Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607290 | PNX94518.1 | 96.053 | 152 | 6 | 0 | 1 | 152 | 235 | 386 | 2.58e-101 | 306 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607290 | sp|O81742|APBLC_ARATH | 68.421 | 152 | 48 | 0 | 1 | 152 | 742 | 893 | 7.77e-71 | 231 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607290 | A0A2K3MUM1 | 96.053 | 152 | 6 | 0 | 1 | 152 | 235 | 386 | 1.23e-101 | 306 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000490 | Msa0607290 | 0.850230 | 1.941716e-60 | -8.615850e-47 |
| Msa0001950 | Msa0607290 | -0.827386 | 1.609667e-54 | -8.615850e-47 |
| Msa0011180 | Msa0607290 | -0.803622 | 3.193273e-49 | -8.615850e-47 |
| Msa0029540 | Msa0607290 | 0.808666 | 2.768921e-50 | -8.615850e-47 |
| Msa0030310 | Msa0607290 | -0.806568 | 7.721653e-50 | -8.615850e-47 |
| Msa0032370 | Msa0607290 | -0.809983 | 1.444578e-50 | -8.615850e-47 |
| Msa0050730 | Msa0607290 | -0.807610 | 4.646789e-50 | -8.615850e-47 |
| Msa0072170 | Msa0607290 | -0.817862 | 2.645425e-52 | -8.615850e-47 |
| Msa0078000 | Msa0607290 | 0.803428 | 3.503092e-49 | -8.615850e-47 |
| Msa0079250 | Msa0607290 | -0.821477 | 3.954226e-53 | -8.615850e-47 |
| Msa0095380 | Msa0607290 | -0.834967 | 2.206887e-56 | -8.615850e-47 |
| Msa0120540 | Msa0607290 | -0.817302 | 3.538602e-52 | -8.615850e-47 |
| Msa0607290 | Msa0609970 | -0.833777 | 4.388880e-56 | -8.615850e-47 |
| Msa0607290 | Msa0614210 | -0.820366 | 7.124228e-53 | -8.615850e-47 |
| Msa0607290 | Msa0618010 | -0.803631 | 3.179456e-49 | -8.615850e-47 |
| Msa0607290 | Msa0625790 | -0.819302 | 1.247360e-52 | -8.615850e-47 |
| Msa0607290 | Msa0638690 | -0.821915 | 3.131551e-53 | -8.615850e-47 |
| Msa0607290 | Msa0647450 | 0.800424 | 1.450747e-48 | -8.615850e-47 |
| Msa0607290 | Msa0650190 | -0.838164 | 3.384313e-57 | -8.615850e-47 |
| Msa0607290 | Msa0650620 | -0.833439 | 5.328890e-56 | -8.615850e-47 |
| Msa0607290 | Msa0669200 | -0.807526 | 4.840863e-50 | -8.615850e-47 |
| Msa0607290 | Msa0669850 | -0.840219 | 9.918380e-58 | -8.615850e-47 |
| Msa0607290 | Msa0670980 | -0.811610 | 6.422230e-51 | -8.615850e-47 |
| Msa0607290 | Msa0690560 | -0.805238 | 1.470215e-49 | -8.615850e-47 |
| Msa0607290 | Msa0692110 | -0.831598 | 1.524459e-55 | -8.615850e-47 |
| Msa0607290 | Msa0694250 | -0.802136 | 6.473622e-49 | -8.615850e-47 |
| Msa0607290 | Msa0697400 | -0.829852 | 4.081205e-55 | -8.615850e-47 |
| Msa0607290 | Msa0703590 | -0.804073 | 2.573795e-49 | -8.615850e-47 |
| Msa0607290 | Msa0709190 | -0.804647 | 1.953736e-49 | -8.615850e-47 |
| Msa0607290 | Msa0711490 | -0.818860 | 1.572409e-52 | -8.615850e-47 |
| Msa0607290 | Msa0712310 | -0.824371 | 8.371094e-54 | -8.615850e-47 |
| Msa0607290 | Msa0725920 | -0.815954 | 7.093128e-52 | -8.615850e-47 |
| Msa0607290 | Msa0738850 | -0.821155 | 4.691947e-53 | -8.615850e-47 |
| Msa0607290 | Msa0752620 | -0.819664 | 1.031172e-52 | -8.615850e-47 |
| Msa0607290 | Msa0756310 | -0.801939 | 7.106089e-49 | -8.615850e-47 |
| Msa0607290 | Msa0766470 | -0.816918 | 4.316892e-52 | -8.615850e-47 |
| Msa0607290 | Msa0775260 | -0.838933 | 2.141979e-57 | -8.615850e-47 |
| Msa0607290 | Msa0791910 | -0.821522 | 3.860445e-53 | -8.615850e-47 |
| Msa0607290 | Msa0792090 | -0.827133 | 1.850877e-54 | -8.615850e-47 |
| Msa0607290 | Msa0793720 | -0.801409 | 9.128684e-49 | -8.615850e-47 |
| Msa0607290 | Msa0794320 | -0.803030 | 4.233354e-49 | -8.615850e-47 |
| Msa0607290 | Msa0794720 | -0.817024 | 4.087076e-52 | -8.615850e-47 |
| Msa0607290 | Msa0795010 | -0.820795 | 5.679906e-53 | -8.615850e-47 |
| Msa0607290 | Msa0795030 | -0.825293 | 5.072268e-54 | -8.615850e-47 |
