AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1109390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1109390 MtrunA17_Chr7g0243541 97.175 177 5 0 1 177 233 409 7.12e-123 352
Msa1109390 MtrunA17_Chr4g0004981 86.957 161 21 0 1 161 286 446 2.09e-100 296
Msa1109390 MtrunA17_Chr2g0293201 42.515 167 91 3 2 167 306 468 3.36e-39 139
Msa1109390 MtrunA17_Chr8g0385291 47.712 153 75 4 5 156 346 494 1.47e-38 137
Msa1109390 MtrunA17_Chr4g0014901 46.405 153 79 3 5 156 437 587 8.82e-38 136
Msa1109390 MtrunA17_Chr4g0014911 39.053 169 93 5 2 164 301 465 1.46e-34 126
Msa1109390 MtrunA17_Chr3g0131071 44.444 144 76 3 5 147 416 556 3.07e-32 120
Msa1109390 MtrunA17_Chr1g0183881 33.784 148 95 1 5 152 313 457 1.67e-26 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1109390 AT3G51670.1 80.745 161 31 0 1 161 244 404 1.82e-93 278
Msa1109390 AT1G30690.1 44.937 158 81 3 2 157 379 532 8.32e-37 133
Msa1109390 AT1G30690.2 44.937 158 81 3 2 157 379 532 8.32e-37 133
Msa1109390 AT1G72160.1 42.683 164 88 5 1 160 327 488 3.19e-34 126
Msa1109390 AT4G09160.2 43.030 165 84 5 2 160 478 638 2.21e-32 122
Msa1109390 AT4G09160.3 43.030 165 84 5 2 160 478 638 2.46e-32 122
Msa1109390 AT4G09160.1 43.030 165 84 5 2 160 503 663 2.49e-32 122
Msa1109390 AT1G22530.2 42.405 158 81 6 3 156 493 644 1.39e-28 111
Msa1109390 AT1G22530.1 42.405 158 81 6 3 156 527 678 1.57e-28 111
Msa1109390 AT1G72150.1 36.943 157 92 5 3 157 419 570 2.13e-25 102

Find 110 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCTGAGTTCACTGTTAA+AGG 0.184177 7_3:-52929406 Msa1109390:CDS
GTGAACTCAGAAGCTGGTTT+TGG 0.239830 7_3:+52929415 None:intergenic
GCTAATCCGTGCTTCAATAT+TGG 0.243617 7_3:+52930113 None:intergenic
GCTAATCCGTGCTTCAATAT+TGG 0.243617 7_3:+52930281 None:intergenic
TTCCATTAATGTTGTGACTA+TGG 0.244429 7_3:+52931065 None:intergenic
AACCATAGTCACAACATTAA+TGG 0.252522 7_3:-52931067 Msa1109390:five_prime_UTR
TTTGAGGTTGGAAATGAAAT+AGG 0.258263 7_3:+52931005 None:intergenic
ACCTTACGAGCAACCATTTC+AGG 0.266158 7_3:+52930433 None:intergenic
CGGTCGATAACTCTGCTTCT+AGG 0.275710 7_3:-52928483 Msa1109390:CDS
CTTTCTTGGAGGAAAGAATT+TGG 0.299122 7_3:-52930793 Msa1109390:five_prime_UTR
TGCTTGTGAAGTGTCTTTCT+TGG 0.302342 7_3:-52930807 Msa1109390:five_prime_UTR
AAATGTTGTGGATGAAGATT+TGG 0.311161 7_3:-52930764 Msa1109390:five_prime_UTR
TGTAGTTGGAGGCTGGGATT+TGG 0.312615 7_3:-52928641 Msa1109390:CDS
CTTTCTTCTCTGAGGATTTG+AGG 0.314986 7_3:+52930989 None:intergenic
CTAATCCGTGCTTCAATATT+GGG 0.328301 7_3:+52930114 None:intergenic
CTAATCCGTGCTTCAATATT+GGG 0.328301 7_3:+52930282 None:intergenic
GTGTAGCTAGCTTCTGCATT+TGG 0.328696 7_3:+52928598 None:intergenic
GATTCAAGTTACTGATCTTA+AGG 0.332587 7_3:-52930521 Msa1109390:five_prime_UTR
GAGAATATTCCTATTCAATA+TGG 0.337092 7_3:-52929475 Msa1109390:CDS
GTGGTGTATTAGTACTTGTT+TGG 0.360457 7_3:-52928297 Msa1109390:three_prime_UTR
TTCCATGCAGAAATTTCATC+TGG 0.363571 7_3:-52931211 Msa1109390:five_prime_UTR
TTAGTTCTTTGAGTCAGAAA+TGG 0.368588 7_3:+52929794 None:intergenic
AAGGAAAAGCTCTTAACTTC+TGG 0.387606 7_3:-52930952 Msa1109390:five_prime_UTR
TTTCTTGGAGTTTCTAGAAA+TGG 0.388074 7_3:+52931114 None:intergenic
ACAACCCTTAACTCACTCTT+AGG 0.396909 7_3:+52930493 None:intergenic
GAGCAAGTTTGTGATCTCTA+AGG 0.402946 7_3:-52929771 Msa1109390:CDS
TCAAGATAACTATCCTGAAA+TGG 0.404693 7_3:-52930446 Msa1109390:five_prime_UTR
