AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1343210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1343210 MtrunA17_Chr2g0280221 95.067 223 11 0 1 223 486 708 6.91e-150 433
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATTACGTTGCATCATTT+TGG 0.140284 tig0006328:-25724 None:intergenic
ACATGGAGACTATGAAATTT+TGG 0.242997 tig0006328:-26019 None:intergenic
TCTTAAATTTCCAACCTTAG+AGG 0.311423 tig0006328:-25753 None:intergenic
TCATATCTAAGTTGGTTTCC+TGG 0.321011 tig0006328:-26059 None:intergenic
CGTGGCTTTGTTGTTTCCTT+TGG 0.324727 tig0006328:-25941 None:intergenic
TTTAGCAGTGATTTCTTTCA+AGG 0.356949 tig0006328:-25249 None:intergenic
TTCCTGGTGGTGCAGCTCTT+AGG 0.360501 tig0006328:-26043 None:intergenic
GTAATGTTGTAATGCCTCTA+AGG 0.361643 tig0006328:+25739 Msa1343210:CDS
CTTAAACGGCTTAGGATTTG+TGG 0.393734 tig0006328:-25071 None:intergenic
CTTGCAACAGCTTGTAAACT+AGG 0.395460 tig0006328:+25884 Msa1343210:CDS
ACATCAGTTCTGAACTTAAA+CGG 0.420367 tig0006328:-25085 None:intergenic
TTAGAACCAGGATTTCTGTT+AGG 0.420665 tig0006328:-25657 None:intergenic
TACTACATTCATATCTAAGT+TGG 0.426218 tig0006328:-26067 None:intergenic
AAATCTACATCGACAACAAC+TGG 0.434150 tig0006328:+25016 Msa1343210:CDS
ACTTCAGAAACATGTTGATC+TGG 0.443215 tig0006328:+25680 Msa1343210:CDS
TTAAACGGCTTAGGATTTGT+GGG 0.455528 tig0006328:-25070 None:intergenic
GTTCTGAACTTAAACGGCTT+AGG 0.465133 tig0006328:-25079 None:intergenic
AAAGAAATCACTGCTAAAGA+TGG 0.476812 tig0006328:+25254 Msa1343210:CDS
AATTCTCAAAGAAGCAAAGC+TGG 0.494890 tig0006328:+25208 Msa1343210:CDS
TATATGAATGAAGCTAATAC+AGG 0.516332 tig0006328:-26208 None:intergenic
ATGCATCTTGTTCGCAAGGA+AGG 0.532188 tig0006328:+25972 Msa1343210:CDS
TGCCTAAGAGCTGCACCACC+AGG 0.532780 tig0006328:+26041 Msa1343210:CDS
AAAACACCTAACAGAAATCC+TGG 0.537750 tig0006328:+25651 Msa1343210:CDS
GATATGAATGTAGTAGAGCT+TGG 0.545897 tig0006328:+26075 Msa1343210:exon
AATGATGCATCTTGTTCGCA+AGG 0.550245 tig0006328:+25968 Msa1343210:CDS
TTAGCAGTGATTTCTTTCAA+GGG 0.566621 tig0006328:-25248 None:intergenic
TGTTTCTGAAGTTTAGAACC+AGG 0.570136 tig0006328:-25669 None:intergenic
ATTGTAATGCTGCAGGAAAG+GGG 0.604562 tig0006328:+25185 Msa1343210:intron
TGGTGCAGCTCTTAGGCACA+TGG 0.613861 tig0006328:-26036 None:intergenic
TTCAGACACTGTAAAACCAA+AGG 0.617882 tig0006328:+25925 Msa1343210:CDS
GAGAACTTTGACTGTCCCAA+AGG 0.620794 tig0006328:+25994 Msa1343210:CDS
