AlfalfaGEDB | Msa0966320

M. sativa cultivar zhongmu-4 / Msa0966320

KEGG: -
GO: -
PFAM: PF02985.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0966320	sp\|F4IK92\|TORL2_ARATH	41.748	824	439	17	11	821	25	820	0.0	563

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0966320	A0A072UCW5	94.411	823	39	3	1	823	1	816	0.0	1595

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003080	Msa0966320	0.824434	8.088793e-54	-8.615850e-47
Msa0005020	Msa0966320	0.837612	4.691188e-57	-8.615850e-47
Msa0005890	Msa0966320	0.812528	4.051187e-51	-8.615850e-47
Msa0016040	Msa0966320	0.859169	4.980989e-63	-8.615850e-47
Msa0019900	Msa0966320	0.816077	6.658074e-52	-8.615850e-47
Msa0019930	Msa0966320	0.832342	9.983336e-56	-8.615850e-47
Msa0020540	Msa0966320	0.821592	3.719415e-53	-8.615850e-47
Msa0025160	Msa0966320	0.810388	1.181601e-50	-8.615850e-47
Msa0025580	Msa0966320	0.847264	1.291224e-59	-8.615850e-47
Msa0027280	Msa0966320	0.819391	1.190581e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0966320	MtrunA17_Chr6g0488831	94.411	823	39	3	1	823	1	816	0.0	1595
Msa0966320	MtrunA17_Chr3g0098141	25.000	880	546	20	12	792	27	891	1.93e-65	235
Msa0966320	MtrunA17_Chr4g0059611	36.268	284	176	1	7	290	1	279	1.06e-49	185
Msa0966320	MtrunA17_Chr5g0415131	26.950	564	348	13	13	556	22	541	2.35e-44	171

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0966320	AT2G07170.1	41.748	824	439	17	11	821	25	820	0.0	563
Msa0966320	AT1G27210.1	30.669	538	325	12	12	548	34	524	4.35e-53	196
Msa0966320	AT4G27060.1	36.949	295	177	4	12	299	41	333	7.65e-49	186
Msa0966320	AT4G27060.1	21.680	369	227	13	494	817	504	855	3.16e-17	87.0
Msa0966320	AT1G50890.1	33.564	289	181	5	12	291	36	322	8.45e-45	174
Msa0966320	AT1G50890.1	24.296	284	193	8	501	777	503	771	6.79e-15	79.7
Msa0966320	AT1G59850.1	33.333	282	181	2	12	291	21	297	7.47e-37	145
Msa0966320	AT5G62580.2	32.056	287	181	5	14	290	7	289	8.70e-34	138
Msa0966320	AT5G62580.1	32.056	287	181	5	14	290	7	289	8.70e-34	138

