AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0961230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0961230 MtrunA17_Chr6g0480011 99.689 322 1 0 1 322 1 322 0.0 666
Msa0961230 MtrunA17_Chr6g0480021 64.174 321 96 2 1 321 1 302 1.91e-151 427
Msa0961230 MtrunA17_Chr6g0479981 57.994 319 132 2 1 319 1 317 1.67e-135 387
Msa0961230 MtrunA17_Chr1g0173621 49.538 325 150 4 1 321 1 315 6.55e-107 314
Msa0961230 MtrunA17_Chr3g0121001 48.457 324 148 7 2 319 3 313 1.93e-97 290
Msa0961230 MtrunA17_Chr4g0036261 67.391 184 60 0 139 322 1 184 2.63e-91 270
Msa0961230 MtrunA17_Chr5g0447951 49.677 310 137 8 1 301 1 300 1.14e-87 265
Msa0961230 MtrunA17_Chr5g0447961 49.677 310 137 8 1 301 1 300 1.14e-87 265
Msa0961230 MtrunA17_Chr5g0447941 49.677 310 137 8 1 301 1 300 1.26e-87 265
Msa0961230 MtrunA17_Chr8g0368921 44.654 318 162 8 11 322 10 319 1.32e-87 265
Msa0961230 MtrunA17_Chr4g0018921 43.987 316 169 6 9 322 6 315 1.54e-86 262
Msa0961230 MtrunA17_Chr8g0368931 43.750 320 172 4 4 322 3 315 3.91e-84 256
Msa0961230 MtrunA17_Chr4g0036301 66.082 171 58 0 151 321 1 171 4.62e-81 243
Msa0961230 MtrunA17_Chr4g0036321 64.327 171 61 0 151 321 1 171 2.57e-77 234
Msa0961230 MtrunA17_Chr4g0009531 43.770 313 166 5 11 322 10 313 3.12e-76 236
Msa0961230 MtrunA17_Chr6g0479811 95.575 113 5 0 191 303 1 113 7.59e-75 225
Msa0961230 MtrunA17_Chr7g0239701 41.214 313 175 5 11 322 14 318 4.10e-70 220
Msa0961230 MtrunA17_Chr4g0009581 41.158 311 162 6 11 319 11 302 1.09e-66 211
Msa0961230 MtrunA17_Chr7g0243551 38.811 286 165 4 11 296 17 292 5.71e-64 204
Msa0961230 MtrunA17_Chr4g0036051 39.667 300 164 7 9 299 3 294 3.38e-59 192
Msa0961230 MtrunA17_Chr4g0009621 42.798 243 122 4 81 321 1 228 1.20e-51 170
Msa0961230 MtrunA17_Chr4g0036291 64.486 107 38 0 1 107 1 107 2.43e-46 152
Msa0961230 MtrunA17_Chr4g0036311 63.551 107 39 0 1 107 1 107 1.75e-45 150
Msa0961230 MtrunA17_Chr4g0036251 60.748 107 36 2 1 107 1 101 7.74e-39 133
Msa0961230 MtrunA17_Chr6g0479791 59.302 86 33 1 238 321 18 103 1.63e-28 106
Msa0961230 MtrunA17_Chr6g0479801 63.265 49 17 1 105 153 38 85 4.14e-15 69.7
Msa0961230 MtrunA17_Chr7g0274071 25.455 330 173 13 6 290 13 314 9.93e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0961230 AT1G59960.1 46.037 328 169 4 1 322 1 326 4.67e-107 315
Msa0961230 AT1G59950.1 48.065 310 157 3 15 322 13 320 1.51e-98 293
Msa0961230 AT2G37770.2 46.106 321 165 4 3 322 2 315 6.01e-92 276
Msa0961230 AT2G37790.1 46.006 313 160 4 11 322 10 314 1.10e-91 276
Msa0961230 AT2G37760.2 45.367 313 159 4 11 322 10 311 1.98e-86 262
Msa0961230 AT2G37760.1 45.367 313 159 4 11 322 10 311 1.98e-86 262
Msa0961230 AT5G62420.1 42.222 315 173 7 11 322 8 316 3.95e-86 262
Msa0961230 AT3G53880.1 44.728 313 165 4 11 322 10 315 8.72e-85 258
Msa0961230 AT2G37760.5 46.207 290 145 3 11 300 10 288 1.71e-82 251
Msa0961230 AT2G37760.4 45.993 287 144 3 11 297 10 285 3.07e-81 248
Msa0961230 AT2G37760.3 45.993 287 144 3 11 297 10 285 3.07e-81 248
Msa0961230 AT3G53880.2 44.561 285 151 3 11 295 10 287 1.78e-77 239
Msa0961230 AT5G01670.1 37.188 320 188 6 3 319 10 319 1.35e-74 233
Msa0961230 AT2G37760.6 45.802 262 137 3 62 322 8 265 7.29e-70 218
Msa0961230 AT2G37760.7 45.802 262 137 3 62 322 8 265 7.29e-70 218
Msa0961230 AT5G01670.2 34.294 347 188 7 3 319 10 346 1.23e-68 218
Msa0961230 AT2G21250.1 36.913 298 173 6 8 296 2 293 4.88e-63 202
Msa0961230 AT2G21260.1 36.700 297 173 6 8 295 2 292 9.21e-63 202
Msa0961230 AT2G37770.1 49.524 210 99 3 3 212 2 204 5.80e-62 199
Msa0961230 AT2G21250.2 36.134 238 137 6 8 236 2 233 3.80e-45 154
Msa0961230 AT2G21260.2 34.101 217 132 4 92 299 12 226 4.48e-38 135
Msa0961230 AT5G53580.1 24.172 302 170 10 21 286 64 342 1.69e-14 73.9

