AlfalfaGEDB | MS.gene050493

M. sativa cultivar XinJiangDaYe / MS.gene050493

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050493.t1	XP_003626377.2	97.2	176	5	0	1	176	1	176	3.00E-95	357.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050493.t1	O34569	29.1	175	116	3	4	172	5	177	1.4e-13	77.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050493.t1	G7L0S4	97.2	176	5	0	1	176	1	176	2.1e-95	357.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene050493.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050493	MS.gene056761	0.810177	1.31E-50	-1.69E-46
MS.gene050493	MS.gene059022	-0.814773	1.30E-51	-1.69E-46
MS.gene050493	MS.gene060337	0.803062	4.17E-49	-1.69E-46
MS.gene050493	MS.gene06299	0.807912	4.01E-50	-1.69E-46
MS.gene050493	MS.gene071810	0.819808	9.56E-53	-1.69E-46
MS.gene050493	MS.gene22826	-0.80166	8.11E-49	-1.69E-46
MS.gene050493	MS.gene22888	0.905783	2.98E-80	-1.69E-46
MS.gene050493	MS.gene24025	-0.813805	2.13E-51	-1.69E-46
MS.gene050493	MS.gene29937	0.901333	2.98E-78	-1.69E-46
MS.gene050493	MS.gene31710	0.801051	1.08E-48	-1.69E-46
MS.gene050493	MS.gene34412	0.822979	1.77E-53	-1.69E-46
MS.gene050493	MS.gene38715	-0.802598	5.20E-49	-1.69E-46
MS.gene050493	MS.gene41626	0.803074	4.15E-49	-1.69E-46
MS.gene050493	MS.gene42631	-0.805211	1.49E-49	-1.69E-46
MS.gene050493	MS.gene47889	-0.809529	1.81E-50	-1.69E-46
MS.gene050493	MS.gene51168	-0.80139	9.21E-49	-1.69E-46
MS.gene050493	MS.gene51935	0.801021	1.10E-48	-1.69E-46
MS.gene050493	MS.gene58022	0.805108	1.57E-49	-1.69E-46
MS.gene050493	MS.gene64700	0.80938	1.95E-50	-1.69E-46
MS.gene050493	MS.gene67232	-0.81362	2.33E-51	-1.69E-46
MS.gene050493	MS.gene68631	0.800425	1.45E-48	-1.69E-46
MS.gene050493	MS.gene91801	-0.803912	2.78E-49	-1.69E-46
MS.gene050493	MS.gene94806	-0.801168	1.02E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene050493	MS.gene49471	PPI
MS.gene21833	MS.gene050493	PPI
MS.gene050493	MS.gene024857	PPI
MS.gene043815	MS.gene050493	PPI
MS.gene024858	MS.gene050493	PPI
MS.gene024859	MS.gene050493	PPI
MS.gene024855	MS.gene050493	PPI
MS.gene006418	MS.gene050493	PPI
MS.gene050493	MS.gene071357	PPI
MS.gene050493	MS.gene83474	PPI
MS.gene001037	MS.gene050493	PPI
MS.gene001033	MS.gene050493	PPI
MS.gene050493	MS.gene001034	PPI
MS.gene21827	MS.gene050493	PPI
MS.gene024854	MS.gene050493	PPI
MS.gene050493	MS.gene72809	PPI
MS.gene050493	MS.gene043813	PPI
MS.gene050493	MS.gene21831	PPI
MS.gene006417	MS.gene050493	PPI
MS.gene071361	MS.gene050493	PPI
MS.gene050493	MS.gene82121	PPI
MS.gene49468	MS.gene050493	PPI
MS.gene050493	MS.gene028111	PPI
MS.gene21828	MS.gene050493	PPI
MS.gene050493	MS.gene070944	PPI
MS.gene95802	MS.gene050493	PPI
MS.gene34370	MS.gene050493	PPI
MS.gene050493	MS.gene95801	PPI
MS.gene49472	MS.gene050493	PPI
MS.gene77154	MS.gene050493	PPI
MS.gene050493	MS.gene49470	PPI
MS.gene34371	MS.gene050493	PPI
MS.gene001038	MS.gene050493	PPI
MS.gene050493	MS.gene001032	PPI
MS.gene070945	MS.gene050493	PPI
MS.gene21830	MS.gene050493	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050493.t1	MTR_7g114410	97.159	176	5	0	1	176	1	176	6.61e-127	354
MS.gene050493.t1	MTR_7g114410	96.795	156	5	0	1	156	1	156	1.29e-111	315
MS.gene050493.t1	MTR_3g463610	61.988	171	62	1	4	171	3	173	5.73e-79	234
MS.gene050493.t1	MTR_7g024780	57.955	176	73	1	1	176	1	175	1.80e-70	211
MS.gene050493.t1	MTR_7g024760	59.770	174	68	2	1	174	1	172	1.34e-67	204
MS.gene050493.t1	MTR_7g024765	60.345	174	67	2	1	174	1	172	1.96e-66	201
MS.gene050493.t1	MTR_1g103150	46.784	171	86	2	4	171	19	187	3.59e-48	155
MS.gene050493.t1	MTR_5g006450	43.023	172	95	1	3	171	16	187	2.36e-47	153
MS.gene050493.t1	MTR_1g103110	45.614	171	88	2	4	171	19	187	5.33e-47	152
MS.gene050493.t1	MTR_5g006520	45.029	171	91	1	4	171	1735	1905	9.46e-46	160
MS.gene050493.t1	MTR_5g006470	44.379	169	93	1	4	171	17	185	3.93e-45	147
MS.gene050493.t1	MTR_5g006460	44.000	175	89	5	4	171	18	190	3.17e-42	140
MS.gene050493.t1	MTR_5g006510	43.353	173	90	4	4	171	19	188	3.56e-42	140

