AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1380490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380490 MtrunA17_Chr4g0056371 95.455 132 5 1 1 131 1 132 4.47e-86 246
Msa1380490 MtrunA17_Chr4g0060391 92.366 131 2 1 1 131 1 123 7.71e-81 233
Msa1380490 MtrunA17_Chr2g0290521 54.688 128 52 3 4 131 6 127 2.78e-38 125
Msa1380490 MtrunA17_Chr4g0056391 54.015 137 55 2 3 131 7 143 7.15e-38 125
Msa1380490 MtrunA17_Chr4g0056411 47.853 163 49 3 3 130 7 168 2.80e-37 124
Msa1380490 MtrunA17_Chr1g0149481 55.833 120 39 3 26 131 45 164 3.53e-36 121
Msa1380490 MtrunA17_Chr1g0149491 63.855 83 30 0 49 131 69 151 2.75e-34 116
Msa1380490 MtrunA17_Chr1g0149471 66.265 83 28 0 49 131 128 210 4.23e-34 117
Msa1380490 MtrunA17_Chr3g0137521 68.675 83 25 1 49 130 47 129 7.06e-34 114
Msa1380490 MtrunA17_Chr4g0056401 45.802 131 68 2 2 131 6 134 7.72e-30 104
Msa1380490 MtrunA17_Chr3g0137531 60.714 84 32 1 49 131 46 129 2.88e-24 90.5
Msa1380490 MtrunA17_Chr4g0056381 53.571 84 37 2 48 130 104 186 2.71e-21 84.3
Msa1380490 MtrunA17_Chr5g0399081 40.000 105 45 3 43 130 73 176 6.22e-20 80.5
Msa1380490 MtrunA17_Chr2g0290571 46.835 79 40 1 52 130 122 198 2.16e-17 74.3
Msa1380490 MtrunA17_Chr2g0290561 46.835 79 40 1 52 130 118 194 1.59e-16 72.0
Msa1380490 MtrunA17_Chr2g0290531 46.053 76 36 2 58 131 188 260 1.47e-15 70.5
Msa1380490 MtrunA17_Chr3g0120411 42.857 84 46 2 48 130 253 335 9.46e-13 63.5
Msa1380490 MtrunA17_Chr4g0064361 48.235 85 42 2 48 131 150 233 4.67e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380490 AT2G45180.1 62.222 135 46 2 1 131 1 134 4.52e-47 148
Msa1380490 AT5G46890.1 64.341 129 41 4 3 131 4 127 3.96e-42 135
Msa1380490 AT4G12520.1 52.941 136 52 3 1 131 1 129 1.88e-38 126
Msa1380490 AT4G12510.1 52.941 136 52 3 1 131 1 129 1.88e-38 126
Msa1380490 AT5G46900.1 62.791 129 43 3 3 131 4 127 4.43e-38 125
Msa1380490 AT1G62510.1 73.494 83 21 1 49 130 67 149 1.38e-36 122
Msa1380490 AT4G00165.1 52.846 123 43 3 12 130 17 128 3.72e-36 120
Msa1380490 AT4G00165.2 52.846 123 43 3 12 130 17 128 3.72e-36 120
Msa1380490 AT4G12480.1 40.476 168 63 3 1 131 1 168 6.60e-34 116
Msa1380490 AT4G12490.1 41.530 183 54 4 1 131 1 182 9.08e-34 116
Msa1380490 AT4G12550.1 49.219 128 41 2 5 131 7 111 1.04e-33 114
Msa1380490 AT1G12090.1 72.840 81 21 1 51 130 57 137 1.39e-33 114
Msa1380490 AT4G22460.1 57.692 104 41 3 26 128 29 130 2.38e-33 114
Msa1380490 AT4G12545.1 48.462 130 40 2 3 131 5 108 2.17e-32 110
Msa1380490 AT4G12470.1 42.236 161 63 3 1 131 1 161 8.16e-32 110
