AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1294900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294900 MtrunA17_Chr8g0372141 96.891 193 6 0 1 193 1 193 1.08e-140 390
Msa1294900 MtrunA17_Chr5g0405171 76.923 195 42 3 1 193 14 207 6.71e-108 307
Msa1294900 MtrunA17_Chr2g0277151 63.087 149 49 2 12 160 2 144 1.50e-64 197
Msa1294900 MtrunA17_Chr4g0063501 57.639 144 57 1 12 155 4 143 1.82e-56 176
Msa1294900 MtrunA17_Chr4g0003581 53.691 149 62 2 12 157 8 152 2.73e-55 173
Msa1294900 MtrunA17_Chr4g0020201 47.183 142 73 2 14 154 35 175 1.08e-47 154
Msa1294900 MtrunA17_Chr2g0332851 49.333 150 68 3 12 157 8 153 3.17e-47 152
Msa1294900 MtrunA17_Chr2g0332841 48.667 150 69 3 12 157 8 153 1.32e-45 148
Msa1294900 MtrunA17_Chr1g0212241 44.444 135 66 3 30 161 3 131 1.87e-36 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294900 AT1G15380.2 56.376 149 58 2 12 157 8 152 1.84e-57 179
Msa1294900 AT1G15380.1 56.376 149 58 2 12 157 8 152 1.84e-57 179
Msa1294900 AT1G80160.1 51.678 149 65 2 12 157 8 152 1.34e-54 171
Msa1294900 AT1G80160.3 51.678 149 65 2 12 157 49 193 3.99e-54 171
Msa1294900 AT2G28420.1 49.306 144 69 2 14 156 17 157 1.36e-48 156
Msa1294900 AT1G80160.2 45.217 115 56 2 57 168 27 137 1.66e-31 111

Find 72 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCAAATTCATCATAATT+TGG 0.184282 8_3:+52840385 None:intergenic
TTGTGTAGGTTGTATAATTA+TGG 0.227214 8_3:-52840433 Msa1294900:intron
ATGGTTGTCCTTGGGATTAA+TGG 0.258018 8_3:+52840342 None:intergenic
TAATTACTTGGAATGATATA+TGG 0.268558 8_3:+52840323 None:intergenic
TGTTCTTCAAAACAAGTAAA+AGG 0.269814 8_3:-52839792 Msa1294900:three_prime_UTR
ATTTAGTGGCTGGGTATATA+TGG 0.299391 8_3:+52839533 None:intergenic
ACCATAGTTCAACATTAATA+TGG 0.304305 8_3:+52839865 None:intergenic
TAAGGGAGAATATTGGAAAA+TGG 0.308317 8_3:-52840724 Msa1294900:exon
TATGAAGAAATCTTGGGTTT+TGG 0.309204 8_3:-52840603 Msa1294900:CDS
TGTGTAGGTTGTATAATTAT+GGG 0.310343 8_3:-52840432 Msa1294900:intron
CAACACTGATCTGCATAATA+TGG 0.311667 8_3:+52840641 None:intergenic
TTCTAACAAGTGTGGATTCA+TGG 0.313847 8_3:-52839946 Msa1294900:CDS
GCAGAAGAAATGGGAACTAT+TGG 0.335608 8_3:+52840014 None:intergenic
ATGGGTCGTGATTCACTCAT+AGG 0.351235 8_3:+52840361 None:intergenic
GAAAATGAGGTTGGAAGAAA+TGG 0.352191 8_3:-52840147 Msa1294900:CDS
CCTTCCTCTTTCAAATTCAA+TGG 0.370841 8_3:-52840567 Msa1294900:CDS
GTGTGTTTGAAATAATAAAA+TGG 0.374055 8_3:-52839713 Msa1294900:three_prime_UTR
GGAGCAGGAAGCAGAAGAAA+TGG 0.375124 8_3:+52840004 None:intergenic
AAGGGAGAATATTGGAAAAT+GGG 0.376216 8_3:-52840723 Msa1294900:exon
TCCATATTAATGTTGAACTA+TGG 0.376542 8_3:-52839866 Msa1294900:three_prime_UTR
AAAATGGGGATTGAGGAAAT+TGG 0.400288 8_3:-52840708 Msa1294900:CDS
TGGTTGTCCTTGGGATTAAT+GGG 0.407514 8_3:+52840343 None:intergenic
