AlfalfaGEDB | Msa0312210

M. sativa cultivar zhongmu-4 / Msa0312210

KEGG: K02898
GO: GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0015934 GO:0016020 GO:0019538 GO:0022613 GO:0022625 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0034645 GO:0042254 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0050896 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0098588 GO:0098805 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312210	XP_003593461.1	100.000	146	0	0	1	146	1	146	8.96e-100	293

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312210	sp\|Q9FJX2\|RL262_ARATH	88.356	146	17	0	1	146	1	146	1.02e-91	265

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027590	Msa0312210	0.831579	1.540886e-55	-8.615850e-47
Msa0032780	Msa0312210	0.852630	4.070462e-61	-8.615850e-47
Msa0068970	Msa0312210	0.802984	4.327685e-49	-8.615850e-47
Msa0075350	Msa0312210	0.818696	1.712953e-52	-8.615850e-47
Msa0087910	Msa0312210	0.816495	5.370956e-52	-8.615850e-47
Msa0114600	Msa0312210	0.831866	1.309049e-55	-8.615850e-47
Msa0134580	Msa0312210	0.828444	8.958480e-55	-8.615850e-47
Msa0167860	Msa0312210	0.843688	1.201719e-58	-8.615850e-47
Msa0186740	Msa0312210	0.850917	1.245321e-60	-8.615850e-47
Msa0191450	Msa0312210	0.824646	7.211628e-54	-8.615850e-47
Msa0191550	Msa0312210	0.840399	8.901278e-58	-8.615850e-47
Msa0194940	Msa0312210	0.874705	5.478670e-68	-8.615850e-47
Msa0210360	Msa0312210	0.835803	1.356219e-56	-8.615850e-47
Msa0222730	Msa0312210	0.837958	3.822002e-57	-8.615850e-47
Msa0229150	Msa0312210	0.802954	4.389871e-49	-8.615850e-47
Msa0235050	Msa0312210	0.809438	1.892291e-50	-8.615850e-47
Msa0277600	Msa0312210	0.814112	1.818776e-51	-8.615850e-47
Msa0281330	Msa0312210	0.836216	1.065385e-56	-8.615850e-47
Msa0281370	Msa0312210	0.836002	1.207459e-56	-8.615850e-47
Msa0312210	Msa0313520	0.803687	3.094900e-49	-8.615850e-47
Msa0312210	Msa0315430	0.832209	1.077208e-55	-8.615850e-47
Msa0312210	Msa0341880	0.832044	1.183302e-55	-8.615850e-47
Msa0312210	Msa0342850	0.828340	9.494214e-55	-8.615850e-47
Msa0312210	Msa0369410	0.802581	5.240990e-49	-8.615850e-47
Msa0312210	Msa0419710	0.825005	5.934404e-54	-8.615850e-47
Msa0312210	Msa0426090	0.828936	6.815217e-55	-8.615850e-47
Msa0312210	Msa0426250	0.850934	1.231156e-60	-8.615850e-47
Msa0312210	Msa0471880	0.816798	4.592217e-52	-8.615850e-47
Msa0312210	Msa0472480	0.819818	9.509636e-53	-8.615850e-47
Msa0312210	Msa0481520	0.814649	1.383231e-51	-8.615850e-47
Msa0312210	Msa0481850	0.842002	3.372396e-58	-8.615850e-47
Msa0312210	Msa0529900	0.839374	1.646446e-57	-8.615850e-47
Msa0312210	Msa0547920	0.805415	1.349677e-49	-8.615850e-47
Msa0312210	Msa0550420	0.824044	9.990463e-54	-8.615850e-47
Msa0312210	Msa0573550	0.836419	9.461215e-57	-8.615850e-47
Msa0312210	Msa0597240	0.806797	6.909418e-50	-8.615850e-47
Msa0312210	Msa0605890	0.851674	7.610750e-61	-8.615850e-47
Msa0312210	Msa0622230	0.827482	1.526796e-54	-8.615850e-47
