Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72812.t1 | RHN54641.1 | 97.9 | 702 | 15 | 0 | 1 | 702 | 1 | 702 | 0.00E+00 | 1283.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72812.t1 | Q9FT73 | 73.8 | 665 | 164 | 2 | 43 | 701 | 43 | 703 | 6.5e-285 | 981.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72812.t1 | A0A396HQ63 | 97.9 | 702 | 15 | 0 | 1 | 702 | 1 | 702 | 0.0e+00 | 1283.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene72812 | 0.813198 | 2.89E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene72812 | 0.814615 | 1.41E-51 | -1.69E-46 |
| MS.gene049501 | MS.gene72812 | 0.808308 | 3.30E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene72812 | 0.828685 | 7.83E-55 | -1.69E-46 |
| MS.gene049979 | MS.gene72812 | 0.808586 | 2.88E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene72812 | 0.812303 | 4.54E-51 | -1.69E-46 |
| MS.gene050137 | MS.gene72812 | 0.802994 | 4.31E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene72812 | 0.807211 | 5.65E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene72812 | 0.804577 | 2.02E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene72812 | 0.810473 | 1.13E-50 | -1.69E-46 |
| MS.gene050438 | MS.gene72812 | 0.821589 | 3.73E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene72812 | 0.832892 | 7.29E-56 | -1.69E-46 |
| MS.gene050787 | MS.gene72812 | 0.82606 | 3.34E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene72812 | 0.841699 | 4.06E-58 | -1.69E-46 |
| MS.gene050815 | MS.gene72812 | 0.819418 | 1.17E-52 | -1.69E-46 |
| MS.gene050959 | MS.gene72812 | 0.848977 | 4.35E-60 | -1.69E-46 |
| MS.gene051180 | MS.gene72812 | 0.812653 | 3.80E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene72812 | 0.80096 | 1.13E-48 | -1.69E-46 |
| MS.gene051265 | MS.gene72812 | 0.824092 | 9.73E-54 | -1.69E-46 |
| MS.gene052020 | MS.gene72812 | 0.842319 | 2.78E-58 | -1.69E-46 |
| MS.gene052323 | MS.gene72812 | 0.809531 | 1.81E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene72812 | 0.810679 | 1.02E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene72812 | 0.807523 | 4.85E-50 | -1.69E-46 |
| MS.gene052613 | MS.gene72812 | 0.823749 | 1.17E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene72812 | 0.842461 | 2.55E-58 | -1.69E-46 |
| MS.gene052856 | MS.gene72812 | 0.803814 | 2.91E-49 | -1.69E-46 |
| MS.gene052858 | MS.gene72812 | 0.809646 | 1.71E-50 | -1.69E-46 |
| MS.gene052860 | MS.gene72812 | 0.814411 | 1.56E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene72812 | 0.819973 | 8.76E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene72812 | 0.800779 | 1.23E-48 | -1.69E-46 |
| MS.gene053074 | MS.gene72812 | 0.811872 | 5.63E-51 | -1.69E-46 |
| MS.gene053184 | MS.gene72812 | 0.804929 | 1.71E-49 | -1.69E-46 |
| MS.gene053221 | MS.gene72812 | 0.824489 | 7.85E-54 | -1.69E-46 |
