AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0121330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0121330 MtrunA17_Chr1g0180381 95.455 198 3 1 1 192 1 198 1.16e-136 398
Msa0121330 MtrunA17_Chr1g0180381 94.086 186 10 1 187 371 289 474 8.02e-121 357
Msa0121330 MtrunA17_Chr7g0264891 78.392 199 30 3 1 192 1 193 2.70e-96 294
Msa0121330 MtrunA17_Chr7g0264891 61.622 185 68 2 187 369 284 467 1.29e-68 223
Msa0121330 MtrunA17_Chr1g0210321 64.141 198 61 2 1 192 1 194 1.93e-86 270
Msa0121330 MtrunA17_Chr1g0210321 66.667 123 41 0 193 315 291 413 6.86e-53 182
Msa0121330 MtrunA17_Chr6g0458201 63.636 165 47 2 34 192 26 183 5.30e-69 222
Msa0121330 MtrunA17_Chr6g0458201 53.659 123 57 0 193 315 280 402 8.78e-40 145
Msa0121330 MtrunA17_Chr4g0058051 58.523 176 60 2 34 203 28 196 2.36e-66 215
Msa0121330 MtrunA17_Chr4g0058051 55.738 122 53 1 193 313 282 403 1.06e-36 137
Msa0121330 MtrunA17_Chr1g0169731 54.971 171 64 2 34 198 72 235 1.26e-60 201
Msa0121330 MtrunA17_Chr1g0169731 54.545 121 55 0 193 313 326 446 1.24e-37 140
Msa0121330 MtrunA17_Chr3g0116521 55.682 176 60 3 9 178 8 171 6.81e-58 193
Msa0121330 MtrunA17_Chr3g0116521 56.198 121 53 0 193 313 282 402 2.22e-38 142
Msa0121330 MtrunA17_Chr7g0237761 52.047 171 69 2 34 198 66 229 4.69e-56 189
Msa0121330 MtrunA17_Chr7g0237761 56.198 121 53 0 193 313 320 440 1.58e-37 140
Msa0121330 MtrunA17_Chr4g0060211 50.303 165 68 3 33 187 50 210 5.41e-51 176
Msa0121330 MtrunA17_Chr4g0060211 52.137 117 54 2 193 309 312 426 9.15e-33 127
Msa0121330 MtrunA17_Chr3g0123321 56.667 150 49 4 34 177 28 167 3.31e-50 173
Msa0121330 MtrunA17_Chr3g0123321 57.265 117 50 0 193 309 279 395 5.99e-34 129
Msa0121330 MtrunA17_Chr3g0123331 47.541 183 75 4 2 178 3 170 7.86e-49 169
Msa0121330 MtrunA17_Chr3g0123331 56.034 116 50 1 195 309 283 398 3.20e-38 141
Msa0121330 MtrunA17_Chr5g0416061 50.000 166 68 3 33 187 61 222 2.16e-48 169
Msa0121330 MtrunA17_Chr5g0416061 55.814 86 38 0 193 278 324 409 2.85e-28 114
Msa0121330 MtrunA17_Chr6g0452861 48.810 168 73 3 33 188 61 227 9.92e-46 162
Msa0121330 MtrunA17_Chr6g0452861 52.542 118 55 1 193 309 328 445 2.23e-33 129
Msa0121330 MtrunA17_Chr8g0362561 56.250 128 50 1 66 187 94 221 3.92e-45 160
Msa0121330 MtrunA17_Chr8g0362561 43.511 131 66 2 187 309 317 447 3.75e-28 114
Msa0121330 MtrunA17_Chr4g0075231 57.463 134 50 2 71 198 39 171 7.26e-44 155
Msa0121330 MtrunA17_Chr4g0075231 45.763 118 61 2 193 309 262 377 2.83e-27 111
Msa0121330 MtrunA17_Chr2g0288921 51.299 154 68 2 57 204 49 201 1.01e-43 156
Msa0121330 MtrunA17_Chr2g0288921 46.825 126 58 3 193 314 286 406 1.52e-26 109
Msa0121330 MtrunA17_Chr5g0416041 50.932 161 72 2 33 186 61 221 1.84e-42 153
Msa0121330 MtrunA17_Chr5g0416041 48.305 118 60 1 193 309 324 441 3.81e-30 120
Msa0121330 MtrunA17_Chr7g0216581 46.552 174 73 3 33 186 50 223 2.08e-39 145
Msa0121330 MtrunA17_Chr7g0216581 47.458 118 61 1 193 309 326 443 2.44e-31 123
Msa0121330 MtrunA17_Chr8g0373761 41.892 148 74 4 59 196 30 175 2.38e-28 114
Msa0121330 MtrunA17_Chr8g0373761 37.500 136 78 4 180 313 258 388 3.15e-17 82.4
Msa0121330 MtrunA17_Chr2g0288901 47.059 119 58 3 193 310 35 149 8.44e-28 107
Msa0121330 MtrunA17_Chr8g0373721 39.865 148 77 4 59 196 30 175 1.43e-26 109
Msa0121330 MtrunA17_Chr8g0373721 37.681 138 75 5 180 313 258 388 1.20e-15 77.8
Msa0121330 MtrunA17_Chr3g0089181 40.123 162 76 7 45 196 6 156 2.01e-26 108
Msa0121330 MtrunA17_Chr3g0089181 36.029 136 80 4 180 313 239 369 7.57e-15 75.1
Msa0121330 MtrunA17_Chr8g0373701 38.816 152 76 6 54 196 87 230 3.28e-24 103
Msa0121330 MtrunA17_Chr8g0373701 35.294 136 81 4 180 313 313 443 1.15e-13 72.0
Msa0121330 MtrunA17_Chr8g0373731 39.189 148 78 4 59 196 30 175 1.05e-19 89.7
Msa0121330 MtrunA17_Chr8g0373731 38.235 136 77 4 180 313 258 388 2.98e-16 79.3
Msa0121330 MtrunA17_Chr5g0403391 38.462 156 81 6 71 213 41 194 3.02e-19 88.2
Msa0121330 MtrunA17_Chr5g0403391 37.705 122 72 4 194 313 268 387 1.85e-13 71.2
Msa0121330 MtrunA17_Chr5g0403481 41.176 136 68 4 71 196 41 174 3.55e-19 88.2
Msa0121330 MtrunA17_Chr5g0403481 38.400 125 67 6 194 313 268 387 2.34e-13 70.9
Msa0121330 MtrunA17_Chr5g0403461 41.176 136 68 4 71 196 41 174 3.55e-19 88.2
Msa0121330 MtrunA17_Chr5g0403461 38.400 125 67 6 194 313 268 387 2.34e-13 70.9
Msa0121330 MtrunA17_Chr5g0403491 41.176 136 68 4 71 196 41 174 3.55e-19 88.2
Msa0121330 MtrunA17_Chr5g0403491 38.400 125 67 6 194 313 268 387 2.34e-13 70.9
Msa0121330 MtrunA17_Chr5g0414541 37.179 156 83 5 71 213 41 194 5.14e-19 87.8
Msa0121330 MtrunA17_Chr5g0414541 38.281 128 63 6 194 313 268 387 2.34e-13 70.9
Msa0121330 MtrunA17_Chr5g0403411 37.179 156 83 5 71 213 41 194 1.18e-17 83.6
Msa0121330 MtrunA17_Chr5g0403411 36.066 122 74 4 194 313 268 387 1.36e-12 68.6
Msa0121330 MtrunA17_Chr3g0113551 69.388 49 15 0 195 243 242 290 1.42e-17 82.4
Msa0121330 MtrunA17_Chr4g0031851 36.765 136 79 4 180 313 203 333 8.51e-16 77.8
Msa0121330 MtrunA17_Chr4g0031851 44.211 95 43 3 110 196 28 120 2.65e-15 76.6