Msa0312210	Msa0653060	0.820235	7.634201e-53	-8.615850e-47
Msa0312210	Msa0686550	0.810267	1.255125e-50	-8.615850e-47
Msa0312210	Msa0691330	0.803955	2.722253e-49	-8.615850e-47
Msa0312210	Msa0748350	0.832344	9.975737e-56	-8.615850e-47
Msa0312210	Msa0749000	0.814825	1.265060e-51	-8.615850e-47
Msa0312210	Msa0788380	0.820316	7.316259e-53	-8.615850e-47
Msa0312210	Msa0796490	0.808434	3.103041e-50	-8.615850e-47
Msa0312210	Msa0848240	0.800832	1.197772e-48	-8.615850e-47
Msa0312210	Msa0861320	0.801138	1.037132e-48	-8.615850e-47
Msa0312210	Msa0902220	0.806078	9.796975e-50	-8.615850e-47
Msa0312210	Msa0929700	0.830841	2.339724e-55	-8.615850e-47
Msa0312210	Msa1037290	0.815762	7.831037e-52	-8.615850e-47
Msa0312210	Msa1040160	0.829871	4.038183e-55	-8.615850e-47
Msa0312210	Msa1078980	0.810979	8.802634e-51	-8.615850e-47
Msa0312210	Msa1080350	0.842759	2.124706e-58	-8.615850e-47
Msa0312210	Msa1083370	0.825072	5.722855e-54	-8.615850e-47
Msa0312210	Msa1124850	0.816233	6.147016e-52	-8.615850e-47
Msa0312210	Msa1125860	0.839287	1.734211e-57	-8.615850e-47
Msa0312210	Msa1126060	0.839588	1.448509e-57	-8.615850e-47
Msa0312210	Msa1126520	0.825849	3.746254e-54	-8.615850e-47
Msa0312210	Msa1128120	0.810618	1.053951e-50	-8.615850e-47
Msa0312210	Msa1164150	0.815076	1.112334e-51	-8.615850e-47
Msa0312210	Msa1165640	0.827895	1.214926e-54	-8.615850e-47
Msa0312210	Msa1167540	0.834417	3.034399e-56	-8.615850e-47
Msa0312210	Msa1167550	0.804328	2.276960e-49	-8.615850e-47
Msa0312210	Msa1169180	0.806792	6.926375e-50	-8.615850e-47
Msa0312210	Msa1186030	0.853291	2.633437e-61	-8.615850e-47
Msa0312210	Msa1208330	0.803148	4.002632e-49	-8.615850e-47
Msa0312210	Msa1215820	0.802250	6.132037e-49	-8.615850e-47
Msa0312210	Msa1240270	0.814103	1.826942e-51	-8.615850e-47
Msa0312210	Msa1278130	0.804691	1.913014e-49	-8.615850e-47
Msa0312210	Msa1295150	0.816724	4.771869e-52	-8.615850e-47
Msa0312210	Msa1295170	0.803275	3.767194e-49	-8.615850e-47
Msa0312210	Msa1311400	0.847305	1.258097e-59	-8.615850e-47
Msa0312210	Msa1356790	0.804379	2.222592e-49	-8.615850e-47
Msa0312210	Msa1368920	0.816204	6.239356e-52	-8.615850e-47
Msa0312210	Msa1397100	0.811186	7.940484e-51	-8.615850e-47
Msa0312210	Msa1397660	0.800420	1.453320e-48	-8.615850e-47
Msa0312210	Msa1403910	0.800817	1.206312e-48	-8.615850e-47
Msa0312210	Msa1426120	0.803727	3.035857e-49	-8.615850e-47
Msa0312210	Msa1446410	0.832283	1.032551e-55	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312210	MtrunA17_Chr2g0281331	100.000	146	0	0	1	146	1	146	8.25e-104	293
Msa0312210	MtrunA17_Chr5g0401191	95.205	146	7	0	1	146	1	146	1.61e-90	259
Msa0312210	MtrunA17_Chr5g0447011	91.096	146	13	0	1	146	1	146	3.37e-87	251
Msa0312210	MtrunA17_Chr8g0370201	56.977	86	10	1	30	115	1	59	2.09e-23	87.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312210	AT5G67510.1	88.356	146	17	0	1	146	1	146	1.04e-92	265
Msa0312210	AT3G49910.1	85.616	146	21	0	1	146	1	146	1.23e-91	262

