AlfalfaGEDB | MS.gene74082

M. sativa cultivar XinJiangDaYe / MS.gene74082

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74082.t1	XP_003621899.2	94.9	176	9	0	1	176	1	176	1.30E-84	322.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74082.t1	P05332	27.3	172	117	3	5	169	5	175	1.1e-10	68.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74082.t1	G7KXV2	94.9	176	9	0	1	176	1	176	9.4e-85	322.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene74082.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049293	MS.gene74082	-0.824497	7.82E-54	-1.69E-46
MS.gene049378	MS.gene74082	0.807527	4.84E-50	-1.69E-46
MS.gene049669	MS.gene74082	0.81496	1.18E-51	-1.69E-46
MS.gene052176	MS.gene74082	0.821118	4.79E-53	-1.69E-46
MS.gene052376	MS.gene74082	0.804558	2.04E-49	-1.69E-46
MS.gene053340	MS.gene74082	0.803515	3.36E-49	-1.69E-46
MS.gene054946	MS.gene74082	-0.804303	2.30E-49	-1.69E-46
MS.gene059710	MS.gene74082	0.803181	3.94E-49	-1.69E-46
MS.gene060879	MS.gene74082	0.835157	1.98E-56	-1.69E-46
MS.gene061040	MS.gene74082	-0.802681	5.00E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene21827	MS.gene74082	PPI
MS.gene74082	MS.gene001032	PPI
MS.gene74082	MS.gene49471	PPI
MS.gene74082	MS.gene024857	PPI
MS.gene024854	MS.gene74082	PPI
MS.gene028111	MS.gene74082	PPI
MS.gene001037	MS.gene74082	PPI
MS.gene74082	MS.gene071357	PPI
MS.gene006417	MS.gene74082	PPI
MS.gene001033	MS.gene74082	PPI
MS.gene071361	MS.gene74082	PPI
MS.gene49468	MS.gene74082	PPI
MS.gene77154	MS.gene74082	PPI
MS.gene043813	MS.gene74082	PPI
MS.gene34371	MS.gene74082	PPI
MS.gene070945	MS.gene74082	PPI
MS.gene74082	MS.gene72809	PPI
MS.gene21833	MS.gene74082	PPI
MS.gene043815	MS.gene74082	PPI
MS.gene21828	MS.gene74082	PPI
MS.gene34370	MS.gene74082	PPI
MS.gene49472	MS.gene74082	PPI
MS.gene024859	MS.gene74082	PPI
MS.gene024855	MS.gene74082	PPI
MS.gene83474	MS.gene74082	PPI
MS.gene001038	MS.gene74082	PPI
MS.gene74082	MS.gene070944	PPI
MS.gene21830	MS.gene74082	PPI
MS.gene82121	MS.gene74082	PPI
MS.gene001034	MS.gene74082	PPI
MS.gene21831	MS.gene74082	PPI
MS.gene95801	MS.gene74082	PPI
MS.gene95802	MS.gene74082	PPI
MS.gene024858	MS.gene74082	PPI
MS.gene74082	MS.gene49470	PPI
MS.gene006418	MS.gene74082	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74082.t1	MTR_7g024760	95.455	176	8	0	1	176	1	176	5.76e-126	351
MS.gene74082.t1	MTR_7g024765	82.659	173	30	0	1	173	1	173	4.03e-105	299
MS.gene74082.t1	MTR_7g024780	68.786	173	53	1	1	172	1	173	3.79e-84	246
MS.gene74082.t1	MTR_7g114410	60.714	168	64	2	7	172	7	174	3.15e-69	208
MS.gene74082.t1	MTR_7g114410	59.868	152	59	2	7	156	7	158	7.03e-60	184
MS.gene74082.t1	MTR_3g463610	51.190	168	77	2	7	169	6	173	1.35e-53	170
MS.gene74082.t1	MTR_1g103110	45.732	164	86	3	7	167	22	185	6.30e-43	141
MS.gene74082.t1	MTR_5g006470	43.373	166	91	2	7	169	20	185	2.89e-39	132
MS.gene74082.t1	MTR_5g006460	42.690	171	89	6	7	169	21	190	1.25e-38	130
MS.gene74082.t1	MTR_1g103150	42.771	166	92	3	7	169	22	187	1.79e-38	130
MS.gene74082.t1	MTR_5g006510	42.262	168	91	4	7	169	22	188	6.75e-38	129
MS.gene74082.t1	MTR_5g006450	42.262	168	92	3	7	169	20	187	6.78e-38	129
MS.gene74082.t1	MTR_5g006520	40.476	168	95	3	7	169	1738	1905	4.48e-32	121

