Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83476.t1 | RHN54641.1 | 97.6 | 702 | 17 | 0 | 1 | 702 | 1 | 702 | 0.00E+00 | 1279.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83476.t1 | Q9FT73 | 73.8 | 665 | 164 | 2 | 43 | 701 | 43 | 703 | 5.5e-284 | 978.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83476.t1 | A0A396HQ63 | 97.6 | 702 | 17 | 0 | 1 | 702 | 1 | 702 | 0.0e+00 | 1279.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene83476 | 0.824051 | 9.95E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene83476 | 0.809156 | 2.17E-50 | -1.69E-46 |
| MS.gene049501 | MS.gene83476 | 0.809965 | 1.46E-50 | -1.69E-46 |
| MS.gene049647 | MS.gene83476 | 0.804956 | 1.68E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene83476 | 0.810403 | 1.17E-50 | -1.69E-46 |
| MS.gene050156 | MS.gene83476 | 0.800489 | 1.41E-48 | -1.69E-46 |
| MS.gene050226 | MS.gene83476 | 0.802155 | 6.42E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene83476 | 0.814875 | 1.23E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene83476 | 0.817488 | 3.21E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene83476 | 0.839428 | 1.59E-57 | -1.69E-46 |
| MS.gene050719 | MS.gene83476 | 0.824939 | 6.15E-54 | -1.69E-46 |
| MS.gene050721 | MS.gene83476 | 0.825008 | 5.92E-54 | -1.69E-46 |
| MS.gene050724 | MS.gene83476 | 0.810992 | 8.75E-51 | -1.69E-46 |
| MS.gene050787 | MS.gene83476 | 0.818476 | 1.92E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene83476 | 0.81308 | 3.07E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene83476 | 0.802662 | 5.04E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene83476 | 0.822135 | 2.78E-53 | -1.69E-46 |
| MS.gene050959 | MS.gene83476 | 0.83584 | 1.33E-56 | -1.69E-46 |
| MS.gene051247 | MS.gene83476 | 0.802812 | 4.70E-49 | -1.69E-46 |
| MS.gene051265 | MS.gene83476 | 0.821203 | 4.57E-53 | -1.69E-46 |
| MS.gene052020 | MS.gene83476 | 0.849822 | 2.53E-60 | -1.69E-46 |
| MS.gene052323 | MS.gene83476 | 0.806001 | 1.02E-49 | -1.69E-46 |
| MS.gene052384 | MS.gene83476 | 0.812024 | 5.22E-51 | -1.69E-46 |
| MS.gene052613 | MS.gene83476 | 0.813201 | 2.89E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene83476 | 0.819647 | 1.04E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene83476 | 0.818378 | 2.02E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene83476 | 0.816168 | 6.36E-52 | -1.69E-46 |
| MS.gene053221 | MS.gene83476 | 0.83294 | 7.10E-56 | -1.69E-46 |
| MS.gene05414 | MS.gene83476 | 0.800593 | 1.34E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene83476 | 0.806506 | 7.96E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene83476 | 0.829872 | 4.04E-55 | -1.69E-46 |
| MS.gene054468 | MS.gene83476 | 0.82204 | 2.93E-53 | -1.69E-46 |
| MS.gene054472 | MS.gene83476 | 0.813965 | 1.96E-51 | -1.69E-46 |
| MS.gene05462 | MS.gene83476 | 0.813007 | 3.18E-51 | -1.69E-46 |
| MS.gene05468 | MS.gene83476 | 0.818206 | 2.21E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene83476 | 0.836185 | 1.09E-56 | -1.69E-46 |
