AlfalfaGEDB | Msa1246370

M. sativa cultivar zhongmu-4 / Msa1246370

KEGG: K10418
GO: -
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1246370	XP_013445572.1	99.083	109	1	0	1	109	1	109	2.61e-73	223

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1246370	sp\|O96860\|DYL2_DROME	55.556	90	38	1	1	90	1	88	2.05e-29	104

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1246370	A0A072TRW8	99.083	109	1	0	1	109	1	109	1.25e-73	223

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0079250	Msa1246370	0.810941	8.972203e-51	-8.615850e-47
Msa0607290	Msa1246370	-0.822104	2.831557e-53	-8.615850e-47
Msa0650620	Msa1246370	0.802485	5.486083e-49	-8.615850e-47
Msa0650650	Msa1246370	0.815785	7.739124e-52	-8.615850e-47
Msa0650740	Msa1246370	0.821704	3.505164e-53	-8.615850e-47
Msa0692420	Msa1246370	0.804963	1.678291e-49	-8.615850e-47
Msa0738850	Msa1246370	0.816092	6.607034e-52	-8.615850e-47
Msa0738860	Msa1246370	0.824411	8.188523e-54	-8.615850e-47
Msa0738870	Msa1246370	0.819422	1.171140e-52	-8.615850e-47
Msa0738880	Msa1246370	0.810161	1.322492e-50	-8.615850e-47
Msa0783260	Msa1246370	0.807365	5.239290e-50	-8.615850e-47
Msa0783270	Msa1246370	0.814314	1.640758e-51	-8.615850e-47
Msa0819320	Msa1246370	0.803984	2.684688e-49	-8.615850e-47
Msa0819360	Msa1246370	0.802341	5.874042e-49	-8.615850e-47
Msa0819370	Msa1246370	0.811452	6.951310e-51	-8.615850e-47
Msa0132620	Msa1246370	0.802266	6.087354e-49	-8.615850e-47
Msa0143080	Msa1246370	0.824387	8.297994e-54	-8.615850e-47
Msa0170560	Msa1246370	-0.814495	1.496531e-51	-8.615850e-47
Msa0185390	Msa1246370	0.826259	2.991875e-54	-8.615850e-47
Msa0194970	Msa1246370	0.814464	1.520201e-51	-8.615850e-47
Msa0223100	Msa1246370	0.803537	3.325420e-49	-8.615850e-47
Msa0236980	Msa1246370	0.819659	1.034250e-52	-8.615850e-47
Msa1246370	Msa1286680	0.835732	1.414072e-56	-8.615850e-47
Msa1246370	Msa1287360	0.885396	8.433540e-72	-8.615850e-47
Msa1246370	Msa1303160	0.806348	8.592008e-50	-8.615850e-47
Msa1246370	Msa1376640	0.801200	1.007291e-48	-8.615850e-47
Msa1246370	Msa1381130	0.826262	2.987667e-54	-8.615850e-47
Msa1246370	Msa1405770	0.809995	1.436462e-50	-8.615850e-47
Msa1246370	Msa1412080	0.812255	4.647067e-51	-8.615850e-47
Msa1246370	Msa1432640	0.809935	1.479219e-50	-8.615850e-47
Msa1246370	Msa1439230	0.800061	1.719422e-48	-8.615850e-47
Msa1246370	Msa1456650	0.832613	8.553150e-56	-8.615850e-47