AGGTTGTATAATTATGGGTT+TGG 0.407784 8_3:-52840427 Msa1294900:CDS
CAGAAGAAATGGGAACTATT+GGG 0.429676 8_3:+52840015 None:intergenic
AGTTCTATCATGTATCCATC+AGG 0.435736 8_3:+52840056 None:intergenic
GATGAAGTATGTTACGGCTT+TGG 0.445021 8_3:-52840123 Msa1294900:CDS
AAACAGTCTTCTTAAAGCTC+TGG 0.447084 8_3:+52839967 None:intergenic
GAATGATATATGGTTGTCCT+TGG 0.451197 8_3:+52840333 None:intergenic
AAAATGAGGTTGGAAGAAAT+GGG 0.458153 8_3:-52840146 Msa1294900:CDS
GCTCTGGCCTCGTGACTTGA+AGG 0.458217 8_3:+52839983 None:intergenic
TTGTGCAGTGTACAGATGTT+GGG 0.460233 8_3:-52840175 Msa1294900:intron
CATAATTTGGATTCTCAAGC+AGG 0.462581 8_3:+52840398 None:intergenic
TGCTCCATTGAATTTGAAAG+AGG 0.466642 8_3:+52840563 None:intergenic
CACATGATTCAGTGAGAGAA+GGG 0.472092 8_3:+52840665 None:intergenic
TTTGTGCAGTGTACAGATGT+TGG 0.472216 8_3:-52840176 Msa1294900:intron
CTGAGTGAAGAAGTGTCCTA+AGG 0.490013 8_3:-52840742 Msa1294900:five_prime_UTR
GTATGTTACGGCTTTGGTAG+AGG 0.491758 8_3:-52840117 Msa1294900:CDS
TGAAGGAATCAAGGTGGAGC+AGG 0.491848 8_3:-52840093 Msa1294900:CDS
AGTGTCCTAAGGGAGAATAT+TGG 0.492635 8_3:-52840731 Msa1294900:five_prime_UTR
TCAAACAATACAATTCAGAT+AGG 0.496925 8_3:+52839600 None:intergenic
TGGGTCGTGATTCACTCATA+GGG 0.496984 8_3:+52840362 None:intergenic
GCCTCGTGACTTGAAGGAGC+AGG 0.509012 8_3:+52839989 None:intergenic
GAGCAGGAAGCAGAAGAAAT+GGG 0.511553 8_3:+52840005 None:intergenic
AATGGGGATGAAGTATGTTA+CGG 0.515973 8_3:-52840129 Msa1294900:CDS
TCATGTATCCATCAGGGTCA+TGG 0.518219 8_3:+52840063 None:intergenic
CCATTGAATTTGAAAGAGGA+AGG 0.520942 8_3:+52840567 None:intergenic
AATGATATATGGTTGTCCTT+GGG 0.521545 8_3:+52840334 None:intergenic
ACGGCTTTGGTAGAGGATGA+AGG 0.527027 8_3:-52840110 Msa1294900:CDS
CTTTCAAATTCAATGGAGCA+TGG 0.540814 8_3:-52840560 Msa1294900:intron
TGAGTGAAGAAGTGTCCTAA+GGG 0.543669 8_3:-52840741 Msa1294900:five_prime_UTR
TTCAGTGAGAGAAGGGGTAG+TGG 0.543925 8_3:+52840672 None:intergenic
TGGGCTAGTGAAAATGAGGT+TGG 0.545299 8_3:-52840156 Msa1294900:CDS
GGTAGAGGATGAAGGAATCA+AGG 0.549754 8_3:-52840102 Msa1294900:CDS
CATATTATGCAGATCAGTGT+TGG 0.552916 8_3:-52840640 Msa1294900:CDS
GTGTTCTTCCATGACCCTGA+TGG 0.553401 8_3:-52840071 Msa1294900:CDS
ATATTGGAAAATGGGGATTG+AGG 0.566391 8_3:-52840715 Msa1294900:exon
CTGAATTGTATTGTTTGACA+AGG 0.576696 8_3:-52839595 Msa1294900:three_prime_UTR
AGGGAGAATATTGGAAAATG+GGG 0.577637 8_3:-52840722 Msa1294900:exon
AAGACTGTTTCTAACAAGTG+TGG 0.583686 8_3:-52839954 Msa1294900:CDS
ACACATGATTCAGTGAGAGA+AGG 0.601179 8_3:+52840664 None:intergenic
TCCTGCTCCTTCAAGTCACG+AGG 0.622464 8_3:-52839990 Msa1294900:CDS
GTTCTATCATGTATCCATCA+GGG 0.630489 8_3:+52840057 None:intergenic
CATTGAATTTGAAAGAGGAA+GGG 0.632398 8_3:+52840568 None:intergenic
TATGATGCGAAGCTTGAGCA+AGG 0.642219 8_3:-52839916 Msa1294900:CDS
