AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0553150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553150 MtrunA17_Chr4g0038821 97.059 68 2 0 1 68 1 68 1.37e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553150 AT3G22210.1 62.500 64 23 1 5 68 6 68 1.55e-20 77.0

Find 19 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGAACATGCAACTATTT+TGG 0.213384 4_1:-50058049 None:intergenic
GCAATTCGCTCTTCCAGTTT+TGG 0.231911 4_1:+50057488 Msa0553150:CDS
CGATGGAGAAAAGTCATTTA+AGG 0.322010 4_1:-50058083 None:intergenic
GTCTTCTAGCTCGCCTCTCT+CGG 0.346161 4_1:-50057718 None:intergenic
GACAGAAATCACTTCAAGAT+TGG 0.349085 4_1:+50057648 Msa0553150:intron
GCTGCAACAATGCCCAAAAC+TGG 0.365881 4_1:-50057501 None:intergenic
AGCTCGCCTCTCTCGGGAGC+TGG 0.377870 4_1:-50057711 None:intergenic
GAGAGATATATCATCAGAAA+TGG 0.429163 4_1:+50057458 Msa0553150:exon
AGTAGCAAATTGTTTGTACT+AGG 0.459932 4_1:+50057853 Msa0553150:three_prime_UTR
ACAGAAATCACTTCAAGATT+GGG 0.482353 4_1:+50057649 Msa0553150:intron
ATTGAAACTCACTGCATAGA+AGG 0.485260 4_1:-50057535 None:intergenic
TCTTCTAGCTCGCCTCTCTC+GGG 0.500761 4_1:-50057717 None:intergenic
ACCAACAAGAACTATTGAAA+AGG 0.500770 4_1:-50057884 None:intergenic
CTCTCTCGGGAGCTGGACGT+TGG 0.528180 4_1:-50057704 None:intergenic
GATGATTCTGAATCAGAGAA+TGG 0.538128 4_1:+50057671 Msa0553150:CDS
CAACGTCCAGCTCCCGAGAG+AGG 0.600475 4_1:+50057705 Msa0553150:CDS
GAGTTTCAATGAGATTAGAG+AGG 0.641117 4_1:+50057548 Msa0553150:CDS
GATTCTGAATCAGAGAATGG+AGG 0.645594 4_1:+50057674 Msa0553150:CDS
GTGAGACTAGTCACTGACCC+AGG 0.670217 4_1:-50058005 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACATTTTTCTAATAGAAAA+TGG - chr4_1:50057933-50057952 None:intergenic 15.0%
!!! ATTTCTTTATTTTTGATGCT+TGG + chr4_1:50057813-50057832 Msa0553150:three_prime_UTR 20.0%
!!! TTGCTGTTGAAGTTATTTTT+TGG - chr4_1:50057759-50057778 None:intergenic 25.0%
!! AAATGAACATGCAACTATTT+TGG - chr4_1:50058052-50058071 None:intergenic 25.0%
GAGAGATATATCATCAGAAA+TGG + chr4_1:50057458-50057477 Msa0553150:exon 30.0%
ACAGAAATCACTTCAAGATT+GGG + chr4_1:50057649-50057668 Msa0553150:intron 30.0%
AGTAGCAAATTGTTTGTACT+AGG + chr4_1:50057853-50057872 Msa0553150:three_prime_UTR 30.0%
! ACCTTTTCAATAGTTCTTGT+TGG + chr4_1:50057883-50057902 Msa0553150:three_prime_UTR 30.0%
ACCAACAAGAACTATTGAAA+AGG - chr4_1:50057887-50057906 None:intergenic 30.0%
!!! ACATTTTTTGTGTTTTCTCC+TGG + chr4_1:50057987-50058006 Msa0553150:three_prime_UTR 30.0%