Find 92 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAGTCCTAATTATTAA+AGG 0.141053 6_3:-82336193 Msa0961230:three_prime_UTR
GATGATTGCTTTGCGTTTAT+TGG 0.158445 6_3:-82335981 Msa0961230:three_prime_UTR
CCATTGCACTTGATCCTATT+TGG 0.208388 6_3:+82336405 None:intergenic
TTCGATTCTGCTTCTGTCTA+TGG 0.228641 6_3:-82338898 Msa0961230:CDS
TTACTTCCCTTTGATATAGA+AGG 0.286868 6_3:-82337432 Msa0961230:CDS
CTTTAATAATTAGGACTTAG+AGG 0.290989 6_3:+82336194 None:intergenic
AAAGGGAAGTAAATCTTCTT+TGG 0.295146 6_3:+82337443 None:intergenic
GCGTTTATTGGTATGAAAAT+CGG 0.312139 6_3:-82335969 Msa0961230:three_prime_UTR
GTCAGCTCTTGGAGCTTATA+AGG 0.324007 6_3:-82336691 Msa0961230:CDS
TACAATGTTGTTTAGAAAGC+TGG 0.334762 6_3:-82337544 Msa0961230:intron
CTTTACAAAATATGCATGAT+TGG 0.345055 6_3:+82339102 None:intergenic
GACATGTGGCTATCTGAAAA+TGG 0.348870 6_3:-82336266 Msa0961230:CDS
ATGGAAGAGTGTTATAAGTT+AGG 0.361957 6_3:-82337396 Msa0961230:CDS
CATCACCCCTGCAGTGAATC+AGG 0.364569 6_3:-82337304 Msa0961230:intron
GCAAGACCTTAGAAGAGCTT+TGG 0.369881 6_3:-82336238 Msa0961230:CDS
TATAAGGTAACTTGGGGTTC+AGG 0.370516 6_3:-82336675 Msa0961230:CDS
ACTCAAAATCAAATATTTCA+AGG 0.375736 6_3:+82336348 None:intergenic
TCTATTGGCATATGCAACTA+TGG 0.381168 6_3:-82337363 Msa0961230:CDS
TCGATCTTTATCTGATTCAT+TGG 0.382694 6_3:-82337509 Msa0961230:CDS
CGTTTATTGGTATGAAAATC+GGG 0.391784 6_3:-82335968 Msa0961230:three_prime_UTR
CAAGACCTTAGAAGAGCTTT+GGG 0.402556 6_3:-82336237 Msa0961230:CDS
CTCAGGTGGGTGTACCAAAT+AGG 0.403459 6_3:-82336419 Msa0961230:CDS
TTAGGCTTAGCAAAGTCTAT+TGG 0.418231 6_3:-82337378 Msa0961230:CDS
TCCATCCCAAAGCTCTTCTA+AGG 0.422622 6_3:+82336232 None:intergenic
TCTTCTTTGGTAAATATGAC+AGG 0.431223 6_3:+82337456 None:intergenic
TTTGTTGATGCAATCAAAGT+TGG 0.432047 6_3:-82338931 Msa0961230:CDS
GGACACAAAATGCCAGTCAT+AGG 0.432511 6_3:-82339006 Msa0961230:CDS
CCTGAGTTCAACAAGACATC+TGG 0.440130 6_3:+82339027 None:intergenic
TGCATCAGTGTTCCATGGCT+TGG 0.441013 6_3:+82338792 None:intergenic
AGATCATGTCTCAGTCTTAC+CGG 0.441417 6_3:+82337486 None:intergenic
AGGTGGAGATGAATCCTTCT+TGG 0.442298 6_3:-82336770 Msa0961230:CDS
TGGAGCTTATAAGGTAACTT+GGG 0.443096 6_3:-82336682 Msa0961230:CDS
GAAGCAAGAACATCATTTGA+TGG 0.443280 6_3:+82338958 None:intergenic
GTTAGTGCATGGTCAGCTCT+TGG 0.451950 6_3:-82336702 Msa0961230:CDS
TTTAATGTGGTCTTAAGAGC+TGG 0.453099 6_3:+82338751 None:intergenic
CACAGCGAAGGCAATACTTA+GGG 0.453139 6_3:-82336289 Msa0961230:CDS
TTTGTGGTGATATGTGGTGG+TGG 0.454446 6_3:+82339156 None:intergenic
CCATGTCCCTTCTATATCAA+AGG 0.457170 6_3:+82337425 None:intergenic
CATCTATATAAAGTGGTTTG+TGG 0.464710 6_3:+82339140 None:intergenic
CAAGACATAGCAGCTGCAAA+AGG 0.471565 6_3:-82336627 Msa0961230:CDS
TTGGAGCTTATAAGGTAACT+TGG 0.473391 6_3:-82336683 Msa0961230:CDS
CTTGGCAGCAAGGGAAATTG+AGG 0.483207 6_3:-82336752 Msa0961230:CDS
TCTTTATCTGATTCATTGGC+CGG 0.484006 6_3:-82337505 Msa0961230:CDS
CCACAGCGAAGGCAATACTT+AGG 0.484368 6_3:-82336290 Msa0961230:CDS
AATTTCCCTTGCTGCCAAGA+AGG 0.487455 6_3:+82336756 None:intergenic
CATGTCCCTTCTATATCAAA+GGG 0.492063 6_3:+82337426 None:intergenic
GGCACTTCATCTATATAAAG+TGG 0.492167 6_3:+82339133 None:intergenic
AGACAGAAGCAGAATCGAAA+TGG 0.496929 6_3:+82338902 None:intergenic
AAATATGCATGATTGGCAAT+TGG 0.499219 6_3:+82339109 None:intergenic
CTGATTCACTGCAGGGGTGA+TGG 0.501160 6_3:+82337305 None:intergenic
TTGTGGTGATATGTGGTGGT+GGG 0.511412 6_3:+82339157 None:intergenic
TACTTCCCTTTGATATAGAA+GGG 0.517381 6_3:-82337431 Msa0961230:CDS
TATGTGTCGCCTCAGTTATG+TGG 0.521950 6_3:-82336064 Msa0961230:three_prime_UTR
TATGGAACAGAAGAGGCTAT+AGG 0.523988 6_3:-82338880 Msa0961230:CDS
ATACATACCTGATTCACTGC+AGG 0.528806 6_3:+82337297 None:intergenic