| MS.gene055357 | MS.gene83476 | 0.818042 | 2.41E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene83476 | 0.838557 | 2.68E-57 | -1.69E-46 |
| MS.gene055363 | MS.gene83476 | 0.811192 | 7.92E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene83476 | 0.808717 | 2.70E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene83476 | 0.826398 | 2.77E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene83476 | 0.80589 | 1.07E-49 | -1.69E-46 |
| MS.gene055608 | MS.gene83476 | 0.806082 | 9.78E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene83476 | 0.802268 | 6.08E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene83476 | -0.818571 | 1.83E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene83476 | 0.815646 | 8.31E-52 | -1.69E-46 |
| MS.gene056552 | MS.gene83476 | 0.808123 | 3.62E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene83476 | 0.808102 | 3.65E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene83476 | 0.81563 | 8.38E-52 | -1.69E-46 |
| MS.gene058084 | MS.gene83476 | 0.810085 | 1.37E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene83476 | 0.811682 | 6.20E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene83476 | 0.81298 | 3.23E-51 | -1.69E-46 |
| MS.gene058636 | MS.gene83476 | 0.816926 | 4.30E-52 | -1.69E-46 |
| MS.gene058798 | MS.gene83476 | 0.807142 | 5.84E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene83476 | 0.807762 | 4.31E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene83476 | 0.81304 | 3.13E-51 | -1.69E-46 |
| MS.gene060137 | MS.gene83476 | -0.81053 | 1.10E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene83476 | 0.830563 | 2.74E-55 | -1.69E-46 |
| MS.gene060683 | MS.gene83476 | 0.806391 | 8.42E-50 | -1.69E-46 |
| MS.gene060685 | MS.gene83476 | 0.805593 | 1.24E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene83476 | 0.803308 | 3.71E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene83476 | 0.829917 | 3.94E-55 | -1.69E-46 |
| MS.gene060998 | MS.gene83476 | 0.807754 | 4.33E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene83476 | -0.817845 | 2.67E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene83476 | MS.gene025141 | PPI |
| MS.gene83476 | MS.gene056998 | PPI |
| MS.gene83476 | MS.gene27517 | PPI |
| MS.gene83476 | MS.gene92776 | PPI |
| MS.gene83476 | MS.gene052695 | PPI |
| MS.gene83476 | MS.gene85610 | PPI |
| MS.gene83476 | MS.gene85235 | PPI |
| MS.gene032228 | MS.gene83476 | PPI |
| MS.gene83476 | MS.gene37366 | PPI |
| MS.gene036929 | MS.gene83476 | PPI |
| MS.gene87672 | MS.gene83476 | PPI |
| MS.gene011242 | MS.gene83476 | PPI |
| MS.gene83476 | MS.gene07826 | PPI |
| MS.gene051963 | MS.gene83476 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83476.t1 | MTR_5g026590 | 93.172 | 703 | 29 | 2 | 1 | 702 | 1 | 685 | 0.0 | 1340 |
| MS.gene83476.t1 | MTR_2g006440 | 37.391 | 690 | 358 | 16 | 71 | 690 | 390 | 1075 | 1.20e-132 | 420 |
| MS.gene83476.t1 | MTR_1g083840 | 41.379 | 435 | 231 | 9 | 61 | 475 | 168 | 598 | 2.09e-107 | 339 |
| MS.gene83476.t1 | MTR_2g042440 | 41.324 | 438 | 219 | 8 | 85 | 503 | 38 | 456 | 3.26e-104 | 334 |
