AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192750 MtrunA17_Chr2g0283551 100.000 86 0 0 1 86 1 86 1.11e-60 179
Msa0192750 MtrunA17_Chr2g0291901 98.837 86 1 0 1 86 1 86 3.27e-60 177
Msa0192750 MtrunA17_Chr1g0210581 98.837 86 1 0 1 86 1 86 3.27e-60 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192750 AT3G61110.1 95.349 86 4 0 1 86 1 86 4.63e-59 175
Msa0192750 AT2G45710.1 94.048 84 5 0 1 84 1 84 3.86e-56 167
Msa0192750 AT5G47930.1 95.238 84 4 0 1 84 1 84 1.13e-55 166
Msa0192750 AT3G61111.1 82.558 86 15 0 1 86 1 86 4.94e-49 150
Msa0192750 AT3G61111.2 80.952 42 8 0 1 42 1 42 1.06e-18 73.6

Find 37 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATGCTAGGTTAAAGTTT+TGG 0.280121 2_1:+8580482 None:intergenic
TCTATTTACTGTTGTGCATC+TGG 0.299569 2_1:-8580461 Msa0192750:three_prime_UTR
GTTGCAGAGTTGTGGTTGTT+AGG 0.336511 2_1:-8580592 Msa0192750:three_prime_UTR
TAGGAAGAAGGGAGACTAAA+TGG 0.336934 2_1:-8580639 Msa0192750:exon
TAACAGAAGGATGCTCCTTT+AGG 0.360674 2_1:-8580658 Msa0192750:CDS
TTTCTCTTCTCAAGCTCAGC+TGG 0.378351 2_1:+8581189 None:intergenic
ACCGTCTTGTGCCAGCCAAC+AGG 0.416778 2_1:-8580695 Msa0192750:CDS
TGTGGTTGTTAGGCTTAAGA+TGG 0.436847 2_1:-8580582 Msa0192750:three_prime_UTR
TTAGTCTCCCTTCTTCCTAA+AGG 0.438236 2_1:+8580643 None:intergenic
CTCTTCTCAAGCTCAGCTGG+TGG 0.449604 2_1:+8581192 None:intergenic
TGTCATCTTTACTTTGTCCA+TGG 0.464001 2_1:-8580614 Msa0192750:three_prime_UTR
TTGCACGGCCACCTGTTGGC+TGG 0.466909 2_1:+8580684 None:intergenic
CAACCACAACTCTGCAACCA+TGG 0.471278 2_1:+8580597 None:intergenic
AGCCAACAGGTGGCCGTGCA+AGG 0.485723 2_1:-8580682 Msa0192750:CDS
TCTTCTCAAGCTCAGCTGGT+GGG 0.492805 2_1:+8581193 None:intergenic
AGGATGCTCCTTTAGGAAGA+AGG 0.507022 2_1:-8580651 Msa0192750:CDS
GGATGCTCCTTTAGGAAGAA+GGG 0.515173 2_1:-8580650 Msa0192750:CDS
CTTACATATTGAAACAACCT+TGG 0.524068 2_1:+8581012 None:intergenic
TGTCCATGGTTGCAGAGTTG+TGG 0.535567 2_1:-8580600 Msa0192750:three_prime_UTR
AGCCTTGCACGGCCACCTGT+TGG 0.536283 2_1:+8580680 None:intergenic
CGTGTAATCTGTCTTGCGAT+TGG 0.550265 2_1:-8580343 Msa0192750:three_prime_UTR
TTGCAGGATGTAAAATGCCA+AGG 0.553924 2_1:-8581029 Msa0192750:intron
ATCCTTCTGTTAGCCTTGCA+CGG 0.562687 2_1:+8580669 None:intergenic
CAACAGTAAATAGAAATGCT+AGG 0.572412 2_1:+8580470 None:intergenic
