AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043348

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043348.t1 MTR_2g089670 89.958 478 46 1 1 476 6 483 0.0 846
MS.gene043348.t1 MTR_2g089765 71.926 488 112 7 5 476 10 488 0.0 706
MS.gene043348.t1 MTR_8g012750 65.281 481 141 14 15 474 1 476 0.0 582
MS.gene043348.t1 MTR_2g089665 61.072 429 72 3 17 445 2 335 5.26e-178 502
MS.gene043348.t1 MTR_7g015860 44.142 478 225 11 17 472 5 462 8.46e-125 372
MS.gene043348.t1 MTR_3g028210 37.527 469 272 10 17 473 8 467 2.28e-94 293
MS.gene043348.t1 MTR_7g014330 38.758 467 251 15 18 466 3 452 1.85e-93 291
MS.gene043348.t1 MTR_2g089650 36.765 476 258 15 17 470 9 463 3.24e-90 283
MS.gene043348.t1 MTR_6g015850 34.917 484 280 10 1 473 1 460 1.03e-87 276
MS.gene043348.t1 MTR_6g015815 35.381 472 272 11 18 473 9 463 7.09e-87 274
MS.gene043348.t1 MTR_2g089755 36.419 497 267 17 1 470 1 475 5.25e-86 272
MS.gene043348.t1 MTR_7g014230 36.910 466 261 14 18 466 5 454 7.03e-84 266
MS.gene043348.t1 MTR_7g014250 36.017 472 267 14 18 471 3 457 1.32e-83 265
MS.gene043348.t1 MTR_2g089735 35.698 437 247 12 54 470 39 461 4.08e-81 259
MS.gene043348.t1 MTR_3g087640 34.874 476 274 13 18 470 10 472 5.43e-81 259
MS.gene043348.t1 MTR_6g015830 34.110 472 282 11 16 473 7 463 9.96e-79 253
MS.gene043348.t1 MTR_6g015320 32.623 469 281 12 13 466 2 450 8.66e-75 242
MS.gene043348.t1 MTR_6g015315 34.118 425 257 8 52 466 37 448 1.79e-73 239
MS.gene043348.t1 MTR_7g014610 34.526 475 268 14 29 475 18 477 2.88e-72 236
MS.gene043348.t1 MTR_7g014160 31.942 479 289 11 12 466 4 469 4.51e-72 236
MS.gene043348.t1 MTR_7g014200 33.817 482 271 17 12 466 4 464 7.94e-72 235
MS.gene043348.t1 MTR_7g013850 31.906 467 265 14 41 470 30 480 3.53e-64 215
MS.gene043348.t1 MTR_7g014620 32.143 448 257 11 36 466 11 428 2.52e-59 201
MS.gene043348.t1 MTR_7g014360 30.435 391 233 10 86 466 21 382 2.40e-50 176
MS.gene043348.t1 MTR_3g435300 36.749 283 153 11 18 289 4 271 9.31e-43 152
MS.gene043348.t1 MTR_6g015310 26.902 368 206 9 88 448 22 333 5.20e-34 130
MS.gene043348.t1 MTR_7g014310 29.091 330 177 9 125 451 29 304 7.99e-34 129
MS.gene043348.t1 MTR_7g014670 36.757 185 113 2 286 466 71 255 1.39e-33 127
MS.gene043348.t1 MTR_2g089745 71.605 81 23 0 396 476 3 83 7.96e-30 112
MS.gene043348.t1 MTR_7g014630 31.359 287 164 11 17 286 9 279 1.48e-29 119
MS.gene043348.t1 MTR_7g014180 38.547 179 95 6 17 191 7 174 6.76e-25 101
MS.gene043348.t1 MTR_2g064495 25.238 420 275 15 57 466 39 429 3.78e-21 95.9
MS.gene043348.t1 MTR_2g105330 24.824 427 278 15 57 473 39 432 9.61e-21 94.7
MS.gene043348.t1 MTR_8g041770 28.117 377 232 14 55 422 44 390 3.17e-20 93.2
MS.gene043348.t1 MTR_4g022670 26.790 377 245 15 55 422 38 392 8.32e-20 92.0
MS.gene043348.t1 MTR_4g022720 24.364 472 294 18 16 465 3 433 7.97e-19 89.0
MS.gene043348.t1 MTR_4g094835 26.834 477 267 22 15 456 2 431 1.29e-18 88.6
MS.gene043348.t1 MTR_4g007540 23.866 419 275 15 57 462 39 426 2.89e-18 87.4
MS.gene043348.t1 MTR_8g079710 24.760 416 235 17 37 422 26 393 6.33e-18 86.3
MS.gene043348.t1 MTR_3g088420 25.647 425 255 15 29 437 15 394 6.90e-18 86.3