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050493.t1	AT3G22560	54.819	166	73	1	8	171	7	172	3.54e-63	193
MS.gene050493.t1	AT2G32030	47.305	167	84	2	8	173	24	187	3.08e-57	178
MS.gene050493.t1	AT2G32020	47.126	174	88	2	1	173	12	182	4.49e-56	175

Find 42 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTGTTGCTTCTAACTATT+GGG	0.152318	7.2:-2984502	MS.gene050493:CDS
TTCTGATTTGTTAAGGTTGC+AGG	0.201561	7.2:-2984421	MS.gene050493:CDS
ATGCTGTTGCTTCTAACTAT+TGG	0.211993	7.2:-2984503	MS.gene050493:CDS
CAAGGCTTCTCAAAGAGTTT+TGG	0.245031	7.2:-2984379	MS.gene050493:CDS
AGACATCTTTGATGAAAGTT+AGG	0.305845	7.2:+2984634	None:intergenic
CAAAGGTACCATCATGGATT+TGG	0.335385	7.2:-2984307	MS.gene050493:CDS
AATAGATCATAAAGAGTAAC+AGG	0.344936	7.2:+2984230	None:intergenic
CGTTGACGATTTCATGTTAT+GGG	0.361603	7.2:-2984718	MS.gene050493:CDS
TTCTAAGAACACCCTCTCTA+AGG	0.383141	7.2:+2984343	None:intergenic
ACGTTGACGATTTCATGTTA+TGG	0.384226	7.2:-2984719	MS.gene050493:CDS
TCAACGTCTGTTAGCTTGAA+TGG	0.418956	7.2:+2984735	None:intergenic
GTTTCTGTGTATCCTTGGTC+AGG	0.429314	7.2:-2984555	MS.gene050493:CDS
GACGATTTCATGTTATGGGC+AGG	0.454598	7.2:-2984714	MS.gene050493:CDS
GACATCTTTGATGAAAGTTA+GGG	0.457791	7.2:+2984635	None:intergenic
CAGGAGACAAAATAATCAAC+AGG	0.459432	7.2:+2984249	None:intergenic
TGGCAAAGGTGGATCTATCT+AGG	0.472926	7.2:-2984770	MS.gene050493:CDS
CGATTTCATGTTATGGGCAG+GGG	0.502678	7.2:-2984712	MS.gene050493:CDS
GCTTCTAACTATTGGGGTCA+AGG	0.506483	7.2:-2984495	MS.gene050493:CDS
TGTCTCAACGACCGTTCCAT+CGG	0.512882	7.2:-2984582	MS.gene050493:CDS
ATCGAATGACGCCAAGGGTG+AGG	0.527528	7.2:+2984606	None:intergenic
GCTGTTGCTTCTAACTATTG+GGG	0.550437	7.2:-2984501	MS.gene050493:CDS
AGAAAGTATACTTATATCAA+AGG	0.562044	7.2:-2984324	MS.gene050493:CDS
CTTCTAACTATTGGGGTCAA+GGG	0.563961	7.2:-2984494	MS.gene050493:CDS
ATCCTTGGTCAGGTGATGAT+AGG	0.566062	7.2:-2984545	MS.gene050493:CDS
GATGGAAAACTTGCAATTCG+AGG	0.572925	7.2:-2984665	MS.gene050493:CDS
TATGACCAACATGGCAAAGG+TGG	0.575392	7.2:-2984781	None:intergenic
ACGATTTCATGTTATGGGCA+GGG	0.580454	7.2:-2984713	MS.gene050493:CDS
GATAGGTGCAAAGCTGACAT+AGG	0.586688	7.2:-2984528	MS.gene050493:CDS
ATGGAAAACTTGCAATTCGA+GGG	0.591968	7.2:-2984664	MS.gene050493:CDS
ATGTCTGCATACCTCACCCT+TGG	0.593939	7.2:-2984617	MS.gene050493:CDS
AGACAGATCGAATGACGCCA+AGG	0.595493	7.2:+2984600	None:intergenic
TTATATCAAAGGTACCATCA+TGG	0.596125	7.2:-2984313	MS.gene050493:CDS