| MS.gene83476.t1 | MTR_2g042440 | 41.324 | 438 | 219 | 8 | 85 | 503 | 38 | 456 | 3.56e-104 | 334 |
| MS.gene83476.t1 | MTR_3g063040 | 41.822 | 428 | 220 | 12 | 83 | 498 | 10 | 420 | 3.91e-93 | 309 |
| MS.gene83476.t1 | MTR_8g080160 | 37.537 | 341 | 191 | 9 | 84 | 417 | 271 | 596 | 5.43e-51 | 191 |
| MS.gene83476.t1 | MTR_1g083250 | 39.583 | 240 | 125 | 6 | 61 | 289 | 136 | 366 | 2.17e-43 | 166 |
| MS.gene83476.t1 | MTR_1g083250 | 41.346 | 104 | 61 | 0 | 317 | 420 | 510 | 613 | 2.64e-20 | 95.9 |
| MS.gene83476.t1 | MTR_1g083250 | 39.583 | 240 | 125 | 6 | 61 | 289 | 136 | 366 | 1.08e-42 | 166 |
| MS.gene83476.t1 | MTR_1g083250 | 36.364 | 165 | 97 | 4 | 317 | 475 | 510 | 672 | 3.77e-25 | 111 |
| MS.gene83476.t1 | MTR_1g083250 | 39.583 | 240 | 125 | 6 | 61 | 289 | 136 | 366 | 1.72e-42 | 166 |
| MS.gene83476.t1 | MTR_1g083250 | 36.364 | 165 | 97 | 4 | 317 | 475 | 510 | 672 | 4.09e-25 | 111 |
| MS.gene83476.t1 | MTR_2g100230 | 23.894 | 339 | 211 | 9 | 102 | 417 | 130 | 444 | 1.00e-13 | 74.7 |
| MS.gene83476.t1 | MTR_4g105100 | 23.881 | 335 | 216 | 12 | 102 | 417 | 236 | 550 | 1.54e-12 | 71.2 |
| MS.gene83476.t1 | MTR_1g026830 | 21.262 | 428 | 265 | 14 | 86 | 479 | 165 | 554 | 2.69e-12 | 70.5 |
| MS.gene83476.t1 | MTR_2g032630 | 26.496 | 351 | 218 | 15 | 86 | 417 | 514 | 843 | 1.66e-11 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83476.t1 | AT1G31360 | 72.702 | 707 | 183 | 2 | 1 | 701 | 1 | 703 | 0.0 | 1085 |
| MS.gene83476.t1 | AT1G31360 | 72.702 | 707 | 183 | 2 | 1 | 701 | 35 | 737 | 0.0 | 1085 |
| MS.gene83476.t1 | AT1G31360 | 75.272 | 643 | 155 | 1 | 1 | 643 | 1 | 639 | 0.0 | 1030 |
| MS.gene83476.t1 | AT1G31360 | 74.399 | 582 | 139 | 2 | 126 | 701 | 1 | 578 | 0.0 | 912 |
| MS.gene83476.t1 | AT1G31360 | 76.881 | 545 | 126 | 0 | 1 | 545 | 1 | 545 | 0.0 | 900 |
| MS.gene83476.t1 | AT1G31360 | 78.450 | 529 | 110 | 1 | 1 | 529 | 1 | 525 | 0.0 | 882 |
| MS.gene83476.t1 | AT1G31360 | 78.585 | 523 | 112 | 0 | 1 | 523 | 1 | 523 | 0.0 | 882 |
| MS.gene83476.t1 | AT1G31360 | 78.585 | 523 | 112 | 0 | 1 | 523 | 1 | 523 | 0.0 | 882 |
| MS.gene83476.t1 | AT1G10930 | 37.031 | 667 | 336 | 14 | 71 | 664 | 435 | 1090 | 1.84e-128 | 409 |
| MS.gene83476.t1 | AT4G35740 | 43.032 | 409 | 214 | 7 | 85 | 481 | 32 | 433 | 6.92e-105 | 335 |
| MS.gene83476.t1 | AT3G05740 | 42.607 | 399 | 212 | 6 | 78 | 463 | 107 | 501 | 4.44e-102 | 322 |
| MS.gene83476.t1 | AT3G05740 | 42.607 | 399 | 212 | 6 | 78 | 463 | 192 | 586 | 3.15e-101 | 323 |
| MS.gene83476.t1 | AT4G35740 | 41.499 | 347 | 184 | 7 | 147 | 481 | 1 | 340 | 9.04e-78 | 261 |
| MS.gene83476.t1 | AT1G27880 | 34.715 | 386 | 228 | 9 | 43 | 417 | 218 | 590 | 5.24e-53 | 197 |
| MS.gene83476.t1 | AT1G27880 | 34.715 | 386 | 228 | 9 | 43 | 417 | 218 | 590 | 9.44e-53 | 196 |
| MS.gene83476.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.09e-43 | 167 |
| MS.gene83476.t1 | AT5G27680 | 30.688 | 189 | 91 | 6 | 317 | 497 | 501 | 657 | 6.53e-14 | 75.9 |
| MS.gene83476.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.33e-43 | 168 |