TATCTAAGTTGGTTTCCTGG+TGG 0.622856 tig0006328:-26056 None:intergenic
ATTAGCTTCATTCATATACG+AGG 0.669464 tig0006328:+26213 Msa1343210:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCAATCTATTATAATA+TGG + tig0006328:25788-25807 Msa1343210:intron 10.0%
!!! ATTTTCAATCTATTATAATA+TGG + tig0006328:25788-25807 Msa1343210:intron 10.0%
!! ATACAAAAACAAATAATGAA+AGG - tig0006328:25340-25359 None:intergenic 15.0%
!!! TTGCTTAATATGTTTTTTAA+AGG + tig0006328:25538-25557 Msa1343210:intron 15.0%
!! AAAACGAAAAACATTTAATT+TGG + tig0006328:26274-26293 Msa1343210:three_prime_UTR 15.0%
!! ATACAAAAACAAATAATGAA+AGG - tig0006328:25340-25359 None:intergenic 15.0%
!!! TTGCTTAATATGTTTTTTAA+AGG + tig0006328:25538-25557 Msa1343210:intron 15.0%
!! AAAACGAAAAACATTTAATT+TGG + tig0006328:26274-26293 Msa1343210:three_prime_UTR 15.0%
!! ATCTATTATAATATGGTCTT+TGG + tig0006328:25795-25814 Msa1343210:intron 20.0%
!! ATCTATTATAATATGGTCTT+TGG + tig0006328:25795-25814 Msa1343210:intron 20.0%
!! AAATAATGAAAGGTTGATTG+AGG - tig0006328:25330-25349 None:intergenic 25.0%
!!! TTTGATTTTGTTGAGTTGTT+CGG - tig0006328:25464-25483 None:intergenic 25.0%
!!! AATATGTTTTTTAAAGGCAG+TGG + tig0006328:25544-25563 Msa1343210:intron 25.0%
!!! TATAATATGGTCTTTGGAAT+TGG + tig0006328:25801-25820 Msa1343210:intron 25.0%
!!! ATAATATGGTCTTTGGAATT+GGG + tig0006328:25802-25821 Msa1343210:intron 25.0%
!!! TATGAAATTTTGGTTCCTTT+GGG - tig0006328:26012-26031 None:intergenic 25.0%
! TACTACATTCATATCTAAGT+TGG - tig0006328:26070-26089 None:intergenic 25.0%
! AAAAAATTCATCTATCTAGC+AGG - tig0006328:26177-26196 None:intergenic 25.0%
! TATATGAATGAAGCTAATAC+AGG - tig0006328:26211-26230 None:intergenic 25.0%
!! AAATAATGAAAGGTTGATTG+AGG - tig0006328:25330-25349 None:intergenic 25.0%
!!! TTTGATTTTGTTGAGTTGTT+CGG - tig0006328:25464-25483 None:intergenic 25.0%
!!! AATATGTTTTTTAAAGGCAG+TGG + tig0006328:25544-25563 Msa1343210:intron 25.0%
!!! TATAATATGGTCTTTGGAAT+TGG + tig0006328:25801-25820 Msa1343210:intron 25.0%
!!! ATAATATGGTCTTTGGAATT+GGG + tig0006328:25802-25821 Msa1343210:intron 25.0%
!!! TATGAAATTTTGGTTCCTTT+GGG - tig0006328:26012-26031 None:intergenic 25.0%
! TACTACATTCATATCTAAGT+TGG - tig0006328:26070-26089 None:intergenic 25.0%
! AAAAAATTCATCTATCTAGC+AGG - tig0006328:26177-26196 None:intergenic 25.0%
! TATATGAATGAAGCTAATAC+AGG - tig0006328:26211-26230 None:intergenic 25.0%
!! ACATCAGTTCTGAACTTAAA+CGG - tig0006328:25088-25107 None:intergenic 30.0%