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74082.t1	AT2G32020	41.618	173	97	3	1	171	12	182	2.88e-45	147
MS.gene74082.t1	AT3G22560	45.783	166	86	3	8	169	7	172	4.78e-44	144
MS.gene74082.t1	AT2G32030	40.361	166	95	3	8	171	24	187	4.96e-42	139

Find 49 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTATTGGGTTCAACTATT+GGG	0.213071	7.4:+76628701	MS.gene74082:CDS
GCAAAGTTTAAAGCAGATTT+AGG	0.222691	7.4:+76628675	MS.gene74082:CDS
ATGCTATTGGGTTCAACTAT+TGG	0.239859	7.4:+76628700	MS.gene74082:CDS
TGTCTTAATGATCACTCTAT+TGG	0.260890	7.4:+76628624	MS.gene74082:CDS
TCCTGATTTGGTAAGGTTGC+AGG	0.261225	7.4:+76628782	MS.gene74082:CDS
GCAGATTTAGGCTATGCTAT+TGG	0.284212	7.4:+76628687	MS.gene74082:CDS
ATTCCAAGACTTTCCTGATT+TGG	0.305046	7.4:+76628770	MS.gene74082:CDS
CAGATTTAGGCTATGCTATT+GGG	0.336174	7.4:+76628688	MS.gene74082:CDS
TTCTGAACAATCCCTCCCTT+TGG	0.370350	7.4:-76628860	None:intergenic
CAAAAGAATCTTCACTGCTT+TGG	0.379382	7.4:-76628739	None:intergenic
TTAAACTTTGCATCAGGATA+AGG	0.382959	7.4:-76628663	None:intergenic
GCCTGCAACCTTACCAAATC+AGG	0.389988	7.4:-76628783	None:intergenic
TCAGCATCACTTTCTTTGAA+TGG	0.406038	7.4:-76628474	None:intergenic
GAAAGGGAATTTATAACAAA+GGG	0.412673	7.4:+76628880	MS.gene74082:CDS
TCTGCTTTAAACTTTGCATC+AGG	0.422143	7.4:-76628669	None:intergenic
CATTAAGACAAATTGATAGT+TGG	0.432253	7.4:-76628611	None:intergenic
CAAAGACTGTTGGAAAAGGT+TGG	0.435336	7.4:+76628834	MS.gene74082:CDS
TTCTCAAAGACTGTTGGAAA+AGG	0.441158	7.4:+76628830	MS.gene74082:CDS
CATCACTTTCTTTGAATGGC+CGG	0.455673	7.4:-76628470	None:intergenic
AGACAAATTGATAGTTGGAA+TGG	0.464313	7.4:-76628606	None:intergenic
CTTTGAGAAGCCATGTTCTC+CGG	0.475896	7.4:-76628816	None:intergenic
AAGACTTTCCTGATTTGGTA+AGG	0.487902	7.4:+76628775	MS.gene74082:CDS
CATGGCTTCTCAAAGACTGT+TGG	0.500304	7.4:+76628824	MS.gene74082:CDS
TTACCAAATCAGGAAAGTCT+TGG	0.502664	7.4:-76628773	None:intergenic
CTGCTTTGGTAGCTACACCT+TGG	0.510305	7.4:-76628725	None:intergenic
CGATGAAATTCCTCATGCTA+AGG	0.513932	7.4:+76628941	MS.gene74082:CDS
GGGTTCAACTATTGGGGCCA+AGG	0.514130	7.4:+76628708	MS.gene74082:CDS
TGCTGACGATGTGTTATTGT+GGG	0.528824	7.4:+76628491	MS.gene74082:CDS
GATTGGAGTTATGCAAATCA+AGG	0.538998	7.4:+76628544	MS.gene74082:CDS
AGAAAGGGAATTTATAACAA+AGG	0.541022	7.4:+76628879	MS.gene74082:CDS
GTTGCAGGCTAATACTCGTC+CGG	0.543008	7.4:+76628797	MS.gene74082:CDS
GATGAAATTCCTCATGCTAA+GGG	0.544681	7.4:+76628942	MS.gene74082:CDS
TGGAAAAGGTTGGTTTCCAA+AGG	0.552140	7.4:+76628844	MS.gene74082:CDS
AGGGAGGGATTGTTCAGAAA+GGG	0.555894	7.4:+76628864	MS.gene74082:CDS