Msa0121330 MtrunA17_Chr1g0183941 38.060 134 72 5 180 309 140 266 8.82e-16 76.6
Msa0121330 MtrunA17_Chr1g0183941 49.123 57 29 0 140 196 1 57 4.12e-11 63.2
Msa0121330 MtrunA17_Chr2g0288911 58.491 53 22 0 140 192 1 53 8.37e-14 67.8
Msa0121330 MtrunA17_Chr1g0212931 39.423 104 61 2 82 184 7 109 1.78e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0121330 AT3G53190.1 68.317 202 52 4 1 192 2 201 1.31e-78 249
Msa0121330 AT3G53190.1 55.495 182 76 3 187 365 292 471 7.06e-60 201
Msa0121330 AT5G04310.3 58.049 205 68 3 6 192 18 222 2.61e-71 230
Msa0121330 AT5G04310.3 67.769 121 39 0 193 313 319 439 4.95e-50 174
Msa0121330 AT3G54920.1 60.104 193 64 3 21 200 23 215 3.26e-71 231
Msa0121330 AT3G54920.1 66.935 124 40 1 193 315 295 418 1.17e-49 174
Msa0121330 AT5G04310.1 58.049 205 68 3 6 192 18 222 1.32e-70 230
Msa0121330 AT5G04310.1 60.690 145 55 1 193 335 319 463 1.33e-50 177
Msa0121330 AT5G04310.2 58.049 205 68 3 6 192 18 222 1.32e-70 230
Msa0121330 AT5G04310.2 60.690 145 55 1 193 335 319 463 1.33e-50 177
Msa0121330 AT1G67750.1 60.494 162 49 3 34 189 32 184 3.33e-63 207
Msa0121330 AT1G67750.1 56.410 117 51 0 193 309 284 400 5.73e-34 130
Msa0121330 AT5G63180.1 54.011 187 67 4 9 189 33 206 9.91e-63 207
Msa0121330 AT5G63180.1 54.472 123 54 1 193 313 306 428 1.35e-35 135
Msa0121330 AT4G13710.2 57.310 171 64 2 34 198 69 236 9.23e-62 205
Msa0121330 AT4G13710.2 57.851 121 51 0 193 313 327 447 8.71e-42 152
Msa0121330 AT4G13710.1 57.310 171 64 2 34 198 88 255 9.50e-62 205
Msa0121330 AT4G13710.1 57.851 121 51 0 193 313 346 466 8.12e-42 152
Msa0121330 AT1G04680.1 47.368 209 91 4 8 198 7 214 4.93e-60 200
Msa0121330 AT1G04680.1 56.198 121 53 0 193 313 305 425 1.04e-38 143
Msa0121330 AT3G07010.1 53.216 171 67 2 34 198 38 201 1.83e-58 195
Msa0121330 AT3G07010.1 55.556 117 52 0 193 309 292 408 1.69e-36 137
Msa0121330 AT3G24670.1 54.971 171 64 2 34 198 62 225 2.21e-58 196
Msa0121330 AT3G24670.1 56.410 117 51 0 193 309 316 432 2.07e-35 134
Msa0121330 AT4G24780.2 57.233 159 56 2 26 178 21 173 1.16e-57 193
Msa0121330 AT4G24780.2 56.410 117 51 0 193 309 284 400 1.74e-34 131
Msa0121330 AT4G24780.1 57.233 159 56 2 26 178 21 173 1.16e-57 193
Msa0121330 AT4G24780.1 56.410 117 51 0 193 309 284 400 1.74e-34 131
Msa0121330 AT3G07010.2 60.432 139 49 1 66 198 30 168 2.03e-57 192
Msa0121330 AT3G07010.2 55.556 117 52 0 193 309 259 375 1.18e-36 137
Msa0121330 AT4G13210.1 58.000 150 51 2 34 177 22 165 8.16e-55 185
Msa0121330 AT4G13210.1 54.701 117 53 0 193 309 277 393 4.15e-36 135
Msa0121330 AT4G13210.2 58.000 150 51 2 34 177 59 202 8.96e-55 186
Msa0121330 AT4G13210.2 54.701 117 53 0 193 309 314 430 4.24e-36 136
Msa0121330 AT3G24230.1 53.704 162 66 2 34 189 70 228 8.81e-54 184
Msa0121330 AT3G24230.1 55.372 121 53 1 193 313 328 447 7.10e-40 147
Msa0121330 AT5G48900.1 47.805 205 91 4 3 198 5 202 1.54e-50 174
Msa0121330 AT5G48900.1 56.410 117 51 0 193 309 293 409 7.29e-35 132
Msa0121330 AT1G14420.1 45.699 186 83 3 24 191 46 231 4.75e-49 172
Msa0121330 AT1G14420.1 55.285 123 49 1 194 310 330 452 6.73e-38 142
Msa0121330 AT3G27400.2 50.282 177 74 3 21 191 26 194 7.17e-49 170
Msa0121330 AT3G27400.2 52.893 121 57 0 193 313 292 412 2.75e-39 144
Msa0121330 AT3G27400.1 50.282 177 74 3 21 191 22 190 7.70e-49 170
Msa0121330 AT3G27400.1 52.893 121 57 0 193 313 288 408 2.61e-39 144
Msa0121330 AT5G55720.1 41.000 200 96 5 10 202 1 185 4.07e-47 165
Msa0121330 AT5G55720.1 52.033 123 58 1 193 314 267 389 1.49e-33 129
Msa0121330 AT3G01270.1 54.135 133 55 1 62 188 116 248 2.13e-45 162
Msa0121330 AT3G01270.1 51.261 119 56 2 193 309 349 467 9.50e-32 125
Msa0121330 AT5G15110.1 51.449 138 61 1 67 198 118 255 4.96e-45 161
Msa0121330 AT5G15110.1 49.580 119 58 2 193 309 346 464 8.64e-31 122
Msa0121330 AT2G02720.1 43.548 186 87 3 21 188 39 224 9.31e-44 157
Msa0121330 AT2G02720.1 51.220 123 54 1 194 310 326 448 5.08e-34 131
Msa0121330 AT1G11920.1 52.000 150 63 3 63 206 29 175 1.27e-43 155
Msa0121330 AT1G11920.1 56.977 86 36 1 193 278 260 344 8.15e-28 113
Msa0121330 AT4G22080.1 52.985 134 56 2 71 198 47 179 1.42e-40 147
Msa0121330 AT4G22080.1 45.238 126 60 3 193 314 270 390 5.63e-25 105
Msa0121330 AT4G22090.1 54.331 127 51 2 71 191 47 172 2.15e-39 144
Msa0121330 AT4G22090.1 46.032 126 59 3 193 314 270 390 8.09e-25 104
Msa0121330 AT1G30350.1 51.200 125 54 2 71 189 36 159 1.38e-36 136
Msa0121330 AT1G30350.1 44.915 118 62 2 193 309 245 360 3.79e-24 102
Msa0121330 AT5G09280.1 42.157 102 49 3 104 196 6 106 1.79e-15 76.6
Msa0121330 AT3G55140.1 37.931 116 66 3 90 200 16 130 4.19e-11 63.9

Find 97 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGATTGTTGGCCCAATC+TGG 0.246902 1_3:-47130445 None:intergenic
ACTTCTAAGGCAACAGATTC+AGG 0.249046 1_3:-47129778 None:intergenic
AACATAAGAGTCCAACAAAA+AGG 0.254751 1_3:-47134361 None:intergenic
ACAATGGCTGCTGGTGTTCT+TGG 0.258365 1_3:+47133098 Msa0121330:CDS
TATCTTAAAGTACCAGGTTT+TGG 0.259866 1_3:-47130571 None:intergenic
CATTTCCAACAATCATCTAT+TGG 0.287315 1_3:-47130418 None:intergenic
