AlfalfaGEDB | MS.gene22888

M. sativa cultivar XinJiangDaYe / MS.gene22888

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22888.t1	XP_003626377.2	97.7	176	4	0	1	176	1	176	1.00E-95	359.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22888.t1	O34569	29.7	175	115	4	4	172	5	177	1.1e-13	78.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22888.t1	G7L0S4	97.7	176	4	0	1	176	1	176	7.3e-96	359.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene22888.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene22888	-0.808007	3.83E-50	-1.69E-46
MS.gene049281	MS.gene22888	0.837175	6.07E-57	-1.69E-46
MS.gene049285	MS.gene22888	-0.805988	1.02E-49	-1.69E-46
MS.gene049320	MS.gene22888	-0.833556	4.98E-56	-1.69E-46
MS.gene049483	MS.gene22888	0.802051	6.74E-49	-1.69E-46
MS.gene050163	MS.gene22888	-0.812712	3.69E-51	-1.69E-46
MS.gene050165	MS.gene22888	-0.814137	1.80E-51	-1.69E-46
MS.gene050493	MS.gene22888	0.905783	2.98E-80	-1.69E-46
MS.gene050504	MS.gene22888	-0.830678	2.57E-55	-1.69E-46
MS.gene050612	MS.gene22888	-0.81839	2.01E-52	-1.69E-46
MS.gene050699	MS.gene22888	-0.804857	1.77E-49	-1.69E-46
MS.gene050883	MS.gene22888	0.822651	2.11E-53	-1.69E-46
MS.gene050986	MS.gene22888	-0.805545	1.27E-49	-1.69E-46
MS.gene051127	MS.gene22888	0.8299	3.97E-55	-1.69E-46
MS.gene053420	MS.gene22888	0.812952	3.27E-51	-1.69E-46
MS.gene053561	MS.gene22888	0.824852	6.45E-54	-1.69E-46
MS.gene05470	MS.gene22888	-0.806094	9.72E-50	-1.69E-46
MS.gene054954	MS.gene22888	-0.814379	1.59E-51	-1.69E-46
MS.gene054959	MS.gene22888	0.820558	6.44E-53	-1.69E-46
MS.gene055154	MS.gene22888	0.817516	3.17E-52	-1.69E-46
MS.gene055362	MS.gene22888	-0.832063	1.17E-55	-1.69E-46
MS.gene056504	MS.gene22888	-0.802513	5.41E-49	-1.69E-46
MS.gene056657	MS.gene22888	-0.802842	4.63E-49	-1.69E-46
MS.gene056761	MS.gene22888	0.856533	3.02E-62	-1.69E-46
MS.gene058268	MS.gene22888	0.820144	8.01E-53	-1.69E-46
MS.gene058269	MS.gene22888	0.808897	2.47E-50	-1.69E-46
MS.gene058419	MS.gene22888	-0.815286	9.99E-52	-1.69E-46
MS.gene059022	MS.gene22888	-0.858239	9.44E-63	-1.69E-46
MS.gene059677	MS.gene22888	-0.834129	3.58E-56	-1.69E-46
MS.gene059865	MS.gene22888	-0.802011	6.87E-49	-1.69E-46
MS.gene060337	MS.gene22888	0.846413	2.21E-59	-1.69E-46
MS.gene060703	MS.gene22888	-0.812474	4.16E-51	-1.69E-46
MS.gene06077	MS.gene22888	-0.826516	2.60E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34371	MS.gene22888	PPI
MS.gene001038	MS.gene22888	PPI
MS.gene070945	MS.gene22888	PPI
MS.gene21830	MS.gene22888	PPI
MS.gene21833	MS.gene22888	PPI
MS.gene043815	MS.gene22888	PPI
MS.gene001034	MS.gene22888	PPI
MS.gene95802	MS.gene22888	PPI
MS.gene95801	MS.gene22888	PPI
MS.gene024858	MS.gene22888	PPI
MS.gene024859	MS.gene22888	PPI
MS.gene024855	MS.gene22888	PPI
MS.gene006418	MS.gene22888	PPI
MS.gene83474	MS.gene22888	PPI
MS.gene49470	MS.gene22888	PPI
MS.gene82121	MS.gene22888	PPI
MS.gene21831	MS.gene22888	PPI
MS.gene028111	MS.gene22888	PPI
MS.gene001037	MS.gene22888	PPI
MS.gene22888	MS.gene024857	PPI
MS.gene070944	MS.gene22888	PPI
MS.gene21827	MS.gene22888	PPI
MS.gene024854	MS.gene22888	PPI
MS.gene22888	MS.gene071357	PPI
MS.gene006417	MS.gene22888	PPI
MS.gene49471	MS.gene22888	PPI
MS.gene001033	MS.gene22888	PPI
MS.gene071361	MS.gene22888	PPI
MS.gene72809	MS.gene22888	PPI
MS.gene49468	MS.gene22888	PPI
MS.gene21828	MS.gene22888	PPI
MS.gene34370	MS.gene22888	PPI
MS.gene49472	MS.gene22888	PPI
MS.gene77154	MS.gene22888	PPI
MS.gene001032	MS.gene22888	PPI
MS.gene043813	MS.gene22888	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22888.t1	MTR_7g114410	97.727	176	4	0	1	176	1	176	2.02e-127	355
MS.gene22888.t1	MTR_7g114410	97.436	156	4	0	1	156	1	156	4.11e-112	316
MS.gene22888.t1	MTR_3g463610	62.573	171	61	1	4	171	3	173	1.21e-79	236
MS.gene22888.t1	MTR_7g024780	58.523	176	72	1	1	176	1	175	4.06e-71	213
MS.gene22888.t1	MTR_7g024765	60.920	174	66	2	1	174	1	172	4.52e-67	203
MS.gene22888.t1	MTR_7g024760	59.195	174	69	2	1	174	1	172	7.32e-67	202
MS.gene22888.t1	MTR_1g103150	46.784	171	86	2	4	171	19	187	6.47e-48	154
MS.gene22888.t1	MTR_1g103110	45.614	171	88	2	4	171	19	187	7.08e-47	152
MS.gene22888.t1	MTR_5g006450	42.442	172	96	1	3	171	16	187	1.47e-46	151
MS.gene22888.t1	MTR_5g006520	44.444	171	92	1	4	171	1735	1905	4.15e-45	159
MS.gene22888.t1	MTR_5g006470	43.787	169	94	1	4	171	17	185	2.12e-44	145
MS.gene22888.t1	MTR_5g006510	42.775	173	91	4	4	171	19	188	1.83e-41	138
MS.gene22888.t1	MTR_5g006460	43.429	175	90	5	4	171	18	190	1.86e-41	138

