AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1244660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1244660 MtrunA17_Chr8g0360371 88.961 308 29 3 1 308 1 303 2.09e-149 421
Msa1244660 MtrunA17_Chr2g0317231 68.033 122 38 1 1 122 3 123 1.44e-62 213
Msa1244660 MtrunA17_Chr2g0324711 41.724 290 146 8 7 276 8 294 1.88e-54 181
Msa1244660 MtrunA17_Chr2g0324721 46.927 179 86 3 1 173 4 179 8.41e-48 159
Msa1244660 MtrunA17_Chr8g0385031 40.000 250 126 6 1 232 1 244 2.20e-44 153
Msa1244660 MtrunA17_Chr8g0385021 39.130 253 130 5 1 232 1 250 4.98e-43 149
Msa1244660 MtrunA17_Chr4g0040291 51.887 106 51 0 20 125 21 126 3.03e-39 136
Msa1244660 MtrunA17_Chr8g0385041 40.183 219 102 7 22 231 23 221 6.08e-39 139
Msa1244660 MtrunA17_Chr4g0041811 46.281 121 63 2 7 125 15 135 7.63e-36 128
Msa1244660 MtrunA17_Chr3g0136871 45.833 144 66 4 21 161 24 158 1.49e-35 126
Msa1244660 MtrunA17_Chr1g0150131 45.455 121 63 2 8 125 9 129 9.22e-35 124
Msa1244660 MtrunA17_Chr3g0127521 36.797 231 121 4 1 206 2 232 8.04e-33 122
Msa1244660 MtrunA17_Chr2g0331181 48.000 125 57 3 12 133 18 137 1.04e-31 117
Msa1244660 MtrunA17_Chr2g0319611 43.478 115 61 3 21 131 23 137 1.79e-30 115
Msa1244660 MtrunA17_Chr6g0458331 49.541 109 50 2 26 131 39 145 1.76e-29 110
Msa1244660 MtrunA17_Chr4g0039721 38.356 146 75 5 5 142 12 150 1.94e-27 105
Msa1244660 MtrunA17_Chr4g0062341 36.970 165 86 5 9 163 12 168 1.13e-26 103
Msa1244660 MtrunA17_Chr4g0062311 36.970 165 86 5 9 163 12 168 1.13e-26 103
Msa1244660 MtrunA17_Chr7g0256441 38.889 144 59 5 21 161 24 141 5.94e-24 95.1
Msa1244660 MtrunA17_Chr5g0394321 31.410 156 105 1 7 162 9 162 1.65e-22 92.4
Msa1244660 MtrunA17_Chr4g0073901 34.167 120 74 4 7 121 10 129 7.87e-20 85.1
Msa1244660 MtrunA17_Chr2g0323021 32.031 128 83 3 4 130 5 129 3.19e-18 82.0
Msa1244660 MtrunA17_Chr2g0289741 34.959 123 71 4 11 132 15 129 1.06e-17 79.0
Msa1244660 MtrunA17_Chr8g0379081 49.275 69 33 1 69 135 2 70 4.10e-17 76.3
Msa1244660 MtrunA17_Chr4g0073921 34.146 123 77 4 5 123 9 131 5.74e-17 79.3
Msa1244660 MtrunA17_Chr4g0064041 37.000 100 61 1 24 121 191 290 7.18e-17 80.1
Msa1244660 MtrunA17_Chr4g0064041 31.111 135 84 2 10 135 8 142 2.32e-16 78.6
Msa1244660 MtrunA17_Chr2g0306461 35.043 117 73 3 7 121 10 125 1.34e-16 76.6
Msa1244660 MtrunA17_Chr5g0440711 34.307 137 83 5 4 137 9 141 3.24e-16 74.3
Msa1244660 MtrunA17_Chr1g0188981 31.731 104 68 1 21 121 26 129 8.62e-16 74.3
Msa1244660 MtrunA17_Chr1g0205701 31.250 112 77 0 11 122 12 123 1.40e-15 73.6
Msa1244660 MtrunA17_Chr8g0387804 32.258 124 78 2 4 121 4 127 1.89e-15 71.6
Msa1244660 MtrunA17_Chr3g0132431 34.752 141 85 4 1 138 1 137 3.72e-15 72.4
Msa1244660 MtrunA17_Chr4g0064031 36.290 124 73 4 16 134 20 142 8.85e-15 70.9
Msa1244660 MtrunA17_Chr4g0033131 32.117 137 80 5 7 137 10 139 1.17e-14 70.9
Msa1244660 MtrunA17_Chr4g0033531 32.117 137 80 5 7 137 10 139 1.17e-14 70.9
Msa1244660 MtrunA17_Chr1g0196801 33.793 145 86 4 18 155 17 158 1.58e-14 70.5
Msa1244660 MtrunA17_Chr6g0453921 36.232 138 79 5 1 135 17 148 2.84e-14 69.7
Msa1244660 MtrunA17_Chr8g0335531 36.478 159 83 8 5 153 6 156 4.17e-14 69.7
Msa1244660 MtrunA17_Chr1g0211101 27.632 152 101 4 3 150 12 158 8.89e-14 68.6
Msa1244660 MtrunA17_Chr3g0132441 37.097 124 71 4 1 121 1 120 5.13e-13 66.6
Msa1244660 MtrunA17_Chr8g0335571 35.833 120 71 4 5 122 6 121 5.66e-13 66.6
Msa1244660 MtrunA17_Chr6g0453801 36.667 120 70 4 5 121 15 131 7.01e-13 65.9
Msa1244660 MtrunA17_Chr7g0253641 31.667 120 76 3 5 122 7 122 6.67e-12 64.3
Msa1244660 MtrunA17_Chr6g0481491 31.933 119 74 3 3 121 11 122 8.75e-12 63.5
Msa1244660 MtrunA17_Chr3g0132511 35.000 100 62 2 23 121 25 122 2.27e-11 62.4
Msa1244660 MtrunA17_Chr6g0483981 33.140 172 91 6 7 172 8 161 4.82e-11 63.5
Msa1244660 MtrunA17_Chr6g0459441 34.021 97 60 3 26 121 28 121 5.05e-11 60.8
Msa1244660 MtrunA17_Chr3g0132411 32.743 113 72 3 5 117 47 155 8.05e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1244660 AT4G27520.1 54.198 131 56 1 1 127 4 134 1.11e-49 169
Msa1244660 AT5G53870.2 47.917 192 82 5 8 197 12 187 5.37e-49 167
Msa1244660 AT5G53870.1 47.917 192 82 5 8 197 12 187 5.95e-49 167
Msa1244660 AT4G28365.1 48.366 153 72 3 1 146 5 157 2.29e-47 158
Msa1244660 AT4G32490.1 50.926 108 53 0 16 123 23 130 4.10e-41 142
Msa1244660 AT2G25060.1 43.678 174 76 5 4 174 13 167 1.63e-38 134
Msa1244660 AT4G31840.1 46.259 147 75 3 5 148 8 153 1.27e-36 129
Msa1244660 AT5G57920.2 41.667 120 67 2 8 124 6 125 2.28e-32 119
Msa1244660 AT5G57920.1 44.954 109 57 2 19 124 18 126 2.55e-32 118
Msa1244660 AT5G57920.3 42.188 128 69 3 19 141 50 177 8.10e-32 118
Msa1244660 AT4G30590.1 38.922 167 79 4 1 160 1 151 9.43e-32 117
Msa1244660 AT5G25090.1 43.802 121 65 2 8 125 10 130 1.60e-31 116
Msa1244660 AT3G20570.1 37.143 175 98 3 9 182 15 178 1.80e-30 114
Msa1244660 AT1G48940.1 45.902 122 56 3 5 122 11 126 2.61e-27 105
Msa1244660 AT2G23990.1 39.744 156 79 5 5 156 8 152 1.05e-26 104
Msa1244660 AT1G79800.1 39.073 151 74 5 4 146 11 151 5.40e-26 102
Msa1244660 AT5G14345.1 44.545 110 55 3 28 134 20 126 2.59e-25 99.0
Msa1244660 AT3G18590.1 45.082 122 60 3 5 122 9 127 3.73e-25 100
Msa1244660 AT2G23990.2 36.000 175 78 6 5 156 8 171 3.06e-22 93.2
Msa1244660 AT2G31050.1 39.450 109 65 1 26 134 31 138 4.47e-20 86.7
Msa1244660 AT5G26330.1 34.513 113 74 0 12 124 12 124 3.84e-18 81.3
Msa1244660 AT2G26720.1 37.113 97 60 1 26 122 31 126 6.44e-17 78.2
Msa1244660 AT5G07475.1 31.507 146 93 4 1 143 7 148 8.58e-17 77.4
Msa1244660 AT2G32300.1 30.128 156 92 3 10 165 12 150 3.28e-16 77.4
Msa1244660 AT2G32300.2 30.128 156 92 3 10 165 42 180 4.66e-15 74.7