ACCTGTTGGCTGGCACAAGA+CGG 0.579670 2_1:+8580694 None:intergenic
TAGCCACTCTCAAACCGTTG+TGG 0.599496 2_1:-8580735 Msa0192750:CDS
CAGGGCACTGACCATGTTGG+CGG 0.602656 2_1:+8581471 None:intergenic
GTCTTGTGCCAGCCAACAGG+TGG 0.604328 2_1:-8580692 Msa0192750:CDS
ACCATGTTGGCGGAAAATGT+TGG 0.615242 2_1:+8581481 None:intergenic
GGCCGTGCAAGGCTAACAGA+AGG 0.622759 2_1:-8580671 Msa0192750:CDS
TCTCAAACCGTTGTGGTGTG+CGG 0.635679 2_1:-8580728 Msa0192750:CDS
GAACAGGGCACTGACCATGT+TGG 0.640363 2_1:+8581468 None:intergenic
ACACCACAACGGTTTGAGAG+TGG 0.647039 2_1:+8580732 None:intergenic
CGGAAAATGTTGGAAAACAG+AGG 0.656697 2_1:+8581491 None:intergenic
TTGAGAGTGGCTAAAGACAG+TGG 0.660753 2_1:+8580745 None:intergenic
TCTTGCGATTGGTGTGTCCG+AGG 0.681893 2_1:-8580332 None:intergenic
ACAGTTACCGCACACCACAA+CGG 0.704309 2_1:+8580721 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTTTTATTGTTTTTTT+TGG - chr2_1:8580474-8580493 Msa0192750:three_prime_UTR 10.0%
!!! TTGATTTTTATTGTTTTTTT+TGG - chr2_1:8580474-8580493 Msa0192750:three_prime_UTR 10.0%
!!! TTTTGCAAAAATATCATTTA+TGG - chr2_1:8580449-8580468 Msa0192750:three_prime_UTR 15.0%
!! AAAAAGTTTATAAGCAAAAT+AGG + chr2_1:8581092-8581111 None:intergenic 15.0%
!!! TGCTTATAAACTTTTTATTT+TGG - chr2_1:8581096-8581115 Msa0192750:intron 15.0%
!!! TTTTGCAAAAATATCATTTA+TGG - chr2_1:8580449-8580468 Msa0192750:three_prime_UTR 15.0%
!! AAAAAGTTTATAAGCAAAAT+AGG + chr2_1:8581092-8581111 None:intergenic 15.0%
!!! TGCTTATAAACTTTTTATTT+TGG - chr2_1:8581096-8581115 Msa0192750:intron 15.0%
!!! ATCTCCTTCTGAAATTTTTA+GGG - chr2_1:8580338-8580357 Msa0192750:three_prime_UTR 25.0%
! AAAACCCTAAAAATTTCAGA+AGG + chr2_1:8580345-8580364 None:intergenic 25.0%
! CACAACATGAACATAAAATT+TGG + chr2_1:8580500-8580519 None:intergenic 25.0%
!!! AATCTATTTGTTGTTTGTTC+AGG - chr2_1:8580628-8580647 Msa0192750:exon 25.0%
! AATACCATGAAAAAAGAGTT+TGG + chr2_1:8580737-8580756 None:intergenic 25.0%
!!! TTGTTTTTCTCTTTTATTGC+AGG - chr2_1:8580827-8580846 Msa0192750:intron 25.0%
! ATAATAGTGCTCAAATTCAT+TGG - chr2_1:8580929-8580948 Msa0192750:intron 25.0%
!!! AAAGTTTTGGATAAGATTGT+GGG + chr2_1:8581380-8581399 None:intergenic 25.0%
!!! TAAAGTTTTGGATAAGATTG+TGG + chr2_1:8581381-8581400 None:intergenic 25.0%