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22888.t1	AT3G22560	55.422	166	72	1	8	171	7	172	9.75e-64	194
MS.gene22888.t1	AT2G32030	47.305	167	84	2	8	173	24	187	4.77e-57	178
MS.gene22888.t1	AT2G32020	47.701	174	87	2	1	173	12	182	1.17e-56	177

Find 0 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGAAAGTATACTTATATCAA+AGG	+	51741:26982-27001	MS.gene22888:CDS	20.0%
!!!	ATGATTTTTCTGATTTGTTA+AGG	+	51741:26878-26897	MS.gene22888:CDS	20.0%
	AGACATCTTTGATGAAAGTT+AGG	-	51741:26675-26694	MS.gene22888:intergenic	30.0%
	GACATCTTTGATGAAAGTTA+GGG	-	51741:26674-26693	MS.gene22888:intergenic	30.0%
!	TTTTACTTACGTGGATAACA+AGG	+	51741:26909-26928	MS.gene22888:CDS	30.0%
!!	TTATATCAAAGGTACCATCA+TGG	+	51741:26993-27012	MS.gene22888:CDS	30.0%
!!!	AGCAATTTTGATTGCTTTTG+TGG	-	51741:26842-26861	MS.gene22888:intergenic	30.0%
!!!	TTCTCAAAGAGTTTTGGAAA+AGG	+	51741:26933-26952	MS.gene22888:CDS	30.0%
	AAGTAACCAAAAACATCCGA+TGG	+	51741:26623-26642	MS.gene22888:CDS	35.0%
	ACGTTGACGATTTCATGTTA+TGG	+	51741:26587-26606	MS.gene22888:CDS	35.0%
	ATACACAGAAACAAAACCGA+TGG	-	51741:26743-26762	MS.gene22888:intergenic	35.0%
	ATGGAAAACTTGCAATTCGA+GGG	+	51741:26642-26661	MS.gene22888:CDS	35.0%
	CAGGAGACAAAATAATCAAC+AGG	-	51741:27060-27079	MS.gene22888:intergenic	35.0%
	CGTTGACGATTTCATGTTAT+GGG	+	51741:26588-26607	MS.gene22888:CDS	35.0%
	GAGACAAAATAATCAACAGG+AGG	-	51741:27057-27076	MS.gene22888:intergenic	35.0%
!	AGTTTTCCATCGGATGTTTT+TGG	-	51741:26632-26651	MS.gene22888:intergenic	35.0%
!	ATCTATCTAGGATATCTCTG+AGG	+	51741:26548-26567	MS.gene22888:CDS	35.0%
!	ATGCTGTTGCTTCTAACTAT+TGG	+	51741:26803-26822	MS.gene22888:CDS	35.0%
!	TCGAATTGCAAGTTTTCCAT+CGG	-	51741:26642-26661	MS.gene22888:intergenic	35.0%
!	TGCTGTTGCTTCTAACTATT+GGG	+	51741:26804-26823	MS.gene22888:CDS	35.0%
!!	TTCTGATTTGTTAAGGTTGC+AGG	+	51741:26885-26904	MS.gene22888:CDS	35.0%
!!!	AAAGAGTTTTGGAAAAGGCT+GGG	+	51741:26938-26957	MS.gene22888:CDS	35.0%
!!!	GTTTTGTTTCTGTGTATCCT+TGG	+	51741:26746-26765	MS.gene22888:CDS	35.0%
	AAAGACAGCCAAATCCATGA+TGG	-	51741:27010-27029	MS.gene22887:intergenic	40.0%
	ACGATTTCATGTTATGGGCA+GGG	+	51741:26593-26612	MS.gene22888:CDS	40.0%
