AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0033980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033980 MtrunA17_Chr8g0345501 65.169 89 31 0 24 112 408 496 7.13e-34 123
Msa0033980 MtrunA17_Chr2g0313001 72.500 40 11 0 7 46 1 40 2.43e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTATCTCGAGCCAAATT+TGG 0.138676 1_1:+61856558 None:intergenic
TTACCTCTTTATCCCAAATT+TGG 0.184345 1_1:-61856571 None:intergenic
CCATCACCATCTGGAATTAT+TGG 0.275118 1_1:+61857700 None:intergenic
AAGAAATTGCTCACATTATA+TGG 0.286562 1_1:-61857163 Msa0033980:CDS
TGATTTCTTTGACTCATTCA+TGG 0.333269 1_1:-61857084 Msa0033980:intron
CCGTCATGGAAGCAATTATC+AGG 0.356730 1_1:-61857119 Msa0033980:CDS
AACTATCTCGAGCCAAATTT+GGG 0.363047 1_1:+61856559 None:intergenic
GTAGATTGAGTCAAAGTTGA+AGG 0.423072 1_1:-61856859 Msa0033980:exon
ACTTGTCATGATGCATGTTA+TGG 0.428964 1_1:+61856761 None:intergenic
AATGTGTACATTTCAAGTTA+TGG 0.444448 1_1:-61857782 Msa0033980:CDS
CAAACTCACCCATCACCATC+TGG 0.453372 1_1:+61857691 None:intergenic
CTCAGTTGATGCAAGAAGTT+CGG 0.458850 1_1:-61856886 Msa0033980:CDS
TACAAGGCTTAAGCAACAAT+TGG 0.471347 1_1:-61856829 Msa0033980:three_prime_UTR
TACATGGCAACCAGAGCATA+AGG 0.485592 1_1:-61856954 Msa0033980:CDS
CTAACATGGCTAGCTGAAGA+TGG 0.497267 1_1:+61857748 None:intergenic
TGCACATAGTAGTTCAGTTG+TGG 0.523431 1_1:-61857825 Msa0033980:five_prime_UTR
CCAATAATTCCAGATGGTGA+TGG 0.531511 1_1:-61857700 Msa0033980:CDS
GAGCCAAATTTGGGATAAAG+AGG 0.548288 1_1:+61856568 None:intergenic
CAATAATTCCAGATGGTGAT+GGG 0.552654 1_1:-61857699 Msa0033980:intron
CAACGGTGTCTCTACTAACA+TGG 0.557176 1_1:+61857734 None:intergenic
CCTGATAATTGCTTCCATGA+CGG 0.572248 1_1:+61857119 None:intergenic
CATAGTAGTTCAGTTGTGGT+TGG 0.585520 1_1:-61857821 Msa0033980:five_prime_UTR
TAGAAGAGGGAAAACCGTCA+TGG 0.601838 1_1:-61857133 Msa0033980:CDS
TATATGGTCTCAATTAGAAG+AGG 0.602911 1_1:-61857147 Msa0033980:CDS
TTTCTTTAACTACCAATGTG+TGG 0.607987 1_1:+61857180 None:intergenic
AAAATACCAATAATTCCAGA+TGG 0.610541 1_1:-61857706 Msa0033980:CDS
AGTTGAAGGAGGCAACTACA+AGG 0.610652 1_1:-61856845 Msa0033980:exon
ATATGGTCTCAATTAGAAGA+GGG 0.627134 1_1:-61857146 Msa0033980:CDS
CTTGAAATGTACACATTGCG+AGG 0.634227 1_1:+61857788 None:intergenic
CAGGTTTGATCACCACACAT+TGG 0.634316 1_1:-61857192 Msa0033980:CDS
GATTGAGTCAAAGTTGAAGG+AGG 0.637853 1_1:-61856856 Msa0033980:exon
ATGAGTCAAAGAAATCATCT+CGG 0.652827 1_1:+61857090 None:intergenic
CATGTTAGTAGAGACACCGT+TGG 0.707519 1_1:-61857733 Msa0033980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAAATAATCATTATTATAT+GGG + chr1_1:61856800-61856819 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG - chr1_1:61856812-61856831 Msa0033980:three_prime_UTR 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG - chr1_1:61857127-61857146 Msa0033980:CDS 15.0%
!! CCAAATAATCATTATTATAT+GGG + chr1_1:61856800-61856819 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG - chr1_1:61856812-61856831 Msa0033980:three_prime_UTR 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG - chr1_1:61857127-61857146 Msa0033980:CDS 15.0%
!! CCCATATAATAATGATTATT+TGG - chr1_1:61856797-61856816 Msa0033980:three_prime_UTR 20.0%
!! GCCAAATAATCATTATTATA+TGG + chr1_1:61856801-61856820 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG - chr1_1:61857490-61857509 Msa0033980:intron 20.0%
!! CCCATATAATAATGATTATT+TGG - chr1_1:61856797-61856816 Msa0033980:three_prime_UTR 20.0%
!! GCCAAATAATCATTATTATA+TGG + chr1_1:61856801-61856820 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG - chr1_1:61857490-61857509 Msa0033980:intron 20.0%