ATCACGACCCATTAATCCCA+AGG 0.645670 8_3:-52840350 Msa1294900:CDS
ATGTTGGGCTAGTGAAAATG+AGG 0.654745 8_3:-52840160 Msa1294900:CDS
ATATTAATGTTGAACTATGG+TGG 0.668092 8_3:-52839863 Msa1294900:three_prime_UTR
GGGTCGTGATTCACTCATAG+GGG 0.689430 8_3:+52840363 None:intergenic
AAATGAGGTTGGAAGAAATG+GGG 0.690532 8_3:-52840145 Msa1294900:CDS
ACATGATTCAGTGAGAGAAG+GGG 0.696257 8_3:+52840666 None:intergenic
GATCAGTGTTGGACTCTGTG+AGG 0.702787 8_3:-52840629 Msa1294900:CDS
AGAGGATGAAGGAATCAAGG+TGG 0.720837 8_3:-52840099 Msa1294900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTTCATAAAGAAAA+TGG + chr8_3:52839812-52839831 None:intergenic 10.0%
!! ATAAAATTTCATAAAGAAAA+TGG + chr8_3:52839812-52839831 None:intergenic 10.0%
!! TATTTGTTCAAACAAAATAA+TGG + chr8_3:52839837-52839856 None:intergenic 15.0%
!!! CAAAAAATTTTATGAATATG+TGG - chr8_3:52840761-52840780 Msa1294900:five_prime_UTR 15.0%
!! TATTTGTTCAAACAAAATAA+TGG + chr8_3:52839837-52839856 None:intergenic 15.0%
!!! CAAAAAATTTTATGAATATG+TGG - chr8_3:52840761-52840780 Msa1294900:five_prime_UTR 15.0%
!!! TTTTTTTTTACGTATTAGCT+TGG + chr8_3:52839551-52839570 None:intergenic 20.0%
!!! TTTATTGTTTTGTTTTGTGT+AGG - chr8_3:52839872-52839891 Msa1294900:three_prime_UTR 20.0%
!! TAATTACTTGGAATGATATA+TGG + chr8_3:52839999-52840018 None:intergenic 20.0%
!!! GTGTGTTTGAAATAATAAAA+TGG - chr8_3:52840606-52840625 Msa1294900:CDS 20.0%
!!! CTTGTAACAAATTTTTAACA+GGG - chr8_3:52840651-52840670 Msa1294900:CDS 20.0%
!!! TTGTAACAAATTTTTAACAG+GGG - chr8_3:52840652-52840671 Msa1294900:CDS 20.0%
!!! TTTTTTTTTACGTATTAGCT+TGG + chr8_3:52839551-52839570 None:intergenic 20.0%
!!! TTTATTGTTTTGTTTTGTGT+AGG - chr8_3:52839872-52839891 Msa1294900:three_prime_UTR 20.0%
!! TAATTACTTGGAATGATATA+TGG + chr8_3:52839999-52840018 None:intergenic 20.0%
!!! GTGTGTTTGAAATAATAAAA+TGG - chr8_3:52840606-52840625 Msa1294900:CDS 20.0%
!!! CTTGTAACAAATTTTTAACA+GGG - chr8_3:52840651-52840670 Msa1294900:CDS 20.0%
!!! TTGTAACAAATTTTTAACAG+GGG - chr8_3:52840652-52840671 Msa1294900:CDS 20.0%
!!! AGGTTTTATGAAGAAATCTT+GGG - chr8_3:52839710-52839729 Msa1294900:three_prime_UTR 25.0%
! TTGTGTAGGTTGTATAATTA+TGG - chr8_3:52839886-52839905 Msa1294900:exon 25.0%
! TGTGTAGGTTGTATAATTAT+GGG - chr8_3:52839887-52839906 Msa1294900:exon 25.0%
! GCATCAAATTCATCATAATT+TGG + chr8_3:52839937-52839956 None:intergenic 25.0%
!!! TTACTGAAAGTGTTTCAATT+TGG - chr8_3:52840119-52840138 Msa1294900:CDS 25.0%
! TCCATATTAATGTTGAACTA+TGG - chr8_3:52840453-52840472 Msa1294900:intron 25.0%
! ACCATAGTTCAACATTAATA+TGG + chr8_3:52840457-52840476 None:intergenic 25.0%
! ATATTAATGTTGAACTATGG+TGG - chr8_3:52840456-52840475 Msa1294900:intron 25.0%
! TGTTCTTCAAAACAAGTAAA+AGG - chr8_3:52840527-52840546 Msa1294900:intron 25.0%
!! GCTTGTAACAAATTTTTAAC+AGG - chr8_3:52840650-52840669 Msa1294900:CDS 25.0%