Msa1380490 AT4G12500.1 61.905 84 31 1 49 131 94 177 1.30e-31 110
Msa1380490 AT1G12100.1 46.094 128 62 3 6 131 10 132 9.23e-30 104
Msa1380490 AT4G12530.1 55.422 83 37 0 48 130 33 115 3.00e-27 97.8
Msa1380490 AT3G22142.1 52.381 84 38 2 48 130 1396 1478 1.76e-19 83.6
Msa1380490 AT2G10940.2 49.412 85 41 2 48 131 207 290 1.34e-18 79.7
Msa1380490 AT2G10940.1 49.412 85 41 2 48 131 207 290 1.34e-18 79.7
Msa1380490 AT3G22120.2 54.118 85 36 3 48 130 266 349 4.16e-18 79.0
Msa1380490 AT1G62500.1 47.126 87 44 2 45 130 208 293 4.67e-18 78.2
Msa1380490 AT3G22120.1 54.118 85 36 3 48 130 249 332 8.26e-18 77.8
Msa1380490 AT4G15160.2 51.163 86 38 4 48 130 98 182 3.41e-17 74.3
Msa1380490 AT4G15160.1 51.163 86 38 4 48 130 180 264 1.15e-16 74.3
Msa1380490 AT4G22490.1 32.584 89 58 1 42 130 30 116 3.04e-13 62.0
Msa1380490 AT4G22520.1 29.231 130 73 3 1 130 7 117 5.27e-12 58.9

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTAATGGGAAGATTA+AGG 0.239655 tig0020375:-5419 None:intergenic
AAGAACCAAGCTCAAATTAA+TGG 0.256221 tig0020375:-5430 None:intergenic
TGGTGTGTGTGCTGATGTAT+TGG 0.312025 tig0020375:+5269 Msa1380490:CDS
GCTCTTAAGGCTAATATCTT+AGG 0.323048 tig0020375:+5390 Msa1380490:CDS
GTGCCTTTGTACTGCTCTTA+AGG 0.328363 tig0020375:+5377 Msa1380490:CDS
CCTAGAGACACAATTAAGTT+TGG 0.348121 tig0020375:+5249 Msa1380490:CDS
GGTGTGTGTGCTGATGTATT+GGG 0.364523 tig0020375:+5270 Msa1380490:CDS
CCATGCTGCTCTCTCATTGA+TGG 0.378656 tig0020375:+5330 Msa1380490:CDS
AGGACAAGTTGGATATTTAG+AGG 0.384493 tig0020375:-5229 None:intergenic
AAGGTGGATGTGGGTGTTTG+TGG 0.411134 tig0020375:-5206 None:intergenic
AGAGAGCAGCATGGTGTCTT+TGG 0.413985 tig0020375:-5321 None:intergenic
AGAACCAAGCTCAAATTAAT+GGG 0.417409 tig0020375:-5429 None:intergenic
TAAGCACACACGAAACCCTT+TGG 0.418402 tig0020375:-5474 None:intergenic
TGTGATCTTTGTGAGGTGGT+GGG 0.421624 tig0020375:-5182 None:intergenic
GTTCTTAACTATTGTGGAAA+GGG 0.421891 tig0020375:+5447 Msa1380490:CDS
CTGTGATCTTTGTGAGGTGG+TGG 0.426802 tig0020375:-5183 None:intergenic
CAAGTTGGATATTTAGAGGA+AGG 0.430718 tig0020375:-5225 None:intergenic
AGCTTGGTTCTTAACTATTG+TGG 0.458633 tig0020375:+5441 Msa1380490:CDS
GTGATCTTTGTGAGGTGGTG+GGG 0.458860 tig0020375:-5181 None:intergenic
TGATCTTTGTGAGGTGGTGG+GGG 0.471644 tig0020375:-5180 None:intergenic
GAGCAGCATGGTGTCTTTGG+TGG 0.477521 tig0020375:-5318 None:intergenic
TATTTAGAGGAAGGTGGATG+TGG 0.477977 tig0020375:-5216 None:intergenic
GCAAAGGAGCATAGCAACCT+TGG 0.496091 tig0020375:-5109 None:intergenic