TTGGCAGCAAGGGAAATTGA+GGG 0.530461 6_3:-82336751 Msa0961230:CDS
AAAATGCCAGTCATAGGAAT+GGG 0.533740 6_3:-82339000 Msa0961230:CDS
CCAGATGTCTTGTTGAACTC+AGG 0.534183 6_3:-82339027 Msa0961230:CDS
ACAATGTTGTTTAGAAAGCT+GGG 0.535276 6_3:-82337543 Msa0961230:intron
TTGGGGTTCAGGCGCTGTCG+TGG 0.539309 6_3:-82336664 Msa0961230:CDS
ACCTTAGAAGAGCTTTGGGA+TGG 0.541491 6_3:-82336233 Msa0961230:CDS
ATTCATAGCTTACTTTAATG+TGG 0.541642 6_3:+82338738 None:intergenic
CCTTTGATATAGAAGGGACA+TGG 0.542358 6_3:-82337425 Msa0961230:CDS
CAAAATGCCAGTCATAGGAA+TGG 0.558036 6_3:-82339001 Msa0961230:CDS
AAGTGGTTTGTGGTGATATG+TGG 0.559147 6_3:+82339150 None:intergenic
AAGACATAGCAGCTGCAAAA+GGG 0.559376 6_3:-82336626 Msa0961230:CDS
GCTCTGCATATTTGTTCCAA+CGG 0.563455 6_3:-82336042 Msa0961230:three_prime_UTR
ATGAATCCTTCTTGGCAGCA+AGG 0.568216 6_3:-82336762 Msa0961230:CDS
CTTCTTTGGTAAATATGACA+GGG 0.570915 6_3:+82337457 None:intergenic
TACATACCTGATTCACTGCA+GGG 0.572609 6_3:+82337298 None:intergenic
GAAGTTCCCATTCCTATGAC+TGG 0.576432 6_3:+82338994 None:intergenic
AAAAGGGAAGACTACAGCTC+AGG 0.578206 6_3:-82336610 Msa0961230:intron
TGAATCCTTCTTGGCAGCAA+GGG 0.580141 6_3:-82336761 Msa0961230:CDS
AGAAGGGACATGGAAAGCAA+TGG 0.586366 6_3:-82337415 Msa0961230:CDS
GTTGTTTAGAAAGCTGGGAA+CGG 0.591717 6_3:-82337538 Msa0961230:intron
TCACACTCTTGTTCAACTCA+AGG 0.594406 6_3:+82336099 None:intergenic
TAATCTGCATCAGTGTTCCA+TGG 0.604256 6_3:+82338787 None:intergenic
GGAGCTTATAAGGTAACTTG+GGG 0.607696 6_3:-82336681 Msa0961230:CDS
AGAGGCTATAGGCATGGCTG+TGG 0.608547 6_3:-82338869 Msa0961230:CDS
AAGGAATTCATGTTAGTGCA+TGG 0.614427 6_3:-82336713 Msa0961230:CDS
GGCAATACTTAGGGGACATG+TGG 0.615565 6_3:-82336280 Msa0961230:CDS
AACAGAAGAGGCTATAGGCA+TGG 0.616765 6_3:-82338875 Msa0961230:CDS
TTAAGCAGATTCCACAGCGA+AGG 0.618267 6_3:-82336301 Msa0961230:CDS
TGGTTTGTGGTGATATGTGG+TGG 0.619909 6_3:+82339153 None:intergenic
TTCTGTCTATGGAACAGAAG+AGG 0.626511 6_3:-82338887 Msa0961230:CDS
TATGCATGATTGGCAATTGG+TGG 0.628915 6_3:+82339112 None:intergenic
CCTAAGTATTGCCTTCGCTG+TGG 0.629090 6_3:+82336290 None:intergenic
CCAAATAGGATCAAGTGCAA+TGG 0.633088 6_3:-82336405 Msa0961230:CDS
GTGGCAAAAGCTTTAGAACA+AGG 0.642750 6_3:-82338850 Msa0961230:CDS
ACAGCGAAGGCAATACTTAG+GGG 0.648697 6_3:-82336288 Msa0961230:CDS
TATGCAGAGCCACATAACTG+AGG 0.697612 6_3:+82336055 None:intergenic
ACATACCTGATTCACTGCAG+GGG 0.700439 6_3:+82337299 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAAACATGTTTATAAA+TGG - chr6_3:82338456-82338475 Msa0961230:intron 10.0%
!!! TTTATTTTCCTTTAATAATT+AGG + chr6_3:82338852-82338871 None:intergenic 10.0%
!!! TACTATATCTTTTACTTTTA+TGG - chr6_3:82336370-82336389 Msa0961230:CDS 15.0%
!!! TTTTGAATATCTTTTTATGT+TGG - chr6_3:82336475-82336494 Msa0961230:intron 15.0%
!! TCTTGAACAAATATTTAAAA+AGG - chr6_3:82337151-82337170 Msa0961230:intron 15.0%
!! CTTGAACAAATATTTAAAAA+GGG - chr6_3:82337152-82337171 Msa0961230:intron 15.0%
!! TTGAACAAATATTTAAAAAG+GGG - chr6_3:82337153-82337172 Msa0961230:intron 15.0%
!! AACATAAAATGTTATATCAA+AGG - chr6_3:82337219-82337238 Msa0961230:intron 15.0%
!! GACAAAAAAAAGTAAATTAA+CGG - chr6_3:82337404-82337423 Msa0961230:CDS 15.0%
!! ACAAAAAAAAGTAAATTAAC+GGG - chr6_3:82337405-82337424 Msa0961230:CDS 15.0%
!!! CTTTTTTCTATTTAATTCAA+AGG + chr6_3:82337830-82337849 None:intergenic 15.0%
!! CATTTCCAATATACATTATT+TGG - chr6_3:82336427-82336446 Msa0961230:CDS 20.0%
!! AATAGCCAAATAATGTATAT+TGG + chr6_3:82336435-82336454 None:intergenic 20.0%
!! AATATACATTATTTGGCTAT+TGG - chr6_3:82336434-82336453 Msa0961230:intron 20.0%
!!! TTGGAATTGATTTCTATAAT+AGG - chr6_3:82336494-82336513 Msa0961230:intron 20.0%
!! TTAAAACATTCATAAAGTCA+TGG + chr6_3:82336591-82336610 None:intergenic 20.0%