MS.gene043348.t1 MTR_4g094842 24.160 476 307 18 15 466 2 447 1.88e-17 85.1
MS.gene043348.t1 MTR_8g075610 27.177 379 229 17 57 422 30 374 2.35e-17 84.3
MS.gene043348.t1 MTR_4g022800 25.120 418 272 15 67 465 45 440 5.27e-17 83.6
MS.gene043348.t1 MTR_7g014210 37.931 116 68 3 328 440 24 138 6.34e-17 78.6
MS.gene043348.t1 MTR_3g070170 22.857 420 270 13 71 459 60 456 1.00e-16 82.8
MS.gene043348.t1 MTR_3g026310 26.108 406 251 14 38 422 22 399 6.65e-16 80.1
MS.gene043348.t1 MTR_7g014205 37.391 115 58 3 326 438 18 120 1.51e-15 73.2
MS.gene043348.t1 MTR_8g096530 25.062 403 248 14 76 462 58 422 2.30e-15 78.6
MS.gene043348.t1 MTR_7g085930 24.561 456 279 19 53 473 39 464 3.05e-15 78.2
MS.gene043348.t1 MTR_3g098980 24.623 398 255 15 76 462 65 428 4.60e-15 77.4
MS.gene043348.t1 MTR_3g435290 36.111 108 66 2 351 458 1 105 7.50e-15 71.2
MS.gene043348.t1 MTR_7g014340 59.420 69 26 2 18 85 3 70 1.17e-14 70.1
MS.gene043348.t1 MTR_4g022290 23.133 415 248 17 34 422 24 393 2.78e-14 75.1
MS.gene043348.t1 MTR_7g014220 59.375 64 25 1 32 95 10 72 6.96e-14 67.4
MS.gene043348.t1 MTR_7g085990 25.207 484 283 22 17 459 1 446 5.06e-13 71.2
MS.gene043348.t1 MTR_4g094828 24.079 407 256 17 36 422 25 398 5.78e-13 71.2
MS.gene043348.t1 MTR_2g007880 21.628 430 265 15 59 462 56 439 1.09e-12 70.1
MS.gene043348.t1 MTR_7g114560 21.333 375 251 13 57 421 47 387 1.23e-12 70.1
MS.gene043348.t1 MTR_3g101930 24.332 374 242 16 63 421 56 403 1.28e-12 70.1
MS.gene043348.t1 MTR_8g079970 22.222 459 307 14 15 462 1 420 4.59e-12 68.2
MS.gene043348.t1 MTR_4g097090 23.907 389 245 13 57 421 45 406 1.82e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043348.t1 AT1G03940 37.792 471 254 14 18 467 9 461 6.66e-100 308
MS.gene043348.t1 AT3G29670 34.402 468 276 15 15 470 1 449 1.51e-72 236
MS.gene043348.t1 AT1G03495 39.193 347 188 12 18 353 9 343 2.39e-68 222
MS.gene043348.t1 AT5G61160 31.712 473 287 14 15 472 1 452 3.94e-63 212
MS.gene043348.t1 AT3G29635 31.343 469 294 12 15 470 1 454 1.03e-61 208
MS.gene043348.t1 AT3G29680 31.360 456 296 9 15 466 1 443 6.28e-60 203
MS.gene043348.t1 AT5G39090 31.712 473 273 17 16 468 4 446 3.56e-57 196
MS.gene043348.t1 AT5G39080 29.707 478 293 18 16 470 4 461 3.00e-55 191
MS.gene043348.t1 AT3G29590 31.677 483 261 20 12 466 3 444 3.04e-54 188
MS.gene043348.t1 AT5G39050 29.079 478 294 14 16 468 9 466 9.33e-50 177
MS.gene043348.t1 AT5G23940 26.598 485 277 22 15 463 1 442 1.19e-22 100
MS.gene043348.t1 AT2G19070 24.018 433 271 15 59 462 40 443 3.80e-18 87.0
MS.gene043348.t1 AT3G48720 22.170 424 285 13 54 467 41 429 1.01e-17 85.9
MS.gene043348.t1 AT5G38130 23.840 474 304 19 9 463 16 451 1.78e-17 85.1
MS.gene043348.t1 AT3G29690 30.201 149 98 2 319 466 31 174 6.54e-17 79.0
MS.gene043348.t1 AT3G50300 25.701 428 257 17 17 422 4 392 4.97e-16 80.5
MS.gene043348.t1 AT3G50280 25.412 425 256 16 20 422 7 392 1.86e-15 79.0
MS.gene043348.t1 AT5G42830 24.664 446 274 20 36 464 36 436 2.97e-15 78.2
MS.gene043348.t1 AT5G41040 23.967 363 234 13 71 422 63 394 4.13e-15 77.8