!!! AATGTGTACATTTCAAGTTA+TGG - chr1_1:61856678-61856697 Msa0033980:three_prime_UTR 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG + chr1_1:61856746-61856765 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG - chr1_1:61856754-61856773 Msa0033980:three_prime_UTR 25.0%
!! TTGTGGTATTATGATTTTAG+TGG - chr1_1:61856895-61856914 Msa0033980:CDS 25.0%
!! TTTTCGTGTACATTATATTC+TGG - chr1_1:61856944-61856963 Msa0033980:CDS 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG - chr1_1:61857133-61857152 Msa0033980:CDS 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG - chr1_1:61857171-61857190 Msa0033980:CDS 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG - chr1_1:61857249-61857268 Msa0033980:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG - chr1_1:61857297-61857316 Msa0033980:intron 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG + chr1_1:61857440-61857459 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG - chr1_1:61857535-61857554 Msa0033980:intron 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG - chr1_1:61857536-61857555 Msa0033980:intron 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG - chr1_1:61857537-61857556 Msa0033980:intron 25.0%
! AGCATATGCACAAAAAAAAA+TGG + chr1_1:61857856-61857875 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG - chr1_1:61857861-61857880 Msa0033980:five_prime_UTR 25.0%
!!! AATGTGTACATTTCAAGTTA+TGG - chr1_1:61856678-61856697 Msa0033980:three_prime_UTR 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG + chr1_1:61856746-61856765 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG - chr1_1:61856754-61856773 Msa0033980:three_prime_UTR 25.0%
!! TTGTGGTATTATGATTTTAG+TGG - chr1_1:61856895-61856914 Msa0033980:CDS 25.0%
!! TTTTCGTGTACATTATATTC+TGG - chr1_1:61856944-61856963 Msa0033980:CDS 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG - chr1_1:61857133-61857152 Msa0033980:CDS 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG - chr1_1:61857171-61857190 Msa0033980:CDS 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG - chr1_1:61857249-61857268 Msa0033980:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG - chr1_1:61857297-61857316 Msa0033980:intron 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG + chr1_1:61857440-61857459 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG - chr1_1:61857535-61857554 Msa0033980:intron 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG - chr1_1:61857536-61857555 Msa0033980:intron 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG - chr1_1:61857537-61857556 Msa0033980:intron 25.0%
! AGCATATGCACAAAAAAAAA+TGG + chr1_1:61857856-61857875 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG - chr1_1:61857861-61857880 Msa0033980:five_prime_UTR 25.0%
! GATTCTGTTAACTTTCCTTT+TGG - chr1_1:61856844-61856863 Msa0033980:exon 30.0%
! TTTTCCTGAGAAAATATTGG+TGG - chr1_1:61857174-61857193 Msa0033980:CDS 30.0%
CAAGTAAAGTCTTAACTCAA+AGG + chr1_1:61857232-61857251 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG + chr1_1:61857283-61857302 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG - chr1_1:61857313-61857332 Msa0033980:intron 30.0%
ATATGGTCTCAATTAGAAGA+GGG - chr1_1:61857314-61857333 Msa0033980:intron 30.0%
ATGAGTCAAAGAAATCATCT+CGG + chr1_1:61857373-61857392 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG - chr1_1:61857376-61857395 Msa0033980:intron 30.0%
! GATTCTGTTAACTTTCCTTT+TGG - chr1_1:61856844-61856863 Msa0033980:exon 30.0%