TTCAGAACTGATGTAAGTAA+GGG + tig0006328:25094-25113 Msa1343210:intron 30.0%
ATGATATATTGTAATGCTGC+AGG + tig0006328:25178-25197 Msa1343210:intron 30.0%
TTAGCAGTGATTTCTTTCAA+GGG - tig0006328:25251-25270 None:intergenic 30.0%
TTTAGCAGTGATTTCTTTCA+AGG - tig0006328:25252-25271 None:intergenic 30.0%
AAAGAAATCACTGCTAAAGA+TGG + tig0006328:25254-25273 Msa1343210:CDS 30.0%
ACAAGTTTCATTCTTCTGAA+AGG - tig0006328:25391-25410 None:intergenic 30.0%
ACTGTAAGTTAATGCACATA+AGG + tig0006328:25483-25502 Msa1343210:intron 30.0%
TATTAAGCAAGCAGTAATTC+AGG - tig0006328:25528-25547 None:intergenic 30.0%
! ACTTCCTCAAAATTTTGTGT+AGG + tig0006328:25572-25591 Msa1343210:intron 30.0%
! CTTCCTCAAAATTTTGTGTA+GGG + tig0006328:25573-25592 Msa1343210:intron 30.0%
! TTTCCCTACACAAAATTTTG+AGG - tig0006328:25579-25598 None:intergenic 30.0%
! AACATTACGTTGCATCATTT+TGG - tig0006328:25727-25746 None:intergenic 30.0%
TCTTAAATTTCCAACCTTAG+AGG - tig0006328:25756-25775 None:intergenic 30.0%
!!! CTATGAAATTTTGGTTCCTT+TGG - tig0006328:26013-26032 None:intergenic 30.0%
! ACATGGAGACTATGAAATTT+TGG - tig0006328:26022-26041 None:intergenic 30.0%
! GATCATTGAATTTTGTTGAG+TGG + tig0006328:26111-26130 Msa1343210:three_prime_UTR 30.0%
! TGAATTTTGTTGAGTGGTTT+TGG + tig0006328:26117-26136 Msa1343210:three_prime_UTR 30.0%
! ATTAGCTTCATTCATATACG+AGG + tig0006328:26213-26232 Msa1343210:three_prime_UTR 30.0%
!! ACATCAGTTCTGAACTTAAA+CGG - tig0006328:25088-25107 None:intergenic 30.0%
TTCAGAACTGATGTAAGTAA+GGG + tig0006328:25094-25113 Msa1343210:intron 30.0%
ATGATATATTGTAATGCTGC+AGG + tig0006328:25178-25197 Msa1343210:intron 30.0%
TTAGCAGTGATTTCTTTCAA+GGG - tig0006328:25251-25270 None:intergenic 30.0%
TTTAGCAGTGATTTCTTTCA+AGG - tig0006328:25252-25271 None:intergenic 30.0%
AAAGAAATCACTGCTAAAGA+TGG + tig0006328:25254-25273 Msa1343210:CDS 30.0%
ACAAGTTTCATTCTTCTGAA+AGG - tig0006328:25391-25410 None:intergenic 30.0%
ACTGTAAGTTAATGCACATA+AGG + tig0006328:25483-25502 Msa1343210:intron 30.0%
TATTAAGCAAGCAGTAATTC+AGG - tig0006328:25528-25547 None:intergenic 30.0%
! ACTTCCTCAAAATTTTGTGT+AGG + tig0006328:25572-25591 Msa1343210:intron 30.0%
! CTTCCTCAAAATTTTGTGTA+GGG + tig0006328:25573-25592 Msa1343210:intron 30.0%
! TTTCCCTACACAAAATTTTG+AGG - tig0006328:25579-25598 None:intergenic 30.0%
! AACATTACGTTGCATCATTT+TGG - tig0006328:25727-25746 None:intergenic 30.0%
