AlfalfaGEDB | MS.gene021913

M. sativa cultivar XinJiangDaYe / MS.gene021913

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021913.t1	XP_003621492.1	93.5	463	30	0	1	463	1	463	4.20E-255	890.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021913.t1	Q940Z5	40.0	463	258	10	5	462	7	454	1.5e-88	328.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021913.t1	F4ZG53	93.5	463	30	0	1	463	1	463	3.0e-255	890.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene073299	MS.gene021913	PPI
MS.gene50382	MS.gene021913	PPI
MS.gene91597	MS.gene021913	PPI
MS.gene021916	MS.gene021913	PPI
MS.gene32261	MS.gene021913	PPI
MS.gene31546	MS.gene021913	PPI
MS.gene021913	MS.gene96337	PPI
MS.gene99219	MS.gene021913	PPI
MS.gene021913	MS.gene87496	PPI
MS.gene021914	MS.gene021913	PPI
MS.gene021913	MS.gene070019	PPI
MS.gene021913	MS.gene021917	PPI
MS.gene90496	MS.gene021913	PPI
MS.gene56795	MS.gene021913	PPI
MS.gene021913	MS.gene91596	PPI
MS.gene066657	MS.gene021913	PPI
MS.gene56798	MS.gene021913	PPI
MS.gene002544	MS.gene021913	PPI
MS.gene069423	MS.gene021913	PPI
MS.gene78818	MS.gene021913	PPI
MS.gene000763	MS.gene021913	PPI
MS.gene021913	MS.gene56799	PPI
MS.gene91615	MS.gene021913	PPI
MS.gene021913	MS.gene44630	PPI
MS.gene50379	MS.gene021913	PPI
MS.gene38977	MS.gene021913	PPI
MS.gene066701	MS.gene021913	PPI
MS.gene44633	MS.gene021913	PPI
MS.gene021913	MS.gene011716	PPI
MS.gene021913	MS.gene91595	PPI
MS.gene90492	MS.gene021913	PPI
MS.gene91614	MS.gene021913	PPI
MS.gene91612	MS.gene021913	PPI
MS.gene48585	MS.gene021913	PPI
MS.gene021913	MS.gene016532	PPI
MS.gene021913	MS.gene043352	PPI
MS.gene021913	MS.gene50384	PPI
MS.gene021913	MS.gene44647	PPI
MS.gene011837	MS.gene021913	PPI
MS.gene021913	MS.gene066701	PPI
MS.gene066634	MS.gene021913	PPI
MS.gene35741	MS.gene021913	PPI
MS.gene021913	MS.gene021909	PPI
MS.gene043346	MS.gene021913	PPI
MS.gene91599	MS.gene021913	PPI
MS.gene91598	MS.gene021913	PPI
MS.gene021913	MS.gene011799	PPI
MS.gene49853	MS.gene021913	PPI
MS.gene063865	MS.gene021913	PPI
MS.gene021913	MS.gene50420	PPI
MS.gene021913	MS.gene064101	PPI
MS.gene021910	MS.gene021913	PPI
MS.gene028996	MS.gene021913	PPI
MS.gene004218	MS.gene021913	PPI
MS.gene28251	MS.gene021913	PPI
MS.gene91613	MS.gene021913	PPI
MS.gene021913	MS.gene50356	PPI
MS.gene35745	MS.gene021913	PPI
MS.gene011796	MS.gene021913	PPI
MS.gene011801	MS.gene021913	PPI
MS.gene87577	MS.gene021913	PPI
MS.gene021913	MS.gene87536	PPI
MS.gene021913	MS.gene56797	PPI
MS.gene87537	MS.gene021913	PPI
MS.gene000764	MS.gene021913	PPI
MS.gene021913	MS.gene99218	PPI
MS.gene48582	MS.gene021913	PPI
MS.gene90493	MS.gene021913	PPI
MS.gene90491	MS.gene021913	PPI
MS.gene013787	MS.gene021913	PPI
MS.gene021906	MS.gene021913	PPI
MS.gene31542	MS.gene021913	PPI
MS.gene87532	MS.gene021913	PPI
MS.gene99223	MS.gene021913	PPI
MS.gene46265	MS.gene021913	PPI
MS.gene063868	MS.gene021913	PPI
MS.gene066658	MS.gene021913	PPI
MS.gene48581	MS.gene021913	PPI
MS.gene87191	MS.gene021913	PPI
MS.gene021913	MS.gene35740	PPI
MS.gene99221	MS.gene021913	PPI
MS.gene03202	MS.gene021913	PPI
MS.gene007831	MS.gene021913	PPI
MS.gene021909	MS.gene021913	PPI
MS.gene011799	MS.gene021913	PPI
MS.gene021913	MS.gene021905	PPI
MS.gene44642	MS.gene021913	PPI
MS.gene021913	MS.gene070270	PPI
MS.gene56796	MS.gene021913	PPI
MS.gene91609	MS.gene021913	PPI
MS.gene069767	MS.gene021913	PPI
MS.gene44644	MS.gene021913	PPI