!!! ACTTTATGAATGTTTTAAGA+AGG - chr6_3:82336593-82336612 Msa0961230:intron 20.0%
!! ATTGTAAGTGGATAAATATA+GGG - chr6_3:82336678-82336697 Msa0961230:CDS 20.0%
!! TCAGTAAGTTGTTAAAATAT+GGG - chr6_3:82336710-82336729 Msa0961230:CDS 20.0%
!!! ATTTTGTGAATATTTCAAAC+TGG + chr6_3:82336799-82336818 None:intergenic 20.0%
!!! TTTGAAATATTCACAAAATC+AGG - chr6_3:82336800-82336819 Msa0961230:intron 20.0%
!! TTGAAATATTCACAAAATCA+GGG - chr6_3:82336801-82336820 Msa0961230:intron 20.0%
!!! TTTCAACTGATCAAATTTTA+CGG - chr6_3:82336919-82336938 Msa0961230:intron 20.0%
!!! ATAAAGTTGTTTTAAAACCA+TGG + chr6_3:82336989-82337008 None:intergenic 20.0%
!! AACTTTATTACAATAACGAA+AGG - chr6_3:82337001-82337020 Msa0961230:intron 20.0%
!! ATCGATTAAAAATAGCAATT+TGG - chr6_3:82338026-82338045 Msa0961230:intron 20.0%
!!! GATTTTTGTAAACATAGTTT+CGG - chr6_3:82338124-82338143 Msa0961230:intron 20.0%
!! TATATTAATTCATGTCATGA+TGG - chr6_3:82338563-82338582 Msa0961230:intron 20.0%
!!! CACATTATGATATAATTTTC+AGG - chr6_3:82338587-82338606 Msa0961230:intron 20.0%
!! ACTCAAAATCAAATATTTCA+AGG + chr6_3:82338689-82338708 None:intergenic 20.0%
!!! AATAAAAGAGTTTTATTCTC+AGG - chr6_3:82339017-82339036 Msa0961230:CDS 20.0%
! CTTTACAAAATATGCATGAT+TGG + chr6_3:82335935-82335954 None:intergenic 25.0%
! AACAAGAAGAAAGAAAAACA+TGG - chr6_3:82335970-82335989 Msa0961230:three_prime_UTR 25.0%
! ATTCATAGCTTACTTTAATG+TGG + chr6_3:82336299-82336318 None:intergenic 25.0%
! GATTGTAAGTGGATAAATAT+AGG - chr6_3:82336677-82336696 Msa0961230:CDS 25.0%
! GTCAGTAAGTTGTTAAAATA+TGG - chr6_3:82336709-82336728 Msa0961230:CDS 25.0%
! CAGTAAGTTGTTAAAATATG+GGG - chr6_3:82336711-82336730 Msa0961230:CDS 25.0%
! AGATATTAAAATCGAGTAGA+TGG + chr6_3:82336837-82336856 None:intergenic 25.0%
! TATTACGCAAATATTACACA+TGG - chr6_3:82336888-82336907 Msa0961230:intron 25.0%
! ATTTGATCAGTTGAAATTAG+CGG + chr6_3:82336915-82336934 None:intergenic 25.0%
!! TTCAACTGATCAAATTTTAC+GGG - chr6_3:82336920-82336939 Msa0961230:intron 25.0%
!! CAAAAAAGTGTATATTTCCA+TGG - chr6_3:82336969-82336988 Msa0961230:intron 25.0%
! TGAAATGTGGTATATTTACA+TGG - chr6_3:82337024-82337043 Msa0961230:intron 25.0%
! GGGAAAATATTGAAAACAAT+GGG - chr6_3:82337173-82337192 Msa0961230:intron 25.0%
! AAGGATTTAAGGAACAATAA+TGG + chr6_3:82337456-82337475 None:intergenic 25.0%
!!! CAAGAGTTTAGTCAATTTTT+TGG + chr6_3:82337699-82337718 None:intergenic 25.0%
! CAAAAAATTGACTAAACTCT+TGG - chr6_3:82337697-82337716 Msa0961230:intron 25.0%
! TCCTAATCATGTACAAAAAT+AGG + chr6_3:82337776-82337795 None:intergenic 25.0%
!!! GCTATTTTTAATCGATTACT+AGG + chr6_3:82338022-82338041 None:intergenic 25.0%
! TGTTTAGTGTACTTATTTAG+TGG + chr6_3:82338157-82338176 None:intergenic 25.0%
! TTTAATGGTTCATACAAAAC+TGG + chr6_3:82338186-82338205 None:intergenic 25.0%
! TGTATGAACCATTAAAAGTT+CGG - chr6_3:82338190-82338209 Msa0961230:intron 25.0%
!! GTTAACTTCAATTCTTTTTC+AGG - chr6_3:82338244-82338263 Msa0961230:intron 25.0%
! AGAAATGTTGTAAATCGTAA+GGG + chr6_3:82338519-82338538 None:intergenic 25.0%
! CTTTAATAATTAGGACTTAG+AGG + chr6_3:82338843-82338862 None:intergenic 25.0%
! CTCTAAGTCCTAATTATTAA+AGG - chr6_3:82338841-82338860 Msa0961230:CDS 25.0%
CATCTATATAAAGTGGTTTG+TGG + chr6_3:82335897-82335916 None:intergenic 30.0%
AAATATGCATGATTGGCAAT+TGG + chr6_3:82335928-82335947 None:intergenic 30.0%
TTTGTTGATGCAATCAAAGT+TGG - chr6_3:82336103-82336122 Msa0961230:three_prime_UTR 30.0%
TCAAAAGACCAAAAGAAACA+AGG + chr6_3:82336461-82336480 None:intergenic 30.0%
GTCCTTCAAAGATTATGTTA+GGG - chr6_3:82336646-82336665 Msa0961230:CDS 30.0%
TTCGAGAGTTTAATAGAATC+AGG - chr6_3:82336771-82336790 Msa0961230:CDS 30.0%
TCGAGAGTTTAATAGAATCA+GGG - chr6_3:82336772-82336791 Msa0961230:intron 30.0%