| MS.gene83476.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.37e-28 | 122 |
| MS.gene83476.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.33e-43 | 168 |
| MS.gene83476.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.37e-28 | 122 |
| MS.gene83476.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.33e-43 | 168 |
| MS.gene83476.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.37e-28 | 122 |
| MS.gene83476.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.33e-43 | 168 |
| MS.gene83476.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.37e-28 | 122 |
| MS.gene83476.t1 | AT5G27680 | 40.435 | 230 | 113 | 6 | 73 | 289 | 22 | 240 | 7.16e-43 | 166 |
| MS.gene83476.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 371 | 554 | 1.35e-28 | 122 |
| MS.gene83476.t1 | AT5G63120 | 22.414 | 348 | 205 | 10 | 102 | 417 | 200 | 514 | 1.81e-12 | 70.9 |
| MS.gene83476.t1 | AT1G55150 | 23.077 | 338 | 215 | 10 | 102 | 417 | 159 | 473 | 4.36e-12 | 69.7 |
| MS.gene83476.t1 | AT1G55150 | 23.077 | 338 | 215 | 10 | 102 | 417 | 134 | 448 | 5.74e-12 | 69.3 |
| MS.gene83476.t1 | AT3G01540 | 24.157 | 356 | 220 | 12 | 86 | 417 | 177 | 506 | 4.95e-11 | 66.6 |
| MS.gene83476.t1 | AT3G01540 | 24.157 | 356 | 220 | 12 | 86 | 417 | 177 | 506 | 4.95e-11 | 66.6 |
| MS.gene83476.t1 | AT3G01540 | 24.157 | 356 | 220 | 12 | 86 | 417 | 177 | 506 | 4.95e-11 | 66.6 |
| MS.gene83476.t1 | AT3G01540 | 24.157 | 356 | 220 | 12 | 86 | 417 | 177 | 506 | 5.12e-11 | 66.2 |
Find 127 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTGAACTAAATTCGATTT+TGG | 0.157671 | 5.4:-17448435 | MS.gene83476:CDS |
| TTCTTTGTTGCAAGATATTC+AGG | 0.234107 | 5.4:-17440764 | MS.gene83476:CDS |
| ACATCTCTACAAGATCATTT+TGG | 0.244904 | 5.4:+17445565 | None:intergenic |
| ACAATCCCTGATTCGTGATT+TGG | 0.255388 | 5.4:+17445284 | None:intergenic |
| TATACCATGTAAAATAAATT+TGG | 0.264948 | 5.4:+17445498 | None:intergenic |
| CTTGCCTCTTCCAGTGAGTT+TGG | 0.267298 | 5.4:+17437042 | None:intergenic |
| TTTACATTTCAGGTAGCTTT+TGG | 0.276488 | 5.4:-17443824 | MS.gene83476:intron |
| ACTTCGACGGCATTGTCTTT+TGG | 0.284094 | 5.4:+17441170 | None:intergenic |
| CCAAAGCACGCTCCAGTAAA+AGG | 0.284767 | 5.4:+17438540 | None:intergenic |
| GTGCAGTTGGAGAAAGTAAT+TGG | 0.285142 | 5.4:-17436589 | MS.gene83476:intron |
| GTGGGGTTGCTTCAGGTAAT+GGG | 0.286772 | 5.4:-17447076 | MS.gene83476:intron |
| ATTCTCAAGCTGATGATCTT+AGG | 0.305060 | 5.4:-17448328 | MS.gene83476:CDS |
| AATAAGAACAAGGTTCTTCT+TGG | 0.307971 | 5.4:+17436450 | None:intergenic |
| AAAGCAACCATAGGAACATT+AGG | 0.308648 | 5.4:+17445717 | None:intergenic |
| AAAACAAAACAGAAGGAAAT+AGG | 0.313249 | 5.4:-17440697 | MS.gene83476:intron |
| ATGGTCTTCTATGAAAATTC+AGG | 0.314044 | 5.4:-17441931 | MS.gene83476:CDS |
| CTCAGCAAAATGCCTGAAAA+AGG | 0.319842 | 5.4:+17441146 | None:intergenic |
| TCTTATCCAAATCACGAATC+AGG | 0.333951 | 5.4:-17445290 | MS.gene83476:CDS |
| CACATCTCTTCCACTCAATA+TGG | 0.350170 | 5.4:+17447310 | None:intergenic |
| CACCAGGTCTGAAGTATAAT+AGG | 0.356483 | 5.4:+17442422 | None:intergenic |