Msa1244660 AT3G17675.1 30.252 119 79 2 5 121 11 127 6.89e-15 70.5
Msa1244660 AT3G60270.1 36.893 103 60 3 21 122 23 121 3.40e-14 70.1
Msa1244660 AT5G15350.1 35.000 120 73 3 23 140 28 144 9.52e-14 68.6
Msa1244660 AT1G64640.1 31.933 119 79 2 24 141 33 150 3.39e-13 67.4
Msa1244660 AT4G12880.1 34.653 101 61 3 23 121 28 125 4.57e-12 63.2
Msa1244660 AT2G27035.1 30.070 143 84 5 10 151 15 142 6.91e-12 63.2
Msa1244660 AT1G72230.1 33.613 119 68 3 3 121 10 117 1.09e-11 63.2
Msa1244660 AT5G20230.1 32.353 102 66 2 23 122 24 124 1.12e-11 63.2
Msa1244660 AT3G27200.1 29.600 125 82 4 1 122 1 122 2.12e-11 62.0

Find 135 sgRNAs with CRISPR-Local

Find 352 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAGCTCCTTCGAGCTTTC+TGG 0.120831 8_2:-33602667 Msa1244660:CDS
CTTGGTGAAGGAGATGATTT+TGG 0.279381 8_2:+33603143 None:intergenic
CCATCTTCGAATTTCTTGAT+TGG 0.318575 8_2:+33603281 None:intergenic
GGTCCTCCGGCTCCAGGACC+CGG 0.322983 8_2:-33602816 Msa1244660:CDS
TACCTATAGTGTCACTGATT+TGG 0.323312 8_2:+33603889 None:intergenic
GCCGGAACAGGGGAGCTTGC+TGG 0.329366 8_2:+33603032 None:intergenic
CCCCGCTGGAGCGGCTCCCC+CGG 0.330921 8_2:+33602737 None:intergenic
CATCTTCGAATTTCTTGATT+GGG 0.341423 8_2:+33603282 None:intergenic
CCACCAGGGGCACCAGGACC+CGG 0.348851 8_2:-33602780 Msa1244660:CDS
GGACCACCTGCTGGGGTAGA+TGG 0.356874 8_2:+33602834 None:intergenic
GGAGCCGGAGGACCACCTGC+TGG 0.368310 8_2:+33602825 None:intergenic
TGGTGGAGTCGCTGCTCCCC+CGG 0.370647 8_2:+33602797 None:intergenic
TCCAGCAAGCTCCCCTGTTC+CGG 0.374050 8_2:-33603033 Msa1244660:CDS
GTAGGTCCACCAGCCGGAAC+AGG 0.383142 8_2:+33603020 None:intergenic
TCCTGGTGTCCCTGATCCCC+CGG 0.386729 8_2:+33602761 None:intergenic
ATCAGGGACACCAGGACCCG+GGG 0.407662 8_2:-33602754 Msa1244660:CDS
AGCTCCCCTGTTCCGGCTGG+TGG 0.411187 8_2:-33603026 Msa1244660:CDS
ACCTGTTCCTTCCAGCCCAC+TGG 0.419128 8_2:+33603913 None:intergenic
TCCACCAACGAAATACTTGA+AGG 0.422626 8_2:+33603974 None:intergenic
GCCGCTCCAGCGGGGTCACC+AGG 0.425799 8_2:-33602729 Msa1244660:CDS
GCCGGAGGTGTGCTCACTAC+TGG 0.429088 8_2:+33602924 None:intergenic
ACCTCCGGCCGGTGGACCTA+TGG 0.433092 8_2:-33602910 Msa1244660:CDS
CACCAGGGGCACCAGGACCC+GGG 0.434025 8_2:-33602779 Msa1244660:CDS
GTCCTCCGGCTCCAGGACCC+GGG 0.434272 8_2:-33602815 Msa1244660:CDS
ATCTAATTCAACTGCACCAC+CGG 0.438406 8_2:-33602706 Msa1244660:CDS
AAAAGATCAAAACTGTGAAA+AGG 0.439027 8_2:-33603207 Msa1244660:CDS
AGCACCAACGGCAAGGGTTA+CGG 0.440891 8_2:+33602638 None:intergenic
GGATCAGGGACACCAGGACC+CGG 0.450156 8_2:-33602756 Msa1244660:CDS
GCCTTCAAGTATTTCGTTGG+TGG 0.451428 8_2:-33603975 Msa1244660:CDS
CCACCTGCTGGTGAAGGAGC+TGG 0.452499 8_2:+33602858 None:intergenic
AAAGATCAAAACTGTGAAAA+GGG 0.454501 8_2:-33603206 Msa1244660:CDS
GAGAATTACAACCAGTGGGC+TGG 0.458809 8_2:-33603924 Msa1244660:CDS
GAGCCGGAGGACCACCTGCT+GGG 0.459286 8_2:+33602826 None:intergenic
ACCTCCGGCTGGTGGACCTA+TGG 0.461437 8_2:-33602967 Msa1244660:CDS
TCTCCTTCACCAAGTGGTGG+TGG 0.464912 8_2:-33603134 Msa1244660:CDS
GCCGGAGGTGTGCTCACTAC+TGG 0.466620 8_2:+33602981 None:intergenic
GCAGGTGGTCCTCCGGCTCC+AGG 0.469719 8_2:-33602822 Msa1244660:CDS
GCAAGCTCCCCTGTTCCGGC+TGG 0.470059 8_2:-33603029 Msa1244660:CDS
ACCAGTGGGCTGGAAGGAAC+AGG 0.473167 8_2:-33603914 Msa1244660:CDS
GATCAGGGACACCAGGACCC+GGG 0.484770 8_2:-33602755 Msa1244660:CDS
CAAGTATTTCGTTGGTGGAA+AGG 0.487435 8_2:-33603970 Msa1244660:CDS
GGGGTAGATGGACCACCTGC+TGG 0.492892 8_2:+33602846 None:intergenic
TTTCCACTGATGAAATAGAA+TGG 0.497104 8_2:+33603227 None:intergenic
GCGACTCCACCAGGGGCACC+AGG 0.500033 8_2:-33602786 Msa1244660:CDS
TATGGCTTCATCTCCTTCTC+CGG 0.503605 8_2:-33602892 Msa1244660:CDS
GGAGCTGGTGGTCCACCTGC+CGG 0.504880 8_2:+33602873 None:intergenic
TATTTCGTTGGTGGAAAGGA+TGG 0.506955 8_2:-33603966 Msa1244660:CDS
GGTGGTCCACCTGCCGGAGA+AGG 0.507316 8_2:+33602879 None:intergenic
GAGTTCACTTTGGACAGAGC+AGG 0.508058 8_2:-33603251 Msa1244660:CDS
TCCTCCGGCTCCAGGACCCG+GGG 0.508521 8_2:-33602814 Msa1244660:CDS
GCTTCATCTCCTTCTCCGGC+AGG 0.510071 8_2:-33602888 Msa1244660:CDS
TTAGATCCTGGTGACCCCGC+TGG 0.511126 8_2:+33602723 None:intergenic
ATATTGAATTGAATGGATAT+AGG 0.511378 8_2:+33604075 None:intergenic
TACCCCAGCAGGTGGTCCTC+CGG 0.511478 8_2:-33602829 Msa1244660:CDS
CAAGCCTTCAAGTATTTCGT+TGG 0.517033 8_2:-33603978 Msa1244660:CDS
TCCAGTAGTGAGCACACCTC+CGG 0.521091 8_2:-33602925 Msa1244660:CDS
GTGAAGGATATTGAATTGAA+TGG 0.524878 8_2:+33604068 None:intergenic
CCACCAGCTCCTTCACCAGC+AGG 0.525764 8_2:-33602861 Msa1244660:CDS
TTCCAAATCAGTGACACTAT+AGG 0.526349 8_2:-33603891 Msa1244660:intron
CACTGGTTGTAATTCTCAGA+AGG 0.527393 8_2:+33603930 None:intergenic
GGTGGTCCATCTACCCCAGC+AGG 0.528795 8_2:-33602840 Msa1244660:CDS
CCAATCAAGAAATTCGAAGA+TGG 0.535933 8_2:-33603281 Msa1244660:CDS
ACCAGGGGCACCAGGACCCG+GGG 0.536686 8_2:-33602778 Msa1244660:CDS
GTGAAGAGAACAAAGAAGAG+AGG 0.543513 8_2:+33604020 None:intergenic
CTTGCTGGAGGAATAGCCGC+TGG 0.545733 8_2:+33603047 None:intergenic
TCAGGGACACCAGGACCCGG+GGG 0.547872 8_2:-33602753 Msa1244660:CDS
CTCACTACTGGAGATGGAGA+TGG 0.551978 8_2:+33602936 None:intergenic
CCGGGTCCTGGTGCCCCTGG+TGG 0.552029 8_2:+33602780 None:intergenic
GTGAGCACACCTCCGGCTGG+TGG 0.552623 8_2:-33602975 Msa1244660:CDS
TCCTGGTGACCCCGCTGGAG+CGG 0.558755 8_2:+33602728 None:intergenic
TCCAGTAGTGAGCACACCTC+CGG 0.562951 8_2:-33602982 Msa1244660:CDS
GGTGTGCTCACTACTGGAGA+TGG 0.563208 8_2:+33602930 None:intergenic
GGTGTGCTCACTACTGGAGA+TGG 0.563208 8_2:+33602987 None:intergenic
TGTTCCGGCTGGTGGACCTA+CGG 0.567370 8_2:-33603018 Msa1244660:CDS
TATTCCGTAACCCTTGCCGT+TGG 0.568597 8_2:-33602642 Msa1244660:CDS