MS.gene99222	MS.gene021913	PPI
MS.gene02573	MS.gene021913	PPI
MS.gene60208	MS.gene021913	PPI
MS.gene011798	MS.gene021913	PPI
MS.gene48584	MS.gene021913	PPI
MS.gene032349	MS.gene021913	PPI
MS.gene021913	MS.gene87191	PPI
MS.gene066656	MS.gene021913	PPI
MS.gene44630	MS.gene021913	PPI
MS.gene066660	MS.gene021913	PPI
MS.gene31540	MS.gene021913	PPI
MS.gene021913	MS.gene016477	PPI
MS.gene44640	MS.gene021913	PPI
MS.gene44463	MS.gene021913	PPI
MS.gene021913	MS.gene02573	PPI
MS.gene50420	MS.gene021913	PPI
MS.gene043348	MS.gene021913	PPI
MS.gene021913	MS.gene028994	PPI
MS.gene87536	MS.gene021913	PPI
MS.gene50384	MS.gene021913	PPI
MS.gene021913	MS.gene87577	PPI
MS.gene069422	MS.gene021913	PPI
MS.gene012360	MS.gene021913	PPI
MS.gene35744	MS.gene021913	PPI
MS.gene44643	MS.gene021913	PPI
MS.gene028999	MS.gene021913	PPI
MS.gene028998	MS.gene021913	PPI
MS.gene31545	MS.gene021913	PPI
MS.gene021913	MS.gene043350	PPI
MS.gene021915	MS.gene021913	PPI
MS.gene50355	MS.gene021913	PPI
MS.gene91611	MS.gene021913	PPI
MS.gene50356	MS.gene021913	PPI
MS.gene021904	MS.gene021913	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021913.t1	MTR_7g014610	93.521	463	30	0	1	463	1	463	0.0	881
MS.gene021913.t1	MTR_7g014160	78.043	460	98	3	4	463	5	461	0.0	746
MS.gene021913.t1	MTR_7g014200	74.624	465	107	5	1	463	1	456	0.0	706
MS.gene021913.t1	MTR_7g014620	72.523	444	93	1	20	463	6	420	0.0	633
MS.gene021913.t1	MTR_7g013850	59.494	474	175	11	1	463	1	468	0.0	521
MS.gene021913.t1	MTR_7g014630	65.826	357	109	5	2	346	3	358	6.14e-160	457
MS.gene021913.t1	MTR_7g014330	41.595	464	236	12	9	461	3	442	5.19e-113	341
MS.gene021913.t1	MTR_7g014230	41.595	464	236	13	9	461	5	444	6.63e-111	335
MS.gene021913.t1	MTR_7g014250	39.869	459	251	10	9	461	3	442	1.91e-106	324
MS.gene021913.t1	MTR_6g015320	40.000	460	245	13	8	462	8	441	1.42e-105	322
MS.gene021913.t1	MTR_6g015815	38.248	468	262	9	1	462	1	447	1.86e-103	317
MS.gene021913.t1	MTR_6g015315	40.570	456	247	11	9	462	6	439	1.16e-102	314
MS.gene021913.t1	MTR_6g015850	39.180	439	240	10	25	461	30	443	2.41e-100	308
MS.gene021913.t1	MTR_6g015830	39.683	441	238	13	25	461	30	446	2.58e-99	306
MS.gene021913.t1	MTR_7g014670	74.719	178	44	1	286	463	71	247	1.01e-92	282
MS.gene021913.t1	MTR_7g014180	80.702	171	33	0	4	174	3	173	3.69e-91	275
MS.gene021913.t1	MTR_7g014360	35.969	392	202	8	78	461	22	372	1.69e-74	239
MS.gene021913.t1	MTR_3g028210	34.402	468	268	13	8	461	8	450	3.57e-73	238
MS.gene021913.t1	MTR_2g089765	33.881	487	261	17	5	461	13	468	2.35e-72	237
MS.gene021913.t1	MTR_7g015860	32.780	482	256	11	8	461	5	446	3.90e-68	225
MS.gene021913.t1	MTR_8g012750	32.468	462	276	12	18	461	15	458	8.88e-67	222
MS.gene021913.t1	MTR_2g089735	34.633	436	248	12	42	464	39	450	6.91e-65	216
MS.gene021913.t1	MTR_2g089670	33.705	448	261	13	25	461	41	463	1.24e-64	216
MS.gene021913.t1	MTR_3g087640	31.847	471	277	13	9	459	10	456	1.08e-60	206
MS.gene021913.t1	MTR_2g089650	32.347	473	271	15	5	459	6	447	3.36e-60	204
MS.gene021913.t1	MTR_6g015310	31.773	406	199	11	52	453	2	333	1.10e-57	194
MS.gene021913.t1	MTR_2g089755	32.739	449	258	13	29	459	37	459	6.89e-57	196
MS.gene021913.t1	MTR_7g014310	30.836	347	173	8	114	460	29	308	3.76e-42	152
MS.gene021913.t1	MTR_2g089665	27.253	455	196	14	8	449	2	334	2.67e-37	140
MS.gene021913.t1	MTR_3g435300	34.052	232	126	6	9	226	4	222	1.19e-33	128