TCATATGACCAACATGGCAA+AGG	0.606489	7.2:-2984784	None:intergenic
AAAGGCTGGGTTCCTTAGAG+AGG	0.612674	7.2:-2984355	MS.gene050493:CDS
ATACACAGAAACAAAACCGA+TGG	0.615405	7.2:+2984566	None:intergenic
AAAGACAGCCAAATCCATGA+TGG	0.623533	7.2:+2984299	None:intergenic
CAGAAACAAAACCGATGGAA+CGG	0.641063	7.2:+2984571	None:intergenic
GACAGATCGAATGACGCCAA+GGG	0.643672	7.2:+2984601	None:intergenic
AAGGCTGGGTTCCTTAGAGA+GGG	0.646930	7.2:-2984354	MS.gene050493:CDS
GAGACAAAATAATCAACAGG+AGG	0.670315	7.2:+2984252	None:intergenic
CACCTATCATCACCTGACCA+AGG	0.677858	7.2:+2984543	None:intergenic
ATCTATCTAGGATATCTCTG+AGG	0.735761	7.2:-2984758	MS.gene050493:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGAAAGTATACTTATATCAA+AGG	-	chr7.2:2984683-2984702	MS.gene050493:CDS	20.0%
!!!	ATGATTTTTCTGATTTGTTA+AGG	-	chr7.2:2984579-2984598	MS.gene050493:CDS	20.0%
	AGACATCTTTGATGAAAGTT+AGG	+	chr7.2:2984376-2984395	None:intergenic	30.0%
	GACATCTTTGATGAAAGTTA+GGG	+	chr7.2:2984375-2984394	None:intergenic	30.0%
!	TTTTACTTACGTGGATAACA+AGG	-	chr7.2:2984610-2984629	MS.gene050493:CDS	30.0%
!!	TTATATCAAAGGTACCATCA+TGG	-	chr7.2:2984694-2984713	MS.gene050493:CDS	30.0%
!!!	AGCAATTTTGATTGCTTTTG+TGG	+	chr7.2:2984543-2984562	None:intergenic	30.0%
!!!	TTCTCAAAGAGTTTTGGAAA+AGG	-	chr7.2:2984634-2984653	MS.gene050493:CDS	30.0%
	AAGTAACCAAAAACATCCGA+TGG	-	chr7.2:2984324-2984343	MS.gene050493:CDS	35.0%
	ACGTTGACGATTTCATGTTA+TGG	-	chr7.2:2984288-2984307	MS.gene050493:CDS	35.0%
	ATACACAGAAACAAAACCGA+TGG	+	chr7.2:2984444-2984463	None:intergenic	35.0%
	ATGGAAAACTTGCAATTCGA+GGG	-	chr7.2:2984343-2984362	MS.gene050493:CDS	35.0%
	CAGGAGACAAAATAATCAAC+AGG	+	chr7.2:2984761-2984780	None:intergenic	35.0%
	CGTTGACGATTTCATGTTAT+GGG	-	chr7.2:2984289-2984308	MS.gene050493:CDS	35.0%
	GAGACAAAATAATCAACAGG+AGG	+	chr7.2:2984758-2984777	None:intergenic	35.0%
!	AGTTTTCCATCGGATGTTTT+TGG	+	chr7.2:2984333-2984352	None:intergenic	35.0%
!	ATCTATCTAGGATATCTCTG+AGG	-	chr7.2:2984249-2984268	MS.gene050493:CDS	35.0%
!	ATGCTGTTGCTTCTAACTAT+TGG	-	chr7.2:2984504-2984523	MS.gene050493:CDS	35.0%
!	TCGAATTGCAAGTTTTCCAT+CGG	+	chr7.2:2984343-2984362	None:intergenic	35.0%
!	TGCTGTTGCTTCTAACTATT+GGG	-	chr7.2:2984505-2984524	MS.gene050493:CDS	35.0%
!!	TTCTGATTTGTTAAGGTTGC+AGG	-	chr7.2:2984586-2984605	MS.gene050493:CDS	35.0%
!!!	AAAGAGTTTTGGAAAAGGCT+GGG	-	chr7.2:2984639-2984658	MS.gene050493:CDS	35.0%
!!!	GTTTTGTTTCTGTGTATCCT+TGG	-	chr7.2:2984447-2984466	MS.gene050493:CDS	35.0%