| MS.gene054184 | MS.gene72812 | 0.802756 | 4.82E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene72812 | 0.813912 | 2.01E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene72812 | 0.816424 | 5.57E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene72812 | 0.801876 | 7.32E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene72812 | 0.833361 | 5.57E-56 | -1.69E-46 |
| MS.gene054942 | MS.gene72812 | 0.802081 | 6.65E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene72812 | 0.80514 | 1.54E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene72812 | 0.822606 | 2.17E-53 | -1.69E-46 |
| MS.gene055358 | MS.gene72812 | 0.827527 | 1.49E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene72812 | 0.805929 | 1.05E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene72812 | 0.818952 | 1.50E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene72812 | 0.805902 | 1.07E-49 | -1.69E-46 |
| MS.gene055608 | MS.gene72812 | 0.818551 | 1.85E-52 | -1.69E-46 |
| MS.gene055816 | MS.gene72812 | -0.812104 | 5.01E-51 | -1.69E-46 |
| MS.gene05604 | MS.gene72812 | 0.802468 | 5.53E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene72812 | -0.801681 | 8.03E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene72812 | 0.801895 | 7.26E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene72812 | 0.811123 | 8.19E-51 | -1.69E-46 |
| MS.gene056643 | MS.gene72812 | 0.816863 | 4.44E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene72812 | 0.829345 | 5.42E-55 | -1.69E-46 |
| MS.gene058084 | MS.gene72812 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene72812 | 0.835885 | 1.29E-56 | -1.69E-46 |
| MS.gene059725 | MS.gene72812 | 0.827829 | 1.26E-54 | -1.69E-46 |
| MS.gene059937 | MS.gene72812 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene060137 | MS.gene72812 | -0.805406 | 1.36E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene72812 | 0.808809 | 2.58E-50 | -1.69E-46 |
| MS.gene060619 | MS.gene72812 | 0.806266 | 8.94E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene72812 | 0.84573 | 3.39E-59 | -1.69E-46 |
| MS.gene060998 | MS.gene72812 | 0.805127 | 1.55E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene87672 | MS.gene72812 | PPI |
| MS.gene85235 | MS.gene72812 | PPI |
| MS.gene07826 | MS.gene72812 | PPI |
| MS.gene051963 | MS.gene72812 | PPI |
| MS.gene011242 | MS.gene72812 | PPI |
| MS.gene37366 | MS.gene72812 | PPI |
| MS.gene025141 | MS.gene72812 | PPI |
| MS.gene27517 | MS.gene72812 | PPI |
| MS.gene052695 | MS.gene72812 | PPI |
| MS.gene056998 | MS.gene72812 | PPI |
| MS.gene032228 | MS.gene72812 | PPI |
| MS.gene92776 | MS.gene72812 | PPI |
| MS.gene036929 | MS.gene72812 | PPI |
| MS.gene85610 | MS.gene72812 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72812.t1 | MTR_5g026590 | 93.457 | 703 | 27 | 2 | 1 | 702 | 1 | 685 | 0.0 | 1345 |
| MS.gene72812.t1 | MTR_2g006440 | 37.391 | 690 | 358 | 16 | 71 | 690 | 390 | 1075 | 3.92e-133 | 421 |
| MS.gene72812.t1 | MTR_1g083840 | 41.149 | 435 | 232 | 9 | 61 | 475 | 168 | 598 | 1.48e-106 | 337 |
| MS.gene72812.t1 | MTR_2g042440 | 40.268 | 447 | 211 | 8 | 85 | 503 | 38 | 456 | 9.95e-105 | 335 |