Msa0371070	Msa1246370	0.819176	1.332665e-52	-8.615850e-47
Msa0467800	Msa1246370	0.816339	5.819998e-52	-8.615850e-47
Msa0467880	Msa1246370	0.807728	4.385724e-50	-8.615850e-47
Msa0482680	Msa1246370	0.807449	5.026404e-50	-8.615850e-47
Msa1015060	Msa1246370	0.824375	8.351250e-54	-8.615850e-47
Msa1069660	Msa1246370	-0.802795	4.736277e-49	-8.615850e-47
Msa1092820	Msa1246370	0.823711	1.195402e-53	-8.615850e-47
Msa1099690	Msa1246370	0.809347	1.979185e-50	-8.615850e-47
Msa0252180	Msa1246370	0.800537	1.376099e-48	-8.615850e-47
Msa0302650	Msa1246370	0.810349	1.204558e-50	-8.615850e-47
Msa0328210	Msa1246370	0.841468	4.666596e-58	-8.615850e-47
Msa1126120	Msa1246370	-0.814481	1.507334e-51	-8.615850e-47
Msa1218650	Msa1246370	0.810672	1.026056e-50	-8.615850e-47
Msa0532840	Msa1246370	0.806102	9.683411e-50	-8.615850e-47
Msa0533010	Msa1246370	0.804974	1.669789e-49	-8.615850e-47
Msa0579340	Msa1246370	0.834307	3.233073e-56	-8.615850e-47
Msa0583790	Msa1246370	0.818238	2.175347e-52	-8.615850e-47
Msa0859520	Msa1246370	0.818008	2.452472e-52	-8.615850e-47
Msa0859530	Msa1246370	0.817918	2.570573e-52	-8.615850e-47
Msa0942730	Msa1246370	0.801031	1.090588e-48	-8.615850e-47
Msa0950620	Msa1246370	0.802679	5.002872e-49	-8.615850e-47
Msa0950900	Msa1246370	0.802869	4.571019e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1246370	MtrunA17_Chr8g0360861	99.083	109	1	0	1	109	1	109	2.40e-77	223
Msa1246370	MtrunA17_Chr6g0485891	50.538	93	46	0	1	93	35	127	5.62e-30	103
Msa1246370	MtrunA17_Chr2g0331651	55.556	90	40	0	1	90	1	90	9.84e-30	102
Msa1246370	MtrunA17_Chr4g0067531	48.837	86	42	1	5	90	46	129	2.39e-26	94.7
Msa1246370	MtrunA17_Chr4g0063491	49.367	79	40	0	11	89	159	237	4.00e-22	87.0
Msa1246370	MtrunA17_Chr8g0391451	45.882	85	44	1	12	94	60	144	1.16e-19	78.2
Msa1246370	MtrunA17_Chr4g0059791	50.633	79	38	1	12	89	222	300	1.37e-18	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1246370	AT4G27360.1	71.845	103	29	0	1	103	1	103	1.71e-50	155
Msa1246370	AT3G16120.2	55.435	92	41	0	1	92	1	92	4.93e-33	110
Msa1246370	AT3G16120.1	55.435	92	41	0	1	92	1	92	4.93e-33	110
Msa1246370	AT1G52245.1	51.064	94	46	0	1	94	1	94	3.30e-30	103
Msa1246370	AT4G15930.1	49.412	85	41	1	5	89	39	121	3.59e-24	89.4
Msa1246370	AT5G20110.1	46.988	83	44	0	12	94	123	205	2.79e-21	84.3
Msa1246370	AT1G23220.1	39.773	88	52	1	8	94	38	125	4.12e-18	74.3
Msa1246370	AT4G15930.2	49.180	61	29	1	5	65	39	97	1.34e-13	61.6