TCTTAAATTTCCAACCTTAG+AGG - tig0006328:25756-25775 None:intergenic 30.0%
!!! CTATGAAATTTTGGTTCCTT+TGG - tig0006328:26013-26032 None:intergenic 30.0%
! ACATGGAGACTATGAAATTT+TGG - tig0006328:26022-26041 None:intergenic 30.0%
! GATCATTGAATTTTGTTGAG+TGG + tig0006328:26111-26130 Msa1343210:three_prime_UTR 30.0%
! TGAATTTTGTTGAGTGGTTT+TGG + tig0006328:26117-26136 Msa1343210:three_prime_UTR 30.0%
! ATTAGCTTCATTCATATACG+AGG + tig0006328:26213-26232 Msa1343210:three_prime_UTR 30.0%
AAATCTACATCGACAACAAC+TGG + tig0006328:25016-25035 Msa1343210:CDS 35.0%
TTAAACGGCTTAGGATTTGT+GGG - tig0006328:25073-25092 None:intergenic 35.0%
GTTCAGAACTGATGTAAGTA+AGG + tig0006328:25093-25112 Msa1343210:intron 35.0%
ATATTGTAATGCTGCAGGAA+AGG + tig0006328:25183-25202 Msa1343210:intron 35.0%
TATTGTAATGCTGCAGGAAA+GGG + tig0006328:25184-25203 Msa1343210:intron 35.0%
AATTCTCAAAGAAGCAAAGC+TGG + tig0006328:25208-25227 Msa1343210:CDS 35.0%
AAAACACCTAACAGAAATCC+TGG + tig0006328:25651-25670 Msa1343210:CDS 35.0%
TTAGAACCAGGATTTCTGTT+AGG - tig0006328:25660-25679 None:intergenic 35.0%
!! TGTTTCTGAAGTTTAGAACC+AGG - tig0006328:25672-25691 None:intergenic 35.0%
ACTTCAGAAACATGTTGATC+TGG + tig0006328:25680-25699 Msa1343210:CDS 35.0%
GTAATGTTGTAATGCCTCTA+AGG + tig0006328:25739-25758 Msa1343210:CDS 35.0%
TTCAGACACTGTAAAACCAA+AGG + tig0006328:25925-25944 Msa1343210:CDS 35.0%
!! CAAGATGCATCATTTTTACG+TGG - tig0006328:25962-25981 None:intergenic 35.0%
!! TCATATCTAAGTTGGTTTCC+TGG - tig0006328:26062-26081 None:intergenic 35.0%
GATATGAATGTAGTAGAGCT+TGG + tig0006328:26075-26094 Msa1343210:exon 35.0%
AGTTGTTGTCGATGTAGATT+TGG - tig0006328:25017-25036 None:intergenic 35.0%
AAATCTACATCGACAACAAC+TGG + tig0006328:25016-25035 Msa1343210:CDS 35.0%
TTAAACGGCTTAGGATTTGT+GGG - tig0006328:25073-25092 None:intergenic 35.0%
GTTCAGAACTGATGTAAGTA+AGG + tig0006328:25093-25112 Msa1343210:intron 35.0%
ATATTGTAATGCTGCAGGAA+AGG + tig0006328:25183-25202 Msa1343210:intron 35.0%
TATTGTAATGCTGCAGGAAA+GGG + tig0006328:25184-25203 Msa1343210:intron 35.0%
AATTCTCAAAGAAGCAAAGC+TGG + tig0006328:25208-25227 Msa1343210:CDS 35.0%
AAAACACCTAACAGAAATCC+TGG + tig0006328:25651-25670 Msa1343210:CDS 35.0%
TTAGAACCAGGATTTCTGTT+AGG - tig0006328:25660-25679 None:intergenic 35.0%
!! TGTTTCTGAAGTTTAGAACC+AGG - tig0006328:25672-25691 None:intergenic 35.0%
ACTTCAGAAACATGTTGATC+TGG + tig0006328:25680-25699 Msa1343210:CDS 35.0%
