AlfalfaGEDB | MS.gene57210

M. sativa cultivar XinJiangDaYe / MS.gene57210

KEGG: K17428
GO: GO:0000313 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005761 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF06984.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57210.t1	XP_003611613.2	97.8	137	2	1	1	136	1	137	7.30E-67	263.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57210.t1	Q8K2Y7	50.6	89	43	1	29	116	63	151	2.1e-14	80.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57210.t1	G7K3V1	97.8	137	2	1	1	136	1	137	5.2e-67	263.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049166	MS.gene57210	0.808582	2.89E-50	-1.69E-46
MS.gene049255	MS.gene57210	0.846561	2.01E-59	-1.69E-46
MS.gene049341	MS.gene57210	0.812055	5.14E-51	-1.69E-46
MS.gene049580	MS.gene57210	0.802296	6.00E-49	-1.69E-46
MS.gene049756	MS.gene57210	0.846194	2.53E-59	-1.69E-46
MS.gene050085	MS.gene57210	0.803728	3.04E-49	-1.69E-46
MS.gene050277	MS.gene57210	0.899969	1.17E-77	-1.69E-46
MS.gene050303	MS.gene57210	0.829391	5.29E-55	-1.69E-46
MS.gene050317	MS.gene57210	0.841751	3.93E-58	-1.69E-46
MS.gene050320	MS.gene57210	0.840745	7.22E-58	-1.69E-46
MS.gene050437	MS.gene57210	0.828754	7.54E-55	-1.69E-46
MS.gene050752	MS.gene57210	0.837611	4.69E-57	-1.69E-46
MS.gene050815	MS.gene57210	0.816084	6.63E-52	-1.69E-46
MS.gene050838	MS.gene57210	0.830481	2.87E-55	-1.69E-46
MS.gene050955	MS.gene57210	0.816795	4.60E-52	-1.69E-46
MS.gene051003	MS.gene57210	0.828101	1.08E-54	-1.69E-46
MS.gene051063	MS.gene57210	0.822728	2.03E-53	-1.69E-46
MS.gene051241	MS.gene57210	0.804099	2.54E-49	-1.69E-46
MS.gene051243	MS.gene57210	0.815653	8.28E-52	-1.69E-46
MS.gene051245	MS.gene57210	0.842512	2.47E-58	-1.69E-46
MS.gene051328	MS.gene57210	0.817175	3.78E-52	-1.69E-46
MS.gene051330	MS.gene57210	0.838424	2.90E-57	-1.69E-46
MS.gene051343	MS.gene57210	0.808046	3.75E-50	-1.69E-46
MS.gene05134	MS.gene57210	0.841106	5.81E-58	-1.69E-46
MS.gene051451	MS.gene57210	0.85226	5.19E-61	-1.69E-46
MS.gene051633	MS.gene57210	0.80418	2.44E-49	-1.69E-46
MS.gene051700	MS.gene57210	0.805056	1.60E-49	-1.69E-46
MS.gene051746	MS.gene57210	0.852984	3.22E-61	-1.69E-46
MS.gene051748	MS.gene57210	0.854043	1.60E-61	-1.69E-46
MS.gene051756	MS.gene57210	0.83036	3.07E-55	-1.69E-46
MS.gene051757	MS.gene57210	0.82763	1.41E-54	-1.69E-46
MS.gene051850	MS.gene57210	0.810043	1.40E-50	-1.69E-46
MS.gene05188	MS.gene57210	0.8337	4.59E-56	-1.69E-46
MS.gene051903	MS.gene57210	0.865915	4.18E-65	-1.69E-46
MS.gene051975	MS.gene57210	0.831181	1.93E-55	-1.69E-46
MS.gene052133	MS.gene57210	0.809873	1.53E-50	-1.69E-46
MS.gene052286	MS.gene57210	0.828899	6.96E-55	-1.69E-46
MS.gene052302	MS.gene57210	0.803002	4.29E-49	-1.69E-46
MS.gene052376	MS.gene57210	0.802534	5.36E-49	-1.69E-46
MS.gene052856	MS.gene57210	0.807389	5.18E-50	-1.69E-46
MS.gene052947	MS.gene57210	0.855677	5.37E-62	-1.69E-46
MS.gene053058	MS.gene57210	0.819284	1.26E-52	-1.69E-46
MS.gene053109	MS.gene57210	0.874719	5.42E-68	-1.69E-46
