AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0902220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0902220 MtrunA17_Chr6g0484891 97.906 191 4 0 1 191 1 191 1.73e-137 381
Msa0902220 MtrunA17_Chr6g0458031 97.382 191 5 0 1 191 1 191 3.80e-137 380
Msa0902220 MtrunA17_Chr8g0379651 63.636 44 8 1 145 188 51 86 6.20e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0902220 AT1G48830.1 79.058 191 40 0 1 191 1 191 1.54e-114 324
Msa0902220 AT1G48830.2 79.058 191 40 0 1 191 1 191 1.54e-114 324
Msa0902220 AT3G02560.1 79.581 191 39 0 1 191 1 191 5.73e-113 320
Msa0902220 AT3G02560.3 79.581 191 39 0 1 191 1 191 5.73e-113 320
Msa0902220 AT3G02560.2 79.581 191 39 0 1 191 1 191 5.73e-113 320
Msa0902220 AT5G16130.1 80.319 188 37 0 1 188 1 188 2.00e-112 318

Find 73 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGCCAAGTTGATGTTTC+TGG 0.145762 6_1:+100058693 Msa0902220:intron
ATATGATGAATGTTTATTTA+TGG 0.222500 6_1:+100059872 Msa0902220:three_prime_UTR
GTTGAAGCTCTCTATGATTC+TGG 0.238608 6_1:+100059904 Msa0902220:three_prime_UTR
TATGATACATTATTTATTAT+AGG 0.254565 6_1:+100059948 Msa0902220:three_prime_UTR
TCCTCAATCTGTAGGGGATA+TGG 0.261869 6_1:-100058737 None:intergenic
AGAATCTACGCCTCAGTAAC+AGG 0.280462 6_1:-100059813 None:intergenic
TTTGTTAACCTATATAGTTT+TGG 0.282836 6_1:-100056262 None:intergenic
TGAGCAGCGGAGCCTTTCTT+TGG 0.288203 6_1:-100059365 None:intergenic
ATAGTTTACCTTCATGATCT+TGG 0.292174 6_1:-100059495 None:intergenic
TTATTATGAAAAGTCTTTCT+TGG 0.331298 6_1:-100060031 None:intergenic
CAACACATATATTTACAAAA+TGG 0.341102 6_1:-100056225 None:intergenic
TAAACCAAGAGTGTTTCATT+AGG 0.349218 6_1:+100056291 Msa0902220:five_prime_UTR
AAATTCACGTCAAGCTCGTT+AGG 0.363074 6_1:+100058772 Msa0902220:CDS
CGTACTAGATACAGAATTGA+TGG 0.367485 6_1:+100059470 Msa0902220:CDS
TATAATAAATAATGTATCAT+AGG 0.369192 6_1:-100059946 None:intergenic
TATTATGAAAAGTCTTTCTT+GGG 0.377421 6_1:-100060030 None:intergenic
AAACCAAGAGTGTTTCATTA+GGG 0.381952 6_1:+100056292 Msa0902220:five_prime_UTR
GTTGCAGGCTTTGTTTGATC+TGG 0.412438 6_1:+100058532 Msa0902220:intron
CGCTGAGCCTACTGAATTTG+AGG 0.426295 6_1:+100058399 Msa0902220:CDS
ACACAAGCCCAAAACTATAT+AGG 0.431675 6_1:+100056254 Msa0902220:five_prime_UTR
GCAGCGGAGCCTTTCTTTGG+TGG 0.435938 6_1:-100059362 None:intergenic
TTGTGAATATGTTTACTTCA+AGG 0.441187 6_1:+100058353 Msa0902220:exon
ATCTGCATCTTCTTCGGCTA+AGG 0.454250 6_1:+100056349 Msa0902220:five_prime_UTR
GCAGTTTACAGGAAACTCTC+AGG 0.456482 6_1:+100059768 Msa0902220:CDS
AACTATATAGGTTAACAAAC+GGG 0.460024 6_1:+100056266 Msa0902220:five_prime_UTR
AGCCGCATCTGCATCTTCTT+CGG 0.466019 6_1:+100056343 Msa0902220:five_prime_UTR
ATTTGAGGAGTCAGTTGGAC+AGG 0.472058 6_1:+100058414 Msa0902220:CDS
TGTTACCTGCTGAGATTGTT+GGG 0.479284 6_1:+100059444 Msa0902220:CDS
AAACTATATAGGTTAACAAA+CGG 0.480311 6_1:+100056265 Msa0902220:five_prime_UTR
TGGTGGCCTGACGATTCTCC+TGG 0.483590 6_1:-100059345 None:intergenic
GTGTTACCTGCTGAGATTGT+TGG 0.484291 6_1:+100059443 Msa0902220:CDS
AAACCCTAATGAAACACTCT+TGG 0.492918 6_1:-100056295 None:intergenic