GTAATGTTGTAATGCCTCTA+AGG + tig0006328:25739-25758 Msa1343210:CDS 35.0%
TTCAGACACTGTAAAACCAA+AGG + tig0006328:25925-25944 Msa1343210:CDS 35.0%
!! CAAGATGCATCATTTTTACG+TGG - tig0006328:25962-25981 None:intergenic 35.0%
!! TCATATCTAAGTTGGTTTCC+TGG - tig0006328:26062-26081 None:intergenic 35.0%
GATATGAATGTAGTAGAGCT+TGG + tig0006328:26075-26094 Msa1343210:exon 35.0%
CTTAAACGGCTTAGGATTTG+TGG - tig0006328:25074-25093 None:intergenic 40.0%
! GTTCTGAACTTAAACGGCTT+AGG - tig0006328:25082-25101 None:intergenic 40.0%
ATTGTAATGCTGCAGGAAAG+GGG + tig0006328:25185-25204 Msa1343210:intron 40.0%
!! TGTTGTAATGCCTCTAAGGT+TGG + tig0006328:25743-25762 Msa1343210:intron 40.0%
CTTGCAACAGCTTGTAAACT+AGG + tig0006328:25884-25903 Msa1343210:CDS 40.0%
AATGATGCATCTTGTTCGCA+AGG + tig0006328:25968-25987 Msa1343210:CDS 40.0%
!! TATCTAAGTTGGTTTCCTGG+TGG - tig0006328:26059-26078 None:intergenic 40.0%
CTTAAACGGCTTAGGATTTG+TGG - tig0006328:25074-25093 None:intergenic 40.0%
! GTTCTGAACTTAAACGGCTT+AGG - tig0006328:25082-25101 None:intergenic 40.0%
ATTGTAATGCTGCAGGAAAG+GGG + tig0006328:25185-25204 Msa1343210:intron 40.0%
!! TGTTGTAATGCCTCTAAGGT+TGG + tig0006328:25743-25762 Msa1343210:intron 40.0%
CTTGCAACAGCTTGTAAACT+AGG + tig0006328:25884-25903 Msa1343210:CDS 40.0%
AATGATGCATCTTGTTCGCA+AGG + tig0006328:25968-25987 Msa1343210:CDS 40.0%
!! TATCTAAGTTGGTTTCCTGG+TGG - tig0006328:26059-26078 None:intergenic 40.0%
GTAAGGGCTTCTGTTCTCAT+TGG + tig0006328:25110-25129 Msa1343210:intron 45.0%
TCAGGTGCTTAGCAATAAGC+AGG - tig0006328:25510-25529 None:intergenic 45.0%
!! CGTGGCTTTGTTGTTTCCTT+TGG - tig0006328:25944-25963 None:intergenic 45.0%
ATGCATCTTGTTCGCAAGGA+AGG + tig0006328:25972-25991 Msa1343210:CDS 45.0%
GAGAACTTTGACTGTCCCAA+AGG + tig0006328:25994-26013 Msa1343210:CDS 45.0%
GTAAGGGCTTCTGTTCTCAT+TGG + tig0006328:25110-25129 Msa1343210:intron 45.0%
TCAGGTGCTTAGCAATAAGC+AGG - tig0006328:25510-25529 None:intergenic 45.0%
!! CGTGGCTTTGTTGTTTCCTT+TGG - tig0006328:25944-25963 None:intergenic 45.0%
ATGCATCTTGTTCGCAAGGA+AGG + tig0006328:25972-25991 Msa1343210:CDS 45.0%
GAGAACTTTGACTGTCCCAA+AGG + tig0006328:25994-26013 Msa1343210:CDS 45.0%
TGGTGCAGCTCTTAGGCACA+TGG - tig0006328:26039-26058 None:intergenic 55.0%
! TTCCTGGTGGTGCAGCTCTT+AGG - tig0006328:26046-26065 None:intergenic 55.0%
TGGTGCAGCTCTTAGGCACA+TGG - tig0006328:26039-26058 None:intergenic 55.0%
! TTCCTGGTGGTGCAGCTCTT+AGG - tig0006328:26046-26065 None:intergenic 55.0%