MS.gene053381	MS.gene57210	0.804643	1.96E-49	-1.69E-46
MS.gene053952	MS.gene57210	0.809593	1.75E-50	-1.69E-46
MS.gene054403	MS.gene57210	0.833868	4.16E-56	-1.69E-46
MS.gene055162	MS.gene57210	0.812203	4.77E-51	-1.69E-46
MS.gene055424	MS.gene57210	0.827601	1.43E-54	-1.69E-46
MS.gene055441	MS.gene57210	0.833146	6.31E-56	-1.69E-46
MS.gene055512	MS.gene57210	0.804961	1.68E-49	-1.69E-46
MS.gene056432	MS.gene57210	0.828636	8.05E-55	-1.69E-46
MS.gene056580	MS.gene57210	0.847923	8.50E-60	-1.69E-46
MS.gene056601	MS.gene57210	0.831423	1.68E-55	-1.69E-46
MS.gene056631	MS.gene57210	0.893839	4.33E-75	-1.69E-46
MS.gene056752	MS.gene57210	0.809044	2.30E-50	-1.69E-46
MS.gene056760	MS.gene57210	0.82207	2.88E-53	-1.69E-46
MS.gene056787	MS.gene57210	0.816116	6.53E-52	-1.69E-46
MS.gene056986	MS.gene57210	0.829785	4.24E-55	-1.69E-46
MS.gene057057	MS.gene57210	0.846624	1.93E-59	-1.69E-46
MS.gene057094	MS.gene57210	0.871665	5.75E-67	-1.69E-46
MS.gene057140	MS.gene57210	0.843959	1.02E-58	-1.69E-46
MS.gene057234	MS.gene57210	0.877805	4.68E-69	-1.69E-46
MS.gene057705	MS.gene57210	0.834139	3.56E-56	-1.69E-46
MS.gene05781	MS.gene57210	0.808137	3.59E-50	-1.69E-46
MS.gene057999	MS.gene57210	0.826027	3.40E-54	-1.69E-46
MS.gene058054	MS.gene57210	0.816592	5.11E-52	-1.69E-46
MS.gene058055	MS.gene57210	0.848813	4.83E-60	-1.69E-46
MS.gene058238	MS.gene57210	0.899057	2.89E-77	-1.69E-46
MS.gene058654	MS.gene57210	0.806907	6.55E-50	-1.69E-46
MS.gene058906	MS.gene57210	0.866618	2.50E-65	-1.69E-46
MS.gene059146	MS.gene57210	0.810159	1.32E-50	-1.69E-46
MS.gene059317	MS.gene57210	0.808192	3.49E-50	-1.69E-46
MS.gene059830	MS.gene57210	0.836156	1.10E-56	-1.69E-46
MS.gene059898	MS.gene57210	0.842491	2.50E-58	-1.69E-46
MS.gene06001	MS.gene57210	0.881561	2.17E-70	-1.69E-46
MS.gene06011	MS.gene57210	0.86687	2.08E-65	-1.69E-46
MS.gene060429	MS.gene57210	0.819855	9.33E-53	-1.69E-46
MS.gene060463	MS.gene57210	0.817105	3.92E-52	-1.69E-46
MS.gene060465	MS.gene57210	0.818358	2.04E-52	-1.69E-46
MS.gene060586	MS.gene57210	0.852232	5.28E-61	-1.69E-46
MS.gene060702	MS.gene57210	0.811846	5.71E-51	-1.69E-46
MS.gene060763	MS.gene57210	0.831589	1.53E-55	-1.69E-46
MS.gene060830	MS.gene57210	0.807008	6.23E-50	-1.69E-46
MS.gene061145	MS.gene57210	0.823817	1.13E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene57210	MS.gene005865	PPI
MS.gene57210	MS.gene066059	PPI
MS.gene57210	MS.gene21110	PPI
MS.gene57210	MS.gene21227	PPI
MS.gene57210	MS.gene036564	PPI
MS.gene57210	MS.gene026968	PPI
MS.gene34167	MS.gene57210	PPI
MS.gene57210	MS.gene028903	PPI
MS.gene21111	MS.gene57210	PPI
MS.gene60589	MS.gene57210	PPI
MS.gene57210	MS.gene055808	PPI
MS.gene57210	MS.gene035256	PPI
MS.gene57210	MS.gene20938	PPI
MS.gene57210	MS.gene82915	PPI
MS.gene005399	MS.gene57210	PPI
MS.gene57210	MS.gene001687	PPI
MS.gene57210	MS.gene043435	PPI
MS.gene31468	MS.gene57210	PPI
MS.gene57210	MS.gene60861	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57210.t1	MTR_5g015850	97.810	137	2	1	1	136	1	137	6.36e-97	275