| AAACGCAGCCTCGGGTCATC+TGG | 0.375770 | 5.4:-17437177 | MS.gene83476:CDS |
| GTTCGAGTATTGCGGAGAAT+TGG | 0.379178 | 5.4:-17448370 | MS.gene83476:CDS |
| TGCAGGGCATGCAAAATTGA+TGG | 0.387317 | 5.4:-17440788 | MS.gene83476:intron |
| TGACTTAAAACGAGAGGAAA+TGG | 0.395004 | 5.4:-17438579 | MS.gene83476:CDS |
| GTGTAAAGAATCTGAAAATC+AGG | 0.408828 | 5.4:-17448408 | MS.gene83476:CDS |
| GAAGTTCATATACAAAGCTT+TGG | 0.411764 | 5.4:-17446993 | MS.gene83476:CDS |
| CAGCCTCGGGTCATCTGGCT+TGG | 0.413431 | 5.4:-17437172 | MS.gene83476:CDS |
| GGTAATGGGTTTGACTGCCT+TGG | 0.427589 | 5.4:-17447062 | MS.gene83476:CDS |
| ATTGGAATGGGACGTTTGAA+TGG | 0.433659 | 5.4:-17448352 | MS.gene83476:CDS |
| ATACCAAGGTTCTTGTAATC+AGG | 0.433950 | 5.4:+17445756 | None:intergenic |
| CTCTAGGAAGGAATGTGAAC+AGG | 0.437358 | 5.4:-17445252 | MS.gene83476:intron |
| TGCAAAATTTGCTTCGCCAA+TGG | 0.440678 | 5.4:+17437556 | None:intergenic |
| GCTTTCACTAATTCAAGATC+AGG | 0.442848 | 5.4:-17447195 | MS.gene83476:intron |
| GTAATGGGTTTGACTGCCTT+GGG | 0.449141 | 5.4:-17447061 | MS.gene83476:CDS |
| TCAGTTCCCTAATGTTCCTA+TGG | 0.451520 | 5.4:-17445724 | MS.gene83476:CDS |
| AAACTGAAGACGGAAAAGTA+TGG | 0.463144 | 5.4:-17436565 | MS.gene83476:CDS |
| TTGGAATGGGACGTTTGAAT+GGG | 0.463176 | 5.4:-17448351 | MS.gene83476:CDS |
| AATTTGTCAGCACAGTCAAT+AGG | 0.468637 | 5.4:-17445521 | MS.gene83476:CDS |
| GTCATCATGGCTGCAGGTGG+TGG | 0.469281 | 5.4:-17447284 | MS.gene83476:CDS |
| AAGCAACCATAGGAACATTA+GGG | 0.471975 | 5.4:+17445718 | None:intergenic |
| CTTCCTGCTGTTCTTCGCAA+TGG | 0.476512 | 5.4:-17447242 | MS.gene83476:CDS |
| GTACCATTGCACTCCTGTAA+TGG | 0.481444 | 5.4:+17441123 | None:intergenic |
| GCTAACTTCAACAACAAGTA+AGG | 0.482808 | 5.4:-17447023 | MS.gene83476:CDS |
| TTTAGGTTGCAAAGGAGCTA+AGG | 0.491708 | 5.4:-17444610 | MS.gene83476:intron |
| AAAGAACTTTCTTCCACTCA+TGG | 0.494065 | 5.4:-17437126 | MS.gene83476:CDS |
| AGTGGTCGAGCTGGTCGAGA+TGG | 0.494642 | 5.4:-17442460 | MS.gene83476:CDS |
| TCACAGCTTGAGTAAATCAA+TGG | 0.495744 | 5.4:-17442893 | MS.gene83476:CDS |
| AAAACCAAACTCACTGGAAG+AGG | 0.507726 | 5.4:-17437046 | MS.gene83476:intron |
| TGGTCATTATCATGCAGATA+TGG | 0.508007 | 5.4:-17444570 | MS.gene83476:CDS |
| TTACATTACTGTGGGGCCAT+TGG | 0.508759 | 5.4:-17437572 | MS.gene83476:CDS |
| GCTGAAATCGAAGATGTTCA+AGG | 0.509003 | 5.4:-17448608 | MS.gene83476:intron |
| CGACCTGATTACAAGAACCT+TGG | 0.510980 | 5.4:-17445759 | MS.gene83476:CDS |
| GGAAAAGTCATCCGTCAGTA+AGG | 0.511037 | 5.4:-17445348 | MS.gene83476:CDS |
| AACATTACTTTAGGTTGCAA+AGG | 0.512532 | 5.4:-17444618 | MS.gene83476:intron |
| TCAGACCTGGTGATGTCCCC+CGG | 0.513480 | 5.4:-17442411 | MS.gene83476:CDS |
| CAAAGCTTTGGAGAAGGGCG+AGG | 0.515095 | 5.4:-17446981 | MS.gene83476:CDS |
| TGACATAGTACGATATTGTC+AGG | 0.518563 | 5.4:-17441893 | MS.gene83476:intron |
| TAAGATTGAAACGCAGCCTC+GGG | 0.520222 | 5.4:-17437185 | MS.gene83476:CDS |
| TTGATTTACTCAAGCTGTGA+TGG | 0.520373 | 5.4:+17442896 | None:intergenic |
| ATGATGGTAGAGCTGCAAAA+AGG | 0.524588 | 5.4:-17436480 | MS.gene83476:CDS |
| TGCGAAGAACAGCAGGAAGC+TGG | 0.525627 | 5.4:+17447246 | None:intergenic |
