AlfalfaGEDB | Msa0186740

M. sativa cultivar zhongmu-4 / Msa0186740

KEGG: K02900
GO: GO:0000003 GO:0002181 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005911 GO:0006412 GO:0006518 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009791 GO:0009908 GO:0009987 GO:0010229 GO:0010467 GO:0015934 GO:0016020 GO:0019538 GO:0022414 GO:0022625 GO:0022626 GO:0030054 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034645 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0061458 GO:0070013 GO:0071704 GO:0090567 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00828.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186740	RHN68732.1	99.320	147	1	0	1	147	44	190	7.03e-101	297

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186740	sp\|P49637\|R27A3_ARATH	85.714	147	20	1	1	147	1	146	9.71e-88	255

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186740	A0A396IT43	99.320	147	1	0	1	147	44	190	3.36e-101	297

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027590	Msa0186740	0.810202	1.295800e-50	-8.615850e-47
Msa0044890	Msa0186740	0.812232	4.701297e-51	-8.615850e-47
Msa0068970	Msa0186740	0.810247	1.267106e-50	-8.615850e-47
Msa0167860	Msa0186740	0.800802	1.214913e-48	-8.615850e-47
Msa0186740	Msa0187810	0.802856	4.599898e-49	-8.615850e-47
Msa0186740	Msa0191450	0.819836	9.421799e-53	-8.615850e-47
Msa0186740	Msa0191550	0.820588	6.336789e-53	-8.615850e-47
Msa0186740	Msa0194940	0.869130	3.899263e-66	-8.615850e-47
Msa0186740	Msa0210360	0.829357	5.384804e-55	-8.615850e-47
Msa0186740	Msa0222730	0.831760	1.390148e-55	-8.615850e-47
Msa0186740	Msa0281330	0.838409	2.925386e-57	-8.615850e-47
Msa0186740	Msa0281370	0.822196	2.695677e-53	-8.615850e-47
Msa0186740	Msa0312210	0.850917	1.245321e-60	-8.615850e-47
Msa0186740	Msa0342850	0.855137	7.717301e-62	-8.615850e-47
Msa0186740	Msa0426090	0.819616	1.057981e-52	-8.615850e-47
Msa0186740	Msa0426250	0.828201	1.025329e-54	-8.615850e-47
Msa0186740	Msa0441310	0.820315	7.321125e-53	-8.615850e-47
Msa0186740	Msa0471880	0.847669	9.992741e-60	-8.615850e-47
Msa0186740	Msa0472480	0.811694	6.160403e-51	-8.615850e-47
Msa0186740	Msa0481520	0.805238	1.470403e-49	-8.615850e-47
Msa0186740	Msa0481850	0.807127	5.883612e-50	-8.615850e-47
Msa0186740	Msa0486070	0.803757	2.992660e-49	-8.615850e-47
