AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1286680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286680 MtrunA17_Chr8g0360861 99.083 109 1 0 1 109 1 109 2.40e-77 223
Msa1286680 MtrunA17_Chr6g0485891 50.538 93 46 0 1 93 35 127 5.62e-30 103
Msa1286680 MtrunA17_Chr2g0331651 55.556 90 40 0 1 90 1 90 9.84e-30 102
Msa1286680 MtrunA17_Chr4g0067531 48.837 86 42 1 5 90 46 129 2.39e-26 94.7
Msa1286680 MtrunA17_Chr4g0063491 49.367 79 40 0 11 89 159 237 4.00e-22 87.0
Msa1286680 MtrunA17_Chr8g0391451 45.882 85 44 1 12 94 60 144 1.16e-19 78.2
Msa1286680 MtrunA17_Chr4g0059791 50.633 79 38 1 12 89 222 300 1.37e-18 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286680 AT4G27360.1 71.845 103 29 0 1 103 1 103 1.71e-50 155
Msa1286680 AT3G16120.2 55.435 92 41 0 1 92 1 92 4.93e-33 110
Msa1286680 AT3G16120.1 55.435 92 41 0 1 92 1 92 4.93e-33 110
Msa1286680 AT1G52245.1 51.064 94 46 0 1 94 1 94 3.30e-30 103
Msa1286680 AT4G15930.1 49.412 85 41 1 5 89 39 121 3.59e-24 89.4
Msa1286680 AT5G20110.1 46.988 83 44 0 12 94 123 205 2.79e-21 84.3
Msa1286680 AT1G23220.1 39.773 88 52 1 8 94 38 125 4.12e-18 74.3
Msa1286680 AT4G15930.2 49.180 61 29 1 5 65 39 97 1.34e-13 61.6

Find 28 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATTGTAGGCACTGATTT+TGG 0.221609 8_3:-35340822 Msa1286680:CDS
GCACAAGAGTAGAACAAAAT+AGG 0.295751 8_3:+35341165 None:intergenic
CAGGAATTTGATAGGGTTCA+TGG 0.298316 8_3:-35340858 Msa1286680:CDS
CAGTTACATCATAGAAATCT+AGG 0.300776 8_3:+35341002 None:intergenic
AATTATTGCAGGAATTTGAT+AGG 0.302502 8_3:-35340866 Msa1286680:intron
TTAGCCCTCACAAAAGAAAA+AGG 0.351072 8_3:+35340541 None:intergenic
ATCATAGAAATCTAGGGCTT+TGG 0.353606 8_3:+35341009 None:intergenic
CTTCAATTCATCATGCTAGA+AGG 0.354098 8_3:-35341097 Msa1286680:exon
AATAGTAATGTAGAAAGAAA+TGG 0.408786 8_3:-35340614 Msa1286680:three_prime_UTR
TTGCTCTTTAAGGGATCTGC+TGG 0.420352 8_3:-35340738 Msa1286680:CDS
CCTGGTTGGCAATGCATTGT+AGG 0.440795 8_3:-35340834 Msa1286680:CDS
TTGCATAGTTTGAAGCATAT+CGG 0.449905 8_3:+35341054 None:intergenic
CTAGAAGGCAAGGCAGTGAT+TGG 0.481296 8_3:-35341082 Msa1286680:CDS
CCTACAATGCATTGCCAACC+AGG 0.504627 8_3:+35340834 None:intergenic
AAATCAGTTCTCAGCAATAG+AGG 0.512873 8_3:-35340700 Msa1286680:CDS
TATAGCTCGATTTATAAAGA+AGG 0.514762 8_3:-35340969 Msa1286680:intron
TTTGATAGGGTTCATGGACC+TGG 0.522418 8_3:-35340852 Msa1286680:CDS
ATTATTGCAGGAATTTGATA+GGG 0.524356 8_3:-35340865 Msa1286680:intron
TGTGTACAGTCGAAGCTACA+TGG 0.533055 8_3:-35340510 Msa1286680:three_prime_UTR