!! GACATCGATTTAAAACAATG+TGG + chr6_3:82337087-82337106 None:intergenic 30.0%
! TTAAATCGATGTCCTGTTTT+CGG - chr6_3:82337094-82337113 Msa0961230:intron 30.0%
GGGGAAAATATTGAAAACAA+TGG - chr6_3:82337172-82337191 Msa0961230:intron 30.0%
GACAACTTTGTAAACAAATG+TGG - chr6_3:82337195-82337214 Msa0961230:intron 30.0%
!! TTTTTAGGGAAAAGAAACCT+TGG + chr6_3:82337372-82337391 None:intergenic 30.0%
! GGTTTCTTTTCCCTAAAAAA+TGG - chr6_3:82337373-82337392 Msa0961230:CDS 30.0%
! TTCTTTTCCCTAAAAAATGG+AGG - chr6_3:82337376-82337395 Msa0961230:CDS 30.0%
!!! TCTCAATCCTCCATTTTTTA+GGG + chr6_3:82337386-82337405 None:intergenic 30.0%
AGAAGTTACACAAGGATTTA+AGG + chr6_3:82337467-82337486 None:intergenic 30.0%
TTGTACATAGAAGTTACACA+AGG + chr6_3:82337475-82337494 None:intergenic 30.0%
! TACAATGTTGTTTAGAAAGC+TGG - chr6_3:82337490-82337509 Msa0961230:CDS 30.0%
! ACAATGTTGTTTAGAAAGCT+GGG - chr6_3:82337491-82337510 Msa0961230:CDS 30.0%
TCGATCTTTATCTGATTCAT+TGG - chr6_3:82337525-82337544 Msa0961230:CDS 30.0%
CTTCTTTGGTAAATATGACA+GGG + chr6_3:82337580-82337599 None:intergenic 30.0%
TCTTCTTTGGTAAATATGAC+AGG + chr6_3:82337581-82337600 None:intergenic 30.0%
AAAGGGAAGTAAATCTTCTT+TGG + chr6_3:82337594-82337613 None:intergenic 30.0%
TTACTTCCCTTTGATATAGA+AGG - chr6_3:82337602-82337621 Msa0961230:intron 30.0%
TACTTCCCTTTGATATAGAA+GGG - chr6_3:82337603-82337622 Msa0961230:intron 30.0%
!! ATGGAAGAGTGTTATAAGTT+AGG - chr6_3:82337638-82337657 Msa0961230:intron 30.0%
!!! GCCTATTTTTGTACATGATT+AGG - chr6_3:82337772-82337791 Msa0961230:intron 30.0%
ATTGACGAACTAAACTGAAT+CGG - chr6_3:82337869-82337888 Msa0961230:intron 30.0%
AGTTCGTAAACATCACAATT+TGG - chr6_3:82337905-82337924 Msa0961230:intron 30.0%
AGCGTAAACAAATAGCAATT+TGG - chr6_3:82338052-82338071 Msa0961230:intron 30.0%
CAGTTCATAAAGATTCAGTT+TGG - chr6_3:82338082-82338101 Msa0961230:intron 30.0%
TTGTAAACATAGTTTCGGTT+CGG - chr6_3:82338129-82338148 Msa0961230:intron 30.0%
! AAACTGAACCGAACTTTTAA+TGG + chr6_3:82338201-82338220 None:intergenic 30.0%
! GTTTTATCGAACTGTTATGA+TGG - chr6_3:82338217-82338236 Msa0961230:intron 30.0%
!!! AACTTCAATTCTTTTTCAGG+TGG - chr6_3:82338247-82338266 Msa0961230:intron 30.0%
CAGAAATGTTGTAAATCGTA+AGG + chr6_3:82338520-82338539 None:intergenic 30.0%
! AATGGCAAAAAGCTTTAACA+AGG - chr6_3:82338647-82338666 Msa0961230:intron 30.0%
! CAAAAAGCTTTAACAAGGAA+AGG - chr6_3:82338652-82338671 Msa0961230:intron 30.0%
!! AAAACTCTTTTATTTGCCGT+TGG + chr6_3:82339011-82339030 None:intergenic 30.0%
GCGTTTATTGGTATGAAAAT+CGG - chr6_3:82339065-82339084 Msa0961230:exon 30.0%
CGTTTATTGGTATGAAAATC+GGG - chr6_3:82339066-82339085 Msa0961230:exon 30.0%
GGCACTTCATCTATATAAAG+TGG + chr6_3:82335904-82335923 None:intergenic 35.0%
AGAAGAAAGAAAAACATGGC+AGG - chr6_3:82335974-82335993 Msa0961230:three_prime_UTR 35.0%
AAAATGCCAGTCATAGGAAT+GGG - chr6_3:82336034-82336053 Msa0961230:three_prime_UTR 35.0%
GAAGCAAGAACATCATTTGA+TGG + chr6_3:82336079-82336098 None:intergenic 35.0%
! TTTAATGTGGTCTTAAGAGC+TGG + chr6_3:82336286-82336305 None:intergenic 35.0%
TGGAAATGAATGAAGACAGA+AGG + chr6_3:82336415-82336434 None:intergenic 35.0%
!! CTATTGGTCCTTGTTTCTTT+TGG - chr6_3:82336450-82336469 Msa0961230:intron 35.0%
!!! GTCATGGACTTTTTTGAAAG+CGG + chr6_3:82336575-82336594 None:intergenic 35.0%
GACCAAAACTAAAAGAGTCA+GGG + chr6_3:82336620-82336639 None:intergenic 35.0%
TGACCAAAACTAAAAGAGTC+AGG + chr6_3:82336621-82336640 None:intergenic 35.0%
CGTCCTTCAAAGATTATGTT+AGG - chr6_3:82336645-82336664 Msa0961230:CDS 35.0%
GTCCCTAACATAATCTTTGA+AGG + chr6_3:82336651-82336670 None:intergenic 35.0%
GGGACTAAATTGATTGTAAG+TGG - chr6_3:82336666-82336685 Msa0961230:CDS 35.0%
CAATAACGAAAGGTGAAATG+TGG - chr6_3:82337011-82337030 Msa0961230:intron 35.0%