TAATACTCGTCCGGAGAACA+TGG	0.558191	7.4:+76628806	MS.gene74082:CDS
AGTCATGCAATATATGCGAT+TGG	0.560410	7.4:+76628527	MS.gene74082:CDS
GGAAAAGGTTGGTTTCCAAA+GGG	0.561564	7.4:+76628845	MS.gene74082:CDS
GCTATTGGGTTCAACTATTG+GGG	0.562007	7.4:+76628702	MS.gene74082:CDS
AAAGGTTGGTTTCCAAAGGG+AGG	0.574627	7.4:+76628848	MS.gene74082:CDS
ATGAATCAAGGATCACTCTC+CGG	0.579292	7.4:+76628451	MS.gene74082:CDS
TGGGAATAGATCATGAATCA+AGG	0.581097	7.4:+76628439	MS.gene74082:CDS
AAGGGAGGGATTGTTCAGAA+AGG	0.588499	7.4:+76628863	MS.gene74082:CDS
AAGGTTGGTTTCCAAAGGGA+GGG	0.591625	7.4:+76628849	MS.gene74082:CDS
ATGCTGACGATGTGTTATTG+TGG	0.596185	7.4:+76628490	MS.gene74082:CDS
ACTAATTAACCCTTAGCATG+AGG	0.612515	7.4:-76628951	None:intergenic
ATTGGAGTTATGCAAATCAA+GGG	0.627871	7.4:+76628545	MS.gene74082:CDS
TTATAACAAAGGGAATGTAG+AGG	0.632962	7.4:+76628890	MS.gene74082:CDS
CTTAGCATGAGGAATTTCAT+CGG	0.640114	7.4:-76628940	None:intergenic
ACTTTGCATCAGGATAAGGA+AGG	0.674910	7.4:-76628659	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAAGGGAATTTATAACAA+AGG	+	chr7.4:76628879-76628898	MS.gene74082:CDS	25.0%
!	GAAAGGGAATTTATAACAAA+GGG	+	chr7.4:76628880-76628899	MS.gene74082:CDS	25.0%
!!	CATTAAGACAAATTGATAGT+TGG	-	chr7.4:76628614-76628633	None:intergenic	25.0%
	ATTGGAGTTATGCAAATCAA+GGG	+	chr7.4:76628545-76628564	MS.gene74082:CDS	30.0%
	TGTCTTAATGATCACTCTAT+TGG	+	chr7.4:76628624-76628643	MS.gene74082:CDS	30.0%
	TTAAACTTTGCATCAGGATA+AGG	-	chr7.4:76628666-76628685	None:intergenic	30.0%
	TTATAACAAAGGGAATGTAG+AGG	+	chr7.4:76628890-76628909	MS.gene74082:CDS	30.0%
!	AGACAAATTGATAGTTGGAA+TGG	-	chr7.4:76628609-76628628	None:intergenic	30.0%
!	GCAAAGTTTAAAGCAGATTT+AGG	+	chr7.4:76628675-76628694	MS.gene74082:CDS	30.0%
	AGTCATGCAATATATGCGAT+TGG	+	chr7.4:76628527-76628546	MS.gene74082:CDS	35.0%
	ATGCTATTGGGTTCAACTAT+TGG	+	chr7.4:76628700-76628719	MS.gene74082:CDS	35.0%
	ATTCCAAGACTTTCCTGATT+TGG	+	chr7.4:76628770-76628789	MS.gene74082:CDS	35.0%
	CAAAAGAATCTTCACTGCTT+TGG	-	chr7.4:76628742-76628761	None:intergenic	35.0%
	CAGATTTAGGCTATGCTATT+GGG	+	chr7.4:76628688-76628707	MS.gene74082:CDS	35.0%
	CTTAGCATGAGGAATTTCAT+CGG	-	chr7.4:76628943-76628962	None:intergenic	35.0%
	GATGAAATTCCTCATGCTAA+GGG	+	chr7.4:76628942-76628961	MS.gene74082:CDS	35.0%
	GATTGGAGTTATGCAAATCA+AGG	+	chr7.4:76628544-76628563	MS.gene74082:CDS	35.0%
	TCAGCATCACTTTCTTTGAA+TGG	-	chr7.4:76628477-76628496	None:intergenic	35.0%
	TGCTATTGGGTTCAACTATT+GGG	+	chr7.4:76628701-76628720	MS.gene74082:CDS	35.0%
	TGGGAATAGATCATGAATCA+AGG	+	chr7.4:76628439-76628458	MS.gene74082:CDS	35.0%