! TTTTCCTGAGAAAATATTGG+TGG - chr1_1:61857174-61857193 Msa0033980:CDS 30.0%
CAAGTAAAGTCTTAACTCAA+AGG + chr1_1:61857232-61857251 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG + chr1_1:61857283-61857302 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG - chr1_1:61857313-61857332 Msa0033980:intron 30.0%
ATATGGTCTCAATTAGAAGA+GGG - chr1_1:61857314-61857333 Msa0033980:intron 30.0%
ATGAGTCAAAGAAATCATCT+CGG + chr1_1:61857373-61857392 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG - chr1_1:61857376-61857395 Msa0033980:intron 30.0%
CAATAATTCCAGATGGTGAT+GGG - chr1_1:61856761-61856780 Msa0033980:three_prime_UTR 35.0%
! GCATTGTTACTTGATCCAAA+AGG + chr1_1:61856862-61856881 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG - chr1_1:61857030-61857049 Msa0033980:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG - chr1_1:61857601-61857620 Msa0033980:intron 35.0%
TACAAGGCTTAAGCAACAAT+TGG - chr1_1:61857631-61857650 Msa0033980:intron 35.0%
ACTTGTCATGATGCATGTTA+TGG + chr1_1:61857702-61857721 None:intergenic 35.0%
CAATAATTCCAGATGGTGAT+GGG - chr1_1:61856761-61856780 Msa0033980:three_prime_UTR 35.0%
! GCATTGTTACTTGATCCAAA+AGG + chr1_1:61856862-61856881 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG - chr1_1:61857030-61857049 Msa0033980:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG - chr1_1:61857601-61857620 Msa0033980:intron 35.0%
TACAAGGCTTAAGCAACAAT+TGG - chr1_1:61857631-61857650 Msa0033980:intron 35.0%
ACTTGTCATGATGCATGTTA+TGG + chr1_1:61857702-61857721 None:intergenic 35.0%
TGCACATAGTAGTTCAGTTG+TGG - chr1_1:61856635-61856654 Msa0033980:three_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG - chr1_1:61856639-61856658 Msa0033980:three_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG + chr1_1:61856675-61856694 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG + chr1_1:61856763-61856782 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG - chr1_1:61856760-61856779 Msa0033980:three_prime_UTR 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG - chr1_1:61857140-61857159 Msa0033980:CDS 40.0%
! TGCACCACCAATATTTTCTC+AGG + chr1_1:61857181-61857200 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG - chr1_1:61857196-61857215 Msa0033980:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG + chr1_1:61857344-61857363 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG - chr1_1:61857574-61857593 Msa0033980:intron 40.0%
GATTGAGTCAAAGTTGAAGG+AGG - chr1_1:61857604-61857623 Msa0033980:intron 40.0%
TGCACATAGTAGTTCAGTTG+TGG - chr1_1:61856635-61856654 Msa0033980:three_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG - chr1_1:61856639-61856658 Msa0033980:three_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG + chr1_1:61856675-61856694 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG + chr1_1:61856763-61856782 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG - chr1_1:61856760-61856779 Msa0033980:three_prime_UTR 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG - chr1_1:61857140-61857159 Msa0033980:CDS 40.0%
! TGCACCACCAATATTTTCTC+AGG + chr1_1:61857181-61857200 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG - chr1_1:61857196-61857215 Msa0033980:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG + chr1_1:61857344-61857363 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG - chr1_1:61857574-61857593 Msa0033980:intron 40.0%
GATTGAGTCAAAGTTGAAGG+AGG - chr1_1:61857604-61857623 Msa0033980:intron 40.0%