Find 50 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTATGCAGACCGTTGATT+AGG	0.363868	2_4:-7242200	None:intergenic
CATTGAACGGACGTTGTACT+TGG	0.377101	2_4:+7242501	None:intergenic
TCGCAAAGACGACGAAGTTC+AGG	0.379749	2_4:-7242473	Msa0312210:CDS
TTAGTGATAACAACCTTTGA+AGG	0.395167	2_4:+7242322	None:intergenic
ACTTCGTCGTCTTTGCGAAC+AGG	0.416424	2_4:+7242478	None:intergenic
ATGGATCTACCGTTAATGTT+GGG	0.416486	2_4:-7242351	Msa0312210:CDS
GGGTCGTGCTGCTGCTGATA+AGG	0.420559	2_4:-7242251	Msa0312210:CDS
ACGGCTCTTCCTACGGCTGC+TGG	0.422834	2_4:+7242597	None:intergenic
AAGACACGGCGGACGCTTGA+TGG	0.437229	2_4:+7242556	None:intergenic
CACTAAGCTCAGATTGGATA+AGG	0.438891	2_4:-7242305	Msa0312210:CDS
CCTGAGTAACCTTACCTTCA+CGG	0.446679	2_4:+7242428	None:intergenic
AGGATTAACCCCAACATTAA+CGG	0.448396	2_4:+7242342	None:intergenic
TGTTATCACTAAGCTCAGAT+TGG	0.466490	2_4:-7242311	Msa0312210:CDS
GACGAAGTTCAGGTCGTTCG+TGG	0.488796	2_4:-7242463	Msa0312210:CDS
ATTCAAAGGCCGTGAAGGTA+AGG	0.498862	2_4:-7242437	Msa0312210:CDS
CTCAGGTTTACCGTCGCAAG+TGG	0.512762	2_4:-7242411	Msa0312210:CDS
GCTTCTTGATCGTAAGGCTA+AGG	0.522703	2_4:-7242272	Msa0312210:CDS
TGTTGGGGTTAATCCTTCAA+AGG	0.524932	2_4:-7242335	Msa0312210:CDS
AATGGATCTACCGTTAATGT+TGG	0.529601	2_4:-7242352	Msa0312210:CDS
CTACGGCTGCTGGAAACTCG+TGG	0.530535	2_4:+7242607	None:intergenic
TGCTGCTGCTGATAAGGAGA+AGG	0.537077	2_4:-7242245	Msa0312210:CDS
GAGCCGTAAGGCTCATTTCA+CGG	0.545137	2_4:-7242581	Msa0312210:CDS
ATCACAAGGGAGAAGGTGAA+TGG	0.545267	2_4:-7242370	Msa0312210:CDS
GAGCCTTACGGCTCTTCCTA+CGG	0.547173	2_4:+7242590	None:intergenic
TAAATCGCTTCTTGATCGTA+AGG	0.549460	2_4:-7242278	Msa0312210:CDS
GTCGTTCGTGGAACATTCAA+AGG	0.554383	2_4:-7242451	Msa0312210:CDS
CTTCTTGATCGTAAGGCTAA+GGG	0.554681	2_4:-7242271	Msa0312210:CDS
GGGTACTAAGTTTGCTCCTG+AGG	0.563912	2_4:-7242224	Msa0312210:CDS
TGGATCTACCGTTAATGTTG+GGG	0.564613	2_4:-7242350	Msa0312210:CDS
AACGGACGTTGTACTTGGAT+CGG	0.564652	2_4:+7242506	None:intergenic
CCGTGAAGGTAAGGTTACTC+AGG	0.566699	2_4:-7242428	Msa0312210:CDS
CTTTGCGAACAGGCATTGAA+CGG	0.570326	2_4:+7242488	None:intergenic
GTTGTACTTGGATCGGAGAT+CGG	0.572814	2_4:+7242513	None:intergenic
ACGGTCTGCATAATATCCTC+AGG	0.575139	2_4:+7242208	None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG	0.575259	2_4:+7242578	None:intergenic
CGAACGCATCACAAGGGAGA+AGG	0.580140	2_4:-7242377	Msa0312210:CDS
GATCGGAGATCGGCGGAGAG+TGG	0.584970	2_4:+7242523	None:intergenic
TACGGCTGCTGGAAACTCGT+GGG	0.586325	2_4:+7242608	None:intergenic
TTCACATCGAACGCATCACA+AGG	0.588472	2_4:-7242384	Msa0312210:CDS
CAGCCGTAGGAAGAGCCGTA+AGG	0.591177	2_4:-7242593	Msa0312210:CDS