| AGTATTGCGGAGAATTGGAA+TGG | 0.526409 | 5.4:-17448365 | MS.gene83476:CDS |
| ACAAATGCTTACATTACTGT+GGG | 0.528063 | 5.4:-17437580 | MS.gene83476:CDS |
| TGTTTATTGCTTCTCTAGGA+AGG | 0.528457 | 5.4:-17445264 | MS.gene83476:CDS |
| CAACAGGAAAGTGGTCGAGC+TGG | 0.528486 | 5.4:-17442469 | MS.gene83476:intron |
| GGCACATTGCTGTAGTCAGT+GGG | 0.529118 | 5.4:-17445793 | MS.gene83476:CDS |
| ACTCCATTGCGAAGAACAGC+AGG | 0.530357 | 5.4:+17447239 | None:intergenic |
| GTAAGATTGAAACGCAGCCT+CGG | 0.535718 | 5.4:-17437186 | MS.gene83476:CDS |
| AAAGCTTTGGAGAAGGGCGA+GGG | 0.537834 | 5.4:-17446980 | MS.gene83476:CDS |
| TACAAATGCTTACATTACTG+TGG | 0.538641 | 5.4:-17437581 | MS.gene83476:CDS |
| AACAAGCTGCAGGTCATAGT+TGG | 0.538884 | 5.4:-17443942 | MS.gene83476:CDS |
| GTGGTCGAGCTGGTCGAGAT+GGG | 0.541598 | 5.4:-17442459 | MS.gene83476:CDS |
| GGATTGTTTATTGCTTCTCT+AGG | 0.542756 | 5.4:-17445268 | MS.gene83476:CDS |
| ATTATTAATGCCATATTGAG+TGG | 0.542763 | 5.4:-17447320 | MS.gene83476:CDS |
| GTGCTGGTCATCATGGCTGC+AGG | 0.544531 | 5.4:-17447290 | MS.gene83476:CDS |
| TTGAAGTTAGCATATATGCT+GGG | 0.553613 | 5.4:+17447035 | None:intergenic |
| ATATACAAAGCTTTGGAGAA+GGG | 0.553712 | 5.4:-17446986 | MS.gene83476:CDS |
| TCATTTACTTGTTCATCAGT+CGG | 0.558425 | 5.4:+17436499 | None:intergenic |
| CTTGAAAAGTGCCATCATGC+TGG | 0.558468 | 5.4:-17446893 | MS.gene83476:CDS |
| ACTGATGAACAAGTAAATGA+TGG | 0.558662 | 5.4:-17436496 | MS.gene83476:CDS |
| ACACATTTCTCAGAGCTCAA+TGG | 0.560141 | 5.4:-17441950 | MS.gene83476:intron |
| GTGGAGCAATAACAAGCTGC+AGG | 0.560375 | 5.4:-17443952 | MS.gene83476:CDS |
| GTTGAAGTTAGCATATATGC+TGG | 0.561545 | 5.4:+17447034 | None:intergenic |
| AAGAGATGTGCTGGTCATCA+TGG | 0.563733 | 5.4:-17447297 | MS.gene83476:CDS |
| CTTATCCAAATCACGAATCA+GGG | 0.563762 | 5.4:-17445289 | MS.gene83476:CDS |
| CATATACAAAGCTTTGGAGA+AGG | 0.565982 | 5.4:-17446987 | MS.gene83476:CDS |
| TGAGTAGACAAGACAGAGTG+TGG | 0.573006 | 5.4:+17437093 | None:intergenic |
| GCAAAGGAGCTAAGGGAGAG+AGG | 0.574170 | 5.4:-17444602 | MS.gene83476:CDS |
| AGGGAGAGAGGAATTTCAGC+TGG | 0.576672 | 5.4:-17444590 | MS.gene83476:CDS |
| TTAGGTTGCAAAGGAGCTAA+GGG | 0.577621 | 5.4:-17444609 | MS.gene83476:intron |
| CACTACTCTCAATAAGAACA+AGG | 0.584987 | 5.4:+17436440 | None:intergenic |
| CTTACAGTCAAAGCAACCAT+AGG | 0.590094 | 5.4:+17445708 | None:intergenic |
| AGGCACATTGCTGTAGTCAG+TGG | 0.592090 | 5.4:-17445794 | MS.gene83476:CDS |
| TGCTGCAAGTTCGAGTATTG+CGG | 0.593353 | 5.4:-17448378 | MS.gene83476:CDS |
| CTGGTCATCATGGCTGCAGG+TGG | 0.593654 | 5.4:-17447287 | MS.gene83476:CDS |
| AAGCTTTGGAGAAGGGCGAG+GGG | 0.602439 | 5.4:-17446979 | MS.gene83476:CDS |
| TGCATTCTCATGTGAGGTGA+AGG | 0.604384 | 5.4:-17440994 | MS.gene83476:CDS |
| AGTAATTGGAAAACTGAAGA+CGG | 0.605938 | 5.4:-17436575 | MS.gene83476:CDS |
| GCTGCAGGTCATAGTTGGTA+CGG | 0.606551 | 5.4:-17443937 | MS.gene83476:intron |
| ATTGAGTGGAAGAGATGTGC+TGG | 0.608585 | 5.4:-17447306 | MS.gene83476:CDS |
| CGTTAATTGAAAAGCAAGAG+AGG | 0.608856 | 5.4:-17448472 | MS.gene83476:CDS |