| Msa0607290 | Msa0798810 | 0.807219 | 5.625649e-50 | -8.615850e-47 |
| Msa0607290 | Msa0820530 | 0.801273 | 9.733324e-49 | -8.615850e-47 |
| Msa0607290 | Msa0830990 | -0.824320 | 8.605286e-54 | -8.615850e-47 |
| Msa0607290 | Msa0835730 | -0.810040 | 1.404589e-50 | -8.615850e-47 |
| Msa0607290 | Msa0846030 | -0.811269 | 7.617089e-51 | -8.615850e-47 |
| Msa0607290 | Msa0852730 | -0.835975 | 1.226630e-56 | -8.615850e-47 |
| Msa0607290 | Msa0853170 | -0.813314 | 2.724639e-51 | -8.615850e-47 |
| Msa0607290 | Msa0861040 | 0.802450 | 5.577182e-49 | -8.615850e-47 |
| Msa0607290 | Msa0883460 | -0.808489 | 3.021134e-50 | -8.615850e-47 |
| Msa0607290 | Msa0891600 | 0.817954 | 2.521907e-52 | -8.615850e-47 |
| Msa0607290 | Msa0908170 | -0.810063 | 1.388374e-50 | -8.615850e-47 |
| Msa0607290 | Msa0920550 | -0.803511 | 3.366835e-49 | -8.615850e-47 |
| Msa0607290 | Msa0922540 | -0.807384 | 5.190444e-50 | -8.615850e-47 |
| Msa0607290 | Msa0942730 | -0.808400 | 3.155497e-50 | -8.615850e-47 |
| Msa0607290 | Msa0946080 | -0.807532 | 4.828845e-50 | -8.615850e-47 |
| Msa0607290 | Msa0957450 | 0.801041 | 1.085583e-48 | -8.615850e-47 |
| Msa0607290 | Msa0959010 | -0.822815 | 1.936174e-53 | -8.615850e-47 |
| Msa0607290 | Msa0993530 | -0.848904 | 4.551721e-60 | -8.615850e-47 |
| Msa0607290 | Msa1015060 | -0.836530 | 8.866155e-57 | -8.615850e-47 |
| Msa0607290 | Msa1026760 | -0.810029 | 1.412507e-50 | -8.615850e-47 |
| Msa0607290 | Msa1092820 | -0.854408 | 1.255676e-61 | -8.615850e-47 |
| Msa0607290 | Msa1098720 | 0.811298 | 7.508105e-51 | -8.615850e-47 |
| Msa0607290 | Msa1109570 | -0.813215 | 2.865210e-51 | -8.615850e-47 |
| Msa0607290 | Msa1126120 | 0.839927 | 1.182317e-57 | -8.615850e-47 |
| Msa0607290 | Msa1130210 | 0.816433 | 5.544570e-52 | -8.615850e-47 |
| Msa0607290 | Msa1132520 | -0.806370 | 8.502613e-50 | -8.615850e-47 |
| Msa0607290 | Msa1138900 | 0.801730 | 7.844428e-49 | -8.615850e-47 |
| Msa0607290 | Msa1171770 | 0.838485 | 2.796976e-57 | -8.615850e-47 |
| Msa0607290 | Msa1172130 | 0.804513 | 2.083381e-49 | -8.615850e-47 |
| Msa0607290 | Msa1175330 | -0.822986 | 1.766396e-53 | -8.615850e-47 |
| Msa0607290 | Msa1218650 | -0.800439 | 1.440499e-48 | -8.615850e-47 |
| Msa0607290 | Msa1219430 | -0.824245 | 8.959097e-54 | -8.615850e-47 |
| Msa0607290 | Msa1221900 | -0.801808 | 7.560195e-49 | -8.615850e-47 |
| Msa0607290 | Msa1235630 | -0.826792 | 2.233934e-54 | -8.615850e-47 |
| Msa0607290 | Msa1236070 | 0.857587 | 1.474698e-62 | -8.615850e-47 |
| Msa0607290 | Msa1237030 | -0.850818 | 1.328092e-60 | -8.615850e-47 |
| Msa0607290 | Msa1246370 | -0.822104 | 2.831557e-53 | -8.615850e-47 |
| Msa0607290 | Msa1287360 | -0.824809 | 6.599400e-54 | -8.615850e-47 |
| Msa0607290 | Msa1299360 | -0.803143 | 4.012030e-49 | -8.615850e-47 |
| Msa0607290 | Msa1300350 | 0.802103 | 6.576191e-49 | -8.615850e-47 |
| Msa0607290 | Msa1303160 | -0.810854 | 9.369461e-51 | -8.615850e-47 |
| Msa0607290 | Msa1315870 | -0.805062 | 1.600134e-49 | -8.615850e-47 |
| Msa0607290 | Msa1323060 | -0.800100 | 1.688510e-48 | -8.615850e-47 |
| Msa0607290 | Msa1327760 | -0.812795 | 3.541695e-51 | -8.615850e-47 |
| Msa0607290 | Msa1350690 | -0.807793 | 4.248918e-50 | -8.615850e-47 |
| Msa0607290 | Msa1362510 | -0.825662 | 4.147235e-54 | -8.615850e-47 |
| Msa0607290 | Msa1373510 | -0.837351 | 5.471459e-57 | -8.615850e-47 |
| Msa0607290 | Msa1382310 | -0.837625 | 4.655941e-57 | -8.615850e-47 |
| Msa0607290 | Msa1382410 | -0.816408 | 5.616717e-52 | -8.615850e-47 |
| Msa0607290 | Msa1389780 | -0.812046 | 5.163558e-51 | -8.615850e-47 |
| Msa0607290 | Msa1390310 | -0.819561 | 1.088523e-52 | -8.615850e-47 |
| Msa0607290 | Msa1393800 | -0.818607 | 1.794880e-52 | -8.615850e-47 |