| MS.gene72812.t1 | MTR_2g042440 | 40.268 | 447 | 211 | 8 | 85 | 503 | 38 | 456 | 1.15e-104 | 335 |
| MS.gene72812.t1 | MTR_3g063040 | 41.822 | 428 | 220 | 12 | 83 | 498 | 10 | 420 | 1.73e-93 | 310 |
| MS.gene72812.t1 | MTR_8g080160 | 36.982 | 338 | 197 | 7 | 84 | 417 | 271 | 596 | 2.87e-51 | 192 |
| MS.gene72812.t1 | MTR_1g083250 | 39.583 | 240 | 125 | 6 | 61 | 289 | 136 | 366 | 5.23e-43 | 165 |
| MS.gene72812.t1 | MTR_1g083250 | 41.346 | 104 | 61 | 0 | 317 | 420 | 510 | 613 | 2.71e-20 | 95.9 |
| MS.gene72812.t1 | MTR_1g083250 | 39.583 | 240 | 125 | 6 | 61 | 289 | 136 | 366 | 2.69e-42 | 164 |
| MS.gene72812.t1 | MTR_1g083250 | 36.364 | 165 | 97 | 4 | 317 | 475 | 510 | 672 | 3.68e-25 | 111 |
| MS.gene72812.t1 | MTR_1g083250 | 39.583 | 240 | 125 | 6 | 61 | 289 | 136 | 366 | 3.91e-42 | 164 |
| MS.gene72812.t1 | MTR_1g083250 | 36.364 | 165 | 97 | 4 | 317 | 475 | 510 | 672 | 4.13e-25 | 111 |
| MS.gene72812.t1 | MTR_1g026830 | 21.495 | 428 | 264 | 14 | 86 | 479 | 165 | 554 | 3.27e-13 | 73.2 |
| MS.gene72812.t1 | MTR_2g100230 | 23.894 | 339 | 211 | 10 | 102 | 417 | 130 | 444 | 6.87e-13 | 72.0 |
| MS.gene72812.t1 | MTR_4g105100 | 23.512 | 336 | 216 | 12 | 102 | 417 | 236 | 550 | 6.93e-12 | 69.3 |
| MS.gene72812.t1 | MTR_1g095810 | 21.875 | 352 | 222 | 10 | 86 | 408 | 163 | 490 | 6.65e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72812.t1 | AT1G31360 | 72.702 | 707 | 183 | 2 | 1 | 701 | 1 | 703 | 0.0 | 1089 |
| MS.gene72812.t1 | AT1G31360 | 72.702 | 707 | 183 | 2 | 1 | 701 | 35 | 737 | 0.0 | 1088 |
| MS.gene72812.t1 | AT1G31360 | 75.272 | 643 | 155 | 1 | 1 | 643 | 1 | 639 | 0.0 | 1032 |
| MS.gene72812.t1 | AT1G31360 | 74.399 | 582 | 139 | 2 | 126 | 701 | 1 | 578 | 0.0 | 914 |
| MS.gene72812.t1 | AT1G31360 | 76.881 | 545 | 126 | 0 | 1 | 545 | 1 | 545 | 0.0 | 903 |
| MS.gene72812.t1 | AT1G31360 | 76.514 | 545 | 120 | 2 | 1 | 545 | 1 | 537 | 0.0 | 885 |
| MS.gene72812.t1 | AT1G31360 | 78.585 | 523 | 112 | 0 | 1 | 523 | 1 | 523 | 0.0 | 884 |
| MS.gene72812.t1 | AT1G31360 | 78.585 | 523 | 112 | 0 | 1 | 523 | 1 | 523 | 0.0 | 884 |
| MS.gene72812.t1 | AT1G10930 | 37.031 | 667 | 336 | 14 | 71 | 664 | 435 | 1090 | 3.70e-129 | 411 |
| MS.gene72812.t1 | AT4G35740 | 42.298 | 409 | 217 | 7 | 85 | 481 | 32 | 433 | 6.56e-105 | 336 |
| MS.gene72812.t1 | AT3G05740 | 42.607 | 399 | 212 | 6 | 78 | 463 | 107 | 501 | 1.78e-102 | 323 |
| MS.gene72812.t1 | AT3G05740 | 42.607 | 399 | 212 | 6 | 78 | 463 | 192 | 586 | 1.00e-101 | 324 |
| MS.gene72812.t1 | AT4G35740 | 40.634 | 347 | 187 | 7 | 147 | 481 | 1 | 340 | 1.08e-77 | 261 |
| MS.gene72812.t1 | AT1G27880 | 34.456 | 386 | 229 | 9 | 43 | 417 | 218 | 590 | 3.81e-52 | 194 |
| MS.gene72812.t1 | AT1G27880 | 34.456 | 386 | 229 | 9 | 43 | 417 | 218 | 590 | 7.92e-52 | 194 |
| MS.gene72812.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 2.90e-43 | 168 |
| MS.gene72812.t1 | AT5G27680 | 30.688 | 189 | 91 | 6 | 317 | 497 | 501 | 657 | 6.37e-14 | 75.9 |
| MS.gene72812.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.06e-43 | 168 |