GGGAGATTGTAGTTGGAGGC+TGG 0.407967 7_3:-52928648 Msa1109390:CDS
ACTTGTTTGGTTTATGATGT+AGG 0.408578 7_3:-52928284 Msa1109390:three_prime_UTR
AGCAGGCATAATGGTACTTT+CGG 0.414858 7_3:-52928503 Msa1109390:CDS
AATTCCTCATTCACCATCAA+TGG 0.429468 7_3:-52931178 Msa1109390:five_prime_UTR
TTTCTAGAAATGGTGTGCTT+AGG 0.436314 7_3:+52931124 None:intergenic
GAGTGATCTTCTTCCATTGA+TGG 0.440321 7_3:+52931165 None:intergenic
AACAAAAGAAGCAGGCATAA+TGG 0.443455 7_3:-52928512 Msa1109390:CDS
AATGTTGTGGATGAAGATTT+GGG 0.445253 7_3:-52930763 Msa1109390:five_prime_UTR
ATGAAATTTCTGCATGGAAA+TGG 0.446188 7_3:+52931215 None:intergenic
CTCAATCCACCATATTGAAT+AGG 0.453387 7_3:+52929466 None:intergenic
CTTCTCTGAGGATTTGAGGT+TGG 0.455808 7_3:+52930993 None:intergenic
TCCTGAAATGGTTGCTCGTA+AGG 0.456947 7_3:-52930434 Msa1109390:five_prime_UTR
GGAGATTGTAGTTGGAGGCT+GGG 0.463853 7_3:-52928647 Msa1109390:CDS
AAAGTGAACATACAAATAGA+AGG 0.473146 7_3:-52929376 Msa1109390:CDS
TCTTCCATTGATGGTGAATG+AGG 0.473222 7_3:+52931174 None:intergenic
ATATGCCTAAGAGTGAGTTA+AGG 0.474991 7_3:-52930498 Msa1109390:five_prime_UTR
CCTGTGTGTTATAATCATTA+TGG 0.475087 7_3:-52930682 Msa1109390:five_prime_UTR
ATCAAAGAATTCACCCCACC+AGG 0.485860 7_3:+52930541 None:intergenic
AATCGAGTGATTATAGTCAT+TGG 0.488390 7_3:-52928131 Msa1109390:three_prime_UTR
CTACACTATAGAAGTTGAAA+AGG 0.488723 7_3:-52928581 Msa1109390:CDS
TATGCCTAAGAGTGAGTTAA+GGG 0.489546 7_3:-52930497 Msa1109390:five_prime_UTR
TTGTGAAGTGTCTTTCTTGG+AGG 0.498038 7_3:-52930804 Msa1109390:five_prime_UTR
ATCAAGTGGGAGATTGTAGT+TGG 0.499306 7_3:-52928655 Msa1109390:CDS
GAAAGCATTGAAACAGTTGA+AGG 0.500192 7_3:-52930971 Msa1109390:five_prime_UTR
TTATGGTGTGTTCAAAGATA+AGG 0.503248 7_3:-52930665 Msa1109390:five_prime_UTR
TACTCATGGGTTTGATAGAG+AGG 0.507545 7_3:-52930710 Msa1109390:five_prime_UTR
TTGGGATTCAAAGAGCTTGA+AGG 0.513195 7_3:-52930745 Msa1109390:five_prime_UTR
TTAACAGTGAACTCAGAAGC+TGG 0.523617 7_3:+52929409 None:intergenic
TTTCAATGCTTTCTTCTCTG+AGG 0.534803 7_3:+52930981 None:intergenic
AATATTCCTATTCAATATGG+TGG 0.536024 7_3:-52929472 Msa1109390:CDS
AGGCTTAATTGCATATCAGA+TGG 0.543533 7_3:+52928406 None:intergenic
AGAAGTTGAAAAGGCAAGGA+AGG 0.549154 7_3:-52928572 Msa1109390:CDS
TTTACAGTTTAAACCTGGTG+GGG 0.553174 7_3:-52930554 Msa1109390:five_prime_UTR
TAAGCCTCAAAATCAAATAG+TGG 0.553201 7_3:-52928390 Msa1109390:exon
CCTTCTGTCGTATCCGACAC+TGG 0.557554 7_3:+52930207 None:intergenic
AGGGTGTATGTTACACTCCT+CGG 0.558329 7_3:-52930074 Msa1109390:five_prime_UTR
AGGGTGTATGTTACACTCCT+CGG 0.558329 7_3:-52930242 Msa1109390:five_prime_UTR
TATTGAATAGGAATATTCTC+AGG 0.562576 7_3:+52929478 None:intergenic
TCATTCACAACAAAAGAAGC+AGG 0.565896 7_3:-52928520 Msa1109390:CDS
TGCATGGAAATGGTGAATGA+AGG 0.567134 7_3:+52931225 None:intergenic
ACTCATGGGTTTGATAGAGA+GGG 0.567192 7_3:-52930709 Msa1109390:five_prime_UTR
ATTTGGTGCTGAAAATGTTG+TGG 0.567986 7_3:-52930776 Msa1109390:five_prime_UTR
GGGTTCAAGTTCTTGAGAGA+GGG 0.569611 7_3:-52930585 Msa1109390:five_prime_UTR
TGGAAATGGTGAATGAAGGT+TGG 0.574425 7_3:+52931229 None:intergenic
GATCAAACTAACACCCCACA+TGG 0.577251 7_3:+52930909 None:intergenic
TCCATGCAGAAATTTCATCT+GGG 0.581919 7_3:-52931210 Msa1109390:five_prime_UTR
AACAACAATGGTTCCATGTG+GGG 0.585763 7_3:-52930922 Msa1109390:five_prime_UTR