| Msa0607290 | Msa1412080 | -0.825132 | 5.537358e-54 | -8.615850e-47 |
| Msa0607290 | Msa1412140 | -0.806057 | 9.897114e-50 | -8.615850e-47 |
| Msa0607290 | Msa1432640 | -0.838668 | 2.507502e-57 | -8.615850e-47 |
| Msa0607290 | Msa1438450 | -0.820410 | 6.960056e-53 | -8.615850e-47 |
| Msa0607290 | Msa1439230 | -0.801265 | 9.768050e-49 | -8.615850e-47 |
| Msa0607290 | Msa1439960 | -0.810187 | 1.305558e-50 | -8.615850e-47 |
| Msa0607290 | Msa1444450 | -0.827413 | 1.586334e-54 | -8.615850e-47 |
| Msa0607290 | Msa1456650 | -0.828611 | 8.167380e-55 | -8.615850e-47 |
| Msa0132620 | Msa0607290 | -0.800436 | 1.442884e-48 | -8.615850e-47 |
| Msa0138970 | Msa0607290 | 0.822482 | 2.313614e-53 | -8.615850e-47 |
| Msa0140520 | Msa0607290 | 0.809269 | 2.056796e-50 | -8.615850e-47 |
| Msa0143080 | Msa0607290 | -0.870244 | 1.689229e-66 | -8.615850e-47 |
| Msa0145950 | Msa0607290 | -0.809657 | 1.697944e-50 | -8.615850e-47 |
| Msa0168640 | Msa0607290 | 0.809729 | 1.638674e-50 | -8.615850e-47 |
| Msa0170560 | Msa0607290 | 0.819832 | 9.440553e-53 | -8.615850e-47 |
| Msa0172350 | Msa0607290 | -0.819833 | 9.434298e-53 | -8.615850e-47 |
| Msa0194970 | Msa0607290 | -0.863290 | 2.767077e-64 | -8.615850e-47 |
| Msa0212750 | Msa0607290 | 0.833191 | 6.144275e-56 | -8.615850e-47 |
| Msa0223290 | Msa0607290 | -0.811115 | 8.225059e-51 | -8.615850e-47 |
| Msa0224730 | Msa0607290 | -0.821187 | 4.612778e-53 | -8.615850e-47 |
| Msa0227310 | Msa0607290 | 0.801966 | 7.016881e-49 | -8.615850e-47 |
| Msa0236980 | Msa0607290 | -0.875056 | 4.160090e-68 | -8.615850e-47 |
| Msa0374250 | Msa0607290 | -0.801252 | 9.827726e-49 | -8.615850e-47 |
| Msa0377530 | Msa0607290 | -0.811270 | 7.612770e-51 | -8.615850e-47 |
| Msa0392070 | Msa0607290 | -0.814953 | 1.184745e-51 | -8.615850e-47 |
| Msa0403190 | Msa0607290 | -0.800743 | 1.249185e-48 | -8.615850e-47 |
| Msa0419640 | Msa0607290 | 0.833282 | 5.832883e-56 | -8.615850e-47 |
| Msa0426810 | Msa0607290 | -0.809686 | 1.673777e-50 | -8.615850e-47 |
| Msa0441800 | Msa0607290 | -0.805692 | 1.180486e-49 | -8.615850e-47 |
| Msa0449820 | Msa0607290 | -0.811876 | 5.621647e-51 | -8.615850e-47 |
| Msa0469910 | Msa0607290 | 0.824558 | 7.564784e-54 | -8.615850e-47 |
| Msa0482680 | Msa0607290 | -0.827573 | 1.451939e-54 | -8.615850e-47 |
| Msa0259330 | Msa0607290 | -0.802333 | 5.895766e-49 | -8.615850e-47 |
| Msa0266210 | Msa0607290 | -0.812476 | 4.160096e-51 | -8.615850e-47 |
| Msa0267760 | Msa0607290 | -0.811644 | 6.313816e-51 | -8.615850e-47 |
| Msa0275030 | Msa0607290 | -0.821140 | 4.729439e-53 | -8.615850e-47 |
| Msa0277530 | Msa0607290 | -0.812413 | 4.293806e-51 | -8.615850e-47 |
| Msa0287670 | Msa0607290 | -0.809067 | 2.272827e-50 | -8.615850e-47 |
| Msa0302650 | Msa0607290 | -0.810397 | 1.176189e-50 | -8.615850e-47 |
| Msa0305640 | Msa0607290 | -0.811563 | 6.577935e-51 | -8.615850e-47 |
| Msa0328210 | Msa0607290 | -0.875253 | 3.564675e-68 | -8.615850e-47 |
| Msa0505060 | Msa0607290 | -0.829579 | 4.756972e-55 | -8.615850e-47 |
| Msa0517490 | Msa0607290 | -0.809796 | 1.584704e-50 | -8.615850e-47 |
| Msa0521310 | Msa0607290 | -0.807074 | 6.037289e-50 | -8.615850e-47 |
| Msa0526710 | Msa0607290 | -0.806474 | 8.081846e-50 | -8.615850e-47 |
| Msa0527590 | Msa0607290 | -0.806404 | 8.364023e-50 | -8.615850e-47 |
| Msa0535030 | Msa0607290 | -0.814947 | 1.188355e-51 | -8.615850e-47 |
| Msa0535060 | Msa0607290 | -0.815674 | 8.191240e-52 | -8.615850e-47 |
| Msa0554180 | Msa0607290 | 0.831391 | 1.713864e-55 | -8.615850e-47 |
| Msa0579370 | Msa0607290 | -0.801586 | 8.396517e-49 | -8.615850e-47 |
| Msa0590660 | Msa0607290 | -0.803597 | 3.231438e-49 | -8.615850e-47 |