MS.gene021913.t1	MTR_7g086000	23.504	468	303	11	7	453	3	436	6.85e-21	95.5
MS.gene021913.t1	MTR_4g007540	23.488	430	273	19	8	427	3	386	6.96e-18	86.3
MS.gene021913.t1	MTR_3g026950	24.000	425	253	19	63	457	49	433	1.26e-17	85.5
MS.gene021913.t1	MTR_4g094835	25.966	466	258	22	19	457	22	427	3.77e-17	84.0
MS.gene021913.t1	MTR_4g127650	23.355	471	306	17	8	461	10	442	1.75e-16	82.0
MS.gene021913.t1	MTR_5g081960	23.640	478	307	17	16	459	14	467	2.08e-16	82.0
MS.gene021913.t1	MTR_7g085930	22.926	458	273	18	5	427	3	415	4.03e-16	80.9
MS.gene021913.t1	MTR_7g014205	35.897	117	53	4	326	437	15	114	5.74e-16	74.3
MS.gene021913.t1	MTR_4g094828	23.983	467	280	21	15	457	16	431	6.08e-16	80.1
MS.gene021913.t1	MTR_2g015430	22.197	437	290	13	8	439	2	393	1.33e-15	79.3
MS.gene021913.t1	MTR_4g097090	23.272	434	270	16	14	426	15	406	2.06e-15	78.6
MS.gene021913.t1	MTR_7g014210	36.111	108	67	2	331	437	24	130	8.24e-15	72.4
MS.gene021913.t1	MTR_4g022825	25.243	412	241	20	68	457	55	421	5.21e-14	74.3
MS.gene021913.t1	MTR_7g085990	22.000	450	281	16	8	427	2	411	1.16e-13	73.2
MS.gene021913.t1	MTR_3g049150	22.385	478	293	21	8	457	2	429	1.84e-13	72.8
MS.gene021913.t1	MTR_3g098980	23.750	400	254	13	67	459	65	420	2.61e-13	72.0
MS.gene021913.t1	MTR_3g026310	25.527	427	235	21	65	457	52	429	3.21e-13	71.6
MS.gene021913.t1	MTR_7g084940	22.664	428	274	15	8	428	16	393	7.32e-13	70.9
MS.gene021913.t1	MTR_4g022290	23.385	449	272	17	32	460	33	429	7.88e-13	70.5
MS.gene021913.t1	MTR_7g014340	50.704	71	29	2	9	76	3	70	1.39e-12	64.3
MS.gene021913.t1	MTR_4g022720	24.638	414	236	17	68	457	55	416	8.68e-12	67.4
MS.gene021913.t1	MTR_3g435290	54.688	64	24	2	399	459	40	101	1.18e-11	62.4
MS.gene021913.t1	MTR_7g014220	51.852	54	23	1	25	78	14	64	8.01e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021913.t1	AT5G39050	40.086	464	256	12	5	462	7	454	1.54e-98	304
MS.gene021913.t1	AT5G39080	38.925	465	257	14	6	462	3	448	1.20e-97	301
MS.gene021913.t1	AT5G39090	37.719	456	243	14	15	462	12	434	3.11e-88	277
MS.gene021913.t1	AT3G29670	35.931	462	261	10	7	461	2	435	3.12e-87	274
MS.gene021913.t1	AT3G29590	36.285	463	250	10	8	461	8	434	2.71e-86	272
MS.gene021913.t1	AT3G29635	35.909	440	253	10	26	461	26	440	1.80e-84	267
MS.gene021913.t1	AT5G61160	32.026	459	282	11	8	461	3	436	4.13e-74	240
MS.gene021913.t1	AT3G29680	32.207	444	275	8	19	461	15	433	8.54e-70	229
MS.gene021913.t1	AT1G03940	31.897	464	264	14	18	461	19	450	4.98e-63	212
MS.gene021913.t1	AT1G03495	31.977	344	192	13	18	347	19	334	1.21e-41	152
MS.gene021913.t1	AT5G23940	24.946	465	273	19	29	466	25	440	1.18e-22	100
MS.gene021913.t1	AT3G29720	30.374	214	119	6	105	311	26	216	3.35e-18	84.0
MS.gene021913.t1	AT5G57840	22.917	480	296	22	8	466	3	429	8.51e-16	79.7
MS.gene021913.t1	AT1G28680	22.071	367	245	8	67	430	61	389	9.98e-16	79.7
MS.gene021913.t1	AT3G29690	30.216	139	89	4	324	461	33	164	7.11e-15	73.2
MS.gene021913.t1	AT5G41040	23.670	376	233	15	62	427	63	394	2.22e-14	75.5
MS.gene021913.t1	AT5G41040	23.670	376	233	15	62	427	79	410	2.55e-14	75.5
MS.gene021913.t1	AT1G28680	21.569	306	200	7	46	347	40	309	1.91e-13	72.4
MS.gene021913.t1	AT4G31910	25.000	324	179	13	45	345	46	328	1.69e-11	66.6
MS.gene021913.t1	AT3G47170	22.330	309	194	11	48	345	57	330	2.77e-11	65.9

Find 89 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACTTTCTTCGACATATTT+TGG	0.132884	7.1:-81783943	MS.gene021913:CDS
GAAGGGTGTGATTGTGAATT+TGG	0.182607	7.1:+81783878	None:intergenic