TGATGTTCCATGGTTGAAAA+AGG 0.293620 1_3:-47129719 None:intergenic
AGGTTAGCAGATTGTGCTAT+AGG 0.311555 1_3:+47130469 Msa0121330:CDS
AATAGCTACAAAACCATTGA+TGG 0.315393 1_3:+47130673 Msa0121330:CDS
ATTTCCAACAATCATCTATT+GGG 0.325009 1_3:-47130417 None:intergenic
GATTATAGTATGTCATATTC+TGG 0.334113 1_3:+47134278 Msa0121330:CDS
TTAGGAAAATGCAGGTTGTT+TGG 0.336248 1_3:-47129681 None:intergenic
GCGAATGGACTTCGTGGAAT+TGG 0.339342 1_3:+47132952 Msa0121330:CDS
GCTGTTAAAGATCTATGGTT+AGG 0.343185 1_3:-47134391 None:intergenic
GCAGATTGTGCTATAGGGTT+TGG 0.343920 1_3:+47130475 Msa0121330:CDS
TGCTAACCTTTGACGATTGT+TGG 0.344322 1_3:-47130455 None:intergenic
ATGCTTAACCTCACTCTTCC+TGG 0.345866 1_3:+47129742 Msa0121330:CDS
TTAACATTGCCCTTGTCTTA+AGG 0.347810 1_3:-47134504 None:intergenic
GAAATCCTATCAGCAGCTTT+AGG 0.349951 1_3:-47133068 None:intergenic
TGTGTACCTGTGAACTTCTA+AGG 0.361301 1_3:-47129791 None:intergenic
GGTTAGCAGATTGTGCTATA+GGG 0.381880 1_3:+47130470 Msa0121330:CDS
GTGACGAAGAGGTTGGATTC+TGG 0.389452 1_3:+47132924 Msa0121330:CDS
TTAAGACAAGGGCAATGTTA+AGG 0.399441 1_3:+47134506 Msa0121330:three_prime_UTR
GGAATGGAAGGGTAAGTAGT+AGG 0.411886 1_3:-47129569 None:intergenic
GATGCAAGGTAACCGGTATA+CGG 0.413903 1_3:+47132875 Msa0121330:intron
AACTTAATCATCATGTTACT+TGG 0.416311 1_3:-47130631 None:intergenic
AGGAGCAAAGTAGTGAGATT+AGG 0.428527 1_3:-47129699 None:intergenic
GGTGCTGATGTTCATATTGT+TGG 0.431570 1_3:+47130700 Msa0121330:CDS
TTTGTTGATTCAGGTGATAA+GGG 0.433331 1_3:+47133011 Msa0121330:CDS
CTGTTAAAGATCTATGGTTA+GGG 0.438693 1_3:-47134390 None:intergenic
TTCAGAGAATGCCACGTTGT+AGG 0.439794 1_3:+47132553 Msa0121330:CDS
GACGATTGTTGGCCCAATCT+GGG 0.446148 1_3:-47130444 None:intergenic
TTGGAAATGCGACCCAGATT+GGG 0.450125 1_3:+47130432 Msa0121330:CDS
AAGGCAACAGATTCAGGGTC+TGG 0.459566 1_3:-47129772 None:intergenic
GTTTGGAACATTGTTGATGA+AGG 0.460039 1_3:-47129664 None:intergenic
GAAACCCAATAGATGATTGT+TGG 0.460719 1_3:+47130413 Msa0121330:CDS
TGTATGCTATTGGTGGAAGC+GGG 0.461262 1_3:+47132622 Msa0121330:CDS
GTTGGAAATGCGACCCAGAT+TGG 0.464792 1_3:+47130431 Msa0121330:CDS
TATCTCTGATGCAAGGTAAC+CGG 0.469363 1_3:+47132868 Msa0121330:intron
TCTAGTCTTACAATGGCTGC+TGG 0.469801 1_3:+47133089 Msa0121330:CDS
CTGGTGTTCTTGGTGTTGCT+AGG 0.475057 1_3:+47133108 Msa0121330:CDS
TTGATCCTAAAGCTGCTGAT+AGG 0.481095 1_3:+47133063 Msa0121330:CDS
CTATAATCACTACCACCGTT+TGG 0.488959 1_3:-47134263 None:intergenic
CAAAATGCCAAAGGAGGAAA+AGG 0.490161 1_3:+47130499 Msa0121330:CDS
ATGAGCAGGGACAATAATCT+AGG 0.491954 1_3:+47134224 Msa0121330:intron
TGGGAGATGTATGCTATTGG+TGG 0.495777 1_3:+47132615 Msa0121330:CDS
TTATACAAAATGAACCACTT+TGG 0.496510 1_3:+47130599 Msa0121330:CDS
CATCCTCAGGAGCCGTATAC+CGG 0.501557 1_3:-47132887 None:intergenic
TAGTGAGATTAGGAAAATGC+AGG 0.503825 1_3:-47129689 None:intergenic
TGCTTCGCGTCAACATCCTC+AGG 0.504471 1_3:-47132900 None:intergenic
GAAATCTGAAGGTGATGTTA+TGG 0.519098 1_3:+47132974 Msa0121330:CDS
ATCAGCACCACGACCATCAA+TGG 0.524009 1_3:-47130686 None:intergenic
AGAATGCCACGTTGTAGGCT+CGG 0.529636 1_3:+47132558 Msa0121330:CDS
CTCACTCTCCACTAGTGGAA+TGG 0.531516 1_3:-47129585 None:intergenic
TGGATTCTGGTGACAGCGAA+TGG 0.532614 1_3:+47132937 Msa0121330:CDS
GTTCATCATTGTCATCCCTC+AGG 0.533136 1_3:+47130778 Msa0121330:CDS
ATGTATGCTATTGGTGGAAG+CGG 0.534035 1_3:+47132621 Msa0121330:CDS
CTTCTAAGGCAACAGATTCA+GGG 0.534724 1_3:-47129777 None:intergenic
GATGTTCATATTGTTGGTGG+TGG 0.536484 1_3:+47130706 Msa0121330:CDS
CTGTTGCCTTAGAAGTTCAC+AGG 0.539920 1_3:+47129785 Msa0121330:CDS
CTCTCCACTAGTGGAATGGA+AGG 0.541289 1_3:-47129581 None:intergenic
GTGACAGCGAATGGACTTCG+TGG 0.543746 1_3:+47132946 Msa0121330:CDS
TTACCCTTCCATTCCACTAG+TGG 0.546463 1_3:+47129577 Msa0121330:five_prime_UTR
GCTGATGTTCATATTGTTGG+TGG 0.547478 1_3:+47130703 Msa0121330:CDS
AGATGGGAGATGTATGCTAT+TGG 0.550330 1_3:+47132612 Msa0121330:CDS
TTTGGAACATTGTTGATGAA+GGG 0.562353 1_3:-47129663 None:intergenic
GGTTAAGCATTGATGTTCCA+TGG 0.562902 1_3:-47129729 None:intergenic
GATTCAGGTGATAAGGGTGA+AGG 0.563107 1_3:+47133017 Msa0121330:CDS
TCAGGGTCTGGATGTTGACC+AGG 0.567446 1_3:-47129760 None:intergenic
TACAAAACCATTGATGGTCG+TGG 0.571754 1_3:+47130679 Msa0121330:CDS
GAAGCAAGTGACGAAGAGGT+TGG 0.573237 1_3:+47132917 Msa0121330:CDS
CACTTTGGAGAAAGCAGTCA+TGG 0.576400 1_3:+47129539 None:intergenic
ACTCACTCACTCTCCACTAG+TGG 0.593601 1_3:-47129590 None:intergenic
GAAGGTGATGTTATGGCGAA+TGG 0.601854 1_3:+47132981 Msa0121330:CDS
TCGTGGAATTGGAAATCTGA+AGG 0.604215 1_3:+47132963 Msa0121330:CDS
CTTGGAAACACAATCCAAAG+TGG 0.606839 1_3:-47130613 None:intergenic
ACGCGAAGCAAGTGACGAAG+AGG 0.613864 1_3:+47132913 Msa0121330:CDS
GTATGCTATTGGTGGAAGCG+GGG 0.614417 1_3:+47132623 Msa0121330:CDS
TAGGATTTCTAGTCTTACAA+TGG 0.616043 1_3:+47133082 Msa0121330:CDS
TATGCTATTGGTGGAAGCGG+GGG 0.619508 1_3:+47132624 Msa0121330:CDS
AATGCCAAAGGAGGAAAAGG+TGG 0.625369 1_3:+47130502 Msa0121330:CDS
GGGACAATAATCTAGGAATG+TGG 0.631048 1_3:+47134231 Msa0121330:intron
ATTGGGCCAACAATCGTCAA+AGG 0.631186 1_3:+47130449 Msa0121330:CDS