| Msa0591060 | Msa0607290 | -0.848324 | 6.592697e-60 | -8.615850e-47 |
| Msa0591770 | Msa0607290 | -0.835953 | 1.242536e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607290 | MtrunA17_Chr4g0038901 | 98.684 | 152 | 2 | 0 | 1 | 152 | 745 | 896 | 6.91e-103 | 313 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607290 | AT4G23460.2 | 68.421 | 152 | 48 | 0 | 1 | 152 | 648 | 799 | 3.86e-72 | 231 |
| Msa0607290 | AT4G23460.1 | 68.421 | 152 | 48 | 0 | 1 | 152 | 742 | 893 | 7.90e-72 | 231 |
| Msa0607290 | AT4G11380.1 | 68.421 | 152 | 48 | 0 | 1 | 152 | 743 | 894 | 1.77e-71 | 230 |
| Msa0607290 | AT4G11380.2 | 68.421 | 152 | 48 | 0 | 1 | 152 | 765 | 916 | 1.83e-71 | 231 |
Find 50 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAAATGTTGACATTTC+AGG | 0.156965 | 4_2:-54086465 | None:intergenic |
| TTCAAACTTAAACATCAATT+TGG | 0.212344 | 4_2:-54086603 | None:intergenic |
| GACTTCCCTGCAATTGTAAT+TGG | 0.251072 | 4_2:+54086258 | Msa0607290:CDS |
| CTCACTGTTGCATATATAAC+TGG | 0.256477 | 4_2:+54086634 | Msa0607290:three_prime_UTR |
| GCGGACCTTGGGACATGTTC+TGG | 0.317144 | 4_2:-54085840 | None:intergenic |
| AAGCCATCGAGTCTCTTCTT+AGG | 0.334967 | 4_2:+54086490 | Msa0607290:CDS |
| TTGGAAACTTCATTTGAATC+AGG | 0.346160 | 4_2:-54086234 | None:intergenic |
| GACATCATTTGTTGAGAGAT+TGG | 0.368131 | 4_2:+54086664 | Msa0607290:three_prime_UTR |
| AATGTTGACATTTCAGGGCT+TGG | 0.368312 | 4_2:-54086459 | None:intergenic |
| ACACCACCAATTACAATTGC+AGG | 0.401904 | 4_2:-54086264 | None:intergenic |
| AGATAAATGTTGACATTTCA+GGG | 0.417984 | 4_2:-54086464 | None:intergenic |
| ATCGAACTTACCACAGTGGT+TGG | 0.451475 | 4_2:+54086405 | Msa0607290:CDS |
| TACAATTGCAGGGAAGTCCT+TGG | 0.454004 | 4_2:-54086253 | None:intergenic |
| AACTGAGCTAGGCGGACCTT+GGG | 0.475776 | 4_2:-54085851 | None:intergenic |
| TGACGCAACCGTGGAACGAC+TGG | 0.483279 | 4_2:+54086287 | Msa0607290:CDS |
| TTCCCTGCAATTGTAATTGG+TGG | 0.493912 | 4_2:+54086261 | Msa0607290:CDS |
| ACCACAGTGGTTGGAAACCC+TGG | 0.504670 | 4_2:+54086414 | Msa0607290:CDS |
| TCATTTGTTGAGAGATTGGA+TGG | 0.508720 | 4_2:+54086668 | Msa0607290:three_prime_UTR |
| AAACTGAGCTAGGCGGACCT+TGG | 0.509914 | 4_2:-54085852 | None:intergenic |
| GCAACCTGTAAAACTGAGCT+AGG | 0.514067 | 4_2:-54085862 | None:intergenic |
| AGCTAGGACACTCCTTCCTA+TGG | 0.514389 | 4_2:+54085810 | Msa0607290:exon |
| AAAGCGTAAGAATGCGAACC+AGG | 0.519843 | 4_2:+54086335 | Msa0607290:CDS |
| CTGAGAAGTAAAACACATCC+TGG | 0.525115 | 4_2:-54086353 | None:intergenic |
| AAACTTCATTTGAATCAGGA+AGG | 0.528035 | 4_2:-54086230 | None:intergenic |
| TGATTCAAATGAAGTTTCCA+AGG | 0.545833 | 4_2:+54086236 | Msa0607290:CDS |
| TGATGCGGCCAGTCGTTCCA+CGG | 0.546270 | 4_2:-54086295 | None:intergenic |
| TTGATTGCACATTTGACACC+AGG | 0.552977 | 4_2:-54086432 | None:intergenic |
| GTGGTAAGTTCGATTAAAAG+TGG | 0.560911 | 4_2:-54086396 | None:intergenic |
| CACCACCAATTACAATTGCA+GGG | 0.587784 | 4_2:-54086263 | None:intergenic |
| AACTTCATTTGAATCAGGAA+GGG | 0.589379 | 4_2:-54086229 | None:intergenic |
| ACGCTCCATTCTACCATCCT+CGG | 0.600658 | 4_2:-54085950 | None:intergenic |
| TGAAATACCACACAGGCTGT+TGG | 0.604545 | 4_2:-54085897 | None:intergenic |
| GGTATATACCTCAAGGAAAG+CGG | 0.620389 | 4_2:-54085974 | None:intergenic |
| GGAGCGTGCCGCTTTCCTTG+AGG | 0.622922 | 4_2:+54085966 | Msa0607290:CDS |
| AATAAAGAATATGTTTGATG+CGG | 0.626923 | 4_2:-54086310 | None:intergenic |
| ATGTTCTGGAACATAACCAT+AGG | 0.629968 | 4_2:-54085826 | None:intergenic |
| TCTCAGCTAAACTGCCTCGA+GGG | 0.631838 | 4_2:+54086370 | Msa0607290:CDS |
| ACTCCTAAGAAGAGACTCGA+TGG | 0.645293 | 4_2:-54086493 | None:intergenic |