TCCCCTACAGATTGAGGAAG+GGG 0.499187 6_1:+100058742 Msa0902220:CDS
CGTGGACAGCAGTGAGTGTA+CGG 0.505350 6_1:-100059400 None:intergenic
ATCGTCAGGCCACCAAAGAA+AGG 0.508890 6_1:+100059353 Msa0902220:CDS
TTCTGGAAACCGCAAGGCTG+TGG 0.509249 6_1:+100058710 Msa0902220:CDS
TCCATATCCCCTACAGATTG+AGG 0.509912 6_1:+100058736 Msa0902220:CDS
TTGCCACCAGGAGAATCGTC+AGG 0.510117 6_1:+100059339 Msa0902220:CDS
GAAGAAAATCAGCAAGGACA+AGG 0.510615 6_1:+100058375 Msa0902220:CDS
GAACCCCTTCCTCAATCTGT+AGG 0.515858 6_1:-100058745 None:intergenic
AACCCCTTCCTCAATCTGTA+GGG 0.520673 6_1:-100058744 None:intergenic
ACTGAATTTGAGGAGTCAGT+TGG 0.548538 6_1:+100058409 Msa0902220:CDS
GCGCGCGAGAAGTGTGAAGG+TGG 0.549394 6_1:+100056166 None:intergenic
AATTCACGTCAAGCTCGTTA+GGG 0.550766 6_1:+100058773 Msa0902220:CDS
GCTGCGTGGACGCTGAGCAG+CGG 0.558388 6_1:-100059378 None:intergenic
CGTTTCCCAACAATCTCAGC+AGG 0.558735 6_1:-100059449 None:intergenic
GTTTGAGTACCCTGTTACTG+AGG 0.572498 6_1:+100059803 Msa0902220:CDS
TATCCCCTACAGATTGAGGA+AGG 0.573232 6_1:+100058740 Msa0902220:CDS
GGTTTCCAGAAACATCAACT+TGG 0.575562 6_1:-100058698 None:intergenic
TAAATGATATATTAAAATGA+CGG 0.578845 6_1:+100060084 Msa0902220:three_prime_UTR
ATGTAATCCTCATTGCCACC+AGG 0.584954 6_1:+100059327 Msa0902220:CDS
CGATTCTCCTGGTGGCAATG+AGG 0.590532 6_1:-100059334 None:intergenic
CGCGCGAGAAGTGTGAAGGT+GGG 0.590711 6_1:+100056167 None:intergenic
AAGAAAATCAGCAAGGACAA+GGG 0.599976 6_1:+100058376 Msa0902220:CDS
CAGTTTACAGGAAACTCTCA+GGG 0.612531 6_1:+100059769 Msa0902220:CDS
TGAGAAGAAATTCAGCGGAA+AGG 0.621961 6_1:+100058800 Msa0902220:CDS
TGATGTTTCTGGAAACCGCA+AGG 0.622049 6_1:+100058704 Msa0902220:CDS
ATCCCCTACAGATTGAGGAA+GGG 0.622569 6_1:+100058741 Msa0902220:CDS
ACTGACTCCTCAAATTCAGT+AGG 0.622702 6_1:-100058406 None:intergenic
AGCCGAAGAAGATGCAGATG+CGG 0.627417 6_1:-100056345 None:intergenic
AGGGCGCGCGAGAAGTGTGA+AGG 0.630598 6_1:+100056163 None:intergenic
ACCCCTTCCTCAATCTGTAG+GGG 0.633929 6_1:-100058743 None:intergenic
TGATGGATCCAAGATCATGA+AGG 0.639271 6_1:+100059487 Msa0902220:CDS
TGTCCACGAGGCAATGCTCG+AGG 0.642990 6_1:+100059415 Msa0902220:CDS
TTCAAGGAAGAAAATCAGCA+AGG 0.648771 6_1:+100058369 Msa0902220:CDS
GCAGTGAGTGTACGGCTGCG+TGG 0.657285 6_1:-100059392 None:intergenic
TACACTCACTGCTGTCCACG+AGG 0.659160 6_1:+100059403 Msa0902220:CDS
TGGCCTGACGATTCTCCTGG+TGG 0.662830 6_1:-100059342 None:intergenic
ATGTGATATAACTTGTGTTG+CGG 0.669488 6_1:+100060060 Msa0902220:three_prime_UTR
TATGGATAACCACAGCCTTG+CGG 0.675676 6_1:-100058719 None:intergenic
GAATCTACGCCTCAGTAACA+GGG 0.678065 6_1:-100059812 None:intergenic
CATCCTCGAGCATTGCCTCG+TGG 0.690997 6_1:-100059418 None:intergenic
GAGCTTGAGAAGAAATTCAG+CGG 0.694607 6_1:+100058795 Msa0902220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATTTGAAATTTAAAAA+TGG + chr6_1:100056541-100056560 Msa0902220:intron 10.0%
!!! TTCATTTGAAATTTAAAAAT+GGG + chr6_1:100056542-100056561 Msa0902220:intron 10.0%
!!! GTAATTTTTATAAACTAAAT+TGG - chr6_1:100056830-100056849 None:intergenic 10.0%
!! TATAATAAATAATGTATCAT+AGG - chr6_1:100059949-100059968 None:intergenic 10.0%