AGCAAATGGTGTTATCAAAA+TGG	0.193782	7.1:-81783214	MS.gene021913:CDS
TGAAAGGAATTGTTGTAGTT+TGG	0.220075	7.1:+81783963	None:intergenic
GAGAGTGAAGATTTGAGAAT+TGG	0.258335	7.1:+81783833	None:intergenic
CGAACGGAGTCTTCAAGTTT+TGG	0.272734	7.1:+81783278	None:intergenic
CAAAGTTGGTGATTCTTCTT+TGG	0.287688	7.1:+81783454	None:intergenic
CTTTAGCTGGTAAGATTGTT+TGG	0.295811	7.1:-81783787	MS.gene021913:CDS
AGCCATTCACCGAGCTGATA+TGG	0.316215	7.1:-81783099	MS.gene021913:CDS
GGTGTTTATGAGACTGATTT+TGG	0.320974	7.1:-81782783	MS.gene021913:CDS
ATGACTTCCCTATCGAATAA+AGG	0.325526	7.1:+81783416	None:intergenic
ATGATGTACCCAAACGAAAA+AGG	0.327194	7.1:-81783335	MS.gene021913:CDS
GAACAACTTTGAAGTGTTCA+TGG	0.335900	7.1:+81783999	None:intergenic
AATGTAACACCGAGTTCATT+TGG	0.341334	7.1:+81783386	None:intergenic
CACCATCATTTGTTGTGTAC+TGG	0.346313	7.1:+81783738	None:intergenic
ATCGAATAAAGGTTCTAAAT+CGG	0.346512	7.1:+81783427	None:intergenic
CCAACAAAGTTACTTGGTAT+TGG	0.364637	7.1:+81783020	None:intergenic
CCAATACCAAGTAACTTTGT+TGG	0.366378	7.1:-81783020	MS.gene021913:CDS
GAAAGGAATTGTTGTAGTTT+GGG	0.382255	7.1:+81783964	None:intergenic
CTTTGTTGGAAACTGTGTTT+GGG	0.394414	7.1:-81783006	MS.gene021913:CDS
TATAATTCTTTGATAAATGA+AGG	0.398505	7.1:-81782843	MS.gene021913:CDS
TCGTTCGTTTCTACCACATT+TGG	0.398985	7.1:-81783639	MS.gene021913:CDS
ACAAAAGATGATAAAGTTTG+TGG	0.412645	7.1:+81783158	None:intergenic
GCAAATGGTGTTATCAAAAT+GGG	0.418223	7.1:-81783213	MS.gene021913:CDS
ACTTTGTTGGAAACTGTGTT+TGG	0.421154	7.1:-81783007	MS.gene021913:CDS
AAGGGTGTGATTGTGAATTT+GGG	0.425297	7.1:+81783879	None:intergenic
AACCATGTTCATCAAAGCTT+GGG	0.425557	7.1:-81783504	MS.gene021913:CDS
TAGAGGTTTAACCATTGGTT+TGG	0.430246	7.1:-81782721	MS.gene021913:CDS
GTGGCGGGATCTAATAGATT+TGG	0.431741	7.1:-81782804	MS.gene021913:CDS
GGGAATGGTGGGGTTGAAGT+TGG	0.437053	7.1:-81782684	MS.gene021913:CDS
ACACACCATGCCGGTCTTGA+TGG	0.438910	7.1:-81783536	MS.gene021913:CDS
ATGTAACACCGAGTTCATTT+GGG	0.441397	7.1:+81783387	None:intergenic
TCGAATAAAGGTTCTAAATC+GGG	0.445157	7.1:+81783428	None:intergenic
TAGAAGAAGATTCGTTCTAT+AGG	0.446410	7.1:+81783908	None:intergenic
CAACACTTTCTCCCTTTAGC+TGG	0.448713	7.1:-81783800	MS.gene021913:CDS
CAAACAATCTTACCAGCTAA+AGG	0.455594	7.1:+81783788	None:intergenic
GGTTTGGCAGAGAGCAAAGA+TGG	0.455777	7.1:-81782705	MS.gene021913:CDS
CAACCATGTTCATCAAAGCT+TGG	0.466349	7.1:-81783505	MS.gene021913:CDS
CAGTTTCCAACAAAGTTACT+TGG	0.468590	7.1:+81783014	None:intergenic
CTCCATATCAGCTCGGTGAA+TGG	0.469556	7.1:+81783097	None:intergenic
AATGAGAACGGTGTTCTTGA+AGG	0.471924	7.1:-81782891	MS.gene021913:CDS
CTTCATCGATACACAACCAT+TGG	0.478861	7.1:-81782979	MS.gene021913:CDS
TTGGATTTCATAAAAGAAGA+TGG	0.479620	7.1:-81782960	MS.gene021913:CDS
TTTAGAACCTTTATTCGATA+GGG	0.486856	7.1:-81783423	MS.gene021913:CDS
GTGGATAGAGGTTTAACCAT+TGG	0.488225	7.1:-81782726	MS.gene021913:CDS
AAACAATCTTACCAGCTAAA+GGG	0.488781	7.1:+81783789	None:intergenic
ATTTAGAACCTTTATTCGAT+AGG	0.494976	7.1:-81783424	MS.gene021913:CDS
TTTGAACGAGATAAAGCAAA+TGG	0.496716	7.1:-81783228	MS.gene021913:CDS
ACAAAGTTACTTGGTATTGG+TGG	0.507710	7.1:+81783023	None:intergenic
TCTAAATCGGGTAACAAAGT+TGG	0.514035	7.1:+81783440	None:intergenic
GTGTTACATTCACAAATAGC+TGG	0.520246	7.1:-81783373	MS.gene021913:CDS
ATGAGAACGGTGTTCTTGAA+GGG	0.527012	7.1:-81782890	MS.gene021913:CDS
CAGCTAAAGGGAGAAAGTGT+TGG	0.529441	7.1:+81783801	None:intergenic