! CTAACATGGCTAGCTGAAGA+TGG + chr1_1:61856715-61856734 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG + chr1_1:61856729-61856748 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG - chr1_1:61856727-61856746 Msa0033980:three_prime_UTR 45.0%
ATGCAAGACTGTTGTGCTTG+TGG - chr1_1:61856878-61856897 Msa0033980:CDS 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG - chr1_1:61857144-61857163 Msa0033980:CDS 45.0%
CAGGTTTGATCACCACACAT+TGG - chr1_1:61857268-61857287 Msa0033980:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG - chr1_1:61857327-61857346 Msa0033980:intron 45.0%
CCGTCATGGAAGCAATTATC+AGG - chr1_1:61857341-61857360 Msa0033980:intron 45.0%
TACATGGCAACCAGAGCATA+AGG - chr1_1:61857506-61857525 Msa0033980:intron 45.0%
! CGCACTTTTACCTTATGCTC+TGG + chr1_1:61857519-61857538 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG - chr1_1:61857615-61857634 Msa0033980:intron 45.0%
! CTAACATGGCTAGCTGAAGA+TGG + chr1_1:61856715-61856734 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG + chr1_1:61856729-61856748 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG - chr1_1:61856727-61856746 Msa0033980:three_prime_UTR 45.0%
ATGCAAGACTGTTGTGCTTG+TGG - chr1_1:61856878-61856897 Msa0033980:CDS 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG - chr1_1:61857144-61857163 Msa0033980:CDS 45.0%
CAGGTTTGATCACCACACAT+TGG - chr1_1:61857268-61857287 Msa0033980:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG - chr1_1:61857327-61857346 Msa0033980:intron 45.0%
CCGTCATGGAAGCAATTATC+AGG - chr1_1:61857341-61857360 Msa0033980:intron 45.0%
TACATGGCAACCAGAGCATA+AGG - chr1_1:61857506-61857525 Msa0033980:intron 45.0%
! CGCACTTTTACCTTATGCTC+TGG + chr1_1:61857519-61857538 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG - chr1_1:61857615-61857634 Msa0033980:intron 45.0%
CAAACTCACCCATCACCATC+TGG + chr1_1:61856772-61856791 None:intergenic 50.0%
CAAACTCACCCATCACCATC+TGG + chr1_1:61856772-61856791 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 61856573 61857909 61856573 ID=Msa0033980;Name=Msa0033980
chr1_1 mRNA 61856573 61857909 61856573 ID=Msa0033980-mRNA-1;Parent=Msa0033980;Name=Msa0033980-mRNA-1;_AED=0.28;_eAED=0.28;_QI=106|1|1|1|1|1|3|289|117
chr1_1 exon 61856573 61856984 61856573 ID=Msa0033980-mRNA-1:exon:19397;Parent=Msa0033980-mRNA-1
chr1_1 exon 61857085 61857211 61857085 ID=Msa0033980-mRNA-1:exon:19396;Parent=Msa0033980-mRNA-1
chr1_1 exon 61857700 61857909 61857700 ID=Msa0033980-mRNA-1:exon:19395;Parent=Msa0033980-mRNA-1
chr1_1 five_prime_UTR 61857804 61857909 61857804 ID=Msa0033980-mRNA-1:five_prime_utr;Parent=Msa0033980-mRNA-1
chr1_1 CDS 61857700 61857803 61857700 ID=Msa0033980-mRNA-1:cds;Parent=Msa0033980-mRNA-1
chr1_1 CDS 61857085 61857211 61857085 ID=Msa0033980-mRNA-1:cds;Parent=Msa0033980-mRNA-1
chr1_1 CDS 61856862 61856984 61856862 ID=Msa0033980-mRNA-1:cds;Parent=Msa0033980-mRNA-1
chr1_1 three_prime_UTR 61856573 61856861 61856573 ID=Msa0033980-mRNA-1:three_prime_utr;Parent=Msa0033980-mRNA-1
Gene Sequence

>Msa0033980

ATGTGTACATTTCAAGTTATGGTCAATAGAGCTCCATCTTCAGCTAGCCATGTTAGTAGAGACACCGTTGGTACTAAAATACCAATAATTCCAGATGGTGATGGGTTTGATCACCACACATTGGTAGTTAAAGAAATTGCTCACATTATATGGTCTCAATTAGAAGAGGGAAAACCGTCATGGAAGCAATTATCAGGAAATCACCGAGATGATTTCTTTGACTCATTCATGAAAAAGTTTACATGGCAACCAGAGCATAAGGTAAAAGTGCGAAAAAACTTTGAAATAAGGGGTGCAGCTAAGATGTCTCAGTTGATGCAAGAAGTTCGGAAAAGTAGATTGAGTCAAAGTTGA

Protein sequence

>Msa0033980

MCTFQVMVNRAPSSASHVSRDTVGTKIPIIPDGDGFDHHTLVVKEIAHIIWSQLEEGKPSWKQLSGNHRDDFFDSFMKKFTWQPEHKVKVRKNFEIRGAAKMSQLMQEVRKSRLSQS*