	CAGAAACAAAACCGATGGAA+CGG	-	51741:26738-26757	MS.gene22888:intergenic	40.0%
	CTTCTAACTATTGGGGTCAA+GGG	+	51741:26812-26831	MS.gene22888:CDS	40.0%
	GATGGAAAACTTGCAATTCG+AGG	+	51741:26641-26660	MS.gene22888:CDS	40.0%
!	CAAGGCTTCTCAAAGAGTTT+TGG	+	51741:26927-26946	MS.gene22888:CDS	40.0%
!	GCTGTTGCTTCTAACTATTG+GGG	+	51741:26805-26824	MS.gene22888:CDS	40.0%
!	TCAACGTCTGTTAGCTTGAA+TGG	-	51741:26574-26593	MS.gene22888:intergenic	40.0%
!	TTCTAAGAACACCCTCTCTA+AGG	-	51741:26966-26985	MS.gene22888:intergenic	40.0%
!!	CAAAGGTACCATCATGGATT+TGG	+	51741:26999-27018	MS.gene22888:CDS	40.0%
!!!	CAAAGAGTTTTGGAAAAGGC+TGG	+	51741:26937-26956	MS.gene22888:CDS	40.0%
	ATCCTTGGTCAGGTGATGAT+AGG	+	51741:26761-26780	MS.gene22888:CDS	45.0%
	CGATTTCATGTTATGGGCAG+GGG	+	51741:26594-26613	MS.gene22888:CDS	45.0%
	GACGATTTCATGTTATGGGC+AGG	+	51741:26592-26611	MS.gene22888:CDS	45.0%
	GATAGGTGCAAAGCTGACAT+AGG	+	51741:26778-26797	MS.gene22888:CDS	45.0%
	GCTTCTAACTATTGGGGTCA+AGG	+	51741:26811-26830	MS.gene22888:CDS	45.0%
	GTTTCTGTGTATCCTTGGTC+AGG	+	51741:26751-26770	MS.gene22888:CDS	45.0%
	TGGCAAAGGTGGATCTATCT+AGG	+	51741:26536-26555	MS.gene22888:CDS	45.0%
!	GTTGCAGGCTTTTACTTACG+TGG	+	51741:26900-26919	MS.gene22888:CDS	45.0%
	AAAGGCTGGGTTCCTTAGAG+AGG	+	51741:26951-26970	MS.gene22888:CDS	50.0%
	AAGGCTGGGTTCCTTAGAGA+GGG	+	51741:26952-26971	MS.gene22888:CDS	50.0%
	AGACAGATCGAATGACGCCA+AGG	-	51741:26709-26728	MS.gene22888:intergenic	50.0%
	ATGTCTGCATACCTCACCCT+TGG	+	51741:26689-26708	MS.gene22888:CDS	50.0%
	CACCTATCATCACCTGACCA+AGG	-	51741:26766-26785	MS.gene22888:intergenic	50.0%
	GACAGATCGAATGACGCCAA+GGG	-	51741:26708-26727	MS.gene22888:intergenic	50.0%
	ATCGAATGACGCCAAGGGTG+AGG	-	51741:26703-26722	MS.gene22888:intergenic	55.0%
	TGTCTCGACGACCGTTCCAT+CGG	+	51741:26724-26743	MS.gene22888:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
51741	gene	26535	27092	26535	ID=MS.gene22888
51741	mRNA	26535	27092	26535	ID=MS.gene22888.t1;Parent=MS.gene22888
51741	exon	26535	27092	26535	ID=MS.gene22888.t1.exon1;Parent=MS.gene22888.t1
51741	CDS	26535	27092	26535	ID=cds.MS.gene22888.t1;Parent=MS.gene22888.t1

