AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1370210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAATAGGTTTACACTTT+TGG 0.189771 tig0019103:+1547 Msa1370210:five_prime_UTR
TGTAAACCTATTAAAGAAAA+TGG 0.246709 tig0019103:-1540 Msa1370210:intergenic
TCTACGGTGTGTGAACTTAC+AGG 0.303289 tig0019103:-2770 Msa1370210:intergenic
TCCACGGTGTGTGAACTTAC+AGG 0.384784 tig0019103:-2797 Msa1370210:intergenic
GGGTTGAAACTGCTCAGTTC+AGG 0.385105 tig0019103:+1594 Msa1370210:CDS
AGCAGTTTCAACCCCGGCCA+TGG 0.495635 tig0019103:-1585 Msa1370210:intergenic
ACTGCAATTTGTGAATGATT+CGG 0.531479 tig0019103:+2736 Msa1370210:CDS
TAACATGATATGAATGTCCA+CGG 0.623472 tig0019103:-2813 Msa1370210:intergenic
TCCTGTAAGTTCACACACCG+TGG 0.645220 tig0019103:+2796 Msa1370210:CDS
TGAACTTACAGGATTGTCTA+CGG 0.659877 tig0019103:-2786 Msa1370200:intergenic
GAACTGAGCAGTTTCAACCC+CGG 0.704213 tig0019103:-1591 Msa1370200:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCCAATAAAAATTTTAATAT+CGG - tig0019103:1666-1685 Msa1370210:intergenic 10.0%
!! AATTATATATGAAAACATTA+TGG + tig0019103:1717-1736 Msa1370210:intron 10.0%
!!! TCCAATAAAAATTTTAATAT+CGG - tig0019103:1666-1685 Msa1370210:intergenic 10.0%
!! AATTATATATGAAAACATTA+TGG + tig0019103:1717-1736 Msa1370210:intron 10.0%
!!! AATTTTAATATCGGTTAAAA+TGG - tig0019103:1657-1676 Msa1370210:intergenic 15.0%
!!! ACCGATATTAAAATTTTTAT+TGG + tig0019103:1662-1681 Msa1370210:intron 15.0%
!! ATATAATTCATAAATTGCTT+TGG - tig0019103:2323-2342 Msa1370210:intergenic 15.0%
!! AAGCAATTTATGAATTATAT+TGG + tig0019103:2323-2342 Msa1370210:intron 15.0%
!! AGTATACTTCAAATAAAATA+AGG - tig0019103:2684-2703 Msa1370210:intergenic 15.0%
!!! AATTTTAATATCGGTTAAAA+TGG - tig0019103:1657-1676 Msa1370210:intergenic 15.0%
!!! ACCGATATTAAAATTTTTAT+TGG + tig0019103:1662-1681 Msa1370210:intron 15.0%
!! ATATAATTCATAAATTGCTT+TGG - tig0019103:2323-2342 Msa1370210:intergenic 15.0%
!! AAGCAATTTATGAATTATAT+TGG + tig0019103:2323-2342 Msa1370210:intron 15.0%
!! AGTATACTTCAAATAAAATA+AGG - tig0019103:2684-2703 Msa1370210:intergenic 15.0%
!!! ATTGATCCATTTTCTTTAAT+AGG + tig0019103:1534-1553 Msa1370210:five_prime_UTR 20.0%
!! TGTAAACCTATTAAAGAAAA+TGG - tig0019103:1543-1562 Msa1370210:intergenic 20.0%
!! TAGTATAACAATCTGTTATT+TGG + tig0019103:1740-1759 Msa1370210:intron 20.0%
!! TGTAATTCTCTATAGAAAAA+TGG + tig0019103:2367-2386 Msa1370210:intron 20.0%