!!! TGTTATTTTCTTCATTCGTA+GGG + chr8_3:52840698-52840717 None:intergenic 25.0%
! TCAAACAATACAATTCAGAT+AGG + chr8_3:52840722-52840741 None:intergenic 25.0%
!!! AGGTTTTATGAAGAAATCTT+GGG - chr8_3:52839710-52839729 Msa1294900:three_prime_UTR 25.0%
! TTGTGTAGGTTGTATAATTA+TGG - chr8_3:52839886-52839905 Msa1294900:exon 25.0%
! TGTGTAGGTTGTATAATTAT+GGG - chr8_3:52839887-52839906 Msa1294900:exon 25.0%
! GCATCAAATTCATCATAATT+TGG + chr8_3:52839937-52839956 None:intergenic 25.0%
!!! TTACTGAAAGTGTTTCAATT+TGG - chr8_3:52840119-52840138 Msa1294900:CDS 25.0%
! TCCATATTAATGTTGAACTA+TGG - chr8_3:52840453-52840472 Msa1294900:intron 25.0%
! ACCATAGTTCAACATTAATA+TGG + chr8_3:52840457-52840476 None:intergenic 25.0%
! ATATTAATGTTGAACTATGG+TGG - chr8_3:52840456-52840475 Msa1294900:intron 25.0%
! TGTTCTTCAAAACAAGTAAA+AGG - chr8_3:52840527-52840546 Msa1294900:intron 25.0%
!! GCTTGTAACAAATTTTTAAC+AGG - chr8_3:52840650-52840669 Msa1294900:CDS 25.0%
!!! TGTTATTTTCTTCATTCGTA+GGG + chr8_3:52840698-52840717 None:intergenic 25.0%
! TCAAACAATACAATTCAGAT+AGG + chr8_3:52840722-52840741 None:intergenic 25.0%
! ATTTTCCAATATTCTCCCTT+AGG + chr8_3:52839596-52839615 None:intergenic 30.0%
TAAGGGAGAATATTGGAAAA+TGG - chr8_3:52839595-52839614 Msa1294900:three_prime_UTR 30.0%
AAGGGAGAATATTGGAAAAT+GGG - chr8_3:52839596-52839615 Msa1294900:three_prime_UTR 30.0%
!! GAGGTTTTATGAAGAAATCT+TGG - chr8_3:52839709-52839728 Msa1294900:three_prime_UTR 30.0%
! TATGAAGAAATCTTGGGTTT+TGG - chr8_3:52839716-52839735 Msa1294900:three_prime_UTR 30.0%
CATTGAATTTGAAAGAGGAA+GGG + chr8_3:52839754-52839773 None:intergenic 30.0%
GGTAATGAAGCAAAGTATAT+AGG + chr8_3:52839791-52839810 None:intergenic 30.0%
AGGTTGTATAATTATGGGTT+TGG - chr8_3:52839892-52839911 Msa1294900:exon 30.0%
AATGATATATGGTTGTCCTT+GGG + chr8_3:52839988-52840007 None:intergenic 30.0%
AAAATGAGGTTGGAAGAAAT+GGG - chr8_3:52840173-52840192 Msa1294900:intron 30.0%
TACAGACAAAAAAAGGAAGT+TGG + chr8_3:52840491-52840510 None:intergenic 30.0%
CTGATCTTACAGACAAAAAA+AGG + chr8_3:52840498-52840517 None:intergenic 30.0%
!!! CTGTTATTTTCTTCATTCGT+AGG + chr8_3:52840699-52840718 None:intergenic 30.0%
CTGAATTGTATTGTTTGACA+AGG - chr8_3:52840724-52840743 Msa1294900:exon 30.0%
! ATTTTCCAATATTCTCCCTT+AGG + chr8_3:52839596-52839615 None:intergenic 30.0%
TAAGGGAGAATATTGGAAAA+TGG - chr8_3:52839595-52839614 Msa1294900:three_prime_UTR 30.0%
AAGGGAGAATATTGGAAAAT+GGG - chr8_3:52839596-52839615 Msa1294900:three_prime_UTR 30.0%
!! GAGGTTTTATGAAGAAATCT+TGG - chr8_3:52839709-52839728 Msa1294900:three_prime_UTR 30.0%
! TATGAAGAAATCTTGGGTTT+TGG - chr8_3:52839716-52839735 Msa1294900:three_prime_UTR 30.0%
CATTGAATTTGAAAGAGGAA+GGG + chr8_3:52839754-52839773 None:intergenic 30.0%