!!! TCTTCATTATGTTTTGTTTG+TGG - chr2_1:8581506-8581525 Msa0192750:five_prime_UTR 25.0%
!!! ATCTCCTTCTGAAATTTTTA+GGG - chr2_1:8580338-8580357 Msa0192750:three_prime_UTR 25.0%
! AAAACCCTAAAAATTTCAGA+AGG + chr2_1:8580345-8580364 None:intergenic 25.0%
! CACAACATGAACATAAAATT+TGG + chr2_1:8580500-8580519 None:intergenic 25.0%
!!! AATCTATTTGTTGTTTGTTC+AGG - chr2_1:8580628-8580647 Msa0192750:exon 25.0%
! AATACCATGAAAAAAGAGTT+TGG + chr2_1:8580737-8580756 None:intergenic 25.0%
!!! TTGTTTTTCTCTTTTATTGC+AGG - chr2_1:8580827-8580846 Msa0192750:intron 25.0%
! ATAATAGTGCTCAAATTCAT+TGG - chr2_1:8580929-8580948 Msa0192750:intron 25.0%
!!! AAAGTTTTGGATAAGATTGT+GGG + chr2_1:8581380-8581399 None:intergenic 25.0%
!!! TAAAGTTTTGGATAAGATTG+TGG + chr2_1:8581381-8581400 None:intergenic 25.0%
!!! TCTTCATTATGTTTTGTTTG+TGG - chr2_1:8581506-8581525 Msa0192750:five_prime_UTR 25.0%
!!! CATCTCCTTCTGAAATTTTT+AGG - chr2_1:8580337-8580356 Msa0192750:three_prime_UTR 30.0%
CAAATTCAGATCTGAAAAAG+AGG + chr2_1:8580429-8580448 None:intergenic 30.0%
! TCATGTTGTGTTTTCATAAC+AGG - chr2_1:8580510-8580529 Msa0192750:three_prime_UTR 30.0%
CTTACATATTGAAACAACCT+TGG + chr2_1:8580863-8580882 None:intergenic 30.0%
! GGTTGTTTCAATATGTAAGT+TGG - chr2_1:8580864-8580883 Msa0192750:intron 30.0%
! GAAATGCTAGGTTAAAGTTT+TGG + chr2_1:8581393-8581412 None:intergenic 30.0%
CAACAGTAAATAGAAATGCT+AGG + chr2_1:8581405-8581424 None:intergenic 30.0%
!!! CATCTCCTTCTGAAATTTTT+AGG - chr2_1:8580337-8580356 Msa0192750:three_prime_UTR 30.0%
CAAATTCAGATCTGAAAAAG+AGG + chr2_1:8580429-8580448 None:intergenic 30.0%
! TCATGTTGTGTTTTCATAAC+AGG - chr2_1:8580510-8580529 Msa0192750:three_prime_UTR 30.0%
CTTACATATTGAAACAACCT+TGG + chr2_1:8580863-8580882 None:intergenic 30.0%
! GGTTGTTTCAATATGTAAGT+TGG - chr2_1:8580864-8580883 Msa0192750:intron 30.0%
! GAAATGCTAGGTTAAAGTTT+TGG + chr2_1:8581393-8581412 None:intergenic 30.0%
CAACAGTAAATAGAAATGCT+AGG + chr2_1:8581405-8581424 None:intergenic 30.0%
AGATCTGAAAAAGAGGAACA+GGG + chr2_1:8580422-8580441 None:intergenic 35.0%
!!! GTCACCAAACTCTTTTTTCA+TGG - chr2_1:8580730-8580749 Msa0192750:CDS 35.0%
TGTCATCTTTACTTTGTCCA+TGG - chr2_1:8581258-8581277 Msa0192750:intron 35.0%
TCTATTTACTGTTGTGCATC+TGG - chr2_1:8581411-8581430 Msa0192750:intron 35.0%