| TGATTGCACATTTGACACCA+GGG | 0.646294 | 4_2:-54086431 | None:intergenic |
| TCTGGAACATAACCATAGGA+AGG | 0.648359 | 4_2:-54085822 | None:intergenic |
| AAAACAACCAACAGCCTGTG+TGG | 0.657599 | 4_2:+54085890 | Msa0607290:CDS |
| ATGTTCCAGAACATGTCCCA+AGG | 0.663630 | 4_2:+54085835 | Msa0607290:CDS |
| TGGTGGTGTTGACGCAACCG+TGG | 0.673768 | 4_2:+54086278 | Msa0607290:CDS |
| TTCTCAGCTAAACTGCCTCG+AGG | 0.693114 | 4_2:+54086369 | Msa0607290:CDS |
| TTTAATCGAACTTACCACAG+TGG | 0.700455 | 4_2:+54086401 | Msa0607290:CDS |
| ATTAAAAGTGGTATCCCTCG+AGG | 0.702719 | 4_2:-54086384 | None:intergenic |
| ACCTGTAAAACTGAGCTAGG+CGG | 0.713408 | 4_2:-54085859 | None:intergenic |
| TTATCGTTGAAATACCACAC+AGG | 0.723681 | 4_2:-54085904 | None:intergenic |
| ACCAGGGTTTCCAACCACTG+TGG | 0.743969 | 4_2:-54086415 | None:intergenic |
| TGAATGTGTTGCAGACATGG+AGG | 0.753034 | 4_2:+54086205 | Msa0607290:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATTTGTAATTTTTTTAA+TGG | + | chr4_2:54086062-54086081 | Msa0607290:intron | 10.0% |
| !!! | TGATTTGTAATTTTTTTAAT+GGG | + | chr4_2:54086063-54086082 | Msa0607290:intron | 10.0% |
| !!! | TTGATTTGTAATTTTTTTAA+TGG | + | chr4_2:54086062-54086081 | Msa0607290:intron | 10.0% |
| !!! | TGATTTGTAATTTTTTTAAT+GGG | + | chr4_2:54086063-54086082 | Msa0607290:intron | 10.0% |
| !!! | GGATTATTTCTATGTTATTA+AGG | + | chr4_2:54086084-54086103 | Msa0607290:intron | 20.0% |
| !! | AATAAAGAATATGTTTGATG+CGG | - | chr4_2:54086313-54086332 | None:intergenic | 20.0% |
| !!! | GTTTATACATTGTTTTTTGT+TGG | + | chr4_2:54086564-54086583 | Msa0607290:three_prime_UTR | 20.0% |
| !! | TTCAAACTTAAACATCAATT+TGG | - | chr4_2:54086606-54086625 | None:intergenic | 20.0% |
| !!! | GGATTATTTCTATGTTATTA+AGG | + | chr4_2:54086084-54086103 | Msa0607290:intron | 20.0% |
| !! | AATAAAGAATATGTTTGATG+CGG | - | chr4_2:54086313-54086332 | None:intergenic | 20.0% |
| !!! | GTTTATACATTGTTTTTTGT+TGG | + | chr4_2:54086564-54086583 | Msa0607290:three_prime_UTR | 20.0% |
| !! | TTCAAACTTAAACATCAATT+TGG | - | chr4_2:54086606-54086625 | None:intergenic | 20.0% |
| ! | TCTATGTTATTAAGGACTAA+AGG | + | chr4_2:54086092-54086111 | Msa0607290:intron | 25.0% |
| ! | AGATAAATGTTGACATTTCA+GGG | - | chr4_2:54086467-54086486 | None:intergenic | 25.0% |
| ! | AAGATAAATGTTGACATTTC+AGG | - | chr4_2:54086468-54086487 | None:intergenic | 25.0% |
| ! | TCTATGTTATTAAGGACTAA+AGG | + | chr4_2:54086092-54086111 | Msa0607290:intron | 25.0% |
| ! | AGATAAATGTTGACATTTCA+GGG | - | chr4_2:54086467-54086486 | None:intergenic | 25.0% |
| ! | AAGATAAATGTTGACATTTC+AGG | - | chr4_2:54086468-54086487 | None:intergenic | 25.0% |
| AAGAGATCAAATGAATGGAA+AGG | - | chr4_2:54085998-54086017 | None:intergenic | 30.0% | |
| GTGAAAAGAGATCAAATGAA+TGG | - | chr4_2:54086003-54086022 | None:intergenic | 30.0% | |
| TCACTGCAATAACATTAATG+CGG | + | chr4_2:54086019-54086038 | Msa0607290:intron | 30.0% | |
| CAAGTATAAGTCATGAAAAG+TGG | - | chr4_2:54086160-54086179 | None:intergenic | 30.0% | |
| ! | CACTTTTCATGACTTATACT+TGG | + | chr4_2:54086158-54086177 | Msa0607290:intron | 30.0% |
| AACTTCATTTGAATCAGGAA+GGG | - | chr4_2:54086232-54086251 | None:intergenic | 30.0% | |
| AAACTTCATTTGAATCAGGA+AGG | - | chr4_2:54086233-54086252 | None:intergenic | 30.0% | |
| TTGGAAACTTCATTTGAATC+AGG | - | chr4_2:54086237-54086256 | None:intergenic | 30.0% | |
| TGATTCAAATGAAGTTTCCA+AGG | + | chr4_2:54086236-54086255 | Msa0607290:CDS | 30.0% | |
| !!! | TGCATATATAACTGGTTTTG+TGG | + | chr4_2:54086642-54086661 | Msa0607290:three_prime_UTR | 30.0% |
| AAGAGATCAAATGAATGGAA+AGG | - | chr4_2:54085998-54086017 | None:intergenic | 30.0% | |
| GTGAAAAGAGATCAAATGAA+TGG | - | chr4_2:54086003-54086022 | None:intergenic | 30.0% | |