Gene Sequence

>MS.gene22888

ATGGCAAAGGTGGATCTATCTAGGATATCTCTGAGGCCATTCAAGCTAACAGACGTTGACGATTTCATGTTATGGGCAGGGGACGATGAAGTAACCAAAAACATCCGATGGAAAACTTGCAATTCGAGGGAAGAAGCCCTAACTTTCATCAAAGATGTCTGCATACCTCACCCTTGGCGTCATTCGATCTGTCTCGACGACCGTTCCATCGGTTTTGTTTCTGTGTATCCTTGGTCAGGTGATGATAGGTGCAAAGCTGACATAGGTTATGCTGTTGCTTCTAACTATTGGGGTCAAGGGATAGCCACAAAAGCAATCAAAATTGCTCTGTCTCAAGTGTTTAATGATTTTTCTGATTTGTTAAGGTTGCAGGCTTTTACTTACGTGGATAACAAGGCTTCTCAAAGAGTTTTGGAAAAGGCTGGGTTCCTTAGAGAGGGTGTTCTTAGAAAGTATACTTATATCAAAGGTACCATCATGGATTTGGCTGTCTTTAGTTTTTTGTCAACAGATGAAATTCCTCCTGTTGATTATTTTGTCTCCTGTTACTCTTTATGA

Protein sequence

>MS.gene22888.t1

MAKVDLSRISLRPFKLTDVDDFMLWAGDDEVTKNIRWKTCNSREEALTFIKDVCIPHPWRHSICLDDRSIGFVSVYPWSGDDRCKADIGYAVASNYWGQGIATKAIKIALSQVFNDFSDLLRLQAFTYVDNKASQRVLEKAGFLREGVLRKYTYIKGTIMDLAVFSFLSTDEIPPVDYFVSCYSL