TCATCTCCTTCTCCGGCAGG+TGG 0.568679 8_2:-33602885 Msa1244660:CDS
TAGGTCCACCAGCCGGAACA+GGG 0.568683 8_2:+33603021 None:intergenic
GGTCCATCTACCCCAGCAGG+TGG 0.568822 8_2:-33602837 Msa1244660:CDS
CTCCATAGCAAAATGTGTGA+AGG 0.568998 8_2:+33604052 None:intergenic
CCTGCTGGTGAAGGAGCTGG+TGG 0.574961 8_2:+33602861 None:intergenic
GGGAGCAGCGACTCCACCAG+GGG 0.575936 8_2:-33602793 Msa1244660:CDS
GGGCCATTCTATTTCATCAG+TGG 0.577208 8_2:-33603230 Msa1244660:CDS
GATGGACCACCTGCTGGTGA+AGG 0.578374 8_2:+33602852 None:intergenic
AGCCGGAGGACCACCTGCTG+GGG 0.579158 8_2:+33602827 None:intergenic
AGTTCACTTTGGACAGAGCA+GGG 0.582623 8_2:-33603250 Msa1244660:CDS
GGGGAGCAGCGACTCCACCA+GGG 0.582810 8_2:-33602794 Msa1244660:CDS
AGATGAAGCCATAGGTCCAC+CGG 0.584657 8_2:+33602902 None:intergenic
GTGAGCACACCTCCGGCCGG+TGG 0.585325 8_2:-33602918 Msa1244660:CDS
AAAACAGAGCACCAACGGCA+AGG 0.585538 8_2:+33602631 None:intergenic
AAACAGAGCACCAACGGCAA+GGG 0.586517 8_2:+33602632 None:intergenic
GTAGTGAGCACACCTCCGGC+TGG 0.586869 8_2:-33602978 Msa1244660:CDS
GGAGGAATAGCCGCTGGAGA+TGG 0.587071 8_2:+33603053 None:intergenic
GTTGTCGTTGGTGACGGTGA+AGG 0.587432 8_2:+33603101 None:intergenic
GGTGGTGAAACAGAGGGTGA+TGG 0.589277 8_2:+33603074 None:intergenic
ACTGGTTGTAATTCTCAGAA+GGG 0.589662 8_2:+33603931 None:intergenic
GGTGATGGAGTGGTTGTCGT+TGG 0.590401 8_2:+33603089 None:intergenic
TCGTTGGTGGAAAGGATGGA+TGG 0.594920 8_2:-33603962 Msa1244660:CDS
GCCATAGGTCCACCGGCCGG+AGG 0.595768 8_2:+33602909 None:intergenic
GGTGATAAGCCACCACCACT+TGG 0.595997 8_2:+33603125 None:intergenic
GGAGTGGTTGTCGTTGGTGA+CGG 0.597376 8_2:+33603095 None:intergenic
ACAAATGAACCACTGCTACC+CGG 0.604696 8_2:+33602687 None:intergenic
ATTACAACCAGTGGGCTGGA+AGG 0.611232 8_2:-33603920 Msa1244660:CDS
TGGTGATACAGAGTTCACTT+TGG 0.612356 8_2:-33603261 Msa1244660:CDS
GTCGTTGGTGACGGTGAAGG+TGG 0.613000 8_2:+33603104 None:intergenic
GAAGGGTTCAGACTCAGTGT+TGG 0.614528 8_2:-33603345 Msa1244660:CDS
GGAGATGGAGATGATGCCAT+AGG 0.617212 8_2:+33602951 None:intergenic
GAAGCCATAGGTCCACCGGC+CGG 0.617552 8_2:+33602906 None:intergenic
AAGCCACCACCACTTGGTGA+AGG 0.618943 8_2:+33603131 None:intergenic
TCTAATTCAACTGCACCACC+GGG 0.619239 8_2:-33602705 Msa1244660:CDS
TTCTGAGAATTACAACCAGT+GGG 0.619799 8_2:-33603928 Msa1244660:CDS
GCCATAGGTCCACCAGCCGG+AGG 0.620945 8_2:+33602966 None:intergenic
GGAACAGGGGAGCTTGCTGG+AGG 0.623639 8_2:+33603035 None:intergenic
AGGTCCACCAGCCGGAACAG+GGG 0.624119 8_2:+33603022 None:intergenic
GGTGCAGTTGAATTAGATCC+TGG 0.624618 8_2:+33602711 None:intergenic
ACTGCACCACCGGGTAGCAG+TGG 0.628398 8_2:-33602696 Msa1244660:CDS
TTCGTGGAGTTATAACAACA+AGG 0.629941 8_2:+33603171 None:intergenic
GATGCCATAGGTCCACCAGC+CGG 0.631786 8_2:+33602963 None:intergenic
CTTCTGAGAATTACAACCAG+TGG 0.634874 8_2:-33603929 Msa1244660:CDS
GGAGAAGGAGATGAAGCCAT+AGG 0.637297 8_2:+33602894 None:intergenic
CCAGCTCCTTCACCAGCAGG+TGG 0.640600 8_2:-33602858 Msa1244660:CDS
GTAGTGAGCACACCTCCGGC+CGG 0.648955 8_2:-33602921 Msa1244660:CDS
TGAAACAGAGGGTGATGGAG+TGG 0.649812 8_2:+33603079 None:intergenic
GATGCCGTAGGTCCACCAGC+CGG 0.653292 8_2:+33603014 None:intergenic
GGAATAGCCGCTGGAGATGG+TGG 0.653879 8_2:+33603056 None:intergenic
CCTCCGGCTCCAGGACCCGG+GGG 0.661807 8_2:-33602813 Msa1244660:CDS
GGAGATGGAGATGATGCCGT+AGG 0.662452 8_2:+33603002 None:intergenic
TCATCTCCTTCACCAAGTGG+TGG 0.662715 8_2:-33603137 Msa1244660:CDS
AAATCATCTCCTTCACCAAG+TGG 0.664193 8_2:-33603140 Msa1244660:CDS
TCGTGGAGTTATAACAACAA+GGG 0.673218 8_2:+33603172 None:intergenic
TGGAGATGGTGGTGAAACAG+AGG 0.697284 8_2:+33603067 None:intergenic
GAATACACCAGAAAGCTCGA+AGG 0.697343 8_2:+33602660 None:intergenic
CCAGGGGCACCAGGACCCGG+GGG 0.698232 8_2:-33602777 Msa1244660:CDS
GGAGATGGTGGTGAAACAGA+GGG 0.709029 8_2:+33603068 None:intergenic
AATGAACCACTGCTACCCGG+TGG 0.713699 8_2:+33602690 None:intergenic
TGTTTCACCACCATCTCCAG+CGG 0.715569 8_2:-33603063 Msa1244660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATAATAATAAAGTG+GGG + chr8_2:33603017-33603036 None:intergenic 10.0%
!!! GTATATATCAAATTAATTTT+TGG - chr8_2:33603177-33603196 Msa1244660:CDS 10.0%
!!! TTTAAGTTACAAAATTTTAT+AGG + chr8_2:33603440-33603459 None:intergenic 10.0%
!! ATTAAAATAATAATAAAGTG+GGG + chr8_2:33603017-33603036 None:intergenic 10.0%
!!! GTATATATCAAATTAATTTT+TGG - chr8_2:33603177-33603196 Msa1244660:CDS 10.0%
!!! TTTAAGTTACAAAATTTTAT+AGG + chr8_2:33603440-33603459 None:intergenic 10.0%
!! AAATCACAATAATTTCTTTA+TGG - chr8_2:33603280-33603299 Msa1244660:CDS 15.0%
!!! AAAATTTTATAGGAAAAACA+AGG + chr8_2:33603430-33603449 None:intergenic 15.0%
!! AAATCACAATAATTTCTTTA+TGG - chr8_2:33603280-33603299 Msa1244660:CDS 15.0%
!!! AAAATTTTATAGGAAAAACA+AGG + chr8_2:33603430-33603449 None:intergenic 15.0%
!!! AATTAAAAAGTACTATGTGA+TGG + chr8_2:33602743-33602762 None:intergenic 20.0%
!! ATATTGAATTGAATGGATAT+AGG + chr8_2:33602797-33602816 None:intergenic 20.0%
!!! ATCAAATTAATTTTTGGTCT+AGG - chr8_2:33603183-33603202 Msa1244660:CDS 20.0%
!!! TCAAATTAATTTTTGGTCTA+GGG - chr8_2:33603184-33603203 Msa1244660:CDS 20.0%
!!! TACGATCCTTTAATTTTTTA+TGG - chr8_2:33603375-33603394 Msa1244660:intron 20.0%
!! GAACAACCATAAAAAATTAA+AGG + chr8_2:33603384-33603403 None:intergenic 20.0%
!!! AATTAAAAAGTACTATGTGA+TGG + chr8_2:33602743-33602762 None:intergenic 20.0%
!! ATATTGAATTGAATGGATAT+AGG + chr8_2:33602797-33602816 None:intergenic 20.0%
!!! ATCAAATTAATTTTTGGTCT+AGG - chr8_2:33603183-33603202 Msa1244660:CDS 20.0%
!!! TCAAATTAATTTTTGGTCTA+GGG - chr8_2:33603184-33603203 Msa1244660:CDS 20.0%
!!! TACGATCCTTTAATTTTTTA+TGG - chr8_2:33603375-33603394 Msa1244660:intron 20.0%
