGAACTGAAA+GGG	-	chr2_4:14965627-14965646	None:intergenic	35.0%
!	TGGTTTTTGCTAGAACTGAA+AGG	-	chr2_4:14965628-14965647	None:intergenic	35.0%
!	AGATTGTTGATAGACAGTGT+TGG	-	chr2_4:14965648-14965667	None:intergenic	35.0%
	TTATTCAGGTGACCATTATG+AGG	+	chr2_4:14966052-14966071	Msa0317300:intron	35.0%
	AGAAATGCTACAAGAAGGTT+AGG	+	chr2_4:14966097-14966116	Msa0317300:CDS	35.0%
	AGCAAACATTGTGACAATCA+CGG	+	chr2_4:14966359-14966378	Msa0317300:CDS	35.0%
	TTGTAGTCCTGAGAAGATTA+GGG	+	chr2_4:14966389-14966408	Msa0317300:CDS	35.0%
	GAACCAGAGAAGAAAAAAGA+AGG	+	chr2_4:14966546-14966565	Msa0317300:CDS	35.0%
	TAGTATCCGTCATAACATTG+TGG	-	chr2_4:14966867-14966886	None:intergenic	35.0%
	CATAGAGGAAAAAAATGAGG+CGG	+	chr2_4:14967069-14967088	Msa0317300:three_prime_UTR	35.0%
	ATAGAGGAAAAAAATGAGGC+GGG	+	chr2_4:14967070-14967089	Msa0317300:three_prime_UTR	35.0%
	GTGGGAAATGAAAGTTTATG+AGG	+	chr2_4:14967092-14967111	Msa0317300:three_prime_UTR	35.0%
	GGAAATGAAAGTTTATGAGG+TGG	+	chr2_4:14967095-14967114	Msa0317300:three_prime_UTR	35.0%
	AGCAGAGCAACAACACAAAA+TGG	-	chr2_4:14965756-14965775	None:intergenic	40.0%
	GATCAGATCTATGGCTGAAA+AGG	+	chr2_4:14965817-14965836	Msa0317300:exon	40.0%
	ATCAACCTTGAGCCTCATAA+TGG	-	chr2_4:14966067-14966086	None:intergenic	40.0%
	GTGTGAGAAATGCTACAAGA+AGG	+	chr2_4:14966092-14966111	Msa0317300:CDS	40.0%
	AAACATTGTGACAATCACGG+TGG	+	chr2_4:14966362-14966381	Msa0317300:CDS	40.0%
	GTTGTAGTCCTGAGAAGATT+AGG	+	chr2_4:14966388-14966407	Msa0317300:CDS	40.0%
!!	AGGGACAAGCTTTGTTACAA+AGG	+	chr2_4:14966408-14966427	Msa0317300:CDS	40.0%
!!	GACAAGCTTTGTTACAAAGG+TGG	+	chr2_4:14966411-14966430	Msa0317300:CDS	40.0%
!!	AAGCTTTGTTACAAAGGTGG+AGG	+	chr2_4:14966414-14966433	Msa0317300:CDS	40.0%
!!	TCTGGTTCCTTTTTCTTCTC+GGG	-	chr2_4:14966534-14966553	None:intergenic	40.0%
!!	CTCTGGTTCCTTTTTCTTCT+CGG	-	chr2_4:14966535-14966554	None:intergenic	40.0%
!!!	TCGCCTTCTTTTTTCTTCTC+TGG	-	chr2_4:14966552-14966571	None:intergenic	40.0%
	ATCATCATAGGCATAGCAGT+TGG	-	chr2_4:14966732-14966751	None:intergenic	40.0%
!	CACCATACTGATTAGGGTAT+GGG	-	chr2_4:14966842-14966861	None:intergenic	40.0%
	AGACCAGTATACGATAGCTA+TGG	+	chr2_4:14966891-14966910	Msa0317300:CDS	40.0%
	AGCAGAGCAACAACACAAAA+TGG	-	chr2_4:14965756-14965775	None:intergenic	40.0%
	GATCAGATCTATGGCTGAAA+AGG	+	chr2_4:14965817-14965836	Msa0317300:exon	40.0%