AAGTGGGAGATTGTAGTTGG+AGG 0.586468 7_3:-52928652 Msa1109390:CDS
GATTAGCTTATAATAGTGAC+CGG 0.586636 7_3:-52930097 Msa1109390:five_prime_UTR
GATTAGCTTATAATAGTGAC+CGG 0.586636 7_3:-52930265 Msa1109390:five_prime_UTR
GGAGTGTAACATACACCCTC+CGG 0.592501 7_3:+52930078 None:intergenic
GGAGTGTAACATACACCCTC+CGG 0.592501 7_3:+52930246 None:intergenic
TCTGGTGAAATTAACAACAA+TGG 0.593010 7_3:-52930934 Msa1109390:five_prime_UTR
AGATAAGGAAATGTATGAGA+AGG 0.595028 7_3:-52930650 Msa1109390:five_prime_UTR
CTATAGAAGTTGAAAAGGCA+AGG 0.600332 7_3:-52928576 Msa1109390:CDS
TAACAACAATGGTTCCATGT+GGG 0.601972 7_3:-52930923 Msa1109390:five_prime_UTR
AGGGTTCAAGTTCTTGAGAG+AGG 0.610104 7_3:-52930586 Msa1109390:five_prime_UTR
CATACAAATAGAAGGAGTTG+AGG 0.610399 7_3:-52929368 Msa1109390:intron
GATCAGCCGTGTTCCAGTGT+CGG 0.611300 7_3:-52930052 Msa1109390:five_prime_UTR
GATCAGCCGTGTTCCAGTGT+CGG 0.611300 7_3:-52930220 Msa1109390:five_prime_UTR
AGCTTATAATAGTGACCGGA+GGG 0.612034 7_3:-52930093 Msa1109390:five_prime_UTR
AGCTTATAATAGTGACCGGA+GGG 0.612034 7_3:-52930261 Msa1109390:five_prime_UTR
CCATAATGATTATAACACAC+AGG 0.614159 7_3:+52930682 None:intergenic
ACCCAGATGAAATTTCTGCA+TGG 0.617097 7_3:+52931209 None:intergenic
ATACGACATGTATCCGACAC+TGG 0.622131 7_3:+52930039 None:intergenic
TACAACATGCTGAAATACCA+TGG 0.625322 7_3:+52929830 None:intergenic
AGAGTGGTGCAACAATCAAG+TGG 0.626220 7_3:-52928669 Msa1109390:CDS
GGACCATTCTGAAAATCACT+AGG 0.626931 7_3:+52929439 None:intergenic
TCTGAGTTCACTGTTAAAGG+AGG 0.633144 7_3:-52929403 Msa1109390:CDS
AAGTTTGTGATCTCTAAGGA+AGG 0.634705 7_3:-52929767 Msa1109390:CDS
ACTGGAACACGGCTGATCCG+AGG 0.635914 7_3:+52930057 None:intergenic
ACTGGAACACGGCTGATCCG+AGG 0.635914 7_3:+52930225 None:intergenic
TTACACCCAATATTGAAGCA+CGG 0.641891 7_3:-52930119 Msa1109390:five_prime_UTR
TTACACCCAATATTGAAGCA+CGG 0.641891 7_3:-52930287 Msa1109390:five_prime_UTR
ATGTATCCGACACTGGAACA+CGG 0.645239 7_3:+52930046 None:intergenic
TAGCTTATAATAGTGACCGG+AGG 0.645974 7_3:-52930094 Msa1109390:five_prime_UTR
TAGCTTATAATAGTGACCGG+AGG 0.645974 7_3:-52930262 Msa1109390:five_prime_UTR
TGGGGTGTTAGTTTGATCAA+AGG 0.650022 7_3:-52930904 Msa1109390:five_prime_UTR
GAGTGGTGCAACAATCAAGT+GGG 0.657297 7_3:-52928668 Msa1109390:CDS
CCAGTGTCGGATACGACAGA+AGG 0.658217 7_3:-52930207 Msa1109390:five_prime_UTR
CATAATGATTATAACACACA+GGG 0.662377 7_3:+52930683 None:intergenic
TTAACAACAATGGTTCCATG+TGG 0.677456 7_3:-52930924 Msa1109390:five_prime_UTR
TCGTATCCGACACTGGAACA+CGG 0.686061 7_3:+52930214 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATTACAGTGAAATAT+TGG - chr7_3:52929429-52929448 Msa1109390:CDS 15.0%
!!! TTTTTTTGTTCAGATTTATA+AGG - chr7_3:52929931-52929950 Msa1109390:intron 15.0%
!! TGTAAAATGTAGTTAAATTT+CGG + chr7_3:52930178-52930197 None:intergenic 15.0%
!!! AAATTTAACTACATTTTACA+CGG - chr7_3:52930178-52930197 Msa1109390:five_prime_UTR 15.0%
!! TGATTTAATCTTGTTAAAAA+TGG - chr7_3:52931106-52931125 Msa1109390:five_prime_UTR 15.0%
!!! TTATTTTGGTGTTGTTTATT+TGG - chr7_3:52929097-52929116 Msa1109390:intron 20.0%
!!! TTATTTTGGTGTTGTTTATT+TGG - chr7_3:52929097-52929116 Msa1109390:intron 20.0%
!! TTTCATGTCATACTAAATAT+AGG + chr7_3:52929546-52929565 None:intergenic 20.0%
!!! AGATGTAATTTTAGAAAATC+TGG - chr7_3:52929748-52929767 Msa1109390:CDS 20.0%
!! ACAATAATAGAAAAATTGTG+GGG + chr7_3:52929888-52929907 None:intergenic 20.0%