TATAAATCGAGCTATATCAA+TGG 0.561045 8_3:+35340976 None:intergenic
ATCTGCTGGTCCACAAGCAG+AGG 0.571115 8_3:-35340724 Msa1286680:CDS
ATTCATCATGCTAGAAGGCA+AGG 0.581622 8_3:-35341092 Msa1286680:exon
TCCTTTGTGACACATTGTAG+TGG 0.583602 8_3:-35340798 Msa1286680:CDS
AGTTACATCATAGAAATCTA+GGG 0.585718 8_3:+35341003 None:intergenic
ATAGGGTTCATGGACCTGGT+TGG 0.594187 8_3:-35340848 Msa1286680:CDS
GCCACTACAATGTGTCACAA+AGG 0.659746 8_3:+35340797 None:intergenic
GTGTACAGTCGAAGCTACAT+GGG 0.667081 8_3:-35340509 Msa1286680:three_prime_UTR
AGATTTCTATGATGTAACTG+AGG 0.680263 8_3:-35340999 Msa1286680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATGTAATTTTTTTTG+TGG - chr8_3:35340723-35340742 Msa1286680:CDS 10.0%
!! AAGATTTGAAAAATAAAAAA+TGG - chr8_3:35341044-35341063 Msa1286680:CDS 10.0%
!!! TTTTAATGTAATTTTTTTTG+TGG - chr8_3:35340723-35340742 Msa1286680:CDS 10.0%
!! AAGATTTGAAAAATAAAAAA+TGG - chr8_3:35341044-35341063 Msa1286680:CDS 10.0%
!! AACCAATAAAAAAAAAAAAG+TGG - chr8_3:35340421-35340440 Msa1286680:three_prime_UTR 15.0%
!!! TACTTTTTTTTTTTGAAGAA+AGG + chr8_3:35340447-35340466 None:intergenic 15.0%
!! AAATTTACTACAAAAATAAG+AGG + chr8_3:35340514-35340533 None:intergenic 15.0%
!!! TAGTATTAATTTTGTTTTTG+AGG - chr8_3:35341162-35341181 Msa1286680:five_prime_UTR 15.0%
!!! AGTATTAATTTTGTTTTTGA+GGG - chr8_3:35341163-35341182 Msa1286680:five_prime_UTR 15.0%
!! AACCAATAAAAAAAAAAAAG+TGG - chr8_3:35340421-35340440 Msa1286680:three_prime_UTR 15.0%
!!! TACTTTTTTTTTTTGAAGAA+AGG + chr8_3:35340447-35340466 None:intergenic 15.0%
!! AAATTTACTACAAAAATAAG+AGG + chr8_3:35340514-35340533 None:intergenic 15.0%
!!! TAGTATTAATTTTGTTTTTG+AGG - chr8_3:35341162-35341181 Msa1286680:five_prime_UTR 15.0%
!!! AGTATTAATTTTGTTTTTGA+GGG - chr8_3:35341163-35341182 Msa1286680:five_prime_UTR 15.0%
!! GGTAATAAATTAAAACTAAG+AGG - chr8_3:35340685-35340704 Msa1286680:exon 20.0%
!! AATAGTAATGTAGAAAGAAA+TGG - chr8_3:35341019-35341038 Msa1286680:CDS 20.0%
!! AAAAGAAAAAGGCAATAAAA+TGG + chr8_3:35341084-35341103 None:intergenic 20.0%
!!! GTATTAATTTTGTTTTTGAG+GGG - chr8_3:35341164-35341183 Msa1286680:five_prime_UTR 20.0%
!!! TATTAATTTTGTTTTTGAGG+GGG - chr8_3:35341165-35341184 Msa1286680:five_prime_UTR 20.0%
!! GGTAATAAATTAAAACTAAG+AGG - chr8_3:35340685-35340704 Msa1286680:exon 20.0%
!! AATAGTAATGTAGAAAGAAA+TGG - chr8_3:35341019-35341038 Msa1286680:CDS 20.0%
!! AAAAGAAAAAGGCAATAAAA+TGG + chr8_3:35341084-35341103 None:intergenic 20.0%