ATCCAGTACACAACAAATGA+TGG	0.530532	7.1:-81783740	MS.gene021913:CDS
GTAGTTTGGGTTGATGATGA+AGG	0.535398	7.1:+81783977	None:intergenic
AGAGCAAAGATGGGAATGGT+GGG	0.535501	7.1:-81782695	MS.gene021913:CDS
AGAAGATTCGTTCTATAGGA+TGG	0.538429	7.1:+81783912	None:intergenic
GAGAGCAAAGATGGGAATGG+TGG	0.542971	7.1:-81782696	MS.gene021913:CDS
CTTGAAGGGGCTATTAATGA+CGG	0.544900	7.1:-81782876	MS.gene021913:CDS
TTGAAGGGGCTATTAATGAC+GGG	0.545356	7.1:-81782875	MS.gene021913:CDS
TAACCTTGCTCTGCAATCTA+CGG	0.549902	7.1:+81783049	None:intergenic
GAGATAGTATCGGTGGATAG+AGG	0.550679	7.1:-81782738	MS.gene021913:CDS
TTTGGTTGTTGAGATTCTGA+TGG	0.557672	7.1:+81783764	None:intergenic
TTTGCTCTCTGCCAAACCAA+TGG	0.567957	7.1:+81782710	None:intergenic
CTAAAGGGAGAAAGTGTTGG+AGG	0.581010	7.1:+81783804	None:intergenic
GTACATCATATATATAATCT+CGG	0.585125	7.1:+81783349	None:intergenic
ATCAAAGACCCAAATGAACT+CGG	0.587395	7.1:-81783395	MS.gene021913:CDS
GAGATCGAATGTGGCACGAA+CGG	0.595168	7.1:+81783262	None:intergenic
ATCAGACTCTGCAATGAGCA+AGG	0.596030	7.1:+81783712	None:intergenic
GTTTGGCAGAGAGCAAAGAT+GGG	0.598479	7.1:-81782704	MS.gene021913:CDS
TCACCGTAGATTGCAGAGCA+AGG	0.598513	7.1:-81783052	MS.gene021913:CDS
AATATGTCGAAGAAAGTGAA+AGG	0.601558	7.1:+81783947	None:intergenic
ATAAAGATGATAAATGAGAA+CGG	0.603049	7.1:-81782903	MS.gene021913:CDS
GTGTGATTGTGAATTTGGGA+GGG	0.604440	7.1:+81783883	None:intergenic
AGCCCAAGCTTTGATGAACA+TGG	0.605776	7.1:+81783502	None:intergenic
GCAGAGAGCAAAGATGGGAA+TGG	0.607028	7.1:-81782699	MS.gene021913:CDS
AAAAGTCGAGATAGTATCGG+TGG	0.614890	7.1:-81782745	MS.gene021913:CDS
GAATCTGTTGATTCCAAATG+TGG	0.618036	7.1:+81783626	None:intergenic
GGTGTGATTGTGAATTTGGG+AGG	0.618307	7.1:+81783882	None:intergenic
TCTCTTTCTCCATATCAGCT+CGG	0.619042	7.1:+81783090	None:intergenic
TTCACGCGTGAGATCGAATG+TGG	0.619088	7.1:+81783253	None:intergenic
GGAATAAGCACACACCATGC+CGG	0.621157	7.1:-81783545	MS.gene021913:CDS
TGAGAACGGTGTTCTTGAAG+GGG	0.630071	7.1:-81782889	MS.gene021913:CDS
ATGGTGTGTGCTTATTCCAA+TGG	0.634350	7.1:+81783550	None:intergenic
TAAAGGGAGAAAGTGTTGGA+GGG	0.656830	7.1:+81783805	None:intergenic
CGAGACAAAGAAGCTTCATG+AGG	0.679550	7.1:+81783659	None:intergenic
GAGCAAAGATGGGAATGGTG+GGG	0.692482	7.1:-81782694	MS.gene021913:CDS
TTGTTGGAAACTGTGTTTGG+GGG	0.709229	7.1:-81783004	MS.gene021913:CDS
TTTGTTGGAAACTGTGTTTG+GGG	0.747905	7.1:-81783005	MS.gene021913:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATAATTCTTTGATAAATGA+AGG	-	chr7.1:81783829-81783848	MS.gene021913:CDS	15.0%
!!	ATAAAGATGATAAATGAGAA+CGG	-	chr7.1:81783769-81783788	MS.gene021913:CDS	20.0%
!!	GTACATCATATATATAATCT+CGG	+	chr7.1:81783326-81783345	None:intergenic	20.0%
!!	TTCTTTCTTCTTTCAAAAAT+TGG	-	chr7.1:81782814-81782833	MS.gene021913:CDS	20.0%
!!!	AATTTTTGAAAGAAGAAAGA+AGG	+	chr7.1:81782815-81782834	None:intergenic	20.0%
!!!	ATTTTTGAAAGAAGAAAGAA+GGG	+	chr7.1:81782814-81782833	None:intergenic	20.0%
!	AAAAAAAGTCGAGATAGTAT+CGG	-	chr7.1:81783924-81783943	MS.gene021913:CDS	25.0%
!	ACAAAAGATGATAAAGTTTG+TGG	+	chr7.1:81783517-81783536	None:intergenic	25.0%
!	TTGGATTTCATAAAAGAAGA+TGG	-	chr7.1:81783712-81783731	MS.gene021913:CDS	25.0%
!!	ATTTAGAACCTTTATTCGAT+AGG	-	chr7.1:81783248-81783267	MS.gene021913:CDS	25.0%
!!	TCTTCTTTTATGAAATCCAA+TGG	+	chr7.1:81783712-81783731	None:intergenic	25.0%
!!	TTTAGAACCTTTATTCGATA+GGG	-	chr7.1:81783249-81783268	MS.gene021913:CDS	25.0%