!! CACAATAATAGAAAAATTGT+GGG + chr7_3:52929889-52929908 None:intergenic 20.0%
!! TCTTATTAGAGACTATATAA+TGG - chr7_3:52930112-52930131 Msa1109390:five_prime_UTR 20.0%
!! AAGTATGAGTATATCAAAAT+TGG + chr7_3:52930466-52930485 None:intergenic 20.0%
!!! AATTTTGATATACTCATACT+TGG - chr7_3:52930465-52930484 Msa1109390:five_prime_UTR 20.0%
!!! ATTTTGATATACTCATACTT+GGG - chr7_3:52930466-52930485 Msa1109390:five_prime_UTR 20.0%
!!! CAATATTATTATGTTTTTGC+TGG - chr7_3:52931071-52931090 Msa1109390:five_prime_UTR 20.0%
! CATAATGATTATAACACACA+GGG + chr7_3:52928752-52928771 None:intergenic 25.0%
! ACAAAAACAATGTCATATCA+AGG + chr7_3:52929026-52929045 None:intergenic 25.0%
!!! TTGTTGCTTTTATTTGAAGA+AGG - chr7_3:52929057-52929076 Msa1109390:intron 25.0%
!!! CGTGTATTGTTTTGTTATTT+TGG - chr7_3:52929083-52929102 Msa1109390:intron 25.0%
!!! CGTGTATTGTTTTGTTATTT+TGG - chr7_3:52929083-52929102 Msa1109390:intron 25.0%
!! GGATTTTCTCAAATTGTTAA+CGG - chr7_3:52929450-52929469 Msa1109390:CDS 25.0%
!! CATAAACATCATTGATGTTT+TGG + chr7_3:52929721-52929740 None:intergenic 25.0%
!!! ATTTTGTTTGATACATAGAC+AGG + chr7_3:52929855-52929874 None:intergenic 25.0%
! GCACAATAATAGAAAAATTG+TGG + chr7_3:52929890-52929909 None:intergenic 25.0%
! TATTGAATAGGAATATTCTC+AGG + chr7_3:52929957-52929976 None:intergenic 25.0%
! GAGAATATTCCTATTCAATA+TGG - chr7_3:52929957-52929976 Msa1109390:intron 25.0%
! AATATTCCTATTCAATATGG+TGG - chr7_3:52929960-52929979 Msa1109390:intron 25.0%
! AAAGTGAACATACAAATAGA+AGG - chr7_3:52930056-52930075 Msa1109390:five_prime_UTR 25.0%
! GACTATATAATGGTTACTTA+TGG - chr7_3:52930122-52930141 Msa1109390:five_prime_UTR 25.0%
! TAATGGTTACTTATGGTTTA+AGG - chr7_3:52930129-52930148 Msa1109390:five_prime_UTR 25.0%
! GTAGTTAACGAAATGAAATT+CGG + chr7_3:52930271-52930290 None:intergenic 25.0%
!!! TGTGATTAGTCACAATTTTT+AGG - chr7_3:52930300-52930319 Msa1109390:five_prime_UTR 25.0%
!! TTTTGATATACTCATACTTG+GGG - chr7_3:52930467-52930486 Msa1109390:five_prime_UTR 25.0%
! AAATCTTATACTAAGTGTCA+CGG + chr7_3:52930526-52930545 None:intergenic 25.0%
!!! ACGTTTGATTTTTGTGTTAA+TGG - chr7_3:52930629-52930648 Msa1109390:five_prime_UTR 25.0%
! GTAGTAAATTGCTTTAACAT+TGG + chr7_3:52930699-52930718 None:intergenic 25.0%
!!! GTGTATTGTTGTTGTATTTT+CGG + chr7_3:52931007-52931026 None:intergenic 25.0%
! TTGTTAAAAATGGTACTTAG+TGG - chr7_3:52931116-52931135 Msa1109390:five_prime_UTR 25.0%
!!! ATGAAGGTTGGTTTTGAAAT+GGG + chr7_3:52928194-52928213 None:intergenic 30.0%
!!! AATGAAGGTTGGTTTTGAAA+TGG + chr7_3:52928195-52928214 None:intergenic 30.0%
ATGAAATTTCTGCATGGAAA+TGG + chr7_3:52928220-52928239 None:intergenic 30.0%
! TTTCTTGGAGTTTCTAGAAA+TGG + chr7_3:52928321-52928340 None:intergenic 30.0%
!!! GTTCTTTTGTTTGGTTTTGA+GGG + chr7_3:52928348-52928367 None:intergenic 30.0%
AACCATAGTCACAACATTAA+TGG - chr7_3:52928365-52928384 Msa1109390:three_prime_UTR 30.0%
TTCCATTAATGTTGTGACTA+TGG + chr7_3:52928370-52928389 None:intergenic 30.0%
! TTTGAGGTTGGAAATGAAAT+AGG + chr7_3:52928430-52928449 None:intergenic 30.0%
TCTGGTGAAATTAACAACAA+TGG - chr7_3:52928498-52928517 Msa1109390:CDS 30.0%
AAATGTTGTGGATGAAGATT+TGG - chr7_3:52928668-52928687 Msa1109390:CDS 30.0%
AATGTTGTGGATGAAGATTT+GGG - chr7_3:52928669-52928688 Msa1109390:CDS 30.0%
CCATAATGATTATAACACAC+AGG + chr7_3:52928753-52928772 None:intergenic 30.0%