ATTGACAGCATCATTGTCTG+AGG 0.633209 1_3:-47130548 None:intergenic
GATGCTGTCAATCCAAAACC+TGG 0.634213 1_3:+47130559 Msa0121330:CDS
ATGTGGACAAGAGCACCAAA+CGG 0.637472 1_3:+47134248 Msa0121330:CDS
TAACCGGTATACGGCTCCTG+AGG 0.641263 1_3:+47132884 Msa0121330:CDS
GGGTTTGGACAAAATGCCAA+AGG 0.641402 1_3:+47130490 Msa0121330:CDS
ACTGACTGCTCCTTAAGACA+AGG 0.647323 1_3:+47134494 Msa0121330:three_prime_UTR
TTTGGACAAAATGCCAAAGG+AGG 0.659473 1_3:+47130493 Msa0121330:CDS
GATGTTGACCAGGAAGAGTG+AGG 0.666585 1_3:-47129750 None:intergenic
TCTCCACTAGTGGAATGGAA+GGG 0.696607 1_3:-47129580 None:intergenic
CTGACTGCTCCTTAAGACAA+GGG 0.706726 1_3:+47134495 Msa0121330:three_prime_UTR
TAAAATCATTGTTCACCACG+TGG 0.724046 1_3:-47132587 None:intergenic
TGGACAAGAGCACCAAACGG+TGG 0.743815 1_3:+47134251 Msa0121330:CDS
ATGTATCCGAGCCTACAACG+TGG 0.778122 1_3:-47132564 None:intergenic
TAGGCTCGGATACATCCACG+TGG 0.826381 1_3:+47132572 Msa0121330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATTTCTTTGTTTTT+TGG + chr1_3:47130043-47130062 Msa0121330:intron 10.0%
!!! AAAAAATTTCTTTGTTTTTT+GGG + chr1_3:47130044-47130063 Msa0121330:intron 10.0%
!!! TGAAAAAATTTCATTTTTTA+TGG + chr1_3:47130096-47130115 Msa0121330:intron 10.0%
!!! AAAAATACTACTATTTTTTT+AGG + chr1_3:47130146-47130165 Msa0121330:intron 10.0%
!!! AAAATACTACTATTTTTTTA+GGG + chr1_3:47130147-47130166 Msa0121330:intron 10.0%
!!! AACAATTTCATTTTATTTTT+TGG + chr1_3:47130176-47130195 Msa0121330:intron 10.0%
!!! ACAATTTCATTTTATTTTTT+GGG + chr1_3:47130177-47130196 Msa0121330:intron 10.0%
!!! CATATAAAAATTTCATTTTT+TGG + chr1_3:47130232-47130251 Msa0121330:intron 10.0%
!!! ATGTAACTTTTAAAAAATTA+CGG + chr1_3:47130930-47130949 Msa0121330:intron 10.0%
!!! CAATTAATTAACTTTTTTTT+AGG - chr1_3:47131154-47131173 None:intergenic 10.0%
!! AAAAAAAAGTTAATTAATTG+TGG + chr1_3:47131154-47131173 Msa0121330:intron 10.0%
!! AAAAAAAGTTAATTAATTGT+GGG + chr1_3:47131155-47131174 Msa0121330:intron 10.0%
!!! GATTTTTTTTTAACTTTATT+TGG + chr1_3:47131399-47131418 Msa0121330:intron 10.0%
!! AATTCCAAATAATAAATATT+TGG + chr1_3:47131756-47131775 Msa0121330:intron 10.0%
!!! TAAATGATGTATAATTTTAT+AGG - chr1_3:47131787-47131806 None:intergenic 10.0%
!!! TAATTTTTAAAATGTCAATT+AGG + chr1_3:47133363-47133382 Msa0121330:intron 10.0%
!! TAAAAAATAAAGGATAAAAA+TGG + chr1_3:47133663-47133682 Msa0121330:intron 10.0%
!!! TTTTATTTTGTTTTAGATTT+TGG + chr1_3:47133915-47133934 Msa0121330:intron 10.0%
!! CTTAATGATTATAAAGATAT+GGG - chr1_3:47129951-47129970 None:intergenic 15.0%
!! TCTTAATGATTATAAAGATA+TGG - chr1_3:47129952-47129971 None:intergenic 15.0%
!! TTAAAACAAGTTATGTAAAA+GGG + chr1_3:47129983-47130002 Msa0121330:intron 15.0%
!!! AAAAATTTCTTTGTTTTTTG+GGG + chr1_3:47130045-47130064 Msa0121330:intron 15.0%
!!! GTCATAACAAATTTTTTTTT+GGG + chr1_3:47130118-47130137 Msa0121330:intron 15.0%
!!! CAATTTCATTTTATTTTTTG+GGG + chr1_3:47130178-47130197 Msa0121330:intron 15.0%
!!! ATAAAAATTTCATTTTTTGG+AGG + chr1_3:47130235-47130254 Msa0121330:intron 15.0%
!!! TAAAAATTTCATTTTTTGGA+GGG + chr1_3:47130236-47130255 Msa0121330:intron 15.0%
!!! AAATAATTTTTTTTACCTGA+GGG - chr1_3:47130796-47130815 None:intergenic 15.0%
!!! TAAATAATTTTTTTTACCTG+AGG - chr1_3:47130797-47130816 None:intergenic 15.0%
!! AAAATTATTTAGTATACGAT+TGG + chr1_3:47130806-47130825 Msa0121330:intron 15.0%
!! TTATTATACATCAATTAAGT+AGG + chr1_3:47131019-47131038 Msa0121330:intron 15.0%
!! ATTAAAATCACATACTTATA+TGG - chr1_3:47131306-47131325 None:intergenic 15.0%
!!! TTTGTTGACTAAAATATAAA+AGG + chr1_3:47131543-47131562 Msa0121330:intron 15.0%
!!! TTGTTGACTAAAATATAAAA+GGG + chr1_3:47131544-47131563 Msa0121330:intron 15.0%
!!! AAATCTTACAAAACAAATTT+TGG - chr1_3:47133981-47134000 None:intergenic 15.0%
!!! TTTTGTAAGATTTTTTTTGA+AGG + chr1_3:47133988-47134007 Msa0121330:intron 15.0%
!!! AACTTGTTTTAAGTAATGTA+GGG - chr1_3:47129975-47129994 None:intergenic 20.0%
!!! TAACTTGTTTTAAGTAATGT+AGG - chr1_3:47129976-47129995 None:intergenic 20.0%
!! CTTAAAACAAGTTATGTAAA+AGG + chr1_3:47129982-47130001 Msa0121330:intron 20.0%
!!! GGTCATAACAAATTTTTTTT+TGG + chr1_3:47130117-47130136 Msa0121330:intron 20.0%
!! TGTATAAAAGTTCAAAATCA+TGG - chr1_3:47130865-47130884 None:intergenic 20.0%
!!! TAACTTTTAAAAAATTACGG+TGG + chr1_3:47130933-47130952 Msa0121330:intron 20.0%
!! AACATACTACAAATTAGAAT+AGG - chr1_3:47131241-47131260 None:intergenic 20.0%
!!! TATATGATTTCAAAAGTGTA+GGG - chr1_3:47131484-47131503 None:intergenic 20.0%
!!! TTATATGATTTCAAAAGTGT+AGG - chr1_3:47131485-47131504 None:intergenic 20.0%
!! GTGTCCAAATATTTATTATT+TGG - chr1_3:47131763-47131782 None:intergenic 20.0%
!! AAGTTAGTTAATAGTTAGTT+AGG - chr1_3:47132848-47132867 None:intergenic 20.0%
!! ATTCAATCAACTAGTAATAA+AGG - chr1_3:47133175-47133194 None:intergenic 20.0%
!! ACAAAAGTAACAAAAGTAAA+AGG - chr1_3:47133785-47133804 None:intergenic 20.0%
!! TTTGGTACAAATAATATCAA+AGG + chr1_3:47133933-47133952 Msa0121330:intron 20.0%
!!! ATACTTTTTTTTGACAAAAG+AGG + chr1_3:47134164-47134183 Msa0121330:intron 20.0%