Msa0186740	Msa0529900	0.808393	3.167044e-50	-8.615850e-47
Msa0186740	Msa0550420	0.839254	1.768468e-57	-8.615850e-47
Msa0186740	Msa0584030	0.838946	2.125960e-57	-8.615850e-47
Msa0186740	Msa0605890	0.816901	4.353582e-52	-8.615850e-47
Msa0186740	Msa0653060	0.810819	9.533249e-51	-8.615850e-47
Msa0186740	Msa0705800	0.805998	1.018087e-49	-8.615850e-47
Msa0186740	Msa0788380	0.812410	4.299250e-51	-8.615850e-47
Msa0186740	Msa0902220	0.823022	1.731892e-53	-8.615850e-47
Msa0186740	Msa0927910	0.818626	1.777255e-52	-8.615850e-47
Msa0186740	Msa1040160	0.826575	2.515839e-54	-8.615850e-47
Msa0186740	Msa1041680	0.807167	5.769124e-50	-8.615850e-47
Msa0186740	Msa1078870	0.811686	6.183763e-51	-8.615850e-47
Msa0186740	Msa1078980	0.812767	3.592910e-51	-8.615850e-47
Msa0186740	Msa1080350	0.819325	1.232613e-52	-8.615850e-47
Msa0186740	Msa1083370	0.819519	1.113288e-52	-8.615850e-47
Msa0186740	Msa1124850	0.823670	1.222549e-53	-8.615850e-47
Msa0186740	Msa1125860	0.813207	2.876174e-51	-8.615850e-47
Msa0186740	Msa1126060	0.816682	4.875363e-52	-8.615850e-47
Msa0186740	Msa1128120	0.833286	5.820252e-56	-8.615850e-47
Msa0186740	Msa1166570	0.803473	3.428108e-49	-8.615850e-47
Msa0186740	Msa1167550	0.832976	6.951223e-56	-8.615850e-47
Msa0186740	Msa1169180	0.803107	4.082663e-49	-8.615850e-47
Msa0186740	Msa1186030	0.826660	2.401885e-54	-8.615850e-47
Msa0186740	Msa1311400	0.843633	1.242474e-58	-8.615850e-47
Msa0186740	Msa1332250	0.807158	5.793979e-50	-8.615850e-47
Msa0186740	Msa1397100	0.843689	1.200787e-58	-8.615850e-47
Msa0186740	Msa1403910	0.833982	3.899415e-56	-8.615850e-47
Msa0186740	Msa1418770	0.807346	5.287074e-50	-8.615850e-47
Msa0186740	Msa1426120	0.819427	1.168356e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186740	MtrunA17_Chr3g0117071	99.320	147	1	0	1	147	44	190	6.46e-105	297
Msa0186740	MtrunA17_Chr5g0412761	100.000	147	0	0	1	147	1	147	2.36e-104	294
Msa0186740	MtrunA17_Chr8g0367991	84.459	148	22	1	1	147	1	148	5.45e-88	253
Msa0186740	MtrunA17_Chr4g0066961	48.182	110	8	2	38	147	1	61	2.92e-19	76.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186740	AT1G70600.1	85.714	147	20	1	1	147	1	146	9.87e-89	255
Msa0186740	AT1G23290.1	83.673	147	23	1	1	147	1	146	3.45e-86	248
Msa0186740	AT1G12960.1	50.000	146	27	2	1	146	1	100	1.81e-36	121