CCTGTGTGTTATAATCATTA+TGG - chr7_3:52928750-52928769 Msa1109390:intron 30.0%
TTATGGTGTGTTCAAAGATA+AGG - chr7_3:52928767-52928786 Msa1109390:intron 30.0%
AGATAAGGAAATGTATGAGA+AGG - chr7_3:52928782-52928801 Msa1109390:intron 30.0%
! GAAATGTATGAGAAGGTTTT+TGG - chr7_3:52928789-52928808 Msa1109390:intron 30.0%
!! AGTTGAAGAAGTTTTTGAGA+TGG - chr7_3:52928823-52928842 Msa1109390:intron 30.0%
! AAGCTTTTACAGTTTAAACC+TGG - chr7_3:52928873-52928892 Msa1109390:intron 30.0%
! TTTTACAGTTTAAACCTGGT+GGG - chr7_3:52928877-52928896 Msa1109390:intron 30.0%
! GATTCAAGTTACTGATCTTA+AGG - chr7_3:52928911-52928930 Msa1109390:intron 30.0%
TCAAGATAACTATCCTGAAA+TGG - chr7_3:52928986-52929005 Msa1109390:intron 30.0%
!! GATTAGCTTATAATAGTGAC+CGG - chr7_3:52929167-52929186 Msa1109390:intron 30.0%
!! GATTAGCTTATAATAGTGAC+CGG - chr7_3:52929167-52929186 Msa1109390:intron 30.0%
GTAATTATATGTGTGAGTGT+CGG + chr7_3:52929419-52929438 None:intergenic 30.0%
! TTAGTTCTTTGAGTCAGAAA+TGG + chr7_3:52929641-52929660 None:intergenic 30.0%
! TGTGATTGTTTCCTTTTTCT+AGG - chr7_3:52929808-52929827 Msa1109390:CDS 30.0%
CAAAAGTAAAGCCTAGAAAA+AGG + chr7_3:52929822-52929841 None:intergenic 30.0%
! ACAGTTGAAACATCTGAAAT+CGG - chr7_3:52930338-52930357 Msa1109390:five_prime_UTR 30.0%
GTTTGTAACGTTCAAATTTG+TGG - chr7_3:52930362-52930381 Msa1109390:five_prime_UTR 30.0%
ACACTTAGTATAAGATTTCC+AGG - chr7_3:52930528-52930547 Msa1109390:five_prime_UTR 30.0%
!! TCACAATTTGTTGATTAGAG+TGG - chr7_3:52930747-52930766 Msa1109390:five_prime_UTR 30.0%
CTACACTATAGAAGTTGAAA+AGG - chr7_3:52930851-52930870 Msa1109390:five_prime_UTR 30.0%
TAAGCCTCAAAATCAAATAG+TGG - chr7_3:52931042-52931061 Msa1109390:five_prime_UTR 30.0%
! ACTTGTTTGGTTTATGATGT+AGG - chr7_3:52931148-52931167 Msa1109390:five_prime_UTR 30.0%
!! ATTGTGATGAGTTTTATCAG+AGG - chr7_3:52931237-52931256 Msa1109390:five_prime_UTR 30.0%
!! TTGTGATGAGTTTTATCAGA+GGG - chr7_3:52931238-52931257 Msa1109390:five_prime_UTR 30.0%
AATCGAGTGATTATAGTCAT+TGG - chr7_3:52931301-52931320 Msa1109390:five_prime_UTR 30.0%
!!! TGAAGGTTGGTTTTGAAATG+GGG + chr7_3:52928193-52928212 None:intergenic 35.0%
TTCCATGCAGAAATTTCATC+TGG - chr7_3:52928221-52928240 Msa1109390:three_prime_UTR 35.0%
TCCATGCAGAAATTTCATCT+GGG - chr7_3:52928222-52928241 Msa1109390:three_prime_UTR 35.0%
AATTCCTCATTCACCATCAA+TGG - chr7_3:52928254-52928273 Msa1109390:three_prime_UTR 35.0%
! TTTCTAGAAATGGTGTGCTT+AGG + chr7_3:52928311-52928330 None:intergenic 35.0%
!!! GGTTCTTTTGTTTGGTTTTG+AGG + chr7_3:52928349-52928368 None:intergenic 35.0%
! GTGACTATGGTTCTTTTGTT+TGG + chr7_3:52928357-52928376 None:intergenic 35.0%
TTTCAATGCTTTCTTCTCTG+AGG + chr7_3:52928454-52928473 None:intergenic 35.0%
!! GAAAGCATTGAAACAGTTGA+AGG - chr7_3:52928461-52928480 Msa1109390:CDS 35.0%
AAGGAAAAGCTCTTAACTTC+TGG - chr7_3:52928480-52928499 Msa1109390:CDS 35.0%
TTAACAACAATGGTTCCATG+TGG - chr7_3:52928508-52928527 Msa1109390:CDS 35.0%
TAACAACAATGGTTCCATGT+GGG - chr7_3:52928509-52928528 Msa1109390:CDS 35.0%
CTTTCTTGGAGGAAAGAATT+TGG - chr7_3:52928639-52928658 Msa1109390:CDS 35.0%
!! ATTTGGTGCTGAAAATGTTG+TGG - chr7_3:52928656-52928675 Msa1109390:CDS 35.0%
!!! GTGTTGTTGCTTTTACTCAT+GGG - chr7_3:52928709-52928728 Msa1109390:intron 35.0%
!!! TGAAGAAGTTTTTGAGATGG+AGG - chr7_3:52928826-52928845 Msa1109390:intron 35.0%