MS.gene043348.t1 AT5G41040 23.967 363 234 13 71 422 79 410 5.46e-15 77.4
MS.gene043348.t1 AT3G50270 23.272 434 259 17 19 422 8 397 9.68e-15 76.6
MS.gene043348.t1 AT3G29636 29.592 196 81 7 262 455 27 167 7.83e-14 70.5
MS.gene043348.t1 AT5G01210 24.848 330 212 13 161 464 141 460 6.72e-12 67.8
MS.gene043348.t1 AT1G31490 23.097 381 237 13 58 422 43 383 1.48e-11 66.6

Find 101 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTTTCTTTGACATTTCT+TGG 0.109669 2.2:-10218841 MS.gene043348:CDS
AACTCTGTTAAAATGGTTTC+TGG 0.155098 2.2:-10217765 MS.gene043348:CDS
TCATTACCGAAAACTTATTT+TGG 0.167042 2.2:-10217960 MS.gene043348:CDS
ACCAGGTAACTCTGTTAAAA+TGG 0.224927 2.2:-10217772 MS.gene043348:CDS
GGAGGGGTGTTATGGGAATT+TGG 0.229278 2.2:+10218665 None:intergenic
GGTAGAAGAAGATGCGTTTA+AGG 0.233797 2.2:+10218801 None:intergenic
GATGTTTATGAGACTGATTT+TGG 0.265860 2.2:-10217729 MS.gene043348:CDS
AAACGTTGATGCCTGATTAT+AGG 0.285361 2.2:-10217806 MS.gene043348:CDS
ATTCACAAGATGCTCTAAAT+TGG 0.287826 2.2:-10218562 MS.gene043348:CDS
GTTGGTGATGGAAGTGAATT+TGG 0.295109 2.2:+10218875 None:intergenic
GTAAAGAGAGATGAGTTAAT+TGG 0.307415 2.2:-10217909 MS.gene043348:CDS
TGCACTACATCAGATTTCTT+TGG 0.324816 2.2:+10217696 None:intergenic
TTTATTATTTCCCTATAATC+AGG 0.339043 2.2:+10217795 None:intergenic
TTGTAACATGTTCCTTGATT+TGG 0.350808 2.2:-10218067 MS.gene043348:CDS
GATTTCATCAATATCTATAA+AGG 0.352392 2.2:-10217579 MS.gene043348:CDS
GTATATAAGGAGGGGTGTTA+TGG 0.354673 2.2:+10218657 None:intergenic
AACGTTGATGCCTGATTATA+GGG 0.364124 2.2:-10217805 MS.gene043348:CDS
TCTTGTAAGTAGGTGAGTTT+AGG 0.364713 2.2:+10218260 None:intergenic
GTTGTTGTTTGCTATAGCTT+TGG 0.366415 2.2:+10218355 None:intergenic
GAAGAAGTGTTGGAGGGTTA+AGG 0.375241 2.2:+10218715 None:intergenic
TTATAGATATTGATGAAATC+AGG 0.399480 2.2:+10217582 None:intergenic
GTATGAGGTGAAGGCAAGTT+AGG 0.400483 2.2:+10218527 None:intergenic
CATACAGAGAAGCCAGAGTT+TGG 0.410447 2.2:+10218449 None:intergenic
TATATAAGGAGGGGTGTTAT+GGG 0.421407 2.2:+10218658 None:intergenic
ATTTGGAGGGATGATGAGGT+TGG 0.424629 2.2:+10218682 None:intergenic
GGAAAGTGAGTGTTTGAGAA+TGG 0.424878 2.2:+10218736 None:intergenic
GATGAGTTGGATGTGGAAAA+TGG 0.428885 2.2:+10218780 None:intergenic
GATGATGAGGTTGGAGGTGA+AGG 0.429029 2.2:+10218691 None:intergenic
GAAGCCAGAGTTTGGTAGAA+TGG 0.432626 2.2:+10218457 None:intergenic
TTTAAGAGTGATGCTTTGTT+GGG 0.435490 2.2:-10217834 MS.gene043348:CDS
TCATCAAGGTAACGTATATA+AGG 0.436544 2.2:+10218644 None:intergenic
TCTCTTTCTGATTGTAGAGA+TGG 0.439448 2.2:-10217651 MS.gene043348:CDS
GTCTCATAAACATCAAGTTT+AGG 0.448315 2.2:+10217738 None:intergenic
AGGTGGAATTGAAGTTGGTT+TGG 0.449191 2.2:-10217625 MS.gene043348:CDS
TGAGAATGGGAAGTGTTGTT+TGG 0.450918 2.2:+10218750 None:intergenic
GTAGAAGAAGATGCGTTTAA+GGG 0.457685 2.2:+10218802 None:intergenic
TACCATACAAAACCAAATCA+AGG 0.462671 2.2:+10218055 None:intergenic
ACTCTGTTAAAATGGTTTCT+GGG 0.468209 2.2:-10217764 MS.gene043348:CDS
GAAAGTGAGTGTTTGAGAAT+GGG 0.469129 2.2:+10218737 None:intergenic
CTTTCTGATTGTAGAGATGG+TGG 0.471103 2.2:-10217648 MS.gene043348:CDS
AGGTGAAGGGGAAGAAGTGT+TGG 0.471582 2.2:+10218705 None:intergenic