Find 46 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGATTGATGTTACTCAGTT+TGG	0.310495	2_1:-1509746	Msa0186740:CDS
TCTTCCTGTTCTTCTTGAAT+CGG	0.319606	2_1:+1510029	None:intergenic
TTTGATTCCACAAGATGTTA+AGG	0.375048	2_1:-1509798	Msa0186740:CDS
TGTTAAGGAGAAGGCTTTGA+AGG	0.376961	2_1:-1509783	Msa0186740:CDS
TGATTGATGTTACTCAGTTT+GGG	0.380111	2_1:-1509745	Msa0186740:CDS
GATTGCGGAGAAGAAGATTA+AGG	0.381172	2_1:-1509648	Msa0186740:CDS
CTTCCTGTTCTTCTTGAATC+GGG	0.382353	2_1:+1510030	None:intergenic
ATCTTGTCGATGTTGACAAT+GGG	0.394209	2_1:+1509827	None:intergenic
TCCGGCGTTACCACGACCTC+CGG	0.443108	2_1:+1509952	None:intergenic
CGCAAGCATCCCGGAGGTCG+TGG	0.449325	2_1:-1509962	Msa0186740:CDS
GCCTTCTCCTTAACATCTTG+TGG	0.452270	2_1:+1509791	None:intergenic
TGCTGTTGTTCTTACTGCTT+AGG	0.456887	2_1:-1509615	None:intergenic
AGCTTCGTCTTCACAACAAA+AGG	0.468189	2_1:+1509680	None:intergenic
TCCAGGTTACTTCGGTAAAG+TGG	0.475563	2_1:-1509891	Msa0186740:CDS
CCCACTTTACCGAAGTAACC+TGG	0.480893	2_1:+1509890	None:intergenic
CAACCCGATTCAAGAAGAAC+AGG	0.488410	2_1:-1510033	Msa0186740:CDS
TGGGGTTGTAGAATTTGTTA+CGG	0.498120	2_1:+1509846	None:intergenic
AAGCGCGGCCACGTCAGCGC+CGG	0.506311	2_1:-1510007	Msa0186740:CDS
GGCCACGTCAGCGCCGGACA+CGG	0.507873	2_1:-1510001	Msa0186740:CDS
GACCGTGTCCGGCGCTGACG+TGG	0.514645	2_1:+1509999	None:intergenic
AGCGCCGGACACGGTCGTAT+CGG	0.530105	2_1:-1509992	Msa0186740:CDS
TCCACAAGATGTTAAGGAGA+AGG	0.532638	2_1:-1509792	Msa0186740:CDS
CTCTTCGACAAGTACCATCC+AGG	0.549117	2_1:-1509908	Msa0186740:CDS
AGAATTTGTTACGGAGCTTG+TGG	0.563774	2_1:+1509855	None:intergenic
CACGACCTCCGGGATGCTTG+CGG	0.564581	2_1:+1509963	None:intergenic
CCCGGAGGTCGTGGTAACGC+CGG	0.572163	2_1:-1509953	Msa0186740:CDS
GGTACTTGTCGAAGAGGATG+CGG	0.572674	2_1:+1509915	None:intergenic
GAGAAGAAGATTAAGGAGGC+TGG	0.580387	2_1:-1509641	Msa0186740:CDS
GATCTTGTCGATGTTGACAA+TGG	0.583929	2_1:+1509826	None:intergenic
CCGGCGTTACCACGACCTCC+GGG	0.586499	2_1:+1509953	None:intergenic
GGAAAACACCGCAAGCATCC+CGG	0.587466	2_1:-1509971	Msa0186740:CDS
CCAGGTTACTTCGGTAAAGT+GGG	0.594593	2_1:-1509890	Msa0186740:CDS
AAGTACCATCCAGGTTACTT+CGG	0.598039	2_1:-1509899	Msa0186740:CDS
GAAGCTGATTTCGAAGATTG+CGG	0.600062	2_1:-1509663	Msa0186740:CDS
GCGGTGGTGATGCATACCTC+CGG	0.601360	2_1:+1509934	None:intergenic
CTGGATGGTACTTGTCGAAG+AGG	0.607823	2_1:+1509909	None:intergenic
GGAGGTCGTGGTAACGCCGG+AGG	0.608528	2_1:-1509950	Msa0186740:CDS
AACTGAGTAACATCAATCAC+CGG	0.615568	2_1:+1509749	None:intergenic
TTTGAAGGAGAACAAAGCGC+CGG	0.644488	2_1:-1509768	Msa0186740:CDS
CTTTACCGAAGTAACCTGGA+TGG	0.684945	2_1:+1509894	None:intergenic