| MS.gene72812.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.33e-28 | 122 |
| MS.gene72812.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.06e-43 | 168 |
| MS.gene72812.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.33e-28 | 122 |
| MS.gene72812.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.06e-43 | 168 |
| MS.gene72812.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.33e-28 | 122 |
| MS.gene72812.t1 | AT5G27680 | 36.299 | 281 | 152 | 7 | 19 | 289 | 107 | 370 | 3.06e-43 | 168 |
| MS.gene72812.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 501 | 684 | 1.33e-28 | 122 |
| MS.gene72812.t1 | AT5G27680 | 40.088 | 227 | 118 | 5 | 73 | 289 | 22 | 240 | 6.59e-43 | 166 |
| MS.gene72812.t1 | AT5G27680 | 38.095 | 189 | 104 | 5 | 317 | 497 | 371 | 554 | 1.23e-28 | 122 |
| MS.gene72812.t1 | AT5G63120 | 22.414 | 348 | 205 | 10 | 102 | 417 | 200 | 514 | 1.87e-12 | 70.9 |
| MS.gene72812.t1 | AT1G55150 | 22.380 | 353 | 199 | 11 | 102 | 417 | 134 | 448 | 2.12e-11 | 67.4 |
| MS.gene72812.t1 | AT1G55150 | 22.380 | 353 | 199 | 11 | 102 | 417 | 159 | 473 | 2.12e-11 | 67.4 |
Find 127 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTGAACTAAATTCGATTT+TGG | 0.157671 | 5.1:-17258302 | MS.gene72812:CDS |
| TTCTTTGTTGCAAGATATTC+AGG | 0.234107 | 5.1:-17248159 | MS.gene72812:CDS |
| ACATCTCTACAAGATCATTT+TGG | 0.244904 | 5.1:+17253527 | None:intergenic |
| ACAATCCCTGATTCGTGATT+TGG | 0.255388 | 5.1:+17253238 | None:intergenic |
| TATACCATGTAAAATAAATT+TGG | 0.262283 | 5.1:+17253460 | None:intergenic |
| CTTGCCTCTTCCAGTGAGTT+TGG | 0.267298 | 5.1:+17244412 | None:intergenic |
| GTGCAGTTGGAGAAAGTAAT+TGG | 0.274668 | 5.1:-17243706 | MS.gene72812:intron |
| TTTACATTTCAGGTAGCTTT+TGG | 0.276488 | 5.1:-17251065 | MS.gene72812:intron |
| ACTTCGACGGCATTGTCTTT+TGG | 0.284094 | 5.1:+17248597 | None:intergenic |
| CCAAAGCACGCTCCAGTAAA+AGG | 0.284767 | 5.1:+17245916 | None:intergenic |
| ATTCTCAAGCTGATGATCTT+AGG | 0.305060 | 5.1:-17258195 | MS.gene72812:CDS |
| AATAAGAACAAGGTTCTTCT+TGG | 0.307971 | 5.1:+17243567 | None:intergenic |
| AAAGCAACCATAGGAACATT+AGG | 0.308648 | 5.1:+17253679 | None:intergenic |
| AAAACAAAACAGAAGGAAAT+AGG | 0.313249 | 5.1:-17248092 | MS.gene72812:intron |
| ATGGTCTTCTATGAAAATTC+AGG | 0.314044 | 5.1:-17249356 | MS.gene72812:CDS |
| CTCAGCAAAATGCCTGAAAA+AGG | 0.319842 | 5.1:+17248573 | None:intergenic |
| TCTTATCCAAATCACGAATC+AGG | 0.333951 | 5.1:-17253244 | MS.gene72812:CDS |
| CACATCTCTTCCACTCAATA+TGG | 0.350170 | 5.1:+17257177 | None:intergenic |
| CACCAGGTCTGAAGTATAAT+AGG | 0.356483 | 5.1:+17250001 | None:intergenic |
| AAACGCAGCCTCGGGTCATC+TGG | 0.375770 | 5.1:-17244547 | MS.gene72812:CDS |
| GTTCGAGTGTTGTGGAGAAT+TGG | 0.383508 | 5.1:-17258237 | MS.gene72812:CDS |
| GTGTGGTTGCTTCAGGTAAT+GGG | 0.386890 | 5.1:-17256947 | MS.gene72812:intron |
| TGCAGGGCATGCAAAATTGA+TGG | 0.387317 | 5.1:-17248183 | MS.gene72812:intron |
| TTCATCAGTCGGCTCAAAAT+CGG | 0.397305 | 5.1:+17243627 | None:intergenic |
| TGACTTAAAACGAGAAGAAA+TGG | 0.404058 | 5.1:-17245955 | MS.gene72812:CDS |
| GTGTAAAGAATCTGAAAATC+AGG | 0.408828 | 5.1:-17258275 | MS.gene72812:CDS |
| GAAGTTCATATACAAAGCTT+TGG | 0.411764 | 5.1:-17256864 | MS.gene72812:CDS |
| CAGCCTCGGGTCATCTGGCT+TGG | 0.413431 | 5.1:-17244542 | MS.gene72812:CDS |
| ATTGGAACGGGACGTTTGAA+TGG | 0.415913 | 5.1:-17258219 | MS.gene72812:CDS |
| GGTAATGGGTTTGACTGCCT+TGG | 0.427589 | 5.1:-17256933 | MS.gene72812:CDS |
| ATACCAAGGTTCTTGTAATC+AGG | 0.433950 | 5.1:+17253718 | None:intergenic |
| CTCTAGGAAGGAATGTGAAC+AGG | 0.437358 | 5.1:-17253206 | MS.gene72812:intron |
| TGCAAAATTTGCTTCGCCAA+TGG | 0.440678 | 5.1:+17244926 | None:intergenic |
| GTAATGGGTTTGACTGCCTT+GGG | 0.449141 | 5.1:-17256932 | MS.gene72812:CDS |
| TCAGTTCCCTAATGTTCCTA+TGG | 0.451520 | 5.1:-17253686 | MS.gene72812:CDS |
| GCTTTCGCTAATTCAAGATC+AGG | 0.460053 | 5.1:-17257062 | MS.gene72812:intron |
| AATTTGTCAGCACAGTCAAT+AGG | 0.468637 | 5.1:-17253483 | MS.gene72812:CDS |
| GTCATCATGGCTGCAGGTGG+TGG | 0.469281 | 5.1:-17257151 | MS.gene72812:CDS |
| AAGCAACCATAGGAACATTA+GGG | 0.471975 | 5.1:+17253680 | None:intergenic |
| AAATTGAAGACGGAAAAGTA+TGG | 0.475427 | 5.1:-17243682 | MS.gene72812:CDS |
| CTTCCTGCTGTTCTTCGCAA+TGG | 0.476512 | 5.1:-17257109 | MS.gene72812:CDS |
| AAAGAACTTTCTTCCACTCA+TGG | 0.477688 | 5.1:-17244496 | MS.gene72812:CDS |
| GTACCATTGCACTCCTGTAA+TGG | 0.481444 | 5.1:+17248550 | None:intergenic |
| GCTAACTTCAACAACAAGTA+AGG | 0.482808 | 5.1:-17256894 | MS.gene72812:CDS |
| TGGTCATTATCATGCAGATA+TGG | 0.487893 | 5.1:-17251813 | MS.gene72812:CDS |
| TTTAGGTTGCAAAGGAGCTA+AGG | 0.491708 | 5.1:-17251853 | MS.gene72812:intron |
| AGTGGTCGAGCTGGTCGAGA+TGG | 0.494642 | 5.1:-17250039 | MS.gene72812:CDS |
| TCACAGCTTGAGTAAATCAA+TGG | 0.495744 | 5.1:-17250419 | MS.gene72812:CDS |
| TTGGAACGGGACGTTTGAAT+GGG | 0.503155 | 5.1:-17258218 | MS.gene72812:CDS |
| AAAACCAAACTCACTGGAAG+AGG | 0.507726 | 5.1:-17244416 | MS.gene72812:intron |
| TTACATTACTGTGGGGCCAT+TGG | 0.508759 | 5.1:-17244942 | MS.gene72812:CDS |
| GCTGAAATCGAAGATGTTCA+AGG | 0.509003 | 5.1:-17258474 | MS.gene72812:intron |
| CGACCTGATTACAAGAACCT+TGG | 0.510980 | 5.1:-17253721 | MS.gene72812:CDS |
| GGAAAAGTCATCCGTCAGTA+AGG | 0.511037 | 5.1:-17253302 | MS.gene72812:CDS |
| AACATTACTTTAGGTTGCAA+AGG | 0.512532 | 5.1:-17251861 | MS.gene72812:intron |
| TCAGACCTGGTGATGTCCCC+CGG | 0.513480 | 5.1:-17249990 | MS.gene72812:CDS |
| CAAAGCTTTGGAGAAGGGCG+AGG | 0.515095 | 5.1:-17256852 | MS.gene72812:CDS |
| TGACATAGTACGATATTGTC+AGG | 0.518563 | 5.1:-17249318 | MS.gene72812:intron |
| TAAGATTGAAACGCAGCCTC+GGG | 0.520222 | 5.1:-17244555 | MS.gene72812:CDS |
| TTGATTTACTCAAGCTGTGA+TGG | 0.520373 | 5.1:+17250422 | None:intergenic |
| ATGATGGTAGAGCTGCAAAA+AGG | 0.524588 | 5.1:-17243597 | MS.gene72812:CDS |
| TGCGAAGAACAGCAGGAAGC+TGG | 0.525627 | 5.1:+17257113 | None:intergenic |
| ACAAATGCTTACATTACTGT+GGG | 0.528063 | 5.1:-17244950 | MS.gene72812:CDS |
| TGTTTATTGCTTCTCTAGGA+AGG | 0.528457 | 5.1:-17253218 | MS.gene72812:CDS |
| CAACAGGAAAGTGGTCGAGC+TGG | 0.528486 | 5.1:-17250048 | MS.gene72812:intron |
| GGCACATTGCTGTAGTCAGT+GGG | 0.529118 | 5.1:-17253755 | MS.gene72812:CDS |
| ACTCCATTGCGAAGAACAGC+AGG | 0.530357 | 5.1:+17257106 | None:intergenic |
| GTAAGATTGAAACGCAGCCT+CGG | 0.535718 | 5.1:-17244556 | MS.gene72812:CDS |
| AAAGCTTTGGAGAAGGGCGA+GGG | 0.537834 | 5.1:-17256851 | MS.gene72812:CDS |
| TACAAATGCTTACATTACTG+TGG | 0.538641 | 5.1:-17244951 | MS.gene72812:CDS |
| AACAAGCTGCAGGTCATAGT+TGG | 0.538884 | 5.1:-17251183 | MS.gene72812:CDS |
| GTGTTGTGGAGAATTGGAAC+GGG | 0.540609 | 5.1:-17258231 | MS.gene72812:CDS |
| GTGGTCGAGCTGGTCGAGAT+GGG | 0.541598 | 5.1:-17250038 | MS.gene72812:CDS |
| GGATTGTTTATTGCTTCTCT+AGG | 0.542756 | 5.1:-17253222 | MS.gene72812:CDS |
| ATTATTAATGCCATATTGAG+TGG | 0.542763 | 5.1:-17257187 | MS.gene72812:CDS |
| GTGCTGGTCATCATGGCTGC+AGG | 0.544531 | 5.1:-17257157 | MS.gene72812:CDS |
| TTGAAGTTAGCATATATGCT+GGG | 0.553613 | 5.1:+17256906 | None:intergenic |
| ATATACAAAGCTTTGGAGAA+GGG | 0.553712 | 5.1:-17256857 | MS.gene72812:CDS |
| TCATTTACTTGTTCATCAGT+CGG | 0.558425 | 5.1:+17243616 | None:intergenic |
| CTTGAAAAGTGCCATCATGC+TGG | 0.558468 | 5.1:-17256764 | MS.gene72812:CDS |
| ACTGATGAACAAGTAAATGA+TGG | 0.558662 | 5.1:-17243613 | MS.gene72812:CDS |
| ACACATTTCTCAGAGCTCAA+TGG | 0.560141 | 5.1:-17249375 | MS.gene72812:intron |
| GTGGAGCAATAACAAGCTGC+AGG | 0.560375 | 5.1:-17251193 | MS.gene72812:CDS |
| GTTGAAGTTAGCATATATGC+TGG | 0.561545 | 5.1:+17256905 | None:intergenic |
| AAGAGATGTGCTGGTCATCA+TGG | 0.563733 | 5.1:-17257164 | MS.gene72812:CDS |
| CTTATCCAAATCACGAATCA+GGG | 0.563762 | 5.1:-17253243 | MS.gene72812:CDS |
| CATATACAAAGCTTTGGAGA+AGG | 0.565982 | 5.1:-17256858 | MS.gene72812:CDS |
| TGAGTAGACAAGACAGAGTG+TGG | 0.573006 | 5.1:+17244463 | None:intergenic |
| GCAAAGGAGCTAAGGGAGAG+AGG | 0.574170 | 5.1:-17251845 | MS.gene72812:CDS |
| AGGGAGAGAGGAATTTCAGC+TGG | 0.576672 | 5.1:-17251833 | MS.gene72812:CDS |
| TTAGGTTGCAAAGGAGCTAA+GGG | 0.577621 | 5.1:-17251852 | MS.gene72812:intron |
| AGTAATTGGAAAATTGAAGA+CGG | 0.579666 | 5.1:-17243692 | MS.gene72812:CDS |
| TGCTGCAAGTTCGAGTGTTG+TGG | 0.580841 | 5.1:-17258245 | MS.gene72812:CDS |
| CACTACTCTCAATAAGAACA+AGG | 0.584987 | 5.1:+17243557 | None:intergenic |
| CTTACAGTCAAAGCAACCAT+AGG | 0.590094 | 5.1:+17253670 | None:intergenic |
| AGGCACATTGCTGTAGTCAG+TGG | 0.592090 | 5.1:-17253756 | MS.gene72812:CDS |
| CTGGTCATCATGGCTGCAGG+TGG | 0.593654 | 5.1:-17257154 | MS.gene72812:CDS |
| AGTGTTGTGGAGAATTGGAA+CGG | 0.602354 | 5.1:-17258232 | MS.gene72812:CDS |
| AAGCTTTGGAGAAGGGCGAG+GGG | 0.602439 | 5.1:-17256850 | MS.gene72812:CDS |
| TGCATTCTCATGTGAGGTGA+AGG | 0.604384 | 5.1:-17248389 | MS.gene72812:CDS |
| GCTGCAGGTCATAGTTGGTA+CGG | 0.606551 | 5.1:-17251178 | MS.gene72812:intron |
| ATTGAGTGGAAGAGATGTGC+TGG | 0.608585 | 5.1:-17257173 | MS.gene72812:CDS |
| CGTTAATTGAAAAGCAAGAG+AGG | 0.608856 | 5.1:-17258339 | MS.gene72812:CDS |
| ATCAATGGAAACATACTACC+AGG | 0.611015 | 5.1:-17250404 | MS.gene72812:intron |
| TCAGGCAACTGCTACTCAGA+GGG | 0.615092 | 5.1:-17253551 | MS.gene72812:intron |
| ATGGGAATCAACAAACCAGA+TGG | 0.615528 | 5.1:-17251041 | MS.gene72812:intron |
| GAGAGAGACGACCAGCATGA+TGG | 0.616272 | 5.1:+17256753 | None:intergenic |
| GTCAACGACCACCTTACTGA+CGG | 0.619582 | 5.1:+17253291 | None:intergenic |
| TGGGAGTATTCCTCCATGAG+TGG | 0.623965 | 5.1:+17244483 | None:intergenic |
| AAGTATAATAGGCATTCAGA+AGG | 0.625558 | 5.1:+17250012 | None:intergenic |
| GAAGCTTGAGATTTCTACAG+AGG | 0.633669 | 5.1:-17244596 | MS.gene72812:CDS |
| CATATATGCTGGGATTCCCA+AGG | 0.636281 | 5.1:+17256916 | None:intergenic |
| CTCATGTGAGGTGAAGGAAG+TGG | 0.642270 | 5.1:-17248383 | MS.gene72812:CDS |
| GAACTTTCTTCCACTCATGG+AGG | 0.642311 | 5.1:-17244493 | MS.gene72812:CDS |
| AGCTTTGGAGAAGGGCGAGG+GGG | 0.643222 | 5.1:-17256849 | MS.gene72812:CDS |
| GAGTAGACAAGACAGAGTGT+GGG | 0.645165 | 5.1:+17244464 | None:intergenic |
| TGCCTATTATACTTCAGACC+TGG | 0.648906 | 5.1:-17250003 | MS.gene72812:CDS |
| AAAGTCATCCGTCAGTAAGG+TGG | 0.649992 | 5.1:-17253299 | MS.gene72812:CDS |
| GTCAGGCAACTGCTACTCAG+AGG | 0.652846 | 5.1:-17253552 | MS.gene72812:intron |
| ATATTCAGGCAAGCGATCAG+AGG | 0.654836 | 5.1:-17248145 | MS.gene72812:CDS |
| GAGCCATTACAGGAGTGCAA+TGG | 0.655138 | 5.1:-17248553 | MS.gene72812:intron |
| TCTTGAATTAGCGAAAGCAA+AGG | 0.661736 | 5.1:+17257067 | None:intergenic |
| GCTCCAAGCCAGATGACCCG+AGG | 0.687823 | 5.1:+17244539 | None:intergenic |
| GCACATTGCTGTAGTCAGTG+GGG | 0.716168 | 5.1:-17253754 | MS.gene72812:CDS |
| CATATGTGCATTCTCATGTG+AGG | 0.730818 | 5.1:-17248395 | MS.gene72812:CDS |
| ACTGAGTCTTGAGAATACCA+AGG | 0.736426 | 5.1:+17253704 | None:intergenic |
| CAAATGCTTACATTACTGTG+GGG | 0.745077 | 5.1:-17244949 | MS.gene72812:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 17243543 | 17258538 | 17243543 | ID=MS.gene72812 |
| chr5.1 | mRNA | 17243543 | 17258538 | 17243543 | ID=MS.gene72812.t1;Parent=MS.gene72812 |
| chr5.1 | exon | 17258475 | 17258538 | 17258475 | ID=MS.gene72812.t1.exon1;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17258475 | 17258538 | 17258475 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17258150 | 17258373 | 17258150 | ID=MS.gene72812.t1.exon2;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17258150 | 17258373 | 17258150 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17257063 | 17257209 | 17257063 | ID=MS.gene72812.t1.exon3;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17257063 | 17257209 | 17257063 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17256739 | 17256954 | 17256739 | ID=MS.gene72812.t1.exon4;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17256739 | 17256954 | 17256739 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17253675 | 17253779 | 17253675 | ID=MS.gene72812.t1.exon5;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17253675 | 17253779 | 17253675 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17253465 | 17253569 | 17253465 | ID=MS.gene72812.t1.exon6;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17253465 | 17253569 | 17253465 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17253207 | 17253329 | 17253207 | ID=MS.gene72812.t1.exon7;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17253207 | 17253329 | 17253207 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17251782 | 17251870 | 17251782 | ID=MS.gene72812.t1.exon8;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17251782 | 17251870 | 17251782 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17251179 | 17251215 | 17251179 | ID=MS.gene72812.t1.exon9;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17251179 | 17251215 | 17251179 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17251042 | 17251075 | 17251042 | ID=MS.gene72812.t1.exon10;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17251042 | 17251075 | 17251042 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17250405 | 17250457 | 17250405 | ID=MS.gene72812.t1.exon11;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17250405 | 17250457 | 17250405 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17249987 | 17250064 | 17249987 | ID=MS.gene72812.t1.exon12;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17249987 | 17250064 | 17249987 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17249319 | 17249384 | 17249319 | ID=MS.gene72812.t1.exon13;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17249319 | 17249384 | 17249319 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17248554 | 17248620 | 17248554 | ID=MS.gene72812.t1.exon14;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17248554 | 17248620 | 17248554 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17248374 | 17248427 | 17248374 | ID=MS.gene72812.t1.exon15;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17248374 | 17248427 | 17248374 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17248093 | 17248200 | 17248093 | ID=MS.gene72812.t1.exon16;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17248093 | 17248200 | 17248093 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17245917 | 17245981 | 17245917 | ID=MS.gene72812.t1.exon17;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17245917 | 17245981 | 17245917 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17244924 | 17245002 | 17244924 | ID=MS.gene72812.t1.exon18;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17244924 | 17245002 | 17244924 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17244417 | 17244631 | 17244417 | ID=MS.gene72812.t1.exon19;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17244417 | 17244631 | 17244417 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| chr5.1 | exon | 17243543 | 17243722 | 17243543 | ID=MS.gene72812.t1.exon20;Parent=MS.gene72812.t1 |
| chr5.1 | CDS | 17243543 | 17243722 | 17243543 | ID=cds.MS.gene72812.t1;Parent=MS.gene72812.t1 |
| Gene Sequence |
| Protein sequence |