AAAGAAAGTGAGAGGGAGAA+TGG 0.474809 2.2:+10218853 None:intergenic
GGTGGAGGTGGAATTGAAGT+TGG 0.477895 2.2:-10217630 MS.gene043348:CDS
GGGAATTTGGAGGGATGATG+AGG 0.494796 2.2:+10218678 None:intergenic
GAAGAAGATGCGTTTAAGGG+GGG 0.500670 2.2:+10218805 None:intergenic
ATCAGAAAGAGAAATCGAAC+TGG 0.507399 2.2:+10217662 None:intergenic
AAAGTGAGAGGGAGAATGGT+TGG 0.508062 2.2:+10218857 None:intergenic
TAGAAGAAGATGCGTTTAAG+GGG 0.508756 2.2:+10218803 None:intergenic
GGTGATGGAAGTGAATTTGG+TGG 0.516073 2.2:+10218878 None:intergenic
TGGTAGAATGGTGATTTGAA+TGG 0.518937 2.2:+10218469 None:intergenic
GATGAGTTAATTGGAAAAGA+CGG 0.519523 2.2:-10217900 MS.gene043348:CDS
TAGGTGAGTTTAGGATCCGT+TGG 0.520040 2.2:+10218269 None:intergenic
TGATGAGGTTGGAGGTGAAG+GGG 0.522375 2.2:+10218693 None:intergenic
GGGGTGTTATGGGAATTTGG+AGG 0.522455 2.2:+10218668 None:intergenic
TACATGTATCGTTCCCCTCA+TGG 0.524239 2.2:-10218492 MS.gene043348:CDS
TGAAGGGGAAGAAGTGTTGG+AGG 0.527422 2.2:+10218708 None:intergenic
ATGATGAGGTTGGAGGTGAA+GGG 0.530666 2.2:+10218692 None:intergenic
AGAGGGAGAATGGTTGGTGA+TGG 0.530934 2.2:+10218863 None:intergenic
AGAAGAAGATGCGTTTAAGG+GGG 0.531371 2.2:+10218804 None:intergenic
AGGTGAGTTTAGGATCCGTT+GGG 0.537324 2.2:+10218270 None:intergenic
GATTGTAGAGATGGTGGAGG+TGG 0.541712 2.2:-10217642 MS.gene043348:CDS
TGGAGGGATGATGAGGTTGG+AGG 0.545689 2.2:+10218685 None:intergenic
GTTTGGAGGAAGTGATGAGT+TGG 0.551364 2.2:+10218767 None:intergenic
TTGTCTTGCATCTTATATTG+TGG 0.552776 2.2:-10217934 MS.gene043348:CDS
AATCTATCTCTTTCATGAGA+AGG 0.553262 2.2:+10218302 None:intergenic
AAGAAGATGCGTTTAAGGGG+GGG 0.554836 2.2:+10218806 None:intergenic
TTTAAACACGTCGTTGCAGA+CGG 0.556810 2.2:-10218419 MS.gene043348:CDS
TAACGAAACAGTAACTATGA+AGG 0.559253 2.2:-10218927 MS.gene043348:CDS
AATATCTATAAAGGACAACT+TGG 0.562582 2.2:-10217570 MS.gene043348:CDS
TGGAAAAGACGGTATTGTTG+TGG 0.564685 2.2:-10217889 MS.gene043348:CDS
GAAATGTCAAAGAAAGTGAG+AGG 0.565539 2.2:+10218845 None:intergenic
AGGAAGTGATGAGTTGGATG+TGG 0.573881 2.2:+10218773 None:intergenic
ATCACCATTCTACCAAACTC+TGG 0.574194 2.2:-10218461 MS.gene043348:CDS
TGCATCTCGAACGAGACTTG+TGG 0.575560 2.2:+10218205 None:intergenic
CAAGGTAACGTATATAAGGA+GGG 0.577627 2.2:+10218648 None:intergenic
AATTTGATCTCGTGATGTTG+AGG 0.584229 2.2:+10218956 None:intergenic
AAATGTCAAAGAAAGTGAGA+GGG 0.588095 2.2:+10218846 None:intergenic
TGGCAGCAAATGCTATTGAA+AGG 0.588748 2.2:-10217869 MS.gene043348:CDS
GGGTGTTATGGGAATTTGGA+GGG 0.588819 2.2:+10218669 None:intergenic
TCAAGGTAACGTATATAAGG+AGG 0.593641 2.2:+10218647 None:intergenic
GAATGGGAAGTGTTGTTTGG+AGG 0.595204 2.2:+10218753 None:intergenic
GGTGATTTGAATGGCCATGA+GGG 0.602488 2.2:+10218478 None:intergenic
TCACCTCATACAGAACAAAA+CGG 0.603095 2.2:-10218515 MS.gene043348:CDS
ATCTTGTATATCTTGTAAGT+AGG 0.603551 2.2:+10218250 None:intergenic
TGAGGATTCAGCAATGGTGA+AGG 0.609931 2.2:+10218610 None:intergenic
TCATGAGAAGGAAGATCAAG+AGG 0.612643 2.2:+10218314 None:intergenic
GATGGAAGTGAATTTGGTGG+TGG 0.624086 2.2:+10218881 None:intergenic
TCTGATTGTAGAGATGGTGG+AGG 0.625713 2.2:-10217645 MS.gene043348:CDS