! AAAAAGCTAAGAAAGAAACA+GGG - chr1_3:47129891-47129910 None:intergenic 25.0%
! ATTTCCAACAATCATCTATT+GGG - chr1_3:47130420-47130439 None:intergenic 25.0%
! TTATACAAAATGAACCACTT+TGG + chr1_3:47130599-47130618 Msa0121330:CDS 25.0%
! AACTTAATCATCATGTTACT+TGG - chr1_3:47130634-47130653 None:intergenic 25.0%
! AAAGCAACTAAATACATGTT+TGG - chr1_3:47130988-47131007 None:intergenic 25.0%
!! ATATGATTTCAAAAGTGTAG+GGG - chr1_3:47131483-47131502 None:intergenic 25.0%
! ACTAAAATATAAAAGGGTCT+TGG + chr1_3:47131550-47131569 Msa0121330:intron 25.0%
! AATTACACTTCCTCTAATTT+GGG - chr1_3:47131644-47131663 None:intergenic 25.0%
! TAATTACACTTCCTCTAATT+TGG - chr1_3:47131645-47131664 None:intergenic 25.0%
! GAATAATAAATGCTAAGAGA+AGG - chr1_3:47131914-47131933 None:intergenic 25.0%
! CACACAGAAAATATTAACTT+TGG - chr1_3:47132190-47132209 None:intergenic 25.0%
!! ATTTTCTGTGTGAACAAATA+AGG + chr1_3:47132198-47132217 Msa0121330:intron 25.0%
! ACAAAATTTGTAATGCTTGA+AGG + chr1_3:47132243-47132262 Msa0121330:intron 25.0%
! ATACCTTGACTAAAAATAGT+TGG - chr1_3:47132651-47132670 None:intergenic 25.0%
! TAAGAAATTAAGTGTGTGTA+TGG - chr1_3:47133319-47133338 None:intergenic 25.0%
!! AAATAAGTTTAGAATTGCGT+TGG - chr1_3:47133551-47133570 None:intergenic 25.0%
!!! TTTGTCGTTTTATAACTGTA+TGG + chr1_3:47133800-47133819 Msa0121330:intron 25.0%
!!! TTGTCGTTTTATAACTGTAT+GGG + chr1_3:47133801-47133820 Msa0121330:intron 25.0%
!! TTTTAAATACAAACCCACTT+GGG - chr1_3:47134108-47134127 None:intergenic 25.0%
! GATTATAGTATGTCATATTC+TGG + chr1_3:47134278-47134297 Msa0121330:CDS 25.0%
!! TTGAGCATCTTCAATTTTAT+TGG + chr1_3:47134424-47134443 Msa0121330:three_prime_UTR 25.0%
! TTTGGAACATTGTTGATGAA+GGG - chr1_3:47129666-47129685 None:intergenic 30.0%
GTAAGAAGAGAGAAAAATGA+GGG - chr1_3:47129859-47129878 None:intergenic 30.0%
AGTAAGAAGAGAGAAAAATG+AGG - chr1_3:47129860-47129879 None:intergenic 30.0%
AAAAGCTAAGAAAGAAACAG+GGG - chr1_3:47129890-47129909 None:intergenic 30.0%
CAAAAAGCTAAGAAAGAAAC+AGG - chr1_3:47129892-47129911 None:intergenic 30.0%
GATACCATAGAAAACTAACT+TGG + chr1_3:47129911-47129930 Msa0121330:intron 30.0%
!!! ATGTCCAAGTTAGTTTTCTA+TGG - chr1_3:47129918-47129937 None:intergenic 30.0%
TTATGTAAAAGGGTAGATCT+GGG + chr1_3:47129993-47130012 Msa0121330:intron 30.0%
AAATTCCTATTGTTCTTTGC+AGG + chr1_3:47130314-47130333 Msa0121330:intron 30.0%
! TTTTCAACATCATGTATGAC+AGG + chr1_3:47130391-47130410 Msa0121330:CDS 30.0%
CATTTCCAACAATCATCTAT+TGG - chr1_3:47130421-47130440 None:intergenic 30.0%
!! TATCTTAAAGTACCAGGTTT+TGG - chr1_3:47130574-47130593 None:intergenic 30.0%
AATAGCTACAAAACCATTGA+TGG + chr1_3:47130673-47130692 Msa0121330:CDS 30.0%
TCACATACTTATATGGTAGA+AGG - chr1_3:47131299-47131318 None:intergenic 30.0%
AAATATAAAAGGGTCTTGGA+TGG + chr1_3:47131554-47131573 Msa0121330:intron 30.0%
ACAAGACTAAACGAAACTTA+AGG + chr1_3:47131596-47131615 Msa0121330:intron 30.0%
CAAGACTAAACGAAACTTAA+GGG + chr1_3:47131597-47131616 Msa0121330:intron 30.0%
AAGACTAAACGAAACTTAAG+GGG + chr1_3:47131598-47131617 Msa0121330:intron 30.0%
CATTAGAGAAATACAGTACA+TGG + chr1_3:47131725-47131744 Msa0121330:intron 30.0%
ATTTACCACTTGAACTTAGA+AGG - chr1_3:47132067-47132086 None:intergenic 30.0%
! ACAATAGCATTCAACCATTT+TGG + chr1_3:47132519-47132538 Msa0121330:intron 30.0%
GAACTAATTGTTGACCAAAA+TGG - chr1_3:47132536-47132555 None:intergenic 30.0%
! TGAACAATGATTTTACGAGA+TGG + chr1_3:47132595-47132614 Msa0121330:CDS 30.0%
! GAACAATGATTTTACGAGAT+GGG + chr1_3:47132596-47132615 Msa0121330:CDS 30.0%
!! GAGCTAGATTTTGAAAATCA+AGG - chr1_3:47132757-47132776 None:intergenic 30.0%
GCAACAATGTTAGTTATCTT+TGG - chr1_3:47132804-47132823 None:intergenic 30.0%
ACATTGTTGCAAAACAAAAG+AGG + chr1_3:47132814-47132833 Msa0121330:intron 30.0%
!!! TTTTGTTGATTCAGGTGATA+AGG + chr1_3:47133010-47133029 Msa0121330:CDS 30.0%
!! TTTGTTGATTCAGGTGATAA+GGG + chr1_3:47133011-47133030 Msa0121330:CDS 30.0%
!! TAGGATTTCTAGTCTTACAA+TGG + chr1_3:47133082-47133101 Msa0121330:CDS 30.0%
GAAAAGAAGGAAAAGCAAAA+GGG - chr1_3:47133145-47133164 None:intergenic 30.0%
AGAAAAGAAGGAAAAGCAAA+AGG - chr1_3:47133146-47133165 None:intergenic 30.0%
TAAAGGATGCTTAGAAAAGA+AGG - chr1_3:47133158-47133177 None:intergenic 30.0%
CCAAATGAACACTAAAAACT+TGG - chr1_3:47133212-47133231 None:intergenic 30.0%
!!! CCAAGTTTTTAGTGTTCATT+TGG + chr1_3:47133209-47133228 Msa0121330:intron 30.0%
!!! TGTCGTTTTATAACTGTATG+GGG + chr1_3:47133802-47133821 Msa0121330:intron 30.0%
GTAAATTAGCCCATCTAAAT+TGG + chr1_3:47134010-47134029 Msa0121330:intron 30.0%
! CTTTTAAATACAAACCCACT+TGG - chr1_3:47134109-47134128 None:intergenic 30.0%
AAAAAGGGTTACAAGAGAAT+GGG - chr1_3:47134348-47134367 None:intergenic 30.0%
ACATAAGAGTCCAACAAAAA+GGG - chr1_3:47134363-47134382 None:intergenic 30.0%
AACATAAGAGTCCAACAAAA+AGG - chr1_3:47134364-47134383 None:intergenic 30.0%
CTGTTAAAGATCTATGGTTA+GGG - chr1_3:47134393-47134412 None:intergenic 30.0%
!!! GTTTTGCTGTTAAAGATCTA+TGG - chr1_3:47134399-47134418 None:intergenic 30.0%
! GTTTGGAACATTGTTGATGA+AGG - chr1_3:47129667-47129686 None:intergenic 35.0%