	TTACCAAATCAGGAAAGTCT+TGG	-	chr7.4:76628776-76628795	None:intergenic	35.0%
	TTCTCAAAGACTGTTGGAAA+AGG	+	chr7.4:76628830-76628849	MS.gene74082:CDS	35.0%
!	TCTGCTTTAAACTTTGCATC+AGG	-	chr7.4:76628672-76628691	None:intergenic	35.0%
!!	AAGACTTTCCTGATTTGGTA+AGG	+	chr7.4:76628775-76628794	MS.gene74082:CDS	35.0%
	ACTTTGCATCAGGATAAGGA+AGG	-	chr7.4:76628662-76628681	None:intergenic	40.0%
	ATGAATCAAGGATCACTCTC+CGG	+	chr7.4:76628451-76628470	MS.gene74082:CDS	40.0%
	CAAAGACTGTTGGAAAAGGT+TGG	+	chr7.4:76628834-76628853	MS.gene74082:CDS	40.0%
	CATCACTTTCTTTGAATGGC+CGG	-	chr7.4:76628473-76628492	None:intergenic	40.0%
	CGATGAAATTCCTCATGCTA+AGG	+	chr7.4:76628941-76628960	MS.gene74082:CDS	40.0%
	GCAGATTTAGGCTATGCTAT+TGG	+	chr7.4:76628687-76628706	MS.gene74082:CDS	40.0%
	GCTATTGGGTTCAACTATTG+GGG	+	chr7.4:76628702-76628721	MS.gene74082:CDS	40.0%
!	ATGCTGACGATGTGTTATTG+TGG	+	chr7.4:76628490-76628509	MS.gene74082:CDS	40.0%
!	TGCTGACGATGTGTTATTGT+GGG	+	chr7.4:76628491-76628510	MS.gene74082:CDS	40.0%
!!	GGAAAAGGTTGGTTTCCAAA+GGG	+	chr7.4:76628845-76628864	MS.gene74082:CDS	40.0%
!!	TGGAAAAGGTTGGTTTCCAA+AGG	+	chr7.4:76628844-76628863	MS.gene74082:CDS	40.0%
	AAGGGAGGGATTGTTCAGAA+AGG	+	chr7.4:76628863-76628882	MS.gene74082:CDS	45.0%
	AGGGAGGGATTGTTCAGAAA+GGG	+	chr7.4:76628864-76628883	MS.gene74082:CDS	45.0%
	CATGGCTTCTCAAAGACTGT+TGG	+	chr7.4:76628824-76628843	MS.gene74082:CDS	45.0%
	TAATACTCGTCCGGAGAACA+TGG	+	chr7.4:76628806-76628825	MS.gene74082:CDS	45.0%
	TTCTGAACAATCCCTCCCTT+TGG	-	chr7.4:76628863-76628882	None:intergenic	45.0%
!	CTTTGAGAAGCCATGTTCTC+CGG	-	chr7.4:76628819-76628838	None:intergenic	45.0%
!!	AAAGGTTGGTTTCCAAAGGG+AGG	+	chr7.4:76628848-76628867	MS.gene74082:CDS	45.0%
!!	AAGGTTGGTTTCCAAAGGGA+GGG	+	chr7.4:76628849-76628868	MS.gene74082:CDS	45.0%
!!	TCCTGATTTGGTAAGGTTGC+AGG	+	chr7.4:76628782-76628801	MS.gene74082:CDS	45.0%
	GCCTGCAACCTTACCAAATC+AGG	-	chr7.4:76628786-76628805	None:intergenic	50.0%
	GTTGCAGGCTAATACTCGTC+CGG	+	chr7.4:76628797-76628816	MS.gene74082:CDS	50.0%
!	CTGCTTTGGTAGCTACACCT+TGG	-	chr7.4:76628728-76628747	None:intergenic	50.0%
	GGGTTCAACTATTGGGGCCA+AGG	+	chr7.4:76628708-76628727	MS.gene74082:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	76628438	76628968	76628438	ID=MS.gene74082
chr7.4	mRNA	76628438	76628968	76628438	ID=MS.gene74082.t1;Parent=MS.gene74082
chr7.4	exon	76628438	76628968	76628438	ID=MS.gene74082.t1.exon1;Parent=MS.gene74082.t1
chr7.4	CDS	76628438	76628968	76628438	ID=cds.MS.gene74082.t1;Parent=MS.gene74082.t1