	ATCAACCTTGAGCCTCATAA+TGG	-	chr2_4:14966067-14966086	None:intergenic	40.0%
	GTGTGAGAAATGCTACAAGA+AGG	+	chr2_4:14966092-14966111	Msa0317300:CDS	40.0%
	AAACATTGTGACAATCACGG+TGG	+	chr2_4:14966362-14966381	Msa0317300:CDS	40.0%
	GTTGTAGTCCTGAGAAGATT+AGG	+	chr2_4:14966388-14966407	Msa0317300:CDS	40.0%
!!	AGGGACAAGCTTTGTTACAA+AGG	+	chr2_4:14966408-14966427	Msa0317300:CDS	40.0%
!!	GACAAGCTTTGTTACAAAGG+TGG	+	chr2_4:14966411-14966430	Msa0317300:CDS	40.0%
!!	AAGCTTTGTTACAAAGGTGG+AGG	+	chr2_4:14966414-14966433	Msa0317300:CDS	40.0%
!!	TCTGGTTCCTTTTTCTTCTC+GGG	-	chr2_4:14966534-14966553	None:intergenic	40.0%
!!	CTCTGGTTCCTTTTTCTTCT+CGG	-	chr2_4:14966535-14966554	None:intergenic	40.0%
!!!	TCGCCTTCTTTTTTCTTCTC+TGG	-	chr2_4:14966552-14966571	None:intergenic	40.0%
	ATCATCATAGGCATAGCAGT+TGG	-	chr2_4:14966732-14966751	None:intergenic	40.0%
!	CACCATACTGATTAGGGTAT+GGG	-	chr2_4:14966842-14966861	None:intergenic	40.0%
	AGACCAGTATACGATAGCTA+TGG	+	chr2_4:14966891-14966910	Msa0317300:CDS	40.0%
	TGTGCCTCAGATCAGATCTA+TGG	+	chr2_4:14965808-14965827	Msa0317300:exon	45.0%
	TCAGCCATAGATCTGATCTG+AGG	-	chr2_4:14965815-14965834	None:intergenic	45.0%
	TAGAGGTGATGAACTCACGA+GGG	-	chr2_4:14966140-14966159	None:intergenic	45.0%
	AGCTTGTCCCTAATCTTCTC+AGG	-	chr2_4:14966399-14966418	None:intergenic	45.0%
!	TTTTTCTTCTCGGACTCAGC+TGG	-	chr2_4:14966483-14966502	None:intergenic	45.0%
	AGCTGAGTCCGAGAAGAAAA+AGG	+	chr2_4:14966482-14966501	Msa0317300:CDS	45.0%
	GAGTCCGAGAAGAAAAAGGA+AGG	+	chr2_4:14966486-14966505	Msa0317300:CDS	45.0%
!	CTCGCCTTCCTTTTTCTTCT+CGG	-	chr2_4:14966493-14966512	None:intergenic	45.0%
!	TTTTCTGGGTCAGCTGTCTT+GGG	-	chr2_4:14966606-14966625	None:intergenic	45.0%
!	TTGGGCTTTTCCGCATCTTT+AGG	-	chr2_4:14966669-14966688	None:intergenic	45.0%
!	TCTTTGGGCTTTTCAGCTTC+AGG	-	chr2_4:14966699-14966718	None:intergenic	45.0%
	GGCATTTGTGGGATCATCAT+AGG	-	chr2_4:14966744-14966763	None:intergenic	45.0%
	GGAACATAACACATCCCAAC+TGG	-	chr2_4:14966783-14966802	None:intergenic	45.0%
	TTATGTTCCACCATGCTACG+AGG	+	chr2_4:14966794-14966813	Msa0317300:CDS	45.0%
	TATGTTCCACCATGCTACGA+GGG	+	chr2_4:14966795-14966814	Msa0317300:CDS	45.0%
!	CCACCATACTGATTAGGGTA+TGG	-	chr2_4:14966843-14966862	None:intergenic	45.0%
	CCATACCCTAATCAGTATGG+TGG	+	chr2_4:14966840-14966859	Msa0317300:CDS	45.0%
!	GTGGTCCACCATACTGATTA+GGG	-	chr2_4:14966848-14966867	None:intergenic	45.0%