GGTAATGAAGCAAAGTATAT+AGG + chr8_3:52839791-52839810 None:intergenic 30.0%
AGGTTGTATAATTATGGGTT+TGG - chr8_3:52839892-52839911 Msa1294900:exon 30.0%
AATGATATATGGTTGTCCTT+GGG + chr8_3:52839988-52840007 None:intergenic 30.0%
AAAATGAGGTTGGAAGAAAT+GGG - chr8_3:52840173-52840192 Msa1294900:intron 30.0%
TACAGACAAAAAAAGGAAGT+TGG + chr8_3:52840491-52840510 None:intergenic 30.0%
CTGATCTTACAGACAAAAAA+AGG + chr8_3:52840498-52840517 None:intergenic 30.0%
!!! CTGTTATTTTCTTCATTCGT+AGG + chr8_3:52840699-52840718 None:intergenic 30.0%
CTGAATTGTATTGTTTGACA+AGG - chr8_3:52840724-52840743 Msa1294900:exon 30.0%
AGGGAGAATATTGGAAAATG+GGG - chr8_3:52839597-52839616 Msa1294900:three_prime_UTR 35.0%
ATATTGGAAAATGGGGATTG+AGG - chr8_3:52839604-52839623 Msa1294900:three_prime_UTR 35.0%
AAAATGGGGATTGAGGAAAT+TGG - chr8_3:52839611-52839630 Msa1294900:three_prime_UTR 35.0%
CAACACTGATCTGCATAATA+TGG + chr8_3:52839681-52839700 None:intergenic 35.0%
CATATTATGCAGATCAGTGT+TGG - chr8_3:52839679-52839698 Msa1294900:three_prime_UTR 35.0%
CCATTGAATTTGAAAGAGGA+AGG + chr8_3:52839755-52839774 None:intergenic 35.0%
CCTTCCTCTTTCAAATTCAA+TGG - chr8_3:52839752-52839771 Msa1294900:three_prime_UTR 35.0%
TGCTCCATTGAATTTGAAAG+AGG + chr8_3:52839759-52839778 None:intergenic 35.0%
CTTTCAAATTCAATGGAGCA+TGG - chr8_3:52839759-52839778 Msa1294900:three_prime_UTR 35.0%
CATAATTTGGATTCTCAAGC+AGG + chr8_3:52839924-52839943 None:intergenic 35.0%
GAATGATATATGGTTGTCCT+TGG + chr8_3:52839989-52840008 None:intergenic 35.0%
GAATGCACAGGTTAATTACT+TGG + chr8_3:52840011-52840030 None:intergenic 35.0%
ACTAGAAATTCAGAATGCAC+AGG + chr8_3:52840023-52840042 None:intergenic 35.0%
GAAAATGAGGTTGGAAGAAA+TGG - chr8_3:52840172-52840191 Msa1294900:intron 35.0%
AAATGAGGTTGGAAGAAATG+GGG - chr8_3:52840174-52840193 Msa1294900:intron 35.0%
AATGGGGATGAAGTATGTTA+CGG - chr8_3:52840190-52840209 Msa1294900:intron 35.0%
GTTCTATCATGTATCCATCA+GGG + chr8_3:52840265-52840284 None:intergenic 35.0%
AGTTCTATCATGTATCCATC+AGG + chr8_3:52840266-52840285 None:intergenic 35.0%
CAGAAGAAATGGGAACTATT+GGG + chr8_3:52840307-52840326 None:intergenic 35.0%
AAACAGTCTTCTTAAAGCTC+TGG + chr8_3:52840355-52840374 None:intergenic 35.0%
!! AAGACTGTTTCTAACAAGTG+TGG - chr8_3:52840365-52840384 Msa1294900:CDS 35.0%
TTCTAACAAGTGTGGATTCA+TGG - chr8_3:52840373-52840392 Msa1294900:CDS 35.0%
AGGGAGAATATTGGAAAATG+GGG - chr8_3:52839597-52839616 Msa1294900:three_prime_UTR 35.0%
ATATTGGAAAATGGGGATTG+AGG - chr8_3:52839604-52839623 Msa1294900:three_prime_UTR 35.0%
AAAATGGGGATTGAGGAAAT+TGG - chr8_3:52839611-52839630 Msa1294900:three_prime_UTR 35.0%
CAACACTGATCTGCATAATA+TGG + chr8_3:52839681-52839700 None:intergenic 35.0%
CATATTATGCAGATCAGTGT+TGG - chr8_3:52839679-52839698 Msa1294900:three_prime_UTR 35.0%
CCATTGAATTTGAAAGAGGA+AGG + chr8_3:52839755-52839774 None:intergenic 35.0%