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Find 163 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGATGCATCAGTTAC+AGG	0.369760	6_3:+94514519	Msa0966320:CDS
AAAGCATGTCAAAATGATGT+TGG	0.506304	6_3:+94514028	Msa0966320:CDS
AAAGCGGCTTCTGTTGCGTT+GGG	0.473923	6_3:+94513309	Msa0966320:CDS
AAATATTGGAAAATGCTTCC+GGG	0.434596	6_3:+94513558	Msa0966320:CDS
AAATTATCCTCTCTGACTTT+TGG	0.155108	6_3:-94515264	None:intergenic
AACAACCGCAGCAACAATTT+TGG	0.226264	6_3:-94512875	None:intergenic
AACATCTCTATGTCTGGACT+CGG	0.546866	6_3:-94513948	None:intergenic
AACGAGAATAAGTTCGTCAC+CGG	0.604731	6_3:-94515481	None:intergenic
AACGGTTTGCTTCTCACATT+TGG	0.314748	6_3:-94514319	None:intergenic
AACGTGCCAATCATCTCTTT+TGG	0.320011	6_3:-94514065	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CACAATGCATTAAACAAAGT+TGG	+	chr6_3:94512655-94512674	Msa0966320:CDS	30.0%
GAATCAAGGTAATGAAGATA+AGG	+	chr6_3:94512975-94512994	Msa0966320:CDS	30.0%
GTTGAATAGGTGTATTATTC+AGG	+	chr6_3:94513200-94513219	Msa0966320:CDS	30.0%
TTCGTTCTTGTCATCTTTAA+GGG	+	chr6_3:94513353-94513372	Msa0966320:CDS	30.0%
AAATATTGGAAAATGCTTCC+GGG	+	chr6_3:94513558-94513577	Msa0966320:CDS	30.0%
CTTTCTACAGAAAATATCTG+CGG	+	chr6_3:94513887-94513906	Msa0966320:intron	30.0%
AAAGCATGTCAAAATGATGT+TGG	+	chr6_3:94514028-94514047	Msa0966320:CDS	30.0%
TACTCTCAAACTCTTCATTA+TGG	-	chr6_3:94514129-94514148	None:intergenic	30.0%
ATTGTTTACAAAGCAGTCAT+GGG	-	chr6_3:94514287-94514306	None:intergenic	30.0%
CAACTTATTACTTCCTCTAA+CGG	-	chr6_3:94514340-94514359	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	94512468	94516392	94512468	ID=Msa0966320;Name=Msa0966320
chr6_3	mRNA	94512468	94516392	94512468	ID=Msa0966320-mRNA-1;Parent=Msa0966320;Name=Msa0966320-mRNA-1;_AED=0.06;_eAED=0.06;_QI=136\|0.6\|0.66\|0.83\|0.6\|0.83\|6\|170\|823
chr6_3	exon	94512468	94513416	94512468	ID=Msa0966320-mRNA-1:exon:17441;Parent=Msa0966320-mRNA-1
chr6_3	exon	94513522	94513624	94513522	ID=Msa0966320-mRNA-1:exon:17442;Parent=Msa0966320-mRNA-1
chr6_3	exon	94513897	94514540	94513897	ID=Msa0966320-mRNA-1:exon:17443;Parent=Msa0966320-mRNA-1
chr6_3	exon	94514925	94515639	94514925	ID=Msa0966320-mRNA-1:exon:17444;Parent=Msa0966320-mRNA-1
chr6_3	exon	94515959	94515969	94515959	ID=Msa0966320-mRNA-1:exon:17445;Parent=Msa0966320-mRNA-1
chr6_3	exon	94516037	94516392	94516037	ID=Msa0966320-mRNA-1:exon:17446;Parent=Msa0966320-mRNA-1
chr6_3	five_prime_UTR	94512468	94512603	94512468	ID=Msa0966320-mRNA-1:five_prime_utr;Parent=Msa0966320-mRNA-1
chr6_3	CDS	94512604	94513416	94512604	ID=Msa0966320-mRNA-1:cds;Parent=Msa0966320-mRNA-1
chr6_3	CDS	94513522	94513624	94513522	ID=Msa0966320-mRNA-1:cds;Parent=Msa0966320-mRNA-1
chr6_3	CDS	94513897	94514540	94513897	ID=Msa0966320-mRNA-1:cds;Parent=Msa0966320-mRNA-1
chr6_3	CDS	94514925	94515639	94514925	ID=Msa0966320-mRNA-1:cds;Parent=Msa0966320-mRNA-1
chr6_3	CDS	94515959	94515969	94515959	ID=Msa0966320-mRNA-1:cds;Parent=Msa0966320-mRNA-1
chr6_3	CDS	94516037	94516222	94516037	ID=Msa0966320-mRNA-1:cds;Parent=Msa0966320-mRNA-1
chr6_3	three_prime_UTR	94516223	94516392	94516223	ID=Msa0966320-mRNA-1:three_prime_utr;Parent=Msa0966320-mRNA-1