| TCACTGCAATAACATTAATG+CGG | + | chr4_2:54086019-54086038 | Msa0607290:intron | 30.0% | |
| CAAGTATAAGTCATGAAAAG+TGG | - | chr4_2:54086160-54086179 | None:intergenic | 30.0% | |
| ! | CACTTTTCATGACTTATACT+TGG | + | chr4_2:54086158-54086177 | Msa0607290:intron | 30.0% |
| AACTTCATTTGAATCAGGAA+GGG | - | chr4_2:54086232-54086251 | None:intergenic | 30.0% | |
| AAACTTCATTTGAATCAGGA+AGG | - | chr4_2:54086233-54086252 | None:intergenic | 30.0% | |
| TTGGAAACTTCATTTGAATC+AGG | - | chr4_2:54086237-54086256 | None:intergenic | 30.0% | |
| TGATTCAAATGAAGTTTCCA+AGG | + | chr4_2:54086236-54086255 | Msa0607290:CDS | 30.0% | |
| !!! | TGCATATATAACTGGTTTTG+TGG | + | chr4_2:54086642-54086661 | Msa0607290:three_prime_UTR | 30.0% |
| !! | ATGTTCTGGAACATAACCAT+AGG | - | chr4_2:54085829-54085848 | None:intergenic | 35.0% |
| !!! | AGGCTGTTGGTTGTTTTTTA+AGG | - | chr4_2:54085887-54085906 | None:intergenic | 35.0% |
| TTATCGTTGAAATACCACAC+AGG | - | chr4_2:54085907-54085926 | None:intergenic | 35.0% | |
| !!! | ATTCCAAGCATTTTTTACCG+AGG | + | chr4_2:54085933-54085952 | Msa0607290:CDS | 35.0% |
| ! | ATGGAAAGGTATATACCTCA+AGG | - | chr4_2:54085984-54086003 | None:intergenic | 35.0% |
| CTGCAACACATTCAATGATA+AGG | - | chr4_2:54086199-54086218 | None:intergenic | 35.0% | |
| GTGGTAAGTTCGATTAAAAG+TGG | - | chr4_2:54086399-54086418 | None:intergenic | 35.0% | |
| TTTAATCGAACTTACCACAG+TGG | + | chr4_2:54086401-54086420 | Msa0607290:CDS | 35.0% | |
| CTCACTGTTGCATATATAAC+TGG | + | chr4_2:54086634-54086653 | Msa0607290:three_prime_UTR | 35.0% | |
| ! | GACATCATTTGTTGAGAGAT+TGG | + | chr4_2:54086664-54086683 | Msa0607290:three_prime_UTR | 35.0% |
| ! | TCATTTGTTGAGAGATTGGA+TGG | + | chr4_2:54086668-54086687 | Msa0607290:three_prime_UTR | 35.0% |
| !! | ATGTTCTGGAACATAACCAT+AGG | - | chr4_2:54085829-54085848 | None:intergenic | 35.0% |
| !!! | AGGCTGTTGGTTGTTTTTTA+AGG | - | chr4_2:54085887-54085906 | None:intergenic | 35.0% |
| TTATCGTTGAAATACCACAC+AGG | - | chr4_2:54085907-54085926 | None:intergenic | 35.0% | |
| !!! | ATTCCAAGCATTTTTTACCG+AGG | + | chr4_2:54085933-54085952 | Msa0607290:CDS | 35.0% |
| ! | ATGGAAAGGTATATACCTCA+AGG | - | chr4_2:54085984-54086003 | None:intergenic | 35.0% |
| CTGCAACACATTCAATGATA+AGG | - | chr4_2:54086199-54086218 | None:intergenic | 35.0% | |
| GTGGTAAGTTCGATTAAAAG+TGG | - | chr4_2:54086399-54086418 | None:intergenic | 35.0% | |
| TTTAATCGAACTTACCACAG+TGG | + | chr4_2:54086401-54086420 | Msa0607290:CDS | 35.0% | |
| CTCACTGTTGCATATATAAC+TGG | + | chr4_2:54086634-54086653 | Msa0607290:three_prime_UTR | 35.0% | |
| ! | GACATCATTTGTTGAGAGAT+TGG | + | chr4_2:54086664-54086683 | Msa0607290:three_prime_UTR | 35.0% |
| ! | TCATTTGTTGAGAGATTGGA+TGG | + | chr4_2:54086668-54086687 | Msa0607290:three_prime_UTR | 35.0% |
| ! | TCTGGAACATAACCATAGGA+AGG | - | chr4_2:54085825-54085844 | None:intergenic | 40.0% |
| CATCCTCGGTAAAAAATGCT+TGG | - | chr4_2:54085939-54085958 | None:intergenic | 40.0% | |
| !!! | CAAGCATTTTTTACCGAGGA+TGG | + | chr4_2:54085937-54085956 | Msa0607290:CDS | 40.0% |
| ! | TTTTACCGAGGATGGTAGAA+TGG | + | chr4_2:54085945-54085964 | Msa0607290:CDS | 40.0% |
| GGTATATACCTCAAGGAAAG+CGG | - | chr4_2:54085977-54085996 | None:intergenic | 40.0% | |
| CATTGAATGTGTTGCAGACA+TGG | + | chr4_2:54086202-54086221 | Msa0607290:intron | 40.0% | |
| GACTTCCCTGCAATTGTAAT+TGG | + | chr4_2:54086258-54086277 | Msa0607290:CDS | 40.0% | |
| TTCCCTGCAATTGTAATTGG+TGG | + | chr4_2:54086261-54086280 | Msa0607290:CDS | 40.0% | |
| CACCACCAATTACAATTGCA+GGG | - | chr4_2:54086266-54086285 | None:intergenic | 40.0% | |