!! TTCTCATTGTATACAATATT+AGG - tig0019103:2481-2500 Msa1370210:intergenic 20.0%
!!! ATTGATCCATTTTCTTTAAT+AGG + tig0019103:1534-1553 Msa1370210:five_prime_UTR 20.0%
!! TGTAAACCTATTAAAGAAAA+TGG - tig0019103:1543-1562 Msa1370210:intergenic 20.0%
!! TAGTATAACAATCTGTTATT+TGG + tig0019103:1740-1759 Msa1370210:intron 20.0%
!! TGTAATTCTCTATAGAAAAA+TGG + tig0019103:2367-2386 Msa1370210:intron 20.0%
!! TTCTCATTGTATACAATATT+AGG - tig0019103:2481-2500 Msa1370210:intergenic 20.0%
!! CTTTAATAGGTTTACACTTT+TGG + tig0019103:1547-1566 Msa1370210:five_prime_UTR 25.0%
!! TGTTATTTGGCATTGTAATA+TGG + tig0019103:1753-1772 Msa1370210:intron 25.0%
! AGACAATAATACTTCCTTTA+AGG + tig0019103:1778-1797 Msa1370210:intron 25.0%
! GACAATAATACTTCCTTTAA+GGG + tig0019103:1779-1798 Msa1370210:intron 25.0%
! ATGTGCTAATTATAAGAATG+TGG - tig0019103:2649-2668 Msa1370210:intergenic 25.0%
!! CTTTAATAGGTTTACACTTT+TGG + tig0019103:1547-1566 Msa1370210:five_prime_UTR 25.0%
!! TGTTATTTGGCATTGTAATA+TGG + tig0019103:1753-1772 Msa1370210:intron 25.0%
! AGACAATAATACTTCCTTTA+AGG + tig0019103:1778-1797 Msa1370210:intron 25.0%
! GACAATAATACTTCCTTTAA+GGG + tig0019103:1779-1798 Msa1370210:intron 25.0%
! ATGTGCTAATTATAAGAATG+TGG - tig0019103:2649-2668 Msa1370210:intergenic 25.0%
! TAATACTTCCTTTAAGGGAA+AGG + tig0019103:1784-1803 Msa1370210:intron 30.0%
AACTGTCTATTGTTGCTATA+AGG - tig0019103:1850-1869 Msa1370210:intergenic 30.0%
! TTTTGTTCCTTACGAAATTG+AGG + tig0019103:2170-2189 Msa1370210:intron 30.0%
TTTGTTCCTTACGAAATTGA+GGG + tig0019103:2171-2190 Msa1370210:intron 30.0%
TGTAACTTTCTGTCATTCAA+TGG + tig0019103:2228-2247 Msa1370210:intron 30.0%
TCAACACATCTCATTATGAA+AGG - tig0019103:2285-2304 Msa1370210:intergenic 30.0%
CATATGCAATAAATACGAAG+CGG - tig0019103:2413-2432 Msa1370210:intergenic 30.0%
! TCTCTCTTCTTTGTTTAGTT+TGG + tig0019103:2502-2521 Msa1370210:intron 30.0%
! TTTTTCTTCTAGATATGACG+AGG + tig0019103:2556-2575 Msa1370210:intron 30.0%
TTAATATCAGACACACTCAT+AGG - tig0019103:2607-2626 Msa1370210:intergenic 30.0%
AGTGTGTCTGATATTAAGTA+TGG + tig0019103:2610-2629 Msa1370210:intron 30.0%
ACTGCAATTTGTGAATGATT+CGG + tig0019103:2736-2755 Msa1370210:CDS 30.0%
TAACATGATATGAATGTCCA+CGG - tig0019103:2816-2835 Msa1370210:intergenic 30.0%
! TAATACTTCCTTTAAGGGAA+AGG + tig0019103:1784-1803 Msa1370210:intron 30.0%
AACTGTCTATTGTTGCTATA+AGG - tig0019103:1850-1869 Msa1370210:intergenic 30.0%
! TTTTGTTCCTTACGAAATTG+AGG + tig0019103:2170-2189 Msa1370210:intron 30.0%
TTTGTTCCTTACGAAATTGA+GGG + tig0019103:2171-2190 Msa1370210:intron 30.0%
TGTAACTTTCTGTCATTCAA+TGG + tig0019103:2228-2247 Msa1370210:intron 30.0%
TCAACACATCTCATTATGAA+AGG - tig0019103:2285-2304 Msa1370210:intergenic 30.0%
CATATGCAATAAATACGAAG+CGG - tig0019103:2413-2432 Msa1370210:intergenic 30.0%
! TCTCTCTTCTTTGTTTAGTT+TGG + tig0019103:2502-2521 Msa1370210:intron 30.0%
! TTTTTCTTCTAGATATGACG+AGG + tig0019103:2556-2575 Msa1370210:intron 30.0%
TTAATATCAGACACACTCAT+AGG - tig0019103:2607-2626 Msa1370210:intergenic 30.0%
AGTGTGTCTGATATTAAGTA+TGG + tig0019103:2610-2629 Msa1370210:intron 30.0%
ACTGCAATTTGTGAATGATT+CGG + tig0019103:2736-2755 Msa1370210:CDS 30.0%
TAACATGATATGAATGTCCA+CGG - tig0019103:2816-2835 Msa1370210:intergenic 30.0%
TTTCAGTTCCTTTCCCTTAA+AGG - tig0019103:1795-1814 Msa1370210:intergenic 35.0%
GTATTGTTCTACCAGAGATA+TGG + tig0019103:1882-1901 Msa1370210:intron 35.0%
AATGTCTTGCTTTACACTTC+AGG + tig0019103:1925-1944 Msa1370210:intron 35.0%
!! TGCAGTTTTATGCTATGCAA+AGG - tig0019103:2059-2078 Msa1370210:intergenic 35.0%
TGCATAGCATAAAACTGCAT+TGG + tig0019103:2060-2079 Msa1370210:intron 35.0%
ATAAGCAACAGTCCAAACAT+TGG - tig0019103:2130-2149 Msa1370210:intergenic 35.0%
TAGAAACCCTCAATTTCGTA+AGG - tig0019103:2180-2199 Msa1370210:intergenic 35.0%
AATTGAGGGTTTCTAAGTTG+AGG + tig0019103:2185-2204 Msa1370210:intron 35.0%
! ATTGAGGGTTTCTAAGTTGA+GGG + tig0019103:2186-2205 Msa1370210:intron 35.0%
CTGTCATTCAATGGCTAATA+TGG + tig0019103:2237-2256 Msa1370210:intron 35.0%
TCATTCAATGGCTAATATGG+TGG + tig0019103:2240-2259 Msa1370210:intron 35.0%
CATTCAATGGCTAATATGGT+GGG + tig0019103:2241-2260 Msa1370210:intron 35.0%
TTATTGCATATGCGTCTATG+AGG + tig0019103:2421-2440 Msa1370210:intron 35.0%
TGAACTTACAGGATTGTCTA+CGG - tig0019103:2789-2808 Msa1370210:intergenic 35.0%
TTTCAGTTCCTTTCCCTTAA+AGG - tig0019103:1795-1814 Msa1370210:intergenic 35.0%
GTATTGTTCTACCAGAGATA+TGG + tig0019103:1882-1901 Msa1370210:intron 35.0%
AATGTCTTGCTTTACACTTC+AGG + tig0019103:1925-1944 Msa1370210:intron 35.0%
!! TGCAGTTTTATGCTATGCAA+AGG - tig0019103:2059-2078 Msa1370210:intergenic 35.0%
TGCATAGCATAAAACTGCAT+TGG + tig0019103:2060-2079 Msa1370210:intron 35.0%
ATAAGCAACAGTCCAAACAT+TGG - tig0019103:2130-2149 Msa1370210:intergenic 35.0%
TAGAAACCCTCAATTTCGTA+AGG - tig0019103:2180-2199 Msa1370210:intergenic 35.0%
AATTGAGGGTTTCTAAGTTG+AGG + tig0019103:2185-2204 Msa1370210:intron 35.0%
! ATTGAGGGTTTCTAAGTTGA+GGG + tig0019103:2186-2205 Msa1370210:intron 35.0%
CTGTCATTCAATGGCTAATA+TGG + tig0019103:2237-2256 Msa1370210:intron 35.0%
TCATTCAATGGCTAATATGG+TGG + tig0019103:2240-2259 Msa1370210:intron 35.0%
CATTCAATGGCTAATATGGT+GGG + tig0019103:2241-2260 Msa1370210:intron 35.0%
TTATTGCATATGCGTCTATG+AGG + tig0019103:2421-2440 Msa1370210:intron 35.0%
TGAACTTACAGGATTGTCTA+CGG - tig0019103:2789-2808 Msa1370210:intergenic 35.0%
! GGTTCTCTTTTATCTTGCCA+TGG + tig0019103:1568-1587 Msa1370210:exon 40.0%
TTCTACCAGAGATATGGTCA+TGG + tig0019103:1888-1907 Msa1370210:intron 40.0%
TATGACCATGACCATATCTC+TGG - tig0019103:1896-1915 Msa1370210:intergenic 40.0%
ATTGAAGCCATACCAATCAG+CGG + tig0019103:1953-1972 Msa1370210:intron 40.0%
!! AGCATAAAACTGCATTGGCT+CGG + tig0019103:2065-2084 Msa1370210:intron 40.0%
! CTGTACAAAGTGCCAATGTT+TGG + tig0019103:2115-2134 Msa1370210:intron 40.0%
TCTTCTAGATATGACGAGGT+TGG + tig0019103:2560-2579 Msa1370210:intron 40.0%
! GGTTCTCTTTTATCTTGCCA+TGG + tig0019103:1568-1587 Msa1370210:exon 40.0%
TTCTACCAGAGATATGGTCA+TGG + tig0019103:1888-1907 Msa1370210:intron 40.0%
TATGACCATGACCATATCTC+TGG - tig0019103:1896-1915 Msa1370210:intergenic 40.0%
ATTGAAGCCATACCAATCAG+CGG + tig0019103:1953-1972 Msa1370210:intron 40.0%
!! AGCATAAAACTGCATTGGCT+CGG + tig0019103:2065-2084 Msa1370210:intron 40.0%
! CTGTACAAAGTGCCAATGTT+TGG + tig0019103:2115-2134 Msa1370210:intron 40.0%
TCTTCTAGATATGACGAGGT+TGG + tig0019103:2560-2579 Msa1370210:intron 40.0%
! CTCTTTTATCTTGCCATGGC+CGG + tig0019103:1572-1591 Msa1370210:exon 45.0%
! TCTTTTATCTTGCCATGGCC+GGG + tig0019103:1573-1592 Msa1370210:exon 45.0%
GGAGCTGTGACTGTTAATTC+TGG + tig0019103:2344-2363 Msa1370210:intron 45.0%
CATATGCGTCTATGAGGCAA+TGG + tig0019103:2427-2446 Msa1370210:intron 45.0%
TCTACGGTGTGTGAACTTAC+AGG - tig0019103:2773-2792 Msa1370210:intergenic 45.0%
! CTCTTTTATCTTGCCATGGC+CGG + tig0019103:1572-1591 Msa1370210:exon 45.0%
! TCTTTTATCTTGCCATGGCC+GGG + tig0019103:1573-1592 Msa1370210:exon 45.0%
GGAGCTGTGACTGTTAATTC+TGG + tig0019103:2344-2363 Msa1370210:intron 45.0%
CATATGCGTCTATGAGGCAA+TGG + tig0019103:2427-2446 Msa1370210:intron 45.0%
TCTACGGTGTGTGAACTTAC+AGG - tig0019103:2773-2792 Msa1370210:intergenic 45.0%
! CTTTTATCTTGCCATGGCCG+GGG + tig0019103:1574-1593 Msa1370210:exon 50.0%
GAACTGAGCAGTTTCAACCC+CGG - tig0019103:1594-1613 Msa1370210:intergenic 50.0%
GGGTTGAAACTGCTCAGTTC+AGG + tig0019103:1594-1613 Msa1370210:CDS 50.0%
! TTAGCAGCCGCTGATTGGTA+TGG - tig0019103:1963-1982 Msa1370210:intergenic 50.0%
ATCTGTTAGCAGCCGCTGAT+TGG - tig0019103:1968-1987 Msa1370210:intergenic 50.0%
TCAGCGGCTGCTAACAGATA+AGG + tig0019103:1969-1988 Msa1370210:intron 50.0%
CGGCTGCTAACAGATAAGGA+TGG + tig0019103:1973-1992 Msa1370210:intron 50.0%
CGAGGTTGGCATCAACTAGA+TGG + tig0019103:2574-2593 Msa1370210:intron 50.0%
TCCTGTAAGTTCACACACCG+TGG + tig0019103:2769-2788 Msa1370210:CDS 50.0%
TCCACGGTGTGTGAACTTAC+AGG - tig0019103:2800-2819 Msa1370210:intergenic 50.0%
! CTTTTATCTTGCCATGGCCG+GGG + tig0019103:1574-1593 Msa1370210:exon 50.0%
GAACTGAGCAGTTTCAACCC+CGG - tig0019103:1594-1613 Msa1370210:intergenic 50.0%
GGGTTGAAACTGCTCAGTTC+AGG + tig0019103:1594-1613 Msa1370210:CDS 50.0%
! TTAGCAGCCGCTGATTGGTA+TGG - tig0019103:1963-1982 Msa1370210:intergenic 50.0%
ATCTGTTAGCAGCCGCTGAT+TGG - tig0019103:1968-1987 Msa1370210:intergenic 50.0%
TCAGCGGCTGCTAACAGATA+AGG + tig0019103:1969-1988 Msa1370210:intron 50.0%
CGGCTGCTAACAGATAAGGA+TGG + tig0019103:1973-1992 Msa1370210:intron 50.0%
CGAGGTTGGCATCAACTAGA+TGG + tig0019103:2574-2593 Msa1370210:intron 50.0%
TCCTGTAAGTTCACACACCG+TGG + tig0019103:2769-2788 Msa1370210:CDS 50.0%
TCCACGGTGTGTGAACTTAC+AGG - tig0019103:2800-2819 Msa1370210:intergenic 50.0%
AGCAGTTTCAACCCCGGCCA+TGG - tig0019103:1588-1607 Msa1370210:intergenic 60.0%
AGCAGTTTCAACCCCGGCCA+TGG - tig0019103:1588-1607 Msa1370210:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0019103 gene 1490 2841 1490 ID=Msa1370210;Name=Msa1370210
tig0019103 mRNA 1490 2841 1490 ID=Msa1370210-mRNA-1;Parent=Msa1370210;Name=Msa1370210-mRNA-1;_AED=0.55;_eAED=0.55;_QI=97|0|0.5|0.5|1|1|2|0|46
tig0019103 exon 1490 1615 1490 ID=Msa1370210-mRNA-1:exon:1585;Parent=Msa1370210-mRNA-1
tig0019103 exon 2730 2841 2730 ID=Msa1370210-mRNA-1:exon:1586;Parent=Msa1370210-mRNA-1
tig0019103 five_prime_UTR 1490 1586 1490 ID=Msa1370210-mRNA-1:five_prime_utr;Parent=Msa1370210-mRNA-1
tig0019103 CDS 1587 1615 1587 ID=Msa1370210-mRNA-1:cds;Parent=Msa1370210-mRNA-1
tig0019103 CDS 2730 2841 2730 ID=Msa1370210-mRNA-1:cds;Parent=Msa1370210-mRNA-1
Gene Sequence

>Msa1370210

ATGGCCGGGGTTGAAACTGCTCAGTTCAGCTATGCACTGCAATTTGTGAATGATTCGGCATGCATTAATCCTGTAAGTTCACACACCGTAGACAATCCTGTAAGTTCACACACCGTGGACATTCATATCATGTTAATTTGA

Protein sequence

>Msa1370210

MAGVETAQFSYALQFVNDSACINPVSSHTVDNPVSSHTVDIHIMLI*