!! TATGATACATTATTTATTAT+AGG + chr6_1:100059948-100059967 Msa0902220:three_prime_UTR 10.0%
!! TAAATGATATATTAAAATGA+CGG + chr6_1:100060084-100060103 Msa0902220:three_prime_UTR 10.0%
!! ATTGTTATAGAAATAGATAA+AGG + chr6_1:100056725-100056744 Msa0902220:intron 15.0%
!!! AAAAATCGTTTTTTATTGAT+TGG + chr6_1:100057736-100057755 Msa0902220:intron 15.0%
!! ATATGATGAATGTTTATTTA+TGG + chr6_1:100059872-100059891 Msa0902220:three_prime_UTR 15.0%
!! CAACACATATATTTACAAAA+TGG - chr6_1:100056228-100056247 None:intergenic 20.0%
!!! CATTTTGTAAATATATGTGT+TGG + chr6_1:100056226-100056245 Msa0902220:five_prime_UTR 20.0%
!!! TTGTTAACCTATATAGTTTT+GGG - chr6_1:100056264-100056283 None:intergenic 20.0%
!!! TTTGTTAACCTATATAGTTT+TGG - chr6_1:100056265-100056284 None:intergenic 20.0%
!! AAACTATATAGGTTAACAAA+CGG + chr6_1:100056265-100056284 Msa0902220:five_prime_UTR 20.0%
!! AACTTTGAATTCAATTAACA+TGG - chr6_1:100056431-100056450 None:intergenic 20.0%
!! ATGAAAACAGTTTATGATAA+AGG + chr6_1:100056795-100056814 Msa0902220:intron 20.0%
!! TGAAAACAGTTTATGATAAA+GGG + chr6_1:100056796-100056815 Msa0902220:intron 20.0%
!!! AAACGATTTAACTTAACTTT+GGG - chr6_1:100056955-100056974 None:intergenic 20.0%
!!! AAAACGATTTAACTTAACTT+TGG - chr6_1:100056956-100056975 None:intergenic 20.0%
!!! GTAGTTTTTATAAGCTATAT+TGG + chr6_1:100056990-100057009 Msa0902220:intron 20.0%
!!! ATTTTATCAGTGAATATGAT+TGG + chr6_1:100057285-100057304 Msa0902220:intron 20.0%
!!! TTTTCTTTTTGTTTTTTCCT+CGG + chr6_1:100057448-100057467 Msa0902220:intron 20.0%
!!! TATGCTTTTTTTTGTCTAAT+GGG + chr6_1:100057516-100057535 Msa0902220:intron 20.0%
!! TTTGAATCATTTGAGAAAAT+TGG + chr6_1:100057768-100057787 Msa0902220:intron 20.0%
!! TTGAATCATTTGAGAAAATT+GGG + chr6_1:100057769-100057788 Msa0902220:intron 20.0%
!! GAAACATTAACAAGAAAAAT+GGG - chr6_1:100057899-100057918 None:intergenic 20.0%
!! AGAAACATTAACAAGAAAAA+TGG - chr6_1:100057900-100057919 None:intergenic 20.0%
!!! TTTTTCAATATTTTGTTTCG+TGG + chr6_1:100057963-100057982 Msa0902220:intron 20.0%
!!! AGGTTATATATTGATTACTA+CGG + chr6_1:100058434-100058453 Msa0902220:intron 20.0%
!! AAATTGGAACAAAAAATTGT+CGG - chr6_1:100058897-100058916 None:intergenic 20.0%
!! AGAATAAAGTTCTTGAAATA+AGG - chr6_1:100058946-100058965 None:intergenic 20.0%
!! TTAAAGCAATTTAAAAACCA+AGG - chr6_1:100058981-100059000 None:intergenic 20.0%
!! CAAATTATTTAGTGTATGTT+TGG - chr6_1:100059211-100059230 None:intergenic 20.0%
!! TATTATGAAAAGTCTTTCTT+GGG - chr6_1:100060033-100060052 None:intergenic 20.0%
!! TTATTATGAAAAGTCTTTCT+TGG - chr6_1:100060034-100060053 None:intergenic 20.0%
!!! TGTTTTCCAACTTTTTTCTT+GGG - chr6_1:100056203-100056222 None:intergenic 25.0%
!!! ATGTTTTCCAACTTTTTTCT+TGG - chr6_1:100056204-100056223 None:intergenic 25.0%
! AACTATATAGGTTAACAAAC+GGG + chr6_1:100056266-100056285 Msa0902220:five_prime_UTR 25.0%
! TAGAGCAATAAATCGTAAAA+GGG - chr6_1:100056388-100056407 None:intergenic 25.0%
! ATAGAGCAATAAATCGTAAA+AGG - chr6_1:100056389-100056408 None:intergenic 25.0%
!!! TCTTTCTAAACCCTATTTTT+TGG + chr6_1:100056464-100056483 Msa0902220:intron 25.0%