	AAAGACAGCCAAATCCATGA+TGG	+	chr7.2:2984711-2984730	None:intergenic	40.0%
	ACGATTTCATGTTATGGGCA+GGG	-	chr7.2:2984294-2984313	MS.gene050493:CDS	40.0%
	CAGAAACAAAACCGATGGAA+CGG	+	chr7.2:2984439-2984458	None:intergenic	40.0%
	CTTCTAACTATTGGGGTCAA+GGG	-	chr7.2:2984513-2984532	MS.gene050493:CDS	40.0%
	GATGGAAAACTTGCAATTCG+AGG	-	chr7.2:2984342-2984361	MS.gene050493:CDS	40.0%
!	CAAGGCTTCTCAAAGAGTTT+TGG	-	chr7.2:2984628-2984647	MS.gene050493:CDS	40.0%
!	GCTGTTGCTTCTAACTATTG+GGG	-	chr7.2:2984506-2984525	MS.gene050493:CDS	40.0%
!	TCAACGTCTGTTAGCTTGAA+TGG	+	chr7.2:2984275-2984294	None:intergenic	40.0%
!	TTCTAAGAACACCCTCTCTA+AGG	+	chr7.2:2984667-2984686	None:intergenic	40.0%
!!	CAAAGGTACCATCATGGATT+TGG	-	chr7.2:2984700-2984719	MS.gene050493:CDS	40.0%
!!!	CAAAGAGTTTTGGAAAAGGC+TGG	-	chr7.2:2984638-2984657	MS.gene050493:CDS	40.0%
	ATCCTTGGTCAGGTGATGAT+AGG	-	chr7.2:2984462-2984481	MS.gene050493:CDS	45.0%
	CGATTTCATGTTATGGGCAG+GGG	-	chr7.2:2984295-2984314	MS.gene050493:CDS	45.0%
	GACGATTTCATGTTATGGGC+AGG	-	chr7.2:2984293-2984312	MS.gene050493:CDS	45.0%
	GATAGGTGCAAAGCTGACAT+AGG	-	chr7.2:2984479-2984498	MS.gene050493:CDS	45.0%
	GCTTCTAACTATTGGGGTCA+AGG	-	chr7.2:2984512-2984531	MS.gene050493:CDS	45.0%
	GTTTCTGTGTATCCTTGGTC+AGG	-	chr7.2:2984452-2984471	MS.gene050493:CDS	45.0%
	TGGCAAAGGTGGATCTATCT+AGG	-	chr7.2:2984237-2984256	MS.gene050493:CDS	45.0%
!	GTTGCAGGCTTTTACTTACG+TGG	-	chr7.2:2984601-2984620	MS.gene050493:CDS	45.0%
	AAAGGCTGGGTTCCTTAGAG+AGG	-	chr7.2:2984652-2984671	MS.gene050493:CDS	50.0%
	AAGGCTGGGTTCCTTAGAGA+GGG	-	chr7.2:2984653-2984672	MS.gene050493:CDS	50.0%
	AGACAGATCGAATGACGCCA+AGG	+	chr7.2:2984410-2984429	None:intergenic	50.0%
	ATGTCTGCATACCTCACCCT+TGG	-	chr7.2:2984390-2984409	MS.gene050493:CDS	50.0%
	CACCTATCATCACCTGACCA+AGG	+	chr7.2:2984467-2984486	None:intergenic	50.0%
	GACAGATCGAATGACGCCAA+GGG	+	chr7.2:2984409-2984428	None:intergenic	50.0%
	TGTCTCAACGACCGTTCCAT+CGG	-	chr7.2:2984425-2984444	MS.gene050493:CDS	50.0%
	ATCGAATGACGCCAAGGGTG+AGG	+	chr7.2:2984404-2984423	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	2984236	2984793	2984236	ID=MS.gene050493
chr7.2	mRNA	2984236	2984793	2984236	ID=MS.gene050493.t1;Parent=MS.gene050493
chr7.2	exon	2984236	2984793	2984236	ID=MS.gene050493.t1.exon1;Parent=MS.gene050493.t1
chr7.2	CDS	2984236	2984793	2984236	ID=cds.MS.gene050493.t1;Parent=MS.gene050493.t1