!!! GTATTAATTTTGTTTTTGAG+GGG - chr8_3:35341164-35341183 Msa1286680:five_prime_UTR 20.0%
!!! TATTAATTTTGTTTTTGAGG+GGG - chr8_3:35341165-35341184 Msa1286680:five_prime_UTR 20.0%
!!! GGCCACTTTTTTTTTTTTAT+TGG + chr8_3:35340426-35340445 None:intergenic 25.0%
! AGTTACATCATAGAAATCTA+GGG + chr8_3:35340633-35340652 None:intergenic 25.0%
! TATAAATCGAGCTATATCAA+TGG + chr8_3:35340660-35340679 None:intergenic 25.0%
! TATAGCTCGATTTATAAAGA+AGG - chr8_3:35340664-35340683 Msa1286680:three_prime_UTR 25.0%
! AAACTAAGAGGATTGTTTAT+TGG - chr8_3:35340697-35340716 Msa1286680:CDS 25.0%
! GATATGTTGTTAATTATTGC+AGG - chr8_3:35340756-35340775 Msa1286680:CDS 25.0%
! AATTATTGCAGGAATTTGAT+AGG - chr8_3:35340767-35340786 Msa1286680:CDS 25.0%
! ATTATTGCAGGAATTTGATA+GGG - chr8_3:35340768-35340787 Msa1286680:CDS 25.0%
!!! TGCTTCATCTATTTTTGTAT+AGG - chr8_3:35340859-35340878 Msa1286680:intron 25.0%
!!! CTATTTTTGTATAGGTAGTA+TGG - chr8_3:35340867-35340886 Msa1286680:intron 25.0%
!! GATAGTATCAATTTTCTTAC+AGG - chr8_3:35341191-35341210 Msa1286680:five_prime_UTR 25.0%
!!! GGCCACTTTTTTTTTTTTAT+TGG + chr8_3:35340426-35340445 None:intergenic 25.0%
! AGTTACATCATAGAAATCTA+GGG + chr8_3:35340633-35340652 None:intergenic 25.0%
! TATAAATCGAGCTATATCAA+TGG + chr8_3:35340660-35340679 None:intergenic 25.0%
! TATAGCTCGATTTATAAAGA+AGG - chr8_3:35340664-35340683 Msa1286680:three_prime_UTR 25.0%
! AAACTAAGAGGATTGTTTAT+TGG - chr8_3:35340697-35340716 Msa1286680:CDS 25.0%
! GATATGTTGTTAATTATTGC+AGG - chr8_3:35340756-35340775 Msa1286680:CDS 25.0%
! AATTATTGCAGGAATTTGAT+AGG - chr8_3:35340767-35340786 Msa1286680:CDS 25.0%
! ATTATTGCAGGAATTTGATA+GGG - chr8_3:35340768-35340787 Msa1286680:CDS 25.0%
!!! TGCTTCATCTATTTTTGTAT+AGG - chr8_3:35340859-35340878 Msa1286680:intron 25.0%
!!! CTATTTTTGTATAGGTAGTA+TGG - chr8_3:35340867-35340886 Msa1286680:intron 25.0%
!! GATAGTATCAATTTTCTTAC+AGG - chr8_3:35341191-35341210 Msa1286680:five_prime_UTR 25.0%
! TTGCATAGTTTGAAGCATAT+CGG + chr8_3:35340582-35340601 None:intergenic 30.0%
CAGTTACATCATAGAAATCT+AGG + chr8_3:35340634-35340653 None:intergenic 30.0%
AGATTTCTATGATGTAACTG+AGG - chr8_3:35340634-35340653 Msa1286680:three_prime_UTR 30.0%
! TATGGCAATTTTGCTCTTTA+AGG - chr8_3:35340885-35340904 Msa1286680:intron 30.0%
! ATGGCAATTTTGCTCTTTAA+GGG - chr8_3:35340886-35340905 Msa1286680:intron 30.0%
! TATTGCCTTTTTCTTTTGTG+AGG - chr8_3:35341087-35341106 Msa1286680:CDS 30.0%
! ATTGCCTTTTTCTTTTGTGA+GGG - chr8_3:35341088-35341107 Msa1286680:CDS 30.0%
! TTGCATAGTTTGAAGCATAT+CGG + chr8_3:35340582-35340601 None:intergenic 30.0%