! GATTGATATGGTTAATTACT+TGG + chr6_1:100056605-100056624 Msa0902220:intron 25.0%
! TAAATTAAGCTGCAAATAAG+TGG - chr6_1:100056685-100056704 None:intergenic 25.0%
! TAAATTGCTGTGTAACTATA+AGG + chr6_1:100057176-100057195 Msa0902220:intron 25.0%
!! ATACTTGACATTTTTATGCT+TGG + chr6_1:100057244-100057263 Msa0902220:intron 25.0%
!!! CTATGCTTTTTTTTGTCTAA+TGG + chr6_1:100057515-100057534 Msa0902220:intron 25.0%
! GAAATTTGTGATGTAATAGT+AGG + chr6_1:100057559-100057578 Msa0902220:intron 25.0%
! ACATATTACTATTGCTTCAA+GGG - chr6_1:100057635-100057654 None:intergenic 25.0%
! AAAACCAAAATAAAGGATTG+TGG - chr6_1:100057678-100057697 None:intergenic 25.0%
!!! ACAATCCTTTATTTTGGTTT+TGG + chr6_1:100057677-100057696 Msa0902220:intron 25.0%
!!! CAATCCTTTATTTTGGTTTT+GGG + chr6_1:100057678-100057697 Msa0902220:intron 25.0%
!! CAGATTTTATGAACATCTAA+GGG + chr6_1:100057852-100057871 Msa0902220:intron 25.0%
!! AGATTTTATGAACATCTAAG+GGG + chr6_1:100057853-100057872 Msa0902220:intron 25.0%
!!! GTTTCTGTTGTTTTTATGTT+TGG + chr6_1:100057914-100057933 Msa0902220:intron 25.0%
! TGCAATAATGATGTTGTATT+TGG + chr6_1:100058080-100058099 Msa0902220:intron 25.0%
! TTGTGAATATGTTTACTTCA+AGG + chr6_1:100058353-100058372 Msa0902220:exon 25.0%
! TATTTAGTGTATGTTTGGTT+TGG - chr6_1:100059206-100059225 None:intergenic 25.0%
!! TGTGAGAAAACAATTTTTCA+AGG - chr6_1:100059252-100059271 None:intergenic 25.0%
!!! AGTAACTTTTGGAAATTGTT+TGG - chr6_1:100059529-100059548 None:intergenic 25.0%
!!! TGCTTATTTTCACATTAGTT+TGG - chr6_1:100059585-100059604 None:intergenic 25.0%
!!! GATTCTATTTTTCTCAGTTT+TGG + chr6_1:100059830-100059849 Msa0902220:exon 25.0%
GAAATTCCCAAGAAAAAAGT+TGG + chr6_1:100056194-100056213 Msa0902220:five_prime_UTR 30.0%
!! TAAACCAAGAGTGTTTCATT+AGG + chr6_1:100056291-100056310 Msa0902220:five_prime_UTR 30.0%
!! AAACCAAGAGTGTTTCATTA+GGG + chr6_1:100056292-100056311 Msa0902220:five_prime_UTR 30.0%
CATCGTTGTACCAAAAAATA+GGG - chr6_1:100056477-100056496 None:intergenic 30.0%
TCATCGTTGTACCAAAAAAT+AGG - chr6_1:100056478-100056497 None:intergenic 30.0%
!!! TTTTGTTGCACTGATTGATA+TGG + chr6_1:100056593-100056612 Msa0902220:intron 30.0%
TGTAACCTAACATGAATCTT+AGG + chr6_1:100056639-100056658 Msa0902220:intron 30.0%
GTAACCTAACATGAATCTTA+GGG + chr6_1:100056640-100056659 Msa0902220:intron 30.0%
!!! GATAAAGGCTCTGTTTTTAT+TGG + chr6_1:100056740-100056759 Msa0902220:intron 30.0%
TTATACACAAGTGCTTATCA+TGG + chr6_1:100056886-100056905 Msa0902220:intron 30.0%
AATTTAGACTAAGGGCTATT+TGG - chr6_1:100057162-100057181 None:intergenic 30.0%
TACACAGCAATTTAGACTAA+GGG - chr6_1:100057170-100057189 None:intergenic 30.0%
TTACACAGCAATTTAGACTA+AGG - chr6_1:100057171-100057190 None:intergenic 30.0%
TGATAAAATACCACGATTAG+AGG - chr6_1:100057274-100057293 None:intergenic 30.0%
! ATCCTGACATGTTGAATTTT+AGG + chr6_1:100057384-100057403 Msa0902220:intron 30.0%
CACATATTACTATTGCTTCA+AGG - chr6_1:100057636-100057655 None:intergenic 30.0%
!! ACTTCCACAATCCTTTATTT+TGG + chr6_1:100057671-100057690 Msa0902220:intron 30.0%