!! GAACAACCATAAAAAATTAA+AGG + chr8_2:33603384-33603403 None:intergenic 20.0%
!! TATGTGATGGAAAAAACAAA+GGG + chr8_2:33602730-33602749 None:intergenic 25.0%
! AGTTGAATATGTGTTTAGTT+TGG - chr8_2:33603224-33603243 Msa1244660:CDS 25.0%
!!! TTAGTTTGGATTTTGTTGTT+TGG - chr8_2:33603238-33603257 Msa1244660:CDS 25.0%
!!! TATAGTTTTCAAGTACAAGA+AGG - chr8_2:33603506-33603525 Msa1244660:intron 25.0%
!!! ATAGTTTTCAAGTACAAGAA+GGG - chr8_2:33603507-33603526 Msa1244660:intron 25.0%
! AAAAGATCAAAACTGTGAAA+AGG - chr8_2:33603662-33603681 Msa1244660:intron 25.0%
! AAAGATCAAAACTGTGAAAA+GGG - chr8_2:33603663-33603682 Msa1244660:intron 25.0%
!! TATGTGATGGAAAAAACAAA+GGG + chr8_2:33602730-33602749 None:intergenic 25.0%
! AGTTGAATATGTGTTTAGTT+TGG - chr8_2:33603224-33603243 Msa1244660:CDS 25.0%
!!! TTAGTTTGGATTTTGTTGTT+TGG - chr8_2:33603238-33603257 Msa1244660:CDS 25.0%
!!! TATAGTTTTCAAGTACAAGA+AGG - chr8_2:33603506-33603525 Msa1244660:intron 25.0%
!!! ATAGTTTTCAAGTACAAGAA+GGG - chr8_2:33603507-33603526 Msa1244660:intron 25.0%
! AAAAGATCAAAACTGTGAAA+AGG - chr8_2:33603662-33603681 Msa1244660:intron 25.0%
! AAAGATCAAAACTGTGAAAA+GGG - chr8_2:33603663-33603682 Msa1244660:intron 25.0%
! CTATGTGATGGAAAAAACAA+AGG + chr8_2:33602731-33602750 None:intergenic 30.0%
GTGAAGGATATTGAATTGAA+TGG + chr8_2:33602804-33602823 None:intergenic 30.0%
CTATGTAACATACAACTACA+TGG - chr8_2:33603048-33603067 Msa1244660:CDS 30.0%
ATTAAAGGATCGTACAATAG+AGG + chr8_2:33603369-33603388 None:intergenic 30.0%
CATCTTCGAATTTCTTGATT+GGG + chr8_2:33603590-33603609 None:intergenic 30.0%
TTTCCACTGATGAAATAGAA+TGG + chr8_2:33603645-33603664 None:intergenic 30.0%
! CTATGTGATGGAAAAAACAA+AGG + chr8_2:33602731-33602750 None:intergenic 30.0%
GTGAAGGATATTGAATTGAA+TGG + chr8_2:33602804-33602823 None:intergenic 30.0%
CTATGTAACATACAACTACA+TGG - chr8_2:33603048-33603067 Msa1244660:CDS 30.0%
ATTAAAGGATCGTACAATAG+AGG + chr8_2:33603369-33603388 None:intergenic 30.0%
CATCTTCGAATTTCTTGATT+GGG + chr8_2:33603590-33603609 None:intergenic 30.0%
TTTCCACTGATGAAATAGAA+TGG + chr8_2:33603645-33603664 None:intergenic 30.0%
! ATCCTTCACACATTTTGCTA+TGG - chr8_2:33602815-33602834 Msa1244660:CDS 35.0%
ACTGGTTGTAATTCTCAGAA+GGG + chr8_2:33602941-33602960 None:intergenic 35.0%
! TTCTGAGAATTACAACCAGT+GGG - chr8_2:33602941-33602960 Msa1244660:CDS 35.0%
TTCCAAATCAGTGACACTAT+AGG - chr8_2:33602978-33602997 Msa1244660:CDS 35.0%
! TACCTATAGTGTCACTGATT+TGG + chr8_2:33602983-33603002 None:intergenic 35.0%
CCATCTTCGAATTTCTTGAT+TGG + chr8_2:33603591-33603610 None:intergenic 35.0%
CCAATCAAGAAATTCGAAGA+TGG - chr8_2:33603588-33603607 Msa1244660:intron 35.0%
TCGTGGAGTTATAACAACAA+GGG + chr8_2:33603700-33603719 None:intergenic 35.0%
TTCGTGGAGTTATAACAACA+AGG + chr8_2:33603701-33603720 None:intergenic 35.0%
! ATCCTTCACACATTTTGCTA+TGG - chr8_2:33602815-33602834 Msa1244660:CDS 35.0%
ACTGGTTGTAATTCTCAGAA+GGG + chr8_2:33602941-33602960 None:intergenic 35.0%
! TTCTGAGAATTACAACCAGT+GGG - chr8_2:33602941-33602960 Msa1244660:CDS 35.0%
TTCCAAATCAGTGACACTAT+AGG - chr8_2:33602978-33602997 Msa1244660:CDS 35.0%
! TACCTATAGTGTCACTGATT+TGG + chr8_2:33602983-33603002 None:intergenic 35.0%
CCATCTTCGAATTTCTTGAT+TGG + chr8_2:33603591-33603610 None:intergenic 35.0%
CCAATCAAGAAATTCGAAGA+TGG - chr8_2:33603588-33603607 Msa1244660:intron 35.0%
TCGTGGAGTTATAACAACAA+GGG + chr8_2:33603700-33603719 None:intergenic 35.0%
TTCGTGGAGTTATAACAACA+AGG + chr8_2:33603701-33603720 None:intergenic 35.0%
CTCCATAGCAAAATGTGTGA+AGG + chr8_2:33602820-33602839 None:intergenic 40.0%
GTGAAGAGAACAAAGAAGAG+AGG + chr8_2:33602852-33602871 None:intergenic 40.0%
CAAGCCTTCAAGTATTTCGT+TGG - chr8_2:33602891-33602910 Msa1244660:CDS 40.0%
TCCACCAACGAAATACTTGA+AGG + chr8_2:33602898-33602917 None:intergenic 40.0%
! CAAGTATTTCGTTGGTGGAA+AGG - chr8_2:33602899-33602918 Msa1244660:CDS 40.0%
! TATTTCGTTGGTGGAAAGGA+TGG - chr8_2:33602903-33602922 Msa1244660:CDS 40.0%
! CACTGGTTGTAATTCTCAGA+AGG + chr8_2:33602942-33602961 None:intergenic 40.0%
! CTTCTGAGAATTACAACCAG+TGG - chr8_2:33602940-33602959 Msa1244660:CDS 40.0%
TGGTGATACAGAGTTCACTT+TGG - chr8_2:33603608-33603627 Msa1244660:intron 40.0%
! CTTGGTGAAGGAGATGATTT+TGG + chr8_2:33603729-33603748 None:intergenic 40.0%
AAATCATCTCCTTCACCAAG+TGG - chr8_2:33603729-33603748 Msa1244660:intron 40.0%
ATCTAATTCAACTGCACCAC+CGG - chr8_2:33604163-33604182 Msa1244660:five_prime_UTR 40.0%
! ACTCAAAAACAGAGCACCAA+CGG + chr8_2:33604246-33604265 None:intergenic 40.0%
CTCCATAGCAAAATGTGTGA+AGG + chr8_2:33602820-33602839 None:intergenic 40.0%
GTGAAGAGAACAAAGAAGAG+AGG + chr8_2:33602852-33602871 None:intergenic 40.0%
CAAGCCTTCAAGTATTTCGT+TGG - chr8_2:33602891-33602910 Msa1244660:CDS 40.0%
TCCACCAACGAAATACTTGA+AGG + chr8_2:33602898-33602917 None:intergenic 40.0%
! CAAGTATTTCGTTGGTGGAA+AGG - chr8_2:33602899-33602918 Msa1244660:CDS 40.0%
! TATTTCGTTGGTGGAAAGGA+TGG - chr8_2:33602903-33602922 Msa1244660:CDS 40.0%
! CACTGGTTGTAATTCTCAGA+AGG + chr8_2:33602942-33602961 None:intergenic 40.0%
! CTTCTGAGAATTACAACCAG+TGG - chr8_2:33602940-33602959 Msa1244660:CDS 40.0%
TGGTGATACAGAGTTCACTT+TGG - chr8_2:33603608-33603627 Msa1244660:intron 40.0%
! CTTGGTGAAGGAGATGATTT+TGG + chr8_2:33603729-33603748 None:intergenic 40.0%
AAATCATCTCCTTCACCAAG+TGG - chr8_2:33603729-33603748 Msa1244660:intron 40.0%
ATCTAATTCAACTGCACCAC+CGG - chr8_2:33604163-33604182 Msa1244660:five_prime_UTR 40.0%
! ACTCAAAAACAGAGCACCAA+CGG + chr8_2:33604246-33604265 None:intergenic 40.0%
GCCTTCAAGTATTTCGTTGG+TGG - chr8_2:33602894-33602913 Msa1244660:CDS 45.0%