TGAGAAGGAAGATCAAGAGG+TGG 0.626715 2.2:+10218317 None:intergenic
GAAGGGGAAGAAGTGTTGGA+GGG 0.639354 2.2:+10218709 None:intergenic
TAGATTACTAAAACACCCAA+CGG 0.650878 2.2:-10218285 MS.gene043348:CDS
AAGGCAAGTTAGGAACAAGA+TGG 0.656225 2.2:+10218537 None:intergenic
GTCTGCTGAGGATTCAGCAA+TGG 0.656353 2.2:+10218604 None:intergenic
AGGAGATAGAGTCTTCATCA+AGG 0.656629 2.2:+10218630 None:intergenic
TAGTTTAGTATTGAGTCGTG+AGG 0.661773 2.2:-10218159 MS.gene043348:CDS
TATAGGGAAATAATAAAACC+AGG 0.671327 2.2:-10217789 MS.gene043348:CDS
AAGGTAACGTATATAAGGAG+GGG 0.677863 2.2:+10218649 None:intergenic
TGGTGATTTGAATGGCCATG+AGG 0.702362 2.2:+10218477 None:intergenic
GTGATTTGAATGGCCATGAG+GGG 0.704235 2.2:+10218479 None:intergenic
GAGAATGTTGAAGTCTGCTG+AGG 0.705294 2.2:+10218592 None:intergenic
ACAACAAAAGTTGACATGTG+TGG 0.716536 2.2:+10218086 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATCATTTTATGAAATTTT+GGG - chr2.2:10218123-10218142 MS.gene043348:CDS 15.0%
!!! TTCATCATTTTATGAAATTT+TGG - chr2.2:10218122-10218141 MS.gene043348:CDS 15.0%
!! GATTTCATCAATATCTATAA+AGG - chr2.2:10218931-10218950 MS.gene043348:CDS 20.0%
!! TTATAGATATTGATGAAATC+AGG + chr2.2:10218931-10218950 None:intergenic 20.0%
!! TTTATTATTTCCCTATAATC+AGG + chr2.2:10218718-10218737 None:intergenic 20.0%
!!! AGATATACAAGATTTTGTAT+TGG - chr2.2:10218270-10218289 MS.gene043348:CDS 20.0%
!!! CAATTTCCAAAATAAGTTTT+CGG + chr2.2:10218559-10218578 None:intergenic 20.0%
! AAGTTCAAAAAATGAAGAGA+TGG - chr2.2:10218374-10218393 MS.gene043348:CDS 25.0%
! AATATCTATAAAGGACAACT+TGG - chr2.2:10218940-10218959 MS.gene043348:CDS 25.0%
! AGTTCAAAAAATGAAGAGAT+GGG - chr2.2:10218375-10218394 MS.gene043348:CDS 25.0%
! ATCTTGTATATCTTGTAAGT+AGG + chr2.2:10218263-10218282 None:intergenic 25.0%
! TATAGGGAAATAATAAAACC+AGG - chr2.2:10218721-10218740 MS.gene043348:CDS 25.0%
!! AGATTTTGTATTGGAAATCA+TGG - chr2.2:10218279-10218298 MS.gene043348:CDS 25.0%
!! TCATTACCGAAAACTTATTT+TGG - chr2.2:10218550-10218569 MS.gene043348:CDS 25.0%
!!! ATGTTTATGAGACTGATTTT+GGG - chr2.2:10218782-10218801 MS.gene043348:CDS 25.0%
AAATGTCAAAGAAAGTGAGA+GGG + chr2.2:10217667-10217686 None:intergenic 30.0%
AACTCTGTTAAAATGGTTTC+TGG - chr2.2:10218745-10218764 MS.gene043348:CDS 30.0%
AATCTATCTCTTTCATGAGA+AGG + chr2.2:10218211-10218230 None:intergenic 30.0%
ACTCTGTTAAAATGGTTTCT+GGG - chr2.2:10218746-10218765 MS.gene043348:CDS 30.0%
GATGAGTTAATTGGAAAAGA+CGG - chr2.2:10218610-10218629 MS.gene043348:CDS 30.0%
GTAAAGAGAGATGAGTTAAT+TGG - chr2.2:10218601-10218620 MS.gene043348:CDS 30.0%
GTCTCATAAACATCAAGTTT+AGG + chr2.2:10218775-10218794 None:intergenic 30.0%
TAACGAAACAGTAACTATGA+AGG - chr2.2:10217583-10217602 MS.gene043348:CDS 30.0%
TACCATACAAAACCAAATCA+AGG + chr2.2:10218458-10218477 None:intergenic 30.0%
TAGATTACTAAAACACCCAA+CGG - chr2.2:10218225-10218244 MS.gene043348:CDS 30.0%
TCACTTTCTTTGACATTTCT+TGG - chr2.2:10217669-10217688 MS.gene043348:CDS 30.0%
TCATCAAGGTAACGTATATA+AGG + chr2.2:10217869-10217888 None:intergenic 30.0%
TTGTAACATGTTCCTTGATT+TGG - chr2.2:10218443-10218462 MS.gene043348:CDS 30.0%
