AlfalfaGEDB | Msa0961230

M. sativa cultivar zhongmu-4 / Msa0961230

KEGG: K00002|K00011|K08243|K22374
GO: -
PFAM: PF00248.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961230	sp\|A0A2P1GIY9\|REDX2_CATRO	60.312	320	123	3	5	322	6	323	1.66e-141	405

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961230	A0A396HGB9	99.689	322	1	0	1	322	1	322	0.0	666

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0026670	Msa0961230	0.813959	1.964980e-51	-8.615850e-47
Msa0087130	Msa0961230	0.813845	2.082930e-51	-8.615850e-47
Msa0109260	Msa0961230	0.831734	1.411024e-55	-8.615850e-47
Msa0133450	Msa0961230	0.814360	1.602719e-51	-8.615850e-47
Msa0230980	Msa0961230	0.801107	1.052521e-48	-8.615850e-47
Msa0270460	Msa0961230	0.800104	1.685345e-48	-8.615850e-47
Msa0272990	Msa0961230	0.808793	2.600817e-50	-8.615850e-47
Msa0355990	Msa0961230	0.806761	7.029174e-50	-8.615850e-47
Msa0367590	Msa0961230	0.814091	1.838168e-51	-8.615850e-47
Msa0373110	Msa0961230	0.839426	1.595754e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961230	MtrunA17_Chr6g0480011	99.689	322	1	0	1	322	1	322	0.0	666
Msa0961230	MtrunA17_Chr6g0480021	64.174	321	96	2	1	321	1	302	1.91e-151	427
Msa0961230	MtrunA17_Chr6g0479981	57.994	319	132	2	1	319	1	317	1.67e-135	387
Msa0961230	MtrunA17_Chr1g0173621	49.538	325	150	4	1	321	1	315	6.55e-107	314
Msa0961230	MtrunA17_Chr3g0121001	48.457	324	148	7	2	319	3	313	1.93e-97	290
Msa0961230	MtrunA17_Chr4g0036261	67.391	184	60	0	139	322	1	184	2.63e-91	270
Msa0961230	MtrunA17_Chr5g0447951	49.677	310	137	8	1	301	1	300	1.14e-87	265
Msa0961230	MtrunA17_Chr5g0447961	49.677	310	137	8	1	301	1	300	1.14e-87	265
Msa0961230	MtrunA17_Chr5g0447941	49.677	310	137	8	1	301	1	300	1.26e-87	265
Msa0961230	MtrunA17_Chr8g0368921	44.654	318	162	8	11	322	10	319	1.32e-87	265

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0961230	AT1G59960.1	46.037	328	169	4	1	322	1	326	4.67e-107	315
Msa0961230	AT1G59950.1	48.065	310	157	3	15	322	13	320	1.51e-98	293
Msa0961230	AT2G37770.2	46.106	321	165	4	3	322	2	315	6.01e-92	276
Msa0961230	AT2G37790.1	46.006	313	160	4	11	322	10	314	1.10e-91	276
Msa0961230	AT2G37760.2	45.367	313	159	4	11	322	10	311	1.98e-86	262
Msa0961230	AT2G37760.1	45.367	313	159	4	11	322	10	311	1.98e-86	262
Msa0961230	AT5G62420.1	42.222	315	173	7	11	322	8	316	3.95e-86	262
Msa0961230	AT3G53880.1	44.728	313	165	4	11	322	10	315	8.72e-85	258
Msa0961230	AT2G37760.5	46.207	290	145	3	11	300	10	288	1.71e-82	251
Msa0961230	AT2G37760.4	45.993	287	144	3	11	297	10	285	3.07e-81	248