AGTTCACTTTGGACAGAGCA+GGG - chr8_2:33603619-33603638 Msa1244660:intron 45.0%
GGGCCATTCTATTTCATCAG+TGG - chr8_2:33603639-33603658 Msa1244660:intron 45.0%
!!! GATGATTTTGGTGGTGTTCG+TGG + chr8_2:33603717-33603736 None:intergenic 45.0%
! GGTGAAGGAGATGATTTTGG+TGG + chr8_2:33603726-33603745 None:intergenic 45.0%
TATGGCTTCATCTCCTTCTC+CGG - chr8_2:33603977-33603996 Msa1244660:CDS 45.0%
GGTGCAGTTGAATTAGATCC+TGG + chr8_2:33604161-33604180 None:intergenic 45.0%
TCTAATTCAACTGCACCACC+GGG - chr8_2:33604164-33604183 Msa1244660:five_prime_UTR 45.0%
ACAAATGAACCACTGCTACC+CGG + chr8_2:33604185-33604204 None:intergenic 45.0%
GAATACACCAGAAAGCTCGA+AGG + chr8_2:33604212-33604231 None:intergenic 45.0%
GCCTTCAAGTATTTCGTTGG+TGG - chr8_2:33602894-33602913 Msa1244660:CDS 45.0%
AGTTCACTTTGGACAGAGCA+GGG - chr8_2:33603619-33603638 Msa1244660:intron 45.0%
GGGCCATTCTATTTCATCAG+TGG - chr8_2:33603639-33603658 Msa1244660:intron 45.0%
!!! GATGATTTTGGTGGTGTTCG+TGG + chr8_2:33603717-33603736 None:intergenic 45.0%
! GGTGAAGGAGATGATTTTGG+TGG + chr8_2:33603726-33603745 None:intergenic 45.0%
TATGGCTTCATCTCCTTCTC+CGG - chr8_2:33603977-33603996 Msa1244660:CDS 45.0%
GGTGCAGTTGAATTAGATCC+TGG + chr8_2:33604161-33604180 None:intergenic 45.0%
TCTAATTCAACTGCACCACC+GGG - chr8_2:33604164-33604183 Msa1244660:five_prime_UTR 45.0%
ACAAATGAACCACTGCTACC+CGG + chr8_2:33604185-33604204 None:intergenic 45.0%
GAATACACCAGAAAGCTCGA+AGG + chr8_2:33604212-33604231 None:intergenic 45.0%
!! AATTAAAATAATAATAAAGT+GGG + chr8_2:33603018-33603037 None:intergenic 5.0%
!! AAATTAAAATAATAATAAAG+TGG + chr8_2:33603019-33603038 None:intergenic 5.0%
!!! ATTTTTTTTTATTTTTTAGA+AGG - chr8_2:33603113-33603132 Msa1244660:CDS 5.0%
!! AATTAAAATAATAATAAAGT+GGG + chr8_2:33603018-33603037 None:intergenic 5.0%
!! AAATTAAAATAATAATAAAG+TGG + chr8_2:33603019-33603038 None:intergenic 5.0%
!!! ATTTTTTTTTATTTTTTAGA+AGG - chr8_2:33603113-33603132 Msa1244660:CDS 5.0%
! TCGTTGGTGGAAAGGATGGA+TGG - chr8_2:33602907-33602926 Msa1244660:CDS 50.0%
GAGAATTACAACCAGTGGGC+TGG - chr8_2:33602945-33602964 Msa1244660:CDS 50.0%
!! ATTACAACCAGTGGGCTGGA+AGG - chr8_2:33602949-33602968 Msa1244660:CDS 50.0%
GAAGGGTTCAGACTCAGTGT+TGG - chr8_2:33603524-33603543 Msa1244660:intron 50.0%
GAGTTCACTTTGGACAGAGC+AGG - chr8_2:33603618-33603637 Msa1244660:intron 50.0%
TCATCTCCTTCACCAAGTGG+TGG - chr8_2:33603732-33603751 Msa1244660:intron 50.0%
!! TGAAACAGAGGGTGATGGAG+TGG + chr8_2:33603793-33603812 None:intergenic 50.0%
! GGAGATGGTGGTGAAACAGA+GGG + chr8_2:33603804-33603823 None:intergenic 50.0%
! TGGAGATGGTGGTGAAACAG+AGG + chr8_2:33603805-33603824 None:intergenic 50.0%
TGTTTCACCACCATCTCCAG+CGG - chr8_2:33603806-33603825 Msa1244660:intron 50.0%
GGAGATGGAGATGATGCCAT+AGG + chr8_2:33603921-33603940 None:intergenic 50.0%
CTCACTACTGGAGATGGAGA+TGG + chr8_2:33603879-33603898 None:intergenic 50.0%
AGATGAAGCCATAGGTCCAC+CGG + chr8_2:33603970-33603989 None:intergenic 50.0%
GGAGAAGGAGATGAAGCCAT+AGG + chr8_2:33603978-33603997 None:intergenic 50.0%
TGTAGCTCCTTCGAGCTTTC+TGG - chr8_2:33604202-33604221 Msa1244660:five_prime_UTR 50.0%
TATTCCGTAACCCTTGCCGT+TGG - chr8_2:33604227-33604246 Msa1244660:five_prime_UTR 50.0%
! AAACAGAGCACCAACGGCAA+GGG + chr8_2:33604240-33604259 None:intergenic 50.0%
! AAAACAGAGCACCAACGGCA+AGG + chr8_2:33604241-33604260 None:intergenic 50.0%
! TCGTTGGTGGAAAGGATGGA+TGG - chr8_2:33602907-33602926 Msa1244660:CDS 50.0%
GAGAATTACAACCAGTGGGC+TGG - chr8_2:33602945-33602964 Msa1244660:CDS 50.0%
!! ATTACAACCAGTGGGCTGGA+AGG - chr8_2:33602949-33602968 Msa1244660:CDS 50.0%
GAAGGGTTCAGACTCAGTGT+TGG - chr8_2:33603524-33603543 Msa1244660:intron 50.0%
GAGTTCACTTTGGACAGAGC+AGG - chr8_2:33603618-33603637 Msa1244660:intron 50.0%
TCATCTCCTTCACCAAGTGG+TGG - chr8_2:33603732-33603751 Msa1244660:intron 50.0%
!! TGAAACAGAGGGTGATGGAG+TGG + chr8_2:33603793-33603812 None:intergenic 50.0%
! GGAGATGGTGGTGAAACAGA+GGG + chr8_2:33603804-33603823 None:intergenic 50.0%
! TGGAGATGGTGGTGAAACAG+AGG + chr8_2:33603805-33603824 None:intergenic 50.0%
TGTTTCACCACCATCTCCAG+CGG - chr8_2:33603806-33603825 Msa1244660:intron 50.0%
GGAGATGGAGATGATGCCAT+AGG + chr8_2:33603921-33603940 None:intergenic 50.0%
CTCACTACTGGAGATGGAGA+TGG + chr8_2:33603879-33603898 None:intergenic 50.0%
AGATGAAGCCATAGGTCCAC+CGG + chr8_2:33603970-33603989 None:intergenic 50.0%
GGAGAAGGAGATGAAGCCAT+AGG + chr8_2:33603978-33603997 None:intergenic 50.0%
TGTAGCTCCTTCGAGCTTTC+TGG - chr8_2:33604202-33604221 Msa1244660:five_prime_UTR 50.0%
TATTCCGTAACCCTTGCCGT+TGG - chr8_2:33604227-33604246 Msa1244660:five_prime_UTR 50.0%
! AAACAGAGCACCAACGGCAA+GGG + chr8_2:33604240-33604259 None:intergenic 50.0%
! AAAACAGAGCACCAACGGCA+AGG + chr8_2:33604241-33604260 None:intergenic 50.0%
TCTCCTTCACCAAGTGGTGG+TGG - chr8_2:33603735-33603754 Msa1244660:intron 55.0%
! AAGCCACCACCACTTGGTGA+AGG + chr8_2:33603741-33603760 None:intergenic 55.0%
GGTGATAAGCCACCACCACT+TGG + chr8_2:33603747-33603766 None:intergenic 55.0%
!! GTTGTCGTTGGTGACGGTGA+AGG + chr8_2:33603771-33603790 None:intergenic 55.0%
! GGAGTGGTTGTCGTTGGTGA+CGG + chr8_2:33603777-33603796 None:intergenic 55.0%
!! GGTGATGGAGTGGTTGTCGT+TGG + chr8_2:33603783-33603802 None:intergenic 55.0%
GGTGGTGAAACAGAGGGTGA+TGG + chr8_2:33603798-33603817 None:intergenic 55.0%
GGAGATGGAGATGATGCCGT+AGG + chr8_2:33603870-33603889 None:intergenic 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_2:33603885-33603904 None:intergenic 55.0%
TCCAGTAGTGAGCACACCTC+CGG - chr8_2:33603887-33603906 Msa1244660:intron 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_2:33603885-33603904 None:intergenic 55.0%