CCTTCCTCTTTCAAATTCAA+TGG - chr8_3:52839752-52839771 Msa1294900:three_prime_UTR 35.0%
TGCTCCATTGAATTTGAAAG+AGG + chr8_3:52839759-52839778 None:intergenic 35.0%
CTTTCAAATTCAATGGAGCA+TGG - chr8_3:52839759-52839778 Msa1294900:three_prime_UTR 35.0%
CATAATTTGGATTCTCAAGC+AGG + chr8_3:52839924-52839943 None:intergenic 35.0%
GAATGATATATGGTTGTCCT+TGG + chr8_3:52839989-52840008 None:intergenic 35.0%
GAATGCACAGGTTAATTACT+TGG + chr8_3:52840011-52840030 None:intergenic 35.0%
ACTAGAAATTCAGAATGCAC+AGG + chr8_3:52840023-52840042 None:intergenic 35.0%
GAAAATGAGGTTGGAAGAAA+TGG - chr8_3:52840172-52840191 Msa1294900:intron 35.0%
AAATGAGGTTGGAAGAAATG+GGG - chr8_3:52840174-52840193 Msa1294900:intron 35.0%
AATGGGGATGAAGTATGTTA+CGG - chr8_3:52840190-52840209 Msa1294900:intron 35.0%
GTTCTATCATGTATCCATCA+GGG + chr8_3:52840265-52840284 None:intergenic 35.0%
AGTTCTATCATGTATCCATC+AGG + chr8_3:52840266-52840285 None:intergenic 35.0%
CAGAAGAAATGGGAACTATT+GGG + chr8_3:52840307-52840326 None:intergenic 35.0%
AAACAGTCTTCTTAAAGCTC+TGG + chr8_3:52840355-52840374 None:intergenic 35.0%
!! AAGACTGTTTCTAACAAGTG+TGG - chr8_3:52840365-52840384 Msa1294900:CDS 35.0%
TTCTAACAAGTGTGGATTCA+TGG - chr8_3:52840373-52840392 Msa1294900:CDS 35.0%
!! TGAGTGAAGAAGTGTCCTAA+GGG - chr8_3:52839578-52839597 Msa1294900:three_prime_UTR 40.0%
AGTGTCCTAAGGGAGAATAT+TGG - chr8_3:52839588-52839607 Msa1294900:three_prime_UTR 40.0%
ACATGATTCAGTGAGAGAAG+GGG + chr8_3:52839656-52839675 None:intergenic 40.0%
CACATGATTCAGTGAGAGAA+GGG + chr8_3:52839657-52839676 None:intergenic 40.0%
ACACATGATTCAGTGAGAGA+AGG + chr8_3:52839658-52839677 None:intergenic 40.0%
TGGTTGTCCTTGGGATTAAT+GGG + chr8_3:52839979-52839998 None:intergenic 40.0%
ATGGTTGTCCTTGGGATTAA+TGG + chr8_3:52839980-52839999 None:intergenic 40.0%
TTTGTGCAGTGTACAGATGT+TGG - chr8_3:52840143-52840162 Msa1294900:CDS 40.0%
TTGTGCAGTGTACAGATGTT+GGG - chr8_3:52840144-52840163 Msa1294900:CDS 40.0%
!! ATGTTGGGCTAGTGAAAATG+AGG - chr8_3:52840159-52840178 Msa1294900:CDS 40.0%
GATGAAGTATGTTACGGCTT+TGG - chr8_3:52840196-52840215 Msa1294900:intron 40.0%
GCAGAAGAAATGGGAACTAT+TGG + chr8_3:52840308-52840327 None:intergenic 40.0%
! TACGTATTAGCTTGGAAGTG+TGG + chr8_3:52839543-52839562 None:intergenic 40.0%
!! TGAGTGAAGAAGTGTCCTAA+GGG - chr8_3:52839578-52839597 Msa1294900:three_prime_UTR 40.0%
AGTGTCCTAAGGGAGAATAT+TGG - chr8_3:52839588-52839607 Msa1294900:three_prime_UTR 40.0%
ACATGATTCAGTGAGAGAAG+GGG + chr8_3:52839656-52839675 None:intergenic 40.0%
CACATGATTCAGTGAGAGAA+GGG + chr8_3:52839657-52839676 None:intergenic 40.0%
ACACATGATTCAGTGAGAGA+AGG + chr8_3:52839658-52839677 None:intergenic 40.0%
TGGTTGTCCTTGGGATTAAT+GGG + chr8_3:52839979-52839998 None:intergenic 40.0%