| ACACCACCAATTACAATTGC+AGG | - | chr4_2:54086267-54086286 | None:intergenic | 40.0% | |
| CTGAGAAGTAAAACACATCC+TGG | - | chr4_2:54086356-54086375 | None:intergenic | 40.0% | |
| ATTAAAAGTGGTATCCCTCG+AGG | - | chr4_2:54086387-54086406 | None:intergenic | 40.0% | |
| TGATTGCACATTTGACACCA+GGG | - | chr4_2:54086434-54086453 | None:intergenic | 40.0% | |
| TTGATTGCACATTTGACACC+AGG | - | chr4_2:54086435-54086454 | None:intergenic | 40.0% | |
| AATGTTGACATTTCAGGGCT+TGG | - | chr4_2:54086462-54086481 | None:intergenic | 40.0% | |
| ! | TCTGGAACATAACCATAGGA+AGG | - | chr4_2:54085825-54085844 | None:intergenic | 40.0% |
| CATCCTCGGTAAAAAATGCT+TGG | - | chr4_2:54085939-54085958 | None:intergenic | 40.0% | |
| !!! | CAAGCATTTTTTACCGAGGA+TGG | + | chr4_2:54085937-54085956 | Msa0607290:CDS | 40.0% |
| ! | TTTTACCGAGGATGGTAGAA+TGG | + | chr4_2:54085945-54085964 | Msa0607290:CDS | 40.0% |
| GGTATATACCTCAAGGAAAG+CGG | - | chr4_2:54085977-54085996 | None:intergenic | 40.0% | |
| CATTGAATGTGTTGCAGACA+TGG | + | chr4_2:54086202-54086221 | Msa0607290:intron | 40.0% | |
| GACTTCCCTGCAATTGTAAT+TGG | + | chr4_2:54086258-54086277 | Msa0607290:CDS | 40.0% | |
| TTCCCTGCAATTGTAATTGG+TGG | + | chr4_2:54086261-54086280 | Msa0607290:CDS | 40.0% | |
| CACCACCAATTACAATTGCA+GGG | - | chr4_2:54086266-54086285 | None:intergenic | 40.0% | |
| ACACCACCAATTACAATTGC+AGG | - | chr4_2:54086267-54086286 | None:intergenic | 40.0% | |
| CTGAGAAGTAAAACACATCC+TGG | - | chr4_2:54086356-54086375 | None:intergenic | 40.0% | |
| ATTAAAAGTGGTATCCCTCG+AGG | - | chr4_2:54086387-54086406 | None:intergenic | 40.0% | |
| TGATTGCACATTTGACACCA+GGG | - | chr4_2:54086434-54086453 | None:intergenic | 40.0% | |
| TTGATTGCACATTTGACACC+AGG | - | chr4_2:54086435-54086454 | None:intergenic | 40.0% | |
| AATGTTGACATTTCAGGGCT+TGG | - | chr4_2:54086462-54086481 | None:intergenic | 40.0% | |
| ATGTTCCAGAACATGTCCCA+AGG | + | chr4_2:54085835-54085854 | Msa0607290:CDS | 45.0% | |
| ACCTGTAAAACTGAGCTAGG+CGG | - | chr4_2:54085862-54085881 | None:intergenic | 45.0% | |
| GCAACCTGTAAAACTGAGCT+AGG | - | chr4_2:54085865-54085884 | None:intergenic | 45.0% | |
| AAAACAACCAACAGCCTGTG+TGG | + | chr4_2:54085890-54085909 | Msa0607290:CDS | 45.0% | |
| TGAAATACCACACAGGCTGT+TGG | - | chr4_2:54085900-54085919 | None:intergenic | 45.0% | |
| TGAATGTGTTGCAGACATGG+AGG | + | chr4_2:54086205-54086224 | Msa0607290:intron | 45.0% | |
| TACAATTGCAGGGAAGTCCT+TGG | - | chr4_2:54086256-54086275 | None:intergenic | 45.0% | |
| AAAGCGTAAGAATGCGAACC+AGG | + | chr4_2:54086335-54086354 | Msa0607290:CDS | 45.0% | |
| ATCGAACTTACCACAGTGGT+TGG | + | chr4_2:54086405-54086424 | Msa0607290:CDS | 45.0% | |
| AAGCCATCGAGTCTCTTCTT+AGG | + | chr4_2:54086490-54086509 | Msa0607290:CDS | 45.0% | |
| ACTCCTAAGAAGAGACTCGA+TGG | - | chr4_2:54086496-54086515 | None:intergenic | 45.0% | |
| ATGTTCCAGAACATGTCCCA+AGG | + | chr4_2:54085835-54085854 | Msa0607290:CDS | 45.0% | |
| ACCTGTAAAACTGAGCTAGG+CGG | - | chr4_2:54085862-54085881 | None:intergenic | 45.0% | |
| GCAACCTGTAAAACTGAGCT+AGG | - | chr4_2:54085865-54085884 | None:intergenic | 45.0% | |
| AAAACAACCAACAGCCTGTG+TGG | + | chr4_2:54085890-54085909 | Msa0607290:CDS | 45.0% | |
| TGAAATACCACACAGGCTGT+TGG | - | chr4_2:54085900-54085919 | None:intergenic | 45.0% | |
| TGAATGTGTTGCAGACATGG+AGG | + | chr4_2:54086205-54086224 | Msa0607290:intron | 45.0% | |
| TACAATTGCAGGGAAGTCCT+TGG | - | chr4_2:54086256-54086275 | None:intergenic | 45.0% | |
| AAAGCGTAAGAATGCGAACC+AGG | + | chr4_2:54086335-54086354 | Msa0607290:CDS | 45.0% | |
| ATCGAACTTACCACAGTGGT+TGG | + | chr4_2:54086405-54086424 | Msa0607290:CDS | 45.0% | |