TCCAGTAGTGAGCACACCTC+CGG - chr8_2:33603887-33603906 Msa1244660:intron 55.0%
AATGAACCACTGCTACCCGG+TGG + chr8_2:33604182-33604201 None:intergenic 55.0%
AGCACCAACGGCAAGGGTTA+CGG + chr8_2:33604234-33604253 None:intergenic 55.0%
TCTCCTTCACCAAGTGGTGG+TGG - chr8_2:33603735-33603754 Msa1244660:intron 55.0%
! AAGCCACCACCACTTGGTGA+AGG + chr8_2:33603741-33603760 None:intergenic 55.0%
GGTGATAAGCCACCACCACT+TGG + chr8_2:33603747-33603766 None:intergenic 55.0%
!! GTTGTCGTTGGTGACGGTGA+AGG + chr8_2:33603771-33603790 None:intergenic 55.0%
! GGAGTGGTTGTCGTTGGTGA+CGG + chr8_2:33603777-33603796 None:intergenic 55.0%
!! GGTGATGGAGTGGTTGTCGT+TGG + chr8_2:33603783-33603802 None:intergenic 55.0%
GGTGGTGAAACAGAGGGTGA+TGG + chr8_2:33603798-33603817 None:intergenic 55.0%
GGAGATGGAGATGATGCCGT+AGG + chr8_2:33603870-33603889 None:intergenic 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_2:33603885-33603904 None:intergenic 55.0%
TCCAGTAGTGAGCACACCTC+CGG - chr8_2:33603887-33603906 Msa1244660:intron 55.0%
GGTGTGCTCACTACTGGAGA+TGG + chr8_2:33603885-33603904 None:intergenic 55.0%
TCCAGTAGTGAGCACACCTC+CGG - chr8_2:33603887-33603906 Msa1244660:intron 55.0%
AATGAACCACTGCTACCCGG+TGG + chr8_2:33604182-33604201 None:intergenic 55.0%
AGCACCAACGGCAAGGGTTA+CGG + chr8_2:33604234-33604253 None:intergenic 55.0%
!! ACCAGTGGGCTGGAAGGAAC+AGG - chr8_2:33602955-33602974 Msa1244660:CDS 60.0%
ACCTGTTCCTTCCAGCCCAC+TGG + chr8_2:33602959-33602978 None:intergenic 60.0%
!! GTCGTTGGTGACGGTGAAGG+TGG + chr8_2:33603768-33603787 None:intergenic 60.0%
GGAATAGCCGCTGGAGATGG+TGG + chr8_2:33603816-33603835 None:intergenic 60.0%
GGAGGAATAGCCGCTGGAGA+TGG + chr8_2:33603819-33603838 None:intergenic 60.0%
CTTGCTGGAGGAATAGCCGC+TGG + chr8_2:33603825-33603844 None:intergenic 60.0%
TCCAGCAAGCTCCCCTGTTC+CGG - chr8_2:33603836-33603855 Msa1244660:intron 60.0%
TAGGTCCACCAGCCGGAACA+GGG + chr8_2:33603851-33603870 None:intergenic 60.0%
! TGTTCCGGCTGGTGGACCTA+CGG - chr8_2:33603851-33603870 Msa1244660:intron 60.0%
GATGCCATAGGTCCACCAGC+CGG + chr8_2:33603909-33603928 None:intergenic 60.0%
GCTTCATCTCCTTCTCCGGC+AGG - chr8_2:33603981-33604000 Msa1244660:CDS 60.0%
TCATCTCCTTCTCCGGCAGG+TGG - chr8_2:33603984-33604003 Msa1244660:CDS 60.0%
GATGGACCACCTGCTGGTGA+AGG + chr8_2:33604020-33604039 None:intergenic 60.0%
TTAGATCCTGGTGACCCCGC+TGG + chr8_2:33604149-33604168 None:intergenic 60.0%
!! ACCAGTGGGCTGGAAGGAAC+AGG - chr8_2:33602955-33602974 Msa1244660:CDS 60.0%
ACCTGTTCCTTCCAGCCCAC+TGG + chr8_2:33602959-33602978 None:intergenic 60.0%
!! GTCGTTGGTGACGGTGAAGG+TGG + chr8_2:33603768-33603787 None:intergenic 60.0%
GGAATAGCCGCTGGAGATGG+TGG + chr8_2:33603816-33603835 None:intergenic 60.0%
GGAGGAATAGCCGCTGGAGA+TGG + chr8_2:33603819-33603838 None:intergenic 60.0%
CTTGCTGGAGGAATAGCCGC+TGG + chr8_2:33603825-33603844 None:intergenic 60.0%
TCCAGCAAGCTCCCCTGTTC+CGG - chr8_2:33603836-33603855 Msa1244660:intron 60.0%
TAGGTCCACCAGCCGGAACA+GGG + chr8_2:33603851-33603870 None:intergenic 60.0%
! TGTTCCGGCTGGTGGACCTA+CGG - chr8_2:33603851-33603870 Msa1244660:intron 60.0%
GATGCCATAGGTCCACCAGC+CGG + chr8_2:33603909-33603928 None:intergenic 60.0%
GCTTCATCTCCTTCTCCGGC+AGG - chr8_2:33603981-33604000 Msa1244660:CDS 60.0%
TCATCTCCTTCTCCGGCAGG+TGG - chr8_2:33603984-33604003 Msa1244660:CDS 60.0%
GATGGACCACCTGCTGGTGA+AGG + chr8_2:33604020-33604039 None:intergenic 60.0%
TTAGATCCTGGTGACCCCGC+TGG + chr8_2:33604149-33604168 None:intergenic 60.0%
!! GGAACAGGGGAGCTTGCTGG+AGG + chr8_2:33603837-33603856 None:intergenic 65.0%
AGGTCCACCAGCCGGAACAG+GGG + chr8_2:33603850-33603869 None:intergenic 65.0%
GTAGGTCCACCAGCCGGAAC+AGG + chr8_2:33603852-33603871 None:intergenic 65.0%
GATGCCGTAGGTCCACCAGC+CGG + chr8_2:33603858-33603877 None:intergenic 65.0%
! GCCGGAGGTGTGCTCACTAC+TGG + chr8_2:33603891-33603910 None:intergenic 65.0%
GTAGTGAGCACACCTCCGGC+TGG - chr8_2:33603891-33603910 Msa1244660:intron 65.0%
! ACCTCCGGCTGGTGGACCTA+TGG - chr8_2:33603902-33603921 Msa1244660:CDS 65.0%
! GCCGGAGGTGTGCTCACTAC+TGG + chr8_2:33603891-33603910 None:intergenic 65.0%
GTAGTGAGCACACCTCCGGC+CGG - chr8_2:33603891-33603910 Msa1244660:intron 65.0%
GAAGCCATAGGTCCACCGGC+CGG + chr8_2:33603966-33603985 None:intergenic 65.0%
CCTGCTGGTGAAGGAGCTGG+TGG + chr8_2:33604011-33604030 None:intergenic 65.0%
CCACCAGCTCCTTCACCAGC+AGG - chr8_2:33604008-33604027 Msa1244660:CDS 65.0%
CCACCTGCTGGTGAAGGAGC+TGG + chr8_2:33604014-33604033 None:intergenic 65.0%
CCAGCTCCTTCACCAGCAGG+TGG - chr8_2:33604011-33604030 Msa1244660:CDS 65.0%
GGGGTAGATGGACCACCTGC+TGG + chr8_2:33604026-33604045 None:intergenic 65.0%
GGTGGTCCATCTACCCCAGC+AGG - chr8_2:33604029-33604048 Msa1244660:CDS 65.0%
GGTCCATCTACCCCAGCAGG+TGG - chr8_2:33604032-33604051 Msa1244660:CDS 65.0%
GGACCACCTGCTGGGGTAGA+TGG + chr8_2:33604038-33604057 None:intergenic 65.0%
TACCCCAGCAGGTGGTCCTC+CGG - chr8_2:33604040-33604059 Msa1244660:exon 65.0%
TCCTGGTGTCCCTGATCCCC+CGG + chr8_2:33604111-33604130 None:intergenic 65.0%
GGATCAGGGACACCAGGACC+CGG - chr8_2:33604113-33604132 Msa1244660:five_prime_UTR 65.0%
GATCAGGGACACCAGGACCC+GGG - chr8_2:33604114-33604133 Msa1244660:five_prime_UTR 65.0%
ATCAGGGACACCAGGACCCG+GGG - chr8_2:33604115-33604134 Msa1244660:five_prime_UTR 65.0%
ACTGCACCACCGGGTAGCAG+TGG - chr8_2:33604173-33604192 Msa1244660:five_prime_UTR 65.0%
!! GGAACAGGGGAGCTTGCTGG+AGG + chr8_2:33603837-33603856 None:intergenic 65.0%
AGGTCCACCAGCCGGAACAG+GGG + chr8_2:33603850-33603869 None:intergenic 65.0%
GTAGGTCCACCAGCCGGAAC+AGG + chr8_2:33603852-33603871 None:intergenic 65.0%
GATGCCGTAGGTCCACCAGC+CGG + chr8_2:33603858-33603877 None:intergenic 65.0%