!!! GAAGAAGTTTTTGAGATGGA+GGG - chr7_3:52928827-52928846 Msa1109390:intron 35.0%
! CTTTTACAGTTTAAACCTGG+TGG - chr7_3:52928876-52928895 Msa1109390:intron 35.0%
TTTACAGTTTAAACCTGGTG+GGG - chr7_3:52928878-52928897 Msa1109390:intron 35.0%
ATATGCCTAAGAGTGAGTTA+AGG - chr7_3:52928934-52928953 Msa1109390:intron 35.0%
TATGCCTAAGAGTGAGTTAA+GGG - chr7_3:52928935-52928954 Msa1109390:intron 35.0%
TTACACCCAATATTGAAGCA+CGG - chr7_3:52929145-52929164 Msa1109390:intron 35.0%
CTAATCCGTGCTTCAATATT+GGG + chr7_3:52929153-52929172 None:intergenic 35.0%
TTACACCCAATATTGAAGCA+CGG - chr7_3:52929145-52929164 Msa1109390:intron 35.0%
CTAATCCGTGCTTCAATATT+GGG + chr7_3:52929153-52929172 None:intergenic 35.0%
TCTCAAATTGTTAACGGTGA+TGG - chr7_3:52929456-52929475 Msa1109390:CDS 35.0%
! AGATTTTCATCAATGTGCCA+TGG - chr7_3:52929585-52929604 Msa1109390:intron 35.0%
TACAACATGCTGAAATACCA+TGG + chr7_3:52929605-52929624 None:intergenic 35.0%
! AAGTTTGTGATCTCTAAGGA+AGG - chr7_3:52929665-52929684 Msa1109390:intron 35.0%
CTCAATCCACCATATTGAAT+AGG + chr7_3:52929969-52929988 None:intergenic 35.0%
CATACAAATAGAAGGAGTTG+AGG - chr7_3:52930064-52930083 Msa1109390:five_prime_UTR 35.0%
AACTCGCAAACAAATATGCT+CGG - chr7_3:52930558-52930577 Msa1109390:five_prime_UTR 35.0%
CTATAGAAGTTGAAAAGGCA+AGG - chr7_3:52930856-52930875 Msa1109390:five_prime_UTR 35.0%
TCATTCACAACAAAAGAAGC+AGG - chr7_3:52930912-52930931 Msa1109390:five_prime_UTR 35.0%
AACAAAAGAAGCAGGCATAA+TGG - chr7_3:52930920-52930939 Msa1109390:five_prime_UTR 35.0%
AGGCTTAATTGCATATCAGA+TGG + chr7_3:52931029-52931048 None:intergenic 35.0%
!! GTGACCACTATTTGATTTTG+AGG + chr7_3:52931049-52931068 None:intergenic 35.0%
GTGGTGTATTAGTACTTGTT+TGG - chr7_3:52931135-52931154 Msa1109390:five_prime_UTR 35.0%
!! TGTGATGAGTTTTATCAGAG+GGG - chr7_3:52931239-52931258 Msa1109390:five_prime_UTR 35.0%
TGGAAATGGTGAATGAAGGT+TGG + chr7_3:52928206-52928225 None:intergenic 40.0%
TGCATGGAAATGGTGAATGA+AGG + chr7_3:52928210-52928229 None:intergenic 40.0%
ACCCAGATGAAATTTCTGCA+TGG + chr7_3:52928226-52928245 None:intergenic 40.0%
!! TCTTCCATTGATGGTGAATG+AGG + chr7_3:52928261-52928280 None:intergenic 40.0%
GAGTGATCTTCTTCCATTGA+TGG + chr7_3:52928270-52928289 None:intergenic 40.0%
!!! GGTTTTGAGGGAGTTTTTCT+TGG + chr7_3:52928336-52928355 None:intergenic 40.0%
! CTTTCTTCTCTGAGGATTTG+AGG + chr7_3:52928446-52928465 None:intergenic 40.0%
AACAACAATGGTTCCATGTG+GGG - chr7_3:52928510-52928529 Msa1109390:CDS 40.0%
! TGGGGTGTTAGTTTGATCAA+AGG - chr7_3:52928528-52928547 Msa1109390:CDS 40.0%
! TGCTTGTGAAGTGTCTTTCT+TGG - chr7_3:52928625-52928644 Msa1109390:CDS 40.0%
! TTGTGAAGTGTCTTTCTTGG+AGG - chr7_3:52928628-52928647 Msa1109390:CDS 40.0%
TTGGGATTCAAAGAGCTTGA+AGG - chr7_3:52928687-52928706 Msa1109390:intron 40.0%
!!! GGTGTTGTTGCTTTTACTCA+TGG - chr7_3:52928708-52928727 Msa1109390:intron 40.0%
TACTCATGGGTTTGATAGAG+AGG - chr7_3:52928722-52928741 Msa1109390:intron 40.0%
ACTCATGGGTTTGATAGAGA+GGG - chr7_3:52928723-52928742 Msa1109390:intron 40.0%
ACAACCCTTAACTCACTCTT+AGG + chr7_3:52928942-52928961 None:intergenic 40.0%
GCTAATCCGTGCTTCAATAT+TGG + chr7_3:52929154-52929173 None:intergenic 40.0%
TAGCTTATAATAGTGACCGG+AGG - chr7_3:52929170-52929189 Msa1109390:intron 40.0%
AGCTTATAATAGTGACCGGA+GGG - chr7_3:52929171-52929190 Msa1109390:intron 40.0%