TTCTCCCAAAACCAAAATAA+AGG - chr6_1:100057685-100057704 None:intergenic 30.0%
! GCAGATTTTATGAACATCTA+AGG + chr6_1:100057851-100057870 Msa0902220:intron 30.0%
!!! TGTTTTCAATTTGTTGTTGC+AGG + chr6_1:100058517-100058536 Msa0902220:intron 30.0%
TTAGAAAGAATAACTGGCAA+AGG - chr6_1:100058844-100058863 None:intergenic 30.0%
GACAGAATCGTAATAAAAGA+TGG - chr6_1:100058872-100058891 None:intergenic 30.0%
CTGTAAAACTGAAGTTAATC+AGG + chr6_1:100059032-100059051 Msa0902220:intron 30.0%
AACAAAAACTCACGATTACA+TGG + chr6_1:100059104-100059123 Msa0902220:intron 30.0%
CTAATGAACCTTAAACTTGT+AGG + chr6_1:100059304-100059323 Msa0902220:intron 30.0%
GATTACATCCTACAAGTTTA+AGG - chr6_1:100059315-100059334 None:intergenic 30.0%
ATAGTTTACCTTCATGATCT+TGG - chr6_1:100059498-100059517 None:intergenic 30.0%
!!! TTCAAGCTTTGAGTAACTTT+TGG - chr6_1:100059540-100059559 None:intergenic 30.0%
! TCTTGTGTTTCTGTGTATAT+AGG + chr6_1:100059689-100059708 Msa0902220:intron 30.0%
! TCTGTGTATATAGGTGTTTT+TGG + chr6_1:100059698-100059717 Msa0902220:intron 30.0%
!! CAGTATTGTTTTTCTCCTTT+GGG - chr6_1:100059725-100059744 None:intergenic 30.0%
!! TCAGTATTGTTTTTCTCCTT+TGG - chr6_1:100059726-100059745 None:intergenic 30.0%
CACATAGCAAAAAAGTCATT+TGG - chr6_1:100059976-100059995 None:intergenic 30.0%
!!! CAAATGACTTTTTTGCTATG+TGG + chr6_1:100059974-100059993 Msa0902220:three_prime_UTR 30.0%
! ATGTGATATAACTTGTGTTG+CGG + chr6_1:100060060-100060079 Msa0902220:three_prime_UTR 30.0%
!!! ATAGTTTTGGGCTTGTGTTT+GGG - chr6_1:100056252-100056271 None:intergenic 35.0%
!!! TATAGTTTTGGGCTTGTGTT+TGG - chr6_1:100056253-100056272 None:intergenic 35.0%
ACACAAGCCCAAAACTATAT+AGG + chr6_1:100056254-100056273 Msa0902220:five_prime_UTR 35.0%
AAACCCTAATGAAACACTCT+TGG - chr6_1:100056298-100056317 None:intergenic 35.0%
TAGGATTTGTGAGACAGTTT+AGG + chr6_1:100056765-100056784 Msa0902220:intron 35.0%
AACTTAACTTTGGGTCTGTT+TGG - chr6_1:100056946-100056965 None:intergenic 35.0%
AGCATAAACATCAACACACA+AGG - chr6_1:100057351-100057370 None:intergenic 35.0%
AGCCTAAAATTCAACATGTC+AGG - chr6_1:100057389-100057408 None:intergenic 35.0%
CTTAAACAATGCTTTAACCG+AGG - chr6_1:100057468-100057487 None:intergenic 35.0%
GCATTGTTTAAGTAGAAGCT+TGG + chr6_1:100057476-100057495 Msa0902220:intron 35.0%
TAATAGTAGGCTTCACTATC+TGG + chr6_1:100057572-100057591 Msa0902220:intron 35.0%
AACAGACCTGTAATGATTTG+CGG + chr6_1:100057820-100057839 Msa0902220:intron 35.0%
CAACATCATTATTGCAGTAG+TGG - chr6_1:100058076-100058095 None:intergenic 35.0%
ATGATGTTGTATTTGGACGT+CGG + chr6_1:100058087-100058106 Msa0902220:intron 35.0%
!!! ACACTTTTAGTTCTTCTGTC+TGG + chr6_1:100058153-100058172 Msa0902220:intron 35.0%
TCTGTCTGGTACTCAATATT+AGG + chr6_1:100058167-100058186 Msa0902220:intron 35.0%
CTGGTACTCAATATTAGGTT+TGG + chr6_1:100058172-100058191 Msa0902220:intron 35.0%
! TGCTGCTTATAGATCTTGTT+TGG + chr6_1:100058204-100058223 Msa0902220:intron 35.0%
CAATAAAAGCCTAAGAAGCA+AGG - chr6_1:100058261-100058280 None:intergenic 35.0%
TTCAAGGAAGAAAATCAGCA+AGG + chr6_1:100058369-100058388 Msa0902220:CDS 35.0%
AAGAAAATCAGCAAGGACAA+GGG + chr6_1:100058376-100058395 Msa0902220:CDS 35.0%