| ATCAATGGAAACATACTACC+AGG | 0.611015 | 5.4:-17442878 | MS.gene83476:intron |
| TCAGGCAACTGCTACTCAGA+GGG | 0.615092 | 5.4:-17445589 | MS.gene83476:intron |
| GTATTGCGGAGAATTGGAAT+GGG | 0.615248 | 5.4:-17448364 | MS.gene83476:CDS |
| ATGGGAATCAACAAACCAGA+TGG | 0.615528 | 5.4:-17443800 | MS.gene83476:intron |
| GAGAGAGACGACCAGCATGA+TGG | 0.616272 | 5.4:+17446882 | None:intergenic |
| GTCAACGACCACCTTACTGA+CGG | 0.619582 | 5.4:+17445337 | None:intergenic |
| TGGGAGTATTCCTCCATGAG+TGG | 0.623965 | 5.4:+17437113 | None:intergenic |
| AAGTATAATAGGCATTCAGA+AGG | 0.625558 | 5.4:+17442433 | None:intergenic |
| GAAGCTTGAGATTTCTACAG+AGG | 0.633669 | 5.4:-17437226 | MS.gene83476:CDS |
| CATATATGCTGGGATTCCCA+AGG | 0.636281 | 5.4:+17447045 | None:intergenic |
| CTCATGTGAGGTGAAGGAAG+TGG | 0.642270 | 5.4:-17440988 | MS.gene83476:CDS |
| GAACTTTCTTCCACTCATGG+AGG | 0.642311 | 5.4:-17437123 | MS.gene83476:CDS |
| AGCTTTGGAGAAGGGCGAGG+GGG | 0.643222 | 5.4:-17446978 | MS.gene83476:CDS |
| GAGTAGACAAGACAGAGTGT+GGG | 0.645165 | 5.4:+17437094 | None:intergenic |
| TGCCTATTATACTTCAGACC+TGG | 0.648906 | 5.4:-17442424 | MS.gene83476:CDS |
| AAAGTCATCCGTCAGTAAGG+TGG | 0.649992 | 5.4:-17445345 | MS.gene83476:CDS |
| GTCAGGCAACTGCTACTCAG+AGG | 0.652846 | 5.4:-17445590 | MS.gene83476:intron |
| ATATTCAGGCAAGCGATCAG+AGG | 0.654836 | 5.4:-17440750 | MS.gene83476:CDS |
| GAGCCATTACAGGAGTGCAA+TGG | 0.655138 | 5.4:-17441126 | MS.gene83476:intron |
| AGGTTCTGACTTAAAACGAG+AGG | 0.658026 | 5.4:-17438585 | MS.gene83476:CDS |
| TCTTGAATTAGTGAAAGCAA+AGG | 0.660230 | 5.4:+17447200 | None:intergenic |
| GCTCCAAGCCAGATGACCCG+AGG | 0.687823 | 5.4:+17437169 | None:intergenic |
| GCACATTGCTGTAGTCAGTG+GGG | 0.716168 | 5.4:-17445792 | MS.gene83476:CDS |
| CATATGTGCATTCTCATGTG+AGG | 0.730818 | 5.4:-17441000 | MS.gene83476:CDS |
| ACTGAGTCTTGAGAATACCA+AGG | 0.736426 | 5.4:+17445742 | None:intergenic |
| CAAATGCTTACATTACTGTG+GGG | 0.745077 | 5.4:-17437579 | MS.gene83476:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 17436426 | 17448672 | 17436426 | ID=MS.gene83476 |
| chr5.4 | mRNA | 17436426 | 17448672 | 17436426 | ID=MS.gene83476.t1;Parent=MS.gene83476 |
| chr5.4 | exon | 17448609 | 17448672 | 17448609 | ID=MS.gene83476.t1.exon1;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17448609 | 17448672 | 17448609 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17448283 | 17448506 | 17448283 | ID=MS.gene83476.t1.exon2;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17448283 | 17448506 | 17448283 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17447196 | 17447342 | 17447196 | ID=MS.gene83476.t1.exon3;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17447196 | 17447342 | 17447196 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17446868 | 17447083 | 17446868 | ID=MS.gene83476.t1.exon4;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17446868 | 17447083 | 17446868 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17445713 | 17445817 | 17445713 | ID=MS.gene83476.t1.exon5;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17445713 | 17445817 | 17445713 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17445503 | 17445607 | 17445503 | ID=MS.gene83476.t1.exon6;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17445503 | 17445607 | 17445503 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17445253 | 17445375 | 17445253 | ID=MS.gene83476.t1.exon7;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17445253 | 17445375 | 17445253 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17444539 | 17444627 | 17444539 | ID=MS.gene83476.t1.exon8;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17444539 | 17444627 | 17444539 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17443938 | 17443974 | 17443938 | ID=MS.gene83476.t1.exon9;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17443938 | 17443974 | 17443938 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17443801 | 17443834 | 17443801 | ID=MS.gene83476.t1.exon10;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17443801 | 17443834 | 17443801 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17442879 | 17442931 | 17442879 | ID=MS.gene83476.t1.exon11;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17442879 | 17442931 | 17442879 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17442408 | 17442485 | 17442408 | ID=MS.gene83476.t1.exon12;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17442408 | 17442485 | 17442408 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17441894 | 17441959 | 17441894 | ID=MS.gene83476.t1.exon13;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17441894 | 17441959 | 17441894 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17441127 | 17441193 | 17441127 | ID=MS.gene83476.t1.exon14;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17441127 | 17441193 | 17441127 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17440979 | 17441032 | 17440979 | ID=MS.gene83476.t1.exon15;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17440979 | 17441032 | 17440979 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17440698 | 17440805 | 17440698 | ID=MS.gene83476.t1.exon16;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17440698 | 17440805 | 17440698 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17438541 | 17438605 | 17438541 | ID=MS.gene83476.t1.exon17;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17438541 | 17438605 | 17438541 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17437554 | 17437632 | 17437554 | ID=MS.gene83476.t1.exon18;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17437554 | 17437632 | 17437554 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17437047 | 17437261 | 17437047 | ID=MS.gene83476.t1.exon19;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17437047 | 17437261 | 17437047 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| chr5.4 | exon | 17436426 | 17436605 | 17436426 | ID=MS.gene83476.t1.exon20;Parent=MS.gene83476.t1 |
| chr5.4 | CDS | 17436426 | 17436605 | 17436426 | ID=cds.MS.gene83476.t1;Parent=MS.gene83476.t1 |
| Gene Sequence |
| Protein sequence |