! GCCGGAGGTGTGCTCACTAC+TGG + chr8_2:33603891-33603910 None:intergenic 65.0%
GTAGTGAGCACACCTCCGGC+TGG - chr8_2:33603891-33603910 Msa1244660:intron 65.0%
! ACCTCCGGCTGGTGGACCTA+TGG - chr8_2:33603902-33603921 Msa1244660:CDS 65.0%
! GCCGGAGGTGTGCTCACTAC+TGG + chr8_2:33603891-33603910 None:intergenic 65.0%
GTAGTGAGCACACCTCCGGC+CGG - chr8_2:33603891-33603910 Msa1244660:intron 65.0%
GAAGCCATAGGTCCACCGGC+CGG + chr8_2:33603966-33603985 None:intergenic 65.0%
CCTGCTGGTGAAGGAGCTGG+TGG + chr8_2:33604011-33604030 None:intergenic 65.0%
CCACCAGCTCCTTCACCAGC+AGG - chr8_2:33604008-33604027 Msa1244660:CDS 65.0%
CCACCTGCTGGTGAAGGAGC+TGG + chr8_2:33604014-33604033 None:intergenic 65.0%
CCAGCTCCTTCACCAGCAGG+TGG - chr8_2:33604011-33604030 Msa1244660:CDS 65.0%
GGGGTAGATGGACCACCTGC+TGG + chr8_2:33604026-33604045 None:intergenic 65.0%
GGTGGTCCATCTACCCCAGC+AGG - chr8_2:33604029-33604048 Msa1244660:CDS 65.0%
GGTCCATCTACCCCAGCAGG+TGG - chr8_2:33604032-33604051 Msa1244660:CDS 65.0%
GGACCACCTGCTGGGGTAGA+TGG + chr8_2:33604038-33604057 None:intergenic 65.0%
TACCCCAGCAGGTGGTCCTC+CGG - chr8_2:33604040-33604059 Msa1244660:exon 65.0%
TCCTGGTGTCCCTGATCCCC+CGG + chr8_2:33604111-33604130 None:intergenic 65.0%
GGATCAGGGACACCAGGACC+CGG - chr8_2:33604113-33604132 Msa1244660:five_prime_UTR 65.0%
GATCAGGGACACCAGGACCC+GGG - chr8_2:33604114-33604133 Msa1244660:five_prime_UTR 65.0%
ATCAGGGACACCAGGACCCG+GGG - chr8_2:33604115-33604134 Msa1244660:five_prime_UTR 65.0%
ACTGCACCACCGGGTAGCAG+TGG - chr8_2:33604173-33604192 Msa1244660:five_prime_UTR 65.0%
GCCGGAACAGGGGAGCTTGC+TGG + chr8_2:33603840-33603859 None:intergenic 70.0%
GCAAGCTCCCCTGTTCCGGC+TGG - chr8_2:33603840-33603859 Msa1244660:intron 70.0%
AGCTCCCCTGTTCCGGCTGG+TGG - chr8_2:33603843-33603862 Msa1244660:intron 70.0%
GTGAGCACACCTCCGGCTGG+TGG - chr8_2:33603894-33603913 Msa1244660:CDS 70.0%
GCCATAGGTCCACCAGCCGG+AGG + chr8_2:33603906-33603925 None:intergenic 70.0%
ACCTCCGGCCGGTGGACCTA+TGG - chr8_2:33603959-33603978 Msa1244660:CDS 70.0%
GGTGGTCCACCTGCCGGAGA+AGG + chr8_2:33603993-33604012 None:intergenic 70.0%
GGAGCTGGTGGTCCACCTGC+CGG + chr8_2:33603999-33604018 None:intergenic 70.0%
AGCCGGAGGACCACCTGCTG+GGG + chr8_2:33604045-33604064 None:intergenic 70.0%
GAGCCGGAGGACCACCTGCT+GGG + chr8_2:33604046-33604065 None:intergenic 70.0%
TGGTGGAGTCGCTGCTCCCC+CGG + chr8_2:33604075-33604094 None:intergenic 70.0%
GGGGAGCAGCGACTCCACCA+GGG - chr8_2:33604075-33604094 Msa1244660:five_prime_UTR 70.0%
GGGAGCAGCGACTCCACCAG+GGG - chr8_2:33604076-33604095 Msa1244660:five_prime_UTR 70.0%
CACCAGGACCCGGGGGATCA+GGG - chr8_2:33604099-33604118 Msa1244660:five_prime_UTR 70.0%
CCTGGTGTCCCTGATCCCCC+GGG + chr8_2:33604110-33604129 None:intergenic 70.0%
TCAGGGACACCAGGACCCGG+GGG - chr8_2:33604116-33604135 Msa1244660:five_prime_UTR 70.0%
TCCTGGTGACCCCGCTGGAG+CGG + chr8_2:33604144-33604163 None:intergenic 70.0%
GCCGGAACAGGGGAGCTTGC+TGG + chr8_2:33603840-33603859 None:intergenic 70.0%
GCAAGCTCCCCTGTTCCGGC+TGG - chr8_2:33603840-33603859 Msa1244660:intron 70.0%
AGCTCCCCTGTTCCGGCTGG+TGG - chr8_2:33603843-33603862 Msa1244660:intron 70.0%
GTGAGCACACCTCCGGCTGG+TGG - chr8_2:33603894-33603913 Msa1244660:CDS 70.0%
GCCATAGGTCCACCAGCCGG+AGG + chr8_2:33603906-33603925 None:intergenic 70.0%
ACCTCCGGCCGGTGGACCTA+TGG - chr8_2:33603959-33603978 Msa1244660:CDS 70.0%
GGTGGTCCACCTGCCGGAGA+AGG + chr8_2:33603993-33604012 None:intergenic 70.0%
GGAGCTGGTGGTCCACCTGC+CGG + chr8_2:33603999-33604018 None:intergenic 70.0%
AGCCGGAGGACCACCTGCTG+GGG + chr8_2:33604045-33604064 None:intergenic 70.0%
GAGCCGGAGGACCACCTGCT+GGG + chr8_2:33604046-33604065 None:intergenic 70.0%
TGGTGGAGTCGCTGCTCCCC+CGG + chr8_2:33604075-33604094 None:intergenic 70.0%
GGGGAGCAGCGACTCCACCA+GGG - chr8_2:33604075-33604094 Msa1244660:five_prime_UTR 70.0%
GGGAGCAGCGACTCCACCAG+GGG - chr8_2:33604076-33604095 Msa1244660:five_prime_UTR 70.0%
CACCAGGACCCGGGGGATCA+GGG - chr8_2:33604099-33604118 Msa1244660:five_prime_UTR 70.0%
CCTGGTGTCCCTGATCCCCC+GGG + chr8_2:33604110-33604129 None:intergenic 70.0%
TCAGGGACACCAGGACCCGG+GGG - chr8_2:33604116-33604135 Msa1244660:five_prime_UTR 70.0%
TCCTGGTGACCCCGCTGGAG+CGG + chr8_2:33604144-33604163 None:intergenic 70.0%
GTGAGCACACCTCCGGCCGG+TGG - chr8_2:33603951-33603970 Msa1244660:CDS 75.0%
GCCATAGGTCCACCGGCCGG+AGG + chr8_2:33603963-33603982 None:intergenic 75.0%
GGAGCCGGAGGACCACCTGC+TGG + chr8_2:33604047-33604066 None:intergenic 75.0%
GCAGGTGGTCCTCCGGCTCC+AGG - chr8_2:33604047-33604066 Msa1244660:exon 75.0%
GGTCCTCCGGCTCCAGGACC+CGG - chr8_2:33604053-33604072 Msa1244660:exon 75.0%
GTCCTCCGGCTCCAGGACCC+GGG - chr8_2:33604054-33604073 Msa1244660:exon 75.0%
TCCTCCGGCTCCAGGACCCG+GGG - chr8_2:33604055-33604074 Msa1244660:exon 75.0%
GGTGGAGTCGCTGCTCCCCC+GGG + chr8_2:33604074-33604093 None:intergenic 75.0%
GGGGGAGCAGCGACTCCACC+AGG - chr8_2:33604074-33604093 Msa1244660:five_prime_UTR 75.0%
!! GCGACTCCACCAGGGGCACC+AGG - chr8_2:33604083-33604102 Msa1244660:five_prime_UTR 75.0%
!! CCACCAGGGGCACCAGGACC+CGG - chr8_2:33604089-33604108 Msa1244660:five_prime_UTR 75.0%
!! CACCAGGGGCACCAGGACCC+GGG - chr8_2:33604090-33604109 Msa1244660:five_prime_UTR 75.0%
!! ACCAGGGGCACCAGGACCCG+GGG - chr8_2:33604091-33604110 Msa1244660:five_prime_UTR 75.0%
GCACCAGGACCCGGGGGATC+AGG - chr8_2:33604098-33604117 Msa1244660:five_prime_UTR 75.0%
GTCCCTGATCCCCCGGGTCC+TGG + chr8_2:33604104-33604123 None:intergenic 75.0%
CCCGGGGGATCAGGGACACC+AGG - chr8_2:33604107-33604126 Msa1244660:five_prime_UTR 75.0%
GTGAGCACACCTCCGGCCGG+TGG - chr8_2:33603951-33603970 Msa1244660:CDS 75.0%