AAGAAGATTAAGGAGGCTGG+TGG	0.689384	2_1:-1509638	Msa0186740:CDS
AAGAAGAACAGGAAGAAGCG+CGG	0.691929	2_1:-1510022	Msa0186740:CDS
TGCGGAGAAGAAGATTAAGG+AGG	0.697175	2_1:-1509645	Msa0186740:CDS
ACTTGTCGAAGAGGATGCGG+TGG	0.709791	2_1:+1509918	None:intergenic
TCTTGTCGATGTTGACAATG+GGG	0.721275	2_1:+1509828	None:intergenic
AAACACCGCAAGCATCCCGG+AGG	0.740520	2_1:-1509968	Msa0186740:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGGGTTTTTTAAGCTTTT+AGG	-	chr2_1:1509925-1509944	Msa0186740:CDS	25.0%
!!!	TTGGGTTTTTTAAGCTTTTA+GGG	-	chr2_1:1509926-1509945	Msa0186740:CDS	25.0%
!!!	TTTTTTAAGCTTTTAGGGAA+AGG	-	chr2_1:1509931-1509950	Msa0186740:CDS	25.0%
	TTTGATTCCACAAGATGTTA+AGG	-	chr2_1:1509855-1509874	Msa0186740:CDS	30.0%
	TGATTGATGTTACTCAGTTT+GGG	-	chr2_1:1509908-1509927	Msa0186740:CDS	30.0%
	TCTTCCTGTTCTTCTTGAAT+CGG	+	chr2_1:1509627-1509646	None:intergenic	35.0%
!!	TGGGGTTGTAGAATTTGTTA+CGG	+	chr2_1:1509810-1509829	None:intergenic	35.0%
	ATCTTGTCGATGTTGACAAT+GGG	+	chr2_1:1509829-1509848	None:intergenic	35.0%
	AACTGAGTAACATCAATCAC+CGG	+	chr2_1:1509907-1509926	None:intergenic	35.0%
	GTGATTGATGTTACTCAGTT+TGG	-	chr2_1:1509907-1509926	Msa0186740:CDS	35.0%
!!	ACAACAAAAGGCTGATTTTG+CGG	+	chr2_1:1509964-1509983	None:intergenic	35.0%
	CTTCCTGTTCTTCTTGAATC+GGG	+	chr2_1:1509626-1509645	None:intergenic	40.0%
	AAGTACCATCCAGGTTACTT+CGG	-	chr2_1:1509754-1509773	Msa0186740:CDS	40.0%
!!	AGAATTTGTTACGGAGCTTG+TGG	+	chr2_1:1509801-1509820	None:intergenic	40.0%
	TCTTGTCGATGTTGACAATG+GGG	+	chr2_1:1509828-1509847	None:intergenic	40.0%
	GATCTTGTCGATGTTGACAA+TGG	+	chr2_1:1509830-1509849	None:intergenic	40.0%
	TCCACAAGATGTTAAGGAGA+AGG	-	chr2_1:1509861-1509880	Msa0186740:CDS	40.0%
!	TGTTAAGGAGAAGGCTTTGA+AGG	-	chr2_1:1509870-1509889	Msa0186740:CDS	40.0%
	AGCTTCGTCTTCACAACAAA+AGG	+	chr2_1:1509976-1509995	None:intergenic	40.0%
!	GAAGCTGATTTCGAAGATTG+CGG	-	chr2_1:1509990-1510009	Msa0186740:CDS	40.0%
	GATTGCGGAGAAGAAGATTA+AGG	-	chr2_1:1510005-1510024	Msa0186740:CDS	40.0%
	CAACCCGATTCAAGAAGAAC+AGG	-	chr2_1:1509620-1509639	Msa0186740:CDS	45.0%
	AAGAAGAACAGGAAGAAGCG+CGG	-	chr2_1:1509631-1509650	Msa0186740:CDS	45.0%
	CTTTACCGAAGTAACCTGGA+TGG	+	chr2_1:1509762-1509781	None:intergenic	45.0%
	TCCAGGTTACTTCGGTAAAG+TGG	-	chr2_1:1509762-1509781	Msa0186740:CDS	45.0%
	CCAGGTTACTTCGGTAAAGT+GGG	-	chr2_1:1509763-1509782	Msa0186740:CDS	45.0%
	GCCTTCTCCTTAACATCTTG+TGG	+	chr2_1:1509865-1509884	None:intergenic	45.0%
	TTTGAAGGAGAACAAAGCGC+CGG	-	chr2_1:1509885-1509904	Msa0186740:CDS	45.0%
	TGCGGAGAAGAAGATTAAGG+AGG	-	chr2_1:1510008-1510027	Msa0186740:CDS	45.0%