CTTATACAAACACCGAAAAC+AGG + chr6_3:82337109-82337128 None:intergenic 35.0%
TATCAAAGGTTTGTGACCAA+TGG - chr6_3:82337233-82337252 Msa0961230:intron 35.0%
! GATTTTACCAAACAGTTCGT+CGG + chr6_3:82337284-82337303 None:intergenic 35.0%
! AATCCGAGTTCAAACTTGTT+TGG - chr6_3:82337300-82337319 Msa0961230:intron 35.0%
GTTCCAAACAAGTTTGAACT+CGG + chr6_3:82337306-82337325 None:intergenic 35.0%
GTAATTCAGATTTCCGTCAA+GGG + chr6_3:82337350-82337369 None:intergenic 35.0%
!!! GTCTCAATCCTCCATTTTTT+AGG + chr6_3:82337387-82337406 None:intergenic 35.0%
TCTTTATCTGATTCATTGGC+CGG - chr6_3:82337529-82337548 Msa0961230:CDS 35.0%
CATGTCCCTTCTATATCAAA+GGG + chr6_3:82337611-82337630 None:intergenic 35.0%
TTAGGCTTAGCAAAGTCTAT+TGG - chr6_3:82337656-82337675 Msa0961230:intron 35.0%
TCTATTGGCATATGCAACTA+TGG - chr6_3:82337671-82337690 Msa0961230:intron 35.0%
CTTTACTGTGATGTTCAGTT+CGG - chr6_3:82337930-82337949 Msa0961230:intron 35.0%
AAGGAATTCATGTTAGTGCA+TGG - chr6_3:82338321-82338340 Msa0961230:intron 35.0%
TTGGAGCTTATAAGGTAACT+TGG - chr6_3:82338351-82338370 Msa0961230:intron 35.0%
TGGAGCTTATAAGGTAACTT+GGG - chr6_3:82338352-82338371 Msa0961230:intron 35.0%
GGTCATGTAGATGATAAAAG+CGG - chr6_3:82338477-82338496 Msa0961230:intron 35.0%
!!! ATAATTTTCAGGTTGCACTC+AGG - chr6_3:82338598-82338617 Msa0961230:intron 35.0%
GATGATTGCTTTGCGTTTAT+TGG - chr6_3:82339053-82339072 Msa0961230:exon 35.0%
! AAGTGGTTTGTGGTGATATG+TGG + chr6_3:82335887-82335906 None:intergenic 40.0%
TATGCATGATTGGCAATTGG+TGG + chr6_3:82335925-82335944 None:intergenic 40.0%
CAAAATGCCAGTCATAGGAA+TGG - chr6_3:82336033-82336052 Msa0961230:three_prime_UTR 40.0%
AGACAGAAGCAGAATCGAAA+TGG + chr6_3:82336135-82336154 None:intergenic 40.0%
TTCGATTCTGCTTCTGTCTA+TGG - chr6_3:82336136-82336155 Msa0961230:three_prime_UTR 40.0%
TTCTGTCTATGGAACAGAAG+AGG - chr6_3:82336147-82336166 Msa0961230:three_prime_UTR 40.0%
TATGGAACAGAAGAGGCTAT+AGG - chr6_3:82336154-82336173 Msa0961230:three_prime_UTR 40.0%
!! GTGGCAAAAGCTTTAGAACA+AGG - chr6_3:82336184-82336203 Msa0961230:three_prime_UTR 40.0%
! TTTTCATCACTTCCAAGCCA+TGG - chr6_3:82336230-82336249 Msa0961230:CDS 40.0%
TAATCTGCATCAGTGTTCCA+TGG + chr6_3:82336250-82336269 None:intergenic 40.0%
TACAAAGTCAGGGACTAACT+TGG + chr6_3:82336550-82336569 None:intergenic 40.0%
AAGTTAGTCCCTGACTTTGT+AGG - chr6_3:82336549-82336568 Msa0961230:intron 40.0%
!!! GTCCCTGACTCTTTTAGTTT+TGG - chr6_3:82336615-82336634 Msa0961230:CDS 40.0%
! GGGACTGTACTGATAGTAAA+TGG - chr6_3:82336731-82336750 Msa0961230:CDS 40.0%
AGTCCTTGTACAGTCAGTAT+GGG + chr6_3:82336860-82336879 None:intergenic 40.0%
TAGTCCTTGTACAGTCAGTA+TGG + chr6_3:82336861-82336880 None:intergenic 40.0%
AACGATTTGAGCTCAACCAT+TGG + chr6_3:82337252-82337271 None:intergenic 40.0%
TAGACTTTAGCTACCCTTGA+CGG - chr6_3:82337334-82337353 Msa0961230:CDS 40.0%
GGTAATTCAGATTTCCGTCA+AGG + chr6_3:82337351-82337370 None:intergenic 40.0%
GACGGAAATCTGAATTACCA+AGG - chr6_3:82337352-82337371 Msa0961230:CDS 40.0%
GTTGTTTAGAAAGCTGGGAA+CGG - chr6_3:82337496-82337515 Msa0961230:CDS 40.0%
AGATCATGTCTCAGTCTTAC+CGG + chr6_3:82337551-82337570 None:intergenic 40.0%
CCATGTCCCTTCTATATCAA+AGG + chr6_3:82337612-82337631 None:intergenic 40.0%
CCTTTGATATAGAAGGGACA+TGG - chr6_3:82337609-82337628 Msa0961230:intron 40.0%
TACATACCTGATTCACTGCA+GGG + chr6_3:82337739-82337758 None:intergenic 40.0%
ATACATACCTGATTCACTGC+AGG + chr6_3:82337740-82337759 None:intergenic 40.0%
! GTTTACGAACTGAGTTGAAC+TGG + chr6_3:82337896-82337915 None:intergenic 40.0%
! GAAATTGAGGGAGTTTTGCA+AGG - chr6_3:82338295-82338314 Msa0961230:intron 40.0%
GGAGCTTATAAGGTAACTTG+GGG - chr6_3:82338353-82338372 Msa0961230:intron 40.0%
TATAAGGTAACTTGGGGTTC+AGG - chr6_3:82338359-82338378 Msa0961230:intron 40.0%