TGCCTAAGAGCTGCACCACC+AGG + tig0006328:26041-26060 Msa1343210:CDS 60.0%
TGCCTAAGAGCTGCACCACC+AGG + tig0006328:26041-26060 Msa1343210:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0006328 gene 25016 26301 25016 ID=Msa1343210;Name=Msa1343210
tig0006328 mRNA 25016 26301 25016 ID=Msa1343210-mRNA-1;Parent=Msa1343210;Name=Msa1343210-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.5|0.4|0.6|1|0.8|5|226|223
tig0006328 exon 25016 25105 25016 ID=Msa1343210-mRNA-1:exon:4024;Parent=Msa1343210-mRNA-1
tig0006328 exon 25200 25301 25200 ID=Msa1343210-mRNA-1:exon:4025;Parent=Msa1343210-mRNA-1
tig0006328 exon 25396 25485 25396 ID=Msa1343210-mRNA-1:exon:4026;Parent=Msa1343210-mRNA-1
tig0006328 exon 25594 25760 25594 ID=Msa1343210-mRNA-1:exon:4027;Parent=Msa1343210-mRNA-1
tig0006328 exon 25853 26301 25853 ID=Msa1343210-mRNA-1:exon:4028;Parent=Msa1343210-mRNA-1
tig0006328 CDS 25016 25105 25016 ID=Msa1343210-mRNA-1:cds;Parent=Msa1343210-mRNA-1
tig0006328 CDS 25200 25301 25200 ID=Msa1343210-mRNA-1:cds;Parent=Msa1343210-mRNA-1
tig0006328 CDS 25396 25485 25396 ID=Msa1343210-mRNA-1:cds;Parent=Msa1343210-mRNA-1
tig0006328 CDS 25594 25760 25594 ID=Msa1343210-mRNA-1:cds;Parent=Msa1343210-mRNA-1
tig0006328 CDS 25853 26075 25853 ID=Msa1343210-mRNA-1:cds;Parent=Msa1343210-mRNA-1
tig0006328 three_prime_UTR 26076 26301 26076 ID=Msa1343210-mRNA-1:three_prime_utr;Parent=Msa1343210-mRNA-1
Gene Sequence

>Msa1343210

AAATCTACATCGACAACAACTGGTTCTCAAGTTGTAAAATACAGAAAATTAAAGCCCACAAATCCTAAGCCGTTTAAGTTCAGAACTGATGAAAGGGGAATTCTCAAAGAAGCAAAGCTGGAGAAAAAAATCACATCACCCTTGAAAGAAATCACTGCTAAAGATGGCAAAGCAATAAAGAAACATAAGAATAAGAATGAAACTTGTACAGCACAAAGTGATCAAGACAATTATAGCAGTTGCAGCGAAAATTCAAACCGAACAACTCAACAAAATCAAACTGGAAACATTCATAGTGACAACAACTACAACAGCAAAGTGCAACTTAAATTATCTGCTAAAACACCTAACAGAAATCCTGGTTCTAAACTTCAGAAACATGTTGATCTGGATGAAAATTTCAATAGAAAATCCAAAATGATGCAACGTAATGTTGTAATGCCTCTAAGTGTTTTGTCAAAGAAGAAAGAAAAAGTAGTTCTTGCAACAGCTTGTAAACTAGGTGTCATTACTGAAAAGCATTCAGACACTGTAAAACCAAAGGAAACAACAAAGCCACGTAAAAATGATGCATCTTGTTCGCAAGGAAGGAGAACTTTGACTGTCCCAAAGGAACCAAAATTTCATAGTCTCCATGTGCCTAAGAGCTGCACCACCAGGAAACCAACTTAG

Protein sequence

>Msa1343210

KSTSTTTGSQVVKYRKLKPTNPKPFKFRTDERGILKEAKLEKKITSPLKEITAKDGKAIKKHKNKNETCTAQSDQDNYSSCSENSNRTTQQNQTGNIHSDNNYNSKVQLKLSAKTPNRNPGSKLQKHVDLDENFNRKSKMMQRNVVMPLSVLSKKKEKVVLATACKLGVITEKHSDTVKPKETTKPRKNDASCSQGRRTLTVPKEPKFHSLHVPKSCTTRKPT*