ATGGTTGTCCTTGGGATTAA+TGG + chr8_3:52839980-52839999 None:intergenic 40.0%
TTTGTGCAGTGTACAGATGT+TGG - chr8_3:52840143-52840162 Msa1294900:CDS 40.0%
TTGTGCAGTGTACAGATGTT+GGG - chr8_3:52840144-52840163 Msa1294900:CDS 40.0%
!! ATGTTGGGCTAGTGAAAATG+AGG - chr8_3:52840159-52840178 Msa1294900:CDS 40.0%
GATGAAGTATGTTACGGCTT+TGG - chr8_3:52840196-52840215 Msa1294900:intron 40.0%
GCAGAAGAAATGGGAACTAT+TGG + chr8_3:52840308-52840327 None:intergenic 40.0%
!! CTGAGTGAAGAAGTGTCCTA+AGG - chr8_3:52839577-52839596 Msa1294900:three_prime_UTR 45.0%
TGGGTCGTGATTCACTCATA+GGG + chr8_3:52839960-52839979 None:intergenic 45.0%
ATGGGTCGTGATTCACTCAT+AGG + chr8_3:52839961-52839980 None:intergenic 45.0%
ATCACGACCCATTAATCCCA+AGG - chr8_3:52839969-52839988 Msa1294900:CDS 45.0%
!! TGGGCTAGTGAAAATGAGGT+TGG - chr8_3:52840163-52840182 Msa1294900:CDS 45.0%
! GTATGTTACGGCTTTGGTAG+AGG - chr8_3:52840202-52840221 Msa1294900:intron 45.0%
GGTAGAGGATGAAGGAATCA+AGG - chr8_3:52840217-52840236 Msa1294900:intron 45.0%
AGAGGATGAAGGAATCAAGG+TGG - chr8_3:52840220-52840239 Msa1294900:intron 45.0%
TCATGTATCCATCAGGGTCA+TGG + chr8_3:52840259-52840278 None:intergenic 45.0%
GAGCAGGAAGCAGAAGAAAT+GGG + chr8_3:52840317-52840336 None:intergenic 45.0%
TATGATGCGAAGCTTGAGCA+AGG - chr8_3:52840403-52840422 Msa1294900:CDS 45.0%
!! CTGAGTGAAGAAGTGTCCTA+AGG - chr8_3:52839577-52839596 Msa1294900:three_prime_UTR 45.0%
TGGGTCGTGATTCACTCATA+GGG + chr8_3:52839960-52839979 None:intergenic 45.0%
ATGGGTCGTGATTCACTCAT+AGG + chr8_3:52839961-52839980 None:intergenic 45.0%
ATCACGACCCATTAATCCCA+AGG - chr8_3:52839969-52839988 Msa1294900:CDS 45.0%
!! TGGGCTAGTGAAAATGAGGT+TGG - chr8_3:52840163-52840182 Msa1294900:CDS 45.0%
! GTATGTTACGGCTTTGGTAG+AGG - chr8_3:52840202-52840221 Msa1294900:intron 45.0%
GGTAGAGGATGAAGGAATCA+AGG - chr8_3:52840217-52840236 Msa1294900:intron 45.0%
AGAGGATGAAGGAATCAAGG+TGG - chr8_3:52840220-52840239 Msa1294900:intron 45.0%
TCATGTATCCATCAGGGTCA+TGG + chr8_3:52840259-52840278 None:intergenic 45.0%
GAGCAGGAAGCAGAAGAAAT+GGG + chr8_3:52840317-52840336 None:intergenic 45.0%
TATGATGCGAAGCTTGAGCA+AGG - chr8_3:52840403-52840422 Msa1294900:CDS 45.0%
TTCAGTGAGAGAAGGGGTAG+TGG + chr8_3:52839650-52839669 None:intergenic 50.0%
! GATCAGTGTTGGACTCTGTG+AGG - chr8_3:52839690-52839709 Msa1294900:three_prime_UTR 50.0%
GGGTCGTGATTCACTCATAG+GGG + chr8_3:52839959-52839978 None:intergenic 50.0%
! ACGGCTTTGGTAGAGGATGA+AGG - chr8_3:52840209-52840228 Msa1294900:intron 50.0%
TGAAGGAATCAAGGTGGAGC+AGG - chr8_3:52840226-52840245 Msa1294900:intron 50.0%
GTGTTCTTCCATGACCCTGA+TGG - chr8_3:52840248-52840267 Msa1294900:intron 50.0%
GGAGCAGGAAGCAGAAGAAA+TGG + chr8_3:52840318-52840337 None:intergenic 50.0%
TTCAGTGAGAGAAGGGGTAG+TGG + chr8_3:52839650-52839669 None:intergenic 50.0%