!	TGTGGTCCACCATACTGATT+AGG	-	chr2_4:14966849-14966868	None:intergenic	45.0%
	GGTGGACCACAATGTTATGA+CGG	+	chr2_4:14966858-14966877	Msa0317300:CDS	45.0%
	CCACCATAGCTATCGTATAC+TGG	-	chr2_4:14966897-14966916	None:intergenic	45.0%
	CCAGTATACGATAGCTATGG+TGG	+	chr2_4:14966894-14966913	Msa0317300:CDS	45.0%
	GTATACGATAGCTATGGTGG+AGG	+	chr2_4:14966897-14966916	Msa0317300:CDS	45.0%
	CGCTGAGATATTGATCGCAT+CGG	-	chr2_4:14966941-14966960	None:intergenic	45.0%
	TCAGCGAAGAAAACGCAACA+GGG	+	chr2_4:14966955-14966974	Msa0317300:CDS	45.0%
	AGGAAAAAAATGAGGCGGGT+GGG	+	chr2_4:14967074-14967093	Msa0317300:three_prime_UTR	45.0%
	TGTGCCTCAGATCAGATCTA+TGG	+	chr2_4:14965808-14965827	Msa0317300:exon	45.0%
	TCAGCCATAGATCTGATCTG+AGG	-	chr2_4:14965815-14965834	None:intergenic	45.0%
	TAGAGGTGATGAACTCACGA+GGG	-	chr2_4:14966140-14966159	None:intergenic	45.0%
	AGCTTGTCCCTAATCTTCTC+AGG	-	chr2_4:14966399-14966418	None:intergenic	45.0%
!	TTTTTCTTCTCGGACTCAGC+TGG	-	chr2_4:14966483-14966502	None:intergenic	45.0%
	AGCTGAGTCCGAGAAGAAAA+AGG	+	chr2_4:14966482-14966501	Msa0317300:CDS	45.0%
	GAGTCCGAGAAGAAAAAGGA+AGG	+	chr2_4:14966486-14966505	Msa0317300:CDS	45.0%
!	CTCGCCTTCCTTTTTCTTCT+CGG	-	chr2_4:14966493-14966512	None:intergenic	45.0%
!	TTTTCTGGGTCAGCTGTCTT+GGG	-	chr2_4:14966606-14966625	None:intergenic	45.0%
!	TTGGGCTTTTCCGCATCTTT+AGG	-	chr2_4:14966669-14966688	None:intergenic	45.0%
!	TCTTTGGGCTTTTCAGCTTC+AGG	-	chr2_4:14966699-14966718	None:intergenic	45.0%
	GGCATTTGTGGGATCATCAT+AGG	-	chr2_4:14966744-14966763	None:intergenic	45.0%
	GGAACATAACACATCCCAAC+TGG	-	chr2_4:14966783-14966802	None:intergenic	45.0%
	TTATGTTCCACCATGCTACG+AGG	+	chr2_4:14966794-14966813	Msa0317300:CDS	45.0%
	TATGTTCCACCATGCTACGA+GGG	+	chr2_4:14966795-14966814	Msa0317300:CDS	45.0%
!	CCACCATACTGATTAGGGTA+TGG	-	chr2_4:14966843-14966862	None:intergenic	45.0%
	CCATACCCTAATCAGTATGG+TGG	+	chr2_4:14966840-14966859	Msa0317300:CDS	45.0%
!	GTGGTCCACCATACTGATTA+GGG	-	chr2_4:14966848-14966867	None:intergenic	45.0%
!	TGTGGTCCACCATACTGATT+AGG	-	chr2_4:14966849-14966868	None:intergenic	45.0%
	GGTGGACCACAATGTTATGA+CGG	+	chr2_4:14966858-14966877	Msa0317300:CDS	45.0%
	CCACCATAGCTATCGTATAC+TGG	-	chr2_4:14966897-14966916	None:intergenic	45.0%
	CCAGTATACGATAGCTATGG+TGG	+	chr2_4:14966894-14966913	Msa0317300:CDS	45.0%
	GTATACGATAGCTATGGTGG+AGG	+	chr2_4:14966897-14966916	Msa0317300:CDS	45.0%