CAGTTACATCATAGAAATCT+AGG + chr8_3:35340634-35340653 None:intergenic 30.0%
AGATTTCTATGATGTAACTG+AGG - chr8_3:35340634-35340653 Msa1286680:three_prime_UTR 30.0%
! TATGGCAATTTTGCTCTTTA+AGG - chr8_3:35340885-35340904 Msa1286680:intron 30.0%
! ATGGCAATTTTGCTCTTTAA+GGG - chr8_3:35340886-35340905 Msa1286680:intron 30.0%
! TATTGCCTTTTTCTTTTGTG+AGG - chr8_3:35341087-35341106 Msa1286680:CDS 30.0%
! ATTGCCTTTTTCTTTTGTGA+GGG - chr8_3:35341088-35341107 Msa1286680:CDS 30.0%
GCACAAGAGTAGAACAAAAT+AGG + chr8_3:35340471-35340490 None:intergenic 35.0%
CTTCAATTCATCATGCTAGA+AGG - chr8_3:35340536-35340555 Msa1286680:three_prime_UTR 35.0%
! ATCATAGAAATCTAGGGCTT+TGG + chr8_3:35340627-35340646 None:intergenic 35.0%
AAATCAGTTCTCAGCAATAG+AGG - chr8_3:35340933-35340952 Msa1286680:intron 35.0%
TTAGCCCTCACAAAAGAAAA+AGG + chr8_3:35341095-35341114 None:intergenic 35.0%
GCACAAGAGTAGAACAAAAT+AGG + chr8_3:35340471-35340490 None:intergenic 35.0%
CTTCAATTCATCATGCTAGA+AGG - chr8_3:35340536-35340555 Msa1286680:three_prime_UTR 35.0%
! ATCATAGAAATCTAGGGCTT+TGG + chr8_3:35340627-35340646 None:intergenic 35.0%
AAATCAGTTCTCAGCAATAG+AGG - chr8_3:35340933-35340952 Msa1286680:intron 35.0%
TTAGCCCTCACAAAAGAAAA+AGG + chr8_3:35341095-35341114 None:intergenic 35.0%
ATTCATCATGCTAGAAGGCA+AGG - chr8_3:35340541-35340560 Msa1286680:three_prime_UTR 40.0%
CAGGAATTTGATAGGGTTCA+TGG - chr8_3:35340775-35340794 Msa1286680:CDS 40.0%
!!! TGCATTGTAGGCACTGATTT+TGG - chr8_3:35340811-35340830 Msa1286680:CDS 40.0%
TCCTTTGTGACACATTGTAG+TGG - chr8_3:35340835-35340854 Msa1286680:CDS 40.0%
!! AACTGATTTTCCTCTGCTTG+TGG + chr8_3:35340922-35340941 None:intergenic 40.0%
ATTCATCATGCTAGAAGGCA+AGG - chr8_3:35340541-35340560 Msa1286680:three_prime_UTR 40.0%
CAGGAATTTGATAGGGTTCA+TGG - chr8_3:35340775-35340794 Msa1286680:CDS 40.0%
!!! TGCATTGTAGGCACTGATTT+TGG - chr8_3:35340811-35340830 Msa1286680:CDS 40.0%
TCCTTTGTGACACATTGTAG+TGG - chr8_3:35340835-35340854 Msa1286680:CDS 40.0%
!! AACTGATTTTCCTCTGCTTG+TGG + chr8_3:35340922-35340941 None:intergenic 40.0%
TTTGATAGGGTTCATGGACC+TGG - chr8_3:35340781-35340800 Msa1286680:CDS 45.0%
GCCACTACAATGTGTCACAA+AGG + chr8_3:35340839-35340858 None:intergenic 45.0%
! TTGCTCTTTAAGGGATCTGC+TGG - chr8_3:35340895-35340914 Msa1286680:intron 45.0%
TGTGTACAGTCGAAGCTACA+TGG - chr8_3:35341123-35341142 Msa1286680:five_prime_UTR 45.0%
GTGTACAGTCGAAGCTACAT+GGG - chr8_3:35341124-35341143 Msa1286680:five_prime_UTR 45.0%
TTTGATAGGGTTCATGGACC+TGG - chr8_3:35340781-35340800 Msa1286680:CDS 45.0%