AGATCTGAAAAAGAGGAACA+GGG + chr2_1:8580422-8580441 None:intergenic 35.0%
!!! GTCACCAAACTCTTTTTTCA+TGG - chr2_1:8580730-8580749 Msa0192750:CDS 35.0%
TGTCATCTTTACTTTGTCCA+TGG - chr2_1:8581258-8581277 Msa0192750:intron 35.0%
TCTATTTACTGTTGTGCATC+TGG - chr2_1:8581411-8581430 Msa0192750:intron 35.0%
CGGAAAATGTTGGAAAACAG+AGG + chr2_1:8580384-8580403 None:intergenic 40.0%
CAGATCTGAAAAAGAGGAAC+AGG + chr2_1:8580423-8580442 None:intergenic 40.0%
TTGCAGGATGTAAAATGCCA+AGG - chr2_1:8580843-8580862 Msa0192750:intron 40.0%
TAACAGAAGGATGCTCCTTT+AGG - chr2_1:8581214-8581233 Msa0192750:CDS 40.0%
TTAGTCTCCCTTCTTCCTAA+AGG + chr2_1:8581232-8581251 None:intergenic 40.0%
TAGGAAGAAGGGAGACTAAA+TGG - chr2_1:8581233-8581252 Msa0192750:intron 40.0%
TGTGGTTGTTAGGCTTAAGA+TGG - chr2_1:8581290-8581309 Msa0192750:intron 40.0%
CGGAAAATGTTGGAAAACAG+AGG + chr2_1:8580384-8580403 None:intergenic 40.0%
CAGATCTGAAAAAGAGGAAC+AGG + chr2_1:8580423-8580442 None:intergenic 40.0%
TTGCAGGATGTAAAATGCCA+AGG - chr2_1:8580843-8580862 Msa0192750:intron 40.0%
TAACAGAAGGATGCTCCTTT+AGG - chr2_1:8581214-8581233 Msa0192750:CDS 40.0%
TTAGTCTCCCTTCTTCCTAA+AGG + chr2_1:8581232-8581251 None:intergenic 40.0%
TAGGAAGAAGGGAGACTAAA+TGG - chr2_1:8581233-8581252 Msa0192750:intron 40.0%
TGTGGTTGTTAGGCTTAAGA+TGG - chr2_1:8581290-8581309 Msa0192750:intron 40.0%
! TCCAACATTTTCCGCCAACA+TGG - chr2_1:8580390-8580409 Msa0192750:three_prime_UTR 45.0%
ACCATGTTGGCGGAAAATGT+TGG + chr2_1:8580394-8580413 None:intergenic 45.0%
TTTCTCTTCTCAAGCTCAGC+TGG + chr2_1:8580686-8580705 None:intergenic 45.0%
TTGAGAGTGGCTAAAGACAG+TGG + chr2_1:8581130-8581149 None:intergenic 45.0%
!! ATCCTTCTGTTAGCCTTGCA+CGG + chr2_1:8581206-8581225 None:intergenic 45.0%
! AGGATGCTCCTTTAGGAAGA+AGG - chr2_1:8581221-8581240 Msa0192750:CDS 45.0%
! GGATGCTCCTTTAGGAAGAA+GGG - chr2_1:8581222-8581241 Msa0192750:CDS 45.0%
GTTGCAGAGTTGTGGTTGTT+AGG - chr2_1:8581280-8581299 Msa0192750:intron 45.0%
CGTGTAATCTGTCTTGCGAT+TGG - chr2_1:8581529-8581548 Msa0192750:five_prime_UTR 45.0%
! TCCAACATTTTCCGCCAACA+TGG - chr2_1:8580390-8580409 Msa0192750:three_prime_UTR 45.0%
ACCATGTTGGCGGAAAATGT+TGG + chr2_1:8580394-8580413 None:intergenic 45.0%
TTTCTCTTCTCAAGCTCAGC+TGG + chr2_1:8580686-8580705 None:intergenic 45.0%