	CGCTGAGATATTGATCGCAT+CGG	-	chr2_4:14966941-14966960	None:intergenic	45.0%
	TCAGCGAAGAAAACGCAACA+GGG	+	chr2_4:14966955-14966974	Msa0317300:CDS	45.0%
	AGGAAAAAAATGAGGCGGGT+GGG	+	chr2_4:14967074-14967093	Msa0317300:three_prime_UTR	45.0%
	GGTGACCATTATGAGGCTCA+AGG	+	chr2_4:14966059-14966078	Msa0317300:intron	50.0%
	GTAGAGGTGATGAACTCACG+AGG	-	chr2_4:14966141-14966160	None:intergenic	50.0%
!	TTCTCGGACTCAGCTGGTTT+GGG	-	chr2_4:14966477-14966496	None:intergenic	50.0%
!	CTTTTTCTTCTCGGGCTCAG+CGG	-	chr2_4:14966526-14966545	None:intergenic	50.0%
!	CTTTTCTGGGTCAGCTGTCT+TGG	-	chr2_4:14966607-14966626	None:intergenic	50.0%
!	TCAGCTTCAGGCTTTGGCTT+GGG	-	chr2_4:14966687-14966706	None:intergenic	50.0%
!	TTCAGCTTCAGGCTTTGGCT+TGG	-	chr2_4:14966688-14966707	None:intergenic	50.0%
!	GGCTTTTCAGCTTCAGGCTT+TGG	-	chr2_4:14966693-14966712	None:intergenic	50.0%
	GTTGGTGCAGGCTTGTCTTT+GGG	-	chr2_4:14966714-14966733	None:intergenic	50.0%
!	AGTTGGTGCAGGCTTGTCTT+TGG	-	chr2_4:14966715-14966734	None:intergenic	50.0%
!	ATAGGCATAGCAGTTGGTGC+AGG	-	chr2_4:14966726-14966745	None:intergenic	50.0%
!	CCATAGGTGGTGGCATTTGT+GGG	-	chr2_4:14966755-14966774	None:intergenic	50.0%
	ACAAATGCCACCACCTATGG+CGG	+	chr2_4:14966755-14966774	Msa0317300:CDS	50.0%
	TGATTAGGGTATGGGCCGTA+TGG	-	chr2_4:14966834-14966853	None:intergenic	50.0%
	GGCCCATACCCTAATCAGTA+TGG	+	chr2_4:14966837-14966856	Msa0317300:CDS	50.0%
!	TACGATAGCTATGGTGGAGG+TGG	+	chr2_4:14966900-14966919	Msa0317300:CDS	50.0%
!	ACGATAGCTATGGTGGAGGT+GGG	+	chr2_4:14966901-14966920	Msa0317300:CDS	50.0%
	CTCAGCGAAGAAAACGCAAC+AGG	+	chr2_4:14966954-14966973	Msa0317300:CDS	50.0%
	GAGGAAAAAAATGAGGCGGG+TGG	+	chr2_4:14967073-14967092	Msa0317300:three_prime_UTR	50.0%
	GGTGACCATTATGAGGCTCA+AGG	+	chr2_4:14966059-14966078	Msa0317300:intron	50.0%
	GTAGAGGTGATGAACTCACG+AGG	-	chr2_4:14966141-14966160	None:intergenic	50.0%
!	TTCTCGGACTCAGCTGGTTT+GGG	-	chr2_4:14966477-14966496	None:intergenic	50.0%
!	CTTTTTCTTCTCGGGCTCAG+CGG	-	chr2_4:14966526-14966545	None:intergenic	50.0%
!	CTTTTCTGGGTCAGCTGTCT+TGG	-	chr2_4:14966607-14966626	None:intergenic	50.0%
!	TCAGCTTCAGGCTTTGGCTT+GGG	-	chr2_4:14966687-14966706	None:intergenic	50.0%
!	TTCAGCTTCAGGCTTTGGCT+TGG	-	chr2_4:14966688-14966707	None:intergenic	50.0%
!	GGCTTTTCAGCTTCAGGCTT+TGG	-	chr2_4:14966693-14966712	None:intergenic	50.0%