GGAACATTCAAAGGCCGTGA+AGG	0.606062	2_4:-7242442	Msa0312210:CDS
GATTCAAGAATCCTAATCAA+CGG	0.625900	2_4:+7242189	None:intergenic
GCTGCTGCTGATAAGGAGAA+GGG	0.634378	2_4:-7242244	Msa0312210:CDS
TGTGAATCACCCACTTGCGA+CGG	0.658219	2_4:+7242401	None:intergenic
TCAGGTTTACCGTCGCAAGT+GGG	0.670860	2_4:-7242410	Msa0312210:CDS
CACGAGTTTCCAGCAGCCGT+AGG	0.686122	2_4:-7242606	Msa0312210:CDS
GTGGCGCGCTCATTAAGACA+CGG	0.705562	2_4:+7242542	None:intergenic
GTACTTGGATCGGAGATCGG+CGG	0.709980	2_4:+7242516	None:intergenic
TCACATCGAACGCATCACAA+GGG	0.710980	2_4:-7242383	Msa0312210:CDS
GCGCGCTCATTAAGACACGG+CGG	0.823768	2_4:+7242545	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAGTGATAACAACCTTTGA+AGG	+	chr2_4:7242501-7242520	None:intergenic	30.0%
	AATGGATCTACCGTTAATGT+TGG	-	chr2_4:7242468-7242487	Msa0312210:CDS	35.0%
	ATGGATCTACCGTTAATGTT+GGG	-	chr2_4:7242469-7242488	Msa0312210:CDS	35.0%
	AGGATTAACCCCAACATTAA+CGG	+	chr2_4:7242481-7242500	None:intergenic	35.0%
	TGTTATCACTAAGCTCAGAT+TGG	-	chr2_4:7242509-7242528	Msa0312210:CDS	35.0%
	TAAATCGCTTCTTGATCGTA+AGG	-	chr2_4:7242542-7242561	Msa0312210:CDS	35.0%
	TGGATCTACCGTTAATGTTG+GGG	-	chr2_4:7242470-7242489	Msa0312210:CDS	40.0%
	TGTTGGGGTTAATCCTTCAA+AGG	-	chr2_4:7242485-7242504	Msa0312210:CDS	40.0%
	CACTAAGCTCAGATTGGATA+AGG	-	chr2_4:7242515-7242534	Msa0312210:CDS	40.0%
	CTTCTTGATCGTAAGGCTAA+GGG	-	chr2_4:7242549-7242568	Msa0312210:CDS	40.0%
	GTTGTACTTGGATCGGAGAT+CGG	+	chr2_4:7242310-7242329	None:intergenic	45.0%
	AACGGACGTTGTACTTGGAT+CGG	+	chr2_4:7242317-7242336	None:intergenic	45.0%
	CATTGAACGGACGTTGTACT+TGG	+	chr2_4:7242322-7242341	None:intergenic	45.0%
!!	CTTTGCGAACAGGCATTGAA+CGG	+	chr2_4:7242335-7242354	None:intergenic	45.0%
	GTCGTTCGTGGAACATTCAA+AGG	-	chr2_4:7242369-7242388	Msa0312210:CDS	45.0%
	ATTCAAAGGCCGTGAAGGTA+AGG	-	chr2_4:7242383-7242402	Msa0312210:CDS	45.0%
	CCTGAGTAACCTTACCTTCA+CGG	+	chr2_4:7242395-7242414	None:intergenic	45.0%
	TTCACATCGAACGCATCACA+AGG	-	chr2_4:7242436-7242455	Msa0312210:CDS	45.0%
	TCACATCGAACGCATCACAA+GGG	-	chr2_4:7242437-7242456	Msa0312210:CDS	45.0%
	ATCACAAGGGAGAAGGTGAA+TGG	-	chr2_4:7242450-7242469	Msa0312210:CDS	45.0%
	GCTTCTTGATCGTAAGGCTA+AGG	-	chr2_4:7242548-7242567	Msa0312210:CDS	45.0%
	ACGGTCTGCATAATATCCTC+AGG	+	chr2_4:7242615-7242634	None:intergenic	45.0%
	GAGCCGTAAGGCTCATTTCA+CGG	-	chr2_4:7242239-7242258	Msa0312210:CDS	50.0%
	ACTTCGTCGTCTTTGCGAAC+AGG	+	chr2_4:7242345-7242364	None:intergenic	50.0%
	TCGCAAAGACGACGAAGTTC+AGG	-	chr2_4:7242347-7242366	Msa0312210:CDS	50.0%
	GGAACATTCAAAGGCCGTGA+AGG	-	chr2_4:7242378-7242397	Msa0312210:CDS	50.0%