ATCATTCCAAATGGCTTCCA+AGG 0.499837 tig0020375:+5092 None:intergenic
TCTTCCCATTAATTTGAGCT+TGG 0.503356 tig0020375:+5425 Msa1380490:CDS
ATTGTGTCTCTAGGACAAGT+TGG 0.512609 tig0020375:-5240 None:intergenic
GGTTTGATTAATGTTGAGCT+TGG 0.521860 tig0020375:+5291 Msa1380490:CDS
GGTTCTTAACTATTGTGGAA+AGG 0.535979 tig0020375:+5446 Msa1380490:CDS
CCAAACTTAATTGTGTCTCT+AGG 0.547203 tig0020375:-5249 None:intergenic
GTTGGATATTTAGAGGAAGG+TGG 0.548060 tig0020375:-5222 None:intergenic
TTGTGGAAAGGGAGTTCCAA+AGG 0.553018 tig0020375:+5458 Msa1380490:CDS
ATTTAGAGGAAGGTGGATGT+GGG 0.563461 tig0020375:-5215 None:intergenic
TGTGGAAAGGGAGTTCCAAA+GGG 0.568343 tig0020375:+5459 Msa1380490:CDS
TGGCTGTGATCTTTGTGAGG+TGG 0.576896 tig0020375:-5186 None:intergenic
AAAATAGGATGTTGAGGCAA+AGG 0.587552 tig0020375:-5125 None:intergenic
TTGTGGCTGTGATCTTTGTG+AGG 0.617514 tig0020375:-5189 None:intergenic
TAGCCTTAAGAGCAGTACAA+AGG 0.662253 tig0020375:-5380 None:intergenic
CCATCAATGAGAGAGCAGCA+TGG 0.704306 tig0020375:-5330 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAAATTAATGGGAAGATTA+AGG - tig0020375:5422-5441 None:intergenic 25.0%
AGAACCAAGCTCAAATTAAT+GGG - tig0020375:5432-5451 None:intergenic 30.0%
AAGAACCAAGCTCAAATTAA+TGG - tig0020375:5433-5452 None:intergenic 30.0%
GTTCTTAACTATTGTGGAAA+GGG + tig0020375:5447-5466 Msa1380490:CDS 30.0%
AAAATAGGATGTTGAGGCAA+AGG - tig0020375:5128-5147 None:intergenic 35.0%
CAGTGAAAAATAGGATGTTG+AGG - tig0020375:5134-5153 None:intergenic 35.0%
!! CAACATCCTATTTTTCACTG+TGG + tig0020375:5134-5153 Msa1380490:CDS 35.0%
AGCTTACCACAGTGAAAAAT+AGG - tig0020375:5143-5162 None:intergenic 35.0%
! CAAGTTGGATATTTAGAGGA+AGG - tig0020375:5228-5247 None:intergenic 35.0%
! AGGACAAGTTGGATATTTAG+AGG - tig0020375:5232-5251 None:intergenic 35.0%
CCAAACTTAATTGTGTCTCT+AGG - tig0020375:5252-5271 None:intergenic 35.0%
CCTAGAGACACAATTAAGTT+TGG + tig0020375:5249-5268 Msa1380490:CDS 35.0%
GGTTTGATTAATGTTGAGCT+TGG + tig0020375:5291-5310 Msa1380490:CDS 35.0%
GCTCTTAAGGCTAATATCTT+AGG + tig0020375:5390-5409 Msa1380490:CDS 35.0%
! TCTTCCCATTAATTTGAGCT+TGG + tig0020375:5425-5444 Msa1380490:CDS 35.0%
! AGCTTGGTTCTTAACTATTG+TGG + tig0020375:5441-5460 Msa1380490:CDS 35.0%
GGTTCTTAACTATTGTGGAA+AGG + tig0020375:5446-5465 Msa1380490:CDS 35.0%
ATTTAGAGGAAGGTGGATGT+GGG - tig0020375:5218-5237 None:intergenic 40.0%
TATTTAGAGGAAGGTGGATG+TGG - tig0020375:5219-5238 None:intergenic 40.0%
GTTGGATATTTAGAGGAAGG+TGG - tig0020375:5225-5244 None:intergenic 40.0%
!! ATTGTGTCTCTAGGACAAGT+TGG - tig0020375:5243-5262 None:intergenic 40.0%
! TAGCCTTAAGAGCAGTACAA+AGG - tig0020375:5383-5402 None:intergenic 40.0%
TGTGATCTTTGTGAGGTGGT+GGG - tig0020375:5185-5204 None:intergenic 45.0%
TTGTGGCTGTGATCTTTGTG+AGG - tig0020375:5192-5211 None:intergenic 45.0%
! TGGTGTGTGTGCTGATGTAT+TGG + tig0020375:5269-5288 Msa1380490:CDS 45.0%
! GGTGTGTGTGCTGATGTATT+GGG + tig0020375:5270-5289 Msa1380490:CDS 45.0%
GTGCCTTTGTACTGCTCTTA+AGG + tig0020375:5377-5396 Msa1380490:CDS 45.0%
TTGTGGAAAGGGAGTTCCAA+AGG + tig0020375:5458-5477 Msa1380490:CDS 45.0%
TGTGGAAAGGGAGTTCCAAA+GGG + tig0020375:5459-5478 Msa1380490:CDS 45.0%
TAAGCACACACGAAACCCTT+TGG - tig0020375:5477-5496 None:intergenic 45.0%
! GCAAAGGAGCATAGCAACCT+TGG - tig0020375:5112-5131 None:intergenic 50.0%
! TGATCTTTGTGAGGTGGTGG+GGG - tig0020375:5183-5202 None:intergenic 50.0%
GTGATCTTTGTGAGGTGGTG+GGG - tig0020375:5184-5203 None:intergenic 50.0%
CTGTGATCTTTGTGAGGTGG+TGG - tig0020375:5186-5205 None:intergenic 50.0%
TGGCTGTGATCTTTGTGAGG+TGG - tig0020375:5189-5208 None:intergenic 50.0%
AAGGTGGATGTGGGTGTTTG+TGG - tig0020375:5209-5228 None:intergenic 50.0%
AGAGAGCAGCATGGTGTCTT+TGG - tig0020375:5324-5343 None:intergenic 50.0%
CCATCAATGAGAGAGCAGCA+TGG - tig0020375:5333-5352 None:intergenic 50.0%
CCATGCTGCTCTCTCATTGA+TGG + tig0020375:5330-5349 Msa1380490:CDS 50.0%
GAGCAGCATGGTGTCTTTGG+TGG - tig0020375:5321-5340 None:intergenic 55.0%
!! TTGTGAGGTGGTGGGGGACA+TGG - tig0020375:5177-5196 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0020375 gene 5102 5497 5102 ID=Msa1380490;Name=Msa1380490
tig0020375 mRNA 5102 5497 5102 ID=Msa1380490-mRNA-1;Parent=Msa1380490;Name=Msa1380490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|131
tig0020375 exon 5102 5497 5102 ID=Msa1380490-mRNA-1:exon:7150;Parent=Msa1380490-mRNA-1
tig0020375 CDS 5102 5497 5102 ID=Msa1380490-mRNA-1:cds;Parent=Msa1380490-mRNA-1
Gene Sequence

>Msa1380490

ATGGCTTCCAAGGTTGCTATGCTCCTTTGCCTCAACATCCTATTTTTCACTGTGGTAAGCTCTACATATGTTCCATGTCCCCCACCACCTCACAAAGATCACAGCCACAAACACCCACATCCACCTTCCTCTAAATATCCAACTTGTCCTAGAGACACAATTAAGTTTGGTGTGTGTGCTGATGTATTGGGTTTGATTAATGTTGAGCTTGGTAAGCCACCAAAGACACCATGCTGCTCTCTCATTGATGGTCTTGCTAATCTTGAAGCTGCTGTGTGCCTTTGTACTGCTCTTAAGGCTAATATCTTAGGCATTAACCTTAATCTTCCCATTAATTTGAGCTTGGTTCTTAACTATTGTGGAAAGGGAGTTCCAAAGGGTTTCGTGTGTGCTTAA

Protein sequence

>Msa1380490

MASKVAMLLCLNILFFTVVSSTYVPCPPPPHKDHSHKHPHPPSSKYPTCPRDTIKFGVCADVLGLINVELGKPPKTPCCSLIDGLANLEAAVCLCTALKANILGINLNLPINLSLVLNYCGKGVPKGFVCA*