AAGACATAGCAGCTGCAAAA+GGG - chr6_3:82338408-82338427 Msa0961230:intron 40.0%
CCATTGCACTTGATCCTATT+TGG + chr6_3:82338632-82338651 None:intergenic 40.0%
CCAAATAGGATCAAGTGCAA+TGG - chr6_3:82338629-82338648 Msa0961230:intron 40.0%
GACATGTGGCTATCTGAAAA+TGG - chr6_3:82338768-82338787 Msa0961230:CDS 40.0%
CAAGACCTTAGAAGAGCTTT+GGG - chr6_3:82338797-82338816 Msa0961230:CDS 40.0%
TCACACTCTTGTTCAACTCA+AGG + chr6_3:82338938-82338957 None:intergenic 40.0%
GCTCTGCATATTTGTTCCAA+CGG - chr6_3:82338992-82339011 Msa0961230:CDS 40.0%
TTGTGGTGATATGTGGTGGT+GGG + chr6_3:82335880-82335899 None:intergenic 45.0%
TTTGTGGTGATATGTGGTGG+TGG + chr6_3:82335881-82335900 None:intergenic 45.0%
! TGGTTTGTGGTGATATGTGG+TGG + chr6_3:82335884-82335903 None:intergenic 45.0%
CCTGAGTTCAACAAGACATC+TGG + chr6_3:82336010-82336029 None:intergenic 45.0%
! CCAGATGTCTTGTTGAACTC+AGG - chr6_3:82336007-82336026 Msa0961230:three_prime_UTR 45.0%
GGACACAAAATGCCAGTCAT+AGG - chr6_3:82336028-82336047 Msa0961230:three_prime_UTR 45.0%
GAAGTTCCCATTCCTATGAC+TGG + chr6_3:82336043-82336062 None:intergenic 45.0%
AACAGAAGAGGCTATAGGCA+TGG - chr6_3:82336159-82336178 Msa0961230:three_prime_UTR 45.0%
! TCTCCCATACTGACTGTACA+AGG - chr6_3:82336854-82336873 Msa0961230:intron 45.0%
TGAGTATCCGACGAACTGTT+TGG - chr6_3:82337274-82337293 Msa0961230:intron 45.0%
AGAAGGGACATGGAAAGCAA+TGG - chr6_3:82337619-82337638 Msa0961230:intron 45.0%
ACATACCTGATTCACTGCAG+GGG + chr6_3:82337738-82337757 None:intergenic 45.0%
AGGTGGAGATGAATCCTTCT+TGG - chr6_3:82338264-82338283 Msa0961230:intron 45.0%
ATGAATCCTTCTTGGCAGCA+AGG - chr6_3:82338272-82338291 Msa0961230:intron 45.0%
TGAATCCTTCTTGGCAGCAA+GGG - chr6_3:82338273-82338292 Msa0961230:intron 45.0%
AATTTCCCTTGCTGCCAAGA+AGG + chr6_3:82338281-82338300 None:intergenic 45.0%
TTGGCAGCAAGGGAAATTGA+GGG - chr6_3:82338283-82338302 Msa0961230:intron 45.0%
! AGGGAGTTTTGCAAGGAGAA+AGG - chr6_3:82338302-82338321 Msa0961230:intron 45.0%
GTCAGCTCTTGGAGCTTATA+AGG - chr6_3:82338343-82338362 Msa0961230:intron 45.0%
CAAGACATAGCAGCTGCAAA+AGG - chr6_3:82338407-82338426 Msa0961230:intron 45.0%
AAAAGGGAAGACTACAGCTC+AGG - chr6_3:82338424-82338443 Msa0961230:intron 45.0%
!!! ATTTTCAGGTTGCACTCAGG+TGG - chr6_3:82338601-82338620 Msa0961230:intron 45.0%
!!! TTTTCAGGTTGCACTCAGGT+GGG - chr6_3:82338602-82338621 Msa0961230:intron 45.0%
TTAAGCAGATTCCACAGCGA+AGG - chr6_3:82338733-82338752 Msa0961230:intron 45.0%
CACAGCGAAGGCAATACTTA+GGG - chr6_3:82338745-82338764 Msa0961230:intron 45.0%
ACAGCGAAGGCAATACTTAG+GGG - chr6_3:82338746-82338765 Msa0961230:intron 45.0%
GCAAGACCTTAGAAGAGCTT+TGG - chr6_3:82338796-82338815 Msa0961230:CDS 45.0%
!! ACCTTAGAAGAGCTTTGGGA+TGG - chr6_3:82338801-82338820 Msa0961230:CDS 45.0%
TCCATCCCAAAGCTCTTCTA+AGG + chr6_3:82338805-82338824 None:intergenic 45.0%
TATGTGTCGCCTCAGTTATG+TGG - chr6_3:82338970-82338989 Msa0961230:CDS 45.0%
TATGCAGAGCCACATAACTG+AGG + chr6_3:82338982-82339001 None:intergenic 45.0%
CAAAGAATAATGCCTCGAGC+CGG - chr6_3:82339108-82339127 Msa0961230:five_prime_UTR 45.0%
! CCACCTATTGTGGTTCTATC+CGG + chr6_3:82339130-82339149 None:intergenic 45.0%
CGGATAGAACCACAATAGGT+GGG - chr6_3:82339128-82339147 Msa0961230:five_prime_UTR 45.0%
TGCATCAGTGTTCCATGGCT+TGG + chr6_3:82336245-82336264 None:intergenic 50.0%
GAAAGCGGCCTACAAAGTCA+GGG + chr6_3:82336560-82336579 None:intergenic 50.0%
TGAAAGCGGCCTACAAAGTC+AGG + chr6_3:82336561-82336580 None:intergenic 50.0%
CTTGGCAGCAAGGGAAATTG+AGG - chr6_3:82338282-82338301 Msa0961230:intron 50.0%
GTTAGTGCATGGTCAGCTCT+TGG - chr6_3:82338332-82338351 Msa0961230:intron 50.0%
CTCAGGTGGGTGTACCAAAT+AGG - chr6_3:82338615-82338634 Msa0961230:intron 50.0%
! CCTAAGTATTGCCTTCGCTG+TGG + chr6_3:82338747-82338766 None:intergenic 50.0%