TTGTCTTGCATCTTATATTG+TGG - chr2.2:10218576-10218595 MS.gene043348:CDS 30.0%
! ATTCACAAGATGCTCTAAAT+TGG - chr2.2:10217948-10217967 MS.gene043348:CDS 30.0%
! GTTTTCGGTAATGAAAATTC+AGG + chr2.2:10218544-10218563 None:intergenic 30.0%
! TTTAAGAGTGATGCTTTGTT+GGG - chr2.2:10218676-10218695 MS.gene043348:CDS 30.0%
!! GATGTTTATGAGACTGATTT+TGG - chr2.2:10218781-10218800 MS.gene043348:CDS 30.0%
!! TTTTAAGAGTGATGCTTTGT+TGG - chr2.2:10218675-10218694 MS.gene043348:CDS 30.0%
!!! TTCCTTGATTTGGTTTTGTA+TGG - chr2.2:10218453-10218472 MS.gene043348:CDS 30.0%
AAACGTTGATGCCTGATTAT+AGG - chr2.2:10218704-10218723 MS.gene043348:CDS 35.0%
AACGTTGATGCCTGATTATA+GGG - chr2.2:10218705-10218724 MS.gene043348:CDS 35.0%
AAGGTAACGTATATAAGGAG+GGG + chr2.2:10217864-10217883 None:intergenic 35.0%
AATTTGATCTCGTGATGTTG+AGG + chr2.2:10217557-10217576 None:intergenic 35.0%
ACAACAAAAGTTGACATGTG+TGG + chr2.2:10218427-10218446 None:intergenic 35.0%
ACCAGGTAACTCTGTTAAAA+TGG - chr2.2:10218738-10218757 MS.gene043348:CDS 35.0%
ATCAGAAAGAGAAATCGAAC+TGG + chr2.2:10218851-10218870 None:intergenic 35.0%
CAAGGTAACGTATATAAGGA+GGG + chr2.2:10217865-10217884 None:intergenic 35.0%
GAAAGTGAGTGTTTGAGAAT+GGG + chr2.2:10217776-10217795 None:intergenic 35.0%
GAAATGTCAAAGAAAGTGAG+AGG + chr2.2:10217668-10217687 None:intergenic 35.0%
GTAGAAGAAGATGCGTTTAA+GGG + chr2.2:10217711-10217730 None:intergenic 35.0%
TAGAAGAAGATGCGTTTAAG+GGG + chr2.2:10217710-10217729 None:intergenic 35.0%
TCAAGGTAACGTATATAAGG+AGG + chr2.2:10217866-10217885 None:intergenic 35.0%
TCACCTCATACAGAACAAAA+CGG - chr2.2:10217995-10218014 MS.gene043348:CDS 35.0%
TCTCTTTCTGATTGTAGAGA+TGG - chr2.2:10218859-10218878 MS.gene043348:CDS 35.0%
TCTTGTAAGTAGGTGAGTTT+AGG + chr2.2:10218253-10218272 None:intergenic 35.0%
TGCACTACATCAGATTTCTT+TGG + chr2.2:10218817-10218836 None:intergenic 35.0%
TGGTAGAATGGTGATTTGAA+TGG + chr2.2:10218044-10218063 None:intergenic 35.0%
! TAGTTTAGTATTGAGTCGTG+AGG - chr2.2:10218351-10218370 MS.gene043348:CDS 35.0%
! TTTTGTTCTGTATGAGGTGA+AGG + chr2.2:10217995-10218014 None:intergenic 35.0%
!! ACCATTTTAACAGAGTTACC+TGG + chr2.2:10218742-10218761 None:intergenic 35.0%
!! GTTGTTGTTTGCTATAGCTT+TGG + chr2.2:10218158-10218177 None:intergenic 35.0%
!! TATATAAGGAGGGGTGTTAT+GGG + chr2.2:10217855-10217874 None:intergenic 35.0%
!! TTATGAGACTGATTTTGGGT+GGG - chr2.2:10218786-10218805 MS.gene043348:CDS 35.0%
!! TTTATGAGACTGATTTTGGG+TGG - chr2.2:10218785-10218804 MS.gene043348:CDS 35.0%
AAAGAAAGTGAGAGGGAGAA+TGG + chr2.2:10217660-10217679 None:intergenic 40.0%
AAGGCAAGTTAGGAACAAGA+TGG + chr2.2:10217976-10217995 None:intergenic 40.0%
AGAAGAAGATGCGTTTAAGG+GGG + chr2.2:10217709-10217728 None:intergenic 40.0%
AGGAGATAGAGTCTTCATCA+AGG + chr2.2:10217883-10217902 None:intergenic 40.0%
ATCACCATTCTACCAAACTC+TGG - chr2.2:10218049-10218068 MS.gene043348:CDS 40.0%
CTTTCTGATTGTAGAGATGG+TGG - chr2.2:10218862-10218881 MS.gene043348:CDS 40.0%
GGAAAGTGAGTGTTTGAGAA+TGG + chr2.2:10217777-10217796 None:intergenic 40.0%
GGTAGAAGAAGATGCGTTTA+AGG + chr2.2:10217712-10217731 None:intergenic 40.0%
TCATGAGAAGGAAGATCAAG+AGG + chr2.2:10218199-10218218 None:intergenic 40.0%
TGGAAAAGACGGTATTGTTG+TGG - chr2.2:10218621-10218640 MS.gene043348:CDS 40.0%
TGGCAGCAAATGCTATTGAA+AGG - chr2.2:10218641-10218660 MS.gene043348:CDS 40.0%
TTTAAACACGTCGTTGCAGA+CGG - chr2.2:10218091-10218110 MS.gene043348:CDS 40.0%
! AGGTGGAATTGAAGTTGGTT+TGG - chr2.2:10218885-10218904 MS.gene043348:CDS 40.0%
! GATGAGTTGGATGTGGAAAA+TGG + chr2.2:10217733-10217752 None:intergenic 40.0%
!! GTACCGTTTTGTTCTGTATG+AGG + chr2.2:10218001-10218020 None:intergenic 40.0%
!! GTATATAAGGAGGGGTGTTA+TGG + chr2.2:10217856-10217875 None:intergenic 40.0%
!! GTTGGTGATGGAAGTGAATT+TGG + chr2.2:10217638-10217657 None:intergenic 40.0%
!! TATGAGACTGATTTTGGGTG+GGG - chr2.2:10218787-10218806 MS.gene043348:CDS 40.0%
!! TGAGAATGGGAAGTGTTGTT+TGG + chr2.2:10217763-10217782 None:intergenic 40.0%
AAAGTGAGAGGGAGAATGGT+TGG + chr2.2:10217656-10217675 None:intergenic 45.0%
AAGAAGATGCGTTTAAGGGG+GGG + chr2.2:10217707-10217726 None:intergenic 45.0%
ATGATGAGGTTGGAGGTGAA+GGG + chr2.2:10217821-10217840 None:intergenic 45.0%
ATTTGGAGGGATGATGAGGT+TGG + chr2.2:10217831-10217850 None:intergenic 45.0%
CATACAGAGAAGCCAGAGTT+TGG + chr2.2:10218064-10218083 None:intergenic 45.0%
GAAGAAGATGCGTTTAAGGG+GGG + chr2.2:10217708-10217727 None:intergenic 45.0%
GAAGCCAGAGTTTGGTAGAA+TGG + chr2.2:10218056-10218075 None:intergenic 45.0%
GAGAATGTTGAAGTCTGCTG+AGG + chr2.2:10217921-10217940 None:intergenic 45.0%
GGTGATTTGAATGGCCATGA+GGG + chr2.2:10218035-10218054 None:intergenic 45.0%
GTATGAGGTGAAGGCAAGTT+AGG + chr2.2:10217986-10218005 None:intergenic 45.0%
GTGATTTGAATGGCCATGAG+GGG + chr2.2:10218034-10218053 None:intergenic 45.0%
GTTTGGAGGAAGTGATGAGT+TGG + chr2.2:10217746-10217765 None:intergenic 45.0%
TACATGTATCGTTCCCCTCA+TGG - chr2.2:10218018-10218037 MS.gene043348:CDS 45.0%
TGAGAAGGAAGATCAAGAGG+TGG + chr2.2:10218196-10218215 None:intergenic 45.0%
TGAGGATTCAGCAATGGTGA+AGG + chr2.2:10217903-10217922 None:intergenic 45.0%
TGGTGATTTGAATGGCCATG+AGG + chr2.2:10218036-10218055 None:intergenic 45.0%
! AGGAAGTGATGAGTTGGATG+TGG + chr2.2:10217740-10217759 None:intergenic 45.0%
! AGGTGAGTTTAGGATCCGTT+GGG + chr2.2:10218243-10218262 None:intergenic 45.0%
! GATGGAAGTGAATTTGGTGG+TGG + chr2.2:10217632-10217651 None:intergenic 45.0%
! GGGTGTTATGGGAATTTGGA+GGG + chr2.2:10217844-10217863 None:intergenic 45.0%
! TAGGTGAGTTTAGGATCCGT+TGG + chr2.2:10218244-10218263 None:intergenic 45.0%
! TCTGATTGTAGAGATGGTGG+AGG - chr2.2:10218865-10218884 MS.gene043348:CDS 45.0%
!! GAAGAAGTGTTGGAGGGTTA+AGG + chr2.2:10217798-10217817 None:intergenic 45.0%
!! GAATGGGAAGTGTTGTTTGG+AGG + chr2.2:10217760-10217779 None:intergenic 45.0%
!! GGTGATGGAAGTGAATTTGG+TGG + chr2.2:10217635-10217654 None:intergenic 45.0%
GATGATGAGGTTGGAGGTGA+AGG + chr2.2:10217822-10217841 None:intergenic 50.0%
GGGAATTTGGAGGGATGATG+AGG + chr2.2:10217835-10217854 None:intergenic 50.0%
GGTGGAGGTGGAATTGAAGT+TGG - chr2.2:10218880-10218899 MS.gene043348:CDS 50.0%
GTCTGCTGAGGATTCAGCAA+TGG + chr2.2:10217909-10217928 None:intergenic 50.0%