Gene Sequence

>Msa0966320

ATGAAGACCAACAACAAGCTGAGTAGTAATTTCGAGATGAAGCAAAGAGTACACAATGCATTAAACAAAGTTGGAGATCGAGACACACACGATATCGGAACCAAAGAACTCGAAACAATTCTCCACTCATTAACTCCACATTCCATCTCCTCATTCCTCTCTTGCATTCTCGACATTCACTCCGAACTAAAACCTTCCATTAGAAAACAATCTCTCAAACTCATCGCGACATTAGCCACTGTTTATGAATCTCTTATACTCCCACATCTCACCAAAATTGTTGCTGCGGTTGTTAAATGTTTTAGGGATCCAGATTCTTCTGTTAGAGAAGGTTGTATTGAAATGTTCGCGGTTTTAGCTTTGAAATTAGCGAATCAAGGTAATGAAGATAAGGTGTTTGTGTTATTGTCTAGTCCTATTTTCGATGCTCTTGTTGGTGAACAGAATAAGCATGTTCAATCTGCTTCAGCGTTTTGTCTTGCTGGTATTGTACAAAACACGCGGAATCCTCCTGTTTCGGTTTTGTATGGTATGTTGATTAGGATTCTTAAGTTGCTTAAGAATCCACATTTTATGGCGAAACCTGCGTTGATTGAGTTGAATAGGTGTATTATTCAGGCTGGTGGAGCTTCGACGCAGAGTGTTCTTTTGACGGCTGTTGGTAGTATTAGAGAGGGTTTGAAAGATACGGATTGGAAAACACGTAAAGCGGCTTCTGTTGCGTTGGGACAAATTGCTTTGGCTCGTGCTTCGTTCTTGTCATCTTTAAGGGTTTCTTGTATTCATTCTCTTGATTCCTCGAGGTTTGACAAGGTGAAACCAGTGAGGGATGCGGTTTTACAAGCTCTTAAATATTGGAAAATGCTTCCGGGTCATTATACTACCGAGCCTTCAGAAACTGGATCCTCCTTCAAAGAAAATATCTGCGGAGGTGACTCTGTGGATCTCAGTAGTACTACTACTACCACCGAGTCCAGACATAGAGATGTTAGGCTCCAAAAACTTAATATGAAATCAACTACGGGAAGGATTCCTTTGTCTGTAAGAAAAGCATGTCAAAATGATGTTGGACATCTTCGTCATCCCAAAAGAGATGATTGGCACGTTGAAGTTGCAGTCCCCAGAACACATTCTGTAGTGGAATTCCATAATGAAGAGTTTGAGAGTAGTTCTGTCACCAAGCCGCTACAAACAATGAGTCTTGATGTTACAAGCATGCAGGATGTGGGTTATGAATATGGGCCTATGGATGACAAGCAGGAGTGTTCTTCTGTTTCTAACTATGAAACGAAGTTTTTAACTTCCCATGACTGCTTTGTAAACAATGGACTACCGAAGCCAAATGTGAGAAGCAAACCGTTAGAGGAAGTAATAAGTTGTAATGAACAAATGTACTCCATAAAGATGCAGGATCCTAAAAGTTCTGATTCGACAGTCACCGAGTCAAGTCCCCAAATTACACATCAATGTTGTGTACAAAGGGCAAATGAAATGACTTGTATTCGGAATCAACTTTCAGATATTGAAATCAAACAGGAAAACATGATGCATCAGTTACAGATTTTTACAACTGGCATAATGGACGCCCTTTCTACAATCCAATCAAGAATGGTAGGCTTGGAGAATGTAATTGATAGATTATCTCAAGAATCTTTAAAAGGAGGAAGACATTCCTTTTCAGAAAACTCCAAATTTGTGAGGCAGAGCCAAAATGTCGCTTCTCCCAGGATCTCCGTATGCACTCCAAAGCCATCTTCAGAAACTAGTACTAAGCAATCAGGCTTGTTTTCGGTGAAAAGTAGTGAAAGTTTGGAAAAAAGATCATTTTCAAGGAGCCAACCGAGGATTCATGCTGGAGATAGTGTGGATATGTGTAAAAGTTACAAGGTCAAGACTGCCCCAAAAGTCAGAGAGGATAATTTGAACTGCTTAGGGGAGGATAGACAAAGCACGAGTTCTGCTCAAGTGAGAAAGAATGGTAGTATCTTTTCCTCTGCTGCCAGAACCAATGCTAGAAATGGTTGTCCTCCCGAGAGTAACACAAACTACTGGAAATGTGTGAATCGTCTTGTATGTGAAGGAGATCTAAACTCTGCATATATGGAAGCTTTGTGTTCCGGTGACGAACTTATTCTCGTTGAGCTTCTGAATAAAACCGGTCCAGTTATAGAAAGTTTATCCATGAAAACTGTCAATGTTCTTCTTAGCACTTTAGCACCGTATCTTCTGGAAGGAAAAATTTTCAATACCATAATCCCATGGCTGCAGCAGGCAAGATATTTTGTAGTTGTTGAAATGAGCACCGTCCACGGACCAAATTGCATTGCTCTTTCAATTGAAGTCGAAGAGCAGCTTTTATATGCAATTCAAGAGGTTGTGAACTTGAATATTTTCAGCCATGCAGAAAAGAGGCGTGCAGCTGAATTAGCAATAAAATTACATCATATCTGGAGTAAGATTAGTGAAAGTTAA

Protein sequence

>Msa0966320

MKTNNKLSSNFEMKQRVHNALNKVGDRDTHDIGTKELETILHSLTPHSISSFLSCILDIHSELKPSIRKQSLKLIATLATVYESLILPHLTKIVAAVVKCFRDPDSSVREGCIEMFAVLALKLANQGNEDKVFVLLSSPIFDALVGEQNKHVQSASAFCLAGIVQNTRNPPVSVLYGMLIRILKLLKNPHFMAKPALIELNRCIIQAGGASTQSVLLTAVGSIREGLKDTDWKTRKAASVALGQIALARASFLSSLRVSCIHSLDSSRFDKVKPVRDAVLQALKYWKMLPGHYTTEPSETGSSFKENICGGDSVDLSSTTTTTESRHRDVRLQKLNMKSTTGRIPLSVRKACQNDVGHLRHPKRDDWHVEVAVPRTHSVVEFHNEEFESSSVTKPLQTMSLDVTSMQDVGYEYGPMDDKQECSSVSNYETKFLTSHDCFVNNGLPKPNVRSKPLEEVISCNEQMYSIKMQDPKSSDSTVTESSPQITHQCCVQRANEMTCIRNQLSDIEIKQENMMHQLQIFTTGIMDALSTIQSRMVGLENVIDRLSQESLKGGRHSFSENSKFVRQSQNVASPRISVCTPKPSSETSTKQSGLFSVKSSESLEKRSFSRSQPRIHAGDSVDMCKSYKVKTAPKVREDNLNCLGEDRQSTSSAQVRKNGSIFSSAARTNARNGCPPESNTNYWKCVNRLVCEGDLNSAYMEALCSGDELILVELLNKTGPVIESLSMKTVNVLLSTLAPYLLEGKIFNTIIPWLQQARYFVVVEMSTVHGPNCIALSIEVEEQLLYAIQEVVNLNIFSHAEKRRAAELAIKLHHIWSKISES*