TTAGGAAAATGCAGGTTGTT+TGG - chr1_3:47129684-47129703 None:intergenic 35.0%
TAGTGAGATTAGGAAAATGC+AGG - chr1_3:47129692-47129711 None:intergenic 35.0%
TGATGTTCCATGGTTGAAAA+AGG - chr1_3:47129722-47129741 None:intergenic 35.0%
! TTGGAACAATGTAGGTTTTG+TGG - chr1_3:47129835-47129854 None:intergenic 35.0%
AAGAGAGAAAAATGAGGGTT+TGG - chr1_3:47129854-47129873 None:intergenic 35.0%
GTTATGTAAAAGGGTAGATC+TGG + chr1_3:47129992-47130011 Msa0121330:intron 35.0%
ACTTTCCTGCAAAGAACAAT+AGG - chr1_3:47130322-47130341 None:intergenic 35.0%
GAAACCCAATAGATGATTGT+TGG + chr1_3:47130413-47130432 Msa0121330:CDS 35.0%
! ACTGCATATCTTAAAGTACC+AGG - chr1_3:47130580-47130599 None:intergenic 35.0%
TAGTATACGATTGGTATCAC+TGG + chr1_3:47130815-47130834 Msa0121330:intron 35.0%
AGTATACGATTGGTATCACT+GGG + chr1_3:47130816-47130835 Msa0121330:intron 35.0%
TAAATGCTAAGAGAAGGTCT+TGG - chr1_3:47131908-47131927 None:intergenic 35.0%
TTATTCTACCATTGCACATG+TGG + chr1_3:47131928-47131947 Msa0121330:intron 35.0%
! TTTTGCCTTCTAAGTTCAAG+TGG + chr1_3:47132059-47132078 Msa0121330:intron 35.0%
! GTTCTAAATCCATAGTGTTC+TGG - chr1_3:47132291-47132310 None:intergenic 35.0%
! GGATTTAGAACAGAATCAGA+CGG + chr1_3:47132300-47132319 Msa0121330:intron 35.0%
GGAGATGGAATCTCAATATT+CGG + chr1_3:47132321-47132340 Msa0121330:intron 35.0%
GAGATGGAATCTCAATATTC+GGG + chr1_3:47132322-47132341 Msa0121330:intron 35.0%
TATTCGGGTCAAAAGACATA+TGG + chr1_3:47132337-47132356 Msa0121330:intron 35.0%
GGTTTAATAGACGTCGTTAT+GGG + chr1_3:47132393-47132412 Msa0121330:intron 35.0%
CTAGTAACATAACCTCGTTA+TGG - chr1_3:47132458-47132477 None:intergenic 35.0%
CATAACGAGGTTATGTTACT+AGG + chr1_3:47132456-47132475 Msa0121330:intron 35.0%
TAAAATCATTGTTCACCACG+TGG - chr1_3:47132590-47132609 None:intergenic 35.0%
!!! GGACCAACTATTTTTAGTCA+AGG + chr1_3:47132645-47132664 Msa0121330:CDS 35.0%
CTAACTTTATCTCTGATGCA+AGG + chr1_3:47132861-47132880 Msa0121330:intron 35.0%
GAAATCTGAAGGTGATGTTA+TGG + chr1_3:47132974-47132993 Msa0121330:CDS 35.0%
!!! GGAGCTTTTTTTGTTGATTC+AGG + chr1_3:47133002-47133021 Msa0121330:CDS 35.0%
AGTATCAGAAGCAATCAAGT+AGG - chr1_3:47133757-47133776 None:intergenic 35.0%
! TTTTCCGTGCCAATTTAGAT+GGG - chr1_3:47134022-47134041 None:intergenic 35.0%
! TTTTTCCGTGCCAATTTAGA+TGG - chr1_3:47134023-47134042 None:intergenic 35.0%
! AAATGTGTTGATGAATGAGC+AGG + chr1_3:47134210-47134229 Msa0121330:intron 35.0%
! AATGTGTTGATGAATGAGCA+GGG + chr1_3:47134211-47134230 Msa0121330:intron 35.0%
CAAAAAGGGTTACAAGAGAA+TGG - chr1_3:47134349-47134368 None:intergenic 35.0%
! TCTCTTGTAACCCTTTTTGT+TGG + chr1_3:47134350-47134369 Msa0121330:CDS 35.0%
GCTGTTAAAGATCTATGGTT+AGG - chr1_3:47134394-47134413 None:intergenic 35.0%
TTAACATTGCCCTTGTCTTA+AGG - chr1_3:47134507-47134526 None:intergenic 35.0%
TTAAGACAAGGGCAATGTTA+AGG + chr1_3:47134506-47134525 Msa0121330:three_prime_UTR 35.0%
AGGAGCAAAGTAGTGAGATT+AGG - chr1_3:47129702-47129721 None:intergenic 40.0%
!!! CTTTGCTCCTTTTTCAACCA+TGG + chr1_3:47129712-47129731 Msa0121330:CDS 40.0%
!! GGTTAAGCATTGATGTTCCA+TGG - chr1_3:47129732-47129751 None:intergenic 40.0%
!! CTTCTAAGGCAACAGATTCA+GGG - chr1_3:47129780-47129799 None:intergenic 40.0%
!! ACTTCTAAGGCAACAGATTC+AGG - chr1_3:47129781-47129800 None:intergenic 40.0%
TGTGTACCTGTGAACTTCTA+AGG - chr1_3:47129794-47129813 None:intergenic 40.0%
! ATGAGGGTTTGGAACAATGT+AGG - chr1_3:47129843-47129862 None:intergenic 40.0%
TGCTAACCTTTGACGATTGT+TGG - chr1_3:47130458-47130477 None:intergenic 40.0%
AGGTTAGCAGATTGTGCTAT+AGG + chr1_3:47130469-47130488 Msa0121330:CDS 40.0%
GGTTAGCAGATTGTGCTATA+GGG + chr1_3:47130470-47130489 Msa0121330:CDS 40.0%
TTTGGACAAAATGCCAAAGG+AGG + chr1_3:47130493-47130512 Msa0121330:CDS 40.0%
CAAAATGCCAAAGGAGGAAA+AGG + chr1_3:47130499-47130518 Msa0121330:CDS 40.0%
ATTGACAGCATCATTGTCTG+AGG - chr1_3:47130551-47130570 None:intergenic 40.0%
CTTGGAAACACAATCCAAAG+TGG - chr1_3:47130616-47130635 None:intergenic 40.0%
! TACAAAACCATTGATGGTCG+TGG + chr1_3:47130679-47130698 Msa0121330:CDS 40.0%
!! GGTGCTGATGTTCATATTGT+TGG + chr1_3:47130700-47130719 Msa0121330:CDS 40.0%
GCTGATGTTCATATTGTTGG+TGG + chr1_3:47130703-47130722 Msa0121330:CDS 40.0%
! GATGTTCATATTGTTGGTGG+TGG + chr1_3:47130706-47130725 Msa0121330:CDS 40.0%
TCATGTATGTGCCAGAATCA+CGG - chr1_3:47130963-47130982 None:intergenic 40.0%
TCTCTCAAATGTCATTGTCG+CGG + chr1_3:47131123-47131142 Msa0121330:intron 40.0%
ACTTCCTCTAATTTGGGTCA+TGG - chr1_3:47131638-47131657 None:intergenic 40.0%
CGGTTTAATAGACGTCGTTA+TGG + chr1_3:47132392-47132411 Msa0121330:intron 40.0%
ATGGTGTGAGAAATGGTTGT+TGG - chr1_3:47132439-47132458 None:intergenic 40.0%
AGATGGGAGATGTATGCTAT+TGG + chr1_3:47132612-47132631 Msa0121330:CDS 40.0%
ATGTATGCTATTGGTGGAAG+CGG + chr1_3:47132621-47132640 Msa0121330:CDS 40.0%
TATCTCTGATGCAAGGTAAC+CGG + chr1_3:47132868-47132887 Msa0121330:intron 40.0%
TCGTGGAATTGGAAATCTGA+AGG + chr1_3:47132963-47132982 Msa0121330:CDS 40.0%
TTGATCCTAAAGCTGCTGAT+AGG + chr1_3:47133063-47133082 Msa0121330:CDS 40.0%