! ACTATTGTTGTTGCTGTGAT+TGG + chr6_1:100058457-100058476 Msa0902220:intron 35.0%
TACATCAATTCTGCAGTGTA+AGG + chr6_1:100058596-100058615 Msa0902220:intron 35.0%
GTGTAAGGATGTGTTCTTTA+TGG + chr6_1:100058611-100058630 Msa0902220:intron 35.0%
TGGCTGTAGATAACAGAAAA+CGG - chr6_1:100058681-100058700 None:intergenic 35.0%
GTGCAGTTAGAAAGAATAAC+TGG - chr6_1:100058850-100058869 None:intergenic 35.0%
CGAGTAGAAGACAAAGAAAT+TGG - chr6_1:100058913-100058932 None:intergenic 35.0%
! ATTCTGTTTTGCAATCTCCT+TGG + chr6_1:100058961-100058980 Msa0902220:intron 35.0%
GTCTAAGCATGTGTTTGATT+TGG + chr6_1:100059060-100059079 Msa0902220:intron 35.0%
GATAAAGAGAAGCTACAAAG+TGG - chr6_1:100059135-100059154 None:intergenic 35.0%
CGTACTAGATACAGAATTGA+TGG + chr6_1:100059470-100059489 Msa0902220:CDS 35.0%
! AAACGTTTTCTGCAGTTTAC+AGG + chr6_1:100059757-100059776 Msa0902220:CDS 35.0%
GTGGTTTATCCAATCCAAAC+AGG - chr6_1:100056666-100056685 None:intergenic 40.0%
!!! GGCTCTGTTTTTATTGGCAT+AGG + chr6_1:100056746-100056765 Msa0902220:intron 40.0%
! ACAGACCTGTAATGATTTGC+GGG + chr6_1:100057821-100057840 Msa0902220:intron 40.0%
TCATACCCGCAAATCATTAC+AGG - chr6_1:100057829-100057848 None:intergenic 40.0%
TGATGTTGTATTTGGACGTC+GGG + chr6_1:100058088-100058107 Msa0902220:intron 40.0%
TGTTTGGTACTAATACTGCG+TGG + chr6_1:100058220-100058239 Msa0902220:intron 40.0%
! TGATGATGACCTTGCTTCTT+AGG + chr6_1:100058249-100058268 Msa0902220:intron 40.0%
GAAGAAAATCAGCAAGGACA+AGG + chr6_1:100058375-100058394 Msa0902220:CDS 40.0%
! ACTGACTCCTCAAATTCAGT+AGG - chr6_1:100058409-100058428 None:intergenic 40.0%
ACTGAATTTGAGGAGTCAGT+TGG + chr6_1:100058409-100058428 Msa0902220:CDS 40.0%
!! TCAGATCACTTTTGAGCTCA+TGG - chr6_1:100058568-100058587 None:intergenic 40.0%
!! TACAGCCAAGTTGATGTTTC+TGG + chr6_1:100058693-100058712 Msa0902220:intron 40.0%
GGTTTCCAGAAACATCAACT+TGG - chr6_1:100058701-100058720 None:intergenic 40.0%
AAATTCACGTCAAGCTCGTT+AGG + chr6_1:100058772-100058791 Msa0902220:CDS 40.0%
AATTCACGTCAAGCTCGTTA+GGG + chr6_1:100058773-100058792 Msa0902220:CDS 40.0%
GAGCTTGAGAAGAAATTCAG+CGG + chr6_1:100058795-100058814 Msa0902220:CDS 40.0%
TGAGAAGAAATTCAGCGGAA+AGG + chr6_1:100058800-100058819 Msa0902220:CDS 40.0%
AACTGAAGTTAATCAGGCTG+CGG + chr6_1:100059038-100059057 Msa0902220:intron 40.0%
TGTTACCTGCTGAGATTGTT+GGG + chr6_1:100059444-100059463 Msa0902220:CDS 40.0%
! TGATGGATCCAAGATCATGA+AGG + chr6_1:100059487-100059506 Msa0902220:CDS 40.0%
!!! ATAGGTGTTTTTGGACCCAA+AGG + chr6_1:100059707-100059726 Msa0902220:intron 40.0%
CAGTTTACAGGAAACTCTCA+GGG + chr6_1:100059769-100059788 Msa0902220:CDS 40.0%
GTTGAAGCTCTCTATGATTC+TGG + chr6_1:100059904-100059923 Msa0902220:three_prime_UTR 40.0%
ATCTGCATCTTCTTCGGCTA+AGG + chr6_1:100056349-100056368 Msa0902220:five_prime_UTR 45.0%
CAGGCCCTAAGATTCATGTT+AGG - chr6_1:100056647-100056666 None:intergenic 45.0%
CATGAATCTTAGGGCCTGTT+TGG + chr6_1:100056649-100056668 Msa0902220:intron 45.0%
ATCTTAGGGCCTGTTTGGAT+TGG + chr6_1:100056654-100056673 Msa0902220:intron 45.0%
TCAAGGGAACGAGTCTGAAT+GGG - chr6_1:100057619-100057638 None:intergenic 45.0%