| AAGCCATCGAGTCTCTTCTT+AGG | + | chr4_2:54086490-54086509 | Msa0607290:CDS | 45.0% | |
| ACTCCTAAGAAGAGACTCGA+TGG | - | chr4_2:54086496-54086515 | None:intergenic | 45.0% | |
| AGCTAGGACACTCCTTCCTA+TGG | + | chr4_2:54085810-54085829 | Msa0607290:exon | 50.0% | |
| ! | TCCGCCTAGCTCAGTTTTAC+AGG | + | chr4_2:54085858-54085877 | Msa0607290:CDS | 50.0% |
| ACGCTCCATTCTACCATCCT+CGG | - | chr4_2:54085953-54085972 | None:intergenic | 50.0% | |
| TTCTCAGCTAAACTGCCTCG+AGG | + | chr4_2:54086369-54086388 | Msa0607290:CDS | 50.0% | |
| TCTCAGCTAAACTGCCTCGA+GGG | + | chr4_2:54086370-54086389 | Msa0607290:CDS | 50.0% | |
| AGCTAGGACACTCCTTCCTA+TGG | + | chr4_2:54085810-54085829 | Msa0607290:exon | 50.0% | |
| ! | TCCGCCTAGCTCAGTTTTAC+AGG | + | chr4_2:54085858-54085877 | Msa0607290:CDS | 50.0% |
| ACGCTCCATTCTACCATCCT+CGG | - | chr4_2:54085953-54085972 | None:intergenic | 50.0% | |
| TTCTCAGCTAAACTGCCTCG+AGG | + | chr4_2:54086369-54086388 | Msa0607290:CDS | 50.0% | |
| TCTCAGCTAAACTGCCTCGA+GGG | + | chr4_2:54086370-54086389 | Msa0607290:CDS | 50.0% | |
| AACTGAGCTAGGCGGACCTT+GGG | - | chr4_2:54085854-54085873 | None:intergenic | 55.0% | |
| AAACTGAGCTAGGCGGACCT+TGG | - | chr4_2:54085855-54085874 | None:intergenic | 55.0% | |
| ACCACAGTGGTTGGAAACCC+TGG | + | chr4_2:54086414-54086433 | Msa0607290:CDS | 55.0% | |
| ACCAGGGTTTCCAACCACTG+TGG | - | chr4_2:54086418-54086437 | None:intergenic | 55.0% | |
| AACTGAGCTAGGCGGACCTT+GGG | - | chr4_2:54085854-54085873 | None:intergenic | 55.0% | |
| AAACTGAGCTAGGCGGACCT+TGG | - | chr4_2:54085855-54085874 | None:intergenic | 55.0% | |
| ACCACAGTGGTTGGAAACCC+TGG | + | chr4_2:54086414-54086433 | Msa0607290:CDS | 55.0% | |
| ACCAGGGTTTCCAACCACTG+TGG | - | chr4_2:54086418-54086437 | None:intergenic | 55.0% | |
| GCGGACCTTGGGACATGTTC+TGG | - | chr4_2:54085843-54085862 | None:intergenic | 60.0% | |
| !! | TGGTGGTGTTGACGCAACCG+TGG | + | chr4_2:54086278-54086297 | Msa0607290:CDS | 60.0% |
| TGACGCAACCGTGGAACGAC+TGG | + | chr4_2:54086287-54086306 | Msa0607290:CDS | 60.0% | |
| TGATGCGGCCAGTCGTTCCA+CGG | - | chr4_2:54086298-54086317 | None:intergenic | 60.0% | |
| GCGGACCTTGGGACATGTTC+TGG | - | chr4_2:54085843-54085862 | None:intergenic | 60.0% | |
| !! | TGGTGGTGTTGACGCAACCG+TGG | + | chr4_2:54086278-54086297 | Msa0607290:CDS | 60.0% |
| TGACGCAACCGTGGAACGAC+TGG | + | chr4_2:54086287-54086306 | Msa0607290:CDS | 60.0% | |
| TGATGCGGCCAGTCGTTCCA+CGG | - | chr4_2:54086298-54086317 | None:intergenic | 60.0% | |
| !! | GGAGCGTGCCGCTTTCCTTG+AGG | + | chr4_2:54085966-54085985 | Msa0607290:CDS | 65.0% |
| !! | GGAGCGTGCCGCTTTCCTTG+AGG | + | chr4_2:54085966-54085985 | Msa0607290:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 54085806 | 54086758 | 54085806 | ID=Msa0607290;Name=Msa0607290 |
| chr4_2 | mRNA | 54085806 | 54086758 | 54085806 | ID=Msa0607290-mRNA-1;Parent=Msa0607290;Name=Msa0607290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=23|1|1|1|1|1|2|240|152 |
| chr4_2 | exon | 54085806 | 54085987 | 54085806 | ID=Msa0607290-mRNA-1:exon:13440;Parent=Msa0607290-mRNA-1 |
| chr4_2 | exon | 54086219 | 54086758 | 54086219 | ID=Msa0607290-mRNA-1:exon:13441;Parent=Msa0607290-mRNA-1 |
| chr4_2 | five_prime_UTR | 54085806 | 54085828 | 54085806 | ID=Msa0607290-mRNA-1:five_prime_utr;Parent=Msa0607290-mRNA-1 |
| chr4_2 | CDS | 54085829 | 54085987 | 54085829 | ID=Msa0607290-mRNA-1:cds;Parent=Msa0607290-mRNA-1 |
| chr4_2 | CDS | 54086219 | 54086518 | 54086219 | ID=Msa0607290-mRNA-1:cds;Parent=Msa0607290-mRNA-1 |
| chr4_2 | three_prime_UTR | 54086519 | 54086758 | 54086519 | ID=Msa0607290-mRNA-1:three_prime_utr;Parent=Msa0607290-mRNA-1 |
| Gene Sequence |
| Protein sequence |