GAAATCCTATCAGCAGCTTT+AGG - chr1_3:47133071-47133090 None:intergenic 40.0%
! GATTTTCGTCAACCCTAATG+TGG + chr1_3:47133291-47133310 Msa0121330:intron 40.0%
GTGTGTATGGAACCACATTA+GGG - chr1_3:47133306-47133325 None:intergenic 40.0%
TGTGTGTATGGAACCACATT+AGG - chr1_3:47133307-47133326 None:intergenic 40.0%
GAAAAGCTAGCAAGTCTTCT+AGG - chr1_3:47133861-47133880 None:intergenic 40.0%
TTAGCCCATCTAAATTGGCA+CGG + chr1_3:47134015-47134034 Msa0121330:intron 40.0%
TGAGAATGTGCTCTTCTTTG+AGG - chr1_3:47134058-47134077 None:intergenic 40.0%
! TCAAAGAAGAGCACATTCTC+AGG + chr1_3:47134057-47134076 Msa0121330:intron 40.0%
!! TTGGGTTAGTTCAGTGGTAT+TGG - chr1_3:47134090-47134109 None:intergenic 40.0%
ATGAGCAGGGACAATAATCT+AGG + chr1_3:47134224-47134243 Msa0121330:intron 40.0%
GGGACAATAATCTAGGAATG+TGG + chr1_3:47134231-47134250 Msa0121330:intron 40.0%
CTATAATCACTACCACCGTT+TGG - chr1_3:47134266-47134285 None:intergenic 40.0%
GGAATGGAAGGGTAAGTAGT+AGG - chr1_3:47129572-47129591 None:intergenic 45.0%
TTACCCTTCCATTCCACTAG+TGG + chr1_3:47129577-47129596 Msa0121330:five_prime_UTR 45.0%
TCTCCACTAGTGGAATGGAA+GGG - chr1_3:47129583-47129602 None:intergenic 45.0%
ATGCTTAACCTCACTCTTCC+TGG + chr1_3:47129742-47129761 Msa0121330:CDS 45.0%
CTGTTGCCTTAGAAGTTCAC+AGG + chr1_3:47129785-47129804 Msa0121330:CDS 45.0%
AAGAAAGAAACAGGGGAGAG+AGG - chr1_3:47129883-47129902 None:intergenic 45.0%
TTGGAAATGCGACCCAGATT+GGG + chr1_3:47130432-47130451 Msa0121330:CDS 45.0%
ATTGGGCCAACAATCGTCAA+AGG + chr1_3:47130449-47130468 Msa0121330:CDS 45.0%
GCAGATTGTGCTATAGGGTT+TGG + chr1_3:47130475-47130494 Msa0121330:CDS 45.0%
GGGTTTGGACAAAATGCCAA+AGG + chr1_3:47130490-47130509 Msa0121330:CDS 45.0%
AATGCCAAAGGAGGAAAAGG+TGG + chr1_3:47130502-47130521 Msa0121330:CDS 45.0%
! TTTGCCACCTTTTCCTCCTT+TGG - chr1_3:47130509-47130528 None:intergenic 45.0%
GATGCTGTCAATCCAAAACC+TGG + chr1_3:47130559-47130578 Msa0121330:CDS 45.0%
GTTCATCATTGTCATCCCTC+AGG + chr1_3:47130778-47130797 Msa0121330:CDS 45.0%
CTCTCAAATGTCATTGTCGC+GGG + chr1_3:47131124-47131143 Msa0121330:intron 45.0%
TCTCAAATGTCATTGTCGCG+GGG + chr1_3:47131125-47131144 Msa0121330:intron 45.0%
! TTGGGTCATGGCCTCTTTTA+GGG - chr1_3:47131626-47131645 None:intergenic 45.0%
! TTTGGGTCATGGCCTCTTTT+AGG - chr1_3:47131627-47131646 None:intergenic 45.0%
CAGATTGTCCACATGTGCAA+TGG - chr1_3:47131939-47131958 None:intergenic 45.0%
AGAACAGAATCAGACGGAGA+TGG + chr1_3:47132306-47132325 Msa0121330:intron 45.0%
ACACTCTCACGTTGCAAAGA+CGG + chr1_3:47132372-47132391 Msa0121330:intron 45.0%
CCTCGTTATGGTGTGAGAAA+TGG - chr1_3:47132446-47132465 None:intergenic 45.0%
CCATTTCTCACACCATAACG+AGG + chr1_3:47132443-47132462 Msa0121330:intron 45.0%
! TTCCTGAGTCTGGTTTGTAG+TGG - chr1_3:47132491-47132510 None:intergenic 45.0%
GTTACTTGCATTCCTGAGTC+TGG - chr1_3:47132501-47132520 None:intergenic 45.0%
TTCAGAGAATGCCACGTTGT+AGG + chr1_3:47132553-47132572 Msa0121330:CDS 45.0%
TGGGAGATGTATGCTATTGG+TGG + chr1_3:47132615-47132634 Msa0121330:CDS 45.0%
TGTATGCTATTGGTGGAAGC+GGG + chr1_3:47132622-47132641 Msa0121330:CDS 45.0%
GATGCAAGGTAACCGGTATA+CGG + chr1_3:47132875-47132894 Msa0121330:intron 45.0%
GAAGGTGATGTTATGGCGAA+TGG + chr1_3:47132981-47133000 Msa0121330:CDS 45.0%
GATTCAGGTGATAAGGGTGA+AGG + chr1_3:47133017-47133036 Msa0121330:CDS 45.0%
! GGGTGAAGGTAGTTTTCAGA+AGG + chr1_3:47133031-47133050 Msa0121330:CDS 45.0%
!! TCTAGTCTTACAATGGCTGC+TGG + chr1_3:47133089-47133108 Msa0121330:CDS 45.0%
!! GTGCTCTTCTTTGAGGTCTT+AGG - chr1_3:47134051-47134070 None:intergenic 45.0%
TACCACTGAACTAACCCAAG+TGG + chr1_3:47134091-47134110 Msa0121330:intron 45.0%
ACCACTGAACTAACCCAAGT+GGG + chr1_3:47134092-47134111 Msa0121330:intron 45.0%
!! ATGTGGACAAGAGCACCAAA+CGG + chr1_3:47134248-47134267 Msa0121330:CDS 45.0%
ACTGACTGCTCCTTAAGACA+AGG + chr1_3:47134494-47134513 Msa0121330:three_prime_UTR 45.0%
CTGACTGCTCCTTAAGACAA+GGG + chr1_3:47134495-47134514 Msa0121330:three_prime_UTR 45.0%
!! TTAAAATCAATAAAAAATAA+AGG + chr1_3:47133653-47133672 Msa0121330:intron 5.0%
CTCTCCACTAGTGGAATGGA+AGG - chr1_3:47129584-47129603 None:intergenic 50.0%
CTCACTCTCCACTAGTGGAA+TGG - chr1_3:47129588-47129607 None:intergenic 50.0%
ACTCACTCACTCTCCACTAG+TGG - chr1_3:47129593-47129612 None:intergenic 50.0%
GATGTTGACCAGGAAGAGTG+AGG - chr1_3:47129753-47129772 None:intergenic 50.0%
AAGGCAACAGATTCAGGGTC+TGG - chr1_3:47129775-47129794 None:intergenic 50.0%
GTTGGAAATGCGACCCAGAT+TGG + chr1_3:47130431-47130450 Msa0121330:CDS 50.0%
GACGATTGTTGGCCCAATCT+GGG - chr1_3:47130447-47130466 None:intergenic 50.0%
TGACGATTGTTGGCCCAATC+TGG - chr1_3:47130448-47130467 None:intergenic 50.0%
ATCAGCACCACGACCATCAA+TGG - chr1_3:47130689-47130708 None:intergenic 50.0%
! TGGGTCATGGCCTCTTTTAG+GGG - chr1_3:47131625-47131644 None:intergenic 50.0%
GAGGCCATGACCCAAATTAG+AGG + chr1_3:47131631-47131650 Msa0121330:intron 50.0%
CGTTCGAGTCCAGAACACTA+TGG + chr1_3:47132279-47132298 Msa0121330:intron 50.0%
! TGCAACGTGAGAGTGTGCAA+TGG - chr1_3:47132368-47132387 None:intergenic 50.0%