CCACAGCGAAGGCAATACTT+AGG - chr6_3:82338744-82338763 Msa0961230:intron 50.0%
GGCAATACTTAGGGGACATG+TGG - chr6_3:82338754-82338773 Msa0961230:CDS 50.0%
GAGCCGGATAGAACCACAAT+AGG - chr6_3:82339124-82339143 Msa0961230:five_prime_UTR 50.0%
CCGGATAGAACCACAATAGG+TGG - chr6_3:82339127-82339146 Msa0961230:five_prime_UTR 50.0%
GAGAGCTTTCCCACCTATTG+TGG + chr6_3:82339140-82339159 None:intergenic 50.0%
AGAGGCTATAGGCATGGCTG+TGG - chr6_3:82336165-82336184 Msa0961230:three_prime_UTR 55.0%
CTGATTCACTGCAGGGGTGA+TGG + chr6_3:82337732-82337751 None:intergenic 55.0%
CATCACCCCTGCAGTGAATC+AGG - chr6_3:82337730-82337749 Msa0961230:intron 55.0%
! TTGTGGTTCTATCCGGCTCG+AGG + chr6_3:82339123-82339142 None:intergenic 55.0%
TGGGAAAGCTCTCGCTCAAG+AGG - chr6_3:82339147-82339166 Msa0961230:five_prime_UTR 55.0%
! TTGGGGTTCAGGCGCTGTCG+TGG - chr6_3:82338370-82338389 Msa0961230:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 82335866 82339190 82335866 ID=Msa0961230;Name=Msa0961230
chr6_3 mRNA 82335866 82339190 82335866 ID=Msa0961230-mRNA-1;Parent=Msa0961230;Name=Msa0961230-mRNA-1;_AED=0.32;_eAED=0.32;_QI=123|1|1|1|0.75|0.6|5|317|322
chr6_3 exon 82335866 82335889 82335866 ID=Msa0961230-mRNA-1:exon:14732;Parent=Msa0961230-mRNA-1
chr6_3 exon 82335930 82336447 82335930 ID=Msa0961230-mRNA-1:exon:14731;Parent=Msa0961230-mRNA-1
chr6_3 exon 82336611 82336790 82336611 ID=Msa0961230-mRNA-1:exon:14730;Parent=Msa0961230-mRNA-1
chr6_3 exon 82337305 82337551 82337305 ID=Msa0961230-mRNA-1:exon:14729;Parent=Msa0961230-mRNA-1
chr6_3 exon 82338751 82339190 82338751 ID=Msa0961230-mRNA-1:exon:14728;Parent=Msa0961230-mRNA-1
chr6_3 five_prime_UTR 82339068 82339190 82339068 ID=Msa0961230-mRNA-1:five_prime_utr;Parent=Msa0961230-mRNA-1
chr6_3 CDS 82338751 82339067 82338751 ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3 CDS 82337305 82337551 82337305 ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3 CDS 82336611 82336790 82336611 ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3 CDS 82336223 82336447 82336223 ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3 three_prime_UTR 82335930 82336222 82335930 ID=Msa0961230-mRNA-1:three_prime_utr;Parent=Msa0961230-mRNA-1
chr6_3 three_prime_UTR 82335866 82335889 82335866 ID=Msa0961230-mRNA-1:three_prime_utr;Parent=Msa0961230-mRNA-1
Gene Sequence

>Msa0961230

ATGGCAGGAAACAAAATTCCAGATGTCTTGTTGAACTCAGGACACAAAATGCCAGTCATAGGAATGGGAACTTCAGTAGACAATCGTCCATCAAATGATGTTCTTGCTTCTATCTTTGTTGATGCAATCAAAGTTGGTTATCGCCATTTCGATTCTGCTTCTGTCTATGGAACAGAAGAGGCTATAGGCATGGCTGTGGCAAAAGCTTTAGAACAAGGTCTTATTAAGAGTAGAGATGAACTTTTCATCACTTCCAAGCCATGGAACACTGATGCAGATTATGATCTTATTGTTCCAGCTCTTAAGACCACATTAAAAAAGCTGGGAACGGAGTATGTCGATCTTTATCTGATTCATTGGCCGGTAAGACTGAGACATGATCTTGAAAACCCTGTCATATTTACCAAAGAAGATTTACTTCCCTTTGATATAGAAGGGACATGGAAAGCAATGGAAGAGTGTTATAAGTTAGGCTTAGCAAAGTCTATTGGCATATGCAACTATGGTACCAAAAAATTGACTAAACTCTTGGAAACTGCTACCATCACCCCTGCAGTGAATCAGGTGGAGATGAATCCTTCTTGGCAGCAAGGGAAATTGAGGGAGTTTTGCAAGGAGAAAGGAATTCATGTTAGTGCATGGTCAGCTCTTGGAGCTTATAAGGTAACTTGGGGTTCAGGCGCTGTCGTGGAGAATCAAATTCTTCAAGACATAGCAGCTGCAAAAGGGAAGACTACAGCTCAGGTTGCACTCAGGTGGGTGTACCAAATAGGATCAAGTGCAATGGCAAAAAGCTTTAACAAGGAAAGGATGACACAAAACCTTGAAATATTTGATTTTGAGTTGAGTGAAGATGACTTAGAGAAGATTAAGCAGATTCCACAGCGAAGGCAATACTTAGGGGACATGTGGCTATCTGAAAATGGATCATGCAAGACCTTAGAAGAGCTTTGGGATGGAGATGTTTGA
Protein sequence

>Msa0961230

MAGNKIPDVLLNSGHKMPVIGMGTSVDNRPSNDVLASIFVDAIKVGYRHFDSASVYGTEEAIGMAVAKALEQGLIKSRDELFITSKPWNTDADYDLIVPALKTTLKKLGTEYVDLYLIHWPVRLRHDLENPVIFTKEDLLPFDIEGTWKAMEECYKLGLAKSIGICNYGTKKLTKLLETATITPAVNQVEMNPSWQQGKLREFCKEKGIHVSAWSALGAYKVTWGSGAVVENQILQDIAAAKGKTTAQVALRWVYQIGSSAMAKSFNKERMTQNLEIFDFELSEDDLEKIKQIPQRRQYLGDMWLSENGSCKTLEELWDGDV*