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57210.t1	AT1G07830	71.528	144	33	3	1	136	1	144	4.39e-67	199

Find 25 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGAATTGCGTCTGAAATCT+TGG	0.295639	5.3:-8728230	MS.gene57210:CDS
CCCTCTTCCGGAGTTCTTTG+AGG	0.397007	5.3:-8728445	MS.gene57210:CDS
GAGATGAAGAAAATGATAAA+TGG	0.401349	5.3:-8727773	MS.gene57210:CDS
TTAGGTAAGCGCTCCGGGTT+AGG	0.401390	5.3:+8728114	None:intergenic
GTTGTGGTATGTGTTGTTAA+AGG	0.420053	5.3:-8728193	MS.gene57210:CDS
AACGAGACACCAGTTGTCTA+TGG	0.421926	5.3:-8728411	MS.gene57210:intron
TGAATTGCGTCTGAAATCTT+GGG	0.436291	5.3:-8728229	MS.gene57210:CDS
TCAGCAGACCTCCTCGCGTC+TGG	0.436837	5.3:+8727794	None:intergenic
TGCTGTTAGAAACCCTCTTC+CGG	0.440920	5.3:-8728457	MS.gene57210:CDS
ATACCTTAGGTAAGCGCTCC+GGG	0.470522	5.3:+8728109	None:intergenic
GAACTTGCGCTTTCCTAACC+CGG	0.481233	5.3:-8728127	MS.gene57210:CDS
GCCTCAAAGAACTCCGGAAG+AGG	0.493349	5.3:+8728444	None:intergenic
TATTTCATACCATAGACAAC+TGG	0.503485	5.3:+8728402	None:intergenic
AATACCTTAGGTAAGCGCTC+CGG	0.514447	5.3:+8728108	None:intergenic
AAACAGTATTCCACTACAGC+TGG	0.543067	5.3:-8728480	MS.gene57210:CDS
ACACATACCACAACTTATGA+AGG	0.545630	5.3:+8728202	None:intergenic
TTTGTTAGGTCGGAGTTGGA+AGG	0.551769	5.3:-8728256	MS.gene57210:intron
GGGATGACCTTCATAAGTTG+TGG	0.556409	5.3:-8728209	MS.gene57210:CDS
TCTAACAGCACCAGCTGTAG+TGG	0.557909	5.3:+8728470	None:intergenic
TAACCCGGAGCGCTTACCTA+AGG	0.570629	5.3:-8728112	MS.gene57210:intron
CTGTCTGCCTCAAAGAACTC+CGG	0.578032	5.3:+8728438	None:intergenic
CCTCAAAGAACTCCGGAAGA+GGG	0.599992	5.3:+8728445	None:intergenic
TTGAAGAACCAGACGCGAGG+AGG	0.633013	5.3:-8727802	MS.gene57210:CDS
CAGTAAGCACATGCTTGATG+CGG	0.642610	5.3:+8727834	None:intergenic
CGATTGAAGAACCAGACGCG+AGG	0.687088	5.3:-8727805	MS.gene57210:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATGTTAAATTTCGTAT+GGG	-	chr5.3:8728365-8728384	MS.gene57210:intron	15.0%
!!	GTAAAATGTTAAATTTCGTA+TGG	-	chr5.3:8728364-8728383	MS.gene57210:intron	20.0%
!!!	ATAGTTTTACCATTTTTGTT+AGG	-	chr5.3:8728030-8728049	MS.gene57210:intron	20.0%
!!!	GATTTAATTTATGTGAAATC+AGG	-	chr5.3:8728427-8728446	MS.gene57210:CDS	20.0%
!	AAAAAGTGAAGAAATACCTT+AGG	+	chr5.3:8728207-8728226	None:intergenic	25.0%
!	GAGATGAAGAAAATGATAAA+TGG	-	chr5.3:8728527-8728546	MS.gene57210:CDS	25.0%
!	TATAAGAAAACAAAGTGATG+AGG	+	chr5.3:8728013-8728032	None:intergenic	25.0%
!!!	ATTTTTTTGTACCTAATGTG+TGG	+	chr5.3:8727981-8728000	None:intergenic	25.0%
!!!	TTTTACCATTTTTGTTAGGT+CGG	-	chr5.3:8728034-8728053	MS.gene57210:intron	25.0%
	AGTTATGTGGACATTGATAT+AGG	-	chr5.3:8728267-8728286	MS.gene57210:intron	30.0%
	ATCAATGTCCACATAACTAA+GGG	+	chr5.3:8728265-8728284	None:intergenic	30.0%
	ATGTTAAATTTCGTATGGGT+TGG	-	chr5.3:8728369-8728388	MS.gene57210:intron	30.0%
	CCTATTCTATTGAAAAGAAG+AGG	+	chr5.3:8727928-8727947	None:intergenic	30.0%
	TATCAATGTCCACATAACTA+AGG	+	chr5.3:8728266-8728285	None:intergenic	30.0%
	TATTTCATACCATAGACAAC+TGG	+	chr5.3:8727901-8727920	None:intergenic	30.0%
	TGTTAAATTTCGTATGGGTT+GGG	-	chr5.3:8728370-8728389	MS.gene57210:intron	30.0%
	TTTCATTATAGCCACACATT+AGG	-	chr5.3:8727967-8727986	MS.gene57210:intron	30.0%
!	CCTCTTCTTTTCAATAGAAT+AGG	-	chr5.3:8727925-8727944	MS.gene57210:intron	30.0%
!	TTTTGTTACCCTTAGTTATG+TGG	-	chr5.3:8728254-8728273	MS.gene57210:intron	30.0%