	GTTGGTGCAGGCTTGTCTTT+GGG	-	chr2_4:14966714-14966733	None:intergenic	50.0%
!	AGTTGGTGCAGGCTTGTCTT+TGG	-	chr2_4:14966715-14966734	None:intergenic	50.0%
!	ATAGGCATAGCAGTTGGTGC+AGG	-	chr2_4:14966726-14966745	None:intergenic	50.0%
!	CCATAGGTGGTGGCATTTGT+GGG	-	chr2_4:14966755-14966774	None:intergenic	50.0%
	ACAAATGCCACCACCTATGG+CGG	+	chr2_4:14966755-14966774	Msa0317300:CDS	50.0%
	TGATTAGGGTATGGGCCGTA+TGG	-	chr2_4:14966834-14966853	None:intergenic	50.0%
	GGCCCATACCCTAATCAGTA+TGG	+	chr2_4:14966837-14966856	Msa0317300:CDS	50.0%
!	TACGATAGCTATGGTGGAGG+TGG	+	chr2_4:14966900-14966919	Msa0317300:CDS	50.0%
!	ACGATAGCTATGGTGGAGGT+GGG	+	chr2_4:14966901-14966920	Msa0317300:CDS	50.0%
	CTCAGCGAAGAAAACGCAAC+AGG	+	chr2_4:14966954-14966973	Msa0317300:CDS	50.0%
	GAGGAAAAAAATGAGGCGGG+TGG	+	chr2_4:14967073-14967092	Msa0317300:three_prime_UTR	50.0%
!!	TCAGCTGGTTTGGGCTTTGG+CGG	-	chr2_4:14966468-14966487	None:intergenic	55.0%
!!	GACTCAGCTGGTTTGGGCTT+TGG	-	chr2_4:14966471-14966490	None:intergenic	55.0%
	CTTCTCGGACTCAGCTGGTT+TGG	-	chr2_4:14966478-14966497	None:intergenic	55.0%
	CGCTGAGCCCGAGAAGAAAA+AGG	+	chr2_4:14966524-14966543	Msa0317300:CDS	55.0%
!!	TCGGCTTCTTTAGCGGGCTT+GGG	-	chr2_4:14966579-14966598	None:intergenic	55.0%
	GGCTTCTCGGCTTCTTTAGC+GGG	-	chr2_4:14966585-14966604	None:intergenic	55.0%
	GGGCTTCTCGGCTTCTTTAG+CGG	-	chr2_4:14966586-14966605	None:intergenic	55.0%
	GTCAGCTGTCTTGGGCTTCT+CGG	-	chr2_4:14966598-14966617	None:intergenic	55.0%
	CCCACAAATGCCACCACCTA+TGG	+	chr2_4:14966752-14966771	Msa0317300:CDS	55.0%
!	GCCATAGGTGGTGGCATTTG+TGG	-	chr2_4:14966756-14966775	None:intergenic	55.0%
	CACCTATGGCGGTTCCAGTT+GGG	+	chr2_4:14966766-14966785	Msa0317300:CDS	55.0%
	ATCCCAACTGGAACCGCCAT+AGG	-	chr2_4:14966771-14966790	None:intergenic	55.0%
	ACAGGTCTACCCTCGTAGCA+TGG	-	chr2_4:14966807-14966826	None:intergenic	55.0%
	TGCTACGAGGGTAGACCTGT+TGG	+	chr2_4:14966807-14966826	Msa0317300:CDS	55.0%
	GCTACGAGGGTAGACCTGTT+GGG	+	chr2_4:14966808-14966827	Msa0317300:CDS	55.0%
	GGTAGACCTGTTGGGCCATA+CGG	+	chr2_4:14966816-14966835	Msa0317300:CDS	55.0%
!	TCGCATCGGCCCACATAACA+CGG	-	chr2_4:14966927-14966946	None:intergenic	55.0%
!!	TCAGCTGGTTTGGGCTTTGG+CGG	-	chr2_4:14966468-14966487	None:intergenic	55.0%
!!	GACTCAGCTGGTTTGGGCTT+TGG	-	chr2_4:14966471-14966490	None:intergenic	55.0%
	CTTCTCGGACTCAGCTGGTT+TGG	-	chr2_4:14966478-14966497	None:intergenic	55.0%