Find 26 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCATTGTAGGCACTGATTT+TGG	0.221609	8_2:+37509428	Msa1246370:CDS
GCACAAGAGTAGAACAAAAT+AGG	0.295751	8_2:-37509084	None:intergenic
CAGGAATTTGATAGGGTTCA+TGG	0.298316	8_2:+37509392	Msa1246370:intron
CAGTTACATCATAGAAATCT+AGG	0.300776	8_2:-37509247	None:intergenic
AATTATTGCAGGAATTTGAT+AGG	0.304941	8_2:+37509384	Msa1246370:intron
ATCATAGAAATCTAGGGCTT+TGG	0.353606	8_2:-37509240	None:intergenic
CTTCAATTCATCATGCTAGA+AGG	0.354098	8_2:+37509152	Msa1246370:exon
TTGCTCTTTAAGGGATCTGC+TGG	0.420352	8_2:+37509512	Msa1246370:CDS
CCTGGTTGGCAATGCATTGT+AGG	0.440795	8_2:+37509416	Msa1246370:CDS
CTAGAAGGCAAGGCAGTGAT+TGG	0.481296	8_2:+37509167	Msa1246370:CDS
TAGGTTAAAGATAAAGTGAT+GGG	0.502859	8_2:-37509033	None:intergenic
CCTACAATGCATTGCCAACC+AGG	0.504627	8_2:-37509416	None:intergenic
AAATCAGTTCTCAGCAATAG+AGG	0.512873	8_2:+37509550	Msa1246370:CDS
TATAGCTCGATTTATAAAGA+AGG	0.514762	8_2:+37509280	Msa1246370:CDS
TTTGATAGGGTTCATGGACC+TGG	0.522418	8_2:+37509398	Msa1246370:CDS
ATTATTGCAGGAATTTGATA+GGG	0.524356	8_2:+37509385	Msa1246370:intron
TATAAATCGAGCTATATCAA+TGG	0.561045	8_2:-37509273	None:intergenic
GTAGGTTAAAGATAAAGTGA+TGG	0.580576	8_2:-37509034	None:intergenic
ATTCATCATGCTAGAAGGCA+AGG	0.581622	8_2:+37509157	Msa1246370:exon
TCCTTTGTGACACATTGTAG+TGG	0.583602	8_2:+37509452	Msa1246370:CDS
AGTTACATCATAGAAATCTA+GGG	0.585718	8_2:-37509246	None:intergenic
ATAGGGTTCATGGACCTGGT+TGG	0.594187	8_2:+37509402	Msa1246370:CDS
GGTTAAAGATAAAGTGATGG+GGG	0.603810	8_2:-37509031	None:intergenic
GCCACTACAATGTGTCACAA+AGG	0.659746	8_2:-37509453	None:intergenic
AGGTTAAAGATAAAGTGATG+GGG	0.668428	8_2:-37509032	None:intergenic
AGATTTCTATGATGTAACTG+AGG	0.680263	8_2:+37509250	Msa1246370:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGATTTGAAAAACAAAAAA+TGG	+	chr8_2:37509663-37509682	Msa1246370:three_prime_UTR	15.0%
!!!	ATAGGCTACTTTTTTTTTAT+TGG	-	chr8_2:37509069-37509088	None:intergenic	20.0%
!!	GGTAATAAATTAAAACTAAG+AGG	+	chr8_2:37509301-37509320	Msa1246370:intron	20.0%
!	TAGGTTAAAGATAAAGTGAT+GGG	-	chr8_2:37509036-37509055	None:intergenic	25.0%
!!!	TTTTATTGGTTGTAGTATGT+AGG	-	chr8_2:37509055-37509074	None:intergenic	25.0%
!	AGTTACATCATAGAAATCTA+GGG	-	chr8_2:37509249-37509268	None:intergenic	25.0%
!	TATAAATCGAGCTATATCAA+TGG	-	chr8_2:37509276-37509295	None:intergenic	25.0%
!	TATAGCTCGATTTATAAAGA+AGG	+	chr8_2:37509280-37509299	Msa1246370:CDS	25.0%
!	AAACTAAGAGGATTGTTTAT+TGG	+	chr8_2:37509313-37509332	Msa1246370:intron	25.0%
!	ATGTGTTAGTTAATTATTGC+AGG	+	chr8_2:37509373-37509392	Msa1246370:intron	25.0%
!	AATTATTGCAGGAATTTGAT+AGG	+	chr8_2:37509384-37509403	Msa1246370:intron	25.0%
!	ATTATTGCAGGAATTTGATA+GGG	+	chr8_2:37509385-37509404	Msa1246370:intron	25.0%
!!!	TGCTTCATCTATTTTTGTAT+AGG	+	chr8_2:37509476-37509495	Msa1246370:CDS	25.0%
	AGGTTAAAGATAAAGTGATG+GGG	-	chr8_2:37509035-37509054	None:intergenic	30.0%
	GTAGGTTAAAGATAAAGTGA+TGG	-	chr8_2:37509037-37509056	None:intergenic	30.0%
	CAGTTACATCATAGAAATCT+AGG	-	chr8_2:37509250-37509269	None:intergenic	30.0%
	AGATTTCTATGATGTAACTG+AGG	+	chr8_2:37509250-37509269	Msa1246370:CDS	30.0%
!!!	CTATTTTTGTATAGGTAGCA+TGG	+	chr8_2:37509484-37509503	Msa1246370:CDS	30.0%