!!!	AAAAACCACTTTTTGGAAAA+AGG	+	chr7.1:81783102-81783121	None:intergenic	25.0%
!!!	AAAACCACTTTTTGGAAAAA+GGG	+	chr7.1:81783101-81783120	None:intergenic	25.0%
!!!	AATGAAGGATTTAGAGTTTT+TGG	-	chr7.1:81783844-81783863	MS.gene021913:CDS	25.0%
!!!	ATCGAATAAAGGTTCTAAAT+CGG	+	chr7.1:81783248-81783267	None:intergenic	25.0%
	AAACAATCTTACCAGCTAAA+GGG	+	chr7.1:81782886-81782905	None:intergenic	30.0%
	AATATGTCGAAGAAAGTGAA+AGG	+	chr7.1:81782728-81782747	None:intergenic	30.0%
	GAAAGGAATTGTTGTAGTTT+GGG	+	chr7.1:81782711-81782730	None:intergenic	30.0%
	TAGAAGAAGATTCGTTCTAT+AGG	+	chr7.1:81782767-81782786	None:intergenic	30.0%
	TGAAAGGAATTGTTGTAGTT+TGG	+	chr7.1:81782712-81782731	None:intergenic	30.0%
	TTTGAACGAGATAAAGCAAA+TGG	-	chr7.1:81783444-81783463	MS.gene021913:CDS	30.0%
!	ATAACCCTTTTTCCAAAAAG+TGG	-	chr7.1:81783094-81783113	MS.gene021913:CDS	30.0%
!	TCACTTTCTTCGACATATTT+TGG	-	chr7.1:81782729-81782748	MS.gene021913:CDS	30.0%
!!	AGCAAATGGTGTTATCAAAA+TGG	-	chr7.1:81783458-81783477	MS.gene021913:CDS	30.0%
!!	GCAAATGGTGTTATCAAAAT+GGG	-	chr7.1:81783459-81783478	MS.gene021913:CDS	30.0%
!!	TCGAATAAAGGTTCTAAATC+GGG	+	chr7.1:81783247-81783266	None:intergenic	30.0%
!!	TTTATGAGACTGATTTTGGT+TGG	-	chr7.1:81783893-81783912	MS.gene021913:CDS	30.0%
!!	TTTGTTGTGTACTGGATTTT+TGG	+	chr7.1:81782929-81782948	None:intergenic	30.0%
!!!	GATACTATCTCGACTTTTTT+TGG	+	chr7.1:81783925-81783944	None:intergenic	30.0%
!!!	TTATGAGACTGATTTTGGTT+GGG	-	chr7.1:81783894-81783913	MS.gene021913:CDS	30.0%
	AACCATGTTCATCAAAGCTT+GGG	-	chr7.1:81783168-81783187	MS.gene021913:CDS	35.0%
	AATGTAACACCGAGTTCATT+TGG	+	chr7.1:81783289-81783308	None:intergenic	35.0%
	ACTTTGTTGGAAACTGTGTT+TGG	-	chr7.1:81783665-81783684	MS.gene021913:CDS	35.0%
	AGAAGATTCGTTCTATAGGA+TGG	+	chr7.1:81782763-81782782	None:intergenic	35.0%
	ATCAAAGACCCAAATGAACT+CGG	-	chr7.1:81783277-81783296	MS.gene021913:CDS	35.0%
	ATCCAGTACACAACAAATGA+TGG	-	chr7.1:81782932-81782951	MS.gene021913:CDS	35.0%
	ATGACTTCCCTATCGAATAA+AGG	+	chr7.1:81783259-81783278	None:intergenic	35.0%
	ATGATGTACCCAAACGAAAA+AGG	-	chr7.1:81783337-81783356	MS.gene021913:CDS	35.0%
	ATGTAACACCGAGTTCATTT+GGG	+	chr7.1:81783288-81783307	None:intergenic	35.0%
	CAAACAATCTTACCAGCTAA+AGG	+	chr7.1:81782887-81782906	None:intergenic	35.0%
	CAGTTTCCAACAAAGTTACT+TGG	+	chr7.1:81783661-81783680	None:intergenic	35.0%
	CCAACAAAGTTACTTGGTAT+TGG	+	chr7.1:81783655-81783674	None:intergenic	35.0%
	CCAATACCAAGTAACTTTGT+TGG	-	chr7.1:81783652-81783671	MS.gene021913:CDS	35.0%
	CTTTAGCTGGTAAGATTGTT+TGG	-	chr7.1:81782885-81782904	MS.gene021913:CDS	35.0%
	CTTTGTTGGAAACTGTGTTT+GGG	-	chr7.1:81783666-81783685	MS.gene021913:CDS	35.0%
	GAGAGTGAAGATTTGAGAAT+TGG	+	chr7.1:81782842-81782861	None:intergenic	35.0%
	GTGTTACATTCACAAATAGC+TGG	-	chr7.1:81783299-81783318	MS.gene021913:CDS	35.0%
	TCTAAATCGGGTAACAAAGT+TGG	+	chr7.1:81783235-81783254	None:intergenic	35.0%
	TTTGGTTGTTGAGATTCTGA+TGG	+	chr7.1:81782911-81782930	None:intergenic	35.0%
	TTTGTTGGAAACTGTGTTTG+GGG	-	chr7.1:81783667-81783686	MS.gene021913:CDS	35.0%
!	AAGGGTGTGATTGTGAATTT+GGG	+	chr7.1:81782796-81782815	None:intergenic	35.0%
!	ACAAAGTTACTTGGTATTGG+TGG	+	chr7.1:81783652-81783671	None:intergenic	35.0%
!	CCAAAAAGTGGTTTTTCCAT+TGG	-	chr7.1:81783106-81783125	MS.gene021913:CDS	35.0%
!	TAGAGGTTTAACCATTGGTT+TGG	-	chr7.1:81783951-81783970	MS.gene021913:CDS	35.0%
!	TGATGATTTTCCATCAAGAC+CGG	+	chr7.1:81783149-81783168	None:intergenic	35.0%