Gene Sequence

>MS.gene74082

ATGGGAATAGATCATGAATCAAGGATCACTCTCCGGCCATTCAAAGAAAGTGATGCTGACGATGTGTTATTGTGGGCAAGTGATGAAAGAGTCATGCAATATATGCGATTGGAGTTATGCAAATCAAGGGAAGAAGCTTTGAATTTCATAAAAAATGAATGCATTTATCCATTCCAACTATCAATTTGTCTTAATGATCACTCTATTGGAATTGTTTTTGTCCTTCCTTATCCTGATGCAAAGTTTAAAGCAGATTTAGGCTATGCTATTGGGTTCAACTATTGGGGCCAAGGTGTAGCTACCAAAGCAGTGAAGATTCTTTTGTCTCGTGTATTCCAAGACTTTCCTGATTTGGTAAGGTTGCAGGCTAATACTCGTCCGGAGAACATGGCTTCTCAAAGACTGTTGGAAAAGGTTGGTTTCCAAAGGGAGGGATTGTTCAGAAAGGGAATTTATAACAAAGGGAATGTAGAGGATTTCTGTATTTTTAGTTTCTTGTCTACCGATGAAATTCCTCATGCTAAGGGTTAA

Protein sequence

>MS.gene74082.t1

MGIDHESRITLRPFKESDADDVLLWASDERVMQYMRLELCKSREEALNFIKNECIYPFQLSICLNDHSIGIVFVLPYPDAKFKADLGYAIGFNYWGQGVATKAVKILLSRVFQDFPDLVRLQANTRPENMASQRLLEKVGFQREGLFRKGIYNKGNVEDFCIFSFLSTDEIPHAKG