GCCACTACAATGTGTCACAA+AGG + chr8_3:35340839-35340858 None:intergenic 45.0%
! TTGCTCTTTAAGGGATCTGC+TGG - chr8_3:35340895-35340914 Msa1286680:intron 45.0%
TGTGTACAGTCGAAGCTACA+TGG - chr8_3:35341123-35341142 Msa1286680:five_prime_UTR 45.0%
GTGTACAGTCGAAGCTACAT+GGG - chr8_3:35341124-35341143 Msa1286680:five_prime_UTR 45.0%
CTAGAAGGCAAGGCAGTGAT+TGG - chr8_3:35340551-35340570 Msa1286680:three_prime_UTR 50.0%
ATAGGGTTCATGGACCTGGT+TGG - chr8_3:35340785-35340804 Msa1286680:CDS 50.0%
CCTACAATGCATTGCCAACC+AGG + chr8_3:35340802-35340821 None:intergenic 50.0%
CCTGGTTGGCAATGCATTGT+AGG - chr8_3:35340799-35340818 Msa1286680:CDS 50.0%
CTAGAAGGCAAGGCAGTGAT+TGG - chr8_3:35340551-35340570 Msa1286680:three_prime_UTR 50.0%
ATAGGGTTCATGGACCTGGT+TGG - chr8_3:35340785-35340804 Msa1286680:CDS 50.0%
CCTACAATGCATTGCCAACC+AGG + chr8_3:35340802-35340821 None:intergenic 50.0%
CCTGGTTGGCAATGCATTGT+AGG - chr8_3:35340799-35340818 Msa1286680:CDS 50.0%
ATCTGCTGGTCCACAAGCAG+AGG - chr8_3:35340909-35340928 Msa1286680:intron 55.0%
ATCTGCTGGTCCACAAGCAG+AGG - chr8_3:35340909-35340928 Msa1286680:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 35340416 35341239 35340416 ID=Msa1286680;Name=Msa1286680
chr8_3 mRNA 35340416 35341239 35340416 ID=Msa1286680-mRNA-1;Parent=Msa1286680;Name=Msa1286680-mRNA-1;_AED=0.02;_eAED=0.02;_QI=132|1|1|1|1|1|2|270|109
chr8_3 exon 35340416 35340877 35340416 ID=Msa1286680-mRNA-1:exon:10402;Parent=Msa1286680-mRNA-1
chr8_3 exon 35340970 35341239 35340970 ID=Msa1286680-mRNA-1:exon:10401;Parent=Msa1286680-mRNA-1
chr8_3 five_prime_UTR 35341108 35341239 35341108 ID=Msa1286680-mRNA-1:five_prime_utr;Parent=Msa1286680-mRNA-1
chr8_3 CDS 35340970 35341107 35340970 ID=Msa1286680-mRNA-1:cds;Parent=Msa1286680-mRNA-1
chr8_3 CDS 35340686 35340877 35340686 ID=Msa1286680-mRNA-1:cds;Parent=Msa1286680-mRNA-1
chr8_3 three_prime_UTR 35340416 35340685 35340416 ID=Msa1286680-mRNA-1:three_prime_utr;Parent=Msa1286680-mRNA-1
Gene Sequence

>Msa1286680

ATGCTAGAAGGCAAGGCAGTGATTGGTGAGACCGATATGCTTCAAACTATGCAACAAGATGCTCTTGATTTAGCATCCAAAGCCCTAGATTTCTATGATGTAACTGAGGCCATTGATATAGCTCGATTTATAAAGAAGGAATTTGATAGGGTTCATGGACCTGGTTGGCAATGCATTGTAGGCACTGATTTTGGTTCCTTTGTGACACATTGTAGTGGCTGCTTCATCTATTTTTGTATAGGTAGTATGGCAATTTTGCTCTTTAAGGGATCTGCTGGTCCACAAGCAGAGGAAAATCAGTTCTCAGCAATAGAGGCAACTAAAGCATAA

Protein sequence

>Msa1286680

MLEGKAVIGETDMLQTMQQDALDLASKALDFYDVTEAIDIARFIKKEFDRVHGPGWQCIVGTDFGSFVTHCSGCFIYFCIGSMAILLFKGSAGPQAEENQFSAIEATKA*