	CGCTGAGCCCGAGAAGAAAA+AGG	+	chr2_4:14966524-14966543	Msa0317300:CDS	55.0%
!!	TCGGCTTCTTTAGCGGGCTT+GGG	-	chr2_4:14966579-14966598	None:intergenic	55.0%
	GGCTTCTCGGCTTCTTTAGC+GGG	-	chr2_4:14966585-14966604	None:intergenic	55.0%
	GGGCTTCTCGGCTTCTTTAG+CGG	-	chr2_4:14966586-14966605	None:intergenic	55.0%
	GTCAGCTGTCTTGGGCTTCT+CGG	-	chr2_4:14966598-14966617	None:intergenic	55.0%
	CCCACAAATGCCACCACCTA+TGG	+	chr2_4:14966752-14966771	Msa0317300:CDS	55.0%
!	GCCATAGGTGGTGGCATTTG+TGG	-	chr2_4:14966756-14966775	None:intergenic	55.0%
	CACCTATGGCGGTTCCAGTT+GGG	+	chr2_4:14966766-14966785	Msa0317300:CDS	55.0%
	ATCCCAACTGGAACCGCCAT+AGG	-	chr2_4:14966771-14966790	None:intergenic	55.0%
	ACAGGTCTACCCTCGTAGCA+TGG	-	chr2_4:14966807-14966826	None:intergenic	55.0%
	TGCTACGAGGGTAGACCTGT+TGG	+	chr2_4:14966807-14966826	Msa0317300:CDS	55.0%
	GCTACGAGGGTAGACCTGTT+GGG	+	chr2_4:14966808-14966827	Msa0317300:CDS	55.0%
	GGTAGACCTGTTGGGCCATA+CGG	+	chr2_4:14966816-14966835	Msa0317300:CDS	55.0%
!	TCGCATCGGCCCACATAACA+CGG	-	chr2_4:14966927-14966946	None:intergenic	55.0%
!	TTCTCGGGCTCAGCGGACTT+GGG	-	chr2_4:14966519-14966538	None:intergenic	60.0%
!!	CTCGGCTTCTTTAGCGGGCT+TGG	-	chr2_4:14966580-14966599	None:intergenic	60.0%
	ACTGGAACCGCCATAGGTGG+TGG	-	chr2_4:14966765-14966784	None:intergenic	60.0%
	CCAACTGGAACCGCCATAGG+TGG	-	chr2_4:14966768-14966787	None:intergenic	60.0%
	CCACCTATGGCGGTTCCAGT+TGG	+	chr2_4:14966765-14966784	Msa0317300:CDS	60.0%
	GGTCTACCCTCGTAGCATGG+TGG	-	chr2_4:14966804-14966823	None:intergenic	60.0%
	TATGGGCCGTATGGCCCAAC+AGG	-	chr2_4:14966825-14966844	None:intergenic	60.0%
	TGGAGGTGGGCCGTGTTATG+TGG	+	chr2_4:14966914-14966933	Msa0317300:CDS	60.0%
	GGAGGTGGGCCGTGTTATGT+GGG	+	chr2_4:14966915-14966934	Msa0317300:CDS	60.0%
!	TTCTCGGGCTCAGCGGACTT+GGG	-	chr2_4:14966519-14966538	None:intergenic	60.0%
!!	CTCGGCTTCTTTAGCGGGCT+TGG	-	chr2_4:14966580-14966599	None:intergenic	60.0%
	ACTGGAACCGCCATAGGTGG+TGG	-	chr2_4:14966765-14966784	None:intergenic	60.0%
	CCAACTGGAACCGCCATAGG+TGG	-	chr2_4:14966768-14966787	None:intergenic	60.0%
	CCACCTATGGCGGTTCCAGT+TGG	+	chr2_4:14966765-14966784	Msa0317300:CDS	60.0%
	GGTCTACCCTCGTAGCATGG+TGG	-	chr2_4:14966804-14966823	None:intergenic	60.0%
	TATGGGCCGTATGGCCCAAC+AGG	-	chr2_4:14966825-14966844	None:intergenic	60.0%
	TGGAGGTGGGCCGTGTTATG+TGG	+	chr2_4:14966914-14966933	Msa0317300:CDS	60.0%