TTCAAGGGAACGAGTCTGAA+TGG - chr6_1:100057620-100057639 None:intergenic 45.0%
AGCGAGGGGAACACAATTAT+AGG - chr6_1:100058051-100058070 None:intergenic 45.0%
!! ATTGCAGTAGTGGATAGCGA+GGG - chr6_1:100058066-100058085 None:intergenic 45.0%
!! TATTGCAGTAGTGGATAGCG+AGG - chr6_1:100058067-100058086 None:intergenic 45.0%
ATTTGAGGAGTCAGTTGGAC+AGG + chr6_1:100058414-100058433 Msa0902220:CDS 45.0%
!! GTTGCAGGCTTTGTTTGATC+TGG + chr6_1:100058532-100058551 Msa0902220:intron 45.0%
!! TGATGTTTCTGGAAACCGCA+AGG + chr6_1:100058704-100058723 Msa0902220:CDS 45.0%
TATGGATAACCACAGCCTTG+CGG - chr6_1:100058722-100058741 None:intergenic 45.0%
TCCATATCCCCTACAGATTG+AGG + chr6_1:100058736-100058755 Msa0902220:CDS 45.0%
TCCTCAATCTGTAGGGGATA+TGG - chr6_1:100058740-100058759 None:intergenic 45.0%
TATCCCCTACAGATTGAGGA+AGG + chr6_1:100058740-100058759 Msa0902220:CDS 45.0%
ATCCCCTACAGATTGAGGAA+GGG + chr6_1:100058741-100058760 Msa0902220:CDS 45.0%
AACCCCTTCCTCAATCTGTA+GGG - chr6_1:100058747-100058766 None:intergenic 45.0%
! CGAGCTTGACGTGAATTTTG+CGG - chr6_1:100058770-100058789 None:intergenic 45.0%
ATGCCTTGTAATCATGCGAG+TGG + chr6_1:100059167-100059186 Msa0902220:intron 45.0%
TTGCCACTCGCATGATTACA+AGG - chr6_1:100059173-100059192 None:intergenic 45.0%
ATGTAATCCTCATTGCCACC+AGG + chr6_1:100059327-100059346 Msa0902220:CDS 45.0%
GTGTTACCTGCTGAGATTGT+TGG + chr6_1:100059443-100059462 Msa0902220:CDS 45.0%
GCAGTTTACAGGAAACTCTC+AGG + chr6_1:100059768-100059787 Msa0902220:CDS 45.0%
GTTTGAGTACCCTGTTACTG+AGG + chr6_1:100059803-100059822 Msa0902220:CDS 45.0%
GAATCTACGCCTCAGTAACA+GGG - chr6_1:100059815-100059834 None:intergenic 45.0%
AGAATCTACGCCTCAGTAAC+AGG - chr6_1:100059816-100059835 None:intergenic 45.0%
AGCCGCATCTGCATCTTCTT+CGG + chr6_1:100056343-100056362 Msa0902220:five_prime_UTR 50.0%
AGCCGAAGAAGATGCAGATG+CGG - chr6_1:100056348-100056367 None:intergenic 50.0%
GCTTGGATGTCCTCTAATCG+TGG + chr6_1:100057261-100057280 Msa0902220:intron 50.0%
GCTTCACTATCTGGTCGATG+CGG + chr6_1:100057581-100057600 Msa0902220:intron 50.0%
!! TTGCAGTAGTGGATAGCGAG+GGG - chr6_1:100058065-100058084 None:intergenic 50.0%
CGCTGAGCCTACTGAATTTG+AGG + chr6_1:100058399-100058418 Msa0902220:CDS 50.0%
TCCCCTACAGATTGAGGAAG+GGG + chr6_1:100058742-100058761 Msa0902220:CDS 50.0%
ACCCCTTCCTCAATCTGTAG+GGG - chr6_1:100058746-100058765 None:intergenic 50.0%
GAACCCCTTCCTCAATCTGT+AGG - chr6_1:100058748-100058767 None:intergenic 50.0%
ATCGTCAGGCCACCAAAGAA+AGG + chr6_1:100059353-100059372 Msa0902220:CDS 50.0%
CGTTTCCCAACAATCTCAGC+AGG - chr6_1:100059452-100059471 None:intergenic 50.0%
! TTCTGGAAACCGCAAGGCTG+TGG + chr6_1:100058710-100058729 Msa0902220:CDS 55.0%
CGATTCTCCTGGTGGCAATG+AGG - chr6_1:100059337-100059356 None:intergenic 55.0%
TTGCCACCAGGAGAATCGTC+AGG + chr6_1:100059339-100059358 Msa0902220:CDS 55.0%
TGAGCAGCGGAGCCTTTCTT+TGG - chr6_1:100059368-100059387 None:intergenic 55.0%
CGTGGACAGCAGTGAGTGTA+CGG - chr6_1:100059403-100059422 None:intergenic 55.0%
TACACTCACTGCTGTCCACG+AGG + chr6_1:100059403-100059422 Msa0902220:CDS 55.0%