!!!	ATTTCTTCACTTTTTACACC+TGG	-	chr5.3:8728212-8728231	MS.gene57210:CDS	30.0%
	ACACATACCACAACTTATGA+AGG	+	chr5.3:8728101-8728120	None:intergenic	35.0%
	ATTGATATAGGAAATCGCAG+TGG	-	chr5.3:8728279-8728298	MS.gene57210:intron	35.0%
!	GTTAAATTTCGTATGGGTTG+GGG	-	chr5.3:8728371-8728390	MS.gene57210:intron	35.0%
!	GTTGTGGTATGTGTTGTTAA+AGG	-	chr5.3:8728107-8728126	MS.gene57210:intron	35.0%
!	TGAATTGCGTCTGAAATCTT+GGG	-	chr5.3:8728071-8728090	MS.gene57210:intron	35.0%
!!	TTAAATTTCGTATGGGTTGG+GGG	-	chr5.3:8728372-8728391	MS.gene57210:intron	35.0%
!!!	AGTGGAATACTGTTTTGATC+TGG	+	chr5.3:8727815-8727834	None:intergenic	35.0%
	AAACAGTATTCCACTACAGC+TGG	-	chr5.3:8727820-8727839	MS.gene57210:CDS	40.0%
	CAACTCCGACCTAACAAAAA+TGG	+	chr5.3:8728042-8728061	None:intergenic	40.0%
!	CATTTTTGTTAGGTCGGAGT+TGG	-	chr5.3:8728040-8728059	MS.gene57210:intron	40.0%
!	CTGAATTGCGTCTGAAATCT+TGG	-	chr5.3:8728070-8728089	MS.gene57210:intron	40.0%
!	GTGGTGCTTTTATCAACGTT+TGG	-	chr5.3:8728301-8728320	MS.gene57210:intron	40.0%
!!	TAAATTTCGTATGGGTTGGG+GGG	-	chr5.3:8728373-8728392	MS.gene57210:intron	40.0%
	AACGAGACACCAGTTGTCTA+TGG	-	chr5.3:8727889-8727908	MS.gene57210:intron	45.0%
	AATACCTTAGGTAAGCGCTC+CGG	+	chr5.3:8728195-8728214	None:intergenic	45.0%
	CAGTAAGCACATGCTTGATG+CGG	+	chr5.3:8728469-8728488	None:intergenic	45.0%
	GATATAGGAAATCGCAGTGG+TGG	-	chr5.3:8728282-8728301	MS.gene57210:intron	45.0%
	GGGATGACCTTCATAAGTTG+TGG	-	chr5.3:8728091-8728110	MS.gene57210:intron	45.0%
	TGCTGTTAGAAACCCTCTTC+CGG	-	chr5.3:8727843-8727862	MS.gene57210:CDS	45.0%
	TTTGTTAGGTCGGAGTTGGA+AGG	-	chr5.3:8728044-8728063	MS.gene57210:intron	45.0%
	ATACCTTAGGTAAGCGCTCC+GGG	+	chr5.3:8728194-8728213	None:intergenic	50.0%
	CAACACGCGTAAATCAGACC+AGG	+	chr5.3:8728233-8728252	None:intergenic	50.0%
	CCTCAAAGAACTCCGGAAGA+GGG	+	chr5.3:8727858-8727877	None:intergenic	50.0%
	CTGTCTGCCTCAAAGAACTC+CGG	+	chr5.3:8727865-8727884	None:intergenic	50.0%
	GAACTTGCGCTTTCCTAACC+CGG	-	chr5.3:8728173-8728192	MS.gene57210:CDS	50.0%
	TCTAACAGCACCAGCTGTAG+TGG	+	chr5.3:8727833-8727852	None:intergenic	50.0%
	CCCTCTTCCGGAGTTCTTTG+AGG	-	chr5.3:8727855-8727874	MS.gene57210:intron	55.0%
	CGATTGAAGAACCAGACGCG+AGG	-	chr5.3:8728495-8728514	MS.gene57210:CDS	55.0%
	GCCTCAAAGAACTCCGGAAG+AGG	+	chr5.3:8727859-8727878	None:intergenic	55.0%
	GGTTGGGGGGAAACTGTTCA+CGG	-	chr5.3:8728386-8728405	MS.gene57210:intron	55.0%
	TTAGGTAAGCGCTCCGGGTT+AGG	+	chr5.3:8728189-8728208	None:intergenic	55.0%
	TTGAAGAACCAGACGCGAGG+AGG	-	chr5.3:8728498-8728517	MS.gene57210:CDS	55.0%
!	TAACCCGGAGCGCTTACCTA+AGG	-	chr5.3:8728188-8728207	MS.gene57210:CDS	55.0%
	TCAGCAGACCTCCTCGCGTC+TGG	+	chr5.3:8728509-8728528	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	8727766	8728556	8727766	ID=MS.gene57210
chr5.3	mRNA	8727766	8728556	8727766	ID=MS.gene57210.t1;Parent=MS.gene57210
chr5.3	exon	8728412	8728556	8728412	ID=MS.gene57210.t1.exon1;Parent=MS.gene57210.t1
chr5.3	CDS	8728412	8728556	8728412	ID=cds.MS.gene57210.t1;Parent=MS.gene57210.t1
chr5.3	exon	8728113	8728270	8728113	ID=MS.gene57210.t1.exon2;Parent=MS.gene57210.t1
chr5.3	CDS	8728113	8728270	8728113	ID=cds.MS.gene57210.t1;Parent=MS.gene57210.t1
chr5.3	exon	8727766	8727873	8727766	ID=MS.gene57210.t1.exon3;Parent=MS.gene57210.t1
chr5.3	CDS	8727766	8727873	8727766	ID=cds.MS.gene57210.t1;Parent=MS.gene57210.t1