!!	AGGATTTAGAGTTTTTGGAG+TGG	-	chr7.1:81783849-81783868	MS.gene021913:CDS	35.0%
!!	CAAAGTTGGTGATTCTTCTT+TGG	+	chr7.1:81783221-81783240	None:intergenic	35.0%
!!	CCAATGGAAAAACCACTTTT+TGG	+	chr7.1:81783109-81783128	None:intergenic	35.0%
!!	GAACAACTTTGAAGTGTTCA+TGG	+	chr7.1:81782676-81782695	None:intergenic	35.0%
!!	GAATCTGTTGATTCCAAATG+TGG	+	chr7.1:81783049-81783068	None:intergenic	35.0%
!!	GGTGTTTATGAGACTGATTT+TGG	-	chr7.1:81783889-81783908	MS.gene021913:CDS	35.0%
!!	GTTCGTTTCCTTTTTCGTTT+GGG	+	chr7.1:81783348-81783367	None:intergenic	35.0%
!!!	ATTTAGAGTTTTTGGAGTGG+CGG	-	chr7.1:81783852-81783871	MS.gene021913:CDS	35.0%
!!!	TATGAGACTGATTTTGGTTG+GGG	-	chr7.1:81783895-81783914	MS.gene021913:CDS	35.0%
	AATGAGAACGGTGTTCTTGA+AGG	-	chr7.1:81783781-81783800	MS.gene021913:CDS	40.0%
	ATGAGAACGGTGTTCTTGAA+GGG	-	chr7.1:81783782-81783801	MS.gene021913:CDS	40.0%
	ATGGTGTGTGCTTATTCCAA+TGG	+	chr7.1:81783125-81783144	None:intergenic	40.0%
	CAACCATGTTCATCAAAGCT+TGG	-	chr7.1:81783167-81783186	MS.gene021913:CDS	40.0%
	CTTCATCGATACACAACCAT+TGG	-	chr7.1:81783693-81783712	MS.gene021913:CDS	40.0%
	CTTGAAGGGGCTATTAATGA+CGG	-	chr7.1:81783796-81783815	MS.gene021913:CDS	40.0%
	GTGGATAGAGGTTTAACCAT+TGG	-	chr7.1:81783946-81783965	MS.gene021913:CDS	40.0%
	GTGTGATTGTGAATTTGGGA+GGG	+	chr7.1:81782792-81782811	None:intergenic	40.0%
	TAACCTTGCTCTGCAATCTA+CGG	+	chr7.1:81783626-81783645	None:intergenic	40.0%
	TCGTTCGTTTCTACCACATT+TGG	-	chr7.1:81783033-81783052	MS.gene021913:CDS	40.0%
	TCTCTTTCTCCATATCAGCT+CGG	+	chr7.1:81783585-81783604	None:intergenic	40.0%
	TTGAAGGGGCTATTAATGAC+GGG	-	chr7.1:81783797-81783816	MS.gene021913:CDS	40.0%
	TTGTTGGAAACTGTGTTTGG+GGG	-	chr7.1:81783668-81783687	MS.gene021913:CDS	40.0%
!	AAAAGTCGAGATAGTATCGG+TGG	-	chr7.1:81783927-81783946	MS.gene021913:CDS	40.0%
!	GAAGGGTGTGATTGTGAATT+TGG	+	chr7.1:81782797-81782816	None:intergenic	40.0%
!!	CACCATCATTTGTTGTGTAC+TGG	+	chr7.1:81782937-81782956	None:intergenic	40.0%
!!	CGTTCGTTTCCTTTTTCGTT+TGG	+	chr7.1:81783349-81783368	None:intergenic	40.0%
!!	GTAGTTTGGGTTGATGATGA+AGG	+	chr7.1:81782698-81782717	None:intergenic	40.0%
!!	TAAAGGGAGAAAGTGTTGGA+GGG	+	chr7.1:81782870-81782889	None:intergenic	40.0%
!!!	TTTAGAGTTTTTGGAGTGGC+GGG	-	chr7.1:81783853-81783872	MS.gene021913:CDS	40.0%
	AGAGCAAAGATGGGAATGGT+GGG	-	chr7.1:81783977-81783996	MS.gene021913:CDS	45.0%
	ATCAGACTCTGCAATGAGCA+AGG	+	chr7.1:81782963-81782982	None:intergenic	45.0%
	CAACACTTTCTCCCTTTAGC+TGG	-	chr7.1:81782872-81782891	MS.gene021913:CDS	45.0%
	CGAGACAAAGAAGCTTCATG+AGG	+	chr7.1:81783016-81783035	None:intergenic	45.0%
	GGTGTGATTGTGAATTTGGG+AGG	+	chr7.1:81782793-81782812	None:intergenic	45.0%
	GTTTGGCAGAGAGCAAAGAT+GGG	-	chr7.1:81783968-81783987	MS.gene021913:CDS	45.0%
	TGAGAACGGTGTTCTTGAAG+GGG	-	chr7.1:81783783-81783802	MS.gene021913:CDS	45.0%
	TTTGCTCTCTGCCAAACCAA+TGG	+	chr7.1:81783965-81783984	None:intergenic	45.0%
!	AGCCCAAGCTTTGATGAACA+TGG	+	chr7.1:81783173-81783192	None:intergenic	45.0%
!	ATTTTCCATCAAGACCGGCA+TGG	+	chr7.1:81783144-81783163	None:intergenic	45.0%
!	CAGCTAAAGGGAGAAAGTGT+TGG	+	chr7.1:81782874-81782893	None:intergenic	45.0%
!	GTGGCGGGATCTAATAGATT+TGG	-	chr7.1:81783868-81783887	MS.gene021913:CDS	45.0%
!!	CGAACGGAGTCTTCAAGTTT+TGG	+	chr7.1:81783397-81783416	None:intergenic	45.0%
!!	CTAAAGGGAGAAAGTGTTGG+AGG	+	chr7.1:81782871-81782890	None:intergenic	45.0%