TGGCCTGACGATTCTCCTGG+TGG - chr6_1:100059345-100059364 None:intergenic 60.0%
TGGTGGCCTGACGATTCTCC+TGG - chr6_1:100059348-100059367 None:intergenic 60.0%
GCAGCGGAGCCTTTCTTTGG+TGG - chr6_1:100059365-100059384 None:intergenic 60.0%
TGTCCACGAGGCAATGCTCG+AGG + chr6_1:100059415-100059434 Msa0902220:CDS 60.0%
! CATCCTCGAGCATTGCCTCG+TGG - chr6_1:100059421-100059440 None:intergenic 60.0%
GCAGTGAGTGTACGGCTGCG+TGG - chr6_1:100059395-100059414 None:intergenic 65.0%
GCTGCGTGGACGCTGAGCAG+CGG - chr6_1:100059381-100059400 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 100056170 100060115 100056170 ID=Msa0902220;Name=Msa0902220
chr6_1 mRNA 100056170 100060115 100056170 ID=Msa0902220-mRNA-1;Parent=Msa0902220;Name=Msa0902220-mRNA-1;_AED=0.04;_eAED=0.04;_QI=216|1|1|1|0.8|0.66|6|285|191
chr6_1 exon 100056170 100056370 100056170 ID=Msa0902220-mRNA-1:exon:17670;Parent=Msa0902220-mRNA-1
chr6_1 exon 100058346 100058435 100058346 ID=Msa0902220-mRNA-1:exon:17671;Parent=Msa0902220-mRNA-1
chr6_1 exon 100058539 100058612 100058539 ID=Msa0902220-mRNA-1:exon:17672;Parent=Msa0902220-mRNA-1
chr6_1 exon 100058698 100058821 100058698 ID=Msa0902220-mRNA-1:exon:17673;Parent=Msa0902220-mRNA-1
chr6_1 exon 100059326 100059508 100059326 ID=Msa0902220-mRNA-1:exon:17674;Parent=Msa0902220-mRNA-1
chr6_1 exon 100059711 100060115 100059711 ID=Msa0902220-mRNA-1:exon:17675;Parent=Msa0902220-mRNA-1
chr6_1 five_prime_UTR 100056170 100056370 100056170 ID=Msa0902220-mRNA-1:five_prime_utr;Parent=Msa0902220-mRNA-1
chr6_1 five_prime_UTR 100058346 100058360 100058346 ID=Msa0902220-mRNA-1:five_prime_utr;Parent=Msa0902220-mRNA-1
chr6_1 CDS 100058361 100058435 100058361 ID=Msa0902220-mRNA-1:cds;Parent=Msa0902220-mRNA-1
chr6_1 CDS 100058539 100058612 100058539 ID=Msa0902220-mRNA-1:cds;Parent=Msa0902220-mRNA-1
chr6_1 CDS 100058698 100058821 100058698 ID=Msa0902220-mRNA-1:cds;Parent=Msa0902220-mRNA-1
chr6_1 CDS 100059326 100059508 100059326 ID=Msa0902220-mRNA-1:cds;Parent=Msa0902220-mRNA-1
chr6_1 CDS 100059711 100059830 100059711 ID=Msa0902220-mRNA-1:cds;Parent=Msa0902220-mRNA-1
chr6_1 three_prime_UTR 100059831 100060115 100059831 ID=Msa0902220-mRNA-1:three_prime_utr;Parent=Msa0902220-mRNA-1
Gene Sequence

>Msa0902220

ATGTTTACTTCAAGGAAGAAAATCAGCAAGGACAAGGGCGCTGAGCCTACTGAATTTGAGGAGTCAGTTGGACAGGCTTTGTTTGATCTGGAAAACACTAACCATGAGCTCAAAAGTGATCTGAAAGATTTATACATCAATTCTGCAGTCCAAGTTGATGTTTCTGGAAACCGCAAGGCTGTGGTTATCCATATCCCCTACAGATTGAGGAAGGGGTTCCGCAAAATTCACGTCAAGCTCGTTAGGGAGCTTGAGAAGAAATTCAGCGGAAAGGATGTAATCCTCATTGCCACCAGGAGAATCGTCAGGCCACCAAAGAAAGGCTCCGCTGCTCAGCGTCCACGCAGCCGTACACTCACTGCTGTCCACGAGGCAATGCTCGAGGATGTCGTGTTACCTGCTGAGATTGTTGGGAAACGTACTAGATACAGAATTGATGGATCCAAGATCATGAAGGTGTTTTTGGACCCAAAGGAGAAAAACAATACTGAATACAAGCTCGAAACGTTTTCTGCAGTTTACAGGAAACTCTCAGGGAAAGATGTTGTGTTTGAGTACCCTGTTACTGAGGCGTAG
Protein sequence

>Msa0902220

MFTSRKKISKDKGAEPTEFEESVGQALFDLENTNHELKSDLKDLYINSAVQVDVSGNRKAVVIHIPYRLRKGFRKIHVKLVRELEKKFSGKDVILIATRRIVRPPKKGSAAQRPRSRTLTAVHEAMLEDVVLPAEIVGKRTRYRIDGSKIMKVFLDPKEKNNTEYKLETFSAVYRKLSGKDVVFEYPVTEA*