TTGAGAGTGGCTAAAGACAG+TGG + chr2_1:8581130-8581149 None:intergenic 45.0%
!! ATCCTTCTGTTAGCCTTGCA+CGG + chr2_1:8581206-8581225 None:intergenic 45.0%
! AGGATGCTCCTTTAGGAAGA+AGG - chr2_1:8581221-8581240 Msa0192750:CDS 45.0%
! GGATGCTCCTTTAGGAAGAA+GGG - chr2_1:8581222-8581241 Msa0192750:CDS 45.0%
GTTGCAGAGTTGTGGTTGTT+AGG - chr2_1:8581280-8581299 Msa0192750:intron 45.0%
CGTGTAATCTGTCTTGCGAT+TGG - chr2_1:8581529-8581548 Msa0192750:five_prime_UTR 45.0%
TCTTCTCAAGCTCAGCTGGT+GGG + chr2_1:8580682-8580701 None:intergenic 50.0%
TAGCCACTCTCAAACCGTTG+TGG - chr2_1:8581137-8581156 Msa0192750:intron 50.0%
ACACCACAACGGTTTGAGAG+TGG + chr2_1:8581143-8581162 None:intergenic 50.0%
TCTCAAACCGTTGTGGTGTG+CGG - chr2_1:8581144-8581163 Msa0192750:CDS 50.0%
ACAGTTACCGCACACCACAA+CGG + chr2_1:8581154-8581173 None:intergenic 50.0%
TGTCCATGGTTGCAGAGTTG+TGG - chr2_1:8581272-8581291 Msa0192750:intron 50.0%
CAACCACAACTCTGCAACCA+TGG + chr2_1:8581278-8581297 None:intergenic 50.0%
TCTTCTCAAGCTCAGCTGGT+GGG + chr2_1:8580682-8580701 None:intergenic 50.0%
TAGCCACTCTCAAACCGTTG+TGG - chr2_1:8581137-8581156 Msa0192750:intron 50.0%
ACACCACAACGGTTTGAGAG+TGG + chr2_1:8581143-8581162 None:intergenic 50.0%
TCTCAAACCGTTGTGGTGTG+CGG - chr2_1:8581144-8581163 Msa0192750:CDS 50.0%
ACAGTTACCGCACACCACAA+CGG + chr2_1:8581154-8581173 None:intergenic 50.0%
TGTCCATGGTTGCAGAGTTG+TGG - chr2_1:8581272-8581291 Msa0192750:intron 50.0%
CAACCACAACTCTGCAACCA+TGG + chr2_1:8581278-8581297 None:intergenic 50.0%
!! GAACAGGGCACTGACCATGT+TGG + chr2_1:8580407-8580426 None:intergenic 55.0%
CTCTTCTCAAGCTCAGCTGG+TGG + chr2_1:8580683-8580702 None:intergenic 55.0%
! ACCTGTTGGCTGGCACAAGA+CGG + chr2_1:8581181-8581200 None:intergenic 55.0%
!! GAACAGGGCACTGACCATGT+TGG + chr2_1:8580407-8580426 None:intergenic 55.0%
CTCTTCTCAAGCTCAGCTGG+TGG + chr2_1:8580683-8580702 None:intergenic 55.0%
! ACCTGTTGGCTGGCACAAGA+CGG + chr2_1:8581181-8581200 None:intergenic 55.0%
!! CAGGGCACTGACCATGTTGG+CGG + chr2_1:8580404-8580423 None:intergenic 60.0%
ACCGTCTTGTGCCAGCCAAC+AGG - chr2_1:8581177-8581196 Msa0192750:CDS 60.0%
GTCTTGTGCCAGCCAACAGG+TGG - chr2_1:8581180-8581199 Msa0192750:CDS 60.0%
GGCCGTGCAAGGCTAACAGA+AGG - chr2_1:8581201-8581220 Msa0192750:CDS 60.0%
!! CAGGGCACTGACCATGTTGG+CGG + chr2_1:8580404-8580423 None:intergenic 60.0%