GCCATAGGTCCACCGGCCGG+AGG + chr8_2:33603963-33603982 None:intergenic 75.0%
GGAGCCGGAGGACCACCTGC+TGG + chr8_2:33604047-33604066 None:intergenic 75.0%
GCAGGTGGTCCTCCGGCTCC+AGG - chr8_2:33604047-33604066 Msa1244660:exon 75.0%
GGTCCTCCGGCTCCAGGACC+CGG - chr8_2:33604053-33604072 Msa1244660:exon 75.0%
GTCCTCCGGCTCCAGGACCC+GGG - chr8_2:33604054-33604073 Msa1244660:exon 75.0%
TCCTCCGGCTCCAGGACCCG+GGG - chr8_2:33604055-33604074 Msa1244660:exon 75.0%
GGTGGAGTCGCTGCTCCCCC+GGG + chr8_2:33604074-33604093 None:intergenic 75.0%
GGGGGAGCAGCGACTCCACC+AGG - chr8_2:33604074-33604093 Msa1244660:five_prime_UTR 75.0%
!! GCGACTCCACCAGGGGCACC+AGG - chr8_2:33604083-33604102 Msa1244660:five_prime_UTR 75.0%
!! CCACCAGGGGCACCAGGACC+CGG - chr8_2:33604089-33604108 Msa1244660:five_prime_UTR 75.0%
!! CACCAGGGGCACCAGGACCC+GGG - chr8_2:33604090-33604109 Msa1244660:five_prime_UTR 75.0%
!! ACCAGGGGCACCAGGACCCG+GGG - chr8_2:33604091-33604110 Msa1244660:five_prime_UTR 75.0%
GCACCAGGACCCGGGGGATC+AGG - chr8_2:33604098-33604117 Msa1244660:five_prime_UTR 75.0%
GTCCCTGATCCCCCGGGTCC+TGG + chr8_2:33604104-33604123 None:intergenic 75.0%
CCCGGGGGATCAGGGACACC+AGG - chr8_2:33604107-33604126 Msa1244660:five_prime_UTR 75.0%
! CCTCCGGCTCCAGGACCCGG+GGG - chr8_2:33604056-33604075 Msa1244660:exon 80.0%
! GCTCCCCCGGGTCCTGGAGC+CGG + chr8_2:33604062-33604081 None:intergenic 80.0%
! GTCGCTGCTCCCCCGGGTCC+TGG + chr8_2:33604068-33604087 None:intergenic 80.0%
! CCGGGTCCTGGTGCCCCTGG+TGG + chr8_2:33604092-33604111 None:intergenic 80.0%
!!! CCAGGGGCACCAGGACCCGG+GGG - chr8_2:33604092-33604111 Msa1244660:five_prime_UTR 80.0%
! ACCCGGGGGAGCCGCTCCAG+CGG - chr8_2:33604130-33604149 Msa1244660:five_prime_UTR 80.0%
! GCCGCTCCAGCGGGGTCACC+AGG - chr8_2:33604140-33604159 Msa1244660:five_prime_UTR 80.0%
! CCTCCGGCTCCAGGACCCGG+GGG - chr8_2:33604056-33604075 Msa1244660:exon 80.0%
! GCTCCCCCGGGTCCTGGAGC+CGG + chr8_2:33604062-33604081 None:intergenic 80.0%
! GTCGCTGCTCCCCCGGGTCC+TGG + chr8_2:33604068-33604087 None:intergenic 80.0%
! CCGGGTCCTGGTGCCCCTGG+TGG + chr8_2:33604092-33604111 None:intergenic 80.0%
!!! CCAGGGGCACCAGGACCCGG+GGG - chr8_2:33604092-33604111 Msa1244660:five_prime_UTR 80.0%
! ACCCGGGGGAGCCGCTCCAG+CGG - chr8_2:33604130-33604149 Msa1244660:five_prime_UTR 80.0%
! GCCGCTCCAGCGGGGTCACC+AGG - chr8_2:33604140-33604159 Msa1244660:five_prime_UTR 80.0%
! CCCCCGGGTCCTGGAGCCGG+AGG + chr8_2:33604059-33604078 None:intergenic 85.0%
! CCCCCGGGTCCTGGTGCCCC+TGG + chr8_2:33604095-33604114 None:intergenic 85.0%
! GGAGCGGCTCCCCCGGGTCC+TGG + chr8_2:33604128-33604147 None:intergenic 85.0%
! CCCGCTGGAGCGGCTCCCCC+GGG + chr8_2:33604134-33604153 None:intergenic 85.0%
! CCCGGGGGAGCCGCTCCAGC+GGG - chr8_2:33604131-33604150 Msa1244660:five_prime_UTR 85.0%
! CCCCGCTGGAGCGGCTCCCC+CGG + chr8_2:33604135-33604154 None:intergenic 85.0%
! CCGGGGGAGCCGCTCCAGCG+GGG - chr8_2:33604132-33604151 Msa1244660:five_prime_UTR 85.0%
! CCCCCGGGTCCTGGAGCCGG+AGG + chr8_2:33604059-33604078 None:intergenic 85.0%
! CCCCCGGGTCCTGGTGCCCC+TGG + chr8_2:33604095-33604114 None:intergenic 85.0%
! GGAGCGGCTCCCCCGGGTCC+TGG + chr8_2:33604128-33604147 None:intergenic 85.0%
! CCCGCTGGAGCGGCTCCCCC+GGG + chr8_2:33604134-33604153 None:intergenic 85.0%
! CCCGGGGGAGCCGCTCCAGC+GGG - chr8_2:33604131-33604150 Msa1244660:five_prime_UTR 85.0%
! CCCCGCTGGAGCGGCTCCCC+CGG + chr8_2:33604135-33604154 None:intergenic 85.0%
! CCGGGGGAGCCGCTCCAGCG+GGG - chr8_2:33604132-33604151 Msa1244660:five_prime_UTR 85.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 33602620 33604271 33602620 ID=Msa1244660;Name=Msa1244660
chr8_2 mRNA 33602620 33604271 33602620 ID=Msa1244660-mRNA-1;Parent=Msa1244660;Name=Msa1244660-mRNA-1;_AED=0.01;_eAED=0.01;_QI=214|1|0.5|1|1|1|2|0|308
chr8_2 exon 33602620 33603380 33602620 ID=Msa1244660-mRNA-1:exon:10802;Parent=Msa1244660-mRNA-1
chr8_2 exon 33603892 33604271 33603892 ID=Msa1244660-mRNA-1:exon:10801;Parent=Msa1244660-mRNA-1
chr8_2 five_prime_UTR 33604058 33604271 33604058 ID=Msa1244660-mRNA-1:five_prime_utr;Parent=Msa1244660-mRNA-1
chr8_2 CDS 33603892 33604057 33603892 ID=Msa1244660-mRNA-1:cds;Parent=Msa1244660-mRNA-1
chr8_2 CDS 33602620 33603380 33602620 ID=Msa1244660-mRNA-1:cds;Parent=Msa1244660-mRNA-1
Gene Sequence

>Msa1244660

ATGGAGTTTAAGATACCTCTCTTCTTTGTTCTCTTCACTCTTGTTGTCTCAACTTCTCAAGCCTTCAAGTATTTCGTTGGTGGAAAGGATGGATGGACTCTAAACCCTTCTGAGAATTACAACCAGTGGGCTGGAAGGAACAGGTTCCAAATCAGTGACACTATAGTTTTCAAGTACAAGAAGGGTTCAGACTCAGTGTTGGAAGTGAAGAAAGAAGATTATGAAAAGTGCAACAAAACAAACCCAATCAAGAAATTCGAAGATGGTGATACAGAGTTCACTTTGGACAGAGCAGGGCCATTCTATTTCATCAGTGGAAAAGATCAAAACTGTGAAAAGGGTCAGAAATTAACCCTTGTTGTTATAACTCCACGAACACCACCAAAATCATCTCCTTCACCAAGTGGTGGTGGCTTATCACCACCTTCACCGTCACCAACGACAACCACTCCATCACCCTCTGTTTCACCACCATCTCCAGCGGCTATTCCTCCAGCAAGCTCCCCTGTTCCGGCTGGTGGACCTACGGCATCATCTCCATCTCCAGTAGTGAGCACACCTCCGGCTGGTGGACCTATGGCATCATCTCCATCTCCATCTCCAGTAGTGAGCACACCTCCGGCCGGTGGACCTATGGCTTCATCTCCTTCTCCGGCAGGTGGACCACCAGCTCCTTCACCAGCAGGTGGTCCATCTACCCCAGCAGGTGGTCCTCCGGCTCCAGGACCCGGGGGAGCAGCGACTCCACCAGGGGCACCAGGACCCGGGGGATCAGGGACACCAGGACCCGGGGGAGCCGCTCCAGCGGGGTCACCAGGATCTAATTCAACTGCACCACCGGGTAGCAGTGGTTCATTTGTAGCTCCTTCGAGCTTTCTGGTGTATTCCGTAACCCTTGCCGTTGGTGCTCTGTTTTTGAGTTACTGA
Protein sequence

>Msa1244660

MEFKIPLFFVLFTLVVSTSQAFKYFVGGKDGWTLNPSENYNQWAGRNRFQISDTIVFKYKKGSDSVLEVKKEDYEKCNKTNPIKKFEDGDTEFTLDRAGPFYFISGKDQNCEKGQKLTLVVITPRTPPKSSPSPSGGGLSPPSPSPTTTTPSPSVSPPSPAAIPPASSPVPAGGPTASSPSPVVSTPPAGGPMASSPSPSPVVSTPPAGGPMASSPSPAGGPPAPSPAGGPSTPAGGPPAPGPGGAATPPGAPGPGGSGTPGPGGAAPAGSPGSNSTAPPGSSGSFVAPSSFLVYSVTLAVGALFLSY*