! CTGGTTTGTAGTGGTCACTG+TGG - chr1_3:47132482-47132501 None:intergenic 50.0%
GACCACTACAAACCAGACTC+AGG + chr1_3:47132486-47132505 Msa0121330:intron 50.0%
AGAATGCCACGTTGTAGGCT+CGG + chr1_3:47132558-47132577 Msa0121330:CDS 50.0%
ATGTATCCGAGCCTACAACG+TGG - chr1_3:47132567-47132586 None:intergenic 50.0%
GTATGCTATTGGTGGAAGCG+GGG + chr1_3:47132623-47132642 Msa0121330:CDS 50.0%
TATGCTATTGGTGGAAGCGG+GGG + chr1_3:47132624-47132643 Msa0121330:CDS 50.0%
GAAGCAAGTGACGAAGAGGT+TGG + chr1_3:47132917-47132936 Msa0121330:CDS 50.0%
GTGACGAAGAGGTTGGATTC+TGG + chr1_3:47132924-47132943 Msa0121330:CDS 50.0%
TGGATTCTGGTGACAGCGAA+TGG + chr1_3:47132937-47132956 Msa0121330:CDS 50.0%
! GCGAATGGACTTCGTGGAAT+TGG + chr1_3:47132952-47132971 Msa0121330:CDS 50.0%
ACAATGGCTGCTGGTGTTCT+TGG + chr1_3:47133098-47133117 Msa0121330:CDS 50.0%
!! CTGGTGTTCTTGGTGTTGCT+AGG + chr1_3:47133108-47133127 Msa0121330:CDS 50.0%
!! ACCCACTTGGGTTAGTTCAG+TGG - chr1_3:47134096-47134115 None:intergenic 50.0%
TCAGGGTCTGGATGTTGACC+AGG - chr1_3:47129763-47129782 None:intergenic 55.0%
CTTAAGGGGCCCCCTAAAAG+AGG + chr1_3:47131612-47131631 Msa0121330:intron 55.0%
! GGGTCATGGCCTCTTTTAGG+GGG - chr1_3:47131624-47131643 None:intergenic 55.0%
TAGGCTCGGATACATCCACG+TGG + chr1_3:47132572-47132591 Msa0121330:CDS 55.0%
TAACCGGTATACGGCTCCTG+AGG + chr1_3:47132884-47132903 Msa0121330:CDS 55.0%
CATCCTCAGGAGCCGTATAC+CGG - chr1_3:47132890-47132909 None:intergenic 55.0%
TGCTTCGCGTCAACATCCTC+AGG - chr1_3:47132903-47132922 None:intergenic 55.0%
ACGCGAAGCAAGTGACGAAG+AGG + chr1_3:47132913-47132932 Msa0121330:CDS 55.0%
! GTGACAGCGAATGGACTTCG+TGG + chr1_3:47132946-47132965 Msa0121330:CDS 55.0%
!! TGGACAAGAGCACCAAACGG+TGG + chr1_3:47134251-47134270 Msa0121330:CDS 55.0%
ACGGTGGATCGCCGTGATTC+TGG + chr1_3:47130949-47130968 Msa0121330:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 47129545 47134542 47129545 ID=Msa0121330;Name=Msa0121330
chr1_3 mRNA 47129545 47134542 47129545 ID=Msa0121330-mRNA-1;Parent=Msa0121330;Name=Msa0121330-mRNA-1;_AED=0.16;_eAED=0.16;_QI=131|0.6|0.5|1|0.6|0.5|6|133|371
chr1_3 exon 47129545 47129806 47129545 ID=Msa0121330-mRNA-1:exon:14171;Parent=Msa0121330-mRNA-1
chr1_3 exon 47130336 47130580 47130336 ID=Msa0121330-mRNA-1:exon:14172;Parent=Msa0121330-mRNA-1
chr1_3 exon 47130599 47130799 47130599 ID=Msa0121330-mRNA-1:exon:14173;Parent=Msa0121330-mRNA-1
chr1_3 exon 47132553 47132666 47132553 ID=Msa0121330-mRNA-1:exon:14174;Parent=Msa0121330-mRNA-1
chr1_3 exon 47132883 47133129 47132883 ID=Msa0121330-mRNA-1:exon:14175;Parent=Msa0121330-mRNA-1
chr1_3 exon 47134232 47134542 47134232 ID=Msa0121330-mRNA-1:exon:14176;Parent=Msa0121330-mRNA-1
chr1_3 five_prime_UTR 47129545 47129675 47129545 ID=Msa0121330-mRNA-1:five_prime_utr;Parent=Msa0121330-mRNA-1
chr1_3 CDS 47129676 47129806 47129676 ID=Msa0121330-mRNA-1:cds;Parent=Msa0121330-mRNA-1
chr1_3 CDS 47130336 47130580 47130336 ID=Msa0121330-mRNA-1:cds;Parent=Msa0121330-mRNA-1
chr1_3 CDS 47130599 47130799 47130599 ID=Msa0121330-mRNA-1:cds;Parent=Msa0121330-mRNA-1
chr1_3 CDS 47132553 47132666 47132553 ID=Msa0121330-mRNA-1:cds;Parent=Msa0121330-mRNA-1
chr1_3 CDS 47132883 47133129 47132883 ID=Msa0121330-mRNA-1:cds;Parent=Msa0121330-mRNA-1
chr1_3 CDS 47134232 47134409 47134232 ID=Msa0121330-mRNA-1:cds;Parent=Msa0121330-mRNA-1
chr1_3 three_prime_UTR 47134410 47134542 47134410 ID=Msa0121330-mRNA-1:three_prime_utr;Parent=Msa0121330-mRNA-1
Gene Sequence

>Msa0121330

ATGTTCCAAACAACCTGCATTTTCCTAATCTCACTACTTTGCTCCTTTTTCAACCATGGAACATCAATGCTTAACCTCACTCTTCCTGGTCAACATCCAGACCCTGAATCTGTTGCCTTAGAAGTTCACAGGAAAGTGAATGTTTCAATGTTAAGAAGAGAAATGCTATCAATATCAAACAAAGAGTTTTCAACATCATGTATGACAGGAAACCCAATAGATGATTGTTGGAAATGCGACCCAGATTGGGCCAACAATCGTCAAAGGTTAGCAGATTGTGCTATAGGGTTTGGACAAAATGCCAAAGGAGGAAAAGGTGGCAAATTTTACATTGTCACAGATTCCTCAGACAATGATGCTGTCAATCCAAAACCTGTTATACAAAATGAACCACTTTGGATTGTGTTTCCAAGTAACATGATGATTAAGTTGTCTCAAGAGCTTATTTTTAATAGCTACAAAACCATTGATGGTCGTGGTGCTGATGTTCATATTGTTGGTGGTGGTTGCATCACTCTTCAGTATATAAGTAATGTTATTATACATAACATTCATGTTCATCATTGTCATCCCTCAGTTCAGAGAATGCCACGTTGTAGGCTCGGATACATCCACGTGGTGAACAATGATTTTACGAGATGGGAGATGTATGCTATTGGTGGAAGCGGGGGACCAACTATTTTTAGTCAAGGTAACCGGTATACGGCTCCTGAGGATGTTGACGCGAAGCAAGTGACGAAGAGGTTGGATTCTGGTGACAGCGAATGGACTTCGTGGAATTGGAAATCTGAAGGTGATGTTATGGCGAATGGAGCTTTTTTTGTTGATTCAGGTGATAAGGGTGAAGGTAGTTTTCAGAAGGCTTATAGTGTTGATCCTAAAGCTGCTGATAGGATTTCTAGTCTTACAATGGCTGCTGGTGTTCTTGGTGTTGCTAGGGACAATAATCTAGGAATGTGGACAAGAGCACCAAACGGTGGTAGTGATTATAGTATGTCATATTCTGGAGAAGAGTACACAGATGACATGTCAAAGAGTACAATGTTGTTGACCCATTCTCTTGTAACCCTTTTTGTTGGACTCTTATGTTTGTTGTCCCTAACCATAGATCTTTAA
Protein sequence

>Msa0121330

MFQTTCIFLISLLCSFFNHGTSMLNLTLPGQHPDPESVALEVHRKVNVSMLRREMLSISNKEFSTSCMTGNPIDDCWKCDPDWANNRQRLADCAIGFGQNAKGGKGGKFYIVTDSSDNDAVNPKPVIQNEPLWIVFPSNMMIKLSQELIFNSYKTIDGRGADVHIVGGGCITLQYISNVIIHNIHVHHCHPSVQRMPRCRLGYIHVVNNDFTRWEMYAIGGSGGPTIFSQGNRYTAPEDVDAKQVTKRLDSGDSEWTSWNWKSEGDVMANGAFFVDSGDKGEGSFQKAYSVDPKAADRISSLTMAAGVLGVARDNNLGMWTRAPNGGSDYSMSYSGEEYTDDMSKSTMLLTHSLVTLFVGLLCLLSLTIDL*