TGATGAGGTTGGAGGTGAAG+GGG + chr2.2:10217820-10217839 None:intergenic 50.0%
TGCATCTCGAACGAGACTTG+TGG + chr2.2:10218308-10218327 None:intergenic 50.0%
! AGGTGAAGGGGAAGAAGTGT+TGG + chr2.2:10217808-10217827 None:intergenic 50.0%
! GGGGTGTTATGGGAATTTGG+AGG + chr2.2:10217845-10217864 None:intergenic 50.0%
!! AGAGGGAGAATGGTTGGTGA+TGG + chr2.2:10217650-10217669 None:intergenic 50.0%
!! GAAGGGGAAGAAGTGTTGGA+GGG + chr2.2:10217804-10217823 None:intergenic 50.0%
!! GATTGTAGAGATGGTGGAGG+TGG - chr2.2:10218868-10218887 MS.gene043348:CDS 50.0%
!! GGAGGGGTGTTATGGGAATT+TGG + chr2.2:10217848-10217867 None:intergenic 50.0%
!! TGAAGGGGAAGAAGTGTTGG+AGG + chr2.2:10217805-10217824 None:intergenic 50.0%
TGGAGGGATGATGAGGTTGG+AGG + chr2.2:10217828-10217847 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 10217551 10218981 10217551 ID=MS.gene043348
chr2.2 mRNA 10217551 10218981 10217551 ID=MS.gene043348.t1;Parent=MS.gene043348
chr2.2 exon 10217551 10218981 10217551 ID=MS.gene043348.t1.exon1;Parent=MS.gene043348.t1
chr2.2 CDS 10217551 10218981 10217551 ID=cds.MS.gene043348.t1;Parent=MS.gene043348.t1
Gene Sequence

>MS.gene043348

ATGCCTCAACATCACGAGATCAAATTCTATCATAACGAAACAGTAACTATGAAGGTGATAGAGCAGAGTCAAGTTTCACCACCACCAAATTCACTTCCATCACCAACCATTCTCCCTCTCACTTTCTTTGACATTTCTTGGTTTTACTGCAAACCCCCCCTTAAACGCATCTTCTTCTACCATTTTCCACATCCAACTCATCACTTCCTCCAAACAACACTTCCCATTCTCAAACACTCACTTTCCTTAACCCTCCAACACTTCTTCCCCTTCACCTCCAACCTCATCATCCCTCCAAATTCCCATAACACCCCTCCTTATATACGTTACCTTGATGAAGACTCTATCTCCTTCACCATTGCTGAATCCTCAGCAGACTTCAACATTCTCATATCTGATTCACAAGATGCTCTAAATTGGCACCATCTTGTTCCTAACTTGCCTTCACCTCATACAGAACAAAACGGTACATGTATCGTTCCCCTCATGGCCATTCAAATCACCATTCTACCAAACTCTGGCTTCTCTGTATGTCTCGCGTTTAAACACGTCGTTGCAGACGGAAAATCACTTCATCATTTTATGAAATTTTGGGCATTTGTTTCCAAAGCTATAGCAAACAACAACGATTTGTCTCATTCTCCACCTCTTGATCTTCCTTCTCATGAAAGAGATAGATTACTAAAACACCCAACGGATCCTAAACTCACCTACTTACAAGATATACAAGATTTTGTATTGGAAATCATGGAATCCACAAGTCTCGTTCGAGATGCATCTTACACTAACAAAGTACGAACTAGTTTAGTATTGAGTCGTGAGGAAGTTCAAAAAATGAAGAGATGGGTCACTGATAAATGTAAAGACTCAACACCACACATGTCAACTTTTGTTGTAACATGTTCCTTGATTTGGTTTTGTATGGTAAAATCAGAGCAAAGAAAAAGTGATCTAGTGTACTTCTTGTTTTTTGCAGATTGTCGTGATCGTCCTGAATTTTCATTACCGAAAACTTATTTTGGAAATTGTCTTGCATCTTATATTGTGGCTGTAAAGAGAGATGAGTTAATTGGAAAAGACGGTATTGTTGTGGCAGCAAATGCTATTGAAAGGAAGATAAGAGATTTTAAGAGTGATGCTTTGTTGGGAGTTGAAACGTTGATGCCTGATTATAGGGAAATAATAAAACCAGGTAACTCTGTTAAAATGGTTTCTGGGTCACCTAAACTTGATGTTTATGAGACTGATTTTGGGTGGGGAAAACCAAAGAAATCTGATGTAGTGCATATTGATTCGTCCAGTTCGATTTCTCTTTCTGATTGTAGAGATGGTGGAGGTGGAATTGAAGTTGGTTTGGCTCTTGAGATGTCTCAAATGCCTGATTTCATCAATATCTATAAAGGACAACTTGGTGATATTTGTAGCATGTGA
Protein sequence

>MS.gene043348.t1

MPQHHEIKFYHNETVTMKVIEQSQVSPPPNSLPSPTILPLTFFDISWFYCKPPLKRIFFYHFPHPTHHFLQTTLPILKHSLSLTLQHFFPFTSNLIIPPNSHNTPPYIRYLDEDSISFTIAESSADFNILISDSQDALNWHHLVPNLPSPHTEQNGTCIVPLMAIQITILPNSGFSVCLAFKHVVADGKSLHHFMKFWAFVSKAIANNNDLSHSPPLDLPSHERDRLLKHPTDPKLTYLQDIQDFVLEIMESTSLVRDASYTNKVRTSLVLSREEVQKMKRWVTDKCKDSTPHMSTFVVTCSLIWFCMVKSEQRKSDLVYFLFFADCRDRPEFSLPKTYFGNCLASYIVAVKRDELIGKDGIVVAANAIERKIRDFKSDALLGVETLMPDYREIIKPGNSVKMVSGSPKLDVYETDFGWGKPKKSDVVHIDSSSSISLSDCRDGGGGIEVGLALEMSQMPDFINIYKGQLGDICSM