	GGAGGTGGGCCGTGTTATGT+GGG	+	chr2_4:14966915-14966934	Msa0317300:CDS	60.0%
!	CTTCTCGGGCTCAGCGGACT+TGG	-	chr2_4:14966520-14966539	None:intergenic	65.0%
!	CTTCTCGGGCTCAGCGGACT+TGG	-	chr2_4:14966520-14966539	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	14965506	14967189	14965506	ID=Msa0317300;Name=Msa0317300
chr2_4	mRNA	14965506	14967189	14965506	ID=Msa0317300-mRNA-1;Parent=Msa0317300;Name=Msa0317300-mRNA-1;_AED=0.10;_eAED=0.10;_QI=321\|1\|1\|1\|1\|1\|3\|197\|251
chr2_4	exon	14965506	14965838	14965506	ID=Msa0317300-mRNA-1:exon:5595;Parent=Msa0317300-mRNA-1
chr2_4	exon	14966060	14966141	14966060	ID=Msa0317300-mRNA-1:exon:5596;Parent=Msa0317300-mRNA-1
chr2_4	exon	14966331	14967189	14966331	ID=Msa0317300-mRNA-1:exon:5597;Parent=Msa0317300-mRNA-1
chr2_4	five_prime_UTR	14965506	14965826	14965506	ID=Msa0317300-mRNA-1:five_prime_utr;Parent=Msa0317300-mRNA-1
chr2_4	CDS	14965827	14965838	14965827	ID=Msa0317300-mRNA-1:cds;Parent=Msa0317300-mRNA-1
chr2_4	CDS	14966060	14966141	14966060	ID=Msa0317300-mRNA-1:cds;Parent=Msa0317300-mRNA-1
chr2_4	CDS	14966331	14966992	14966331	ID=Msa0317300-mRNA-1:cds;Parent=Msa0317300-mRNA-1
chr2_4	three_prime_UTR	14966993	14967189	14966993	ID=Msa0317300-mRNA-1:three_prime_utr;Parent=Msa0317300-mRNA-1

Gene Sequence

>Msa0317300

ATGGCTGAAAAGGTGACCATTATGAGGCTCAAGGTTGATCTTGAGTGTGAGAAATGCTACAAGAAGGTTAGGAAGCTTCTCAACAAGTACCCTCAAATTCGAGACCAAAAGTATGATGTCAAAGCAAACATTGTGACAATCACGGTGGTATGTTGTAGTCCTGAGAAGATTAGGGACAAGCTTTGTTACAAAGGTGGAGGTTCTATCAAGAGTATTGAAATAGTGGATCCGCCAAAGCCCAAACCAGCTGAGTCCGAGAAGAAAAAGGAAGGCGAGAAGCCCAAGTCCGCTGAGCCCGAGAAGAAAAAGGAACCAGAGAAGAAAAAAGAAGGCGAGAAGCCCAAGCCCGCTAAAGAAGCCGAGAAGCCCAAGACAGCTGACCCAGAAAAGAAAGTTACTTTTGTTTCTGTAGTCAAAGATTCAGATAAACCTAAAGATGCGGAAAAGCCCAAGCCAAAGCCTGAAGCTGAAAAGCCCAAAGACAAGCCTGCACCAACTGCTATGCCTATGATGATCCCACAAATGCCACCACCTATGGCGGTTCCAGTTGGGATGTGTTATGTTCCACCATGCTACGAGGGTAGACCTGTTGGGCCATACGGCCCATACCCTAATCAGTATGGTGGACCACAATGTTATGACGGATACTATGCAAGACCAGTATACGATAGCTATGGTGGAGGTGGGCCGTGTTATGTGGGCCGATGCGATCAATATCTCAGCGAAGAAAACGCAACAGGGTGCACAATTATGTGA

Protein sequence

>Msa0317300

MAEKVTIMRLKVDLECEKCYKKVRKLLNKYPQIRDQKYDVKANIVTITVVCCSPEKIRDKLCYKGGGSIKSIEIVDPPKPKPAESEKKKEGEKPKSAEPEKKKEPEKKKEGEKPKPAKEAEKPKTADPEKKVTFVSVVKDSDKPKDAEKPKPKPEAEKPKDKPAPTAMPMMIPQMPPPMAVPVGMCYVPPCYEGRPVGPYGPYPNQYGGPQCYDGYYARPVYDSYGGGGPCYVGRCDQYLSEENATGCTIM*