ACCGTCTTGTGCCAGCCAAC+AGG - chr2_1:8581177-8581196 Msa0192750:CDS 60.0%
GTCTTGTGCCAGCCAACAGG+TGG - chr2_1:8581180-8581199 Msa0192750:CDS 60.0%
GGCCGTGCAAGGCTAACAGA+AGG - chr2_1:8581201-8581220 Msa0192750:CDS 60.0%
TTGCACGGCCACCTGTTGGC+TGG + chr2_1:8581191-8581210 None:intergenic 65.0%
AGCCAACAGGTGGCCGTGCA+AGG - chr2_1:8581190-8581209 Msa0192750:CDS 65.0%
AGCCTTGCACGGCCACCTGT+TGG + chr2_1:8581195-8581214 None:intergenic 65.0%
TTGCACGGCCACCTGTTGGC+TGG + chr2_1:8581191-8581210 None:intergenic 65.0%
AGCCAACAGGTGGCCGTGCA+AGG - chr2_1:8581190-8581209 Msa0192750:CDS 65.0%
AGCCTTGCACGGCCACCTGT+TGG + chr2_1:8581195-8581214 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8580333 8581561 8580333 ID=Msa0192750;Name=Msa0192750
chr2_1 mRNA 8580333 8581561 8580333 ID=Msa0192750-mRNA-1;Parent=Msa0192750;Name=Msa0192750-mRNA-1;_AED=0.03;_eAED=0.03;_QI=76|1|1|1|1|1|4|310|86
chr2_1 exon 8580333 8580769 8580333 ID=Msa0192750-mRNA-1:exon:3959;Parent=Msa0192750-mRNA-1
chr2_1 exon 8581017 8581045 8581017 ID=Msa0192750-mRNA-1:exon:3958;Parent=Msa0192750-mRNA-1
chr2_1 exon 8581143 8581244 8581143 ID=Msa0192750-mRNA-1:exon:3957;Parent=Msa0192750-mRNA-1
chr2_1 exon 8581483 8581561 8581483 ID=Msa0192750-mRNA-1:exon:3956;Parent=Msa0192750-mRNA-1
chr2_1 five_prime_UTR 8581486 8581561 8581486 ID=Msa0192750-mRNA-1:five_prime_utr;Parent=Msa0192750-mRNA-1
chr2_1 CDS 8581483 8581485 8581483 ID=Msa0192750-mRNA-1:cds;Parent=Msa0192750-mRNA-1
chr2_1 CDS 8581143 8581244 8581143 ID=Msa0192750-mRNA-1:cds;Parent=Msa0192750-mRNA-1
chr2_1 CDS 8581017 8581045 8581017 ID=Msa0192750-mRNA-1:cds;Parent=Msa0192750-mRNA-1
chr2_1 CDS 8580643 8580769 8580643 ID=Msa0192750-mRNA-1:cds;Parent=Msa0192750-mRNA-1
chr2_1 three_prime_UTR 8580333 8580642 8580333 ID=Msa0192750-mRNA-1:three_prime_utr;Parent=Msa0192750-mRNA-1
Gene Sequence

>Msa0192750

ATGGTTCTTCAAAATGATATTGATTTGTTAAACCCACCAGCTGAGCTTGAGAAGAGAAAGCACAAGCTCAAAAGACTTGTTCAGTCACCAAACTCTTTTTTCATGGATGTAAAATGCCAAGGTTGTTTCAATATAACCACTGTCTTTAGCCACTCTCAAACCGTTGTGGTGTGCGGTAACTGTCAGACCGTCTTGTGCCAGCCAACAGGTGGCCGTGCAAGGCTAACAGAAGGATGCTCCTTTAGGAAGAAGGGAGACTAA

Protein sequence

>Msa0192750

MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGRARLTEGCSFRKKGD*