Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1411100 | sp|Q8L7E9|MCAC1_ARATH | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.49e-69 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1411100 | G7LB65 | 94.400 | 125 | 7 | 0 | 1 | 125 | 1 | 125 | 8.23e-78 | 245 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007440 | Msa1411100 | 0.810100 | 1.363083e-50 | -8.615850e-47 |
| Msa0015140 | Msa1411100 | 0.805214 | 1.487548e-49 | -8.615850e-47 |
| Msa0033980 | Msa1411100 | 0.827339 | 1.652402e-54 | -8.615850e-47 |
| Msa0070170 | Msa1411100 | 0.833393 | 5.471419e-56 | -8.615850e-47 |
| Msa0081930 | Msa1411100 | 0.811141 | 8.121784e-51 | -8.615850e-47 |
| Msa0087800 | Msa1411100 | 0.801412 | 9.113370e-49 | -8.615850e-47 |
| Msa1382790 | Msa1411100 | 0.805771 | 1.136555e-49 | -8.615850e-47 |
| Msa1407190 | Msa1411100 | 0.802649 | 5.074236e-49 | -8.615850e-47 |
| Msa1411100 | Msa1437270 | 0.803833 | 2.887189e-49 | -8.615850e-47 |
| Msa0638350 | Msa1411100 | 0.852470 | 4.521567e-61 | -8.615850e-47 |
| Msa0682090 | Msa1411100 | 0.834359 | 3.137673e-56 | -8.615850e-47 |
| Msa0682140 | Msa1411100 | 0.832347 | 9.954416e-56 | -8.615850e-47 |
| Msa0688390 | Msa1411100 | 0.805106 | 1.566791e-49 | -8.615850e-47 |
| Msa0706400 | Msa1411100 | 0.803295 | 3.732180e-49 | -8.615850e-47 |
| Msa0822500 | Msa1411100 | 0.801728 | 7.852198e-49 | -8.615850e-47 |
| Msa0831850 | Msa1411100 | 0.806142 | 9.497114e-50 | -8.615850e-47 |
| Msa0138020 | Msa1411100 | 0.800061 | 1.719696e-48 | -8.615850e-47 |
| Msa0185510 | Msa1411100 | 0.810367 | 1.193835e-50 | -8.615850e-47 |
| Msa0217290 | Msa1411100 | 0.806630 | 7.493692e-50 | -8.615850e-47 |
| Msa1286610 | Msa1411100 | 0.805753 | 1.146672e-49 | -8.615850e-47 |
| Msa1287460 | Msa1411100 | 0.805615 | 1.225729e-49 | -8.615850e-47 |
| Msa1306560 | Msa1411100 | 0.828183 | 1.035488e-54 | -8.615850e-47 |
| Msa0383800 | Msa1411100 | 0.800705 | 1.271696e-48 | -8.615850e-47 |
| Msa0434180 | Msa1411100 | 0.800233 | 1.586485e-48 | -8.615850e-47 |
| Msa0437400 | Msa1411100 | 0.813147 | 2.965251e-51 | -8.615850e-47 |
| Msa0481750 | Msa1411100 | 0.805016 | 1.636225e-49 | -8.615850e-47 |
| Msa1001390 | Msa1411100 | 0.804032 | 2.623842e-49 | -8.615850e-47 |
| Msa1005210 | Msa1411100 | 0.807493 | 4.920872e-50 | -8.615850e-47 |
| Msa1043250 | Msa1411100 | -0.804668 | 1.934091e-49 | -8.615850e-47 |
| Msa1064250 | Msa1411100 | 0.849684 | 2.760518e-60 | -8.615850e-47 |
| Msa1080640 | Msa1411100 | 0.807369 | 5.227422e-50 | -8.615850e-47 |
| Msa0241140 | Msa1411100 | 0.802703 | 4.945932e-49 | -8.615850e-47 |
| Msa0265380 | Msa1411100 | 0.809984 | 1.443713e-50 | -8.615850e-47 |
| Msa0303360 | Msa1411100 | 0.817594 | 3.040693e-52 | -8.615850e-47 |
| Msa0309770 | Msa1411100 | 0.806343 | 8.615441e-50 | -8.615850e-47 |
| Msa0318980 | Msa1411100 | 0.801981 | 6.967536e-49 | -8.615850e-47 |
| Msa0361140 | Msa1411100 | 0.961130 | 2.400454e-119 | -8.615850e-47 |
| Msa1111870 | Msa1411100 | 0.809132 | 2.200556e-50 | -8.615850e-47 |
| Msa1126450 | Msa1411100 | 0.813536 | 2.435717e-51 | -8.615850e-47 |
| Msa1128060 | Msa1411100 | 0.809126 | 2.207827e-50 | -8.615850e-47 |
| Msa1163540 | Msa1411100 | 0.842997 | 1.836721e-58 | -8.615850e-47 |
| Msa0546570 | Msa1411100 | 0.832186 | 1.091227e-55 | -8.615850e-47 |
| Msa0584860 | Msa1411100 | 0.804705 | 1.899858e-49 | -8.615850e-47 |
| Msa0901320 | Msa1411100 | 0.809251 | 2.074785e-50 | -8.615850e-47 |
| Msa0969810 | Msa1411100 | 0.801361 | 9.336925e-49 | -8.615850e-47 |
| Msa0973600 | Msa1411100 | 0.800195 | 1.614753e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1411100 | MtrunA17_Chr8g0371431 | 94.400 | 125 | 7 | 0 | 1 | 125 | 1 | 125 | 1.58e-81 | 245 |
| Msa1411100 | MtrunA17_Chr5g0405841 | 82.927 | 123 | 21 | 0 | 3 | 125 | 2 | 124 | 1.61e-71 | 219 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1411100 | AT4G35920.1 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.56e-70 | 216 |
| Msa1411100 | AT4G35920.5 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.56e-70 | 216 |
| Msa1411100 | AT4G35920.4 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.56e-70 | 216 |
| Msa1411100 | AT4G35920.2 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.56e-70 | 216 |
| Msa1411100 | AT4G35920.3 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.56e-70 | 216 |
| Msa1411100 | AT2G17780.4 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 8.16e-59 | 184 |
| Msa1411100 | AT2G17780.8 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 4.03e-58 | 184 |
| Msa1411100 | AT2G17780.7 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.93e-58 | 184 |
| Msa1411100 | AT2G17780.6 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.93e-58 | 184 |
| Msa1411100 | AT2G17780.10 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.93e-58 | 184 |
| Msa1411100 | AT2G17780.5 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.93e-58 | 184 |
| Msa1411100 | AT2G17780.1 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.93e-58 | 184 |
| Msa1411100 | AT2G17780.3 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 7.64e-58 | 184 |
| Msa1411100 | AT2G17780.9 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 7.64e-58 | 184 |
| Msa1411100 | AT2G17780.2 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 8.29e-58 | 184 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGAGGCTCTCGGGTTTC+AGG | 0.140533 | tig0023720:+69156 | None:intergenic |
| CTGCATCTAAGCCTATTAAT+TGG | 0.182088 | tig0023720:+69316 | None:intergenic |
| TTTGAGAAGGTCTTCTATTT+TGG | 0.212238 | tig0023720:-69118 | Msa1411100:CDS |
| GGAACATTGTTTATCAATTT+AGG | 0.249469 | tig0023720:-69047 | Msa1411100:CDS |
| GCAAATGTAGCCCAATTAAT+AGG | 0.272486 | tig0023720:-69327 | Msa1411100:CDS |
| GCATCTTCATGGGAACAATT+TGG | 0.278027 | tig0023720:-69357 | Msa1411100:CDS |
| GCACAACATCTTAAGTTGAT+TGG | 0.319124 | tig0023720:-69222 | Msa1411100:CDS |
| TAGATGCAGTAAAGTTGATT+AGG | 0.362979 | tig0023720:-69302 | Msa1411100:CDS |
| CACAACATCTTAAGTTGATT+GGG | 0.378407 | tig0023720:-69221 | Msa1411100:CDS |
| CTAGTTGTTCTAGAGGCTCT+CGG | 0.379192 | tig0023720:+69148 | None:intergenic |
| GATATAGAATGGCATCTTCA+TGG | 0.416346 | tig0023720:-69368 | None:intergenic |
| TGCATCTAAGCCTATTAATT+GGG | 0.419039 | tig0023720:+69317 | None:intergenic |
| CTAGAGGCTCTCGGGTTTCA+GGG | 0.434211 | tig0023720:+69157 | None:intergenic |
| CTATCTTTATTTGCTCGCCA+TGG | 0.439513 | tig0023720:-69073 | Msa1411100:CDS |
| TATCTTTATTTGCTCGCCAT+GGG | 0.488601 | tig0023720:-69072 | Msa1411100:CDS |
| TAGTTGTTCTAGAGGCTCTC+GGG | 0.496041 | tig0023720:+69149 | None:intergenic |
| TTTATTTGCTCGCCATGGGA+TGG | 0.519477 | tig0023720:-69068 | Msa1411100:CDS |
| ATATAGAATGGCATCTTCAT+GGG | 0.542232 | tig0023720:-69367 | None:intergenic |
| GCATCTTCTAGTTGTTCTAG+AGG | 0.556880 | tig0023720:+69141 | None:intergenic |
| CATTGTTTATCAATTTAGGA+AGG | 0.567874 | tig0023720:-69043 | Msa1411100:CDS |
| ATAAACAATGTTCCATCCCA+TGG | 0.583807 | tig0023720:+69056 | None:intergenic |
| AACTAGAAGATGCTTTGAGA+AGG | 0.613004 | tig0023720:-69131 | Msa1411100:CDS |
| CGAAAGCAGCAAATACAGCA+AGG | 0.638455 | tig0023720:-69272 | Msa1411100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GGAACATTGTTTATCAATTT+AGG | - | tig0023720:69315-69334 | Msa1411100:CDS | 25.0% |
| ! | CATTGTTTATCAATTTAGGA+AGG | - | tig0023720:69319-69338 | Msa1411100:CDS | 25.0% |
| TGCATCTAAGCCTATTAATT+GGG | + | tig0023720:69048-69067 | None:intergenic | 30.0% | |
| ! | TAGATGCAGTAAAGTTGATT+AGG | - | tig0023720:69060-69079 | Msa1411100:CDS | 30.0% |
| !! | CACAACATCTTAAGTTGATT+GGG | - | tig0023720:69141-69160 | Msa1411100:CDS | 30.0% |
| ! | TTTGAGAAGGTCTTCTATTT+TGG | - | tig0023720:69244-69263 | Msa1411100:CDS | 30.0% |
| !!! | TATTTTGGTCAATAGTTGTC+AGG | - | tig0023720:69259-69278 | Msa1411100:CDS | 30.0% |
| GCAAATGTAGCCCAATTAAT+AGG | - | tig0023720:69035-69054 | Msa1411100:CDS | 35.0% | |
| CTGCATCTAAGCCTATTAAT+TGG | + | tig0023720:69049-69068 | None:intergenic | 35.0% | |
| !! | GCACAACATCTTAAGTTGAT+TGG | - | tig0023720:69140-69159 | Msa1411100:CDS | 35.0% |
| !! | AACTAGAAGATGCTTTGAGA+AGG | - | tig0023720:69231-69250 | Msa1411100:CDS | 35.0% |
| ! | TATCTTTATTTGCTCGCCAT+GGG | - | tig0023720:69290-69309 | Msa1411100:CDS | 35.0% |
| ATAAACAATGTTCCATCCCA+TGG | + | tig0023720:69309-69328 | None:intergenic | 35.0% | |
| GCATCTTCATGGGAACAATT+TGG | - | tig0023720:69005-69024 | Msa1411100:CDS | 40.0% | |
| ! | GCATCTTCTAGTTGTTCTAG+AGG | + | tig0023720:69224-69243 | None:intergenic | 40.0% |
| ! | CTATCTTTATTTGCTCGCCA+TGG | - | tig0023720:69289-69308 | Msa1411100:CDS | 40.0% |
| CGAAAGCAGCAAATACAGCA+AGG | - | tig0023720:69090-69109 | Msa1411100:CDS | 45.0% | |
| !! | TAGTTGTTCTAGAGGCTCTC+GGG | + | tig0023720:69216-69235 | None:intergenic | 45.0% |
| !! | CTAGTTGTTCTAGAGGCTCT+CGG | + | tig0023720:69217-69236 | None:intergenic | 45.0% |
| TTTATTTGCTCGCCATGGGA+TGG | - | tig0023720:69294-69313 | Msa1411100:CDS | 45.0% | |
| CTAGAGGCTCTCGGGTTTCA+GGG | + | tig0023720:69208-69227 | None:intergenic | 55.0% | |
| TCTAGAGGCTCTCGGGTTTC+AGG | + | tig0023720:69209-69228 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023720 | gene | 69002 | 69382 | 69002 | ID=Msa1411100;Name=Msa1411100 |
| tig0023720 | mRNA | 69002 | 69382 | 69002 | ID=Msa1411100-mRNA-1;Parent=Msa1411100;Name=Msa1411100-mRNA-1;_AED=0.47;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|126 |
| tig0023720 | exon | 69002 | 69382 | 69002 | ID=Msa1411100-mRNA-1:exon:19913;Parent=Msa1411100-mRNA-1 |
| tig0023720 | CDS | 69002 | 69382 | 69002 | ID=Msa1411100-mRNA-1:cds;Parent=Msa1411100-mRNA-1 |
| Gene Sequence |
| Protein sequence |