GCTAATCCGTGCTTCAATAT+TGG + chr7_3:52929154-52929173 None:intergenic 40.0%
TAGCTTATAATAGTGACCGG+AGG - chr7_3:52929170-52929189 Msa1109390:intron 40.0%
AGCTTATAATAGTGACCGGA+GGG - chr7_3:52929171-52929190 Msa1109390:intron 40.0%
GAGCAAGTTTGTGATCTCTA+AGG - chr7_3:52929661-52929680 Msa1109390:intron 40.0%
! CGACCTAGTGATTTTCAGAA+TGG - chr7_3:52929990-52930009 Msa1109390:intron 40.0%
! GGACCATTCTGAAAATCACT+AGG + chr7_3:52929996-52930015 None:intergenic 40.0%
!!! TGAACTCAGAAGCTGGTTTT+GGG + chr7_3:52930019-52930038 None:intergenic 40.0%
TTAACAGTGAACTCAGAAGC+TGG + chr7_3:52930026-52930045 None:intergenic 40.0%
GCTTCTGAGTTCACTGTTAA+AGG - chr7_3:52930026-52930045 Msa1109390:intron 40.0%
TCTGAGTTCACTGTTAAAGG+AGG - chr7_3:52930029-52930048 Msa1109390:intron 40.0%
!!! AGGTTCTGAGTTCGAACTTT+TGG - chr7_3:52930149-52930168 Msa1109390:five_prime_UTR 40.0%
ACGAAATGAAATTCGGTAGC+TGG + chr7_3:52930264-52930283 None:intergenic 40.0%
TAAAGTTGACTACCTAGCGA+CGG + chr7_3:52930502-52930521 None:intergenic 40.0%
ATCAAGTGGGAGATTGTAGT+TGG - chr7_3:52930777-52930796 Msa1109390:five_prime_UTR 40.0%
AGAAGTTGAAAAGGCAAGGA+AGG - chr7_3:52930860-52930879 Msa1109390:five_prime_UTR 40.0%
AGCAGGCATAATGGTACTTT+CGG - chr7_3:52930929-52930948 Msa1109390:five_prime_UTR 40.0%
! CTTCTCTGAGGATTTGAGGT+TGG + chr7_3:52928442-52928461 None:intergenic 45.0%
GATCAAACTAACACCCCACA+TGG + chr7_3:52928526-52928545 None:intergenic 45.0%
! AGGGTTCAAGTTCTTGAGAG+AGG - chr7_3:52928846-52928865 Msa1109390:intron 45.0%
! GGGTTCAAGTTCTTGAGAGA+GGG - chr7_3:52928847-52928866 Msa1109390:intron 45.0%
ATCAAAGAATTCACCCCACC+AGG + chr7_3:52928894-52928913 None:intergenic 45.0%
TCCTGAAATGGTTGCTCGTA+AGG - chr7_3:52928998-52929017 Msa1109390:intron 45.0%
ACCTTACGAGCAACCATTTC+AGG + chr7_3:52929002-52929021 None:intergenic 45.0%
AGGGTGTATGTTACACTCCT+CGG - chr7_3:52929190-52929209 Msa1109390:intron 45.0%
AGGGTGTATGTTACACTCCT+CGG - chr7_3:52929190-52929209 Msa1109390:intron 45.0%
ATGTATCCGACACTGGAACA+CGG + chr7_3:52929389-52929408 None:intergenic 45.0%
ATACGACATGTATCCGACAC+TGG + chr7_3:52929396-52929415 None:intergenic 45.0%
GGTGTGTTAGTATCCGTGTT+CGG - chr7_3:52929477-52929496 Msa1109390:CDS 45.0%
AGTCCTATCAAGCACGGATA+CGG + chr7_3:52929519-52929538 None:intergenic 45.0%
GGTGTAAGTCCTATCAAGCA+CGG + chr7_3:52929525-52929544 None:intergenic 45.0%
!!! AACTCAGAAGCTGGTTTTGG+GGG + chr7_3:52930017-52930036 None:intergenic 45.0%
!!! GAACTCAGAAGCTGGTTTTG+GGG + chr7_3:52930018-52930037 None:intergenic 45.0%
!! GTGAACTCAGAAGCTGGTTT+TGG + chr7_3:52930020-52930039 None:intergenic 45.0%
GTTTGCGAGTTTCTTGATCC+TGG + chr7_3:52930549-52930568 None:intergenic 45.0%
AGAGTGGTGCAACAATCAAG+TGG - chr7_3:52930763-52930782 Msa1109390:five_prime_UTR 45.0%
GAGTGGTGCAACAATCAAGT+GGG - chr7_3:52930764-52930783 Msa1109390:five_prime_UTR 45.0%
AAGTGGGAGATTGTAGTTGG+AGG - chr7_3:52930780-52930799 Msa1109390:five_prime_UTR 45.0%
! GTGTAGCTAGCTTCTGCATT+TGG + chr7_3:52930837-52930856 None:intergenic 45.0%
GGAGTGTAACATACACCCTC+CGG + chr7_3:52929189-52929208 None:intergenic 50.0%
TCGTATCCGACACTGGAACA+CGG + chr7_3:52929221-52929240 None:intergenic 50.0%
GGAGTGTAACATACACCCTC+CGG + chr7_3:52929189-52929208 None:intergenic 50.0%
!! TAGTATCCGTGTTCGGTGTC+CGG - chr7_3:52929484-52929503 Msa1109390:CDS 50.0%
ATCAAGCACGGATACGGACA+CGG + chr7_3:52929513-52929532 None:intergenic 50.0%