	GAGAAGAAGATTAAGGAGGC+TGG	-	chr2_1:1510012-1510031	Msa0186740:CDS	45.0%
	AAGAAGATTAAGGAGGCTGG+TGG	-	chr2_1:1510015-1510034	Msa0186740:CDS	45.0%
!	TTTTCCGATACGACCGTGTC+CGG	+	chr2_1:1509668-1509687	None:intergenic	50.0%
	GGTACTTGTCGAAGAGGATG+CGG	+	chr2_1:1509741-1509760	None:intergenic	50.0%
	CTGGATGGTACTTGTCGAAG+AGG	+	chr2_1:1509747-1509766	None:intergenic	50.0%
	CTCTTCGACAAGTACCATCC+AGG	-	chr2_1:1509745-1509764	Msa0186740:CDS	50.0%
	CCCACTTTACCGAAGTAACC+TGG	+	chr2_1:1509766-1509785	None:intergenic	50.0%
	GGAAAACACCGCAAGCATCC+CGG	-	chr2_1:1509682-1509701	Msa0186740:CDS	55.0%
	ACTTGTCGAAGAGGATGCGG+TGG	+	chr2_1:1509738-1509757	None:intergenic	55.0%
	AAACACCGCAAGCATCCCGG+AGG	-	chr2_1:1509685-1509704	Msa0186740:CDS	60.0%
!	GCGGTGGTGATGCATACCTC+CGG	+	chr2_1:1509722-1509741	None:intergenic	60.0%
!	AGCGCCGGACACGGTCGTAT+CGG	-	chr2_1:1509661-1509680	Msa0186740:CDS	65.0%
	CACGACCTCCGGGATGCTTG+CGG	+	chr2_1:1509693-1509712	None:intergenic	65.0%
	TCCGGCGTTACCACGACCTC+CGG	+	chr2_1:1509704-1509723	None:intergenic	65.0%
	CGCAAGCATCCCGGAGGTCG+TGG	-	chr2_1:1509691-1509710	Msa0186740:CDS	70.0%
	CCGGCGTTACCACGACCTCC+GGG	+	chr2_1:1509703-1509722	None:intergenic	70.0%
!	CCCGGAGGTCGTGGTAACGC+CGG	-	chr2_1:1509700-1509719	Msa0186740:CDS	70.0%
!	GGAGGTCGTGGTAACGCCGG+AGG	-	chr2_1:1509703-1509722	Msa0186740:CDS	70.0%
	AAGCGCGGCCACGTCAGCGC+CGG	-	chr2_1:1509646-1509665	Msa0186740:CDS	75.0%
!	GGCCACGTCAGCGCCGGACA+CGG	-	chr2_1:1509652-1509671	Msa0186740:CDS	75.0%
!!	GACCGTGTCCGGCGCTGACG+TGG	+	chr2_1:1509657-1509676	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	1509616	1510059	1509616	ID=Msa0186740;Name=Msa0186740
chr2_1	mRNA	1509616	1510059	1509616	ID=Msa0186740-mRNA-1;Parent=Msa0186740;Name=Msa0186740-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|147
chr2_1	exon	1509616	1510059	1509616	ID=Msa0186740-mRNA-1:exon:673;Parent=Msa0186740-mRNA-1
chr2_1	CDS	1509616	1510059	1509616	ID=Msa0186740-mRNA-1:cds;Parent=Msa0186740-mRNA-1

Gene Sequence

>Msa0186740

ATGACAACCCGATTCAAGAAGAACAGGAAGAAGCGCGGCCACGTCAGCGCCGGACACGGTCGTATCGGAAAACACCGCAAGCATCCCGGAGGTCGTGGTAACGCCGGAGGTATGCATCACCACCGCATCCTCTTCGACAAGTACCATCCAGGTTACTTCGGTAAAGTGGGTATGCGTTACTTCCACAAGCTCCGTAACAAATTCTACAACCCCATTGTCAACATCGACAAGATCGCTTCTTTGATTCCACAAGATGTTAAGGAGAAGGCTTTGAAGGAGAACAAAGCGCCGGTGATTGATGTTACTCAGTTTGGGTTTTTTAAGCTTTTAGGGAAAGGTGTTTTGCCGCAAAATCAGCCTTTTGTTGTGAAGACGAAGCTGATTTCGAAGATTGCGGAGAAGAAGATTAAGGAGGCTGGTGGTGCTGTTGTTCTTACTGCTTAG

Protein sequence

>Msa0186740

MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKENKAPVIDVTQFGFFKLLGKGVLPQNQPFVVKTKLISKIAEKKIKEAGGAVVLTA*