	CCGTGAAGGTAAGGTTACTC+AGG	-	chr2_4:7242392-7242411	Msa0312210:CDS	50.0%
	TCAGGTTTACCGTCGCAAGT+GGG	-	chr2_4:7242410-7242429	Msa0312210:CDS	50.0%
	TGTGAATCACCCACTTGCGA+CGG	+	chr2_4:7242422-7242441	None:intergenic	50.0%
	TGCTGCTGCTGATAAGGAGA+AGG	-	chr2_4:7242575-7242594	Msa0312210:CDS	50.0%
	GCTGCTGCTGATAAGGAGAA+GGG	-	chr2_4:7242576-7242595	Msa0312210:CDS	50.0%
!	GGGTACTAAGTTTGCTCCTG+AGG	-	chr2_4:7242596-7242615	Msa0312210:CDS	50.0%
	TACGGCTGCTGGAAACTCGT+GGG	+	chr2_4:7242215-7242234	None:intergenic	55.0%
	GAGCCTTACGGCTCTTCCTA+CGG	+	chr2_4:7242233-7242252	None:intergenic	55.0%
	GCGCCGTGAAATGAGCCTTA+CGG	+	chr2_4:7242245-7242264	None:intergenic	55.0%
	GTGGCGCGCTCATTAAGACA+CGG	+	chr2_4:7242281-7242300	None:intergenic	55.0%
	GTACTTGGATCGGAGATCGG+CGG	+	chr2_4:7242307-7242326	None:intergenic	55.0%
	GACGAAGTTCAGGTCGTTCG+TGG	-	chr2_4:7242357-7242376	Msa0312210:CDS	55.0%
	CTCAGGTTTACCGTCGCAAG+TGG	-	chr2_4:7242409-7242428	Msa0312210:CDS	55.0%
	CGAACGCATCACAAGGGAGA+AGG	-	chr2_4:7242443-7242462	Msa0312210:CDS	55.0%
	CTACGGCTGCTGGAAACTCG+TGG	+	chr2_4:7242216-7242235	None:intergenic	60.0%
	CACGAGTTTCCAGCAGCCGT+AGG	-	chr2_4:7242214-7242233	Msa0312210:CDS	60.0%
	CAGCCGTAGGAAGAGCCGTA+AGG	-	chr2_4:7242227-7242246	Msa0312210:CDS	60.0%
	AAGACACGGCGGACGCTTGA+TGG	+	chr2_4:7242267-7242286	None:intergenic	60.0%
	GCGCGCTCATTAAGACACGG+CGG	+	chr2_4:7242278-7242297	None:intergenic	60.0%
!	GGGTCGTGCTGCTGCTGATA+AGG	-	chr2_4:7242569-7242588	Msa0312210:CDS	60.0%
	ACGGCTCTTCCTACGGCTGC+TGG	+	chr2_4:7242226-7242245	None:intergenic	65.0%
	GATCGGAGATCGGCGGAGAG+TGG	+	chr2_4:7242300-7242319	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	7242201	7242641	7242201	ID=Msa0312210;Name=Msa0312210
chr2_4	mRNA	7242201	7242641	7242201	ID=Msa0312210-mRNA-1;Parent=Msa0312210;Name=Msa0312210-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|146
chr2_4	exon	7242201	7242641	7242201	ID=Msa0312210-mRNA-1:exon:2770;Parent=Msa0312210-mRNA-1
chr2_4	CDS	7242201	7242641	7242201	ID=Msa0312210-mRNA-1:cds;Parent=Msa0312210-mRNA-1

Gene Sequence

>Msa0312210

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCTTAATGAGCGCGCCACTCTCCGCCGATCTCCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAAGACGACGAAGTTCAGGTCGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAGGTTTACCGTCGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCTACCGTTAATGTTGGGGTTAATCCTTCAAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAATCGCTTCTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>Msa0312210

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD*