AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0859560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859560 MtrunA17_Chr5g0438511 51.852 108 44 2 1 101 1 107 3.46e-20 80.1
Msa0859560 MtrunA17_Chr5g0438501 76.000 50 12 0 1 50 1 50 3.90e-19 77.0
Msa0859560 MtrunA17_Chr5g0438411 48.507 134 36 3 1 102 1 133 6.57e-19 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATATAGCTCACCTGTTT+TGG 0.281946 5_4:-57908437 None:intergenic
TTGTTCTTCTTATTTCATCT+AGG 0.334276 5_4:+57908377 Msa0859560:CDS
TAAGAAGAACAATAGCAAAT+AGG 0.365064 5_4:-57908366 None:intergenic
TGTTCTTCTTATTTCATCTA+GGG 0.409997 5_4:+57908378 Msa0859560:CDS
ATACAAATCGATATTTCAAA+AGG 0.428471 5_4:-57908287 None:intergenic
GGCAATGGTTGTTGCAGTCC+TGG 0.429599 5_4:+57910655 Msa0859560:CDS
AGGATCATAGACTCCATCTT+TGG 0.433604 5_4:-57910599 None:intergenic
GAACAATAGCAAATAGGCCT+TGG 0.437760 5_4:-57908360 None:intergenic
ATTCATGCAATATATTACTA+AGG 0.453267 5_4:-57910802 None:intergenic
ACTTCCTCTAATACCAAAAC+AGG 0.456252 5_4:+57908424 Msa0859560:CDS
GAAATAAATGATGCCAAAGA+TGG 0.474863 5_4:+57910586 Msa0859560:CDS
GGAGTCTATGATCCTTCCCC+TGG 0.483993 5_4:+57910607 Msa0859560:CDS
GTTCCTCCATGTCTACCTCC+AGG 0.490643 5_4:-57910625 None:intergenic
TTCATCTAGGGTTTCAGCGA+GGG 0.490829 5_4:+57908390 Msa0859560:CDS
TGATGATATCTTAATTGTGA+GGG 0.494408 5_4:-57910750 None:intergenic
GGAGGAACTTATTGCGGCAA+TGG 0.511558 5_4:+57910640 Msa0859560:CDS
CCTTCCCCTGGAGGTAGACA+TGG 0.519197 5_4:+57910619 Msa0859560:CDS
TTCCTCCATGTCTACCTCCA+GGG 0.523377 5_4:-57910624 None:intergenic
TACAAATCGATATTTCAAAA+GGG 0.542278 5_4:-57908286 None:intergenic
TTGATGATATCTTAATTGTG+AGG 0.548101 5_4:-57910751 None:intergenic
CAATTCGAATATAAGCTGCC+AGG 0.549080 5_4:-57910673 None:intergenic
GTCTATGATCCTTCCCCTGG+AGG 0.551026 5_4:+57910610 Msa0859560:CDS
AATTATACTCTTAAGTACAA+TGG 0.554593 5_4:+57908315 Msa0859560:exon
GTGTTGAATTTGATCAAAAG+AGG 0.562484 5_4:+57910870 Msa0859560:three_prime_UTR
CCATGTCTACCTCCAGGGGA+AGG 0.578483 5_4:-57910619 None:intergenic
TTAATTGTGAGGGTTATCCT+CGG 0.581656 5_4:-57910740 None:intergenic
TAGCAAATAGGCCTTGGATG+AGG 0.583802 5_4:-57908354 None:intergenic
TCCTCCATGTCTACCTCCAG+GGG 0.608221 5_4:-57910623 None:intergenic
AGACATGGAGGAACTTATTG+CGG 0.635738 5_4:+57910634 Msa0859560:CDS
TTTCATCTAGGGTTTCAGCG+AGG 0.666165 5_4:+57908389 Msa0859560:CDS
AGCTGTTGCTGTGCAAACCG+AGG 0.669654 5_4:+57910723 Msa0859560:CDS
TCCCCTGGAGGTAGACATGG+AGG 0.673287 5_4:+57910622 Msa0859560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAATAAATAAATAA+TGG + chr5_4:57910844-57910863 Msa0859560:three_prime_UTR 0.0%
!!! AACAAAATTAATTTTCTTTT+TGG + chr5_4:57908682-57908701 Msa0859560:intron 10.0%
!!! ATATTTTTTTATATTGTACA+AGG + chr5_4:57909111-57909130 Msa0859560:intron 10.0%
!!! TTTAATTCCTTTTTTTTTTT+TGG + chr5_4:57909794-57909813 Msa0859560:intron 10.0%
!! TGAATAAAAATTAAAGAATA+AGG - chr5_4:57909838-57909857 None:intergenic 10.0%
!! ATACTTATAAAATTCAAAAG+AGG - chr5_4:57908514-57908533 None:intergenic 15.0%
!!! GAAAATTTTAAGTTTTGTAT+TGG + chr5_4:57908612-57908631 Msa0859560:intron 15.0%
!! TTTAACAGATATGATTTATT+TGG - chr5_4:57909007-57909026 None:intergenic 15.0%
!! ACATTTGAACTATATTATTA+AGG + chr5_4:57909137-57909156 Msa0859560:intron 15.0%
!! ATATAATCATAATATATGCA+AGG + chr5_4:57909211-57909230 Msa0859560:intron 15.0%
!! CAAAACAAGAAAAAAAATTA+AGG + chr5_4:57909264-57909283 Msa0859560:intron 15.0%
!!! TCTCATTTTTTTTTTTTGAA+AGG - chr5_4:57909442-57909461 None:intergenic 15.0%
!! AATTATACTCTTAAGTACAA+TGG + chr5_4:57908315-57908334 Msa0859560:exon 20.0%
!!! TGCAATTTAGCTTTTTTTTA+GGG - chr5_4:57908894-57908913 None:intergenic 20.0%
!!! ATGCAATTTAGCTTTTTTTT+AGG - chr5_4:57908895-57908914 None:intergenic 20.0%
!! GAAAACAACAAATAAGTTAA+GGG - chr5_4:57908925-57908944 None:intergenic 20.0%
!! TGAAAACAACAAATAAGTTA+AGG - chr5_4:57908926-57908945 None:intergenic 20.0%
!!! CTTATTTGTTGTTTTCATTT+TGG + chr5_4:57908929-57908948 Msa0859560:intron 20.0%
!! AAATTTAATTAGGTCTTTGA+TGG - chr5_4:57909073-57909092 None:intergenic 20.0%
!!! AATTCAATGTCATTTTTAAG+TGG - chr5_4:57909380-57909399 None:intergenic 20.0%
!!! AATTCAATGTCATTTTTAAG+TGG - chr5_4:57909380-57909399 None:intergenic 20.0%
!! ACTACATCCAAAAAAAAAAA+AGG - chr5_4:57909804-57909823 None:intergenic 20.0%
!! TATATATATAACCATCCATT+AGG + chr5_4:57910219-57910238 Msa0859560:intron 20.0%
!! CTATAAAATAATTGTTCTCA+AGG - chr5_4:57910476-57910495 None:intergenic 20.0%
!!! ATATATAATTTGTTTGCAGA+TGG + chr5_4:57910547-57910566 Msa0859560:intron 20.0%
!! ATTCATGCAATATATTACTA+AGG - chr5_4:57910805-57910824 None:intergenic 20.0%
! TAAGAAGAACAATAGCAAAT+AGG - chr5_4:57908369-57908388 None:intergenic 25.0%
!! TTGTTCTTCTTATTTCATCT+AGG + chr5_4:57908377-57908396 Msa0859560:CDS 25.0%
!! TGTTCTTCTTATTTCATCTA+GGG + chr5_4:57908378-57908397 Msa0859560:CDS 25.0%
! ATAGGGAAAAAAAAGTTTGT+TGG - chr5_4:57908659-57908678 None:intergenic 25.0%
! CTTAAGTGTTAAGTTCTTAT+GGG - chr5_4:57908716-57908735 None:intergenic 25.0%
! AAAAAGCTAAATTGCATACT+TGG + chr5_4:57908898-57908917 Msa0859560:intron 25.0%
!!! TTTCTTGTTTTGTTGTTTTG+TGG - chr5_4:57909256-57909275 None:intergenic 25.0%
! TTAAGGATATATGAGACTTA+AGG + chr5_4:57909281-57909300 Msa0859560:intron 25.0%
!! TATTCTAAAAACTATTTGCG+TGG + chr5_4:57909344-57909363 Msa0859560:intron 25.0%
!! TATTCTAAAAACTATTTGCG+TGG + chr5_4:57909344-57909363 Msa0859560:intron 25.0%
! AGAAGATTGTTTGTAAGAAA+GGG - chr5_4:57909687-57909706 None:intergenic 25.0%
! CTCCAAAAACCTATATATTT+GGG + chr5_4:57909870-57909889 Msa0859560:intron 25.0%
!! AACCCAAATATATAGGTTTT+TGG - chr5_4:57909875-57909894 None:intergenic 25.0%
! ACTCTAGATAAATTCTTACT+TGG - chr5_4:57909903-57909922 None:intergenic 25.0%
! ATATATATACACACACTCAT+CGG - chr5_4:57910124-57910143 None:intergenic 25.0%
! ATATTGTAGCTATGAGATTT+GGG + chr5_4:57910377-57910396 Msa0859560:intron 25.0%
! ATCTCTGTTAATATTCCAAA+CGG + chr5_4:57910401-57910420 Msa0859560:intron 25.0%
! TGATGATATCTTAATTGTGA+GGG - chr5_4:57910753-57910772 None:intergenic 25.0%
!! TTGATGATATCTTAATTGTG+AGG - chr5_4:57910754-57910773 None:intergenic 25.0%
! ATTATATAGCTCACCTGTTT+TGG - chr5_4:57908440-57908459 None:intergenic 30.0%
GCTTAAGTGTTAAGTTCTTA+TGG - chr5_4:57908717-57908736 None:intergenic 30.0%
CTAACAAAGTACAAGTTAAG+GGG - chr5_4:57908984-57909003 None:intergenic 30.0%
GCTAACAAAGTACAAGTTAA+GGG - chr5_4:57908985-57909004 None:intergenic 30.0%
AAATGTTAACCGAATGTCTA+AGG + chr5_4:57909026-57909045 Msa0859560:intron 30.0%
ATGTATTCACCTTAGACATT+CGG - chr5_4:57909038-57909057 None:intergenic 30.0%
AGGTGAATACATATTGTAAG+TGG + chr5_4:57909046-57909065 Msa0859560:intron 30.0%
ATCATAATATATGCAAGGTC+CGG + chr5_4:57909216-57909235 Msa0859560:intron 30.0%
GAGAAGATTGTTTGTAAGAA+AGG - chr5_4:57909688-57909707 None:intergenic 30.0%
GATTCTAATGAAAGCAAAGA+GGG - chr5_4:57909740-57909759 None:intergenic 30.0%
TGATTCTAATGAAAGCAAAG+AGG - chr5_4:57909741-57909760 None:intergenic 30.0%
TCTTTGCTTTCATTAGAATC+AGG + chr5_4:57909740-57909759 Msa0859560:intron 30.0%
GCTCCAAAAACCTATATATT+TGG + chr5_4:57909869-57909888 Msa0859560:intron 30.0%
AAGATGTAAGTCATTTGTGT+CGG + chr5_4:57909923-57909942 Msa0859560:intron 30.0%
TAAACTATGAACATGCATGA+TGG - chr5_4:57910329-57910348 None:intergenic 30.0%
CATATTGTAGCTATGAGATT+TGG + chr5_4:57910376-57910395 Msa0859560:intron 30.0%
ATAGTTTCTTGACAATTGTG+AGG + chr5_4:57910492-57910511 Msa0859560:intron 30.0%
GAAATAAATGATGCCAAAGA+TGG + chr5_4:57910586-57910605 Msa0859560:CDS 30.0%
AAAAAAGCAATCCTCATCCA+AGG + chr5_4:57908343-57908362 Msa0859560:CDS 35.0%
ACTTCCTCTAATACCAAAAC+AGG + chr5_4:57908424-57908443 Msa0859560:CDS 35.0%
AGTATGCTTGTAAGTGATGT+TGG + chr5_4:57908529-57908548 Msa0859560:intron 35.0%
ATATCATTTGCATGAGCCTT+AGG - chr5_4:57908578-57908597 None:intergenic 35.0%
AAGGCTCATGCAAATGATAT+TGG + chr5_4:57908578-57908597 Msa0859560:intron 35.0%
!! AGTTTGTTGGACATTTACGT+GGG - chr5_4:57908646-57908665 None:intergenic 35.0%
!! AAGTTTGTTGGACATTTACG+TGG - chr5_4:57908647-57908666 None:intergenic 35.0%
! GACTTGGAGTAGTTTTTGAA+TGG + chr5_4:57908743-57908762 Msa0859560:intron 35.0%
! TTTCTGAGAAACTTCTCAGA+AGG - chr5_4:57908772-57908791 None:intergenic 35.0%
!! AAAGACTCGTCTTGATTTGT+AGG + chr5_4:57908820-57908839 Msa0859560:intron 35.0%
!! AAGACTCGTCTTGATTTGTA+GGG + chr5_4:57908821-57908840 Msa0859560:intron 35.0%
GGCTAACAAAGTACAAGTTA+AGG - chr5_4:57908986-57909005 None:intergenic 35.0%
TCATAATATATGCAAGGTCC+GGG + chr5_4:57909217-57909236 Msa0859560:intron 35.0%
!!! TTGTTTTGTTGTTTTGTGGC+CGG - chr5_4:57909252-57909271 None:intergenic 35.0%
!!! AGACGTACTCTTTTTTTGAC+AGG + chr5_4:57909311-57909330 Msa0859560:intron 35.0%
!!! AGACGTACTCTTTTTTTGAC+AGG + chr5_4:57909311-57909330 Msa0859560:intron 35.0%
ATCTTCGTCAATAGACGATT+AGG + chr5_4:57910047-57910066 Msa0859560:intron 35.0%
TCTTCGTCAATAGACGATTA+GGG + chr5_4:57910048-57910067 Msa0859560:intron 35.0%
AACGAGATCACTATGAGAAA+TGG - chr5_4:57910084-57910103 None:intergenic 35.0%
GTGTATTCTAACCTAATGGA+TGG - chr5_4:57910233-57910252 None:intergenic 35.0%
ATGAGTGTTTGTGATGTGTT+TGG - chr5_4:57910261-57910280 None:intergenic 35.0%
! AGAGGTAGATTAGCTCTTAA+TGG + chr5_4:57910286-57910305 Msa0859560:intron 35.0%
GAAAAGAAGATCAAACCGTT+TGG - chr5_4:57910419-57910438 None:intergenic 35.0%
AAAAAATGCTGCTCATATGC+TGG + chr5_4:57910697-57910716 Msa0859560:CDS 35.0%
TTAATTGTGAGGGTTATCCT+CGG - chr5_4:57910743-57910762 None:intergenic 35.0%
GAACAATAGCAAATAGGCCT+TGG - chr5_4:57908363-57908382 None:intergenic 40.0%
!!! CTCACCTGTTTTGGTATTAG+AGG - chr5_4:57908431-57908450 None:intergenic 40.0%
TTGCATTTCGTAGCAACCTA+AGG + chr5_4:57908559-57908578 Msa0859560:intron 40.0%
TTTGTTGGACATTTACGTGG+GGG - chr5_4:57908644-57908663 None:intergenic 40.0%
! GTTTGTTGGACATTTACGTG+GGG - chr5_4:57908645-57908664 None:intergenic 40.0%
ACACTTAAGCATGCTTGACT+TGG + chr5_4:57908727-57908746 Msa0859560:intron 40.0%
CATAATATATGCAAGGTCCG+GGG + chr5_4:57909218-57909237 Msa0859560:intron 40.0%
!!! TGTTTTGTTGTTTTGTGGCC+GGG - chr5_4:57909251-57909270 None:intergenic 40.0%
GGTCGCAAACCCAAATATAT+AGG - chr5_4:57909882-57909901 None:intergenic 40.0%
TATATACACACACTCATCGG+TGG - chr5_4:57910121-57910140 None:intergenic 40.0%
TGTTTGTGATGTGTTTGGTC+GGG - chr5_4:57910256-57910275 None:intergenic 40.0%
GTGTTTGTGATGTGTTTGGT+CGG - chr5_4:57910257-57910276 None:intergenic 40.0%
CACAAACACTCATCAGTAAG+AGG + chr5_4:57910268-57910287 Msa0859560:intron 40.0%
AGGATCATAGACTCCATCTT+TGG - chr5_4:57910602-57910621 None:intergenic 40.0%
AGACATGGAGGAACTTATTG+CGG + chr5_4:57910634-57910653 Msa0859560:CDS 40.0%
CAATTCGAATATAAGCTGCC+AGG - chr5_4:57910676-57910695 None:intergenic 40.0%
TAGCAAATAGGCCTTGGATG+AGG - chr5_4:57908357-57908376 None:intergenic 45.0%
! TTTCATCTAGGGTTTCAGCG+AGG + chr5_4:57908389-57908408 Msa0859560:CDS 45.0%
! TTCATCTAGGGTTTCAGCGA+GGG + chr5_4:57908390-57908409 Msa0859560:CDS 45.0%
!!! GTTTTGTTGTTTTGTGGCCG+GGG - chr5_4:57909250-57909269 None:intergenic 45.0%
CGGGGTGTATTCTAACCTAA+TGG - chr5_4:57910237-57910256 None:intergenic 45.0%
GTTTGTGATGTGTTTGGTCG+GGG - chr5_4:57910255-57910274 None:intergenic 45.0%
AGTCGAGCTGTGAAAGAAGT+TGG - chr5_4:57910441-57910460 None:intergenic 45.0%
!!! AAATTAATTTTGTTTATATA+GGG - chr5_4:57908676-57908695 None:intergenic 5.0%
!!! AAAATTAATTTTGTTTATAT+AGG - chr5_4:57908677-57908696 None:intergenic 5.0%
!! TAGTATTAAAAAATTTAATT+AGG - chr5_4:57909083-57909102 None:intergenic 5.0%
!!! TTAAATATATTTTTTTTGAA+AGG - chr5_4:57909602-57909621 None:intergenic 5.0%
!!! CTAAAATTAAATATATTTTT+CGG - chr5_4:57909637-57909656 None:intergenic 5.0%
!!! ATATATTTAATTTTAGTTAT+AGG + chr5_4:57909641-57909660 Msa0859560:intron 5.0%
! TCTCAGAAAGCGTGCGAATG+AGG + chr5_4:57908783-57908802 Msa0859560:intron 50.0%
TTCCTCCATGTCTACCTCCA+GGG - chr5_4:57910627-57910646 None:intergenic 50.0%
GGAGGAACTTATTGCGGCAA+TGG + chr5_4:57910640-57910659 Msa0859560:CDS 50.0%
GGAGTCTATGATCCTTCCCC+TGG + chr5_4:57910607-57910626 Msa0859560:CDS 55.0%
GTCTATGATCCTTCCCCTGG+AGG + chr5_4:57910610-57910629 Msa0859560:CDS 55.0%
TCCTCCATGTCTACCTCCAG+GGG - chr5_4:57910626-57910645 None:intergenic 55.0%
GTTCCTCCATGTCTACCTCC+AGG - chr5_4:57910628-57910647 None:intergenic 55.0%
GGCAATGGTTGTTGCAGTCC+TGG + chr5_4:57910655-57910674 Msa0859560:CDS 55.0%
AGCTGTTGCTGTGCAAACCG+AGG + chr5_4:57910723-57910742 Msa0859560:CDS 55.0%
AAGGTCCGGGGTTCAAACCC+CGG + chr5_4:57909230-57909249 Msa0859560:intron 60.0%
CCATGTCTACCTCCAGGGGA+AGG - chr5_4:57910622-57910641 None:intergenic 60.0%
CCTTCCCCTGGAGGTAGACA+TGG + chr5_4:57910619-57910638 Msa0859560:CDS 60.0%
TCCCCTGGAGGTAGACATGG+AGG + chr5_4:57910622-57910641 Msa0859560:CDS 60.0%
TGTGGCCGGGGTTTGAACCC+CGG - chr5_4:57909238-57909257 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 57908288 57910889 57908288 ID=Msa0859560;Name=Msa0859560
chr5_4 mRNA 57908288 57910889 57908288 ID=Msa0859560-mRNA-1;Parent=Msa0859560;Name=Msa0859560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=46|1|1|1|1|1|2|127|102
chr5_4 exon 57908288 57908445 57908288 ID=Msa0859560-mRNA-1:exon:17651;Parent=Msa0859560-mRNA-1
chr5_4 exon 57910566 57910889 57910566 ID=Msa0859560-mRNA-1:exon:17652;Parent=Msa0859560-mRNA-1
chr5_4 five_prime_UTR 57908288 57908333 57908288 ID=Msa0859560-mRNA-1:five_prime_utr;Parent=Msa0859560-mRNA-1
chr5_4 CDS 57908334 57908445 57908334 ID=Msa0859560-mRNA-1:cds;Parent=Msa0859560-mRNA-1
chr5_4 CDS 57910566 57910762 57910566 ID=Msa0859560-mRNA-1:cds;Parent=Msa0859560-mRNA-1
chr5_4 three_prime_UTR 57910763 57910889 57910763 ID=Msa0859560-mRNA-1:three_prime_utr;Parent=Msa0859560-mRNA-1
Gene Sequence

>Msa0859560

ATGGATTCAAAAAAAGCAATCCTCATCCAAGGCCTATTTGCTATTGTTCTTCTTATTTCATCTAGGGTTTCAGCGAGGGACTTAACTGAGACTTCCTCTAATACCAAAACAGATGGTGTTGAACAGACAAATGAAATAAATGATGCCAAAGATGGAGTCTATGATCCTTCCCCTGGAGGTAGACATGGAGGAACTTATTGCGGCAATGGTTGTTGCAGTCCTGGCAGCTTATATTCGAATTGCAAAAAATGCTGCTCATATGCTGGCGAAGCTGTTGCTGTGCAAACCGAGGATAACCCTCACAATTAA

Protein sequence

>Msa0859560

MDSKKAILIQGLFAIVLLISSRVSARDLTETSSNTKTDGVEQTNEINDAKDGVYDPSPGGRHGGTYCGNGCCSPGSLYSNCKKCCSYAGEAVAVQTEDNPHN*