!!	GAGATAGTATCGGTGGATAG+AGG	-	chr7.1:81783934-81783953	MS.gene021913:CDS	45.0%
!!!	AGACTGATTTTGGTTGGGGA+AGG	-	chr7.1:81783899-81783918	MS.gene021913:CDS	45.0%
	AGCCATTCACCGAGCTGATA+TGG	-	chr7.1:81783573-81783592	MS.gene021913:CDS	50.0%
	GAGAGCAAAGATGGGAATGG+TGG	-	chr7.1:81783976-81783995	MS.gene021913:CDS	50.0%
	GAGATCGAATGTGGCACGAA+CGG	+	chr7.1:81783413-81783432	None:intergenic	50.0%
	GAGCAAAGATGGGAATGGTG+GGG	-	chr7.1:81783978-81783997	MS.gene021913:CDS	50.0%
	GCAGAGAGCAAAGATGGGAA+TGG	-	chr7.1:81783973-81783992	MS.gene021913:CDS	50.0%
	GGAATAAGCACACACCATGC+CGG	-	chr7.1:81783127-81783146	MS.gene021913:CDS	50.0%
	GGTTTGGCAGAGAGCAAAGA+TGG	-	chr7.1:81783967-81783986	MS.gene021913:CDS	50.0%
	TCACCGTAGATTGCAGAGCA+AGG	-	chr7.1:81783620-81783639	MS.gene021913:CDS	50.0%
	TTCACGCGTGAGATCGAATG+TGG	+	chr7.1:81783422-81783441	None:intergenic	50.0%
!	CTCCATATCAGCTCGGTGAA+TGG	+	chr7.1:81783578-81783597	None:intergenic	50.0%
	ACACACCATGCCGGTCTTGA+TGG	-	chr7.1:81783136-81783155	MS.gene021913:CDS	55.0%
!	GGGAATGGTGGGGTTGAAGT+TGG	-	chr7.1:81783988-81784007	MS.gene021913:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	81782644	81784050	81782644	ID=MS.gene021913
chr7.1	mRNA	81782644	81784050	81782644	ID=MS.gene021913.t1;Parent=MS.gene021913
chr7.1	exon	81782644	81784050	81782644	ID=MS.gene021913.t1.exon1;Parent=MS.gene021913.t1
chr7.1	CDS	81782644	81784050	81782644	ID=cds.MS.gene021913.t1;Parent=MS.gene021913.t1

Gene Sequence

>MS.gene021913

ATGGCATTTAACAAGAACAATATCAAAATCCATGAACACTTCAAAGTTGTTCCTTCATCATCAACCCAAACTACAACAATTCCTTTCACTTTCTTCGACATATTTTGGATAAAGTTCCATCCTATAGAACGAATCTTCTTCTACACCCTCCCAAATTCACAATCACACCCTTCTTTCTTCTTTCAAAAATTGGTTCCAATTCTCAAATCTTCACTCTCTTTAACCCTCCAACACTTTCTCCCTTTAGCTGGTAAGATTGTTTGGCCATCAGAATCTCAACAACCAAAAATCCAGTACACAACAAATGATGGTGTTTCCTTGCTCATTGCAGAGTCTGATGCAAATTTCAATCATGTCATTGAAAACTCACCTCATGAAGCTTCTTTGTCTCGTTCGTTTCTACCACATTTGGAATCAACAGATTCATTTGCTTCTATTATTTCAATTCAGATAACCCTTTTTCCAAAAAGTGGTTTTTCCATTGGAATAAGCACACACCATGCCGGTCTTGATGGAAAATCATCAACCATGTTCATCAAAGCTTGGGCTTATCTATGCAACAAAACAATTGAAACCAAAGAAGAATCACCAACTTTGTTACCCGATTTAGAACCTTTATTCGATAGGGAAGTCATCAAAGACCCAAATGAACTCGGTGTTACATTCACAAATAGCTGGACCGAGATTATATATATGATGTACCCAAACGAAAAAGGAAACGAACGTAGCTTAAATATTTTACACTTTGAACCAAAACTTGAAGACTCCGTTCGTGCCACATTCGATCTCACGCGTGAAGATTTGAACGAGATAAAGCAAATGGTGTTATCAAAATGGGATATTTTTGATACAAATCAATCATATTCAAAGCCACAAACTTTATCATCTTTTGTTCTCACTTGTGCTTATTCACTCGTTTGTTATGCAAAAGCCATTCACCGAGCTGATATGGAGAAAGAGAAGTTTTCTTTTGTTTTCACCGTAGATTGCAGAGCAAGGTTAGAACCACCAATACCAAGTAACTTTGTTGGAAACTGTGTTTGGGGGAACTTCATCGATACACAACCATTGGATTTCATAAAAGAAGATGGTGTTTTTTTAGTTGCTAAGTGTATTTACGAGAAAATAAAGATGATAAATGAGAACGGTGTTCTTGAAGGGGCTATTAATGACGGGTATGATAAGTATAATTCTTTGATAAATGAAGGATTTAGAGTTTTTGGAGTGGCGGGATCTAATAGATTTGGTGTTTATGAGACTGATTTTGGTTGGGGAAGGCCAAAAAAAGTCGAGATAGTATCGGTGGATAGAGGTTTAACCATTGGTTTGGCAGAGAGCAAAGATGGGAATGGTGGGGTTGAAGTTGGTCTTGTTCTTAATAAGCACGTATATAGCAAGTGTACCTAG

Protein sequence

>MS.gene021913.t1

MAFNKNNIKIHEHFKVVPSSSTQTTTIPFTFFDIFWIKFHPIERIFFYTLPNSQSHPSFFFQKLVPILKSSLSLTLQHFLPLAGKIVWPSESQQPKIQYTTNDGVSLLIAESDANFNHVIENSPHEASLSRSFLPHLESTDSFASIISIQITLFPKSGFSIGISTHHAGLDGKSSTMFIKAWAYLCNKTIETKEESPTLLPDLEPLFDREVIKDPNELGVTFTNSWTEIIYMMYPNEKGNERSLNILHFEPKLEDSVRATFDLTREDLNEIKQMVLSKWDIFDTNQSYSKPQTLSSFVLTCAYSLVCYAKAIHRADMEKEKFSFVFTVDCRARLEPPIPSNFVGNCVWGNFIDTQPLDFIKEDGVFLVAKCIYEKIKMINENGVLEGAINDGYDKYNSLINEGFRVFGVAGSNRFGVYETDFGWGRPKKVEIVSVDRGLTIGLAESKDGNGGVEVGLVLNKHVYSKCT