! GATCAGTGTTGGACTCTGTG+AGG - chr8_3:52839690-52839709 Msa1294900:three_prime_UTR 50.0%
GGGTCGTGATTCACTCATAG+GGG + chr8_3:52839959-52839978 None:intergenic 50.0%
! ACGGCTTTGGTAGAGGATGA+AGG - chr8_3:52840209-52840228 Msa1294900:intron 50.0%
TGAAGGAATCAAGGTGGAGC+AGG - chr8_3:52840226-52840245 Msa1294900:intron 50.0%
GTGTTCTTCCATGACCCTGA+TGG - chr8_3:52840248-52840267 Msa1294900:intron 50.0%
GGAGCAGGAAGCAGAAGAAA+TGG + chr8_3:52840318-52840337 None:intergenic 50.0%
TCCTGCTCCTTCAAGTCACG+AGG - chr8_3:52840329-52840348 Msa1294900:intron 55.0%
TCCTGCTCCTTCAAGTCACG+AGG - chr8_3:52840329-52840348 Msa1294900:intron 55.0%
GCCTCGTGACTTGAAGGAGC+AGG + chr8_3:52840333-52840352 None:intergenic 60.0%
GCTCTGGCCTCGTGACTTGA+AGG + chr8_3:52840339-52840358 None:intergenic 60.0%
GCCTCGTGACTTGAAGGAGC+AGG + chr8_3:52840333-52840352 None:intergenic 60.0%
GCTCTGGCCTCGTGACTTGA+AGG + chr8_3:52840339-52840358 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 52839543 52840798 52839543 ID=Msa1294900;Name=Msa1294900
chr8_3 mRNA 52839543 52840798 52839543 ID=Msa1294900-mRNA-1;Parent=Msa1294900;Name=Msa1294900-mRNA-1;_AED=0.06;_eAED=0.06;_QI=71|1|1|1|1|1|3|350|193
chr8_3 exon 52839543 52840189 52839543 ID=Msa1294900-mRNA-1:exon:15060;Parent=Msa1294900-mRNA-1
chr8_3 exon 52840330 52840447 52840330 ID=Msa1294900-mRNA-1:exon:15059;Parent=Msa1294900-mRNA-1
chr8_3 exon 52840561 52840798 52840561 ID=Msa1294900-mRNA-1:exon:15058;Parent=Msa1294900-mRNA-1
chr8_3 five_prime_UTR 52840728 52840798 52840728 ID=Msa1294900-mRNA-1:five_prime_utr;Parent=Msa1294900-mRNA-1
chr8_3 CDS 52840561 52840727 52840561 ID=Msa1294900-mRNA-1:cds;Parent=Msa1294900-mRNA-1
chr8_3 CDS 52840330 52840447 52840330 ID=Msa1294900-mRNA-1:cds;Parent=Msa1294900-mRNA-1
chr8_3 CDS 52839893 52840189 52839893 ID=Msa1294900-mRNA-1:cds;Parent=Msa1294900-mRNA-1
chr8_3 three_prime_UTR 52839543 52839892 52839543 ID=Msa1294900-mRNA-1:three_prime_utr;Parent=Msa1294900-mRNA-1
Gene Sequence

>Msa1294900

ATGGGGATTGAGGAAATTGGCAGCTATGAAGCACCACTACCCCTTCTCTCACTGAATCATGTGTCCATATTATGCAGATCAGTGTTGGACTCTGTGAGGTTTTATGAAGAAATCTTGGGTTTTGGTCTCATCAAACGCCCTTCCTCTTTCAAATTCAATGGAGCATGGTTGTATAATTATGGGTTTGGAATACACCTGCTTGAGAATCCAAATTATGATGAATTTGATGCCCCTATGAGTGAATCACGACCCATTAATCCCAAGGACAACCATATATCATTCCAATGTACAGATGTTGGGCTAGTGAAAATGAGGTTGGAAGAAATGGGGATGAAGTATGTTACGGCTTTGGTAGAGGATGAAGGAATCAAGGTGGAGCAGGTGTTCTTCCATGACCCTGATGGATACATGATAGAACTTTGCAACTGTGAGAATATCCCAATAGTTCCCATTTCTTCTGCTTCCTGCTCCTTCAAGTCACGAGGCCAGAGCTTTAAGAAGACTGTTTCTAACAAGTGTGGATTCATGGAAAATGTTATGATGCGAAGCTTGAGCAAGGACATGATGAACTTTGCATTTTAA
Protein sequence

>Msa1294900

MGIEEIGSYEAPLPLLSLNHVSILCRSVLDSVRFYEEILGFGLIKRPSSFKFNGAWLYNYGFGIHLLENPNYDEFDAPMSESRPINPKDNHISFQCTDVGLVKMRLEEMGMKYVTALVEDEGIKVEQVFFHDPDGYMIELCNCENIPIVPISSASCSFKSRGQSFKKTVSNKCGFMENVMMRSLSKDMMNFAF*