Gene Sequence

>MS.gene050493

ATGGCAAAGGTGGATCTATCTAGGATATCTCTGAGGCCATTCAAGCTAACAGACGTTGACGATTTCATGTTATGGGCAGGGGACGATGAAGTAACCAAAAACATCCGATGGAAAACTTGCAATTCGAGGGAAGAAGCCCTAACTTTCATCAAAGATGTCTGCATACCTCACCCTTGGCGTCATTCGATCTGTCTCAACGACCGTTCCATCGGTTTTGTTTCTGTGTATCCTTGGTCAGGTGATGATAGGTGCAAAGCTGACATAGGTTATGCTGTTGCTTCTAACTATTGGGGTCAAGGGATAGCCACAAAAGCAATCAAAATTGCTCTGTCTCAAGTGTTTAATGATTTTTCTGATTTGTTAAGGTTGCAGGCTTTTACTTACGTGGATAACAAGGCTTCTCAAAGAGTTTTGGAAAAGGCTGGGTTCCTTAGAGAGGGTGTTCTTAGAAAGTATACTTATATCAAAGGTACCATCATGGATTTGGCTGTCTTTAGTTTTTTGTCAACAGATGAAATTCCTCCTGTTGATTATTTTGTCTCCTGTTACTCTTTATGA

Protein sequence

>MS.gene050493.t1

MAKVDLSRISLRPFKLTDVDDFMLWAGDDEVTKNIRWKTCNSREEALTFIKDVCIPHPWRHSICLNDRSIGFVSVYPWSGDDRCKADIGYAVASNYWGQGIATKAIKIALSQVFNDFSDLLRLQAFTYVDNKASQRVLEKAGFLREGVLRKYTYIKGTIMDLAVFSFLSTDEIPPVDYFVSCYSL