!!! CATTTTTTGTGTTTTCTCCT+GGG + chr4_1:50057988-50058007 Msa0553150:three_prime_UTR 30.0%
ATTGAAACTCACTGCATAGA+AGG - chr4_1:50057538-50057557 None:intergenic 35.0%
GAGTTTCAATGAGATTAGAG+AGG + chr4_1:50057548-50057567 Msa0553150:CDS 35.0%
GACAGAAATCACTTCAAGAT+TGG + chr4_1:50057648-50057667 Msa0553150:intron 35.0%
GATGATTCTGAATCAGAGAA+TGG + chr4_1:50057671-50057690 Msa0553150:CDS 35.0%
! CAATTCGCTCTTCCAGTTTT+GGG + chr4_1:50057489-50057508 Msa0553150:CDS 40.0%
GATTCTGAATCAGAGAATGG+AGG + chr4_1:50057674-50057693 Msa0553150:CDS 40.0%
!!! CATGCAACTATTTTGGTCTC+TGG - chr4_1:50058045-50058064 None:intergenic 40.0%
! GCAATTCGCTCTTCCAGTTT+TGG + chr4_1:50057488-50057507 Msa0553150:CDS 45.0%
GCTGCAACAATGCCCAAAAC+TGG - chr4_1:50057504-50057523 None:intergenic 50.0%
!! TCTTCTAGCTCGCCTCTCTC+GGG - chr4_1:50057720-50057739 None:intergenic 55.0%
!! GTCTTCTAGCTCGCCTCTCT+CGG - chr4_1:50057721-50057740 None:intergenic 55.0%
! GTGAGACTAGTCACTGACCC+AGG - chr4_1:50058008-50058027 None:intergenic 55.0%
CTCTCTCGGGAGCTGGACGT+TGG - chr4_1:50057707-50057726 None:intergenic 65.0%
CAACGTCCAGCTCCCGAGAG+AGG + chr4_1:50057705-50057724 Msa0553150:CDS 65.0%
AGCTCGCCTCTCTCGGGAGC+TGG - chr4_1:50057714-50057733 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 50057428 50058099 50057428 ID=Msa0553150;Name=Msa0553150
chr4_1 mRNA 50057428 50058099 50057428 ID=Msa0553150-mRNA-1;Parent=Msa0553150;Name=Msa0553150-mRNA-1;_AED=0.01;_eAED=0.01;_QI=49|1|1|1|1|1|2|333|68
chr4_1 exon 50057428 50057569 50057428 ID=Msa0553150-mRNA-1:exon:14110;Parent=Msa0553150-mRNA-1
chr4_1 exon 50057653 50058099 50057653 ID=Msa0553150-mRNA-1:exon:14111;Parent=Msa0553150-mRNA-1
chr4_1 five_prime_UTR 50057428 50057476 50057428 ID=Msa0553150-mRNA-1:five_prime_utr;Parent=Msa0553150-mRNA-1
chr4_1 CDS 50057477 50057569 50057477 ID=Msa0553150-mRNA-1:cds;Parent=Msa0553150-mRNA-1
chr4_1 CDS 50057653 50057766 50057653 ID=Msa0553150-mRNA-1:cds;Parent=Msa0553150-mRNA-1
chr4_1 three_prime_UTR 50057767 50058099 50057767 ID=Msa0553150-mRNA-1:three_prime_utr;Parent=Msa0553150-mRNA-1
Gene Sequence

>Msa0553150

ATGGATGCAATGCAATTCGCTCTTCCAGTTTTGGGCATTGTTGCAGCTGCAGCTGTGACCTTCTATGCAGTGAGTTTCAATGAGATTAGAGAGAAATCACTTCAAGATTGGGATGATTCTGAATCAGAGAATGGAGGTTTTAGACCAACGTCCAGCTCCCGAGAGAGGCGAGCTAGAAGACAAGCTAGTAAAAATACCAAAAAATAA

Protein sequence

>Msa0553150

MDAMQFALPVLGIVAAAAVTFYAVSFNEIREKSLQDWDDSESENGGFRPTSSSRERRARRQASKNTKK*