!	ATGGCAATTTTGCTCTTTAA+GGG	+	chr8_2:37509503-37509522	Msa1246370:CDS	30.0%
	TGAAAAACAAAAAATGGCAG+AGG	+	chr8_2:37509669-37509688	Msa1246370:three_prime_UTR	30.0%
	GGTTAAAGATAAAGTGATGG+GGG	-	chr8_2:37509034-37509053	None:intergenic	35.0%
	GCACAAGAGTAGAACAAAAT+AGG	-	chr8_2:37509087-37509106	None:intergenic	35.0%
	CTTCAATTCATCATGCTAGA+AGG	+	chr8_2:37509152-37509171	Msa1246370:exon	35.0%
!	ATCATAGAAATCTAGGGCTT+TGG	-	chr8_2:37509243-37509262	None:intergenic	35.0%
!	CATGGCAATTTTGCTCTTTA+AGG	+	chr8_2:37509502-37509521	Msa1246370:CDS	35.0%
!!	AACTGATTTTCTTCTGCTTG+TGG	-	chr8_2:37509539-37509558	None:intergenic	35.0%
	AAATCAGTTCTCAGCAATAG+AGG	+	chr8_2:37509550-37509569	Msa1246370:CDS	35.0%
	ATTCATCATGCTAGAAGGCA+AGG	+	chr8_2:37509157-37509176	Msa1246370:exon	40.0%
	CAGGAATTTGATAGGGTTCA+TGG	+	chr8_2:37509392-37509411	Msa1246370:intron	40.0%
!!!	TGCATTGTAGGCACTGATTT+TGG	+	chr8_2:37509428-37509447	Msa1246370:CDS	40.0%
	TCCTTTGTGACACATTGTAG+TGG	+	chr8_2:37509452-37509471	Msa1246370:CDS	40.0%
!	GATAAAGTGATGGGGGATGA+TGG	-	chr8_2:37509027-37509046	None:intergenic	45.0%
	TTTGATAGGGTTCATGGACC+TGG	+	chr8_2:37509398-37509417	Msa1246370:CDS	45.0%
	GCCACTACAATGTGTCACAA+AGG	-	chr8_2:37509456-37509475	None:intergenic	45.0%
!	TTGCTCTTTAAGGGATCTGC+TGG	+	chr8_2:37509512-37509531	Msa1246370:CDS	45.0%
!!!	TTTTAATGTAATTTTTTTTA+TGG	+	chr8_2:37509339-37509358	Msa1246370:intron	5.0%
!	AAAGTGATGGGGGATGATGG+TGG	-	chr8_2:37509024-37509043	None:intergenic	50.0%
	CTAGAAGGCAAGGCAGTGAT+TGG	+	chr8_2:37509167-37509186	Msa1246370:CDS	50.0%
	ATAGGGTTCATGGACCTGGT+TGG	+	chr8_2:37509402-37509421	Msa1246370:CDS	50.0%
	CCTACAATGCATTGCCAACC+AGG	-	chr8_2:37509419-37509438	None:intergenic	50.0%
	CCTGGTTGGCAATGCATTGT+AGG	+	chr8_2:37509416-37509435	Msa1246370:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	37508994	37509702	37508994	ID=Msa1246370;Name=Msa1246370
chr8_2	mRNA	37508994	37509702	37508994	ID=Msa1246370-mRNA-1;Parent=Msa1246370;Name=Msa1246370-mRNA-1;_AED=0.07;_eAED=0.07;_QI=170\|1\|1\|1\|1\|1\|2\|116\|109
chr8_2	exon	37508994	37509301	37508994	ID=Msa1246370-mRNA-1:exon:11812;Parent=Msa1246370-mRNA-1
chr8_2	exon	37509395	37509702	37509395	ID=Msa1246370-mRNA-1:exon:11813;Parent=Msa1246370-mRNA-1
chr8_2	five_prime_UTR	37508994	37509163	37508994	ID=Msa1246370-mRNA-1:five_prime_utr;Parent=Msa1246370-mRNA-1
chr8_2	CDS	37509164	37509301	37509164	ID=Msa1246370-mRNA-1:cds;Parent=Msa1246370-mRNA-1
chr8_2	CDS	37509395	37509586	37509395	ID=Msa1246370-mRNA-1:cds;Parent=Msa1246370-mRNA-1
chr8_2	three_prime_UTR	37509587	37509702	37509587	ID=Msa1246370-mRNA-1:three_prime_utr;Parent=Msa1246370-mRNA-1

Gene Sequence	>Msa1246370 ATGCTAGAAGGCAAGGCAGTGATTGGTGAGACTGATATGCTTCAGACTATGCAACAAGATGCTCTTGATTTAGCATCCAAAGCCCTAGATTTCTATGATGTAACTGAGGCCATTGATATAGCTCGATTTATAAAGAAGGAATTTGATAGGGTTCATGGACCTGGTTGGCAATGCATTGTAGGCACTGATTTTGGTTCCTTTGTGACACATTGTAGTGGCTGCTTCATCTATTTTTGTATAGGTAGCATGGCAATTTTGCTCTTTAAGGGATCTGCTGGTCCACAAGCAGAAGAAAATCAGTTCTCAGCAATAGAGGCAACTAAAGCATAA
Protein sequence	>Msa1246370 MLEGKAVIGETDMLQTMQQDALDLASKALDFYDVTEAIDIARFIKKEFDRVHGPGWQCIVGTDFGSFVTHCSGCFIYFCIGSMAILLFKGSAGPQAEENQFSAIEATKA*