TGTCCGTATCCGTGCTTGAT+AGG - chr7_3:52929513-52929532 Msa1109390:intron 50.0%
CGCAAACAAATATGCTCGGC+TGG - chr7_3:52930562-52930581 Msa1109390:five_prime_UTR 50.0%
GGAGATTGTAGTTGGAGGCT+GGG - chr7_3:52930785-52930804 Msa1109390:five_prime_UTR 50.0%
TGTAGTTGGAGGCTGGGATT+TGG - chr7_3:52930791-52930810 Msa1109390:five_prime_UTR 50.0%
CGGTCGATAACTCTGCTTCT+AGG - chr7_3:52930949-52930968 Msa1109390:five_prime_UTR 50.0%
GATCAGCCGTGTTCCAGTGT+CGG - chr7_3:52929212-52929231 Msa1109390:intron 55.0%
CCTTCTGTCGTATCCGACAC+TGG + chr7_3:52929228-52929247 None:intergenic 55.0%
! CCAGTGTCGGATACGACAGA+AGG - chr7_3:52929225-52929244 Msa1109390:intron 55.0%
GATCAGCCGTGTTCCAGTGT+CGG - chr7_3:52929212-52929231 Msa1109390:intron 55.0%
TTGACTACCTAGCGACGGAG+TGG + chr7_3:52930497-52930516 None:intergenic 55.0%
GGGAGATTGTAGTTGGAGGC+TGG - chr7_3:52930784-52930803 Msa1109390:five_prime_UTR 55.0%
ACTGGAACACGGCTGATCCG+AGG + chr7_3:52929210-52929229 None:intergenic 60.0%
ACTGGAACACGGCTGATCCG+AGG + chr7_3:52929210-52929229 None:intergenic 60.0%
ACGGATACGGACACGGACAC+CGG + chr7_3:52929506-52929525 None:intergenic 60.0%
CGGACACCGGACACCGAACA+CGG + chr7_3:52929493-52929512 None:intergenic 65.0%
GGGTCATCCACTCCGTCGCT+AGG - chr7_3:52930487-52930506 Msa1109390:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 52928083 52931371 52928083 ID=Msa1109390;Name=Msa1109390
chr7_3 mRNA 52928083 52931371 52928083 ID=Msa1109390-mRNA-1;Parent=Msa1109390;Name=Msa1109390-mRNA-1;_AED=0.02;_eAED=0.02;_QI=1359|1|1|1|0.66|0.75|4|310|177
chr7_3 exon 52928083 52928689 52928083 ID=Msa1109390-mRNA-1:exon:11153;Parent=Msa1109390-mRNA-1
chr7_3 exon 52929369 52929510 52929369 ID=Msa1109390-mRNA-1:exon:11152;Parent=Msa1109390-mRNA-1
chr7_3 exon 52929743 52929867 52929743 ID=Msa1109390-mRNA-1:exon:11151;Parent=Msa1109390-mRNA-1
chr7_3 exon 52930043 52931371 52930043 ID=Msa1109390-mRNA-1:exon:11150;Parent=Msa1109390-mRNA-1
chr7_3 five_prime_UTR 52930043 52931371 52930043 ID=Msa1109390-mRNA-1:five_prime_utr;Parent=Msa1109390-mRNA-1
chr7_3 five_prime_UTR 52929838 52929867 52929838 ID=Msa1109390-mRNA-1:five_prime_utr;Parent=Msa1109390-mRNA-1
chr7_3 CDS 52929743 52929837 52929743 ID=Msa1109390-mRNA-1:cds;Parent=Msa1109390-mRNA-1
chr7_3 CDS 52929369 52929510 52929369 ID=Msa1109390-mRNA-1:cds;Parent=Msa1109390-mRNA-1
chr7_3 CDS 52928393 52928689 52928393 ID=Msa1109390-mRNA-1:cds;Parent=Msa1109390-mRNA-1
chr7_3 three_prime_UTR 52928083 52928392 52928083 ID=Msa1109390-mRNA-1:three_prime_utr;Parent=Msa1109390-mRNA-1
Gene Sequence

>Msa1109390

ATGTTGTATTCAATGTTCAGTCCATTTCTGACTCAAAGAACTAAGAGCAAGTTTGTGATCTCTAAGGAAGGAAATGCTGCAGAAACACTCTACAAATTTATAAGGCCTGAGAATATTCCTATTCAATATGGTGGATTGAGTCGACCTAGTGATTTTCAGAATGGTCCCCCAAAACCAGCTTCTGAGTTCACTGTTAAAGGAGGAGAAAAAGTGAACATACAAATAGAAGGAGTTGAGAGTGGTGCAACAATCAAGTGGGAGATTGTAGTTGGAGGCTGGGATTTGGAATACAGTGCTGAGTTTGTTCCAAATGCAGAAGCTAGCTACACTATAGAAGTTGAAAAGGCAAGGAAGGTTAATGCATCAGAAGAAGCAATTCAGAATTCATTCACAACAAAAGAAGCAGGCATAATGGTACTTTCGGTCGATAACTCTGCTTCTAGGAAGAAAAAAGTCGCTGCTTATCGCTATATTGTCCGAAAATACAACAACAATACACCATCTGATATGCAATTAAGCCTCAAAATCAAATAG
Protein sequence

>Msa1109390

MLYSMFSPFLTQRTKSKFVISKEGNAAETLYKFIRPENIPIQYGGLSRPSDFQNGPPKPASEFTVKGGEKVNIQIEGVESGATIKWEIVVGGWDLEYSAEFVPNAEASYTIEVEKARKVNASEEAIQNSFTTKEAGIMVLSVDNSASRKKKVAAYRYIVRKYNNNTPSDMQLSLKIK*