Gene Sequence

>MS.gene57210

ATGTTTTTGGCAAGAGCATTTCGACAATCATTCTTTGCTGCTGCTGCCAGATCAAAACAGTATTCCACTACAGCTGGTGCTGTTAGAAACCCTCTTCCGGAGTTCTTTGAGGCAGACAGAGATAACGAGACACCAGTTGTCTATGGTATGAAATATGCACCTCTTCTTTTCAATAGAATAGGAATTAAAAATCTCACTGATTTTCATTATAGCCACACATTAGGTACAAAAAAATTATAATTTTCCTCATCACTTTGTTTTCTTATAGTTTTACCATTTTTGTTAGGTCGGAGTTGGAAGGCTTCTGAATTGCGTCTGAAATCTTGGGATGACCTTCATAAGTTGTGGTATGTGTTGTTAAAGGAAAAGAATATGTTGATGACTCAACGACAAATGCTTCAAGCTCAGAACTTGCGCTTTCCTAACCCGGAGCGCTTACCTAAGGTATTTCTTCACTTTTTACACCTGGTCTGATTTACGCGTGTTGTTTTTGTTACCCTTAGTTATGTGGACATTGATATAGGAAATCGCAGTGGTGGTGCTTTTATCAACGTTTGGTGAACACTTTTTGTTTTAGAACATACGAGAGAAATTGAATGTAAAATGTTAAATTTCGTATGGGTTGGGGGGAAACTGTTCACGGTTAACAATGATTTTTGTTGATTTAATTTATGTGAAATCAGGTGAGAAAGTCAATGTGCCGCATCAAGCATGTGCTTACTGAGAGAGCGATTGAAGAACCAGACGCGAGGAGGTCTGCTGAGATGAAGAAAATGATAAATGGTCTTTGA

Protein sequence

>MS.gene57210.t1

MFLARAFRQSFFAAAARSKQYSTTAGAVRNPLPEFFEADRDNETPVVYGRSWKASELRLKSWDDLHKLWYVLLKEKNMLMTQRQMLQAQNLRFPNPERLPKVRKSMCRIKHVLTERAIEEPDARRSAEMKKMINGL