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Find 92 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGAAGACTACAGCTC+AGG	0.578206	6_3:-82336610	Msa0961230:intron
AAAATGCCAGTCATAGGAAT+GGG	0.533740	6_3:-82339000	Msa0961230:CDS
AAAGGGAAGTAAATCTTCTT+TGG	0.295146	6_3:+82337443	None:intergenic
AAATATGCATGATTGGCAAT+TGG	0.499219	6_3:+82339109	None:intergenic
AACAGAAGAGGCTATAGGCA+TGG	0.616765	6_3:-82338875	Msa0961230:CDS
AAGACATAGCAGCTGCAAAA+GGG	0.559376	6_3:-82336626	Msa0961230:CDS
AAGGAATTCATGTTAGTGCA+TGG	0.614427	6_3:-82336713	Msa0961230:CDS
AAGTGGTTTGTGGTGATATG+TGG	0.559147	6_3:+82339150	None:intergenic
AATTTCCCTTGCTGCCAAGA+AGG	0.487455	6_3:+82336756	None:intergenic
ACAATGTTGTTTAGAAAGCT+GGG	0.535276	6_3:-82337543	Msa0961230:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATCTATATAAAGTGGTTTG+TGG	+	chr6_3:82335897-82335916	None:intergenic	30.0%
AAATATGCATGATTGGCAAT+TGG	+	chr6_3:82335928-82335947	None:intergenic	30.0%
TTTGTTGATGCAATCAAAGT+TGG	-	chr6_3:82336103-82336122	Msa0961230:three_prime_UTR	30.0%
TCAAAAGACCAAAAGAAACA+AGG	+	chr6_3:82336461-82336480	None:intergenic	30.0%
GTCCTTCAAAGATTATGTTA+GGG	-	chr6_3:82336646-82336665	Msa0961230:CDS	30.0%
TTCGAGAGTTTAATAGAATC+AGG	-	chr6_3:82336771-82336790	Msa0961230:CDS	30.0%
TCGAGAGTTTAATAGAATCA+GGG	-	chr6_3:82336772-82336791	Msa0961230:intron	30.0%
GGGGAAAATATTGAAAACAA+TGG	-	chr6_3:82337172-82337191	Msa0961230:intron	30.0%
GACAACTTTGTAAACAAATG+TGG	-	chr6_3:82337195-82337214	Msa0961230:intron	30.0%
AGAAGTTACACAAGGATTTA+AGG	+	chr6_3:82337467-82337486	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	82335866	82339190	82335866	ID=Msa0961230;Name=Msa0961230
chr6_3	mRNA	82335866	82339190	82335866	ID=Msa0961230-mRNA-1;Parent=Msa0961230;Name=Msa0961230-mRNA-1;_AED=0.32;_eAED=0.32;_QI=123\|1\|1\|1\|0.75\|0.6\|5\|317\|322
chr6_3	exon	82335866	82335889	82335866	ID=Msa0961230-mRNA-1:exon:14732;Parent=Msa0961230-mRNA-1
chr6_3	exon	82335930	82336447	82335930	ID=Msa0961230-mRNA-1:exon:14731;Parent=Msa0961230-mRNA-1
chr6_3	exon	82336611	82336790	82336611	ID=Msa0961230-mRNA-1:exon:14730;Parent=Msa0961230-mRNA-1
chr6_3	exon	82337305	82337551	82337305	ID=Msa0961230-mRNA-1:exon:14729;Parent=Msa0961230-mRNA-1
chr6_3	exon	82338751	82339190	82338751	ID=Msa0961230-mRNA-1:exon:14728;Parent=Msa0961230-mRNA-1
chr6_3	five_prime_UTR	82339068	82339190	82339068	ID=Msa0961230-mRNA-1:five_prime_utr;Parent=Msa0961230-mRNA-1
chr6_3	CDS	82338751	82339067	82338751	ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3	CDS	82337305	82337551	82337305	ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3	CDS	82336611	82336790	82336611	ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3	CDS	82336223	82336447	82336223	ID=Msa0961230-mRNA-1:cds;Parent=Msa0961230-mRNA-1
chr6_3	three_prime_UTR	82335930	82336222	82335930	ID=Msa0961230-mRNA-1:three_prime_utr;Parent=Msa0961230-mRNA-1
chr6_3	three_prime_UTR	82335866	82335889	82335866	ID=Msa0961230-mRNA-1:three_prime_utr;Parent=Msa0961230-mRNA-1

Gene Sequence

>Msa0961230

ATGGCAGGAAACAAAATTCCAGATGTCTTGTTGAACTCAGGACACAAAATGCCAGTCATAGGAATGGGAACTTCAGTAGACAATCGTCCATCAAATGATGTTCTTGCTTCTATCTTTGTTGATGCAATCAAAGTTGGTTATCGCCATTTCGATTCTGCTTCTGTCTATGGAACAGAAGAGGCTATAGGCATGGCTGTGGCAAAAGCTTTAGAACAAGGTCTTATTAAGAGTAGAGATGAACTTTTCATCACTTCCAAGCCATGGAACACTGATGCAGATTATGATCTTATTGTTCCAGCTCTTAAGACCACATTAAAAAAGCTGGGAACGGAGTATGTCGATCTTTATCTGATTCATTGGCCGGTAAGACTGAGACATGATCTTGAAAACCCTGTCATATTTACCAAAGAAGATTTACTTCCCTTTGATATAGAAGGGACATGGAAAGCAATGGAAGAGTGTTATAAGTTAGGCTTAGCAAAGTCTATTGGCATATGCAACTATGGTACCAAAAAATTGACTAAACTCTTGGAAACTGCTACCATCACCCCTGCAGTGAATCAGGTGGAGATGAATCCTTCTTGGCAGCAAGGGAAATTGAGGGAGTTTTGCAAGGAGAAAGGAATTCATGTTAGTGCATGGTCAGCTCTTGGAGCTTATAAGGTAACTTGGGGTTCAGGCGCTGTCGTGGAGAATCAAATTCTTCAAGACATAGCAGCTGCAAAAGGGAAGACTACAGCTCAGGTTGCACTCAGGTGGGTGTACCAAATAGGATCAAGTGCAATGGCAAAAAGCTTTAACAAGGAAAGGATGACACAAAACCTTGAAATATTTGATTTTGAGTTGAGTGAAGATGACTTAGAGAAGATTAAGCAGATTCCACAGCGAAGGCAATACTTAGGGGACATGTGGCTATCTGAAAATGGATCATGCAAGACCTTAGAAGAGCTTTGGGATGGAGATGTTTGA

Protein sequence

>Msa0961230

MAGNKIPDVLLNSGHKMPVIGMGTSVDNRPSNDVLASIFVDAIKVGYRHFDSASVYGTEEAIGMAVAKALEQGLIKSRDELFITSKPWNTDADYDLIVPALKTTLKKLGTEYVDLYLIHWPVRLRHDLENPVIFTKEDLLPFDIEGTWKAMEECYKLGLAKSIGICNYGTKKLTKLLETATITPAVNQVEMNPSWQQGKLREFCKEKGIHVSAWSALGAYKVTWGSGAVVENQILQDIAAAKGKTTAQVALRWVYQIGSSAMAKSFNKERMTQNLEIFDFELSEDDLEKIKQIPQRRQYLGDMWLSENGSCKTLEELWDGDV*